Pub Date : 2024-09-16eCollection Date: 2023-01-01DOI: 10.12688/openreseurope.15122.2
Ricardo Rebelo, Luis Fialho, Maria Helena Novais
Background: Floating photovoltaics (FPV) is an emerging technology that is gaining attention worldwide. However, little information is still available on its possible impacts in the aquatic ecosystems, as well as on the durability of its components. Therefore, this work intends to provide a contribution to this field, analysing possible obstacles that can compromise the performance of this technology, adding to an increase of its reliability and assessing possible impacts.The problem under study is related to the potential submersion of photovoltaic cables, that can lead to a degradation of its electrical insulation capabilities and, consequently, higher energy production losses and water contamination.
Methods: In the present study, the submersion of photovoltaic cables (with two different insulation materials) in freshwater and artificial seawater was tested, in order to replicate real life conditions, when FPV systems are located in reservoirs or in the marine environment. Electrical insulation tests were carried out weekly to assess possible cable degradation, the physical-chemical characteristics of the water were also periodically monitored, complemented by analysis to detect traces of copper and microplastics in the water.
Results: The results showed that the submersion of photovoltaic cables with rubber sheath in saltwater can lead to a cable accelerated degradation, with reduction of its electrical insulation and, consequently, copper release into the aquatic environment.
Conclusions: The test results pointed a probable relationship between submersion of cables with rubber outer shell and water freezing temperatures and the occurrence of accelerated degradation of the cable insulation layer. Reduced insulation resistance values were measured in this cable type after the occurrence of such temperatures, both in salt and freshwater, the cable presented visible exterior degradation signs. For this case copper residues were detected in the water.
{"title":"Floating photovoltaic systems: photovoltaic cable submersion testing and potential impacts.","authors":"Ricardo Rebelo, Luis Fialho, Maria Helena Novais","doi":"10.12688/openreseurope.15122.2","DOIUrl":"https://doi.org/10.12688/openreseurope.15122.2","url":null,"abstract":"<p><strong>Background: </strong>Floating photovoltaics (FPV) is an emerging technology that is gaining attention worldwide. However, little information is still available on its possible impacts in the aquatic ecosystems, as well as on the durability of its components. Therefore, this work intends to provide a contribution to this field, analysing possible obstacles that can compromise the performance of this technology, adding to an increase of its reliability and assessing possible impacts.The problem under study is related to the potential submersion of photovoltaic cables, that can lead to a degradation of its electrical insulation capabilities and, consequently, higher energy production losses and water contamination.</p><p><strong>Methods: </strong>In the present study, the submersion of photovoltaic cables (with two different insulation materials) in freshwater and artificial seawater was tested, in order to replicate real life conditions, when FPV systems are located in reservoirs or in the marine environment. Electrical insulation tests were carried out weekly to assess possible cable degradation, the physical-chemical characteristics of the water were also periodically monitored, complemented by analysis to detect traces of copper and microplastics in the water.</p><p><strong>Results: </strong>The results showed that the submersion of photovoltaic cables with rubber sheath in saltwater can lead to a cable accelerated degradation, with reduction of its electrical insulation and, consequently, copper release into the aquatic environment.</p><p><strong>Conclusions: </strong>The test results pointed a probable relationship between submersion of cables with rubber outer shell and water freezing temperatures and the occurrence of accelerated degradation of the cable insulation layer. Reduced insulation resistance values were measured in this cable type after the occurrence of such temperatures, both in salt and freshwater, the cable presented visible exterior degradation signs. For this case copper residues were detected in the water.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"3 ","pages":"61"},"PeriodicalIF":0.0,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142395786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-09eCollection Date: 2024-01-01DOI: 10.12688/openreseurope.17000.2
Mariana Carreira, Manuel Pires-Santos, Clara R Correia, Sara Nadine, João F Mano
Background: Surface topography has been shown to influence cell behavior and direct stromal cell differentiation into distinct lineages. Whereas this phenomenon has been verified in two-dimensional cultures, there is an urgent need for a thorough investigation of topography's role within a three-dimensional (3D) environment, as it better replicates the natural cellular environment.
Methods: A co-culture of Wharton's jelly-derived mesenchymal stem/stromal cells (WJ-MSCs) and human umbilical vein endothelial cells (HUVECs) was encapsulated in a 3D system consisting of a permselective liquefied environment containing freely dispersed spherical microparticles (spheres) or nanogrooved microdiscs (microdiscs). Microdiscs presenting 358 ± 23 nm grooves and 944 ± 49 nm ridges were produced via nanoimprinting of spherical polycaprolactone microparticles between water-soluble polyvinyl alcohol counter molds of nanogrooved templates. Spheres and microdiscs were cultured in vitro with umbilical cord-derived cells in a basal or osteogenic medium within liquefied capsules for 21 days.
Results: WJ-MSCs and HUVECs were successfully encapsulated within liquefied capsules containing spheres and microdiscs, ensuring high cellular viability. Results show an enhanced osteogenic differentiation in microdiscs compared to spheres, even in basal medium, evidenced by alkaline phosphatase activity and osteopontin expression.
Conclusions: This work suggests that the topographical features present in microdiscs induce the osteogenic differentiation of adhered WJ-MSCs along the contact guidance, without additional differentiation factors. The developed 3D bioencapsulation system comprising topographical features might be suitable for bone tissue engineering approaches with minimum in vitro manipulation.
{"title":"Liquefied capsules containing nanogrooved microdiscs and umbilical cord-derived cells for bone tissue engineering.","authors":"Mariana Carreira, Manuel Pires-Santos, Clara R Correia, Sara Nadine, João F Mano","doi":"10.12688/openreseurope.17000.2","DOIUrl":"10.12688/openreseurope.17000.2","url":null,"abstract":"<p><strong>Background: </strong>Surface topography has been shown to influence cell behavior and direct stromal cell differentiation into distinct lineages. Whereas this phenomenon has been verified in two-dimensional cultures, there is an urgent need for a thorough investigation of topography's role within a three-dimensional (3D) environment, as it better replicates the natural cellular environment.</p><p><strong>Methods: </strong>A co-culture of Wharton's jelly-derived mesenchymal stem/stromal cells (WJ-MSCs) and human umbilical vein endothelial cells (HUVECs) was encapsulated in a 3D system consisting of a permselective liquefied environment containing freely dispersed spherical microparticles (spheres) or nanogrooved microdiscs (microdiscs). Microdiscs presenting 358 ± 23 nm grooves and 944 ± 49 nm ridges were produced via nanoimprinting of spherical polycaprolactone microparticles between water-soluble polyvinyl alcohol counter molds of nanogrooved templates. Spheres and microdiscs were cultured <i>in vitro</i> with umbilical cord-derived cells in a basal or osteogenic medium within liquefied capsules for 21 days.</p><p><strong>Results: </strong>WJ-MSCs and HUVECs were successfully encapsulated within liquefied capsules containing spheres and microdiscs, ensuring high cellular viability. Results show an enhanced osteogenic differentiation in microdiscs compared to spheres, even in basal medium, evidenced by alkaline phosphatase activity and osteopontin expression.</p><p><strong>Conclusions: </strong>This work suggests that the topographical features present in microdiscs induce the osteogenic differentiation of adhered WJ-MSCs along the contact guidance, without additional differentiation factors. The developed 3D bioencapsulation system comprising topographical features might be suitable for bone tissue engineering approaches with minimum <i>in vitro</i> manipulation.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"4 ","pages":"94"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11393531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05eCollection Date: 2023-01-01DOI: 10.12688/openreseurope.16535.3
Padraic Rocliffe, Ian Sherwin, Patricia Mannix-McNamara, Ciaran MacDonncha, Brendan T O' Keeffe
Background: The aim of this study was to examine the test-retest reliability of the physical activity behavior, health and wellbeing questionnaire, in adolescent populations, administered by teachers in school settings, in the Republic of Ireland.
Methods: A cross-sectional, mixed sample of 55 participants (45.5% males: Age, 13.94 (±.40) years) were included. The participants completed the questionnaire on two occasions (T1 and T2), on the same day and time, one week apart following identical procedures. Variables for testing included physical activity behavior (n=13), health (n=11) and wellbeing (n=2). Test-retest reliability of the questionnaire's covariates, including family affluence and physical impairments were also examined.
Results: Systematic error (Bland-Altman plots) was found to be near to zero for each of the physical activity behavior, health and wellbeing variables. The combined mean coefficient of variation was lower for females (10.19%) in comparison to males (13.01%). The combined mean intraclass correlation coefficients were higher for females (0.901) than males (0.822). Similarly, the combined mean Cronbach alpha coefficient were higher for girls (0.908) than boys (0.821).
Conclusions: This study found the physical activity behavior, health and wellbeing questionnaire to be reliable for use in adolescent populations.
{"title":"Test-Retest Reliability of a Physical Activity Behavior, Health and Wellbeing Questionnaire in Adolescents.","authors":"Padraic Rocliffe, Ian Sherwin, Patricia Mannix-McNamara, Ciaran MacDonncha, Brendan T O' Keeffe","doi":"10.12688/openreseurope.16535.3","DOIUrl":"10.12688/openreseurope.16535.3","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to examine the test-retest reliability of the physical activity behavior, health and wellbeing questionnaire, in adolescent populations, administered by teachers in school settings, in the Republic of Ireland.</p><p><strong>Methods: </strong>A cross-sectional, mixed sample of 55 participants (45.5% males: Age, 13.94 (±.40) years) were included. The participants completed the questionnaire on two occasions (T1 and T2), on the same day and time, one week apart following identical procedures. Variables for testing included physical activity behavior (n=13), health (n=11) and wellbeing (n=2). Test-retest reliability of the questionnaire's covariates, including family affluence and physical impairments were also examined.</p><p><strong>Results: </strong>Systematic error (Bland-Altman plots) was found to be near to zero for each of the physical activity behavior, health and wellbeing variables. The combined mean coefficient of variation was lower for females (10.19%) in comparison to males (13.01%). The combined mean intraclass correlation coefficients were higher for females (0.901) than males (0.822). Similarly, the combined mean Cronbach alpha coefficient were higher for girls (0.908) than boys (0.821).</p><p><strong>Conclusions: </strong>This study found the physical activity behavior, health and wellbeing questionnaire to be reliable for use in adolescent populations.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"3 ","pages":"154"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142156818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-03eCollection Date: 2024-01-01DOI: 10.12688/openreseurope.17421.1
Stefania Mainieri
Faces Revealed Project established a new methodology for studying the geometry of the human forms and facial features realized on anthropoid yellow coffins of Ancient Egypt. Since 1980, yellow coffins have been the subject of various studies mainly focused on iconography and palaeography. However, these anthropoid coffins are three-dimensional objects with well-rendered masks and detailed facial features as well as forearms, hands and bellies. This lack of analysis in the study of coffins may be due to the fact that they are "concealed" by rich and multi-coloured decoration, so they are not easily visible in all their forms to the naked eye. Today new technologies allow us to go more in-depth and digitally switch off the decoration and observe these "invisible" features. As this is an entirely new process, the primary task of the Faces Revealed Project was to establish a new methodology from the photogrammetric survey to the data collection. The present article discusses in detail the stages of the Project applied to around 100 Egyptian yellow coffins stored in Museums in Europe, the United States and Egypt and the information that they can disclose. The task is to share with the scientific community the established protocol and offering the possibility to "work independently" applying the same methodology to the same objects as well as to other classes of material.
{"title":"Faces Revealed Project and ancient Egyptian yellow coffins: A new methodology step-by-step.","authors":"Stefania Mainieri","doi":"10.12688/openreseurope.17421.1","DOIUrl":"https://doi.org/10.12688/openreseurope.17421.1","url":null,"abstract":"<p><p>Faces Revealed Project established a new methodology for studying the geometry of the human forms and facial features realized on anthropoid yellow coffins of Ancient Egypt. Since 1980, yellow coffins have been the subject of various studies mainly focused on iconography and palaeography. However, these anthropoid coffins are three-dimensional objects with well-rendered masks and detailed facial features as well as forearms, hands and bellies. This lack of analysis in the study of coffins may be due to the fact that they are \"concealed\" by rich and multi-coloured decoration, so they are not easily visible in all their forms to the naked eye. Today new technologies allow us to go more in-depth and digitally switch off the decoration and observe these \"invisible\" features. As this is an entirely new process, the primary task of the Faces Revealed Project was to establish a new methodology from the photogrammetric survey to the data collection. The present article discusses in detail the stages of the Project applied to around 100 Egyptian yellow coffins stored in Museums in Europe, the United States and Egypt and the information that they can disclose. The task is to share with the scientific community the established protocol and offering the possibility to \"work independently\" applying the same methodology to the same objects as well as to other classes of material.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"4 ","pages":"193"},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11464963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-02eCollection Date: 2023-01-01DOI: 10.12688/openreseurope.16693.2
Md Nasimul Islam Maruf, Shadman Mahmud, Iván S Pasarín, Federico Giani, Aurélien Degrave, Carlos Funez Guerra, Susana Lopez, Ivan Mesonero
Background: Energy communities facilitate several advantages, including energy autonomy, reduced greenhouse gas emissions, poverty mitigation, and regional economic development. They also empower citizens with decision-making and co-ownership prospects in community renewable projects. Integrating renewable energy sources and sector coupling is a crucial strategy for flexible energy systems. However, demonstrating clean energy transition scenarios in these communities presents challenges, including technology integration, flexibility activation, load reduction, grid resilience, and business case development.
Methods: Based on the system of systems approach, this paper introduces a 4-step funnel approach and a 4-step reverse funnel approach to systematically specify and detail demonstration scenarios for energy community projects. The funnel approach involves four steps. First, it selects demonstration scenarios promoting energy-efficient state-of-the-art renewable technologies and storage systems, flexibility through demand side management techniques, reduced grid dependence, and economic viability. Second, it lists all existing and planned project technologies, analysing energy flows. Third, it plans actions at different levels to implement the demonstration scenarios. Fourth, it validates the strategies using key performance indicators (KPI) to quantify the effectiveness of the planned measures. Furthermore, the reverse funnel approach delves deeper into the demonstration scenarios. The four steps involve identifying stakeholder perspectives, describing scenario scopes, listing conditions for realisation, and outlining business models, including value chains and economic assumptions.
Results: This approach provides a detailed analysis of the demonstration scenarios, considering actors, objectives, boundary conditions, and business assumptions. The methodologies are exemplified in three diverse European energy communities extending across residential, commercial, tertiary, and industrial establishments, allowing power-to-x and sector coupling opportunities. The paper also suggested thirteen KPIs for validating renewable-focused energy community projects.
Conclusions: Finally, the paper recommends increased collaboration between energy communities, knowledge sharing, stakeholder engagement, transparent data collection and analysis, continuous feedback, and method improvement to mitigate policy, technology, business, and market uncertainties.
{"title":"Demonstrating clean energy transition scenarios in sector-coupled and renewable-based energy communities.","authors":"Md Nasimul Islam Maruf, Shadman Mahmud, Iván S Pasarín, Federico Giani, Aurélien Degrave, Carlos Funez Guerra, Susana Lopez, Ivan Mesonero","doi":"10.12688/openreseurope.16693.2","DOIUrl":"https://doi.org/10.12688/openreseurope.16693.2","url":null,"abstract":"<p><strong>Background: </strong>Energy communities facilitate several advantages, including energy autonomy, reduced greenhouse gas emissions, poverty mitigation, and regional economic development. They also empower citizens with decision-making and co-ownership prospects in community renewable projects. Integrating renewable energy sources and sector coupling is a crucial strategy for flexible energy systems. However, demonstrating clean energy transition scenarios in these communities presents challenges, including technology integration, flexibility activation, load reduction, grid resilience, and business case development.</p><p><strong>Methods: </strong>Based on the system of systems approach, this paper introduces a 4-step funnel approach and a 4-step reverse funnel approach to systematically specify and detail demonstration scenarios for energy community projects. The funnel approach involves four steps. First, it selects demonstration scenarios promoting energy-efficient state-of-the-art renewable technologies and storage systems, flexibility through demand side management techniques, reduced grid dependence, and economic viability. Second, it lists all existing and planned project technologies, analysing energy flows. Third, it plans actions at different levels to implement the demonstration scenarios. Fourth, it validates the strategies using key performance indicators (KPI) to quantify the effectiveness of the planned measures. Furthermore, the reverse funnel approach delves deeper into the demonstration scenarios. The four steps involve identifying stakeholder perspectives, describing scenario scopes, listing conditions for realisation, and outlining business models, including value chains and economic assumptions.</p><p><strong>Results: </strong>This approach provides a detailed analysis of the demonstration scenarios, considering actors, objectives, boundary conditions, and business assumptions. The methodologies are exemplified in three diverse European energy communities extending across residential, commercial, tertiary, and industrial establishments, allowing power-to-x and sector coupling opportunities. The paper also suggested thirteen KPIs for validating renewable-focused energy community projects.</p><p><strong>Conclusions: </strong>Finally, the paper recommends increased collaboration between energy communities, knowledge sharing, stakeholder engagement, transparent data collection and analysis, continuous feedback, and method improvement to mitigate policy, technology, business, and market uncertainties.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"3 ","pages":"193"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-28eCollection Date: 2024-01-01DOI: 10.12688/openreseurope.17624.2
Katharina Hohlbaum, Niek Andresen, Paul Mieske, Pia Kahnau, Benjamin Lang, Kai Diederich, Rupert Palme, Lars Mundhenk, Henning Sprekeler, Olaf Hellwich, Christa Thöne-Reineke, Lars Lewejohann
Background: Due to the lack of complexity and variety of stimuli, conventional housing conditions of laboratory mice do not allow these animals to fully express their behavioral repertoire, including manipulative and cognitive activities. Therefore, we designed mechanical puzzles, so-called lockboxes, for mice that can be provided in their home cages. We investigated the impact of the lockbox enrichment on their phenotype and affective state when compared to conventional housing (CH) and super-environmental enrichment (SEE).
Methods: Young adult female C57BL/6JCrl mice were examined before and after 2-month exposure to the different types of enrichment in a phenotyping test battery, including tests for trait and state anxiety-related behavior, calorimetric measurements, body weight measurements, the analysis of stress hormone metabolite concentrations, and sequential problem-solving abilities with a novel lockbox. At the end of the study, adrenal gland weights were determined and pathohistological evaluation was performed. For all continuous variables, the relative variability was calculated.
Results: While the different types of enrichment affected trait anxiety-related behavior, neither state anxiety-related behavior nor physiological variables (i.e., bodyweight, resting metabolic rate, stress hormone metabolite concentrations, adrenal gland weights) were influenced. LE improved sequential problem-solving (i.e., solving novel lockboxes) when compared to SEE. Regardless of the housing condition, the relative variability increased in most variables over time, although the coefficient of variation decreased for some variables, especially in animals with access to LE. There was no evidence of toxicopathological effects associated with the material from which the lockboxes were made.
Conclusions: All lockboxes are available as open-source tool. LE revealed beneficial effects on the affective state of laboratory mice and their performance in solving novel lockboxes. Neither relevant phenotype of the mice nor reproducibility of the data were compromised by LE, similar to SEE. The lockboxes may also be used as novel approach for assessing cognition in mice.
背景:由于缺乏复杂多样的刺激,实验室小鼠的传统饲养条件无法让这些动物充分表达其行为,包括操作和认知活动。因此,我们为小鼠设计了机械拼图,即所谓的 "锁盒",可以放在小鼠的家笼里。与传统饲养(CH)和超级环境强化(SEE)相比,我们研究了锁盒强化对小鼠表型和情感状态的影响:年轻的成年雌性C57BL/6JCrl小鼠在暴露于不同类型的富集环境2个月前后接受了一系列表型测试,包括性状和状态焦虑相关行为测试、热量测量、体重测量、应激激素代谢物浓度分析以及使用新型锁箱连续解决问题的能力。研究结束时,测定了肾上腺重量,并进行了病理组织学评估。对所有连续变量都计算了相对变异性:结果:虽然不同类型的强化训练会影响特质焦虑相关行为,但不会影响状态焦虑相关行为或生理变量(即体重、静息代谢率、应激激素代谢物浓度、肾上腺重量)。与 SEE 相比,LE 提高了连续问题解决能力(即解决新锁箱问题)。无论饲养条件如何,大多数变量的相对变异性都会随着时间的推移而增加,但某些变量的变异系数会降低,特别是在有机会进入LE的动物中。没有证据表明毒理学效应与制造锁箱的材料有关:所有锁盒都是开源工具。LE对实验鼠的情绪状态和解决新锁盒问题的表现都有益处。小鼠的相关表型和数据的可重复性都没有受到 LE 的影响,这一点与 SEE 相似。锁箱也可作为评估小鼠认知能力的新方法。
{"title":"Lockbox enrichment facilitates manipulative and cognitive activities for mice.","authors":"Katharina Hohlbaum, Niek Andresen, Paul Mieske, Pia Kahnau, Benjamin Lang, Kai Diederich, Rupert Palme, Lars Mundhenk, Henning Sprekeler, Olaf Hellwich, Christa Thöne-Reineke, Lars Lewejohann","doi":"10.12688/openreseurope.17624.2","DOIUrl":"https://doi.org/10.12688/openreseurope.17624.2","url":null,"abstract":"<p><strong>Background: </strong>Due to the lack of complexity and variety of stimuli, conventional housing conditions of laboratory mice do not allow these animals to fully express their behavioral repertoire, including manipulative and cognitive activities. Therefore, we designed mechanical puzzles, so-called lockboxes, for mice that can be provided in their home cages. We investigated the impact of the lockbox enrichment on their phenotype and affective state when compared to conventional housing (CH) and super-environmental enrichment (SEE).</p><p><strong>Methods: </strong>Young adult female C57BL/6JCrl mice were examined before and after 2-month exposure to the different types of enrichment in a phenotyping test battery, including tests for trait and state anxiety-related behavior, calorimetric measurements, body weight measurements, the analysis of stress hormone metabolite concentrations, and sequential problem-solving abilities with a novel lockbox. At the end of the study, adrenal gland weights were determined and pathohistological evaluation was performed. For all continuous variables, the relative variability was calculated.</p><p><strong>Results: </strong>While the different types of enrichment affected trait anxiety-related behavior, neither state anxiety-related behavior nor physiological variables (i.e., bodyweight, resting metabolic rate, stress hormone metabolite concentrations, adrenal gland weights) were influenced. LE improved sequential problem-solving (i.e., solving novel lockboxes) when compared to SEE. Regardless of the housing condition, the relative variability increased in most variables over time, although the coefficient of variation decreased for some variables, especially in animals with access to LE. There was no evidence of toxicopathological effects associated with the material from which the lockboxes were made.</p><p><strong>Conclusions: </strong>All lockboxes are available as open-source tool. LE revealed beneficial effects on the affective state of laboratory mice and their performance in solving novel lockboxes. Neither relevant phenotype of the mice nor reproducibility of the data were compromised by LE, similar to SEE. The lockboxes may also be used as novel approach for assessing cognition in mice.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"4 ","pages":"108"},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11384198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-28eCollection Date: 2024-01-01DOI: 10.12688/openreseurope.17023.2
Seán Enis Cody, Sebastian Scher, Iain McDonald, Albert Zijlstra, Emma Alexander, Nick Cox
Background: Identifying stars belonging to different classes is vital in order to build up statistical samples of different phases and pathways of stellar evolution. In the era of surveys covering billions of stars, an automated method of identifying these classes becomes necessary.
Methods: Many classes of stars are identified based on their emitted spectra. In this paper, we use a combination of the multi-class multi-label Machine Learning (ML) method XGBoost and the PySSED spectral-energy-distribution fitting algorithm to classify stars into nine different classes, based on their photometric data. The classifier is trained on subsets of the SIMBAD database. Particular challenges are the very high sparsity (large fraction of missing values) of the underlying data as well as the high class imbalance. We discuss the different variables available, such as photometric measurements on the one hand, and indirect predictors such as Galactic position on the other hand.
Results: We show the difference in performance when excluding certain variables, and discuss in which contexts which of the variables should be used. Finally, we show that increasing the number of samples of a particular type of star significantly increases the performance of the model for that particular type, while having little to no impact on other types. The accuracy of the main classifier is ∼0.7 with a macro F1 score of 0.61.
Conclusions: While the current accuracy of the classifier is not high enough to be reliably used in stellar classification, this work is an initial proof of feasibility for using ML to classify stars based on photometry.
背景:要建立恒星演化不同阶段和途径的统计样本,识别属于不同类别的恒星至关重要。在对数十亿恒星进行巡天观测的时代,有必要采用一种自动方法来识别这些类别:方法:许多恒星类别都是根据它们的发射光谱来识别的。在本文中,我们结合使用多类多标签机器学习(ML)方法 XGBoost 和 PySSED 光谱能量分布拟合算法,根据测光数据将恒星分为九个不同的类别。分类器是在 SIMBAD 数据库的子集上进行训练的。所面临的特殊挑战是基础数据的高度稀疏性(大量缺失值)以及高度的类别不平衡。我们讨论了可用的不同变量,一方面是光度测量数据,另一方面是银河系位置等间接预测指标:结果:我们展示了排除某些变量后的性能差异,并讨论了在哪些情况下应使用哪些变量。最后,我们展示了增加特定类型恒星的样本数量会显著提高模型对该特定类型恒星的性能,而对其他类型恒星几乎没有影响。主分类器的准确率为 0.7,宏观 F1 得分为 0.61.结论:虽然目前分类器的准确率还不够高,不能可靠地用于恒星分类,但这项工作初步证明了使用 ML 根据光度测量对恒星进行分类的可行性。
{"title":"Machine learning based stellar classification with highly sparse photometry data.","authors":"Seán Enis Cody, Sebastian Scher, Iain McDonald, Albert Zijlstra, Emma Alexander, Nick Cox","doi":"10.12688/openreseurope.17023.2","DOIUrl":"https://doi.org/10.12688/openreseurope.17023.2","url":null,"abstract":"<p><strong>Background: </strong>Identifying stars belonging to different classes is vital in order to build up statistical samples of different phases and pathways of stellar evolution. In the era of surveys covering billions of stars, an automated method of identifying these classes becomes necessary.</p><p><strong>Methods: </strong>Many classes of stars are identified based on their emitted spectra. In this paper, we use a combination of the multi-class multi-label Machine Learning (ML) method XGBoost and the PySSED spectral-energy-distribution fitting algorithm to classify stars into nine different classes, based on their photometric data. The classifier is trained on subsets of the SIMBAD database. Particular challenges are the very high sparsity (large fraction of missing values) of the underlying data as well as the high class imbalance. We discuss the different variables available, such as photometric measurements on the one hand, and indirect predictors such as Galactic position on the other hand.</p><p><strong>Results: </strong>We show the difference in performance when excluding certain variables, and discuss in which contexts which of the variables should be used. Finally, we show that increasing the number of samples of a particular type of star significantly increases the performance of the model for that particular type, while having little to no impact on other types. The accuracy of the main classifier is ∼0.7 with a macro F1 score of 0.61.</p><p><strong>Conclusions: </strong>While the current accuracy of the classifier is not high enough to be reliably used in stellar classification, this work is an initial proof of feasibility for using ML to classify stars based on photometry.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"4 ","pages":"29"},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-28eCollection Date: 2024-01-01DOI: 10.12688/openreseurope.18388.1
Mary Grace Vella
In recent years the EU has become increasingly restrictive in its regulation of irregular migration flows despite its outward sustained discourse of safeguarding human lives and the protection of fundamental rights and freedoms. As part of this restrictive strategy, the EU has invested heavily in the securitisation and the management of migration through the externalisation of its borders. The EU-Turkey Deal struck in 2016 paved the way for further agreements with third countries with poor gender equality and human rights records, such as Libya and Tunisia. This externalisation of migration constitutes an important feature of the New Pact on Migration and Asylum adopted in 2023 with the aim of establishing a common asylum process at EU level. Asylum seekers, particularly girls and women are placed at added risk from these restrictive policies and policies of externalisation, as apart from dangers faced by all asylum seekers, they are at increased risk of human trafficking, sexual abuse and exploitation, and others forms of gender-based violence. Despite offering a short-sighted measure to a complex problem and the adverse humanitarian repercussions and gross human rights violations, particularly on female refugees and asylum seekers, such deals and pacts constitute an integral aspect of the EU's strategic agenda of managing migration through the externalisation of its borders. The article proposes a number of alternative solution-oriented measures which safeguard fundamental human rights and freedoms, in particular the right to asylum for refugee women and girls.
{"title":"Bad Deals and Tragic Pacts: The impact of the EU's management of migration through the externalisation of its borders on 'Women on the Move'.","authors":"Mary Grace Vella","doi":"10.12688/openreseurope.18388.1","DOIUrl":"https://doi.org/10.12688/openreseurope.18388.1","url":null,"abstract":"<p><p>In recent years the EU has become increasingly restrictive in its regulation of irregular migration flows despite its outward sustained discourse of safeguarding human lives and the protection of fundamental rights and freedoms. As part of this restrictive strategy, the EU has invested heavily in the securitisation and the management of migration through the externalisation of its borders. The EU-Turkey Deal struck in 2016 paved the way for further agreements with third countries with poor gender equality and human rights records, such as Libya and Tunisia. This externalisation of migration constitutes an important feature of the New Pact on Migration and Asylum adopted in 2023 with the aim of establishing a common asylum process at EU level. Asylum seekers, particularly girls and women are placed at added risk from these restrictive policies and policies of externalisation, as apart from dangers faced by all asylum seekers, they are at increased risk of human trafficking, sexual abuse and exploitation, and others forms of gender-based violence. Despite offering a short-sighted measure to a complex problem and the adverse humanitarian repercussions and gross human rights violations, particularly on female refugees and asylum seekers, such deals and pacts constitute an integral aspect of the EU's strategic agenda of managing migration through the externalisation of its borders. The article proposes a number of alternative solution-oriented measures which safeguard fundamental human rights and freedoms, in particular the right to asylum for refugee women and girls.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"4 ","pages":"187"},"PeriodicalIF":0.0,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-20eCollection Date: 2023-01-01DOI: 10.12688/openreseurope.15434.2
Carolin Gebauer, Roy Sommer
Life stories play a crucial role in migration discourses: they serve as testimony in journalistic work, form the core of ambassadorial storytelling by non-governmental organizations (NGOs), and inspire collaborative projects initiated by writers seeking to express their solidarity. However, this article argues, drawing on migrants' experiences for such purposes also creates an ethical dilemma: speaking about-or even for-rather than with migrants assigns them a passive role and tends to recycle existing narrative patterns and templates. Starting with a generic distinction between what we call stories of migration (various forms of self-expression granting migrants full authority and control over their narrative) and narratives on migration (external perspectives, e.g., academic, economic, political, and legal approaches, detached from lived experience), we explore the extensive middle ground of hybrid forms between these two extremes- i.e., different kinds of vicarious storytelling-before addressing their ethical implications. We further discuss how the metaphor of the level playing field, a key concept in economics, can be used in transdisciplinary research projects to establish level telling fields (LTFs), i.e., communicative spaces characterized by a fair dialogue on an equal footing for all participants.
{"title":"Beyond Vicarious Storytelling: How Level Telling Fields Could Help Create a Fair Narrative on Migration.","authors":"Carolin Gebauer, Roy Sommer","doi":"10.12688/openreseurope.15434.2","DOIUrl":"https://doi.org/10.12688/openreseurope.15434.2","url":null,"abstract":"<p><p>Life stories play a crucial role in migration discourses: they serve as testimony in journalistic work, form the core of ambassadorial storytelling by non-governmental organizations (NGOs), and inspire collaborative projects initiated by writers seeking to express their solidarity. However, this article argues, drawing on migrants' experiences for such purposes also creates an ethical dilemma: speaking <i>about</i>-or even <i>for</i>-rather than <i>with</i> migrants assigns them a passive role and tends to recycle existing narrative patterns and templates. Starting with a generic distinction between what we call stories of migration (various forms of self-expression granting migrants full authority and control over their narrative) and narratives on migration (external perspectives, <i>e.g.</i>, academic, economic, political, and legal approaches, detached from lived experience), we explore the extensive middle ground of hybrid forms between these two extremes- <i>i.e.</i>, different kinds of vicarious storytelling-before addressing their ethical implications. We further discuss how the metaphor of the level playing field, a key concept in economics, can be used in transdisciplinary research projects to establish level telling fields (LTFs), <i>i.e.</i>, communicative spaces characterized by a fair dialogue on an equal footing for all participants.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"3 ","pages":"10"},"PeriodicalIF":0.0,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-13eCollection Date: 2024-01-01DOI: 10.12688/openreseurope.18226.1
Cristina Claver, Naiara Rodríguez-Ezpeleta, Xabier Irigoien, Oriol Canals
Background: Siphonophores are diverse, globally distributed hydrozoans that play a central role in marine trophic webs worldwide. However, they still constitute an understudied fraction of the open ocean gelatinous taxa, mainly due to challenges related to siphonophore sampling and identification, which have led to a general knowledge gap about their diversity, distribution and abundance.
Methods: Here, we provide a global overview of the oceanic vertical distribution of siphonophores using DNA metabarcoding data from 77 bulk mesozooplankton samples collected at four different depth ranges (0-200, 200-500, 500-1000, 1000-3000 m depth) along the Atlantic, Pacific, and Indian Oceans during the MALASPINA-2010 circumnavigation expedition.
Results: We detected a total of 44 siphonophore species (which represents about one quarter of the described siphonophore species) from which 26 corresponded to Calycophores, 14 to Physonectae and 2 to Cystonectae. Our results suggest wider horizontal and vertical distributions of siphonophore species than previously described, including novel records of some species in certain oceanic basins. Also, we provide insights into the intraspecific variation of widely distributed species. Finally, we show a vertical structuring of siphonophores along the water column; Calycophores (siphonophores without pneumatophores) dominated the epipelagic (from the surface to 200 m depth) and upper mesopelagic layers (from 200 to 500 m depth), while the proportion Physonectids (siphonophores with pneumatophore) notably increased below 500 meters and were dominant at bathypelagic depths (>1000 m depth).
Conclusions: Our results support that the siphonophore community composition is vertically structured. Also, we provide insights into the potential existence of genetic variations within certain species that dominate some ocean basins or depth ranges. To our knowledge, this is the first time that DNA metabarcoding data is retrieved to study siphonophore distribution patterns, and the study provides evidence of the potential of molecular techniques to study the distribution of gelatinous organisms often destroyed in net sampling.
{"title":"Global distribution patterns of siphonophores across horizontal and vertical oceanic gradients.","authors":"Cristina Claver, Naiara Rodríguez-Ezpeleta, Xabier Irigoien, Oriol Canals","doi":"10.12688/openreseurope.18226.1","DOIUrl":"https://doi.org/10.12688/openreseurope.18226.1","url":null,"abstract":"<p><strong>Background: </strong>Siphonophores are diverse, globally distributed hydrozoans that play a central role in marine trophic webs worldwide. However, they still constitute an understudied fraction of the open ocean gelatinous taxa, mainly due to challenges related to siphonophore sampling and identification, which have led to a general knowledge gap about their diversity, distribution and abundance.</p><p><strong>Methods: </strong>Here, we provide a global overview of the oceanic vertical distribution of siphonophores using DNA metabarcoding data from 77 bulk mesozooplankton samples collected at four different depth ranges (0-200, 200-500, 500-1000, 1000-3000 m depth) along the Atlantic, Pacific, and Indian Oceans during the MALASPINA-2010 circumnavigation expedition.</p><p><strong>Results: </strong>We detected a total of 44 siphonophore species (which represents about one quarter of the described siphonophore species) from which 26 corresponded to Calycophores, 14 to Physonectae and 2 to Cystonectae. Our results suggest wider horizontal and vertical distributions of siphonophore species than previously described, including novel records of some species in certain oceanic basins. Also, we provide insights into the intraspecific variation of widely distributed species. Finally, we show a vertical structuring of siphonophores along the water column; Calycophores (siphonophores without pneumatophores) dominated the epipelagic (from the surface to 200 m depth) and upper mesopelagic layers (from 200 to 500 m depth), while the proportion Physonectids (siphonophores with pneumatophore) notably increased below 500 meters and were dominant at bathypelagic depths (>1000 m depth).</p><p><strong>Conclusions: </strong>Our results support that the siphonophore community composition is vertically structured. Also, we provide insights into the potential existence of genetic variations within certain species that dominate some ocean basins or depth ranges. To our knowledge, this is the first time that DNA metabarcoding data is retrieved to study siphonophore distribution patterns, and the study provides evidence of the potential of molecular techniques to study the distribution of gelatinous organisms often destroyed in net sampling.</p>","PeriodicalId":74359,"journal":{"name":"Open research Europe","volume":"4 ","pages":"177"},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}