Open research Europe最新文献

Science can play a pivotal role in providing support to public health policy making processes, and to ensure effective and efficient implementation of policies. This work illustrates how science was integrated into policymaking during the peak of the COVID-19 pandemic, in relation to countries' COVID-19 antigen testing strategies as well as the implementation of Regulation (EU) 2021/953 on the EU Digital COVID Certificate. The lessons learnt during this process as well as the critical steps taken, and concrete recommendations, have been identified and capitalised and turned into a list of science-based advice. The availability of an already established mechanism that can be quickly adapted in case of need, is likely to be highly beneficial in case of a future public health emergency.

Cells that proliferate in confined environments develop mechanical compressive stress, referred to as growth-induced pressure, which inhibits growth and division across various organisms. Recent studies have shown that in these confined spaces, the diffusivity of intracellular nanoparticles decreases. However, the physical mechanisms behind this reduction remain unclear. In this study, we use quantitative phase imaging to measure the refractive index and dry mass density of Saccharomyces cerevisiae cells proliferating under confinement in a microfluidic bioreactor. Our results indicate that the observed decrease in diffusivity could be attributed to the intracellular accumulation of macromolecules. Furthermore, the linear scaling between cell content and growth-induced pressure suggests that the concentrations of macromolecules and osmolytes are maintained proportionally under such pressure in S. cerevisiae.

Background: Human induced pluripotent stem cells (hiPSCs) are pivotal for studying human development, modeling diseases, and advancing regenerative medicine. Effective control of transgene expression is crucial to achieve temporal and quantitative precision in all of these contexts. The doxycycline (dox)-inducible OPTi-OX system, which integrates the Tet-On 3G transactivator and dox-responsive transgene at the hROSA26 and AAVS1 genomic safe harbors (GSHs), respectively, offers a promising solution. Yet, transgene silencing, particularly in hiPSC-derived cardiomyocytes (hiPSC-CMs), limits its utility.

Methods: To address this, we evaluated strategies to enhance dox-inducible transgene expression. We compared two promoters, TRE3VG and T11, for activity and stability, and investigated the addition of a Ubiquitous Chromatin Opening Element (UCOE) to reduce silencing. We also tested relocating the transgene cassette to the CLYBL GSH, and employed sodium butyrate (SB), a histone deacetylase inhibitor, to restore promoter activity. Transgene expression was assessed via flow cytometry and real-time quantitative PCR.

Results: TRE3VG exhibited higher activity than T11, but both were prone to silencing. UCOE did not enhance promoter activity in hiPSCs, but modestly reduced silencing in hiPSC-CMs. Targeting the CLYBL locus improved promoter activity compared to AAVS1 in both hiPSCs and hiPSC-CMs. SB restored activity in silenced inducible promoters within hiPSC-CMs, but compromised hiPSC viability. Unexpectedly, Tet-On 3G was silenced in some clones and could not be reactivated by SB.

Conclusions: These findings underscore the need for integrating multiple strategies, including careful GSH selection, improved cassette design, epigenetic modulation, and clone screening, to develop robust dox-inducible systems that retain functionality during hiPSC differentiation.

MultiOptForest is an open-source software designed to simplify building and solving multi-objective optimization problems for forest planning. It aims to find the optimal portfolio of management regimes that balance the objectives regarding multiple forest ecosystem services and biodiversity. The software flexibly imports data, allowing for the use of a variety of forest simulator outputs. The user provides preference information through a user-friendly graphical interface, where the range of possible values for each objective is provided. MultiOptForest solves the optimization problem producing a set of Pareto optimal solutions, i.e., solutions where none of the objectives can be improved without compromising others. MultiOptForest is versatile enough to design a Pareto optimal forest plan for a small holding to assess management and the trade-off between multiple policy objectives impacting the future development of forests across regions and countries.

The study of planets and small bodies within our Solar System is fundamental for understanding the formation and evolution of the Earth and other planets. Compositional and meteorological studies of the giant planets provide a foundation for understanding the nature of the most commonly observed exoplanets, while spectroscopic observations of the atmospheres of terrestrial planets, moons, and comets provide insights into the past and present-day habitability of planetary environments, and the availability of the chemical ingredients for life. While prior and existing (sub)millimeter observations have led to major advances in these areas, progress is hindered by limitations in the dynamic range, spatial and temporal coverage, as well as sensitivity of existing telescopes and interferometers. Here, we summarize some of the key planetary science use cases that factor into the design of the Atacama Large Aperture Submillimeter Telescope (AtLAST), a proposed 50-m class single dish facility: (1) to more fully characterize planetary wind fields and atmospheric thermal structures, (2) to measure the compositions of icy moon atmospheres and plumes, (3) to obtain detections of new, astrobiologically relevant gases and perform isotopic surveys of comets, and (4) to perform synergistic, temporally-resolved measurements in support of dedicated interplanetary space missions. The improved spatial coverage (several arcminutes), resolution (~ 1.2″ - 12″), bandwidth (several tens of GHz), dynamic range (~ 10 ⁵) and sensitivity (~ 1 mK km s ^-1) required by these science cases would enable new insights into the chemistry and physics of planetary environments, the origins of prebiotic molecules and the habitability of planetary systems in general.

Background: Despite extensive studies of the Mesozoic-Cenozoic magmatic history of Svalbard, little has been done on the Paleozoic magmatism due to fewer available outcrops.

Methods: 2D seismic reflection data were used to study magmatic intrusions in the subsurface of eastern Svalbard.

Results: This work presents seismic evidence for west-dipping, Middle Devonian-Mississippian sills in eastern Spitsbergen, Svalbard. The sills crosscut a late Neoproterozoic Timanian thrust system, which was reworked during Caledonian contraction. The sills are unconformably overlain by relatively undeformed Pennsylvanian-Mesozoic sedimentary rocks and crosscut by Cretaceous dykes of the High Arctic Large Igneous Province. The sills probably intruded along extensional fractures during post-Caledonian reactivation-overprinting of the late Neoproterozoic thrust system. Kimberlitic accessory minerals in exposed contemporaneous intrusions and the chemical composition of chromium spinel grains in Upper Triassic sedimentary rocks in Svalbard suggest that the Middle Devonian-Mississippian intrusions in eastern Spitsbergen show affinities with diamond-rich kimberlites in northwestern Russia. Overall, the sills were emplaced during a regional episode of extension-related Devonian-Carboniferous magmatism in the Northern Hemisphere including the Kola-Dnieper and Yakutsk-Vilyui large igneous provinces.

Conclusions: This work presents the first evidence for extensive Middle Devonian-Mississippian magmatism in Svalbard. These intrusions may be part of the Kola-Dnieper Large Igneous Province and intruded parallel to preexisting, Proterozoic-early Paleozoic orogenic structures. Their strike is inconsistent with a source from a potential mantle plume center in the eastern Barents Sea. Thus, the radiating emplacement pattern of the magmatic intrusions of the Kola-Dnieper Large Igneous Province are not the product of plume-related uplift but of structural inheritance. A similar line of reasoning is successfully applied to intrusions of the Yakutsk-Vilyui and High Arctic large igneous provinces.

This article is aimed at providing a solid empirical basis which can inspire the development of more informed strategies in the field of prevention of undocumented forms of migration across the Mediterranean. Besides, more traditional forms of human smuggling and trafficking, a new phenomenon has emerged especially along the Central Mediterranean route, i.e. trafficking in itinere, which affects people who had originally sought irregular travel services but then ended up in being kidnapped, tortured and kept in detention centres during their migratory path. Therefore, their irregular journey to Europe becomes the only way to survive. The methodology adopted draws from in-depth interviews with experts in Italy - especially prosecutors, law enforcements officers, representatives of NGOs and journalists -, the analysis of institutional reports and sources, as well as biographical accounts provided by migrants. The analysis critically addresses the countereffects produced by the hardening of borders and militarisation of sea routes in the Mediterranean area especially in terms of enhancement of the smuggling industry, increased human rights violations and clandestinization of migrants whose experiences and knowledge are too often underrepresented or misrepresented in the public debate. This results in a negative impact on migration policy-making and on the adoption of more effective measures aimed at addressing the governance of borders and the reception system in the EU. In conclusion, some recommendations are made, which call for a reconsideration of the distinction between economic and political migrants, an enhancement of the right to migrate which can guarantee safer forms of mobility toward the EU, a serious reconsideration of the politics of externalization of European borders with its extremely severe costs in terms of human rights, and a stronger valorisation of migrants' actual needs, expectations and projects in the design of more effective policies aimed at improving the overall EU reception system.

Background: The Faroe margin in the northeastern Atlantic is segmented by margin-orthogonal, WNW-ESE-striking lineaments extending several hundred kilometers out to the continent-ocean transition. Despite several earlier studies speculating that these features are the product of reactivation of pre-Cenozoic basement-seated structures at depth, the thick Cenozoic volcano-sedimentary sequences deposited along the margin mask the underburden, thus rendering the identification and interpretation of such structures and resolving the pre-Cenozoic history of the area challenging. The present study documents for the first time the existence of margin-orthogonal basement-seated thrust systems and describes their detailed geometry, kinematics, and tectonic evolution.

Methods: We interpreted basement-seated tectonic structures on seismic reflection data from TGS on the Faroe Platform and the Wyville-Thomson and Munkagrunnur ridges using the newly established seismic facies of major thrust systems.

Results: The data show that the Wyville-Thomson Ridge, Munkagrunnur Ridge, and Faroe Platform are cored by WNW-ESE-striking thrust systems hundreds of kilometers long and 30-50 km wide, showing dominantly top-SSW kinematics. The thrusts were reworked into NE-SW-striking folds during the Caledonian Orogeny and controlled the formation of Caledonian thrusts, which in turn controlled the formation of post-Caledonian faults. The pre-Caledonian nature of the WNW-ESE-striking thrusts and their geometry and kinematics suggest a relationship with late Paleoproterozoic Laxfordian shear zones onshore northern Scotland and the continuation of the coeval Nagssugtoqidian Orogen in southeastern Greenland, the Ammassalik Belt. The thrust systems also align with the Tornquist Zone in eastern Europe and the North Sea, thus suggesting either that they controlled the formation of the Tornquist Zone or a possibly much longer (Paleoproterozoic?) tectonic history for the Tornquist Zone.

Conclusions: The Faroe Island margin is crosscut by late Paleoproterozoic Laxfordian-Nagssugtoqidian thrust systems, which controlled further tectonic development of the margin.

Background: Dictyostelia are soil amoebas that aggregate to form fruiting bodies with spores and stalk cells in response to starvation. Where known, species across the dictyostelid phylogeny use secreted cAMP, detected by cAMP receptors (cARs) to induce the differentiation of spores and to organize fruiting body construction. However, recent deletion of the single cAR of Polyspondylium violaceum (Pvio) left both its fruiting bodies and spores intact.

Methods: To investigate whether Pvio sporulation can occur in the absence of secreted cAMP and to explore alternative inducers in a bioassay , three prespore genes were identified and gene fusions of their promoters with the LacZ reporter gene were transformed into Pvio cells. After assessing the spatial expression pattern of the genes and the stage at which prespore gene expression initiated, the effect of cAMP and other Dictyostelium discoideum ( Ddis) signal molecules were tested on prespore gene expression in vitro.

Results: Pvio genes g4562 (psp1), g2696 (psp2) and g2380 (psp3) were identified as homologs of Ddis spore coat genes. They were first expressed around 4 h of starvation in aggregation centres and later in the posterior 4/5 ^th of emerging sorogens and the spore head of early fruiting bodies. Cells from dissociated 4 h aggregates and shaken in suspension for 6 h increased prespore- LacZ reporter activity 4-fold for psp1 and 6-fold for psp2, but this increase was at least 5-fold higher when cells were plated on solid substratum for 6 h to develop normally. cAMP had no effect on prespore gene induction and neither had the Pvio chemoattractant glorin nor the Ddis chemoattractants and differentiation inducers folate, c-di-GMP, DIF-1, GABA, cGMP and 8Br-cAMP.

Conclusions: The Pvio lineage uniquely evolved a novel genetic network for synthesis, detection and processing of the signal that triggers its main survival strategy.

Background: Elastic deformations of gravitating cylindrical bodies are relevant for state-of-the-art photonic experiments, as they affect the physical properties of materials under consideration, impacting wave propagation. This is of key importance for a recently planned experiment to explore the influence of the gravitational field on entangled photons propagating in waveguides. The purpose of this work is to determine these elastic deformations as functions of temperature, pressure, and of the gravitational field. We thus determine the deformations of the body due to changes of the gravitational field, and obtain stringent bounds on the control of temperature and pressure so that the effects of the associated elastic deformations on the photons propagating in a waveguide are smaller than the phase shifts associated with the change of the gravitational field.

Methods: We use the methods of linear elasticity, including thermoelasticity, to determine the stresses and strains of the medium. For this, the symmetry of the cylinder allows us to solve the problem by using Mitchell's solutions of the equations satisfied by the Airy functions. The boundary conditions are implemented by an approximation of the Hertz contact method.

Results: We calculate the displacements, the stresses and strains for several classes of boundary conditions, and give explicit solutions for a number of physically motivated configurations. The influence of the resulting deformations on the planned GRAVITES experiment is determined.

Conclusions: The results are relevant for fiber interferometry experiments sensitive to the effects of the gravitational field on photon propagation. Our calculations give stringent bounds on the environmental variables, which need to be controlled in such experiments.