Pub Date : 2024-03-01DOI: 10.1016/j.bpr.2024.100155
Adrián Gómez-Sánchez, O. Devos, Raffaele Vitale, Michel Sliwa, Damir Sakhapo, Jörg Enderlein, Anna de Juan, C. Ruckebusch
{"title":"Blind Instrument Response Function Identification from Fluorescence Decays","authors":"Adrián Gómez-Sánchez, O. Devos, Raffaele Vitale, Michel Sliwa, Damir Sakhapo, Jörg Enderlein, Anna de Juan, C. Ruckebusch","doi":"10.1016/j.bpr.2024.100155","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100155","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140274483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.1016/j.bpr.2024.100150
Saskia Altmaier, I. Meiser, F. Stracke, Heiko Zimmermann
{"title":"Diffusion kinetics & perfusion times in tissue models obtained by bioorthogonal Raman μ -spectroscopy","authors":"Saskia Altmaier, I. Meiser, F. Stracke, Heiko Zimmermann","doi":"10.1016/j.bpr.2024.100150","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100150","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140268050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.1016/j.bpr.2024.100145
Sara Anselmo, Giuseppe Sancataldo, V. Vetri
{"title":"Deciphering Amyloid Fibril Molecular Maturation through FLIM-Phasor Analysis of Thioflavin T","authors":"Sara Anselmo, Giuseppe Sancataldo, V. Vetri","doi":"10.1016/j.bpr.2024.100145","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100145","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139816592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.1016/j.bpr.2024.100146
Mohamad Toutounji
{"title":"Empirical Asymmetric Phonon Sideband Due to Phonons in The Protein Matrix Present in Photosynthetic Complexes: Time-Domain Response Theory","authors":"Mohamad Toutounji","doi":"10.1016/j.bpr.2024.100146","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100146","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139830045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.1016/j.bpr.2024.100146
Mohamad Toutounji
{"title":"Empirical Asymmetric Phonon Sideband Due to Phonons in The Protein Matrix Present in Photosynthetic Complexes: Time-Domain Response Theory","authors":"Mohamad Toutounji","doi":"10.1016/j.bpr.2024.100146","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100146","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139889928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.1016/j.bpr.2024.100145
Sara Anselmo, Giuseppe Sancataldo, V. Vetri
{"title":"Deciphering Amyloid Fibril Molecular Maturation through FLIM-Phasor Analysis of Thioflavin T","authors":"Sara Anselmo, Giuseppe Sancataldo, V. Vetri","doi":"10.1016/j.bpr.2024.100145","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100145","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139876645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1016/j.bpr.2024.100144
Neville S. Gilhooly, S. Kowalczykowski
{"title":"Specific anchoring of large topologically closed DNA for single-molecule protein:DNA interactions","authors":"Neville S. Gilhooly, S. Kowalczykowski","doi":"10.1016/j.bpr.2024.100144","DOIUrl":"https://doi.org/10.1016/j.bpr.2024.100144","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139639383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-03eCollection Date: 2023-12-13DOI: 10.1016/j.bpr.2023.100136
{"title":"Thank you to our reviewers.","authors":"","doi":"10.1016/j.bpr.2023.100136","DOIUrl":"https://doi.org/10.1016/j.bpr.2023.100136","url":null,"abstract":"","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10746389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139032867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-01DOI: 10.1016/j.bpr.2023.100135
Mario Bernardi, Francesco Cardarelli
This paper introduces an innovative approach utilizing Google Colaboratory (Colab) for the versatile analysis of phasor Fluorescence Lifetime Imaging Microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in various input file formats. In fact, phasor-FLIM widespread adoption has been hampered by complex instrumentation and data analysis requirements. We mean to make advanced FLIM analysis more accessible to researchers through a cloud-based solution that i) harnesses robust computational resources, ii) eliminates hardware limitations, iii) supports both CPU and GPU processing, We envision a paradigm shift in FLIM data accessibility and potential, aligning with the evolving field of AI-driven FLIM analysis. This approach simplifies FLIM data handling and opens doors for diverse applications, from studying cellular metabolism to investigating drug encapsulation, benefiting researchers across multiple domains. The comparative analysis of freely distributed FLIM tools highlights the unique advantages of this approach in terms of adaptability, scalability, and open-source nature.
{"title":"Phasor Identifier: A Cloud-based Analysis of Phasor-FLIM Data on Python Notebooks","authors":"Mario Bernardi, Francesco Cardarelli","doi":"10.1016/j.bpr.2023.100135","DOIUrl":"https://doi.org/10.1016/j.bpr.2023.100135","url":null,"abstract":"This paper introduces an innovative approach utilizing Google Colaboratory (Colab) for the versatile analysis of phasor Fluorescence Lifetime Imaging Microscopy (FLIM) data collected from various samples (e.g., cuvette, cells, tissues) and in various input file formats. In fact, phasor-FLIM widespread adoption has been hampered by complex instrumentation and data analysis requirements. We mean to make advanced FLIM analysis more accessible to researchers through a cloud-based solution that i) harnesses robust computational resources, ii) eliminates hardware limitations, iii) supports both CPU and GPU processing, We envision a paradigm shift in FLIM data accessibility and potential, aligning with the evolving field of AI-driven FLIM analysis. This approach simplifies FLIM data handling and opens doors for diverse applications, from studying cellular metabolism to investigating drug encapsulation, benefiting researchers across multiple domains. The comparative analysis of freely distributed FLIM tools highlights the unique advantages of this approach in terms of adaptability, scalability, and open-source nature.","PeriodicalId":72402,"journal":{"name":"Biophysical reports","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135509635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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