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Encapsulation of Cinnamon and Thyme Essential Oils in γ-Cyclodextrin: Studies on Tribology and Rheology of Diluted Emulsions and Morphologies of Encapsulates 肉桂和百里香精油在γ-环糊精中的封装:稀释乳液的摩擦学和流变学以及封装物形态的研究
IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-30 DOI: 10.1021/acsfoodscitech.4c0024110.1021/acsfoodscitech.4c00241
Jasim Ahmed*, Abdullah Alazemi and Bini T. B., 

Essential oils (EO) are extensively used in food and pharmaceutical formulations for their exotic flavor and antimicrobial properties. The objectives of this work were to develop a cinnamon essential oil (CEO) and thyme essential oil (TEO) inclusion complex using γ-cyclodextrin (GCD) as a wall material at a mass ratio of GCD to EO 80 to 20. The main focus of the work was to understand the flow and frictional behaviors of the individual EOs and their emulsions/solutions using the tribological and rheological behavior prior to transformation into dry encapsulates. EOs exhibited non-Newtonian shear-thickening behavior with yield stress. The Stribeck curves exhibited the lubricating effects of EOs during friction tests. The addition of GCD to the solution caused larger friction coefficients in mixed and hydrodynamic lubrication regions. The surface morphology of emulsions displayed larger wear in the balls and disks of the tribometer. X-ray diffraction (XRD) and thermogravimetric analysis (TGA) analysis confirmed the formation of stable encapsulates between GCD and EOs, with the appearance of new peaks confirming an improvement in thermal stability. Surface morphology data indicated an increase in height parameters and a difference in roughness between two encapsulates. The obtained results can be helpful in food and drug formulation and their delivery systems.

精油(EO)因其奇特的风味和抗菌特性被广泛应用于食品和药物配方中。这项研究的目的是以γ-环糊精(GCD)为壁材,开发肉桂精油(CEO)和百里香精油(TEO)的包合复合物,GCD 与 EO 的质量比为 80:20。这项工作的主要重点是利用转化为干包合物之前的摩擦学和流变学行为,了解单个环氧乙烷及其乳液/溶液的流动和摩擦行为。环氧乙烷在屈服应力作用下表现出非牛顿剪切增稠行为。斯特里贝克曲线显示了 EO 在摩擦试验中的润滑效果。在溶液中添加 GCD 会导致混合润滑区和流体动力润滑区的摩擦系数增大。乳化液的表面形态显示摩擦磨损计的球和盘磨损较大。X 射线衍射 (XRD) 和热重分析 (TGA) 分析证实,GCD 和 EO 之间形成了稳定的包合物,新峰值的出现证实了热稳定性的提高。表面形态数据表明,两种封装物的高度参数有所增加,粗糙度也有差异。所得结果有助于食品和药物配方及其输送系统。
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引用次数: 0
Effect of the Molecular Structure and Degree of Polymerization of Fructans on the Growth Behavior of Four Lactic Acid Bacteria 果聚糖的分子结构和聚合度对四种乳酸菌生长行为的影响
IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-29 DOI: 10.1021/acsfoodscitech.4c0025110.1021/acsfoodscitech.4c00251
Juan L. Morales-Landa, Lorena Moreno-Vilet, Luis M. Cordero-Quiñones, José A. González-Montemayor, Jorge A. García-Fajardo and Noé Luiz-Santos*, 

The effects of linear and branched fructans from chicory and agave, respectively, and of different degrees of polymerization (DP) were evaluated on the growth curves of Lactobacillus acidophilus, Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, and Lactobacillus rhamnosus. Native fructans from agaves were separated into fractions with different DPs using the ultrafiltration process. The fructan profile was determined by size exclusion chromatography (high-performance liquid chromatography (HPLC)-SEC) and grouped according to their DPs. The growth was evaluated by the specific growth rate (μ) using turbidimetry (nephelometric turbidity unit (NTU)) at 860 nm for 12–22 h in modified De Man–Rogosa–Sharpe (MRS) broth, using fructans as a carbohydrate source. The different tested strains exhibited varying growth rates under the tested treatments, and the highest growth was obtained with agave fructooligosaccharides (FOS) in a range between 4 and 50% of the four LAB evaluated in comparison with linear fructans, so they can be considered for the development of new functional foods and pharmaceutical industries.

分别从菊苣和龙舌兰中提取的不同聚合度(DP)的线性和支链果聚糖对嗜酸乳杆菌、副酸乳杆菌、植物乳杆菌和鼠李糖乳杆菌生长曲线的影响进行了评估。利用超滤工艺将龙舌兰中的原生果聚糖分离成不同DP的馏分。果聚糖概况通过尺寸排阻色谱法(高效液相色谱法(HPLC)-SEC)进行测定,并根据其 DP 值进行分组。在改良的 De Man-Rogosa-Sharpe (MRS)肉汤中,以果聚糖为碳水化合物来源,在 860 纳米波长下用浊度计(浊度单位(NTU))测定 12-22 小时的特定生长率(μ)来评估生长情况。与线性果聚糖相比,龙舌兰果寡糖(FOS)的生长率最高,在 4% 到 50% 的范围内,四种受测 LAB 的生长率都很高,因此可以考虑将其用于开发新的功能性食品和制药工业。
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引用次数: 0
Effect of the Molecular Structure and Degree of Polymerization of Fructans on the Growth Behavior of Four Lactic Acid Bacteria 果聚糖的分子结构和聚合度对四种乳酸菌生长行为的影响
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-29 DOI: 10.1021/acsfoodscitech.4c00251
Juan L. Morales-Landa, Lorena Moreno-Vilet, Luis M. Cordero-Quiñones, José A. González-Montemayor, Jorge A. García-Fajardo, Noé Luiz-Santos
The effects of linear and branched fructans from chicory and agave, respectively, and of different degrees of polymerization (DP) were evaluated on the growth curves of Lactobacillus acidophilus, Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, and Lactobacillus rhamnosus. Native fructans from agaves were separated into fractions with different DPs using the ultrafiltration process. The fructan profile was determined by size exclusion chromatography (high-performance liquid chromatography (HPLC)-SEC) and grouped according to their DPs. The growth was evaluated by the specific growth rate (μ) using turbidimetry (nephelometric turbidity unit (NTU)) at 860 nm for 12–22 h in modified De Man–Rogosa–Sharpe (MRS) broth, using fructans as a carbohydrate source. The different tested strains exhibited varying growth rates under the tested treatments, and the highest growth was obtained with agave fructooligosaccharides (FOS) in a range between 4 and 50% of the four LAB evaluated in comparison with linear fructans, so they can be considered for the development of new functional foods and pharmaceutical industries.
分别从菊苣和龙舌兰中提取的不同聚合度(DP)的线性和支链果聚糖对嗜酸乳杆菌、副酸乳杆菌、植物乳杆菌和鼠李糖乳杆菌生长曲线的影响进行了评估。利用超滤工艺将龙舌兰中的原生果聚糖分离成不同DP的馏分。果聚糖概况通过尺寸排阻色谱法(高效液相色谱法(HPLC)-SEC)进行测定,并根据其 DP 值进行分组。在改良的 De Man-Rogosa-Sharpe (MRS)肉汤中,以果聚糖为碳水化合物来源,在 860 纳米波长下用浊度计(浊度单位(NTU))测定 12-22 小时的特定生长率(μ)来评估生长情况。与线性果聚糖相比,龙舌兰果寡糖(FOS)的生长率最高,在 4% 到 50% 的范围内,四种受测 LAB 的生长率都很高,因此可以考虑将其用于开发新的功能性食品和制药工业。
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引用次数: 0
Adsorption Kinetics and Interfacial Dilatational Rheology of Oil–Water Interfacial Films Loaded with Homogalacturonan and Rhamnogalacturonan-I 负载高半乳糖醛酸和鼠李糖半乳糖醛酸-I 的油水界面薄膜的吸附动力学和界面扩张流变学
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-26 DOI: 10.1021/acsfoodscitech.4c00357
Hui Niu, Wenduo Wang, Haiming Chen, Xiong Fu
Our recent study on the isolation of rhamnogalacturonan-I (RG-I) pectin enriched with acetyl groups, ferulic acid, neutral sugar side chains, and proteins through enzymatic hydrolysis of the homogalacturonan (HG) region of sugar beet pectin showed that RG-I molecules can form more stable emulsions than HG molecules. In this experiment, the interfacial adsorption kinetics of HG and RG-I molecules at the oil–water interface and the resistance effects of these interfacial films on extrinsic perturbations were further investigated. All of these experimental results indicated that the RG-I-stabilized interfacial film presented better elasticity (43.36 mN/m) and stronger resistance to extrinsic perturbations than HG (12.41 mN/m). In addition, both HG- and RG-I-loaded interfacial films exhibited linear viscoelastic responses when a small interfacial deformation and low-frequency perturbation were applied. When the interfacial deformation increased further, both HG- and RG-loaded interfacial films underwent strain softening during the extension stage. However, in the compression stage, the HG-loaded interfacial film underwent strain hardening, whereas the RG-loaded interfacial film exhibited strain softening.
我们最近通过酶水解甜菜果胶的高半乳糖醛酸(HG)区域,分离出富含乙酰基、阿魏酸、中性糖侧链和蛋白质的鼠李糖半乳糖醛酸-I(RG-I)果胶的研究表明,RG-I 分子比 HG 分子能形成更稳定的乳液。在该实验中,进一步研究了 HG 和 RG-I 分子在油水界面上的界面吸附动力学以及这些界面薄膜对外部扰动的阻力效应。所有这些实验结果都表明,与 HG(12.41 mN/m)相比,RG-I 稳定界面膜具有更好的弹性(43.36 mN/m)和更强的抗外扰动能力。此外,当施加较小的界面变形和低频扰动时,HG 和 RG-I 负载界面薄膜都表现出线性粘弹性响应。当界面变形进一步增大时,HG 和 RG 负载界面薄膜在延伸阶段都发生了应变软化。然而,在压缩阶段,加载了 HG 的界面薄膜发生了应变硬化,而加载了 RG 的界面薄膜则表现出应变软化。
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引用次数: 0
Adsorption Kinetics and Interfacial Dilatational Rheology of Oil–Water Interfacial Films Loaded with Homogalacturonan and Rhamnogalacturonan-I 负载高半乳糖醛酸和鼠李糖半乳糖醛酸-I 的油水界面薄膜的吸附动力学和界面扩张流变学
IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-26 DOI: 10.1021/acsfoodscitech.4c0035710.1021/acsfoodscitech.4c00357
Hui Niu, Wenduo Wang, Haiming Chen* and Xiong Fu*, 

Our recent study on the isolation of rhamnogalacturonan-I (RG-I) pectin enriched with acetyl groups, ferulic acid, neutral sugar side chains, and proteins through enzymatic hydrolysis of the homogalacturonan (HG) region of sugar beet pectin showed that RG-I molecules can form more stable emulsions than HG molecules. In this experiment, the interfacial adsorption kinetics of HG and RG-I molecules at the oil–water interface and the resistance effects of these interfacial films on extrinsic perturbations were further investigated. All of these experimental results indicated that the RG-I-stabilized interfacial film presented better elasticity (43.36 mN/m) and stronger resistance to extrinsic perturbations than HG (12.41 mN/m). In addition, both HG- and RG-I-loaded interfacial films exhibited linear viscoelastic responses when a small interfacial deformation and low-frequency perturbation were applied. When the interfacial deformation increased further, both HG- and RG-loaded interfacial films underwent strain softening during the extension stage. However, in the compression stage, the HG-loaded interfacial film underwent strain hardening, whereas the RG-loaded interfacial film exhibited strain softening.

我们最近通过酶水解甜菜果胶的高半乳糖醛酸(HG)区域,分离出富含乙酰基、阿魏酸、中性糖侧链和蛋白质的鼠李糖半乳糖醛酸-I(RG-I)果胶的研究表明,RG-I 分子比 HG 分子能形成更稳定的乳液。在该实验中,进一步研究了 HG 和 RG-I 分子在油水界面上的界面吸附动力学以及这些界面薄膜对外部扰动的阻力效应。所有这些实验结果都表明,与 HG(12.41 mN/m)相比,RG-I 稳定界面膜具有更好的弹性(43.36 mN/m)和更强的抗外扰动能力。此外,当施加较小的界面变形和低频扰动时,HG 和 RG-I 负载界面薄膜都表现出线性粘弹性响应。当界面变形进一步增大时,HG 和 RG 负载界面薄膜在延伸阶段都发生了应变软化。然而,在压缩阶段,加载了 HG 的界面薄膜发生了应变硬化,而加载了 RG 的界面薄膜则表现出应变软化。
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引用次数: 0
A New Alkylamide with Odd-Chain Fatty Acid from Pericarps of Japanese Pepper (Zanthoxylum piperitum (L.) DC.) 一种来自日本胡椒(Zanthoxylum piperitum (L.) DC.)果皮的新型奇链脂肪酸烷基酰胺
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-25 DOI: 10.1021/acsfoodscitech.3c00672
Tomone Kamijima, Rie Kamihira, Fumiaki Nakamura, Yoichi Nakao
Neural stem cells (NSCs) are pluripotent stem cells that can differentiate into neurons and glia cells. They continue to supply these cells throughout life in the adult central nervous system. However, it is revealed that differentiation capacity of NSCs has been to decrease with aging and mental stress. The decreasing capacity suspected to be associated with the development of neurodegenerative diseases and depression. Therefore, compounds that regulate the differentiation of NSCs into astrocytes may provide a good clue to understanding the role of astrocytes in maintaining brain function. In this study, the extracts of the pericarps of Japanese pepper (Zanthoxylum piperitum (L.) DC.), which has long been popular as a spice in Japanese cuisine, were subjected to solvent fractionation, purification and spectral analysis. As a result, an alkylamide with an unusual odd-chain fatty acid moiety, (2E)-N-isobutyltridec-2-enamide (1), was isolated along with three known alkylamides (24). All these alkylamides were evaluated for their promoting activity in the differentiation of mouse neural stem cells (mNSCs) into astrocyte. As the result, compounds 2 and 4 increased numbers of glial fibrillary acidic protein (GFAP)-positive cells in in vitro differentiation assays using mNSCs. These findings suggest the potential for new uses of Japanese pepper as a functional food that could be applied to the prevention and treatment of neurodegenerative diseases and depression.
神经干细胞(NSC)是多能干细胞,可分化为神经元和胶质细胞。它们在成人中枢神经系统的整个生命过程中持续提供这些细胞。然而,有研究表明,随着年龄的增长和精神压力的增加,神经干细胞的分化能力会下降。这种能力的下降被怀疑与神经退行性疾病和抑郁症的发生有关。因此,能调节 NSCs 向星形胶质细胞分化的化合物可能为了解星形胶质细胞在维持大脑功能方面的作用提供了很好的线索。在这项研究中,研究人员对长期以来作为日本料理香料而深受欢迎的日本胡椒(Zanthoxylum piperitum (L.) DC.)的果皮提取物进行了溶剂分馏、纯化和光谱分析。结果,分离出了一种具有不寻常奇链脂肪酸分子的烷基酰胺,即 (2E)-N-isobutyltridec-2-enamide (1),以及三种已知的烷基酰胺 (2-4)。对所有这些烷基酰胺在促进小鼠神经干细胞(mNSCs)向星形胶质细胞分化方面的活性进行了评估。结果发现,在使用 mNSCs 进行的体外分化试验中,化合物 2 和 4 增加了胶质纤维酸性蛋白(GFAP)阳性细胞的数量。这些研究结果表明,日本胡椒有可能作为一种新的功能性食品,用于预防和治疗神经退行性疾病和抑郁症。
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引用次数: 0
Potato Peel as a Natural Source of Biocompounds for Cereal Fungal Control 马铃薯皮是控制谷物真菌的生物化合物天然来源
IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-25 DOI: 10.1021/acsfoodscitech.4c0018910.1021/acsfoodscitech.4c00189
Daniela Pacifico*, Roberto Lo Scalzo, Antonella Calzone, Federica Nicoletti, Bruno Parisi, Helga Cassol, Giulia Bianchi and Chiara Lanzanova, 

UN 2030 Agenda for Sustainable Development and the UN Convention on Biodiversity Strategic Plan for 2020 address the need to reduce synthetic inputs in agroecosystems while decreasing industrial food waste. Peels recovered from processing varieties were profiled, obtaining extracts up to 1200 and 300 μM of total phenols and steroidal glycoalkaloids, respectively. Their antifungal activity was tested by in vitro experiments against Fusarium graminearum and Fusarium verticillioides. A comparison among raw peel extracts, their separated fractions of steroidal glycoalkaloids, phenolic acids, and anthocyanins, their combination, and mixtures of authentic molecules was performed. For all the peels tested, fungal growth was reduced independently of genotype and farming systems with a range from 12 to 30% of radial growth inhibition. While the antifungal role of glycoalkaloids was confirmed (up to 20% inhibition), for the first time, the effectiveness of phenolic acids alone was revealed (up to 30% inhibition).

联合国 2030 年可持续发展议程》和《联合国生物多样性公约 2020 年战略计划》提出,需要减少农业生态系统中的合成投入,同时减少工业食品浪费。对从加工品种中回收的果皮进行了分析,提取物中的总酚和甾体甘氨酰生物碱含量分别高达 1200 和 300 μM。通过体外实验测试了它们对禾谷镰刀菌和轮枝镰刀菌的抗真菌活性。对果皮原提取物、其固醇类糖苷生物碱、酚酸和花青素的分离馏分、它们的组合以及真实分子的混合物进行了比较。在所有测试的果皮中,真菌的生长都有所降低,不受基因型和耕作制度的影响,径向生长抑制率从 12% 到 30% 不等。虽然糖生物碱的抗真菌作用得到了证实(抑制率高达 20%),但酚酸本身的功效(抑制率高达 30%)也首次得到了揭示。
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引用次数: 0
A New Alkylamide with Odd-Chain Fatty Acid from Pericarps of Japanese Pepper (Zanthoxylum piperitum (L.) DC.) 一种来自日本胡椒(Zanthoxylum piperitum (L.) DC.)果皮的新型奇链脂肪酸烷基酰胺
IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-25 DOI: 10.1021/acsfoodscitech.3c0067210.1021/acsfoodscitech.3c00672
Tomone Kamijima, Rie Kamihira, Fumiaki Nakamura and Yoichi Nakao*, 

Neural stem cells (NSCs) are pluripotent stem cells that can differentiate into neurons and glia cells. They continue to supply these cells throughout life in the adult central nervous system. However, it is revealed that differentiation capacity of NSCs has been to decrease with aging and mental stress. The decreasing capacity suspected to be associated with the development of neurodegenerative diseases and depression. Therefore, compounds that regulate the differentiation of NSCs into astrocytes may provide a good clue to understanding the role of astrocytes in maintaining brain function. In this study, the extracts of the pericarps of Japanese pepper (Zanthoxylum piperitum (L.) DC.), which has long been popular as a spice in Japanese cuisine, were subjected to solvent fractionation, purification and spectral analysis. As a result, an alkylamide with an unusual odd-chain fatty acid moiety, (2E)-N-isobutyltridec-2-enamide (1), was isolated along with three known alkylamides (24). All these alkylamides were evaluated for their promoting activity in the differentiation of mouse neural stem cells (mNSCs) into astrocyte. As the result, compounds 2 and 4 increased numbers of glial fibrillary acidic protein (GFAP)-positive cells in in vitro differentiation assays using mNSCs. These findings suggest the potential for new uses of Japanese pepper as a functional food that could be applied to the prevention and treatment of neurodegenerative diseases and depression.

神经干细胞(NSC)是多能干细胞,可分化为神经元和胶质细胞。它们在成人中枢神经系统的整个生命过程中持续提供这些细胞。然而,有研究表明,随着年龄的增长和精神压力的增加,神经干细胞的分化能力会下降。这种能力的下降被怀疑与神经退行性疾病和抑郁症的发生有关。因此,能调节 NSCs 向星形胶质细胞分化的化合物可能为了解星形胶质细胞在维持大脑功能方面的作用提供了很好的线索。在这项研究中,研究人员对长期以来作为日本料理香料而深受欢迎的日本胡椒(Zanthoxylum piperitum (L.) DC.)的果皮提取物进行了溶剂分馏、纯化和光谱分析。结果,分离出了一种具有不寻常奇链脂肪酸分子的烷基酰胺,即 (2E)-N-isobutyltridec-2-enamide (1),以及三种已知的烷基酰胺 (2-4)。对所有这些烷基酰胺在促进小鼠神经干细胞(mNSCs)向星形胶质细胞分化方面的活性进行了评估。结果发现,在使用 mNSCs 进行的体外分化试验中,化合物 2 和 4 增加了胶质纤维酸性蛋白(GFAP)阳性细胞的数量。这些研究结果表明,日本胡椒有可能作为一种新的功能性食品,用于预防和治疗神经退行性疾病和抑郁症。
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引用次数: 0
Development of Spray-Dried Microparticles Loaded by Sour Cherry Pomace Extract: Process Optimization, Characterization, and In Vitro Release Studies 酸樱桃渣提取物载体喷雾干燥微颗粒的开发:工艺优化、表征和体外释放研究
Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-24 DOI: 10.1021/acsfoodscitech.4c00243
İrem Toprakçı, Gülderen Cosgun, Ferhan Balci-Torun, Selin Şahin
The nonfood waste of the sour cherry (Prunus cerasus L.) was used as an active material source in this study. After the active substance of the pomace was extracted by aqueous ethanol, the liquid extract was encapsulated in Arabic gum/maltodextrin (blend or alone) by spray-drying. The microencapsulation formulation was optimized by I-optimal design under combined design with two mixture components (Arabic gum and maltodextrin) and a numeric process factor (inlet temperature). The maximum yields (80.99%, 76.39%, 5.382 mg-TEAC/g-DM, and 0.722 mg-C3G/g-DM) of encapsulation efficiency (EE) in terms of total phenolic content, powder yield (PY), antioxidant activity, and total anthocyanin content (TAC) were achieved by ∼161 °C and Arabic gum alone (10%). Based on the analysis of the variance test, the interaction terms between the Arabic gum ratio and the inlet temperature were statistically significant (p < 0.05) for EE, while every single term was significant at p < 0.0001 in the case of PY. Regarding the antioxidant activity response, each term was significant at p < 0.05. Each term of the TAC analysis was generally statistically important at p < 0.0001. The microparticles were subjected to several morphological tests (particle size distribution, scanning electron microscopy, and diffuse reflectance infrared Fourier transform) and physicochemical measurements (moisture content, water activity, solubility, bulk density, tapped density, and Carr index). Finally, an in vitro digestion test was applied to obtain preliminary information on the developed microparticles before proceeding to the in vivo process.
本研究采用酸樱桃(Prunus cerasus L.)的非食用废弃物作为活性物质来源。用乙醇水溶液提取果渣中的活性物质后,通过喷雾干燥将液体提取物封装在阿拉伯树胶/麦芽糊精(混合或单独)中。微胶囊配方通过 I-optimal 设计进行了优化,采用两种混合成分(阿拉伯树胶和麦芽糊精)和一个数值工艺因子(入口温度)的组合设计。在总酚含量、粉末产量(PY)、抗氧化活性和总花青素含量(TAC)方面,∼161 °C和单独使用阿拉伯树胶(10%)的包囊效率(EE)最高(80.99%、76.39%、5.382 mg-TEAC/g-DM和0.722 mg-C3G/g-DM)。根据方差分析检验,阿拉伯树胶比例和入口温度之间的交互项对 EE 有统计学意义(p < 0.05),而对 PY 来说,每个单项都有显著意义(p < 0.0001)。在抗氧化活性响应方面,每个项在 p < 0.05 时都有显著性。TAC 分析的每个项在 p < 0.0001 时都具有统计学意义。对微粒进行了几种形态测试(粒度分布、扫描电子显微镜和漫反射红外傅立叶变换)和理化测量(含水量、水活性、溶解度、体积密度、敲击密度和卡尔指数)。最后,还进行了体外消化试验,以便在进行体内加工之前获得有关所开发微粒的初步信息。
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引用次数: 0
Development of Spray-Dried Microparticles Loaded by Sour Cherry Pomace Extract: Process Optimization, Characterization, and In Vitro Release Studies 酸樱桃渣提取物载体喷雾干燥微颗粒的开发:工艺优化、表征和体外释放研究
IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-07-24 DOI: 10.1021/acsfoodscitech.4c0024310.1021/acsfoodscitech.4c00243
İrem Toprakçı, Gülderen Cosgun, Ferhan Balci-Torun and Selin Şahin*, 

The nonfood waste of the sour cherry (Prunus cerasus L.) was used as an active material source in this study. After the active substance of the pomace was extracted by aqueous ethanol, the liquid extract was encapsulated in Arabic gum/maltodextrin (blend or alone) by spray-drying. The microencapsulation formulation was optimized by I-optimal design under combined design with two mixture components (Arabic gum and maltodextrin) and a numeric process factor (inlet temperature). The maximum yields (80.99%, 76.39%, 5.382 mg-TEAC/g-DM, and 0.722 mg-C3G/g-DM) of encapsulation efficiency (EE) in terms of total phenolic content, powder yield (PY), antioxidant activity, and total anthocyanin content (TAC) were achieved by ∼161 °C and Arabic gum alone (10%). Based on the analysis of the variance test, the interaction terms between the Arabic gum ratio and the inlet temperature were statistically significant (p < 0.05) for EE, while every single term was significant at p < 0.0001 in the case of PY. Regarding the antioxidant activity response, each term was significant at p < 0.05. Each term of the TAC analysis was generally statistically important at p < 0.0001. The microparticles were subjected to several morphological tests (particle size distribution, scanning electron microscopy, and diffuse reflectance infrared Fourier transform) and physicochemical measurements (moisture content, water activity, solubility, bulk density, tapped density, and Carr index). Finally, an in vitro digestion test was applied to obtain preliminary information on the developed microparticles before proceeding to the in vivo process.

本研究采用酸樱桃(Prunus cerasus L.)的非食用废弃物作为活性物质来源。用乙醇水溶液提取果渣中的活性物质后,通过喷雾干燥将液体提取物封装在阿拉伯树胶/麦芽糊精(混合或单独)中。微胶囊配方通过 I-optimal 设计进行了优化,采用两种混合成分(阿拉伯树胶和麦芽糊精)和一个数值工艺因子(入口温度)的组合设计。在总酚含量、粉末产量(PY)、抗氧化活性和总花青素含量(TAC)方面,∼161 °C和单独使用阿拉伯树胶(10%)的包囊效率(EE)最高(80.99%、76.39%、5.382 mg-TEAC/g-DM和0.722 mg-C3G/g-DM)。根据方差分析检验,阿拉伯树胶比例和入口温度之间的交互项对 EE 有统计学意义(p < 0.05),而对 PY 来说,每个单项都有显著意义(p < 0.0001)。在抗氧化活性响应方面,每个项在 p < 0.05 时都有显著性。TAC 分析的每个项在 p < 0.0001 时都具有统计学意义。对微粒进行了几种形态测试(粒度分布、扫描电子显微镜和漫反射红外傅立叶变换)和理化测量(含水量、水活性、溶解度、体积密度、敲击密度和卡尔指数)。最后,还进行了体外消化试验,以便在进行体内加工之前获得有关所开发微粒的初步信息。
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