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Neuralized Mouse Embryonic Stem Cells Develop Neural Rosette-Like Structures in Response to Retinoic Acid and Produce Teratomas in the Brains of Syngeneic Mice 神经化小鼠胚胎干细胞响应维甲酸形成神经玫瑰花样结构并在同基因小鼠大脑中产生畸胎瘤
Pub Date : 2013-12-20 DOI: 10.3968/J.ANS.1715787020130604.2838
Cheryl L Dunham, M. Kirk
Several induction protocols can direct differentiation of mouse embryonic stem cells (ESCs) to become neural cells. The B5 and B6 mouse ESC lines display different growth patterns in vitro, and when grown as adherent cultures, the B6 ESCs proliferated at a significantly lower rate than B5 ESCs. Remarkably, after a neural induction protocol that includes removal of LIF and addition of retinoic acid (RA), mature B6 embryoid bodies (EBs) displayed a unique neural rosette-like morphology. On Day 8 of neural induction, B6 EBs revealed mature neuronal markers localized primarily to cells in the center of the EBs and glial markers expressed both in centrally and peripherally located cells. In contrast to B5 cells, when neuralized Day 8 B6 EB cells were dissociated and transplanted into the left striatum of syngeneic C57BL/6 mouse brains, teratomas formed. In addition, teratomas established from undifferentiated B6 cells grew more rapidly and achieved larger volumes when compared to those produced by Day 8, neuralized B6 EBs. The slow growth rate of B6 cells in vitro may have contributed to incomplete neuralization, formation of neural rosette-like structures, and a propensity to form teratomas.
几种诱导方法可以引导小鼠胚胎干细胞(ESCs)向神经细胞分化。B5和B6小鼠ESC系在体外培养中表现出不同的生长模式,在贴壁培养时,B6 ESC的增殖率明显低于B5 ESC。值得注意的是,在去除LIF和添加维甲酸(RA)的神经诱导方案后,成熟的B6胚状体(EBs)显示出独特的神经玫瑰花状形态。在神经诱导的第8天,B6 EBs显示主要定位于EBs中心细胞的成熟神经元标记物和中枢和周围细胞中表达的胶质标记物。与B5细胞相反,当神经化第8天B6 EB细胞分离并移植到同系C57BL/6小鼠大脑左侧纹状体时,形成畸胎瘤。此外,与第8天神经化的B6细胞相比,未分化的B6细胞形成的畸胎瘤生长更快,体积更大。体外培养的B6细胞生长速度缓慢可能导致神经化不完全,形成神经玫瑰花样结构,并容易形成畸胎瘤。
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引用次数: 1
Biological Significance of Spicy Essential Oils 辛辣精油的生物学意义
Pub Date : 2013-12-20 DOI: 10.3968/J.ANS.1715787020130604.1638
S. Maurya, A. K. Kushwaha, Gurdip Singh
Spices are used as food additives since ancient times, as flavouring agents but also as natural food preservatives. Spice essential oils are complex mixtures of volatile substances, ordinarily terpenes, sesquiterpenes and oxygenated derivatives. They have been largely employed for their properties already observed in nature i.e., for their antibacterial, antifungal and insecticidal properties. At present, approximately 3000 essential oils are known, 300 of which are commercially important especially for pharmaceutical, agronomic, food, sainitary, cosmetic and perfume industries. It is important to develop a better understanding of their mode of action for new applications in human health, agriculture and environment. Some of them constitute effective alternatives or complements to synthetic compounds of chemical industry.
香料自古以来就被用作食品添加剂,用作调味剂,也用作天然食品防腐剂。香料精油是挥发性物质的复杂混合物,通常是萜烯、倍半萜烯和含氧衍生物。它们在很大程度上是因为它们在自然界中已经观察到的特性而被使用的,即它们的抗菌、抗真菌和杀虫特性。目前,已知的精油大约有3000种,其中300种具有重要的商业价值,特别是在制药、农艺、食品、卫生、化妆品和香水行业。重要的是要更好地了解它们的作用方式,以便在人类健康、农业和环境方面有新的应用。其中一些是化学工业合成化合物的有效替代品或补充物。
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引用次数: 8
Spectrophotometric Determination of Methyl Paraben in Pure and Pharmaceutical Oral Solution 分光光度法测定纯和药用口服液中对羟基苯甲酸甲酯的含量
Pub Date : 2013-12-20 DOI: 10.3968/J.ANS.1715787020120604.2601
S. Dhahir, H. Hussein
A simple, cheap and sensitive spectrophotometric method for the determination of methyl paraben in pure and dosage form has been described. The Method is based on the diazotization of the drug by sodium nitrite in acidic medium at 5Co followed by coupling with ortho-aminobenzoic acid to form orange color the product was stabilized and measured at 442 nm Beer’s law is obeyed in the concentration range of 1-9 μg∙ml-1 with molar absorptivity of 1.6x106 L∙mole-1∙cm-1., Sandell’s sensitivity were 0.0095 μg∙cm-1, The detection limit were 0.0065 μg∙ml-1, and The limit of Quantitation were 0.02 μg∙ml-1. All variables including the reagent concentration, reaction time, color stability period, and mole ratio were studied in order to optimize the reaction conditions. No interferences were observed Results of analysis were validated statistically and by recovery studies. These methods are successfully employed for the determination of methyl paraben in some oral solution. The developed method is easy to use and accurate for routine studies relative to HPLC and other techniques.
本文介绍了一种简单、廉价、灵敏的分光光度法测定对羟基苯甲酸甲酯的纯度和剂型。该方法采用亚硝酸钠在酸性介质中5Co重氮化,然后与邻氨基苯甲酸偶联形成橙色,稳定后在442 nm下测定,浓度范围为1 ~ 9 μg∙ml-1,摩尔吸光度为1.6 × 106 L∙mol -1∙cm-1。Sandell的灵敏度为0.0095 μg∙cm-1,检测限为0.0065 μg∙ml-1,定量限为0.02 μg∙ml-1。考察了试剂浓度、反应时间、色稳期、摩尔比等因素对反应条件的影响。未观察到干扰,分析结果经统计学和回收率研究验证。本方法成功地测定了某些口服液中对羟基苯甲酸甲酯的含量。相对于高效液相色谱法和其他技术,该方法操作简便,准确度高。
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引用次数: 10
Nuclear Energy From the Fission Process as an Alternative Source of Energy 核能从裂变过程中作为一种替代能源
Pub Date : 2013-12-20 DOI: 10.3968/J.ANS.1715787020130604.2861
W. Lumbi, I. I. Ewa, T. Y. Woma
One of the major problems hindering the development of most developing countries, including Nigeria, is energy. Over the years, Nigeria’s population has continued to increase, nearly doubling in past thirty years. The energy consumption has also been on the increase, even increasing more rapidly than the population. If the trend in electrical energy consumption continues to increase, conventional energy resources used to generate electricity will be depleted; hence, there will be the need for other sources of energy. In this paper we discussed non-conventional source of energy as alternative solution to the energy crisis in Nigeria with emphasis on fission technique for the generation of nuclear energy. The study reveals that nuclear energy looks attractive and merits good consideration and support because of low operating cost particularly at this time of high price of fossil fuel.
阻碍包括尼日利亚在内的大多数发展中国家发展的主要问题之一是能源。多年来,尼日利亚的人口持续增长,在过去的30年里几乎翻了一番。能源消耗也在增加,甚至比人口增长的速度还要快。如果电能消耗的趋势继续增加,用于发电的常规能源将会枯竭;因此,将需要其他能源。在本文中,我们讨论了非常规能源作为替代解决方案的能源危机在尼日利亚,重点是裂变技术的核能发电。研究表明,特别是在目前化石燃料价格高企的情况下,核能具有较低的运行成本,具有很大的吸引力,值得考虑和支持。
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引用次数: 0
Antiasthmatic Effect of Standardized Extract of Ayurvedic Compounds via Nasal Spray Actuation (in Aerosol Form) in Rodents and Its Comparative Clinical Study 阿育吠陀化合物标准提取物经鼻喷雾驱动(气雾剂形式)对啮齿动物的平喘作用及其比较临床研究
Pub Date : 2013-09-26 DOI: 10.3968/3729
K. Divya, J. Tripathi, J. Samaria, S. Tiwari
Abstract Asthma is one of the most prevalent chronic inflammatory lung disease among children and adults. Morbidity and mortality rate of asthma is raising at the rate of 10% every year inspite of availability of contemperory modern medicines. Studies show that presently majority of world population are using complementory and altenative medicines for the management of bronchial asthma. Ayurveda (Indian system of medicine) is the most popular and widely used alternative medicine. Extensive and proficient researches in the field of Ayurveda validate its efficay in the mangement of chronic diseases including asthma. The present study was designed to search a safe and effective herbal medicine for the treatment of acute attack of Bronchial Asthma and secondary objective to expand the sphere of Ayurvedic medicine by exploring the new possibilities of drug administration techniques.Hydroethanolic extract of both the polyherbal compounds were given through nebulization and their efficacy was assessed. Results showed that all groups treated with Ayurvedic compounds had significant improvent in PFT (pulmonary function test). Cough, Expectoration and ESR is markedly reduced in group treaed with Bharangyadi Ayurvedic Nebulizer whereas Wheezing, frequency of attack and severity of attack on exposure to allergens was significantly (p < 0.001) reduced in group treated with Shirishadi Ayurvedic Nebulizer.
哮喘是儿童和成人中最常见的慢性炎症性肺部疾病之一。尽管有当代现代药物,哮喘的发病率和死亡率仍以每年10%的速度上升。研究表明,目前世界上大多数人都在使用补充和替代药物来治疗支气管哮喘。阿育吠陀(印度医学体系)是最受欢迎和广泛使用的替代医学。在阿育吠陀领域广泛而精通的研究证实了它在治疗包括哮喘在内的慢性疾病方面的有效性。本研究旨在寻找一种安全有效的治疗支气管哮喘急性发作的草药,第二目的是通过探索药物给药技术的新可能性来扩大阿育吠陀医学的领域。通过雾化给药,对两种复方水乙醇提取物进行疗效评价。结果显示,阿育吠陀治疗组肺功能均有明显改善。使用巴朗雅迪阿育吠陀雾化器治疗组咳嗽、咳痰和ESR明显减少,而使用Shirishadi阿育吠陀雾化器治疗组的哮喘、发作频率和暴露于过敏原的发作严重程度显著(p < 0.001)降低。
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引用次数: 0
General Relativistic Equation of Motion for a Photon Moving Round a Time Varying Spherical Distribution of Mass 光子绕时变球形质量分布运动的广义相对论方程
Pub Date : 2013-09-26 DOI: 10.3968/3731
W. Lumbi, I. I. Ewa
Abstract In this article, Schwarzschild metric is extended to obtain a generalized metric for the gravitational field exterior to time varying spherical distributions of mass. Einstein’s equation for photon moving round a time varying spherical distribution of mass is derived. The second-order differential equation obtained is a modification of the equation of motion in Schwarzschild field. It introduces a unique dependence of the motion of the photon in this field on Newton’s scalar potential exterior to time varying spherical bodies.
摘要本文将史瓦西度规进行扩展,得到了质量随时间变化的球形分布之外的引力场的广义度规。推导了光子绕时变球面质量分布运动的爱因斯坦方程。所得到的二阶微分方程是对史瓦西场运动方程的修正。它引入了光子在该场中的运动对时变球体外的牛顿标量势的独特依赖。
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引用次数: 2
Bath Adsorption Study of Methylene Blue Dye Onto Sunflower Seeds Husks Activated Carbon 亚甲基蓝染料在葵花籽壳活性炭上的吸附研究
Pub Date : 2013-09-26 DOI: 10.3968/J.ANS.1715787020130603.2596
J. Salman, F. D. Almutairi
Mesoporous activated carbon prepared from sunflower seeds husks (SSH) using physiochemical activation (potassium hydroxide treatment and carbon dioxide gasification). The optimum conditions for preparing activated carbon from (SSH) were found to be activation temperature of 500 oC, activation time of 1.0 h and chemical impregnation ratio of 1:1. The adsorptions of methylene blue (MB) onto sunflower seed husks activated carbon (SSHAC) were studied with respect to initial MB concentration. The experimental data were analyzed by the Langmuir isotherm, the Freundlich isotherm and the Temkin isotherm. Equilibrium data fitted well with the Langmuir model with maximum adsorption capacity of 410 mg/g at 30 oC for MB concentration range of 50-300 mg/L. Desorption-adsorption studies for the spent SSHAC (saturated with MB) using ethanol as solvent showed that regeneration efficiency around 85%. The results indicated that the SSHAC is very effective for the adsorption of MB from aqueous solutions and can be regenerate to use for more than two adsorption cycles.
以葵花籽壳(SSH)为原料,经氢氧化钾处理和二氧化碳气化制备介孔活性炭。以(SSH)为原料制备活性炭的最佳工艺条件为:活化温度500℃,活化时间1.0 h,化学浸渍比1:1。研究了亚甲基蓝(MB)在葵花籽壳活性炭(SSHAC)上的吸附性能。用Langmuir等温线、Freundlich等温线和Temkin等温线对实验数据进行了分析。平衡数据符合Langmuir模型,在30℃条件下,MB浓度范围为50 ~ 300 mg/L,最大吸附量为410 mg/g。以乙醇为溶剂对饱和MB的废SSHAC进行解吸吸附研究,再生效率在85%左右。结果表明,SSHAC对MB的吸附效果良好,可再生使用两次以上。
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引用次数: 6
Health Benefits, Chemistry and Mechanism of Carica Papaya a Crowning Glory 木瓜的健康益处、化学成分及机理
Pub Date : 2013-09-26 DOI: 10.3968/3732
Ateeq Rahman
Abstract Mini review article highlights about the health benefits, chemistry and mechanism of papaya. Fruit and vegetables cannot be equated to that promised by nutritional pills and supplements. Nutrition experts advocate generous intake of fruits for optimum health as these food items are loaded with all the benefits. Especially papaya fruits is goldmine of vitamins C, E, K, minerals and fiber and ideal to consume at least 4-5 servings in a day. Since they are in natural form, account for largest part of water and 100% bad cholesterol free, it’s much easier for the body to process and absorb the vitamins and minerals from the fresh fruit. The chemical action of acyltransferase is studied which is extracted from ripened papaya fruit and then benzyl alcohol is acylated to give benzylacetate.
摘要:本文主要介绍木瓜的健康益处、化学成分和作用机理。水果和蔬菜不能等同于营养药片和补充剂所承诺的那样。营养专家提倡多吃水果以达到最佳健康状态,因为这些食物富含所有的益处。尤其是番木瓜是维生素C、E、K、矿物质和纤维的金矿,一天至少要吃4-5份。因为它们是天然的,占水分的大部分,100%不含有害胆固醇,所以身体更容易加工和吸收新鲜水果中的维生素和矿物质。研究了成熟番木瓜果实中提取的酰基转移酶的化学作用,并对苯甲醇进行酰基化,得到乙酸苄酯。
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引用次数: 12
Spectral Analysis of Catalytic Oxidation and Degradation of Bromophenol Blue at Low pH with Potassium Dichromate 低pH下重铬酸钾催化氧化降解溴酚蓝的光谱分析
Pub Date : 2013-09-26 DOI: 10.3968/3734
R. Azmat, Z. Khalid, M. Haroon, Khalida Perveen Mehar
Abstract This research investigated the efficacy of potassium dichromate (K2Cr2O7), ultraviolet (UV) solar irradiation and UV in combination with two diprotic acid i-e sulphuric and oxalic acid (UV chromate/carbonate), for decolorizing bromophenol blue (BPB). The results suggested that alone UV and solar exposure was the poor decolorizer of the dye waste water. Up to 10% color removal with alone UV and solar treatment were achieved which indicated that, only solar and UV were not effective for dye removal even though UV was slightly more effective as compared to solar one. The decline reaction rate was observed as a result of increased concentration of dye. Increase in concentration of dichromate showed two peaks at 592 and 515 which showed that bromophenol degraded into two smaller components through complex formation. Addition of sodium carbonate as a catalyst in dye chromate-oxalate system found to be effective in degradation of BPB with reduced time period. The reaction was rapid and almost maximum of the dye converted into CO2. Research outcome suggests that chromate-oxalate system is very effective techniques for reducing color of dye waste water for safety of aquatic environment. Reaction pathway of oxidation of BPB is discussed in the relevant section of the paper.
摘要本研究考察了重铬酸钾(K2Cr2O7)、紫外(UV)太阳照射和紫外(UV)与两种二质子酸i-e硫酸和草酸(UV铬酸盐/碳酸盐)联合对溴酚蓝(BPB)的脱色效果。结果表明,单独紫外线照射和日光照射对染料废水脱色效果较差。单独使用紫外线和太阳能处理可达到高达10%的脱色效果,这表明,仅使用太阳能和紫外线对脱色无效,尽管紫外线比太阳能稍微有效一些。随着染料浓度的增加,反应速率下降。重铬酸盐浓度的增加在592和515处出现两个峰,表明溴酚通过络合物的形成降解为两个较小的组分。在染料铬酸盐-草酸盐体系中加入碳酸钠作为催化剂,可以有效地降低BPB的降解时间。反应迅速,几乎大部分染料转化为二氧化碳。研究结果表明,铬酸盐-草酸盐体系是一种有效的染料废水脱色技术,可保证水环境的安全。对BPB氧化的反应途径进行了讨论。
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引用次数: 9
Investigation of Ultraviolet Radiation (UVR) in Benue State University, Makurdi, Benue State 贝努埃州立大学马库尔迪分校紫外线辐射(UVR)调查
Pub Date : 2013-06-26 DOI: 10.3968/J.ANS.1715787020120602.2017
N. Akaagerger, F. O. Ujah, E. H. Aligba
The study investigated the level of ultraviolet (UV) radiation in Benue State University, Makurdi. Since the greatest source of UV radiation reaching the earth surface is from the sun, we have investigated in detail the solar UV intensity in Benue State University. This was done through measurement of UV indices with the use of a broad band UV checker which infers the intensity of UV radiation. Typical values of ultraviolet indices between 7 and 13 were observed during clear sky hours in the solar noon hours of the day, meaning the UV radiation intensity was high. However,it was observed to be low/moderate between UVI of 1 and 6 when overcast occurred in the sky. But the intensities of UV radiation were observed to be constantly low/moderate from 6:00am-10:00am and 4:00pm-5:00pm times of the day.
该研究调查了马库尔迪贝努埃州立大学的紫外线辐射水平。由于到达地球表面的紫外线辐射的最大来源是太阳,我们在贝努埃州立大学详细研究了太阳紫外线强度。这是通过使用宽频带紫外线检测器测量紫外线指数来推断紫外线辐射的强度。在正午晴空时段,紫外线指数的典型值在7至13之间,表明紫外线辐射强度较高。然而,当天空出现阴天时,观测到它的紫外线指数介于1至6之间的低/中。但在上午6时至10时和下午4时至5时,紫外线的强度持续低/中。
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引用次数: 0
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Advances in Natural Science
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