Engineered regeneration最新文献_第3页

Mapping the trajectory of organoid research: Insights into complex models and advanced technologies 绘制类器官研究的轨迹：对复杂模型和先进技术的见解

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.11.003

Yiming Zhang , Lianyong Wang , Qingqian Zhao , Jinhao Gong , Ye Tian , Yang Liu , Shanfeng Chen , Xin Zhao , Qiang Yang , Yanhong Zhao

In recent years, organoid technology has become a pivotal advancement in stem cell research, offering an unprecedented experimental platform that mimics the morphology and function of human organs, pushing modern life sciences to the forefront. This technology has successfully developed complex three-dimensional (3D) structures derived from human pluripotent or adult stem cells, closely resembling natural organs in tissue and function. Organoid models facilitate in-depth studies of organ development, disease progression, and drug interactions, effectively addressing ethical and practical limitations in traditional biomedical research. Despite its promising development, organoid technology faces challenges in practical applications, particularly in constructing complex organs like the brain, heart, and bone tissue. Therefore, we conducted a bibliometric analysis of 8051 publications on organoids from the Web of Science Core Collection (WoSCC) database (2000-2025). These studies were categorized into five clusters related to current situations and future application directions. Based on our bibliometric analysis, systematically summarize the development of organoid technologies, from laboratory to preclinical applications, exploration of complex organoids, and advanced technologies, while analyzing their limitations and prospects. This review will provide an objective perspective to identify current trends in organoid research and provide new insights into the future research and clinical applications of organoids by teasing out the past-present of organoid research.

近年来，类器官技术已成为干细胞研究的关键进展，提供了一个前所未有的模拟人体器官形态和功能的实验平台，将现代生命科学推向了前沿。这项技术已经成功地从人类多能干细胞或成体干细胞中开发出复杂的三维（3D）结构，在组织和功能上与自然器官非常相似。类器官模型促进了器官发育、疾病进展和药物相互作用的深入研究，有效地解决了传统生物医学研究中的伦理和实践限制。尽管发展前景广阔，但类器官技术在实际应用中仍面临挑战，特别是在构建复杂器官如大脑、心脏和骨组织方面。因此，我们对Web of Science Core Collection （WoSCC）数据库（2000-2025）中8051篇关于类器官的出版物进行了文献计量学分析。根据研究现状和未来应用方向，将这些研究分为五大类。在文献计量学分析的基础上，系统总结了类器官技术的发展，从实验室到临床前应用，复杂类器官的探索和先进技术，同时分析了它们的局限性和前景。本文旨在通过梳理类器官研究的过去和现在，为识别类器官研究的当前趋势提供一个客观的视角，并为未来的研究和临床应用提供新的见解。

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引用次数: 0

Ultrasonic manipulation in tissue engineering 组织工程中的超声操作

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.03.001

Zijun Guan, Jianbo Huang, Yang Gao, Hongju Zhou, Liyun Wang, Lang Ma, Li Qiu

Ultrasonic waves exert radiation force on cells and other cell size particles, applied in particle manipulation, growth factor delivery, substance cavitation, and single cell tweezing. Featuring in the safe, contactless, precise, and tunable properties and advantages, ultrasonic waves can be used to control cell's locations aiding in the morphogenesis of complex cell systems, which will be widely used in the future generation tissue engineering. In this review, we summarized current application of ultrasonic waves in the field of cell manipulation and tissue engineering. First, we briefly introduced the physical mechanisms of cell manipulation and described the five kinds of device designs including holographic device, tweezer device, stream standing wave device, surface acoustic wave device, and bulk acoustic wave device. Secondly, we concluded recent works to culture tissue cells in certain spatial patterns using ultrasonic device including bone tissue, cartilage, cardiac muscle, skeletal muscle, endothelial, and neurons. Finally, we systematically highlighted the current challenges and future perspectives. It is believed that this cutting review will substantially stimulate the development and widespread utilization of ultrasonic standing wave in future tissue engineering applications.

超声波对细胞和其他细胞大小的粒子施加辐射力，应用于粒子操纵、生长因子传递、物质空化和单细胞镊子。超声波具有安全、无接触、精确、可调等特点，可用于控制细胞的位置，有助于复杂细胞系统的形态发生，将在下一代组织工程中得到广泛应用。本文就超声技术在细胞操作和组织工程领域的应用现状进行综述。首先，我们简要介绍了细胞操纵的物理机制，并描述了全息装置、镊子装置、流驻波装置、表面声波装置和体声波装置五种装置的设计。其次，总结了近年来利用超声装置培养组织细胞的研究成果，包括骨组织、软骨、心肌、骨骼肌、内皮细胞和神经元。最后，我们系统地强调了当前的挑战和未来的展望。相信这一综述将极大地促进超声驻波在未来组织工程中的发展和广泛应用。

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引用次数: 0

Current advances and prospects in biomaterials-guided tools for liver organoids research 生物材料导向类肝器官研究工具的现状与展望

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.07.004

Sana Ahmed , Eman Alshehri , Sarah Nazneen , Fayrouz Attia , Dalia Obeid , Hanan Almuzaini , Alaa Alzahrani , Jahan Salma , Iriya Fujitsuka , Abdullah M. Assiri , Dieter C. Broering , Raja Chinnappan , Ahmed Yaqinuddin , Tanveer Ahmad Mir

Liver is the largest solid organ in the human body engaged in an array of critical physiological activities that primarily support metabolism, digestion, nutrient storage, detoxification. Liver dysfunction due to disease or surgical intervention often leads to severe life-threatening complications or death in humans. Therefore, in vitro liver models that mimic key functional characteristics are considered a reliable option for the study of liver diseases and the development of new therapeutic agents. Furthermore, they can overcome the limitations of conventional monolayer cultures and animal related experiments in assessing the response of new therapeutic agents and drug molecules. In recent years, the emergence and advancement of organoid technology has greatly facilitated the development of reliable in vitro liver models for a variety of biomedical and pharmacological applications. However, organoid culture primarily relies on tumor-derived extracellular matrix, such as Matrigel, which pose challenges due to its xenogeneic nature and variable composition. Therefore, creating organoid models using Matrigel-free hydrogel materials could significantly improve the outcomes of regenerative medicine and experimental studies. In this review, we provide an overview of rapidly evolving biomaterials for organoid research. We then outline preparation methods and the most relevant studies applying different hydrogels for engineering liver organoid models. Finally, we discuss the challenges, future perspectives, and opportunities of hydrogels in engineering next-generation liver organoid models for translational applications.

肝脏是人体最大的固体器官，参与一系列重要的生理活动，主要是支持新陈代谢、消化、营养储存和解毒。由于疾病或手术干预引起的肝功能障碍往往导致严重的危及生命的并发症或死亡。因此，模拟关键功能特征的体外肝脏模型被认为是肝脏疾病研究和新治疗剂开发的可靠选择。此外，它们可以克服传统单层培养和动物相关实验在评估新治疗剂和药物分子反应方面的局限性。近年来，类器官技术的出现和进步极大地促进了可靠的体外肝脏模型的发展，用于各种生物医学和药理学应用。然而，类器官培养主要依赖于肿瘤来源的细胞外基质，如Matrigel，由于其异种性和可变成分，这带来了挑战。因此，使用不含matrigel的水凝胶材料创建类器官模型可以显著改善再生医学和实验研究的结果。在这篇综述中，我们提供了快速发展的生物材料类器官的研究综述。然后，我们概述了制备方法和应用不同水凝胶用于工程肝类器官模型的最相关研究。最后，我们讨论了水凝胶在工程下一代肝类器官模型转化应用中的挑战、未来前景和机遇。

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引用次数: 0

MicroRNAs and mRNAs: A comparative analysis in translation and commercialization MicroRNAs和mrna：翻译和商业化的比较分析

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.04.001

Jamie Cheng , Wubin Bai , Thomas Caranasos

Success with mRNA vaccines has positioned microRNAs as possibly the nextgeneration therapeutics. While mRNAs have demonstrated rapid translation into effective vaccines, microRNAs possess certain advantages in gene regulation, particularly in targeting multiple genes concurrently. Therefore, they are promising for the treatment of cancer and cardiovascular disorders. This paper provides information about microRNAs with regard to their therapeutic applications, based on their stability, tissue distribution, and specific gene modulation. However, commercialization of microRNA also faces challenges, especially in aspects of delivery and regulatory approval. Unlike the mRNAs, which benefit from the delivery method of lipid nanoparticles, the microRNAs need an innovative delivery method that guarantees target efficacy and ensures minimal off-target effects. Additionally, regulatory pathways for microRNAs have not been fully developed and have to be well-assessed for their safety and effectiveness. We propose that microRNAs and mRNAs may exert complementary therapeutic functions, as mRNAs in vaccines are superior while microRNAs offer new roads to treatments in multifactorial diseases. Since these challenges are being overcome, microRNA-based therapies have the potential to revolutionize treatments for gene regulation and thereby affect the future of RNA therapeutics. This paper outlines the therapeutic potential of microRNAs and the steps involved in translation into clinical application.

mRNA疫苗的成功使microrna有可能成为下一代疗法。虽然mrna已被证明可以快速转化为有效的疫苗，但microrna在基因调控方面具有一定优势，特别是在同时靶向多个基因方面。因此，它们有望用于癌症和心血管疾病的治疗。本文提供了关于microrna的信息，关于他们的治疗应用，基于他们的稳定性，组织分布，和特定的基因调节。然而，microRNA的商业化也面临着挑战，特别是在递送和监管审批方面。与受益于脂质纳米颗粒递送方法的mrna不同，microrna需要一种创新的递送方法，以保证靶向效果并确保最小的脱靶效应。此外，microrna的调控途径尚未完全开发，必须对其安全性和有效性进行充分评估。我们提出microRNAs和mrna可能发挥互补的治疗功能，因为mrna在疫苗中具有优势，而microRNAs为多因子疾病的治疗提供了新的途径。由于这些挑战正在被克服，基于微RNA的治疗有可能彻底改变基因调控的治疗方法，从而影响RNA治疗的未来。本文概述了microrna的治疗潜力和转化为临床应用所涉及的步骤。

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引用次数: 0

Immunomodulating red blood cell coating for mitigation of foreign body reactions 免疫调节红血球涂层减轻异物反应

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.09.001

Yi Ye , Jinyun Wu , Tao Sun , Minghong Huang , Jiaping Pan , Danjing Hu , Keying Chen , Dawei Wang , Chenxi Wang , Yangkun Shen , Qi Chen , Baolin Huang

Chronic inflammation of biomedical implants usually leads to fibrosis and device malfunction in the long term. To address these issues, a cell-crosslinked coating of red blood cells (RBCs) was developed to imitate a self-friendly biological membrane and camouflage the implants from immune system. Using the widely applied poly(dimethylsiloxane) (PDMS) as a model substrate, a natural polymer hyaluronic acid (HA) layer was constructed upon PDMS (PDMS-HA), which was further decorated with RBCs (PDMS-HA-RBC). Compared to pristine PDMS, both PDMS-HA and PDMS-HA-RBC notably polarized the original macrophages into an anti-inflammatory phenotype (M2) rather than a pro-inflammatory phenotype (M1). Especially, PDMS-HA-RBC exhibited the highest M2/M1 ratios of macrophages, suggesting efficient modulation effects of inflammation reactions by the RBCs coating. Moreover, in vivo results found that PDMS induced considerable foreign body reactions (FBRs) and extensive fibrosis formation. In contrast, PDMS-HA revealed a significantly thinner fibrotic layer while PDMS-HA-RBC induced the least amount of fibrosis. In addition, PDMS-HA-RBC exhibited the highest fluorescent intensity of CD206 (M2 antigen) and the lowest fluorescent intensity of CD86 (M1 antigen). It was speculated that the RBCs coating-mediated macrophage polarization was mainly attributed to the presence of immune escape antigens (such as CD47 and CD59) upon the cell coating. Altogether, our living RBCs coating demonstrated significant potentials in mitigating FBRs of PDMS, indicating their promising applications in surface engineering of various biomedical implants.

生物医学植入物的慢性炎症通常会导致纤维化和设备长期故障。为了解决这些问题，一种细胞交联的红细胞（rbc）涂层被开发出来，以模仿一种自友好的生物膜，并保护植入物免受免疫系统的攻击。以应用广泛的聚二甲基硅氧烷（PDMS）为模型底物，在PDMS （PDMS-HA）上构建天然聚合物透明质酸（HA）层，并在其表面进行红细胞修饰（PDMS-HA- rbc）。与原始PDMS相比，PDMS- ha和PDMS- ha - rbc均明显使原始巨噬细胞极化为抗炎表型（M2）而不是促炎表型（M1）。特别是PDMS-HA-RBC巨噬细胞M2/M1比值最高，提示红细胞包被对炎症反应有有效的调节作用。此外，体内实验结果发现，PDMS诱导了大量的异物反应（FBRs）和广泛的纤维化形成。相比之下，PDMS-HA显示纤维化层明显变薄，而PDMS-HA- rbc诱导的纤维化量最少。此外，PDMS-HA-RBC对M2抗原CD206的荧光强度最高，对M1抗原CD86的荧光强度最低。推测红细胞包被介导的巨噬细胞极化主要是由于细胞包被上存在免疫逃逸抗原（如CD47和CD59）。总之，我们的活红细胞涂层在减轻PDMS的fbr方面显示出显著的潜力，表明它们在各种生物医学植入物的表面工程中具有广阔的应用前景。

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引用次数: 0

The microenvironment in atherosclerosis: molecular regulation mechanism and immunotherapy 动脉粥样硬化中的微环境：分子调控机制和免疫治疗

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.07.002

Xiaoyu Teng , Qinlian Jiao , Yidan Ren , Xin Su , Zigan Li , Yuxuan Cai , Tangbin Hu , Maoxiao Feng , Xiaoyan Liu , Ming Xia , Jun Tai , Yana Zhang , Yunshan Wang , Mo Wang

Atherosclerosis is a chronic inflammatory disease closely linked to immune dysregulation. The immune microenvironment within atherosclerotic lesions is highly complex, involving diverse innate and adaptive immune cells and their intricate crosstalk. These immune interactions collectively contribute to plaque formation, progression, and destabilization. This review comprehensively examines the roles of key immune cell populations—including macrophages, dendritic cells (DCs), neutrophils, mast cells, natural killer (NK) cells, T cells, and B cells—in regulating inflammation, foam cell formation, and lesion stability. Special attention is given to intercellular regulatory circuits such as the Th1–M1 feedback loop, the OX40L–Th17 axis, and DC–T–NK amplification loops. Furthermore, the review highlights the influence of immunometabolic reprogramming on immune cell function and plaque phenotype, illustrating how metabolic states shape inflammatory outcomes. It also discusses the contribution of key signaling pathways—including Toll-like receptors (TLRs), the NOD-like receptor protein 3 (NLRP3) inflammasome, and proprotein convertase subtilisin/kexin type 9 (PCSK9)—to atherosclerotic inflammation and plaque vulnerability. Advances in immunotherapy are also reviewed, including anti-inflammatory agents such as colchicine and canakinumab, as well as emerging vaccine strategies targeting lipid metabolism and vascular inflammation.

A deeper understanding of immune cell interplay and signaling dynamics in atherosclerosis will provide a foundation for developing more effective, multi-targeted immunotherapeutic interventions. Future research should aim to refine these strategies to maximize efficacy and safety, with the goal of reducing the global burden of atherosclerotic cardiovascular disease.

动脉粥样硬化是一种与免疫失调密切相关的慢性炎症性疾病。动脉粥样硬化病变内的免疫微环境是高度复杂的，涉及多种先天和适应性免疫细胞及其复杂的相互作用。这些免疫相互作用共同促进斑块的形成、进展和不稳定。本文综述了包括巨噬细胞、树突状细胞（dc）、中性粒细胞、肥大细胞、自然杀伤细胞（NK）、T细胞和B细胞在内的关键免疫细胞群在调节炎症、泡沫细胞形成和病变稳定性中的作用。特别关注细胞间调节回路，如Th1-M1反馈回路、OX40L-Th17轴和DC-T-NK放大回路。此外，该综述强调了免疫代谢重编程对免疫细胞功能和斑块表型的影响，说明了代谢状态如何影响炎症结果。它还讨论了关键信号通路的贡献，包括toll样受体（TLRs）， nod样受体蛋白3 （NLRP3）炎症小体和蛋白转化酶枯草杆菌素/酮蛋白9 （PCSK9）对动脉粥样硬化炎症和斑块易感性的影响。免疫治疗的进展也进行了回顾，包括抗炎药，如秋水仙碱和canakinumab，以及针对脂质代谢和血管炎症的新兴疫苗策略。对动脉粥样硬化中免疫细胞相互作用和信号动力学的深入了解将为开发更有效的多靶向免疫治疗干预提供基础。未来的研究应旨在完善这些策略，以最大限度地提高疗效和安全性，以减少动脉粥样硬化性心血管疾病的全球负担。

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引用次数: 0

Corrigendum to ‘LSM6 promotes cell proliferation and migration regulated by HMGB1 in laryngeal squamous cell carcinoma’[Engineered Regeneration 5 (2024) 247-254] “喉鳞癌中LSM6促进HMGB1调控的细胞增殖和迁移”的更正[工程再生5 (2024)247-254]

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.12.002

Dengbin Ma , Jiayi Li , Hui Li , Yao Tang , Xia Gao , Hong Chen , Xiaoyun Qian , Xiaohui Shen

引用次数: 0

Bioactive diamond scaffolds support neuronal survival and axonal growth 生物活性金刚石支架支持神经元存活和轴突生长

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.07.001

R J F Sørensen , Nicolas Bertram , Ugne Dubonyte , Bob A Hersbach , Alison Salvador , Anpan Han , Agnete Kirkeby , Rune W Berg , Jaspreet Kaur

Injury to the central nervous system (CNS) can have devastating consequences for the individual, and strategies to promote endogenous axonal regeneration may be a promising future therapeutic avenue. In the case of spinal cord injury, one approach is to generate a scaffold-bridge across the injury site, through which the neuronal axons can grow and reconnect. Inspired by the various properties of diamond, including its chemical inertness, we proposed a strategy in which synthetic diamond scaffolds were coated with proteins with beneficial properties to promote biocompatibility of the scaffolds towards neurons. Here, we demonstrated that bare, non-coated diamond scaffolds, when terminated with either oxygen or hydrogen, were unable to adhere to the human embryonic stem cell-derived interneurons in culture. In contrast, oxygen terminated protein-coated scaffolds (i.e., bioactive diamond scaffold) efficiently enabled neuronal attachment and supported the survival, migration, and neurite elongation across an induced injury gap in culture. Further, hydrogen terminated bioactive scaffolds also promoted cell adhesion, migration, and neurite elongation upon injury, but not as efficiently as oxygen-terminated bioactive scaffolds. With this data we suggest that bioactive synthetic diamond scaffolds could provide a valuable tool for future therapeutic strategies in the context of CNS injuries.

中枢神经系统（CNS）损伤对个体具有毁灭性的后果，促进内源性轴突再生的策略可能是未来有希望的治疗途径。在脊髓损伤的情况下，一种方法是在损伤部位产生一个支架桥，通过它神经元轴突可以生长和重新连接。受金刚石的各种特性（包括其化学惰性）的启发，我们提出了一种策略，在合成金刚石支架上涂覆具有有益特性的蛋白质，以促进支架对神经元的生物相容性。在这里，我们证明了裸的、无涂层的金刚石支架，当用氧或氢终止时，不能附着在培养的人类胚胎干细胞衍生的中间神经元上。相比之下，氧终止蛋白包被支架（即生物活性金刚石支架）有效地使神经元附着，并支持在培养中诱导损伤间隙中的存活、迁移和神经突伸长。此外，氢端生物活性支架在损伤后也能促进细胞粘附、迁移和神经突伸长，但效果不如氧端生物活性支架。根据这些数据，我们认为生物活性合成金刚石支架可以为未来中枢神经系统损伤的治疗策略提供有价值的工具。

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引用次数: 0

Bioactive diamond scaffolds support neuronal survival and axonal growth 生物活性金刚石支架支持神经元存活和轴突生长

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.07.001

R J F Sørensen , Nicolas Bertram , Ugne Dubonyte , Bob A Hersbach , Alison Salvador , Anpan Han , Agnete Kirkeby , Rune W Berg , Jaspreet Kaur

Injury to the central nervous system (CNS) can have devastating consequences for the individual, and strategies to promote endogenous axonal regeneration may be a promising future therapeutic avenue. In the case of spinal cord injury, one approach is to generate a scaffold-bridge across the injury site, through which the neuronal axons can grow and reconnect. Inspired by the various properties of diamond, including its chemical inertness, we proposed a strategy in which synthetic diamond scaffolds were coated with proteins with beneficial properties to promote biocompatibility of the scaffolds towards neurons. Here, we demonstrated that bare, non-coated diamond scaffolds, when terminated with either oxygen or hydrogen, were unable to adhere to the human embryonic stem cell-derived interneurons in culture. In contrast, oxygen terminated protein-coated scaffolds (i.e., bioactive diamond scaffold) efficiently enabled neuronal attachment and supported the survival, migration, and neurite elongation across an induced injury gap in culture. Further, hydrogen terminated bioactive scaffolds also promoted cell adhesion, migration, and neurite elongation upon injury, but not as efficiently as oxygen-terminated bioactive scaffolds. With this data we suggest that bioactive synthetic diamond scaffolds could provide a valuable tool for future therapeutic strategies in the context of CNS injuries.

中枢神经系统（CNS）损伤对个体具有毁灭性的后果，促进内源性轴突再生的策略可能是未来有希望的治疗途径。在脊髓损伤的情况下，一种方法是在损伤部位产生一个支架桥，通过它神经元轴突可以生长和重新连接。受金刚石的各种特性（包括其化学惰性）的启发，我们提出了一种策略，在合成金刚石支架上涂覆具有有益特性的蛋白质，以促进支架对神经元的生物相容性。在这里，我们证明了裸的、无涂层的金刚石支架，当用氧或氢终止时，不能附着在培养的人类胚胎干细胞衍生的中间神经元上。相比之下，氧终止蛋白包被支架（即生物活性金刚石支架）有效地使神经元附着，并支持在培养中诱导损伤间隙中的存活、迁移和神经突伸长。此外，氢端生物活性支架在损伤后也能促进细胞粘附、迁移和神经突伸长，但效果不如氧端生物活性支架。根据这些数据，我们认为生物活性合成金刚石支架可以为未来中枢神经系统损伤的治疗策略提供有价值的工具。

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引用次数: 0

Transcript-activated collagen matrix for enhanced bone marrow stem cell differentiation and osteochondral repair 转录活化胶原基质增强骨髓干细胞分化和骨软骨修复

Q1 Medicine

Engineered regeneration

Pub Date : 2025-01-01 DOI: 10.1016/j.engreg.2025.03.002

Gang Zhong , Yixuan Luo , Meng Wang , Zhengran Yu , Xuenong Zou , Gang Wang , Fei Chen , Yin Yu

The regeneration of critical-sized osteochondral defects remains a significant challenge due to the limited self-healing capacity of cartilage. Traditional approaches, such as autologous chondrocyte implantation (ACI) and matrix-induced autologous chondrocyte implantation (MACI), have shown promise but are limited by issues like insufficient cell availability, dedifferentiation of chondrocytes during expansion, and the formation of fibrocartilage rather than functional hyaline cartilage. This study presents a promising approach utilizing transcript-activated matrices (TAMs) with mRNA to enhance the therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) in situ. Chemically modified mRNA (cmRNA) encoding transforming growth factor β3 (TGF-β3) was encapsulated in a collagen hydrogel to provide localized, sustained delivery of chondrogenic signals. In a rat model of critical-sized osteochondral defects, this strategy significantly promoted cartilage regeneration, achieving structural and molecular restoration within six weeks. Histological and biochemical analyses revealed robust chondrogenesis, enhanced extracellular matrix deposition, and superior mechanical properties. Moreover, TAM therapy maintained subchondral bone integrity This work highlights the transformative potential of mRNA-activated matrices as a platform technology that not only addresses key limitations of existing cartilage repair strategies but also provides a biomimetic microenvironment that guides stem cell differentiation and tissue regeneration.

由于软骨的自愈能力有限，骨软骨缺损的再生仍然是一个重大的挑战。传统的方法，如自体软骨细胞植入（ACI）和基质诱导的自体软骨细胞植入（MACI），已经显示出前景，但受到诸如细胞可用性不足、软骨细胞在扩张过程中去分化以及形成纤维软骨而不是功能透明软骨等问题的限制。本研究提出了一种利用mRNA转录激活基质（TAMs）原位增强骨髓间充质干细胞（BMSCs）治疗潜力的有希望的方法。将编码转化生长因子β3 （TGF-β3）的化学修饰mRNA （cmRNA）包裹在胶原水凝胶中，以提供局部、持续的软骨生成信号传递。在大鼠骨软骨缺损模型中，该策略显著促进了软骨再生，在6周内实现了结构和分子的修复。组织学和生化分析显示强健的软骨形成，增强的细胞外基质沉积和优越的力学性能。此外，TAM治疗保持了软骨下骨的完整性。这项工作强调了mrna激活基质作为平台技术的转化潜力，它不仅解决了现有软骨修复策略的关键限制，而且提供了一个引导干细胞分化和组织再生的仿生微环境。

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引用次数: 0