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Care Literacy for Culture, Nature, and Future 文化、自然和未来的关爱素养
Pub Date : 2024-06-13 DOI: 10.12688/openreseurope.17817.1
Hiroko Costantini, Misato Nihei, Masakazu Sugiyama, Nobuyuki Yagi, James Costantini
Within the broader sustainability agenda, an important element relates to the need for a transformative approach to nature. This motivates and is reflected in the Natures Futures Framework. Within this framework, this letter focuses on the relational value of Nature as Culture/One with Nature. This is important yet complex as part of the re-orienting of values to enable truly significant change, and which necessitates individual and community involvement on the value of caring for nature. As a means for understanding and enabling individuals’ potential to engage and contribute, the notion of ‘care for nature literacy’ is put forward.
在更广泛的可持续发展议程中,一个重要因素是需要对自然采取变革性的方法。这也是 "自然未来框架 "的动机和体现。在此框架内,本信重点关注 "自然作为文化/与自然合一 "的关系价值。这一点既重要又复杂,是重新定位价值观以实现真正重大变革的一部分,需要个人和社区参与到关爱自然的价值观中来。作为理解和发挥个人参与和贡献潜能的一种手段,我们提出了 "关爱自然素养 "的概念。
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引用次数: 0
Understanding Syrian migration in Lebanon: a methodological framework. 了解黎巴嫩境内的叙利亚移民:方法框架。
Pub Date : 2024-06-13 eCollection Date: 2023-01-01 DOI: 10.12688/openreseurope.16261.2
Maria Gabriella Trovato, Nayla Al-Akl, Dana Ali

Background: Wars, crises, and climate change are just a few of the worldwide concerns that have resulted in the forced relocation of millions of people. After 12 years of conflict in Syria, millions of Syrians are still displaced in the neighbouring countries, and their conditions have worsened due to the economic and socio-political crisis of the region. This paper reports on a study conducted in Lebanon as part of the EU Horizon-funded project ADMIGOV - Advancing Alternative Migration Governance. It describes the methodological framework used to study Syrian migration in Lebanon and sheds light on the phenomenon's patterns, challenges, and impacts.

Methods: In our study, we opted for a mixed method. It is built on a large corpus of primary data collected over the course of years of intensive, in-depth fieldwork and the author's immersion in the community. Alongside observations, quantitative and qualitative phone interviews were conducted to obtain the perceptions of displaced Syrians living in informal tented settlements in rural Lebanon and an incomplete building in the city of Saida. This interview data is accompanied by primary and secondary data sources, including the findings of other European research projects, statistics from UNHCR and IOM, and academic and press articles.

Results: Our research revealed the difficulties Syrians displaced in Lebanon encounter while navigating the challenging situation they are trapped in. Based on a case study approach, it unveils similarities and differences determined by the government's no encampment policy that led to self-settled practices across the country. This approach helped in understanding the challenging dynamic created by weak public institutions and their failure to guarantee the observance of basic human rights, compromising displaced Syrians safety. The weakness of public institutions and their failure to guarantee the observance of basic human rights has compromised displaced safety. Moreover, even though the development interventions and aid assistance have been necessary for Syrians' survival, they proved insufficient, and unequally distributed by location evidencing the inefficiencies of the majority of development aid projects.

Conclusions: The findings contribute to an enriched understanding of the situation of Syrians in Lebanon and offer insights for policymakers, practitioners, and researchers working in the field of forced migration and humanitarian responses.

背景:战争、危机和气候变化只是导致数百万人被迫迁移的全球问题中的一小部分。在叙利亚经历了 12 年的冲突之后,仍有数百万叙利亚人在邻国流离失所,而且由于该地区的经济和社会政治危机,他们的境况更加恶化。本文报告了在黎巴嫩开展的一项研究,该研究是欧盟 "地平线 "资助项目 "ADMIGOV--推进替代性移民治理 "的一部分。它描述了研究黎巴嫩境内叙利亚移民所使用的方法框架,并揭示了这一现象的模式、挑战和影响:在我们的研究中,我们选择了一种混合方法。它建立在作者多年深入实地考察和深入社区收集的大量原始数据基础之上。除了观察,我们还进行了定量和定性电话访谈,以了解居住在黎巴嫩农村非正式帐篷定居点和赛达市一栋不完整建筑中的流离失所叙利亚人的看法。这些访谈数据附有主要和次要数据来源,包括其他欧洲研究项目的结果、联合国难民署和国际移民组织的统计数据以及学术和报刊文章:我们的研究揭示了在黎巴嫩流离失所的叙利亚人在应对他们所处的严峻形势时遇到的困难。研究以案例研究法为基础,揭示了由政府的 "无营地政策 "所决定的全国各地自我安置做法的异同。这种方法有助于理解由于公共机构薄弱及其未能保障基本人权的遵守而造成的挑战性动态,从而损害了流离失所的叙利亚人的安全。公共机构薄弱,无法保证基本人权得到尊重,这损害了流离失所者的安全。此外,尽管发展干预和援助对叙利亚人的生存是必要的,但事实证明这些干预和援助是不够的,而且在不同地区的分布也不均衡,这证明了大多数发展援助项目的效率低下:研究结果有助于丰富对黎巴嫩境内叙利亚人状况的理解,并为被迫移民和人道主义应对领域的政策制定者、从业人员和研究人员提供了启示。
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引用次数: 0
An increase in global daily precipitation records in response to global warming based on reanalysis and observations 基于再分析和观测的全球日降水量记录随全球变暖而增加
Pub Date : 2024-06-10 DOI: 10.12688/openreseurope.17674.1
J. Ciarlo, Filippo Giorgi
Understanding trends in extreme precipitation events in the context of global warming is critical for assessing climate change impacts. This study employs a novel methodology developed by Giorgi and Ciarlo (2022) to analyze record-breaking daily precipitation events from 1980 to 2020, utilizing three reanalysis products (ERA5, MERRA-2, and JRA-55) and one global observation dataset (MSWEP). Our results indicate a consistent and statistically significant increase in record-breaking precipitation events globally, with variations across different latitude bands and between land and ocean areas. This trend is evident in all datasets, with the most substantial increases observed over oceans in ERA5 and over land in JRA and MERRA. Notably, the Southern Hemisphere shows mixed results, with some regions displaying negative trends. This study highlights the increasing frequency of extreme precipitation events, supporting the hypothesis of intensified hydrological cycles under a warming climate. Our findings enhance understanding of precipitation extremes and underscore the importance of regional analyses in climate impact studies. Future work could extend these findings to formal attribution studies linking observed trends directly to anthropogenic climate change.
了解全球变暖背景下极端降水事件的趋势对于评估气候变化的影响至关重要。本研究采用了 Giorgi 和 Ciarlo(2022 年)开发的一种新方法,利用三个再分析产品(ERA5、MERRA-2 和 JRA-55)和一个全球观测数据集(MSWEP),分析了 1980 年至 2020 年破纪录的日降水事件。我们的研究结果表明,全球破纪录的降水事件在统计意义上呈持续增长趋势,不同纬度带以及陆地和海洋地区之间存在差异。这一趋势在所有数据集中都很明显,ERA5 中海洋降水量增加最多,JRA 和 MERRA 中陆地降水量增加最多。值得注意的是,南半球的结果好坏参半,一些地区呈现负趋势。这项研究强调了极端降水事件发生频率的增加,支持气候变暖下水文循环加剧的假设。我们的研究结果加深了人们对极端降水的理解,并强调了气候影响研究中区域分析的重要性。未来的工作可以将这些发现扩展到正式的归因研究,将观测到的趋势与人为气候变化直接联系起来。
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引用次数: 0
Boundaries of the built environment: defining the significance of the material presence of spatial morphology in social life. 建筑环境的边界:界定空间形态的物质存在在社会生活中的意义。
Pub Date : 2024-06-10 eCollection Date: 2023-01-01 DOI: 10.12688/openreseurope.16591.2
Benjamin N Vis

Settled societies inhabit environments shaped by building activity. Geographic data in social scientific and geographical research are generally composed of architectural and social categories derived from commonplace lived experience and societal knowledge, thus carrying socio-culturally specific meaning. The mundane pragmatism of such categories conflate spaces and buildings with their use and may obstruct effective comparison. Here I introduce a set of formally redescriptive ontological concepts for built environments that operates on the basis of how differentiation and subdivision constitute distinct occupiable spaces through boundaries. An ontology of the inhabited built environment arises from the application of these socio-spatial and material concepts called 'Boundary Line Types' (BLT). I present and photographically illustrate the definitions of the BLTs, which are conceived on a critical realist basis and rooted in a multidisciplinary body of theory concerning the development and inhabitation of built space. Considering inhabited built environments through BLTs foregrounds the emergent logic by which spaces are divided and connected, creating configurations of boundaries as material frames that afford everyday social life. Since BLTs offer transferrable empirical principles from which these material frames emerge, they also enable diachronic and cross-cultural comparative social research. My proposition to approach social scientific built environment research through constitutive material boundaries offers a comparative complement to commonplace and socio-culturally specific spatial categories that compose most geographic data, enabling formal thick redescriptions and the potential for quantitative spatial analysis.

定居社会所居住的环境是由建筑活动所塑造的。社会科学和地理研究中的地理数据通常由建筑和社会类别组成,这些类别来自于普通的生活经验和社会知识,因此具有特定的社会文化含义。这些类别的世俗实用主义将空间和建筑与其用途混为一谈,可能会阻碍有效的比较。在此,我为建筑环境引入了一套形式上重新描述的本体论概念,其运作基础是区分和细分如何通过边界构成不同的可居住空间。通过应用这些被称为 "边界线类型"(BLT)的社会空间和物质概念,产生了一种居住建筑环境的本体论。我介绍并用图片说明了 "边界线类型 "的定义,这些定义是在批判现实主义的基础上提出的,植根于有关建筑空间发展和居住的多学科理论体系。通过BLTs考虑居住的建筑环境,可以预见空间被划分和连接的新兴逻辑,创造出作为物质框架的边界配置,从而提供日常的社会生活。由于 BLT 提供了可转移的经验原则,而这些物质框架正是由此产生的,因此 BLT 也有助于进行非同步和跨文化的比较社会研究。我提出的通过构成性物质边界来开展社会科学建筑环境研究的主张,为构成大多数地理数据的普通和社会文化特定空间类别提供了比较性补充,实现了正式的厚重再描述和定量空间分析的潜力。
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引用次数: 0
Large Language Models and OpenLogos: An Educational Case Scenario 大型语言模型和 OpenLogos:教育案例场景
Pub Date : 2024-06-05 DOI: 10.12688/openreseurope.17605.1
Andrijana Pavlova, B. Gerazov, Anabela Barreiro
Large Language Models (LLMs) offer advanced text generation capabilities, sometimes surpassing human abilities. However, their use without proper expertise poses significant challenges, particularly in educational contexts. This article explores different facets of natural language generation (NLG) within the educational realm, assessing its advantages and disadvantages, particularly concerning LLMs. It addresses concerns regarding the opacity of LLMs and the potential bias in their generated content, advocating for transparent solutions. Therefore, it examines the feasibility of integrating OpenLogos expert-crafted resources into language generation tools used for paraphrasing and translation. In the context of the Multi3Generation COST Action (CA18231), we have been emphasizing the significance of incorporating OpenLogos into language generation processes, and the need for clear guidelines and ethical standards in generative models involving multilingual, multimodal, and multitasking capabilities. The Multi3Generation initiative strives to progress NLG research for societal welfare, including its educational applications. It promotes inclusive models inspired by the Logos Model, prioritizing transparency, human control, preservation of language principles and meaning, and acknowledgment of the expertise of resource creators. We envision a scenario where OpenLogos can contribute significantly to inclusive AI-supported education. Ethical considerations and limitations related to AI implementation in education are explored, highlighting the importance of maintaining a balanced approach consistent with traditional educational principles. Ultimately, the article advocates for educators to adopt innovative tools and methodologies to foster dynamic learning environments that facilitate linguistic development and growth.
大型语言模型(LLM)具有先进的文本生成能力,有时甚至超越了人类的能力。然而,在没有适当专业知识的情况下使用它们会带来巨大挑战,尤其是在教育领域。本文探讨了教育领域中自然语言生成(NLG)的不同方面,评估了其优缺点,特别是有关 LLM 的优缺点。文章探讨了人们对 LLM 不透明及其生成内容中潜在偏见的担忧,提倡采用透明的解决方案。因此,它研究了将 OpenLogos 专家制作的资源整合到用于转述和翻译的语言生成工具中的可行性。在 Multi3Generation COST 行动(CA18231)的背景下,我们一直在强调将 OpenLogos 纳入语言生成过程的重要性,以及在涉及多语言、多模态和多任务处理能力的生成模型中制定明确指导原则和道德标准的必要性。Multi3Generation 计划致力于推进无语言生成技术研究,以造福社会,包括其教育应用。它提倡受 Logos 模型启发的包容性模型,优先考虑透明度、人为控制、语言原则和意义的保护,以及对资源创建者专业知识的认可。在我们的设想中,OpenLogos 可以为人工智能支持的包容性教育做出重大贡献。文章探讨了在教育领域实施人工智能的相关伦理考虑因素和限制,强调了保持与传统教育原则相一致的平衡方法的重要性。最后,文章倡导教育工作者采用创新的工具和方法,营造动态的学习环境,促进语言的发展和成长。
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引用次数: 0
Atacama Large Aperture Submillimeter Telescope (AtLAST) science: Our Galaxy. 阿塔卡马大孔径亚毫米波望远镜(ATLAST)科学:我们的银河系
Pub Date : 2024-06-05 eCollection Date: 2024-01-01 DOI: 10.12688/openreseurope.17450.1
Pamela Klaassen, Alessio Traficante, Maria Beltrán, Kate Pattle, Mark Booth, Joshua Lovell, Jonathan Marshall, Alvaro Hacar, Brandt Gaches, Caroline Bot, Nicolas Peretto, Thomas Stanke, Doris Arzoumanian, Ana Duarte Cabral, Gaspard Duchêne, David Eden, Antonio Hales, Jens Kauffmann, Patricia Luppe, Sebastian Marino, Elena Redaelli, Andrew Rigby, Álvaro Sánchez-Monge, Eugenio Schisano, Dmitry Semenov, Silvia Spezzano, Mark Thompson, Friedrich Wyrowski, Claudia Cicone, Tony Mroczkowski, Martin Cordiner, Luca Di Mascolo, Doug Johnstone, Eelco van Kampen, Minju Lee, Daizhong Liu, Thomas Maccarone, Amélie Saintonge, Matthew Smith, Alexander Thelen, Sven Wedemeyer

As we learn more about the multi-scale interstellar medium (ISM) of our Galaxy, we develop a greater understanding for the complex relationships between the large-scale diffuse gas and dust in Giant Molecular Clouds (GMCs), how it moves, how it is affected by the nearby massive stars, and which portions of those GMCs eventually collapse into star forming regions. The complex interactions of those gas, dust and stellar populations form what has come to be known as the ecology of our Galaxy. Because we are deeply embedded in the plane of our Galaxy, it takes up a significant fraction of the sky, with complex dust lanes scattered throughout the optically recognizable bands of the Milky Way. These bands become bright at (sub-)millimetre wavelengths, where we can study dust thermal emission and the chemical and kinematic signatures of the gas. To properly study such large-scale environments, requires deep, large area surveys that are not possible with current facilities. Moreover, where stars form, so too do planetary systems, growing from the dust and gas in circumstellar discs, to planets and planetesimal belts. Understanding the evolution of these belts requires deep imaging capable of studying belts around young stellar objects to Kuiper belt analogues around the nearest stars. Here we present a plan for observing the Galactic Plane and circumstellar environments to quantify the physical structure, the magnetic fields, the dynamics, chemistry, star formation, and planetary system evolution of the galaxy in which we live with AtLAST; a concept for a new, 50m single-dish sub-mm telescope with a large field of view which is the only type of facility that will allow us to observe our Galaxy deeply and widely enough to make a leap forward in our understanding of our local ecology.

随着我们对银河系多尺度星际介质(ISM)了解的增多,我们对巨分子云(GMC)中大尺度弥漫气体和尘埃之间的复杂关系、它们如何运动、如何受到附近大质量恒星的影响以及这些巨分子云中的哪些部分最终坍缩为恒星形成区有了更深入的了解。这些气体、尘埃和恒星群之间复杂的相互作用形成了我们银河系的生态。由于我们深深地嵌入了银河系的平面,它占据了天空的很大一部分,复杂的尘埃通道散布在整个银河系的光学可识别带中。这些波段在(亚)毫米波长下变得明亮,我们可以在这里研究尘埃的热辐射以及气体的化学和运动特征。要正确研究这样的大尺度环境,需要进行深层次、大面积的巡天观测,而目前的设备无法做到这一点。此外,恒星形成的地方也会形成行星系统,从星盘周围的尘埃和气体发展到行星和行星带。要了解这些星带的演变过程,就需要对年轻恒星天体周围的星带以及最近恒星周围的柯伊伯带类似物进行深度成像研究。在这里,我们提出了一个利用 AtLAST 观测银河系平面和星环环境的计划,以量化我们所生活的银河系的物理结构、磁场、动力学、化学、恒星形成和行星系统演化;这是一个新的 50 米单碟亚微米大视场望远镜的概念,它是唯一一种能够让我们深入而广泛地观测银河系的设施,足以使我们对本地生态的认识向前迈进一大步。
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引用次数: 0
Developing Inclusive Community Platforms: A Catalyst for DEI Community 开发包容性社区平台:促进社区 DEI
Pub Date : 2024-06-05 DOI: 10.12688/openreseurope.17653.1
Hiroko Costantini, Misato Nihei, Takazumi Ono, Kai Tanabe, Yoichi Motomura
Developing inclusive communities is important to enhance individuals’ well-being yet this brings the challenge of actively engaging and leveraging the diversity of residents in communities. Such significant social challenges are prominent in Japan, a focus of this article, as the most advanced aging society in the world and thus relevant to European and other countries. This paper explains a major government initiative that takes an innovative approach through leveraging a social technology, a Digital Twin of a community, to understand and address inclusiveness of a community leveraging population approach. The purpose of this letter is to provide researchers and policy makers insights into the approach taken to stimulate reflection on the potential for adaptation and replication.
发展包容性社区对提高个人福祉非常重要,但这也带来了如何积极吸引和利用社区居民多样性的挑战。日本是世界上最发达的老龄化社会,因此对欧洲和其他国家也有借鉴意义。本文介绍了政府的一项重大举措,该举措通过利用社会技术--社区数字孪生图--来了解和解决社区利用人口的包容性问题。这封信的目的是让研究人员和政策制定者深入了解所采取的方法,以激发他们对调整和复制潜力的思考。
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引用次数: 0
Memorializing Women on the Move: A register of migrant women landmarks in Europe 纪念迁徙中的妇女:欧洲移民妇女地标登记册
Pub Date : 2024-06-04 DOI: 10.12688/openreseurope.17052.1
Maija Ojala-Fulwood, Bénédicte Miyamoto, Marie Ruiz, Heidi Martins, F. Bakas, Veronika Čapská, Frigren Pirita, Lívia Prosinger, Igor Lyman, Victoria Konstantinova
This dataset was developed by COST Action 19112 Women on the Move (WEMov), which engages in unveiling women migrants' presence and participation in the construction of Europe. The dataset was built as a register of toponyms and monuments in the political and public landscape in Europe – such as street names, school names and parks, as well as statues and memorials – that celebrate women migrants. With the dataset we want to discover how women migrants are remembered and what kind of landmarks present these individuals who have had an episode of migration for a variety of reasons. Moreover, our aim is to make these landmarks and the stories of women migrants visible by presenting the results of the dataset in an interactive map on our website. At the moment, the dataset includes 1000 landmarks. The collection of data was based on voluntary work of scholars and students from over 40 different European countries. We have aimed for broad geographical coverage; however, some areas are better represented than others due the nature of data collection. The collection of data is an ongoing process and therefore the dataset in Nakala repository, to which this data note refers, presents the situation in July 2023. Updated versions of the dataset will be made available in Nakala and we will download new landmarks to our interactive map on a regular basis. The selected landmarks and migrant trajectories feature cross-community or cross-cultural migration. They show both typical and exceptional forms of mobility and present women of different age, profession, social status and migration status. This intersectionality of the project and the dataset highlights not only the richness of these landmarks and their value for scholarship but also the wide spectrum of migrant women and their contribution to society.
该数据集由 COST 行动 19112 "移动中的妇女"(WEMov)开发,旨在揭示女性移民在欧洲建设中的存在和参与。该数据集是作为欧洲政治和公共景观中纪念女性移民的地名和纪念碑(如街道名称、学校名称和公园,以及雕像和纪念碑)的登记册而建立的。通过该数据集,我们希望了解人们是如何记住女性移民的,以及哪些地标展示了这些因各种原因而经历过移民的人。此外,我们的目标是通过网站上的互动地图展示数据集的结果,让人们了解这些地标和女性移民的故事。目前,数据集包括 1000 个地标。数据的收集基于来自 40 多个不同欧洲国家的学者和学生的志愿工作。我们的目标是实现广泛的地理覆盖;不过,由于数据收集的性质,有些地区的代表性要好于其他地区。数据收集是一个持续的过程,因此,本数据说明所提及的 Nakala 储存库中的数据集介绍的是 2023 年 7 月的情况。数据集的更新版本将在 Nakala 中提供,我们将定期向互动地图下载新的地标。所选地标和移民轨迹以跨社区或跨文化移民为特征。它们展示了典型和特殊的流动形式,呈现了不同年龄、职业、社会地位和移民身份的妇女。该项目和数据集的这种交叉性不仅凸显了这些地标的丰富性及其学术价值,而且也凸显了移民妇女的广泛性及其对社会的贡献。
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引用次数: 0
Therapeutic relationship elements and therapy session outcomes: Protocol for a longitudinal study of the patient's perspective. 治疗关系要素与治疗疗程结果:病人视角纵向研究协议。
Pub Date : 2024-06-04 eCollection Date: 2023-01-01 DOI: 10.12688/openreseurope.16466.2
Alberto Stefana, Paolo Fusar-Poli, Eduard Vieta, Eric A Youngstrom

Background: There is a growing recognition of the key role of the therapeutic relationship in the outcomes of psychotherapy. However, current understanding of its specific components, their interplay and related patient-therapist dynamics is limited.

Objective: (a) To validate two self-report measures to assess subjective affective reactions of patients toward their psychotherapists during specific therapy sessions, and (b) to explore the relationships and dynamics among four elements of the therapeutic relationship: patient reactions toward the therapist, working alliance, alliance ruptures and repairs, and the real relationship.

Methods: This study uses a nonrandomized, two-time point longitudinal design. The target population is adult patients currently engaged in individual psychotherapy for heterogeneous mental conditions. Participants are recruited through two online recruitment platforms: Research for Me and ResearchMatch. Data collection involves administering two surveys through the Qualtrics online survey platform. The baseline survey assesses information about the most recent therapy session and the preceding week, while the follow-up survey collects data on the subsequent therapy session and the days leading up to it.

Discussion: This research offers three main contributions: (a) it furthers evidence-based assessment in psychotherapy by creating and validating two novel, succinct self-report tools; (b) it enhances theoretical understanding within therapeutic relationship research by exploring the significant impact of patients' perceptions of relationship elements on session outcomes variability; and (c) it will identify therapeutic relationship elements that can either enhance or hinder the overall relationship quality and session outcomes.

Ethics and dissemination: The study protocol was approved by the Institutional Review Board of the University of North Carolina at Chapel Hill. The results will be published in indexed peer-reviewed journals and presented at relevant psychology and psychiatry conferences.

背景:越来越多的人认识到治疗关系在心理治疗结果中的关键作用。目的:(a)验证两种自我报告测量方法,以评估患者在特定治疗过程中对心理治疗师的主观情感反应;(b)探索治疗关系四个要素之间的关系和动态变化:患者对治疗师的反应、工作联盟、联盟破裂和修复以及真实关系:本研究采用非随机、双时间点纵向设计。研究对象是目前正在接受个体心理治疗的成年患者,他们的精神状况各不相同。参与者通过两个在线招募平台招募:Research for Me 和 ResearchMatch。数据收集包括通过 Qualtrics 在线调查平台进行两项调查。基线调查评估最近一次治疗及之前一周的信息,而后续调查则收集随后一次治疗及之前几天的数据:本研究有三大贡献:(a)通过创建和验证两种新颖、简洁的自我报告工具,促进了心理治疗中的循证评估;(b)通过探索患者对治疗关系要素的认知对疗程结果可变性的重要影响,加强了治疗关系研究中的理论理解;(c)将确定可提高或阻碍整体关系质量和疗程结果的治疗关系要素:研究方案已获得北卡罗来纳大学教堂山分校机构审查委员会的批准。研究结果将发表在有索引的同行评审期刊上,并在相关的心理学和精神病学会议上发表。
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引用次数: 0
Lockbox enrichment facilitates manipulative and cognitive activities for mice 丰富锁箱有利于小鼠的操作和认知活动
Pub Date : 2024-06-04 DOI: 10.12688/openreseurope.17624.1
K. Hohlbaum, Niek Andresen, P. Mieske, P. Kahnau, Benjamin Lang, K. Diederich, Rupert Palme, Lars Mundhenk, Henning Sprekeler, Olaf Hellwich, Christa Thöne-Reineke, L. Lewejohann
Background Due to the lack of complexity and variety of stimuli, conventional housing conditions of laboratory mice do not allow these animals to fully express their behavioral repertoire, including manipulative and cognitive activities. Therefore, we designed mechanical puzzles, so-called lockboxes, for mice that can be provided in their home cages. We investigated the impact of the lockbox enrichment on their phenotype and affective state when compared to conventional housing and super-environmental enrichment. Methods Young adult female C57BL/6JCrl mice were examined before and after 2-month exposure to the different types of enrichment in a phenotyping test battery, including tests for trait and state anxiety-related behavior, calorimetric measurements, body weight measurements, and the analysis of stress hormone metabolite concentrations as well as sequential problem-solving abilities. At the end of the study, adrenal gland weights were determined and pathohistological evaluation was performed. For all continuous variables, the relative variability was calculated. Results We demonstrated that lockbox enrichment decreased trait anxiety-related behavior compared to conventional housing in the Grid Exploratory Paradigm. In contrast, the different types of enrichment neither influenced state anxiety-related behavior in the Open Field and Elevated Plus Maze Test nor physiological variables (i.e., bodyweight, resting metabolic rate, stress hormone metabolite concentrations, and adrenal gland weights). Lockbox enrichment improved sequential problem-solving when compared to super-environmental enrichment. Regardless of the housing condition, the relative variability increased in most variables over time, although the coefficient of variation decreased for some variables, especially in animals with access to the lockbox enrichment. A pathohistological evaluation revealed no evidence of toxicopathological effects associated with the material from which the lockbox enrichment was made. Conclusions Overall, the lockbox enrichment revealed beneficial effects on the affective state and sequential problem-solving abilities of laboratory mice. The reproducibility was demonstrated not to be compromised by lockbox enrichment, similar to super-environmental enrichment.
背景 由于缺乏复杂多样的刺激,实验室小鼠的传统饲养条件无法让这些动物充分表达其行为,包括操作和认知活动。因此,我们为小鼠设计了机械拼图,即所谓的 "锁盒",可以放在小鼠的家笼里。与传统饲养方式和超级环境富集相比,我们研究了锁盒富集对小鼠表型和情感状态的影响。方法 对年轻的成年雌性 C57BL/6JCrl 小鼠进行为期 2 个月的表型测试,包括性状和状态焦虑相关行为测试、热量测量、体重测量、应激激素代谢物浓度分析以及连续问题解决能力分析。研究结束时,测定肾上腺重量并进行病理组织学评估。计算了所有连续变量的相对变异性。结果 我们证明,在网格探索范式中,与传统饲养方式相比,锁箱强化训练可减少与焦虑相关的行为。与此相反,不同类型的强化训练既不会影响开阔地和高架迷宫测试中与状态焦虑相关的行为,也不会影响生理变量(即体重、静息代谢率、应激激素代谢物浓度和肾上腺重量)。与超级环境富集相比,锁定箱富集提高了顺序问题解决能力。无论饲养条件如何,大多数变量的相对变异性都会随着时间的推移而增加,但某些变量的变异系数会降低,特别是在可以使用锁箱强化饲养的动物中。病理组织学评估结果显示,没有证据表明锁箱增容材料会产生毒理病理学影响。结论 总体而言,富集锁盒对实验鼠的情绪状态和连续解决问题的能力有好处。与超级环境富集类似,锁盒富集不会影响重现性。
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