A bioanalytical method which utilizes high performance liquid chromatography with mass spectrometry method has been developed and validated for the quantification of valsartan in human plasma. The samples were processed by precipitation with formic acid then extracted with diethyl ether. Benazepril was used as an internal standard. The chromatographic separation is performed through C18 column with a mobile phase consisting of deionized water, acetonitrile and formic acid, followed by mass spectrometric detection in the positive ionization mode. The proposed method was specific and had been validated in the linear range of 50.0 – 5000.0 ng/ml for valsartan. The validation results were as follows: the intra-day and inter-day precision were 3.46 to 8.33% and 5.85 to 7.05% respectively, the intra-day and inter-day accuracy were 93.53 to 107.13% and 95.26 to 104.0% respectively. The recovery for valsartan and benazepril was 81.4% and 113.7% respectively. Also, stability was studied and the results obtained for short-term stability 99.24 to 102.32%, for freeze / thaw stability 99.75 to 99.95% and for long-term stability 98.24 to 103.03%. It can be concluded that the method can be applied in pharmacokinetic bioequivalence studies.
{"title":"Development and validation of bioanalytical method for the determination of valsartan in human plasma","authors":"S. H. Ramadhan","doi":"10.32947/ajps.v21i4.795","DOIUrl":"https://doi.org/10.32947/ajps.v21i4.795","url":null,"abstract":"A bioanalytical method which utilizes high performance liquid chromatography with mass spectrometry method has been developed and validated for the quantification of valsartan in human plasma. The samples were processed by precipitation with formic acid then extracted with diethyl ether. Benazepril \u0000 \u0000was used as an internal standard. The chromatographic separation is performed through C18 column with a mobile phase consisting of deionized water, acetonitrile and formic acid, followed by mass spectrometric detection in the positive ionization mode. The proposed method was specific and had been validated in the linear range of 50.0 – 5000.0 ng/ml for valsartan. The validation results were as follows: the intra-day and inter-day precision were 3.46 to 8.33% and 5.85 to 7.05% respectively, the intra-day and inter-day accuracy were 93.53 to 107.13% and 95.26 to 104.0% respectively. The recovery for valsartan and benazepril was 81.4% and 113.7% respectively. Also, stability was studied and the results obtained for short-term stability 99.24 to 102.32%, for freeze / thaw stability 99.75 to 99.95% and for long-term stability 98.24 to 103.03%. It can be concluded that the method can be applied in pharmacokinetic bioequivalence studies.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88834522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This work aims to enhance dissolution rate, increase absorption and bioavailability of Apigenin by formulating it as nanocrystal suspension employing ultrasonic technology. Two different diluents; (5%, 10%) microcrystalline cellulose (MCC) and (2%, 3%) anhydrous lactose were used to prepare hard gelatin capsules for two optimum types for apigenin nanocrystals prepared in our laboratory utilizing ultrasonication technique using 1% tween 80 (F6) and 2% poloxamer 188 (F20). The results showed that the marketed capsules (containing MCC) had about half the dissolution rate than all the prepared nanocrystals capsules formulas, and the nanocrystals prepared with poloxamer gave 90% release within 20 minutes and 100% release with 2 hours with excellent flow properties with no effect of the added diluents while the addition of diluents improved significantly the release of nanocrystals capsules (F6) with 1% tween 80 with good flow properties. The results suggested that utilizing apigenin nanocrystals prepared by ultrasonication technique may improve drug absorption and bioavailability with a reduced required dose.
{"title":"Preparation and evaluation of oral capsules containing apigenin nanocrystals prepared by ultrasonication","authors":"Mahmood A. Haiss, Nidhal K. Maraie","doi":"10.32947/ajps.v21i3.793","DOIUrl":"https://doi.org/10.32947/ajps.v21i3.793","url":null,"abstract":"This work aims to enhance dissolution rate, increase absorption and bioavailability of Apigenin by formulating it as nanocrystal suspension employing ultrasonic technology. Two different diluents; (5%, 10%) microcrystalline cellulose (MCC) and (2%, 3%) anhydrous lactose were used to prepare hard gelatin \u0000capsules for two optimum types for apigenin nanocrystals prepared in our laboratory utilizing ultrasonication technique using 1% tween 80 (F6) and 2% poloxamer 188 (F20). The results showed that the marketed capsules (containing MCC) had about half the dissolution rate than all the prepared nanocrystals capsules formulas, and the nanocrystals prepared with poloxamer gave 90% release within 20 minutes and 100% release with 2 hours with excellent flow properties with no effect of the added diluents while the addition of diluents improved significantly the release of nanocrystals capsules (F6) with 1% tween 80 with good flow properties. The results suggested that utilizing apigenin nanocrystals prepared by ultrasonication technique may improve drug absorption and bioavailability with a reduced required dose.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81426730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Multiple Sclerosis is a harmful disease causes severe and painful symptoms. In the recent period, researchers have tended to study the effect of vitamin D deficiency on general health. Several studies have reported that there is a relation between vitamin D levels and MS disease progression. Epidemiological testimony and reports have recorded that there are an association between the decrement in plasma vitamin D levels and the incidence of MS also with the disease development; and it has seen that when take vitamin D as supplement may protect the MS patients against disease development. Despite clear evidence of the correlation between disease progression and vitamin D deficiency but the mechanism yet unclear.
{"title":"The Impact of Vitamin D on The Development of Multiple Sclerosis (Review article)","authors":"Aseel Ghassan Daoud, Huda Jaber Waheed, Mayssaa Essam Abdala","doi":"10.32947/ajps.v21i3.792","DOIUrl":"https://doi.org/10.32947/ajps.v21i3.792","url":null,"abstract":"Multiple Sclerosis is a harmful disease causes severe and painful symptoms. In the recent period, researchers have tended to study the effect of vitamin D deficiency on general health. Several studies have reported that there is a relation between vitamin D levels and MS disease progression. Epidemiological testimony \u0000and reports have recorded that there are an association between the decrement in plasma vitamin D levels and the incidence of MS also with the disease development; and it has seen that when take vitamin D as supplement may protect the MS patients against disease development. Despite clear evidence of the correlation between disease progression and vitamin D deficiency but the mechanism yet unclear.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86565136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Noor S Jaafar, Maha N Hamad, Duha A Alshammaa, Zainab S Noori
Ficusreligiosa Linn, (Moraceae), is a large evergreen or deciduous, irregularly shaped tree. Traditionally the leaves are used for the treatment of constipation, vomiting, hiccup, and others. Leaves were extracted by two methods; maceration and soxhelt using hexane and 80% aqueous methanol, then subjected to preliminary phytochemical examination, fractionation with chloroform, ethyl acetate, and n.butanol, then TLC. Soxhelt was the suitable extraction method. Sterols, alkaloids, saponins, tannins, and flavonoids were identified in leaves. TLC examination demonstrates the possible presence of stigmasterol, chlorogenic acid, caffeic acid, rutin, and luteolin or apigenin.
{"title":"Phytochemical study and thin layer chromatography of Ficusreligiosa leaves extract cultivated in Iraq","authors":"Noor S Jaafar, Maha N Hamad, Duha A Alshammaa, Zainab S Noori","doi":"10.32947/ajps.v21i2.807","DOIUrl":"https://doi.org/10.32947/ajps.v21i2.807","url":null,"abstract":"Ficusreligiosa Linn, (Moraceae), is a large evergreen or deciduous, irregularly shaped tree. Traditionally the leaves are used for the treatment of constipation, vomiting, hiccup, and others. Leaves were extracted by two methods; maceration and soxhelt using hexane and \u0000 \u000080% aqueous methanol, then subjected to preliminary phytochemical examination, fractionation with chloroform, ethyl acetate, and n.butanol, then TLC. Soxhelt was the suitable extraction method. Sterols, alkaloids, saponins, tannins, and flavonoids were identified in leaves. TLC examination demonstrates the possible presence of stigmasterol, chlorogenic acid, caffeic acid, rutin, and luteolin or apigenin.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85293830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Israa Burhan Raoof, Zahraa Ahmed Okhti, Mayssaa E. Abdalah
Covid -19 is a viral disease play important role in danger pathogens for human, it is infecting on respiratory, hepatic, central nervous systems and gastrointestinal, in addition to that, the triggers included cytokine storm is inflammatory response to viral infection Therefore, excessive enhance of immune cells lead to new of pro-inflammatory cytokines. COVID-19 interaction with the cardiovascular system on different levels, rising death percent in people underlying cardiovascular state provoking myocardial injury. Respiratory infection varying from mild cold to the severe illness like as Middle East Respiratory Syndrome and Severe Acute Respiratory Syndrome the most important coronavirus (COVID-19) complications, however individuals with asymptomatic disease were also suspected of possible infectious transmission, which further adds to the uncertainty of the dynamic of disease transmission in COVID-19 infections, Suppression of angiotensin-converting enzyme (ACE2) expression by inflammatory cytokines accompanied by the decrease of estrogens and androgens of the elderly, establish a negative correlation between ACE2 expression and mortality in other cases may lead to infertility. Early detection by PCR has aided the identification of the pathogen at an early level. Furthermore, the treatment includes the effectiveness of glucocorticoid drugs (dexamethasone), anti-malaria drug (hydroxychloroquine), azithromycin (antibacterial drug) and favipiravir are all drugs recommended in illness with Covid-19. Remdesivir suppression COVID-19 replication and decreases viral load, alleviates clinical signs and improves pulmonary injury, therefore Remdesivir drug has been used as a compassionate medicine for treating COVID-19 patients.
{"title":"Clinical Signs, Laboratory Diagnosis and Treatments Involved in Corona Viruses-19","authors":"Israa Burhan Raoof, Zahraa Ahmed Okhti, Mayssaa E. Abdalah","doi":"10.32947/ajps.v21i1.776","DOIUrl":"https://doi.org/10.32947/ajps.v21i1.776","url":null,"abstract":"Covid -19 is a viral disease play important role in danger pathogens for human, it is infecting on respiratory, hepatic, central nervous systems and gastrointestinal, in addition to that, the triggers included cytokine storm is inflammatory response to viral infection \u0000 \u0000 \u0000Therefore, excessive enhance of immune cells lead to new of pro-inflammatory cytokines. COVID-19 interaction with the cardiovascular system on different levels, rising death percent in people underlying cardiovascular state provoking myocardial injury. Respiratory infection varying from mild cold to the severe illness like as Middle East Respiratory Syndrome and Severe Acute Respiratory Syndrome the most important coronavirus (COVID-19) complications, however individuals with asymptomatic disease were also suspected of possible infectious transmission, which further adds to the uncertainty of the dynamic of disease transmission in COVID-19 infections, Suppression of angiotensin-converting enzyme (ACE2) expression by inflammatory cytokines accompanied by the decrease of estrogens and androgens of the elderly, establish a negative correlation between ACE2 expression and mortality in other cases may lead to infertility. Early detection by PCR has aided the identification of the pathogen at an early level. Furthermore, the treatment includes the effectiveness of glucocorticoid drugs (dexamethasone), anti-malaria drug (hydroxychloroquine), azithromycin (antibacterial drug) and favipiravir are all drugs recommended in illness with Covid-19. Remdesivir suppression COVID-19 replication and decreases viral load, alleviates clinical signs and improves pulmonary injury, therefore Remdesivir drug has been used as a compassionate medicine for treating COVID-19 patients.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85531839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mustafa Hameed Majeed, Abdul–Kareem Kadhim Alkhazraji
Background: Bell’s palsy is unilateral peripheral paralysis of the seventh cranial nerve, several mechanisms have been proposed in the pathogenesis of this disease, among the viral infections specially herpes virus's family including roseola viruses that have been detected in patients' saliva. Method: A case-control study conducted on Bell’s palsy patients at al-Imamein Al-kadhimein Medical City throughout the period from March 2019 to November 2019. Saliva samples were taken from 50 patients (18-55) years of age with early stages of Bell's palsy and from 50 apparently healthy and age and sex matched volunteers as control. Viral DNA was extracted from the saliva and then real time PCR for detection and quantification of HHV6 DNA in these patients Results: Saliva samples taken from 28 patients in the Bell’s palsy patients (56%) tested positive for HHV6, as vs. to just 8 (16%) in the healthy group(control) (P= 0.028). There was increase amount of HHV6 load in patients compare to control group (p=0.001). Regarding the demographic values, an important difference in the detection rate for Human herpes virus-6 between male (64%) and females (35%) (P= 0.02). There was significant correlation between age and grading (p =0.01), as increase age correlate with high grading, also between viral load of HH6 and grading as increase viral load of the virus correlate with high grading (p = 0.001). Conclusion: HHV-6 virus might have a potential role in the pathogenesis of Bell’s palsy.
{"title":"Detection of Human Herpes Virus-6 in saliva of Patients with Bell's palsy","authors":"Mustafa Hameed Majeed, Abdul–Kareem Kadhim Alkhazraji","doi":"10.32947/ajps.v21i1.801","DOIUrl":"https://doi.org/10.32947/ajps.v21i1.801","url":null,"abstract":"Background: Bell’s palsy is unilateral peripheral paralysis of the seventh cranial nerve, several mechanisms have been proposed in the pathogenesis of this disease, among the viral infections specially herpes virus's family including roseola viruses that have been detected in patients' saliva. \u0000Method: A case-control study conducted on Bell’s palsy patients at al-Imamein Al-kadhimein Medical City throughout the period from March 2019 to November 2019. Saliva samples were taken from 50 patients (18-55) years of age with early stages of Bell's palsy and from 50 apparently healthy and age and sex matched volunteers as control. Viral DNA was extracted from the saliva and then real time PCR for detection and quantification of HHV6 DNA in these patients \u0000Results: Saliva samples taken from 28 patients in the Bell’s palsy patients (56%) tested positive for HHV6, as vs. to just 8 (16%) in the healthy group(control) (P= 0.028). There was increase amount of HHV6 load in patients compare to control group (p=0.001). Regarding the demographic values, an important difference in the detection rate for Human herpes virus-6 between male (64%) and females (35%) (P= 0.02). There was significant correlation between age and grading (p =0.01), as increase age correlate with high grading, also between viral load of HH6 and grading as increase viral load of the virus correlate with high grading (p = 0.001). \u0000Conclusion: HHV-6 virus might have a potential role in the pathogenesis of Bell’s palsy.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85108038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Polymeric micelles are nanoscale core-shell structures formed by amphiphilic (block or graft) copolymers, that can self-aggregate in an aqueous medium. PMs characterized by small size, spherical shape, lower critical micellar concentration, which gave higher stability for PMs over conventional surfactant micelles. The core/shell structure permits polymeric micelle to entrap poor soluble drugs and can improve their solubility and permeability. The preparation of PMs tends to be relatively easy as compared to other novel drug delivery systems. This review focus on the general properties, types, types of copolymer utilized, formation mechanism, preparation methods, characterization techniques, and the applications on PMs.
{"title":"Highlights on polymeric micelles as versatile nanocarriers for drug transporting","authors":"Hussein A. Abdul Hussein, Nidhal K. Maraie","doi":"10.32947/ajps.v21i2.806","DOIUrl":"https://doi.org/10.32947/ajps.v21i2.806","url":null,"abstract":"Polymeric micelles are nanoscale core-shell structures formed by amphiphilic (block or graft) copolymers, that can self-aggregate in an aqueous medium. PMs characterized by small size, spherical shape, lower critical micellar concentration, which gave higher \u0000stability for PMs over conventional surfactant micelles. The core/shell structure permits polymeric micelle to entrap poor soluble drugs and can improve their solubility and permeability. The preparation of PMs tends to be relatively easy as compared to other novel drug delivery systems. This review focus on the general properties, types, types of copolymer utilized, formation mechanism, preparation methods, characterization techniques, and the applications on PMs.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89605742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fatima Mohammad Saeed Mahdi, M. G. Alabbassi, Muthanna Ibrahim, Al-Ezzi
Fibrosis of the lungs is the final phase of many lung illnesses. Its characterized by excessive matrix production leading to the normal lung architecture destruction and eventually death. CoQ10 is an essential constituent of membrane oxidoreductase System. It is an intracellularly localized antioxidant enzyme that is endogenously synthesized in humans. CoQ10 protects cellular components from destruction by free radical-induced oxidative damage. candesartan is a selective antagonist of angiotensin II type 1 receptor (AT1) and is widely employed for treatment of hypertension. Most up to date research shows angiotensin II close involvement with damage and fibrosis process in tissues of organs of circulation. Objective: This animal study was designed to investigate the effect of Co Q10 and candesartan as antifibrotic agents against pulmonary fibrosis focusing on selected markers involved in pulmonary fibrosis. Materials and Method: 48 rats divided randomly into four groups, each consisting of 12 male rats. Group I: (Control group), the rats in this group received single injection of 0.2 ml normal saline via intratracheal route. Group II: (The BLM group), received BLM (8.3 U/kg) as a single dose via the intratracheal rout. Group III: (BLM+CoQ10 group) rats receive BLM (8.3 U/kg) as sulfate salt dissolved in 0.1 ml of normal saline via the intratracheal instillation concomitant with 100 mg/kg co Q 10 per day orally for 5 days before and 10 days after BLM injection. Group IV: (BLM+ candesartan group) rats receive BLM (8.3 U/kg) as sulfate salt dissolved in 0.1 ml of normal saline via the intratracheal instillation concomitant with (10 mg/kg) candesartan per day orally for 5 days before and 10 days after BLM injection. The studied serum biomarkers were glutathione, arachidonate 5 lipoxygenase in addition to histopathological examination using trichrome stain. Results: serum GSH concentration was lower in BLM group in comparison with the control group and the (BLM+ CoQ10) but this difference is not statistically significant. The serum GSH levels showed a significant(P<0.05) elevation in (BLM+ Candesartan) group when compared with the levels in the BLM group. The serum ALOX5 concentration was significantly elevated in BLM group in comparison with the control group (P < 0.05). The serum ALOX5 levels were highly significantly lower in (BLM +CoQ10) (P < 0.01) and very highly significantly lower in the (BLM+ Candesartan) group (P < 0.001) when comparing it with the levels in the BLM group. furthermore, histologically CoQ10 and candesartan showed reduction in the numbers of inflammation cells and a decrease in the damage to the lung architecture and fibrosis induced by bleomycin. Conclusion: CoQ10 and candesartan decrease pulmonary fibrosis induced by bleomycin in male rats.
{"title":"Protective Effect of Co Q10 and Candesartan on Bleomyycin Induced Lung Fibrosis in Rats","authors":"Fatima Mohammad Saeed Mahdi, M. G. Alabbassi, Muthanna Ibrahim, Al-Ezzi","doi":"10.32947/ajps.v21i1.787","DOIUrl":"https://doi.org/10.32947/ajps.v21i1.787","url":null,"abstract":"Fibrosis of the lungs is the final phase of many lung illnesses. Its characterized by excessive matrix production leading to the normal lung architecture destruction and eventually death. CoQ10 is an essential constituent of membrane oxidoreductase System. It is an intracellularly localized antioxidant \u0000 \u0000enzyme that is endogenously synthesized in humans. CoQ10 protects cellular components from destruction by free radical-induced oxidative damage. candesartan is a selective antagonist of angiotensin II type 1 receptor (AT1) and is widely employed for treatment of hypertension. Most up to date research shows angiotensin II close involvement with damage and fibrosis process in tissues of organs of circulation. \u0000Objective: This animal study was designed to investigate the effect of Co Q10 and candesartan as antifibrotic agents against pulmonary fibrosis focusing on selected markers involved in pulmonary fibrosis. \u0000Materials and Method: 48 rats divided randomly into four groups, each consisting of 12 male rats. Group I: (Control group), the rats in this group received single injection of 0.2 ml normal saline via intratracheal route. Group II: (The BLM group), received BLM (8.3 U/kg) as a single dose via the intratracheal rout. Group III: (BLM+CoQ10 group) rats receive BLM (8.3 U/kg) as sulfate salt dissolved in 0.1 ml of normal saline via the intratracheal instillation concomitant with 100 mg/kg co Q 10 per day orally for 5 days before and 10 days after BLM injection. Group IV: (BLM+ candesartan group) rats receive BLM (8.3 U/kg) as sulfate salt dissolved in 0.1 ml of normal saline via the intratracheal instillation concomitant with (10 mg/kg) candesartan per day orally for 5 days before and 10 days after BLM injection. The studied serum biomarkers were glutathione, arachidonate 5 lipoxygenase in addition to histopathological examination using trichrome stain. \u0000 Results: serum GSH concentration was lower in BLM group in comparison with the control group and the (BLM+ CoQ10) but this difference is not statistically significant. The serum GSH levels showed a significant(P<0.05) elevation in (BLM+ Candesartan) group when compared with the levels in the BLM group. The serum ALOX5 concentration was significantly elevated in BLM group in comparison with the control group (P < 0.05). The serum ALOX5 levels were highly significantly lower in (BLM +CoQ10) (P < 0.01) and very highly significantly lower in the (BLM+ Candesartan) group (P < 0.001) when comparing it with the levels in the BLM group. furthermore, histologically CoQ10 and candesartan showed reduction in the numbers of inflammation cells and a decrease in the damage to the lung architecture and fibrosis induced by bleomycin. \u0000Conclusion: CoQ10 and candesartan decrease pulmonary fibrosis induced by bleomycin in male rats.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90698124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mazin Abdulridha Ateyah, Manal Khalid Abdulridha, Munaim Jumaa Alkabee
Background Saw palmetto contains powerful anti-inflammatory components commonly used in Benign prostatic hyperplasia (BPH) patients with symptoms. The current study was designed to assess the effectiveness of saw palmetto alone or supplementation therapy with tamsulosin via measurement of some inflammatory biomarker in male with lower urinary tract symptoms (LUTS) consistent with (BPH). Methods The present study is an interventional prospective randomized enrolled newly diagnosed patients with moderate to severe symptomatic BPH. The eligible 60 patients were allocated into (3) groups, each group with (20) patients; Group (1) treated with Saw palmetto cap (320 mg); Group (2) treated with Saw palmetto cap (320 mg) and tamsulosin (0.4mg); Group (3) treated with tamsulosin (0.4mg), protocols to be given once daily for 12 weeks. Measurement of prostate specific antigen (PSA), C-reactive protein (CRP), and interleukin 6 (IL-6) was done. Results There was significant decrease in the serum PSA, CRP and IL-6 level (P<0.01) in group1 and 2 patients only after treatment when compared to pretreatment. Patients on combination therapy presented with high percent of change compared to other groups. No change in CRP and IL-6 level in patients on tamsulosin alone. Conclusion From the findings of the present study, phytotherapy with Saw Palmetto alone or as supplement produced significant 3 months drop in both inflammatory markers, the total CRP level and IL-6 level. Also produced significant decrease in PSA level, and may substitute the conventional therapy in mild disease status.
{"title":"Effects of Saw Palmetto Therapy on some Inflammatory Biomarkers in a Sample of Iraqi Male with Symptomatic Benign Prostatic Hyperplasia","authors":"Mazin Abdulridha Ateyah, Manal Khalid Abdulridha, Munaim Jumaa Alkabee","doi":"10.32947/ajps.v21i1.774","DOIUrl":"https://doi.org/10.32947/ajps.v21i1.774","url":null,"abstract":"Background Saw palmetto contains powerful anti-inflammatory components commonly used in Benign prostatic hyperplasia (BPH) patients with symptoms. The current study was designed to assess the effectiveness of saw palmetto alone or supplementation \u0000 \u0000therapy with tamsulosin via measurement of some inflammatory biomarker in male with lower urinary tract symptoms (LUTS) consistent with (BPH). \u0000Methods The present study is an interventional prospective randomized enrolled newly diagnosed patients with moderate to severe symptomatic BPH. The eligible 60 patients were allocated into (3) groups, each group with (20) patients; Group (1) treated with Saw palmetto cap (320 mg); Group (2) treated with Saw palmetto cap (320 mg) and tamsulosin (0.4mg); Group (3) treated with tamsulosin (0.4mg), protocols to be given once daily for 12 weeks. Measurement of prostate specific antigen (PSA), C-reactive protein (CRP), and interleukin 6 (IL-6) was done. \u0000Results There was significant decrease in the serum PSA, CRP and IL-6 level (P<0.01) in group1 and 2 patients only after treatment when compared to pretreatment. Patients on combination therapy presented with high percent of change compared to other groups. No change in CRP and IL-6 level in patients on tamsulosin alone. \u0000Conclusion From the findings of the present study, phytotherapy with Saw Palmetto alone or as supplement produced significant 3 months drop in both inflammatory markers, the total CRP level and IL-6 level. Also produced significant decrease in PSA level, and may substitute the conventional therapy in mild disease status.","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76131221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Taha HT Al-Saigh, Shatha A Abdulmawjood, Faris A Ahmed
Colorectal cancer is a serious cancer with high mortality. Most of the recurrence usually occurred within two years after surgery. This study was conducted in Nineveh Medical Center and Al-Jammhorri Hospital to evaluate colorectal cancer patients in a follow up study for two years. Serum carcinoembryonic antigen, colonoscopy, ultrasound of the abdomen, and chest X rays were used for evaluation. One hundred and ninety-four patients with colorectal cancer were included in the study. The patients were diagnosed colorectal adenocarcinomas by histopathology and staged according to Duke’s classification. The patients were undergone surgical removal of the cancer. Chemotherapy was started to the patient after two weeks of the surgery. Blood samples were taken one week before and one week after surgery. Other blood samples were taken after chemotherapy and then every six months for two years. The blood samples were analyzed for serum carcinoembryonic antigen. The patients were also checked by colonoscopy, ultrasound for abdomen, and chest X ray every six months. Serum carcinoembryonic antigen in colorectal patients was higher than 5 ng/mL and decreased significantly after surgery but still higher than 5 ng/mL. After chemotherapy serum decreased significantly compared with that after surgery. During the two-year period serum carcinoembryonic antigen was not significantly different from that in patients after chemotherapy. Twenty-one patients died after two years of the study Serum carcinoembryonic antigen in the dead patients was much significantly higher than 5 ng/mL before surgery and decreased significantly after operation and after chemotherapy but still higher than 5 ng/mL. In conclusion, colorectal cancer is a significant disease in Iraq. The mortality rate is high due to lack of education of the community to that disease. Carcinoembryonic antigen marker is still acceptable test but should be used with other clinical assessments
{"title":"Prognostic factor of serum carcinoembryonic antigen in colorectal cancer patients: a follow up study","authors":"Taha HT Al-Saigh, Shatha A Abdulmawjood, Faris A Ahmed","doi":"10.32947/ajps.v21i3.791","DOIUrl":"https://doi.org/10.32947/ajps.v21i3.791","url":null,"abstract":"Colorectal cancer is a serious cancer with high mortality. Most of the recurrence usually occurred within two years after surgery. This study was conducted in Nineveh Medical Center and Al-Jammhorri Hospital to evaluate colorectal cancer patients in a follow up study for \u0000two years. Serum carcinoembryonic antigen, colonoscopy, ultrasound of the abdomen, and chest X rays were used for evaluation. One hundred and ninety-four patients with colorectal cancer were included in the study. The patients were diagnosed colorectal adenocarcinomas by histopathology and staged according to Duke’s classification. The patients were undergone surgical removal of the cancer. Chemotherapy was started to the patient after two weeks of the surgery. Blood samples were taken one week before and one week after surgery. Other blood samples were taken after chemotherapy and then every six months for two years. The blood samples were analyzed for serum carcinoembryonic antigen. The patients were also checked by colonoscopy, ultrasound for abdomen, and chest X ray every six months. Serum carcinoembryonic antigen in colorectal patients was higher than 5 ng/mL and decreased significantly after surgery but still higher than 5 ng/mL. After chemotherapy serum decreased significantly compared with that after surgery. During the two-year period serum carcinoembryonic antigen was not significantly different from that in patients after chemotherapy. Twenty-one patients died after two years of the study Serum carcinoembryonic antigen in the dead patients was much significantly higher than 5 ng/mL before surgery and decreased significantly after operation and after chemotherapy but still higher than 5 ng/mL. In conclusion, colorectal cancer is a significant disease in Iraq. The mortality rate is high due to lack of education of the community to that disease. Carcinoembryonic antigen marker is still acceptable test but should be used with other clinical assessments","PeriodicalId":7406,"journal":{"name":"Al Mustansiriyah Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82439525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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