Journal of clinical virology plus最新文献_第4页

Diagnosis of SARS-CoV-2: Experience with rapid immunochromatography tests and RT‒qPCR 诊断 SARS-CoV-2：快速免疫层析测试和 RT-qPCR 的经验

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-03-31 DOI: 10.1016/j.jcvp.2025.100207

David Alejandro Cabrera Gaytán , Porfirio Felipe Hernández Bautista , Clara Esperanza Santacruz Tinoco , Julio Elias Alvarado Yaah , Yu-Mei Anguiano Hernández , Bernardo Martínez Miguel , Alfonso Vallejos Parás , Lumumba Arriaga Nieto , Nancy Sandoval Gutiérrez , Concepción Grajales Muñiz , Alejandro Moctezuma Paz , Leticia Jaimes Betancourt

Background

Mexico has experienced six waves were experiences between 2019 and 2023 of coronavirus disease 2019 (COVID-19). In the first wave, diagnosis relied on RT‒qPCR; rapid tests for SARS‒CoV‒2 were introduced from the second wave onward. However, the patterns of results with this test in Mexico have not been documented in population-based studies.

Objective

To describe the temporal patterns of the results from RT-qPCR and rapid immunochromatography tests for SARS-CoV-2 detection from the second to the sixth epidemic wave in Mexico.

Materials and methods

This was a multicenter study at the national level. The operational definition of suspected cases of viral respiratory illness, confirmed cases of SARS-CoV-2 in Mexico, and data for asymptomatic individuals who required rapid testing between 2020 and 2023 were employed; testing was employed for the entirety of the four years. Positivity was estimated per epidemiological week, state, and condition for both tests. Spearman's correlation coefficient, trend chi-square analysis, and odds ratios with 95 % confidence intervals were used.

Results

A total of 1749,765 cases had RT‒qPCR data recorded from 2020 to 2023, and 8356,903 cases with rapid tests were conducted for virus identification. Compared with the rest of the country, the southeastern region exhibited different patterns. The positivity rate of rapid tests from the epidemiological surveillance system was lower than that of RT‒qPCR during interepidemics periods, whereas the positivity rate of rapid tests for symptomatic individuals was higher than that of RT‒qPCR tests over two years.

Conclusion

Rapid tests for SARS-CoV-2 identification in Mexico were affordable and timely at the local level. These tests revealed differing epidemic wave patterns in the southeastern region of the country.

墨西哥在2019年至2023年期间经历了六波2019冠状病毒病（COVID-19）。在第一波中，诊断依靠RT-qPCR；从第二波开始引入了SARS-CoV-2的快速检测。然而，在以人口为基础的研究中尚未记录墨西哥使用该测试的结果模式。目的分析墨西哥第二至第六波SARS-CoV-2流行的RT-qPCR和快速免疫层析检测结果的时间分布规律。材料与方法本研究为国家级多中心研究。采用了病毒性呼吸道疾病疑似病例、墨西哥SARS-CoV-2确诊病例的操作定义，以及2020年至2023年间需要快速检测的无症状个体数据；整个四年都在进行测试。根据流行病学周、州和条件估计两项检测的阳性。采用Spearman相关系数、趋势卡方分析和95%置信区间的优势比。结果2020 - 2023年共记录RT-qPCR数据1749765例，进行病毒快速鉴定8356903例。与全国其他地区相比，东南部地区表现出不同的格局。流行病学监测系统快速检测在流行间期的阳性率低于RT-qPCR，而2年以上有症状个体快速检测的阳性率高于RT-qPCR。结论墨西哥的SARS-CoV-2快速检测在地方层面上是负担得起且及时的。这些检测揭示了该国东南部地区不同的流行波模式。

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引用次数: 0

Persistent nucleic acids from endemic human coronaviruses in adenoids: Do they enhance children's immune response to SARS-CoV-2 infection? 腺样体中地方性人类冠状病毒的持久性核酸：它们是否增强了儿童对SARS-CoV-2感染的免疫反应？

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-03-25 DOI: 10.1016/j.jcvp.2025.100208

Kira Winkler , Lucia Otten , Alina Abramian , Eva Weber , Florian Winkler , Niklas Klümper , Anna Maria Schmidt , Zahrasadat Safavieh , Anna Maria Eis-Hübinger , Stephan Herberhold

Introduction

Human Coronaviruses (HCoVs), including HCoV-OC43, HCoV-HKU1, HCoV-NL63, and HCoV-229E, are endemic viruses that circulate globally and infect humans. In contrast to SARS-CoV-2, which causes more severe symptoms, these endemic HCoVs typically induce mild cold symptoms and confer only temporary immunity [1,2]]. Understanding the presence and persistence of HCoVs in the human population, particularly in asymptomatic individuals, is crucial for understanding their potential role in immunity and viral dynamics.

Methods

This study aimed to investigate the presence of nucleic acids of endemic HCoVs (HCoV-OC43, HCoV-HKU1, HCoV-NL63, and HCoV-229E) in individuals without symptoms of acute respiratory infection. A total of 78 adenoid tissue samples were collected from children (up to 10 years old) without current symptoms of airway infection. The samples were analyzed using RT-nested PCR to detect viral RNA.

Results

Of the 78 adenoid samples, 24 (30,8 %) tested positive for at least one type of endemic HCoV. Additionally, throat swabs were collected from 56 participants immediately before surgery. Endemic HCoV RNA was rarely detected in these throat swabs, with only 3 out of 56 samples testing positive.

Discussion

Our findings suggest the frequent presence of endemic HCoV nucleic acids in the lymphatic tissue of Waldeyer's ring, especially in children. This observation supports the concept of these viruses acting as immune triggers. The persistence of viral remnants in the adenoid tissue may contribute to continuous immune surveillance, which could have implications for immunity to future infections and for understanding viral dynamics in asymptomatic individuals.

人类冠状病毒（hcov），包括HCoV-OC43、HCoV-HKU1、HCoV-NL63和HCoV-229E，是全球传播并感染人类的地方性病毒。与引起更严重症状的SARS-CoV-2相比，这些地方性hcov通常会引起轻微的感冒症状，并仅赋予暂时的免疫力[1,2]]。了解hcov在人群中的存在和持续存在，特别是在无症状个体中，对于了解其在免疫和病毒动力学中的潜在作用至关重要。方法本研究旨在调查无急性呼吸道感染症状个体中地方性hcov （HCoV-OC43、HCoV-HKU1、HCoV-NL63和HCoV-229E）的核酸存在情况。从目前没有呼吸道感染症状的儿童（10岁以下）共收集了78个腺样体组织样本。采用rt -巢式PCR检测病毒RNA。结果78例腺样体标本中，24例（30.8%）至少检出一种地方性HCoV阳性。此外，在手术前立即收集了56名参与者的咽拭子。在这些咽拭子中很少检测到地方性HCoV RNA， 56个样本中只有3个检测呈阳性。我们的研究结果表明，在瓦尔德耶氏环的淋巴组织中经常存在地方性HCoV核酸，特别是在儿童中。这一观察结果支持了这些病毒作为免疫触发器的概念。腺样体组织中病毒残留物的持续存在可能有助于持续的免疫监视，这可能对未来感染的免疫和了解无症状个体的病毒动力学具有重要意义。

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引用次数: 0

Urgent attention for health care services preparedness to address rising human metapneumovirus (HMPV) cases 紧急关注卫生保健服务准备，以应对不断上升的人偏肺病毒（HMPV）病例

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-03-24 DOI: 10.1016/j.jcvp.2025.100209

Md. Salman Sohel , Safayet Jamil , Md. Naimur Rahman

引用次数: 0

The frequency of post-COVID-19 condition and its clinical relevance after COVID-19 disease of employees in facilities of preschool childcare 学龄前托幼机构工作人员新冠肺炎后发病频次及其临床相关性

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2024.100201

Michelle Nina Braun , Anna Wolfschmidt , Andrea Kaifie , Ruediger Stephan Goertz

Objective

Evaluation of the rate and symptoms of Post-COVID-19 condition in employees in childcare.

Methods

A structured questionnaire collected data from two distinct collectives: Collective 1 (C1) consisted of a prospective cohort of 44 employees after COVID-19 disease, whereas Collective 2 (C2) included 222 employees who were retrospectively interviewed. The questionnaire mainly focused on symptoms and wellbeing during or after COVID-19.

Results

The main symptoms of Post-COVID-19 condition were impairment of focus, fatigue/exhaustion, reduced stamina, headache and dyspnoea. 30 % of the employees in C1 and 7.6 % of C2 suffered from a clinically relevant Post-COVID-19 condition with impairment of the general wellbeing. According to the WHO definition, a Post-COVID-19 condition was found in 56.6 % (C1) and 25.5 % (C2).

Conclusions

A Post-COVID-19 condition was a common finding in both our collectives but did not automatically cause an impairment of general wellbeing.

目的了解托儿人员新型冠状病毒感染后症状及发生率。方法采用结构化问卷收集来自两个不同群体的数据：集体1 （C1）包括44名COVID-19疾病后的员工，而集体2 （C2）包括222名回顾性访谈的员工。问卷主要关注COVID-19期间或之后的症状和健康状况。结果新冠肺炎后患者的主要症状为注意力障碍、疲劳/乏力、耐力下降、头痛和呼吸困难。C1区30%的员工和C2区7.6%的员工患有临床相关的后冠状病毒病，总体健康受损。根据世卫组织的定义，56.6% （C1型）和25.5% （C2型）的患者存在后冠状病毒感染。结论新冠肺炎后症状在我们两个集体中都很常见，但不会自动导致总体幸福感受损。

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引用次数: 0

Evaluation of a flow cytometry-based surrogate assay (FlowSA) for the detection of SARS-CoV-2 in clinical samples 基于流式细胞术的替代检测法（FlowSA）在临床样品中检测SARS-CoV-2的评价

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2025.100204

Vinit Upasani , Marjolein Knoester , Daniele Pantano , Lili Gard , Jolanda M. Smit , Bernardina T.F. van der Gun , Adriana Tami , Izabela A. Rodenhuis-Zybert

Introduction

The current diagnostic methods for SARS-CoV-2 rely on quantitative RT-PCR. However, the presence of viral RNA in samples does not necessarily reflect the presence of an infectious virus. Therefore, the reliable detection of infectious SARS-CoV-2 in clinical samples is necessary to limit viral transmission.

Methods

We developed a flow cytometry-based surrogate assay (FlowSA), wherein the presence of infectious SARS-CoV-2 was detected using virus nucleocapsid-specific antibodies.

Results

We showed that FlowSA allows the detection of a wide range of viral titers of multiple SARS-CoV-2 variants. Furthermore, the assay was successfully used to detect infectious SARS-CoV-2 in nasopharyngeal swabs from SARS-CoV-2 positive individuals, including those with high Ct values. Notably, FlowSA identified the presence of infectious SARS-CoV-2 in biological specimens that scored negative for cytopathic effect (CPE) in cell culture and would otherwise be considered negative.

Conclusion

We propose that FlowSA can be adopted as an alternative to conventional CPE methods for viral diagnostics.

目前SARS-CoV-2的诊断方法主要依赖于定量RT-PCR。然而，样本中病毒RNA的存在并不一定反映感染性病毒的存在。因此，在临床样本中可靠地检测传染性SARS-CoV-2是限制病毒传播的必要条件。方法我们建立了一种基于流式细胞术的替代检测方法（FlowSA），其中使用病毒核衣壳特异性抗体检测传染性SARS-CoV-2的存在。结果FlowSA可以检测多种SARS-CoV-2变体的大范围病毒滴度。此外，该方法还成功地用于检测SARS-CoV-2阳性个体（包括Ct值高的个体）鼻咽拭子中的传染性SARS-CoV-2。值得注意的是，FlowSA在细胞培养中细胞病变效应（CPE）得分为阴性的生物标本中发现了传染性SARS-CoV-2的存在，否则将被视为阴性。结论FlowSA可作为传统CPE方法的替代方法用于病毒诊断。

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引用次数: 0

Effect of daily alcohol consumption and age over 40 years on COVID-19 vaccination antibody titers in the Delta era among hospital workers in northern Okinawa, Japan: A retrospective cohort study 日本冲绳北部三角洲时期医院工作人员每日饮酒量和40岁以上年龄对COVID-19疫苗抗体滴度的影响：一项回顾性队列研究

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2025.100205

Takuji Kishimoto , Daisuke Tasato , Yoshitaka Nagasawa , Akihiro Yamashiro , Hayashi Shokita

Background

Coronavirus disease 2019 (COVID-19) vaccination has demonstrated efficacy in preventing infection, mitigating disease severity, and lowering the incidence of Long COVID. To enhance vaccine effectiveness, it is not only important to develop more effective vaccines but also to clarify factors, including lifestyle, that affect the immune response. The aim of this study was to investigate the impact of lifestyle factors on COVID-19 vaccination antibody titers.

Methods

Antibody titers of 354 hospital workers who received two COVID-19 vaccination doses were measured five times for more than six months. Information on medical history, demographic characteristics, and lifestyle-related items was obtained from hospital health checkups. The outcome variable (Lower-25 %) was defined as the antibody titer value below the 25th percentile of the fifth measurement. The Cox proportional hazard survival model was used to evaluate the hazard ratio for incidence of Lower-25 % according to lifestyle-related items.

Results

The crude incidence rates per 1,000 person-days for Lower-25 % among women and men were 1.35 and 1.66, respectively. The hazard ratios for Lower-25 % of those in their 40 s, 50 s, and 60 s compared with those in their 20 s were 5.82 (95 % confidence interval [CI], 2.05–16.51), 7.12 (95 % CI, 2.46–20.63), and 9.96 (95 % CI, 3.07–32.34), respectively. The hazard ratios for “daily” versus “never” drinking habits were 2.26 (95 % CI 1.17–4.34).

Conclusions

The results of this study indicate that shortening vaccination intervals for individuals over 40 years and discontinuing daily alcohol consumption are associated with the preservation of acquired antibody titers for optimizing vaccine efficacy.

背景2019冠状病毒病（COVID-19）疫苗接种已被证明在预防感染、减轻疾病严重程度和降低长COVID发病率方面具有疗效。为了提高疫苗的有效性，不仅要开发更有效的疫苗，而且要弄清影响免疫反应的因素，包括生活方式。本研究的目的是探讨生活方式因素对COVID-19疫苗抗体滴度的影响。方法对354名接受2剂新冠肺炎疫苗接种的医护人员进行5次抗体滴度检测，持续6个月以上。从医院健康检查中获得了病史、人口统计学特征和生活方式相关项目的信息。结果变量（低- 25%）定义为抗体滴度值低于第五次测定的第25百分位数。根据生活方式相关项目，采用Cox比例风险生存模型评估低- 25%发生率的风险比。结果低25%人群中，女性和男性的粗发病率分别为1.35和1.66。40多岁、50多岁和60多岁的低25%患者与20多岁患者的风险比分别为5.82（95%可信区间[CI]， 2.05 ~ 16.51）、7.12 （95% CI, 2.46 ~ 20.63）和9.96 （95% CI, 3.07 ~ 32.34）。“每日”饮酒习惯与“从不”饮酒习惯的风险比为2.26 （95% CI 1.17-4.34）。结论缩短40岁以上人群的疫苗接种间隔和停止每日饮酒与保存获得性抗体滴度有关，从而优化疫苗效力。

{"title":"Effect of daily alcohol consumption and age over 40 years on COVID-19 vaccination antibody titers in the Delta era among hospital workers in northern Okinawa, Japan: A retrospective cohort study","authors":"Takuji Kishimoto , Daisuke Tasato , Yoshitaka Nagasawa , Akihiro Yamashiro , Hayashi Shokita","doi":"10.1016/j.jcvp.2025.100205","DOIUrl":"10.1016/j.jcvp.2025.100205","url":null,"abstract":"<div><h3>Background</h3><div>Coronavirus disease 2019 (COVID-19) vaccination has demonstrated efficacy in preventing infection, mitigating disease severity, and lowering the incidence of Long COVID. To enhance vaccine effectiveness, it is not only important to develop more effective vaccines but also to clarify factors, including lifestyle, that affect the immune response. The aim of this study was to investigate the impact of lifestyle factors on COVID-19 vaccination antibody titers.</div></div><div><h3>Methods</h3><div>Antibody titers of 354 hospital workers who received two COVID-19 vaccination doses were measured five times for more than six months. Information on medical history, demographic characteristics, and lifestyle-related items was obtained from hospital health checkups. The outcome variable (Lower-25 %) was defined as the antibody titer value below the 25th percentile of the fifth measurement. The Cox proportional hazard survival model was used to evaluate the hazard ratio for incidence of Lower-25 % according to lifestyle-related items.</div></div><div><h3>Results</h3><div>The crude incidence rates per 1,000 person-days for Lower-25 % among women and men were 1.35 and 1.66, respectively. The hazard ratios for Lower-25 % of those in their 40 s, 50 s, and 60 s compared with those in their 20 s were 5.82 (95 % confidence interval [CI], 2.05–16.51), 7.12 (95 % CI, 2.46–20.63), and 9.96 (95 % CI, 3.07–32.34), respectively. The hazard ratios for “daily” versus “never” drinking habits were 2.26 (95 % CI 1.17–4.34).</div></div><div><h3>Conclusions</h3><div>The results of this study indicate that shortening vaccination intervals for individuals over 40 years and discontinuing daily alcohol consumption are associated with the preservation of acquired antibody titers for optimizing vaccine efficacy.</div></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"5 1","pages":"Article 100205"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143135904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative evaluation of APRI and MELD scores in the prediction of complications of chronic hepatitis in patients infected with hepatitis B or C viruses in Lubumbashi, democratic republic of Congo 在刚果民主共和国卢本巴希，APRI和MELD评分预测乙型或丙型肝炎病毒感染患者慢性肝炎并发症的比较评价

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2024.100200

Arsène Kabamba-Tshikongo , Adolphe Baleebenga , Bernard Kalunga-Tompa , Claude Mwamba-Mulumba , Henry Manya-Mboni , Cedrick Mutombo-Shakalenga , Pierrot Mwamba-Tshilumba , Sébastien Bontems , Géraldine Dessilly , Benoît Kabamba-Mukadi , Albert Longanga-Otshudi

The indication for treatment of viral hepatitis B and C depends on the degree of deterioration of liver function and secondarily viral load. APRI and MELD scores are WHO-validated tests for the assessment of liver fibrosis. They are inexpensive and non-invasive. Many patients suffer from viral hepatitis B or C in the Democratic Republic of Congo. These scores are an asset in the medical management of these patients. The objective of this study was to compare APRI and MELD scores in the prediction of fibrosis in patients with chronic viral hepatitis B in Lubumbashi. It included infected patients followed in the gastroenterology departments of the hospitals of Lubumbashi and the Center of Excellence and Expertise for Viral Hepatitis and other Pathologies (CEEHP). Biological parameters of patient samples were evaluated to calculate APRI and MELD scores. Analytical performance of APRI and MELD scores was evaluated by using liver biopsy as gold standard.

The APRI and MELD scores had a sensitivity of 100 % and a specificity of 93.5 and 88.6 %, respectively. In the fibrosis group of patients infected with HBV, the mean APRI score was of 1.89 whereas the mean MELD score was of 12.4 Incorporating these scores in routine clinical practice could reduce the morbidity rate, the costs related to the practice of liver biopsy and thus improve the routine management of viral hepatitis in Lubumbashi.

病毒性乙型肝炎和丙型肝炎治疗的适应症取决于肝功能恶化的程度和继发病毒载量。APRI和MELD评分是世卫组织认可的评估肝纤维化的检测方法。它们价格低廉且无侵入性。在刚果民主共和国，许多患者患有病毒性乙型或丙型肝炎。这些分数在这些病人的医疗管理中是一个资产。本研究的目的是比较APRI和MELD评分在卢本巴希地区预测慢性乙型病毒性肝炎患者纤维化的作用。它包括在卢本巴希医院的胃肠科和病毒性肝炎及其他病症卓越和专业知识中心（CEEHP）随访的感染患者。评估患者标本的生物学参数，计算APRI和MELD评分。以肝活检为金标准评价APRI和MELD评分的分析性能。APRI和MELD评分的敏感性为100%，特异性为93.5%和88.6%。在HBV感染的纤维化组中，APRI平均评分为1.89，MELD平均评分为12.4，将这些评分纳入常规临床实践可以降低发病率，降低肝活检相关费用，从而改善卢本巴希地区病毒性肝炎的常规管理。

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引用次数: 0

Seroprevalence of hepatitis B and C virus among a group of hemophilia patients in Kurdistan Region, Iraq 伊拉克库尔德斯坦地区一组血友病患者中乙型和丙型肝炎病毒的血清阳性率

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2025.100203

Salih A. Hama

Background

The risk of blood-borne infections, particularly hepatitis B virus (HBV) and hepatitis C virus (HCV), continues to persist in developing countries among patients who receive blood products, such as hemophiliacs, but there is a lack of up-to-date data in such countries. This study aims to determine the prevalence of HBV and HCV infections among a group of hemophilia patients in Kurdistan Region, Iraq.

Methods

This prospective cross-sectional study, conducted from June to September 2022, screened 117 hemophilic patients in the region for Hepatitis B surface antigen (HBsAg) and anti-HCV antibodies using serologic assays.

Results

One hundred fifteen participants were male (98.3 %) with a mean age of 20.7 years (8–47). HBV seropositivity was found in 9 (7.7 %) patients, while HCV seropositivity was noted in 15 (12.8 %). No significant correlation was observed between HBV/HCV infectivity status and age, residency, or education status (p < 0.05). The prevalence of only HBV was significantly higher among patients with more frequent clotting factor intake (p = 0.016), which was associated with lower odds of HBV positivity (OR = 0.034, 95 % CI: 0.002–0.492). Moreover, HCV seropositive patients were more commonly associated with surgical history compared to negative cases (20 %vs. 3.9 %; p = 0.044), which was associated with significantly increased odds of HCV positivity (OR = 6.125, 95 % CI: 1.221–30.719).

Conclusion

This study reveals an intermediate prevalence of HBV (7.7 %) and a high prevalence of HCV (12.8 %) infections among hemophilia patients in the Kurdistan Region, Iraq.

在发展中国家，血友病患者等接受血液制品的患者中，血液传播感染的风险，特别是乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）的风险持续存在，但这些国家缺乏最新数据。本研究旨在确定伊拉克库尔德斯坦地区一组血友病患者中HBV和HCV感染的流行情况。该前瞻性横断面研究于2022年6月至9月进行，通过血清学检测筛查该地区117名血友病患者的乙型肝炎表面抗原（HBsAg）和抗hcv抗体。结果男性115例（98.3%），平均年龄20.7岁（8 ~ 47岁）。HBV血清阳性9例（7.7%），HCV血清阳性15例（12.8%）。HBV/HCV感染状况与年龄、居住地或教育状况之间无显著相关性(p <；0.05)。在凝血因子摄入频繁的患者中，仅HBV的患病率明显较高（p = 0.016），这与HBV阳性的几率较低相关（OR = 0.034, 95% CI: 0.002-0.492）。此外，与阴性病例相比，HCV血清阳性患者更常与手术史相关(20% vs。3.9%;p = 0.044)，这与HCV阳性的几率显著增加相关（OR = 6.125, 95% CI: 1.221-30.719）。结论：本研究揭示了伊拉克库尔德斯坦地区血友病患者中HBV感染率中等（7.7%），HCV感染率较高（12.8%）。

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引用次数: 0

A systematic review on the correlation between COVID-19 and lower urinary tract symptoms COVID-19与下尿路症状相关性的系统综述

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2025.100202

Ashkan Shafigh , Amir Mohammadi-Garebagh , Kavous Shahsavarinia , Sona Tayebi , Ali Mostafaei , Hanieh Salehi-Pourmehr , Sakineh Hajebrahimi

COVID-19 can lead to extrapulmonary symptoms such as Lower Urinary Tract Symptoms (LUTS). We aimed to investigate the impact of the severity of SARS-CoV-2 infection on the lower urinary tract in patients affected by COVID-19. We conducted a comprehensive literature search using the terms "COVID-19," "SARS-CoV-2," and "Lower Urinary Tract Symptoms" with various combinations in MEDLINE, Scopus, ProQuest, Web of Science, Google Scholar, and Cochrane Library, including Cochrane Central Register of Controlled Trials (CENTRAL) and Cochrane Database of Systematic Reviews (CDSR) databases. The studies were selected based on eligibility criteria, and quantitative data were extracted using the data extraction tool from JBI-MAStARI. A total of 988 articles were found through the literature search. Twenty-five articles were included in our qualitative evaluation, and seven studies were included in the quantitative analysis. The qualitative publications were systematically reviewed separately under the titles of LUTS, benign prostatic hyperplasia (BPH), and kidney failures or kidney transplant recipients. The analysis of eligible studies showed a 3.3 % prevalence of LUTS in infected patients (95 % CI: 2.0 % - 5.3 %; Q-value: 1021.397, I2: 97.45 %). Furthermore, frequency and urgency were the most prevalent symptoms in the eligible meta-analysis studies, with 15.3 % (95 % CI: 5.7 % – 34.9 % in 4 studies) and 11.5 % (95 % CI: 7.1 % - 18.1 % in two studies), respectively. The prevalence of LUTS among COVID-19 patients was 3.3 %, with common symptoms including urinary frequency, urgency, UTI, and hematuria. Long-term follow-up and consideration of pre-existing LUTS are essential for improving understanding and clinical management.

COVID-19可导致肺外症状，如下尿路症状（LUTS）。我们旨在探讨SARS-CoV-2感染严重程度对COVID-19患者下尿路的影响。我们在MEDLINE、Scopus、ProQuest、Web of Science、谷歌Scholar和Cochrane图书馆(包括Cochrane中央对照试验注册库（Central）和Cochrane系统评价数据库（CDSR）数据库中使用“COVID-19”、“SARS-CoV-2”和“下尿路症状”等不同组合进行了全面的文献检索。根据入选标准选择研究，并使用JBI-MAStARI的数据提取工具提取定量数据。通过文献检索共找到988篇文章。我们的定性评价纳入了25篇文章，定量分析纳入了7篇研究。定性出版物分别在LUTS，良性前列腺增生（BPH）和肾衰竭或肾移植受者的标题下进行系统审查。对符合条件的研究的分析显示，感染患者中LUTS的患病率为3.3% (95% CI: 2.0% - 5.3%；q值：1021.397,I2: 97.45%)。此外，在符合条件的meta分析研究中，频率和急迫性是最常见的症状，分别为15.3%（4项研究中95% CI: 5.7% - 34.9%）和11.5%（2项研究中95% CI: 7.1% - 18.1%）。COVID-19患者中LUTS的患病率为3.3%，常见症状包括尿频、尿急、尿路感染和血尿。长期随访和考虑预先存在的LUTS是提高认识和临床管理的必要条件。

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引用次数: 0

Serum levels of the Parkinson's disease-linked protein Parkin are specifically elevated in COVID-19 在COVID-19中，帕金森病相关蛋白帕金森氏蛋白的血清水平特异性升高

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus

Pub Date : 2025-02-01 DOI: 10.1016/j.jcvp.2025.100206

Nadezhda G. Gumanova, Natalya L. Bogdanova, Alexander Yu. Gorshkov

Background

Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and is known to cause various unfavorable effects, including neurodegenerative disorders. The aim of the present study was to assess comparative serum protein profiles specifically associated with COVID-19.

Methods

COVID-19 infection was confirmed by the detection of immunoglobulin G (IgG)-SARS antibodies against SARS-CoV-2 S1 protein receptor-binding domain in serum samples collected in 2019–2021. IgG antibodies to adenovirus (IgG-AdV) were analyzed in serum samples collected in 2016–2018 prior the onset of the COVID-19 pandemic. Comparative protein profiling was conducted in matched serum samples with positive or negative IgG-SARS-status using the Signaling Explorer Antibody Array (SET100) that included 1358 specific antibodies in two replicates. This analysis identified Parkin as a top protein discriminating between the SARS-positive and negative status. The results were validated using in-house ELISA in the serum of participants recruited in 2019- 2021. Specificity versus another viral infection was tested in the serum samples with positive or negative status of IgG-AdV antibodies.

Results

High serum levels of the Parkinson's disease-linked protein Parkin were associated with SARS-positive status (P < 0.05) but were not associated with IgG-AdV-positive-status (P > 0.05).

Conclusions

Specific associations of Parkin with COVID-19 contribute to identification of the signaling pathways linked to COVID-19 effects.

冠状病毒病2019 （COVID-19）是由严重急性呼吸综合征冠状病毒2 （SARS-CoV-2）引起的，已知会导致各种不利影响，包括神经退行性疾病。本研究的目的是评估与COVID-19特异性相关的比较血清蛋白谱。方法检测2019-2021年采集的患者血清中针对SARS-CoV-2 S1蛋白受体结合域的免疫球蛋白G (IgG)-SARS抗体，确认感染。分析了2016-2018年COVID-19大流行发病前采集的血清样本中腺病毒IgG抗体（IgG- adv）。在igg - sars阳性或阴性的匹配血清样本中，使用包括两个重复的1358种特异性抗体的Signaling Explorer Antibody Array （SET100）进行比较蛋白谱分析。该分析确定Parkin是区分sars阳性和阴性状态的顶级蛋白。在2019- 2021年招募的参与者的血清中使用内部ELISA对结果进行了验证。在IgG-AdV抗体阳性或阴性的血清样本中检测对另一种病毒感染的特异性。结果血清中高水平的帕金森病相关蛋白Parkin与sars阳性状态相关(P <；0.05)，但与igg - adv阳性状态无关(P >；0.05)。结论帕金蛋白与COVID-19的特异性关联有助于识别与COVID-19效应相关的信号通路。

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