Pub Date : 2024-07-25DOI: 10.3390/agriculture14081226
Hao Wang, Lixin Zhang, Bao Liu
The efficient operation of smart farms relies on the precise monitoring of farm environmental information, necessitating the deployment of a large number of wireless sensors. These sensors must be integrated with their specific locations within the fields to ensure data accuracy. Therefore, efficiently and rapidly determining the positions of sensor nodes presents a significant challenge. To address this issue, this paper proposes a hybrid optimization DV-Hop localization algorithm based on the chaotic crested porcupine optimizer. The algorithm leverages the received signal strength indicator, combined with node hierarchical values, to achieve graded processing of the minimum number of hops. Polynomial fitting methods are employed to reduce the estimation distance error from the beacon nodes to unknown nodes. Finally, the chaotic optimization crested porcupine optimizer is designed for intelligent optimization. Simulation experiments verify the proposed algorithm’s localization performance across different monitoring areas, varying beacon node ratios, and assorted communication radii. The simulation results demonstrate that the proposed algorithm effectively enhances node localization accuracy and significantly reduces localization errors compared to the results for other algorithms. In future work, we plan to consider the impact of algorithm complexity on the lifespan of wireless sensor networks and to further evaluate the algorithm in a pH monitoring system for farmland.
{"title":"Research and Design of a Hybrid DV-Hop Algorithm Based on the Chaotic Crested Porcupine Optimizer for Wireless Sensor Localization in Smart Farms","authors":"Hao Wang, Lixin Zhang, Bao Liu","doi":"10.3390/agriculture14081226","DOIUrl":"https://doi.org/10.3390/agriculture14081226","url":null,"abstract":"The efficient operation of smart farms relies on the precise monitoring of farm environmental information, necessitating the deployment of a large number of wireless sensors. These sensors must be integrated with their specific locations within the fields to ensure data accuracy. Therefore, efficiently and rapidly determining the positions of sensor nodes presents a significant challenge. To address this issue, this paper proposes a hybrid optimization DV-Hop localization algorithm based on the chaotic crested porcupine optimizer. The algorithm leverages the received signal strength indicator, combined with node hierarchical values, to achieve graded processing of the minimum number of hops. Polynomial fitting methods are employed to reduce the estimation distance error from the beacon nodes to unknown nodes. Finally, the chaotic optimization crested porcupine optimizer is designed for intelligent optimization. Simulation experiments verify the proposed algorithm’s localization performance across different monitoring areas, varying beacon node ratios, and assorted communication radii. The simulation results demonstrate that the proposed algorithm effectively enhances node localization accuracy and significantly reduces localization errors compared to the results for other algorithms. In future work, we plan to consider the impact of algorithm complexity on the lifespan of wireless sensor networks and to further evaluate the algorithm in a pH monitoring system for farmland.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"59 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141805855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.3390/agriculture14081224
Zhuoya Tian, Xicong Wang, Zekui Lei, Zhenhong Qi, Zhe Liu
The income effect of rice–crayfish co-culture technology (RCT) is directly related to rate of adoption of farmers and the process of China’s green development of agriculture. The aim of this study is to explore the income effect and income growth mechanism of rice–crayfish co-culture technology from the perspective of continuous adoption. With the treatment effect model (TEM), this paper empirically analyzes the income effect and income-generating mechanisms of RCT using field survey data from 736 farmers in the Jianghan Plain. As a result of this study, it was discovered that RCT will increase farmers’ net agricultural income by RMB 83,430 if they continue to adopt it. Further examinations indicate that the optimal adoption period for RCT is four and a half years. Additionally, it has also been shown that non-farm employment positively moderates the relationship between continuous adoption of RCT and net agricultural income. Farmers who participate in non-farm employment and continue to adopt the RCT will experience an increase in net agricultural income by RMB 104,510. Therefore, our results suggest that it is necessary to encourage farmers to continuously adopt RCT and actively participate in non-farm employment to enhance the income effect of RCT.
{"title":"A Study of the Income Effect of Continuous Adoption of Rice–Crayfish Co-Culture Technology: Based on the Moderating Effect of Non-Farm Employment","authors":"Zhuoya Tian, Xicong Wang, Zekui Lei, Zhenhong Qi, Zhe Liu","doi":"10.3390/agriculture14081224","DOIUrl":"https://doi.org/10.3390/agriculture14081224","url":null,"abstract":"The income effect of rice–crayfish co-culture technology (RCT) is directly related to rate of adoption of farmers and the process of China’s green development of agriculture. The aim of this study is to explore the income effect and income growth mechanism of rice–crayfish co-culture technology from the perspective of continuous adoption. With the treatment effect model (TEM), this paper empirically analyzes the income effect and income-generating mechanisms of RCT using field survey data from 736 farmers in the Jianghan Plain. As a result of this study, it was discovered that RCT will increase farmers’ net agricultural income by RMB 83,430 if they continue to adopt it. Further examinations indicate that the optimal adoption period for RCT is four and a half years. Additionally, it has also been shown that non-farm employment positively moderates the relationship between continuous adoption of RCT and net agricultural income. Farmers who participate in non-farm employment and continue to adopt the RCT will experience an increase in net agricultural income by RMB 104,510. Therefore, our results suggest that it is necessary to encourage farmers to continuously adopt RCT and actively participate in non-farm employment to enhance the income effect of RCT.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"44 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141805485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sesuvium portulacastrum L. is a dicotyledonous halophyte belonging to the family Aizoaceae. Its young leaves are highly nutritious, and many ecotypes are used as leafy vegetable and medicinal crops. Additionally, due to their tolerance to soil salinity, flooding, and high temperatures, some ecotypes are used for the remediation of saline soils. As a result, there is an increasing need for a large number of disease-free S. portulacastrum propagules. This study developed an efficient protocol for the regeneration of S. portulacastrum through indirect shoot organogenesis. Leaf explants were cultured on Murashige and Skoog basal medium supplemented with different concentrations of zeatin (ZT) and indole-3-acetic acid (IAA). Callus was induced in all explants cultured with 1.5 mg/L ZT only or 1.5 mg/L ZT with 0.5 mg/L IAA. The callus was cut into small pieces and cultured on the same medium on which it was initially induced. ZT at 1.5 mg/L induced 73.7% of callus pieces to produce adventitious shoots, and the shoot numbers per callus piece were up to 20. To improve the in vitro rooting of adventitious shoots, commonly known as microshoots or microcuttings, an endophytic fungus, Cladosporium ‘BF-F’, was inoculated onto the rooting medium. ‘BF-F’ substantially enhanced rooting and plantlet growth, as the root numbers were three times more and plantlet heights were 70% greater than those without ‘BF-F’ inoculation. To detect the genes involved in the enhanced rooting and plantlet growth, qRT-PCR analysis was performed. Results showed that genes related to auxin responses and nitrogen uptake and metabolism were highly upregulated in ‘BF-F’-inoculated plantlets. Plants inoculated with ‘BF-F’ grew vigorously after being transplanted into a sand–soil substrate. Thus, this study not only established an efficient protocol for the regeneration of S. portulacastrum but also developed a novel method for improving the rooting of microshoots and plantlet growth. The established propagation system could be used for producing a large number of S. portulacastrum plantlets for commercial use and also for genetic transformation.
Sesuvium portulacastrum L.是一种双子叶卤叶植物,属于豆科(Aizoaceae)。它的嫩叶营养丰富,许多生态型被用作叶菜和药用作物。此外,由于耐盐碱、耐洪水和耐高温,一些生态型被用于盐碱土的修复。因此,人们越来越需要大量无病的 S. portulacastrum 繁殖体。本研究开发了一种通过间接芽器官发生再生 S. portulacastrum 的有效方法。叶片外植体在添加了不同浓度玉米素(ZT)和吲哚-3-乙酸(IAA)的 Murashige 和 Skoog 基础培养基上培养。只用 1.5 毫克/升玉米素或 1.5 毫克/升玉米素加 0.5 毫克/升吲哚-3-乙酸培养的所有外植体都能诱导出胼胝体。将胼胝体切成小块,并在最初诱导胼胝体的相同培养基上进行培养。1.5 毫克/升的 ZT 能诱导 73.7% 的胼胝体产生不定芽,每个胼胝体的不定芽数量可达 20 个。为了提高不定芽(通常称为微芽或微枝)的离体生根率,在生根培养基中接种了一种内生真菌,Cladosporium 'BF-F'。与未接种'BF-F'的培养基相比,'BF-F'大大提高了生根和小植株的生长速度,生根数量增加了三倍,小植株高度增加了 70%。为了检测参与生根和小植株生长增强的基因,进行了 qRT-PCR 分析。结果表明,在接种'BF-F'的小植株中,与辅助素反应和氮吸收及代谢有关的基因高度上调。接种了'BF-F'的植株在移栽到沙土基质中后生长旺盛。因此,本研究不仅为 S. portulacastrum 的再生建立了一个有效的方案,还开发了一种新的方法来改善小芽的生根和小植株的生长。所建立的繁殖系统可用于生产大量商业用途的 S. portulacastrum 小植株,也可用于基因转化。
{"title":"Regeneration of Sesuvium portulacastrum through Indirect Shoot Organogenesis and Influence of an Endophytic Fungus on Rooting of Microshoots","authors":"Xiuli Jiang, Dan Wang, Jianjun Chen, Weihong He, Boya Zhou, Ziling Li, Ling-Yan Chen, Donghui Peng, Qiang Chen, Xiangying Wei","doi":"10.3390/agriculture14081221","DOIUrl":"https://doi.org/10.3390/agriculture14081221","url":null,"abstract":"Sesuvium portulacastrum L. is a dicotyledonous halophyte belonging to the family Aizoaceae. Its young leaves are highly nutritious, and many ecotypes are used as leafy vegetable and medicinal crops. Additionally, due to their tolerance to soil salinity, flooding, and high temperatures, some ecotypes are used for the remediation of saline soils. As a result, there is an increasing need for a large number of disease-free S. portulacastrum propagules. This study developed an efficient protocol for the regeneration of S. portulacastrum through indirect shoot organogenesis. Leaf explants were cultured on Murashige and Skoog basal medium supplemented with different concentrations of zeatin (ZT) and indole-3-acetic acid (IAA). Callus was induced in all explants cultured with 1.5 mg/L ZT only or 1.5 mg/L ZT with 0.5 mg/L IAA. The callus was cut into small pieces and cultured on the same medium on which it was initially induced. ZT at 1.5 mg/L induced 73.7% of callus pieces to produce adventitious shoots, and the shoot numbers per callus piece were up to 20. To improve the in vitro rooting of adventitious shoots, commonly known as microshoots or microcuttings, an endophytic fungus, Cladosporium ‘BF-F’, was inoculated onto the rooting medium. ‘BF-F’ substantially enhanced rooting and plantlet growth, as the root numbers were three times more and plantlet heights were 70% greater than those without ‘BF-F’ inoculation. To detect the genes involved in the enhanced rooting and plantlet growth, qRT-PCR analysis was performed. Results showed that genes related to auxin responses and nitrogen uptake and metabolism were highly upregulated in ‘BF-F’-inoculated plantlets. Plants inoculated with ‘BF-F’ grew vigorously after being transplanted into a sand–soil substrate. Thus, this study not only established an efficient protocol for the regeneration of S. portulacastrum but also developed a novel method for improving the rooting of microshoots and plantlet growth. The established propagation system could be used for producing a large number of S. portulacastrum plantlets for commercial use and also for genetic transformation.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"23 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141803086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.3390/agriculture14081228
Beata Kowalska, M. Szczech, A. Lisek
The evaluation of the potentiality of lactic acid bacteria (LAB) strains isolated from fermented products to inhibit Botrytis cinerea and Escherichia coli O157:H7 growth on spinach and lettuce was conducted. From a total of forty LAB strains tested, three were selected due to their high inhibitory effect on plant pathogenic fungi. The identification of these isolates based on a 16S rRNA gene fragment sequence analysis confirmed the genus of Levilactobacillus sp. and Lactiplantibacillus sp. An effective method of coating LAB isolates on the lettuce and spinach surface was developed. The leaves were immersed in bacterial suspension (5.0 × 106 cfu mL−1) for 4 s and drained on tissue paper. LAB survived on lettuce and spinach leaves for 8 days at 6 log10 cfu g−1. Additionally, these bacteria decreased the number of filamentous fungi on the leaves. These isolates were found to inhibit the growth of B. cinerea and E. coli O157:H7 in vitro conditions in growing microbiological media. Their efficacy was confirmed in vivo conditions. These isolates inhibited the development of grey mould caused by B. cinerea on lettuce leaves. Two LAB isolates reduced the abundance of the pathogenic bacterium E. coli on spinach leaves by about 0.7 log10 cfu g−1. In glasshouse conditions, LAB stimulated the growth of examined plants. The lactic acid bacteria used in this study showed the capacity to be used as possible alternatives to chemical compounds in the protection of leafy vegetables against grey mould and for a decrease in E. coli O157:H7 contamination.
{"title":"Inhibition of Botrytis cinerea and Escherichia coli by Lactic Acid Bacteria on Leafy Vegetables","authors":"Beata Kowalska, M. Szczech, A. Lisek","doi":"10.3390/agriculture14081228","DOIUrl":"https://doi.org/10.3390/agriculture14081228","url":null,"abstract":"The evaluation of the potentiality of lactic acid bacteria (LAB) strains isolated from fermented products to inhibit Botrytis cinerea and Escherichia coli O157:H7 growth on spinach and lettuce was conducted. From a total of forty LAB strains tested, three were selected due to their high inhibitory effect on plant pathogenic fungi. The identification of these isolates based on a 16S rRNA gene fragment sequence analysis confirmed the genus of Levilactobacillus sp. and Lactiplantibacillus sp. An effective method of coating LAB isolates on the lettuce and spinach surface was developed. The leaves were immersed in bacterial suspension (5.0 × 106 cfu mL−1) for 4 s and drained on tissue paper. LAB survived on lettuce and spinach leaves for 8 days at 6 log10 cfu g−1. Additionally, these bacteria decreased the number of filamentous fungi on the leaves. These isolates were found to inhibit the growth of B. cinerea and E. coli O157:H7 in vitro conditions in growing microbiological media. Their efficacy was confirmed in vivo conditions. These isolates inhibited the development of grey mould caused by B. cinerea on lettuce leaves. Two LAB isolates reduced the abundance of the pathogenic bacterium E. coli on spinach leaves by about 0.7 log10 cfu g−1. In glasshouse conditions, LAB stimulated the growth of examined plants. The lactic acid bacteria used in this study showed the capacity to be used as possible alternatives to chemical compounds in the protection of leafy vegetables against grey mould and for a decrease in E. coli O157:H7 contamination.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"36 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141804123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.3390/agriculture14081225
Rui-Feng Wang, W. Su
The potato is a key crop in addressing global hunger, and deep learning is at the core of smart agriculture. Applying deep learning (e.g., YOLO series, ResNet, CNN, LSTM, etc.) in potato production can enhance both yield and economic efficiency. Therefore, researching efficient deep learning models for potato production is of great importance. Common application areas for deep learning in the potato production chain, aimed at improving yield, include pest and disease detection and diagnosis, plant health status monitoring, yield prediction and product quality detection, irrigation strategies, fertilization management, and price forecasting. The main objective of this review is to compile the research progress of deep learning in various processes of potato production and to provide direction for future research. Specifically, this paper categorizes the applications of deep learning in potato production into four types, thereby discussing and introducing the advantages and disadvantages of deep learning in the aforementioned fields, and it discusses future research directions. This paper provides an overview of deep learning and describes its current applications in various stages of the potato production chain.
{"title":"The Application of Deep Learning in the Whole Potato Production Chain: A Comprehensive Review","authors":"Rui-Feng Wang, W. Su","doi":"10.3390/agriculture14081225","DOIUrl":"https://doi.org/10.3390/agriculture14081225","url":null,"abstract":"The potato is a key crop in addressing global hunger, and deep learning is at the core of smart agriculture. Applying deep learning (e.g., YOLO series, ResNet, CNN, LSTM, etc.) in potato production can enhance both yield and economic efficiency. Therefore, researching efficient deep learning models for potato production is of great importance. Common application areas for deep learning in the potato production chain, aimed at improving yield, include pest and disease detection and diagnosis, plant health status monitoring, yield prediction and product quality detection, irrigation strategies, fertilization management, and price forecasting. The main objective of this review is to compile the research progress of deep learning in various processes of potato production and to provide direction for future research. Specifically, this paper categorizes the applications of deep learning in potato production into four types, thereby discussing and introducing the advantages and disadvantages of deep learning in the aforementioned fields, and it discusses future research directions. This paper provides an overview of deep learning and describes its current applications in various stages of the potato production chain.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"56 49","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141804896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.3390/agriculture14081227
Xu Xiao, Yaonan Wang, Bing Zhou, Yiming Jiang
In order to meet the demand of the intelligent and efficient picking of fresh citrus fruit in a natural environment, a flexible and independent picking method of fresh citrus fruit based on picking pattern recognition was proposed. The convolutional attention (CA) mechanism was added in the YOLOv7 network model. This makes the model pay more attention to the citrus fruit region, reduces the interference of some redundant information in the background and feature maps, effectively improves the recognition accuracy of the YOLOv7 network model, and reduces the detection error of the hand region. According to the physical parameters of the citrus fruit and stem, an end-effector suitable for picking citrus fruit was designed, which effectively reduced the damage during the picking of citrus fruit. According to the actual distribution of citrus fruits in the natural environment, a citrus fruit-picking task planning model was established, so that the adaptability of the flexible handle can make up for the inaccuracy of the deep learning method to a certain extent when the end-effector picks fruits independently. Finally, on the basis of integrating the key components of the picking robot, a production test was carried out in a standard citrus orchard. The experimental results show that the success rate of the citrus-picking robot arm is 87.15%, and the success rate of picking in the natural field environment is 82.4%, which is better than the success rate of 80% of the market picking robot. In the picking experiment, the main reason for the unsuccessful positioning of citrus fruits is that the position of citrus fruits is beyond the picking range of the end-effector, and the motion parameters of the robot arm joint will produce errors, affecting the motion accuracy of the robot arm, leading to the failure of picking. This study can provide technical support for the exploration and application of the intelligent fruit-picking mode.
{"title":"Flexible Hand Claw Picking Method for Citrus-Picking Robot Based on Target Fruit Recognition","authors":"Xu Xiao, Yaonan Wang, Bing Zhou, Yiming Jiang","doi":"10.3390/agriculture14081227","DOIUrl":"https://doi.org/10.3390/agriculture14081227","url":null,"abstract":"In order to meet the demand of the intelligent and efficient picking of fresh citrus fruit in a natural environment, a flexible and independent picking method of fresh citrus fruit based on picking pattern recognition was proposed. The convolutional attention (CA) mechanism was added in the YOLOv7 network model. This makes the model pay more attention to the citrus fruit region, reduces the interference of some redundant information in the background and feature maps, effectively improves the recognition accuracy of the YOLOv7 network model, and reduces the detection error of the hand region. According to the physical parameters of the citrus fruit and stem, an end-effector suitable for picking citrus fruit was designed, which effectively reduced the damage during the picking of citrus fruit. According to the actual distribution of citrus fruits in the natural environment, a citrus fruit-picking task planning model was established, so that the adaptability of the flexible handle can make up for the inaccuracy of the deep learning method to a certain extent when the end-effector picks fruits independently. Finally, on the basis of integrating the key components of the picking robot, a production test was carried out in a standard citrus orchard. The experimental results show that the success rate of the citrus-picking robot arm is 87.15%, and the success rate of picking in the natural field environment is 82.4%, which is better than the success rate of 80% of the market picking robot. In the picking experiment, the main reason for the unsuccessful positioning of citrus fruits is that the position of citrus fruits is beyond the picking range of the end-effector, and the motion parameters of the robot arm joint will produce errors, affecting the motion accuracy of the robot arm, leading to the failure of picking. This study can provide technical support for the exploration and application of the intelligent fruit-picking mode.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"42 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141805624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-25DOI: 10.3390/agriculture14081223
Ruimin Li, Zixuan Xia, Bo You, Bowen Shi, Jing Fu
Atmospheric volatile organic compounds (VOCs), such as olefins and aromatics, released from synthetic chemical pesticide sprays can increase regional air pollution, public health risks, and food security risks. However, significant uncertainties remain regarding the measurement methods and chemical profiles of VOC emissions. Using an agricultural city, Changchun City in Northeast China, as a case study, we quantified real-time concentration and composition data based on online monitoring instruments for the year 2023. This study optimized data collection methods for emission factors and activity levels and developed a high-precision emission inventory of VOCs in pesticides at the city scale. The emission factors for VOCs from the seven categories of pesticides were estimated as follows: 78 g/kg (nicosulfuron and atrazine, oil-dispersible [OD] and suspension emulsion [SE], respectively), 4 g/kg (chlorpyrifos and indoxair conditioningarb, suspension concentrate [SC]), 5 g/kg (fluopicolide and propamocarb hydrochloride, SC), 217 g/kg (MCPA-dimethylammonium, aqueous solution [AS]), 34 g/kg (glyphosate, AS), 575 g/kg (beta-cypermethrin and malathion, emulsifiable concentrate [EC]), and 122 g/kg (copper abietate, emulsion in water [EW]), depending on the pesticide formulation components and formulation types. The orchard insecticide exhibited the highest emission factors among all pesticides owing to its emulsifiable concentrate formulation and 80% content of inactive ingredients (both factors contribute to the high content of organic solvents in the pesticide). The major components of VOC emissions from pesticide spraying were halocarbons (27–44%), oxygenated VOCs (OVOCs) (25–38%), and aromatic hydrocarbons (15–28%). The total VOC emissions from pesticide spraying in the Changchun region accounted for 10.6 t, with Yushu City contributing 28% of the VOC emissions and Gongzhuling City and Dehui City contributing 18.7% and 16.0%, respectively. Herbicides were the main contributors to VOC emissions because of their high emission factors and extensive use in fields (used for spraying maize and rice, the main crops in Changchun City). May and June exhibited the highest VOC emissions from pesticide application, with May accounting for 57.0% of annual pesticide emissions, predominantly from herbicides (95.1%), followed by insecticides (4.9%). June accounted for 30.1% of the annual pesticide emissions, with herbicides being the largest contributor of VOC emissions. An emission inventory of VOC with a monthly scale and spatial grid resolutions of 0.083° and 0.5° in 2023 was developed. These emission factors and inventories of pesticide applications provide valuable information for air quality modeling. This study also provides an important scientific basis for enhancing regional air quality and mitigating the environmental impact of pesticide use in major grain-producing areas.
{"title":"Volatile Organic Compound Emission Inventory for Pesticide Spraying in an Agricultural City of Northeast China: Real-Time Monitoring and Method Optimization","authors":"Ruimin Li, Zixuan Xia, Bo You, Bowen Shi, Jing Fu","doi":"10.3390/agriculture14081223","DOIUrl":"https://doi.org/10.3390/agriculture14081223","url":null,"abstract":"Atmospheric volatile organic compounds (VOCs), such as olefins and aromatics, released from synthetic chemical pesticide sprays can increase regional air pollution, public health risks, and food security risks. However, significant uncertainties remain regarding the measurement methods and chemical profiles of VOC emissions. Using an agricultural city, Changchun City in Northeast China, as a case study, we quantified real-time concentration and composition data based on online monitoring instruments for the year 2023. This study optimized data collection methods for emission factors and activity levels and developed a high-precision emission inventory of VOCs in pesticides at the city scale. The emission factors for VOCs from the seven categories of pesticides were estimated as follows: 78 g/kg (nicosulfuron and atrazine, oil-dispersible [OD] and suspension emulsion [SE], respectively), 4 g/kg (chlorpyrifos and indoxair conditioningarb, suspension concentrate [SC]), 5 g/kg (fluopicolide and propamocarb hydrochloride, SC), 217 g/kg (MCPA-dimethylammonium, aqueous solution [AS]), 34 g/kg (glyphosate, AS), 575 g/kg (beta-cypermethrin and malathion, emulsifiable concentrate [EC]), and 122 g/kg (copper abietate, emulsion in water [EW]), depending on the pesticide formulation components and formulation types. The orchard insecticide exhibited the highest emission factors among all pesticides owing to its emulsifiable concentrate formulation and 80% content of inactive ingredients (both factors contribute to the high content of organic solvents in the pesticide). The major components of VOC emissions from pesticide spraying were halocarbons (27–44%), oxygenated VOCs (OVOCs) (25–38%), and aromatic hydrocarbons (15–28%). The total VOC emissions from pesticide spraying in the Changchun region accounted for 10.6 t, with Yushu City contributing 28% of the VOC emissions and Gongzhuling City and Dehui City contributing 18.7% and 16.0%, respectively. Herbicides were the main contributors to VOC emissions because of their high emission factors and extensive use in fields (used for spraying maize and rice, the main crops in Changchun City). May and June exhibited the highest VOC emissions from pesticide application, with May accounting for 57.0% of annual pesticide emissions, predominantly from herbicides (95.1%), followed by insecticides (4.9%). June accounted for 30.1% of the annual pesticide emissions, with herbicides being the largest contributor of VOC emissions. An emission inventory of VOC with a monthly scale and spatial grid resolutions of 0.083° and 0.5° in 2023 was developed. These emission factors and inventories of pesticide applications provide valuable information for air quality modeling. This study also provides an important scientific basis for enhancing regional air quality and mitigating the environmental impact of pesticide use in major grain-producing areas.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"59 23","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141804725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-24DOI: 10.3390/agriculture14081217
Yongzhe Zhang, Xinfeng Wang, Yuzhe Tang, Linjing Wang, Rui Han, Xi Qiao, Fanghao Wan, Wanqiang Qian, Conghui Liu
Investigations and research on the giant African snail (GAS) mainly focus on Achatina fulica. However, in recent years, a more harmful GAS, Achatina immaculata, has been reported. In order to understand the invasive status of A. immaculata in China, we take Shenzhen, a coastal city in Southern China, as an example to carry out an ecological survey on the field populations of the two species of GAS in various districts. We explore the differences in the invasive characteristics of the two species of snails in terms of their dietary intake, cold adaptation and drought resistance. The results indicate that, based on the phylogenetic tree analysis, more than half of the sampled snails exhibit higher similarity to A. immaculata. The number of wild A. immaculata is significantly greater than that of A. fulica, and 70.64% of the 746 GAS are A. immaculata. At the same time, it is also found that the maximum shell length of A. immaculata is 135.83 mm, with an average shell length of 76.00 mm, which is significantly different from the average shell length of A. fulica (56.57 mm, p < 0.01). The food intake assay shows that there is no difference in the food preferences of the two species, but the food demand of A. immaculata is significantly greater than that of A. fulica (2.32 fold, p < 0.01). In the cold adaptation assay, A. immaculata recovers from the cold dormancy state significantly faster than A. fulica (1.92 fold, p < 0.05), and the speed with which A. immaculata enters the dormancy state in the drought environment is significantly slower than that of A. fulica (0.706 fold, p < 0.05). With the characteristics of a large body size, large food intake and strong resistance to cold and drought resistance, A. immaculata has the potential to be dominant in competition with A. fulica in the same ecological niche, and it has become the main invasive species of GAS in Shenzhen.
对非洲大蜗牛(GAS)的调查和研究主要集中在 Achatina fulica 上。但近年来,一种危害性更大的非洲大蜗牛--非洲大蜗牛(Achatina immaculata)也有报道。为了了解A. immaculata在中国的入侵状况,我们以华南沿海城市深圳为例,对这两种非洲大蜗牛在各区的野外种群进行了生态调查。我们从食性、寒冷适应性和抗旱性等方面探讨了两种蜗牛入侵特征的差异。结果表明,根据系统发生树分析,一半以上的采样蜗牛与 A. immaculata 表现出较高的相似性。野生 A. immaculata 的数量明显多于 A. fulica,在 746 个 GAS 中,70.64% 为 A. immaculata。同时还发现,A. immaculata 的最大壳长为 135.83 毫米,平均壳长为 76.00 毫米,与 A. fulica 的平均壳长(56.57 毫米,P < 0.01)有显著差异。摄食试验表明,两个物种对食物的喜好没有差异,但A. immaculata对食物的需求量明显高于A. fulica(2.32倍,p < 0.01)。在寒冷适应试验中,金花绣线菊从寒冷休眠状态恢复的速度明显快于富贵竹(1.92 倍,p < 0.05),而金花绣线菊在干旱环境中进入休眠状态的速度明显慢于富贵竹(0.706 倍,p < 0.05)。由于具有体型大、食量大、抗寒性和抗旱性强等特点,金花绣线菊有可能在与福寿螺在同一生态位的竞争中占据优势地位,并已成为深圳地区豚鼠的主要入侵物种。
{"title":"An Investigation and Invasiveness Analysis of Two Species of Giant African Snail in a Coastal City of Southern China","authors":"Yongzhe Zhang, Xinfeng Wang, Yuzhe Tang, Linjing Wang, Rui Han, Xi Qiao, Fanghao Wan, Wanqiang Qian, Conghui Liu","doi":"10.3390/agriculture14081217","DOIUrl":"https://doi.org/10.3390/agriculture14081217","url":null,"abstract":"Investigations and research on the giant African snail (GAS) mainly focus on Achatina fulica. However, in recent years, a more harmful GAS, Achatina immaculata, has been reported. In order to understand the invasive status of A. immaculata in China, we take Shenzhen, a coastal city in Southern China, as an example to carry out an ecological survey on the field populations of the two species of GAS in various districts. We explore the differences in the invasive characteristics of the two species of snails in terms of their dietary intake, cold adaptation and drought resistance. The results indicate that, based on the phylogenetic tree analysis, more than half of the sampled snails exhibit higher similarity to A. immaculata. The number of wild A. immaculata is significantly greater than that of A. fulica, and 70.64% of the 746 GAS are A. immaculata. At the same time, it is also found that the maximum shell length of A. immaculata is 135.83 mm, with an average shell length of 76.00 mm, which is significantly different from the average shell length of A. fulica (56.57 mm, p < 0.01). The food intake assay shows that there is no difference in the food preferences of the two species, but the food demand of A. immaculata is significantly greater than that of A. fulica (2.32 fold, p < 0.01). In the cold adaptation assay, A. immaculata recovers from the cold dormancy state significantly faster than A. fulica (1.92 fold, p < 0.05), and the speed with which A. immaculata enters the dormancy state in the drought environment is significantly slower than that of A. fulica (0.706 fold, p < 0.05). With the characteristics of a large body size, large food intake and strong resistance to cold and drought resistance, A. immaculata has the potential to be dominant in competition with A. fulica in the same ecological niche, and it has become the main invasive species of GAS in Shenzhen.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"23 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141806061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-24DOI: 10.3390/agriculture14081212
Wuhua Zhang, Naiyu Zhang, Qi Qin, Xiaoying Zhang, Jinzhu Zhang, Tao Yang, Yifei Zhang, Jie Dong, Daidi Che
Roses, a popular ornamental crop, often face various abiotic stresses during growth and development, such as cold, drought, and salinity. Rosa multiflora is a commonly used rootstock and exhibits strong resistance to both biotic and abiotic stresses, making it an ideal material for studying mechanisms for resistance. Among the largest plant families, MYB transcription factors play a crucial role in plant abiotic stresses. Our previous research has indicated that RmMYB44 could be involved in the low-temperature response of R. multiflora. This study further investigated RmMYB44, revealing that its expression levels were upregulated in response to chilling, drought, and salt stress. The results suggested its potential role as a key transcription factor in plant resistance to abiotic stresses. Additionally, RmMYB44 encoded a nuclear-localized protein without the self-activating function. The overexpression of RmMYB44 in tobacco plants enhanced the resistance to cold, drought, and salt stresses, as evidenced by the improved growth compared to wild-type (WT) plants under conditions of 4 °C, 30% water-holding capacity, and 200 mM of NaCl, respectively. Moreover, in overexpression tobacco plants, the levels of hydrogen peroxide and malondialdehyde (MDA) were significantly reduced; and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT); as well as the proline content and the expression levels of NtPOD, NtCAT, and NtCBF; were significantly elevated under abiotic stresses. We assumed that the resistance to abiotic stress in plants conferred by RmMYB44 was associated with the regulation of cell membrane integrity. This study aimed to elucidate the role of the RmMYB44 gene in the resistance mechanism of R. multiflora against abiotic stress, thereby providing a candidate gene for the molecular breeding of abiotic stress resistance in roses and related species.
{"title":"RmMYB44 Confers Resistance to Chilling, Drought, and Salt Stress in Both Rosa multiflora and Tobacco","authors":"Wuhua Zhang, Naiyu Zhang, Qi Qin, Xiaoying Zhang, Jinzhu Zhang, Tao Yang, Yifei Zhang, Jie Dong, Daidi Che","doi":"10.3390/agriculture14081212","DOIUrl":"https://doi.org/10.3390/agriculture14081212","url":null,"abstract":"Roses, a popular ornamental crop, often face various abiotic stresses during growth and development, such as cold, drought, and salinity. Rosa multiflora is a commonly used rootstock and exhibits strong resistance to both biotic and abiotic stresses, making it an ideal material for studying mechanisms for resistance. Among the largest plant families, MYB transcription factors play a crucial role in plant abiotic stresses. Our previous research has indicated that RmMYB44 could be involved in the low-temperature response of R. multiflora. This study further investigated RmMYB44, revealing that its expression levels were upregulated in response to chilling, drought, and salt stress. The results suggested its potential role as a key transcription factor in plant resistance to abiotic stresses. Additionally, RmMYB44 encoded a nuclear-localized protein without the self-activating function. The overexpression of RmMYB44 in tobacco plants enhanced the resistance to cold, drought, and salt stresses, as evidenced by the improved growth compared to wild-type (WT) plants under conditions of 4 °C, 30% water-holding capacity, and 200 mM of NaCl, respectively. Moreover, in overexpression tobacco plants, the levels of hydrogen peroxide and malondialdehyde (MDA) were significantly reduced; and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT); as well as the proline content and the expression levels of NtPOD, NtCAT, and NtCBF; were significantly elevated under abiotic stresses. We assumed that the resistance to abiotic stress in plants conferred by RmMYB44 was associated with the regulation of cell membrane integrity. This study aimed to elucidate the role of the RmMYB44 gene in the resistance mechanism of R. multiflora against abiotic stress, thereby providing a candidate gene for the molecular breeding of abiotic stress resistance in roses and related species.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"16 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141809235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alluvial fans have been proven to have great utilisation potential in QTP, but to what extent they are suitable for agricultural development is unknown. Therefore, using the alluvial fan in the Lhasa River Basin (LRB) as a case study, this paper established an evaluation system of land suitability for agriculture (LSA). Principal component analysis (PCA) and the exhaustive method (EM) were used to define the minimum dataset (MDS) and then the LSA of the alluvial fan in the LRB was evaluated using a comprehensive index of LSA. Two scientific approaches were implemented to improve the LSA using a scenario simulation. The results showed that the MDS assessed by the EA was more representative compared to the PCA. Alluvial fans with suitable grades are mainly distributed in the Lhasa River’s middle and lower reaches. Developing facility agriculture and repairing roads accessing the alluvial fans are effective approaches to improve the LSA, which can increase the suitable-grade area from 58.62% to 97.82% and 63.85%, respectively. Therefore, most alluvial fans in the LRB are suitable for developing agriculture, and under the influence of human activities, there will be more alluvial fans suitable for developing agriculture. Our research provides scientific methods for the sustainable development of land in alpine regions.
{"title":"Evaluation and Promotion of Alluvial Fan Land Suitability for Agriculture in the Lhasa River Basin, Qinghai–Tibet Plateau","authors":"Tongde Chen, Juying Jiao, Lingling Wang, Wei Wei, Chunjing Zhao, Shuwei Wei","doi":"10.3390/agriculture14081214","DOIUrl":"https://doi.org/10.3390/agriculture14081214","url":null,"abstract":"Alluvial fans have been proven to have great utilisation potential in QTP, but to what extent they are suitable for agricultural development is unknown. Therefore, using the alluvial fan in the Lhasa River Basin (LRB) as a case study, this paper established an evaluation system of land suitability for agriculture (LSA). Principal component analysis (PCA) and the exhaustive method (EM) were used to define the minimum dataset (MDS) and then the LSA of the alluvial fan in the LRB was evaluated using a comprehensive index of LSA. Two scientific approaches were implemented to improve the LSA using a scenario simulation. The results showed that the MDS assessed by the EA was more representative compared to the PCA. Alluvial fans with suitable grades are mainly distributed in the Lhasa River’s middle and lower reaches. Developing facility agriculture and repairing roads accessing the alluvial fans are effective approaches to improve the LSA, which can increase the suitable-grade area from 58.62% to 97.82% and 63.85%, respectively. Therefore, most alluvial fans in the LRB are suitable for developing agriculture, and under the influence of human activities, there will be more alluvial fans suitable for developing agriculture. Our research provides scientific methods for the sustainable development of land in alpine regions.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"90 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141807946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}