Clinical complementary medicine and pharmacology最新文献

{"title":"May Traditional Chinese Medicine Reduce the Risk of Developing Rheumatoid Arthritis? An One-armed Prospective Study","authors":"Kaixin Gao ,&nbsp;Qi Liang ,&nbsp;Dongyun Li ,&nbsp;Chuanning Li ,&nbsp;Maojie Wang ,&nbsp;Xiumin Chen ,&nbsp;Qingchun Huang ,&nbsp;Zehuai Wen ,&nbsp;Per-Johan Jakobsson ,&nbsp;Runyue Huang","doi":"10.1016/j.ccmp.2023.100127","DOIUrl":"10.1016/j.ccmp.2023.100127","url":null,"abstract":"<div><h3>Background</h3><p>Rheumatoid arthritis (RA) is a worldwide public health problem. Intervention and prevention before the onset of rheumatic diseases is a new direction in current research.</p></div><div><h3>Objective</h3><p>The aim of this study was to evaluate the potential and feasibility of traditional Chinese medicine (TCM) in the prevention of RA.</p></div><div><h3>Methods</h3><p>This was a single-armed prospective clinical trial. All participants were recruited from a single center in Guangdong, China. Adults who were tested positive for anti-cyclic citrullinated peptide antibody (anti-CCP) and/or rheumatoid factor (RF), had no synovitis and had never been treated with disease-modifying anti-rheumatic drugs (DMARDs), were enrolled to take the Huayu-Qiangshen-Tongbi (HQT) decoction orally twice daily, 200 mL each time for 24 weeks. Primary outcome was the proportion of patients who met 2010 ACR(American College of Rheumatology)/EULAR(European League Against Rheumatism) classification criteria of RA during observation. Secondary outcomes included levels of anti-CCP, RF, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), assessment of signs and symptoms, and radiographic progression by magnetic resonance imaging (MRI).</p></div><div><h3>Results</h3><p>19 individuals were enrolled in the study, 4 of which withdrew because of the epidemic of COVID-19. During the observation period, 3 individuals (20%) developed RA and they had longer morning stiffness (<em>P</em> = 0.009) and more obvious synovial enhancement in MRI (<em>P</em> = 0.041) at baseline when compared with those who did not develop RA. After 24 weeks of intervention, there were improvements in 28-swollen joint count (SJC28) (<em>P</em> = 0.046), Visual Analog Scale (VAS) (<em>P</em> = 0.019), Patient's Global Assessment (PtGA) (<em>P</em> = 0.019) and Physician's Global Assessment (PGA) (<em>P</em> = 0.031), but no statistical significance was observed in the levels of anti-CCP, RF, ESR, CRP, morning stiffness, 28-tender joint count (TJC28), Health Assessment Questionnaire (HAQ) and magnetic resonance imaging (MRI) analysis (<em>P</em> &gt; 0.05).</p></div><div><h3>Conclusion</h3><p>The HQT formula is safe and could improve joint symptoms and signs in these at-risk individuals, but it remains to be investigated in futher study to see if it might potentially reduce the risk of developing RA. Besides, for individuals at high risk to develop RA, morning stiffness and synovial enhancement in MRI might be predictive factors and warning signs.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100127"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371223000499/pdfft?md5=9c15098abffb935ec2f2935764dc83dc&pid=1-s2.0-S2772371223000499-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139538516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture Alleviates HIF1-α-mediated Early Mitophagy in Spinal Cord Injury","authors":"Rong Hu ,&nbsp;Xingying Wu ,&nbsp;Kelin He ,&nbsp;Mengting Shi ,&nbsp;Haipeng Xu ,&nbsp;Yi Chen ,&nbsp;Bowen Chen ,&nbsp;Lei Wu ,&nbsp;Ruijie Ma ,&nbsp;Kang Liang","doi":"10.1016/j.ccmp.2023.100103","DOIUrl":"10.1016/j.ccmp.2023.100103","url":null,"abstract":"<div><h3>Background</h3><p>The inhibitory microenvironment around spinal cord injury (SCI) severely restricted functional repair after injury. Mitophagy was one of the important measures to maintain cellular homeostasis and ensure the harmonious nerve cell microenvironment. Hypoxia-inducible factor1α (HIF1-α) can mediate mitochondrial autophagy in neurodegenerative diseases, but the mechanisms are complex and diverse, which need to be further elucidated. Electroacupuncture plays a significant role in improving the neural microenvironment after spinal cord injury, promote long-term neurological function recovery in SCI patients, but whether electroacupuncture can participate in HIF1-α mediated mitophagy remains unknown.</p></div><div><h3>Objective</h3><p>Investigated the effects of HIF1-α on mitochondrial autophagy in rats with spinal cord contusion and the potential mechanism of electroacupuncture.</p></div><div><h3>Methods</h3><p>Following the successful construction of an SCI model of Sprague-Dawley rat utilizing a modified Allen method, electroacupuncture intervention was performed at T9 and T11 Jiaji acupoint (EX-B2), with further molecular biology and morphology examined by perfusion. To observe the effect of HIF1-α on local damage repair, the stereotypic injection of <em>Hif1a</em> knockdown virus was performed, and the changes of mitophagy in damaged local area was detected employing Western blotting, real-time fluorescence quantitative PCR, immunofluorescence, transmission electron microscopy and Nissl staining.</p></div><div><h3>Results</h3><p>HIF1-α as well as its mitophagy receptor BNIP3 and NIX are upregulated after spinal cord injury. Electroacupuncture treatment or local inhibition of HIF1-α expression can reverse the early autophagy state after spinal cord injury, reduce cell apoptosis and injury area, promote neuronal survival.</p></div><div><h3>Conclusion</h3><p>Electroacupuncture may serve as a promising strategy for spinal cord injury treatment, by alleviating HIF1-α mediated early mitochondrial autophagy.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44860318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-aging Related Activities and Health Benefits of Licochalcone A: A Review","authors":"Iffat Ara ,&nbsp;Rita Turcio ,&nbsp;Tasmia Islam ,&nbsp;Md. Sabbir Hossain ,&nbsp;Md. Kamrul Hasan","doi":"10.1016/j.ccmp.2023.100125","DOIUrl":"10.1016/j.ccmp.2023.100125","url":null,"abstract":"<div><h3>Background</h3><p>Licochalcone A (LCA) is a flavonoid derived from the roots of <em>Glycyrrhiza uralensis</em> Fisch. ex DC and other related plants. Its natural source has been extensively used in complementary medicine, especially traditional Chinese medicine, to treat various ailments. Identified as a phenolic chalcone compound, LCA has gained significant attention in recent years due to its various pharmacological properties.</p></div><div><h3>Objective</h3><p>The objective of this review article is to assess the auti-aging ralated pharmacological properties of licochalcone A.</p></div><div><h3>Method</h3><p>An exhaustive search of several scientific databases was conducted using various relevant keywords to write this review article on LCA, focusing on its therapeutic applications and anti-aging-related pharmacological activities. Some inclusion and exclusion criteria were strictly followed to retrieve updated relevant articles.</p></div><div><h3>Results</h3><p>A collection of 292 papers were screened, and 131 were included in this review for an in-depth analysis of Licochalcone A, focusing on its anti-aging-related pharmacological activities and relevant mechanisms, and its toxicity and side effects. Moreover, the potential of LCA as a pharmacological product is discussed, emphasizing its health benefits and potential as a pharmaceutical product.</p></div><div><h3>Conclusion</h3><p>These findings indicate that Licochalcone A is a promising natural therapeutic agent for anti-aging therapy and other ailments.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100125"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371223000475/pdfft?md5=ffadd1e383b2b65150f961c2209c83dc&pid=1-s2.0-S2772371223000475-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138621916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GSZ Formula Enhances Sleep Quality: Exploring Its Active Ingredients and Mechanism Using a Network Medicine Approach","authors":"Airong Ren ,&nbsp;Mingxuan Ma ,&nbsp;Yongyin Liang ,&nbsp;Yarong Wang ,&nbsp;Zhengkun Li ,&nbsp;Yahui Liu ,&nbsp;Qing Fan ,&nbsp;Guozhen Cui","doi":"10.1016/j.ccmp.2023.100107","DOIUrl":"10.1016/j.ccmp.2023.100107","url":null,"abstract":"<div><h3>Background</h3><p>Sleep is essential for maintaining human health, and insomnia is a widespread problem. Traditional Chinese medicine (TCM) has been used for centuries to treat sleep disorders, with fewer reported side effects compared to conventional treatments.</p></div><div><h3>Objective</h3><p>This study seeks to investigate the sleep-promoting effects of the GSZ formula, which comprises <em>γ</em>-aminobutyric acid (GABA), Schisandrae Chinensis Fructus (Wuweizi in Chinese), and Ziziphi Spinosae Semen (Suanzaoren in Chinese). In addition, we aim to explore the active ingredients and potential mechanisms underlying the sleep-enhancing effects of the formula.</p></div><div><h3>Methods</h3><p>The impact of GSZ on sleep was evaluated using two models, the complete sleep model and the subthreshold sleep model. Mice were randomly divided into five groups and orally administered GSZ solution (0.33 g/kg/day or 0.99 g/kg/day), positive drug diazepam (2.50 mg/kg) or a control solution for 30 days. Hypnosis model was established in mice using pentobarbital sodium. Sleep duration and incidence were measured by recording when the righting reflex of mice disappeared for more than 1 min. GABA and dopamine (DA) levels in mouse brain tissue were measured using ELISA kits. The ingredients of the GSZ formula were identified using mass spectrometry, and the targets of these ingredients and disease-related genes were retrieved from public databases. A network medicine approach was used to calculate the shortest path between ingredient targets and disease-related proteins. The expression levels of potential proteins, such as Akt, p-Akt, GSK-3β, and p-GSK-3β, were analyzed using Western blotting based on the predicted results.</p></div><div><h3>Results</h3><p>GSZ significantly prolonged sleep duration and enhanced the sleep rate in mice (<em>P</em> &lt; 0.05). Furthermore, it elevated GABA levels and reduced DA levels in the mouse brain (<em>P</em> &lt; 0.05). Network medicine analysis suggested that GABA, stearic acid, genistin, and coumestrol may be the most crucial active ingredients for sleep improvement. Western blotting analysis demonstrated that GSZ modulated the protein expression levels of p-Akt/Akt and p-GSK-3β/GSK-3β (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>Our study demonstrated that the GSZ formula could improve sleep, with key ingredients likely being GABA, stearic acid, genistin, and coumestrol. The mechanism might involve the regulation of the Akt/GSK-3β pathway, as revealed by the network medicine analysis and experimental validation. Our current new findings shed light on the potential mechanisms underlying the sleep-enhancing effects of the GSZ formula, which could provide experimental evidence to develop innovative treatments for insomnia.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100107"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41517972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Benefits of Vernonia amygdalina in the Treatment of Inflammation and Its Associated Diseases","authors":"Du-Bois Asante ,&nbsp;Gideon Akuamoah Wiafe ,&nbsp;Korantema Mawuena Tsegah ,&nbsp;Nelson Kwabla Domey","doi":"10.1016/j.ccmp.2023.100122","DOIUrl":"10.1016/j.ccmp.2023.100122","url":null,"abstract":"<div><h3>Background</h3><p>In the presence of a stimulus that disrupts homeostasis, the body elicits a response known as inflammation. The inflammatory response is expected to restore the normal functioning of the body and initiate healing where necessary. However, the persistent activity of acute inflammation results in chronic inflammation which causes adverse consequences associated with diseases such as diabetes and arthritis. In traditional medical practice, <em>Vernonia amygdalina</em> is used to treat several ailments such as malaria, fever, diabetes, and inflammatory conditions such as those related to pain and swelling.</p></div><div><h3>Objective</h3><p>This review seeks to explore and discuss the therapeutic benefits of <em>V. amygdalina</em> in the treatment of inflammation and its associated diseases.</p></div><div><h3>Methods</h3><p>We reviewed the scientific literature indexed in PubMed, ScienceDirect and Springer, and discussed the reported anti-inflammatory properties of <em>V. amygdalina</em> and its curative activity in inflammation-associated diseases.</p></div><div><h3>Results</h3><p>A total of 30 published articles were identified describing the therapeutic effect of <em>V. amygdalina</em> in predominately experimental models. The anti-inflammatory properties of <em>V. amygdalina</em> have been linked to the presence of bioactive phytoconstituents predominantly present in the leaves of the plant.</p></div><div><h3>Conclusions</h3><p>Multiple studies have demonstrated that the aqueous and ethanolic extracts of <em>V. amygdalina</em> can inhibit the production of proinflammatory cytokines and enzymes in inflammatory cells, reducing inflammation and its associated complications. We critically appraise the current evidence, and discuss the potential use of this medicinal plant in the treatment of inflammatory conditions.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100122"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277237122300044X/pdfft?md5=d13fc61925d0578c34d556bf71082e49&pid=1-s2.0-S277237122300044X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139303756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviving Fertility: Phytochemicals as Natural Allies in the Fight against Non-genetic Male Infertility","authors":"Vivek P. Chavda ,&nbsp;Shreya S Sonak ,&nbsp;Pankti C. Balar ,&nbsp;Krupa Vyas ,&nbsp;Pranali Palandurkar ,&nbsp;Komal Mule ,&nbsp;Krishna Patel ,&nbsp;Vivek Hala ,&nbsp;Dixa A. Vaghela ,&nbsp;Devarshi Acharya ,&nbsp;Akta Vaishnav ,&nbsp;Jimil Gandhi ,&nbsp;Vasso Apostolopoulos","doi":"10.1016/j.ccmp.2024.100128","DOIUrl":"10.1016/j.ccmp.2024.100128","url":null,"abstract":"<div><h3>Background</h3><p>Male infertility poses a growing challenge to the healthcare system, with its prevalence on the rise. Unhealthy lifestyle, food, and addictions such as smoking, alcoholism, etc. accelerate the occurrence. While several approaches are being investigated to prevent and treat this condition, each therapeutic approach has its drawbacks. Traditional medications continue to play a crucial role in the healthcare system. In recent years, there has been a shift towards determining the efficacy of phytochemicals (or, herbal drugs) as remedies.</p></div><div><h3>Objective</h3><p>To evaluate the effect of various herbs, plant, metabolites, or a part of plant in management of non-genetic male infertility.</p></div><div><h3>Methods</h3><p>The male infertility-associated keywords were searched in PubMed, excluding those non-English writen papers. A total of 146 pertinent and closely connected records were included for full reading and inclusion in the systemic evaluation.</p></div><div><h3>Results</h3><p>The manuscript focuses on individual herbal drug components, their active ingredients, their role in improving the condition and quality of life, and decreasing the prevalence of male infertility.</p></div><div><h3>Conclusion</h3><p>Herbal medicinal plants show promising outcomes to treat male infertility. Herbal alternatives are appealing and have regained popularity. The future holds promise for some of these herbal treatments to advance with many showing improved outcomes in males with infertility issues.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100128"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371224000019/pdfft?md5=083ee448ca43e6b94fc8fdf1b3b9c542&pid=1-s2.0-S2772371224000019-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139632996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Xu's Influenza Decoction Combined with Oseltamivir on Influenza A: A Propensity Score Matching Study","authors":"Tianxi Chen ,&nbsp;Shuyan Fu ,&nbsp;Fengyuan Tian ,&nbsp;Qiushuang Li ,&nbsp;Hongyu Ling ,&nbsp;Yijie Lou ,&nbsp;Jun Tang ,&nbsp;Hong Zheng","doi":"10.1016/j.ccmp.2023.100113","DOIUrl":"10.1016/j.ccmp.2023.100113","url":null,"abstract":"<div><h3>Background</h3><p>The resurgence of seasonal influenza virus circulation has been seen in 2021–2022 after the temporary suppression in 2020–2021. Neuraminidase inhibitors (NAIs) are widely applied in the clinical treatment of influenza A despite several limitations.</p></div><div><h3>Objective</h3><p>To access the efficacy of Xu's influenza decoction (XID) in combination therapy with oseltamivir for the treatment of influenza A.</p></div><div><h3>Methods</h3><p>In this retrospective cohort study, the eligible participants were diagnosed with influenza A between June 1, 2018, and May 30, 2022, in the First Affiliated Hospital of Zhejiang Chinese Medical University. According to whether Xu's influenza decoction was applied, patients were divided into two groups: treated with or without XID. Propensity score matching (PSM) was used to further adjust the covariates between groups. The primary outcome was to compare time to defervescence via K-M curves, Breslow tests, and Cox regression analysis. In Cox proportional hazards model, a univariate analysis was performed to obtain preliminary results, while a further multivariate analysis was conducted to study the independent factors that influence defervescence. Subgroup analysis was conducted according to body temperature and time from onset to admission. The secondary outcome consisted of routine blood and C-reactive protein (CRP), length of stay, and medical costs.</p></div><div><h3>Results</h3><p>A total of 336 patients with influenza A were enrolled in this study (i.e., 163 patients in the XID+oseltamivir group; 173 patients in the oseltamivir group). After 1:1 matching via PSM, 230 patients meeting the criteria were included in the analysis, with 115 in each arm. The XID+oseltamivir group had shorter time to defervescence (36 h <em>vs</em> 44 h, <em>P</em> = 0.011), shorter length of stay (3 days <em>vs</em> 4 days, <em>P</em> = 0.018), and higher defervescence possibility (HR=1.384, 95%CI: 1.054–1.818). Subgroup analysis indicated that for patients during non-window period (≥ 48 h) with medium-grade fever (38.1℃–39℃), the XID+oseltamivir combination therapy reduced time to defervescence (<em>P</em> = 0.04995/0.004) with a higher defervescence possibility (HR=1.524/1.683). Meanwhile, there's no statistical significance but observable trends of the XID+oseltamivir group in the lower medical costs (3068.07 yuan <em>vs</em> 3120.68 yuan), the lower neutrophils% (48.53% <em>vs</em> 51.00%) and the higher lymphocyte% (39.83% <em>vs</em> 37.72%).</p></div><div><h3>Conclusion</h3><p>The combination of XID and oseltamivir can shorten the time to defervescence and length of stay in influenza A. Its antipyretic effect is mainly reflected in the medium-grade and non-window periods.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47324579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in vitro Anti-urolithiasis Activity of a Herbal Formulation: Spinacia oleracea L. and Coriandrum sativum L.","authors":"Sumathy Raj ,&nbsp;Monica Shree Gnana Soundara Rajan ,&nbsp;Suganthi Ramasamy ,&nbsp;Rimal Isaac Rajamony Suthies Goldy ,&nbsp;Ramathilaga Ariyamuthu ,&nbsp;Monisha Sudhagar ,&nbsp;Sakthivel Gandhi ,&nbsp;Prakash Shoba ,&nbsp;Manikandan Gurusamy","doi":"10.1016/j.ccmp.2023.100124","DOIUrl":"https://doi.org/10.1016/j.ccmp.2023.100124","url":null,"abstract":"<div><h3>Background</h3><p>Hard mineral and acid salt deposits that clump together in concentrated urine are known as kidney stones. A complicated chain of physicochemical events, including hyper saturation, nucleation, growth, aggregation, and retention in the urinary tract, leads to urolithiasis, the third most prevalent urinary tract issue. Numerous traditional medications and therapies are less successful in treating urolithiasis and frequently cause recurrences.</p></div><div><h3>Objective</h3><p>The present study is focused on evaluating the anti-urolithiasis activities of the polyherbal formulation prepared from <em>Spinacia oleracea</em> L<em>.</em> and <em>Coriandrum sativum</em> L. seeds.</p></div><div><h3>Methods</h3><p>To evaluating the anti-urolithiasis activities of the polyherbal formulation prepared from <em>S. oleracea</em> and <em>C. sativum</em> seeds against oxalate crystals by employing an <em>in vitro</em> inhibition, aggregation, and nucleation assay, employing a spectrophotometer followed by microscopic observation, and further monitored by egg semipermeable membrane model, using cystone as a standard drug.</p></div><div><h3>Results</h3><p>In the present work, the alkaloids, glycosides, flavonoids, glycosides, phenols, saponins, steroids, tannins, terpenoids, and triterpenoids were found in the extracts of <em>S. oleracea</em> and <em>C. sativum</em>. The <em>in vitro</em> assays in the present study clearly indicated that all the mixture extracts readily prevented crystal nucleation, growth and aggregation. The aqueous extract of the mixture inhibited significant inhibitory potential against the crystallization process.</p></div><div><h3>Conclusion</h3><p>It can be said that the phyto-constituents enriched homemade preparation possibly will be used as a medication for the management of kidney stones by dissolving the oxalate stone in the kidney.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100124"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371223000463/pdfft?md5=046c7ab340a7c93285826e0964d3a469&pid=1-s2.0-S2772371223000463-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139548979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diuretic Activity of Ethanol Extract of Piper attenuatum Leaves Might Be Due to the Inhibition of Carbonic Anhydrase Enzyme: An in vivo and in silico Investigation","authors":"Shikha Sharma ,&nbsp;Gaurav Kumar ,&nbsp;Nitin Kumar ,&nbsp;Neeraj K. Sethiya ,&nbsp;Dheeraj Bisht","doi":"10.1016/j.ccmp.2023.100117","DOIUrl":"https://doi.org/10.1016/j.ccmp.2023.100117","url":null,"abstract":"<div><h3>Background</h3><p>Ayurvedic system of medicine is well established for prevention and treatment of renal problems. There are vast number of medicinal plants mentioned in Ayurvedic system of medicine, including <em>Piper attenuatum</em> Buch.-Ham. ex Miq., are known to exhibits diuretic properties.</p></div><div><h3>Objective</h3><p>To investigate possible mechanism and diuretic activity of ethanol extract of <em>P. attenuatum</em> leaves.</p></div><div><h3>Methods</h3><p>Present study reports an <em>in vivo</em> diuretic activity of ethanol extracts of <em>P. attenuatum</em> leaves.Twenty-four Sprague-Dawley male rats were deprived from food and water for 20 h, followed by being divided into four groups to receive normal saline, Furosemide, and two doses of the ethanol extract, respectively. Further, the ethanol extract were subjected for prelimnary phytochemical screening test, and analytical investigation was performed via high-performance thin-layer chromatography (HPTLC), followed by effectiveness exploration of putative bioactive compounds from <em>P. attenuatum</em> against human carbonic anhydrase (hCA) enzyme using molecular docking tool.</p></div><div><h3>Results</h3><p>During phytochemicals screening, several groups of compounds such as amides, terpenoids, proteins, flavonoids, and glycosides have been identified. Further, HPTLC reveals presence of carbohydrates, proteins, amino acids, steroids, alkaloids, flavonoids, tannins, glycosides, and terpenoids. Significant diuretic action was revealed for both 200 mg/kg bw and 400 mg/kg bw of ethanol extract of <em>P. attenuatum</em> leaves. Interestingly, molecular docking analysis demonstrated greater binding affinity of compounds such as Cepharadione A, Norcepharadione B, Galbelgin, Crotepoxide, and Pipoxide chlorohydrin out of total 23 studied compounds against hCA isoforms (a key biomarker for diuretic) and score is comparable with standard drug Acetazolamide.</p></div><div><h3>Conclusion</h3><p>It was concluded from the present studies that ethanol extract of <em>P. attenuatum</em> leaves exhibits significant diuretic activity. The activity may be correlated due to the presence of Cepharadione A, Norcepharadione B, Galbelgin, Crotepoxide, and Pipoxide chlorohydrin as key component responsible for inhibition of hCA isoforms.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371223000396/pdfft?md5=39dda6e1c980d12247a9e0dd1b28636e&pid=1-s2.0-S2772371223000396-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92067973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Screening and Evaluation of Pharmacological Potential of Baliospermum solanifolium Leaf Extract","authors":"Amit Mazumder,&nbsp;Rabindra Nath Acharyya,&nbsp;Milton Kumar Kundu,&nbsp;Pritam Kundu,&nbsp;Md. Amirul Islam,&nbsp;Md. Mustafizur Rahman","doi":"10.1016/j.ccmp.2023.100123","DOIUrl":"10.1016/j.ccmp.2023.100123","url":null,"abstract":"<div><h3>Background</h3><p><em>Baliospermum solanifolium</em> is a traditional medicinal plant, locally known as “dantigaacha” from Euphorbiaceae family, and has been used in different diseases treatments likes helminthiasis, skin diseases, diabetes, snake-bite, and leukoderma.</p></div><div><h3>Objectives</h3><p>Depending on the traditional uses of <em>B. solanifolium</em> and the lack of scientific works<em>,</em> we aimed to conduct some pharmacological tests on its leaves.</p></div><div><h3>Methods</h3><p>Active constituents of <em>B. solanifolium</em> were assessed by qualitative screening. DPPH (2,2-diphenyl-1-picrylhydrazyl) was used for free radical scavenging assay. Behavioral change and mortality rate were used as indicators to assess the toxicity of <em>B. solanifolium</em>. The antidiabetic potential was evaluated by oral glucose tolerance test (OGTT) and alpha-glucosidase enzyme inhibitory activity. In antidiarrheal activity, castor oil-induced mice were used. Antibacterial test was carried out by disk diffusion assay and MIC determination. Furthermore, analgesic activity was done by acetic acid-induced writhing method.</p></div><div><h3>Results</h3><p><em>B. solanifolium</em> contains alkaloids, carbohydrates, glycosides, flavonoids, tannis, phenolics etc. Acute toxicity test ensured the safety of the extract. In DPPH, the extract showed antioxidant activity with an IC₅₀ (concentration needed to scavenge 50% DPPH) value of 61.65 µg/mL. Total phenolic, flavonoid and tannin contents were determined as 124 mg GAE/g, 440 mg QE/g and 344 mg GAE/g dried extract, respectively. In OGTT, the extract reduced the blood glucose level significantly. In the alpha-glucosidase inhibitory activity, the plant showed a concentration-dependent reduction of the enzyme at IC₅₀ of 8.53 mg/mL. In the antidiarrheal test, <em>B. solanifolium</em> inhibit defecation 30.69% and 53.41% at dose 250 mg/kg and 500 mg/kg, respectively. The extract also showed antibacterial activity against four bacterial strains in the disk diffusion method and minimum inhibitory concentration (MIC) values were 62.5 µg/mL, 62.5 µg/mL, 125 µg/mL and 62.5 µg/mL against four bacterial strains, independently. In analgesic activity test, the extract reduced the writhing impulse up to 48.51% at 500 mg/kg dose.</p></div><div><h3>Conclusion</h3><p><em>B. solanifolium</em> contains many active phytoconstituents which may be the possible candidates for targeting diabetes, microbial infection, diarrhea and pain in the future.</p></div>","PeriodicalId":72608,"journal":{"name":"Clinical complementary medicine and pharmacology","volume":"4 1","pages":"Article 100123"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772371223000451/pdfft?md5=0e5098eb8f5de5fafb318b7a58dbc7fb&pid=1-s2.0-S2772371223000451-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139292065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}