FEMS microbes最新文献

The rise of antibiotic-resistant bacteria is a critical issue across various ecological interfaces, highlighting the need for a One Health approach. Enterococcus spp., known for their ability to acquire and disseminate resistance, serve as an excellent model due to their presence in diverse hosts and environments. This study investigates antimicrobial resistance, biofilm formation capacity, and the efficacy of antibiotics on biofilm biomass reduction in isolates from multiple sources. A total of 197 Enterococcus isolates were used. Antimicrobial resistance was determined using the Kirby-Bauer disc diffusion method, and minimum inhibitory concentrations were tested against vancomycin, tetracycline, and ampicillin. Biofilm formation capacity was assessed, and 10 biofilm-formers were subjected to minimum biofilm inhibitory concentration (MBIC) tests to evaluate biofilm biomass reduction. The results showed high resistance rates to erythromycin (84.5%), ciprofloxacin (59.4%), and tetracycline (44.4%), with moderate resistance to ampicillin (36.2%), chloramphenicol (28%), and vancomycin (24.7%). Biofilm formation was observed in 65% of the isolates, with Enterococcus hirae producing the most biofilm biomass. Vancomycin and ampicillin were more effective in reducing biofilm biomass than tetracycline. Ampicillin-resistant isolates produced more biofilm, suggesting a link between resistance and biofilm formation. This study highlights the complexity of antibiotic-resistant Enterococcus spp. and their biofilms, emphasizing the need for research on One Health.

Antarctica is a very cold, isolated continent surrounded by frozen seas, yet these extreme environmental conditions have not restricted life and diversity in the sea. The marine environment is seasonally highly productive and harbours diverse and abundant communities of organisms, with many endemic species occurring nowhere else in the world. Such communities and their associated microbiomes are increasingly recognized as an unexplored source of novel antimicrobial products. Hence, the major aim of this study was to examine the antimicrobial potential of bacteria cultured from eight Antarctic marine invertebrate species, while gathering data on Antarctic microbial thermal and salinity tolerances. All cultured bacterial species (n = 34) were related to known psychrotrophs, with thermal tolerances that far exceeded those of their invertebrate hosts. Of note, two strains of Psychrobacter and Pseudomonas produced antagonistic activity towards epidemic methicillin-resistant Staphylococcus aureus, Micrococcus luteus, and Candida albicans in preliminary simultaneous antagonism screens. Draft whole genome sequence analysis revealed the presence of 13 biosynthetic gene clusters; including those with potential to produce betalactones, post-translationally modified peptide products, and arylpropynes. These results emphasize the need for more extensive and systematic surveys to identify novel biomolecules from Antarctic marine bacteria that may be exploited for societal gain.

Toxoplasmosis is a parasitic infection with significant implications for human health, particularly in its ocular form, which can lead to severe visual impairment. While both basic and clinical research have made considerable strides in understanding the biology and treatment of this parasite, challenges remain. Recent advancements in diagnostics, mainly through multimodal imaging, have improved the identification of active disease and predicting outcomes. Experimental therapies are also emerging, offering new hope for more effective treatments. However, the most critical insight from recent research, particularly emphasized at the 17th International Congress on Toxoplasmosis, is the necessity of a collaborative approach. Integrating basic and clinical research is essential for translating molecular and pathophysiological findings into effective clinical practices. This synergy is crucial for advancing treatment strategies and improving patient outcomes in ocular toxoplasmosis.

Toxoplasma gondii is an extremely "successful" opportunistic parasite that infects most warm-blooded animals, including humans. While the infection is generally largely asymptomatic, the infection of the eye presenting as ocular toxoplasmosis (OT) is a potentially blinding consequence. OT remains the most common cause of infectious retinochoroiditis and places a considerable socio-economic burden on societies, particularly in developing countries. Age is one of several factors influencing the clinical presentation and outcomes of OT. Older patients often exhibit more severe disease manifestations, larger retinal lesions, and poorer visual outcomes compared to younger individuals. This disparity is attributed to immunosenescence, the age-related decline in immune function, which impairs the body's ability to control the infection effectively. Consequently, older individuals are at a higher risk of severe complications and recurrent episodes of inflammation. Understanding the interplay between age and immune response is essential for developing targeted treatment strategies and improving patient outcomes in OT.

Avian influenza virus (AIV) poses significant threats to poultry and human health. This study investigates the impact of H9N2 AIV infection on the respiratory microbiota of chickens using 16S rRNA gene sequencing. Total 48 one-day-old specific pathogen-free chickens were assigned to six groups: a control and five post-infection groups (days 1, 3, 5, 7, and 9). After a 15-day microbiota stabilization period, the infected chickens received a viral inoculum (10⁷ TCID50/ml) via ocular, intra-nasal, and intra-tracheal routes. Tracheal and broncho-alveolar lavage samples were analyzed. Significant reductions in microbiota diversity were observed on days 5, 7, and 9 post-infection, compared to d0 controls. Permutational Multivariate Analysis of Variance confirmed significant beta diversity differences (P = 0.001) between infected and uninfected groups. The microbial shifts from d5 to d9 were marked by increased Proteobacteria, decreased Actinobacteria and Firmicutes, and a rise in Dickeya. Elevated type-I interferon (IFN-β) and viperin gene expression at d5 coincided with reduced microbiota diversity, highlighting the respiratory microbiota's role in modulating host responses to AIV H9N2 infection and suggesting potential biomarkers for respiratory dysbiosis.

Bacteriological agar plates are commonly used to carry out experiments for the selective growth of microorganisms and the isolation of single-strain colonies. However, the presence of agar itself may be a confounding factor since it may serve as a source of carbon and energy. Moreover, there have been ongoing constraints on the production and sourcing of agar. These concerns have led to an interest in the development of agar substitutes. Silica hydrogels are entirely inorganic carbon-free polymeric materials that lack any source of micronutrients. Herein, a revised method for the preparation of silica hydrogels as a solid culture medium is reported. These gels can be formulated with a range of nutrient-rich or minimal media supplemented with various carbon sources, and can be manipulated in the same manner as agar gels. Their use for the culture and isolation of diverse microorganisms, including both Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi is demonstrated. These silica hydrogels supplemented with either antibiotics or other molecules of interest can also be used for microbial selection experiments.

The apicomplexan parasite Toxoplasma gondii has a complex life cycle. Access to sexual stages and sporozoite-containing oocysts, essential for studying the parasite's environmental transmission, is limited and requires animal experiments with cats. Thus, alternatives and resource-efficient methods are needed. Several molecular factors and transcriptional switches responsible for differentiation have been identified in recent years. In tachyzoites, drug-induced inhibition of the histone deacetylase HDAC3, or genetic depletion of transcription factors regulating HDAC3, leads to the expression of genes that are specific to sexual stages and oocysts. Here, we applied this concept and showed that the commercially available HDAC3 inhibitor apicidin could be used to identify the hitherto unknown antigen of the sporozoite-specific monoclonal antibody G1/19 in tachyzoites. Using mass spectrometry of immunoprecipitated G1/19 target protein from apicidin-treated cultures, we identified it as SporoSAG. In addition, for the much less abundant sporozoite-specific protein LEA860, apicidin treatment was still sufficient to induce a detectable protein level in immunofluorescence microscopy. We also discuss further applications and the limitations of this approach. This allows to overcome issues with the paucity of material of sexual stages and oocysts from T. gondii to some extent without the need for cat-derived material.

Anaerobic gut fungi (AGF, Neocallimastigomycota) inhabit the alimentary tract of herbivores. Although strict anaerobes, studies have suggested their capacity to retain viability after various durations of air exposure. It is currently unclear whether AGF can actively grow, and not merely survive, in redox potentials (E_h) higher than those encountered in the herbivorous gut. We evaluated the growth of two AGF strains (Orpinomyces joyonii and Testudinimyces gracilis) at various E_h levels, achieved by manipulating the concentrations of reductant (cysteine hydrochloride) in culture media. Both strains exhibited robust and sustainable growth at negative E_h (-50 mV or below). However, growth in the absence of cysteine hydrochloride (E_h value around +50 mV) was possible only for O. joyonii and only for one subcultivation. The capacity to grow at +50 mV was further confirmed in four additional taxa (Pecoramyces ruminatium, Anaeromyces mucronatus, Aklioshbmyces papillarum, and Piromyces communis), while two (Aestipascuomyces dupliciliberans and Capellomyces foraminis) failed to grow under these conditions. Our results establish the ability of AGF to grow at redox potential values higher than those encountered in their natural habitats. Such capability could contribute to efficient AGF dispersal and horizontal transmission between hosts, and could have important implications for industrial applications of AGF.

Important questions remain about the sources of transmission of pneumococcus to older adults in the community. This is critical for understanding the potential effects of using pneumococcal conjugate vaccines (PCVs) in children and older adults. For non-institutionalized individuals, we hypothesized that the most likely source of adult-to-adult transmission is within the household. We designed a longitudinal study to sample adults ≥60 years of age living in the same household (New Haven, CT, USA), without younger residents in the household. Saliva samples and social and health questionnaires were obtained every 2 weeks for a period of 10 weeks. DNA extracted from culture-enriched saliva was tested using qPCR for pneumococcus genes piaB, lytA, and serotype. Across two study seasons (November 2020-August 2021, November 2021-September 2022), 121 individuals from 61 households completed all six visits; 62 individuals were enrolled in both seasons. Overall, 52/1088 (4.8%) samples tested positive for pneumococcus, with 27/121 (22.3%) individuals colonized at least once. Several individuals were colonized at multiple time points; two individuals were colonized at 5/6 time points and two at all six. In 5 instances, both household members were carriers in the same season, though not necessarily at the same time. Pneumococcal carriage was substantially higher among individuals who had contact with children (10.0% vs. 1.6%). Contact with young children was the most important factor that influenced pneumococcal acquisition rates. While there were several instances where both adult household members were colonized at the same time or at sequential visits, these individuals typically had contact with children. As such, PCV immunization can directly protect older adults who have contact with children.

Enterococcus faecalis is ranked among the top five bacterial pathogens responsible for catheter-associated urinary tract infections, wound infections, secondary root canal infections, and infective endocarditis. Previously, we showed that inactivation of either the manganese- and iron-binding (EfaA) or zinc-binding (AdcA and AdcAII) lipoproteins significantly reduced E. faecalis virulence. Here, we explored whether immunization using a multi-valent approach induces protective immunity against systemic enterococcal infections. We found that multi-antigen antisera raised against EfaA, AdcA, and AdcAII displayed similar capacities to initiate neutrophil-mediated opsonization, like their single-antigen counterparts. Further, these antigen-specific antibodies worked synergistically with calprotectin, a divalent host metal chelator, to inhibit the growth of E. faecalis in laboratory media as well as in human sera. Using the Galleria mellonella invertebrate model and mouse peritonitis model, we showed that passive immunization with multi-antigen antisera conferred robust protection against E. faecalis infection, while the protective effects of single antigen antisera were negligible in G. mellonella, and negligible-to-moderate in the mouse model. Lastly, active immunization with the 3-antigen (trivalent) cocktail significantly protected mice against either lethal or non-lethal E. faecalis infections, with this protection appearing to be far-reaching based on immunization results obtained with contemporary strains of E. faecalis and closely related Enterococcus faecium.