Pub Date : 2024-07-20DOI: 10.3390/agriculture14071194
Bo Chen, Na Yao
(1) Background: In the context of the Sustainable Development Goals (SDGs) and based on the historical evolution of cultivated land protection policy, we used the Smith model to address the problem of policy implementation bias and provide recommendations on how to ensure that cultivated land protection policy does not deviate in the implementation process. (2) Methods: The historical evolution process of China’s cultivated land protection policy was elucidated using literature analysis and the inductive deduction method; moreover, the issues regarding local evolution were investigated. In addition, utilizing the Smith model, which has an important role in policy implementation, the restoration direction of cultivated land protection in China was summarized. (3) Project: China’s cultivated land protection policy has demonstrated success in two stages of its historical development—from the basic stage of pursuing equal quantity and quality to the new stage of the trinity of “quantity–quality–ecology” of cultivated land. However, the trend of cultivated land protection policy development based on the regional division of labor in China still has some problems, i.e., the total quantity of cultivated land is insufficient, the quality is low, and the implementation of the trinity is unclear. These regional divisions depend on solving the balance of China’s grain security, and using the Smith model to solve the problem of policy implementation has become an important measure to resolve the issue of grain security and achieve the goal of cultivated land protection. (4) Conclusions: The Smith model is suitable for the implementation of China’s cultivated land protection policy. By utilizing the Smith model, the policy target group was found to be conducive to contributing to the maturity of and improvement in China’s cultivated land protection policy.
{"title":"Evolution Characteristics of Cultivated Land Protection Policy in China Based on Smith Policy Implementation","authors":"Bo Chen, Na Yao","doi":"10.3390/agriculture14071194","DOIUrl":"https://doi.org/10.3390/agriculture14071194","url":null,"abstract":"(1) Background: In the context of the Sustainable Development Goals (SDGs) and based on the historical evolution of cultivated land protection policy, we used the Smith model to address the problem of policy implementation bias and provide recommendations on how to ensure that cultivated land protection policy does not deviate in the implementation process. (2) Methods: The historical evolution process of China’s cultivated land protection policy was elucidated using literature analysis and the inductive deduction method; moreover, the issues regarding local evolution were investigated. In addition, utilizing the Smith model, which has an important role in policy implementation, the restoration direction of cultivated land protection in China was summarized. (3) Project: China’s cultivated land protection policy has demonstrated success in two stages of its historical development—from the basic stage of pursuing equal quantity and quality to the new stage of the trinity of “quantity–quality–ecology” of cultivated land. However, the trend of cultivated land protection policy development based on the regional division of labor in China still has some problems, i.e., the total quantity of cultivated land is insufficient, the quality is low, and the implementation of the trinity is unclear. These regional divisions depend on solving the balance of China’s grain security, and using the Smith model to solve the problem of policy implementation has become an important measure to resolve the issue of grain security and achieve the goal of cultivated land protection. (4) Conclusions: The Smith model is suitable for the implementation of China’s cultivated land protection policy. By utilizing the Smith model, the policy target group was found to be conducive to contributing to the maturity of and improvement in China’s cultivated land protection policy.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"114 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141820050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.3390/agriculture14071197
Sergio Valdelomar-Muñoz, E. Murgado-Armenteros
Growing consumer interest in caring for the environment has motivated the development of multiple studies focused on discovering this variable’s impact on purchasing behaviour. However, a major gap still exists between attitude and pro-environmental behaviour caused by the need for greater environmental awareness, among other things. Therefore, knowing the environmental issues that worry consumers of agrifood products is important. This work digs deeper into this issue by analysing these environmental concerns and examining differences between countries with different levels of environmental sensitivity. CAWI methodology has been used to conduct an online survey in four countries (Spain, Germany, the United Kingdom, and Denmark), with 3200 valid responses analysed using qualitative and quantitative techniques. The obtained results highlight the existence of 11 major environmental dimensions or issues for consumers of agri-food products. In addition, a correspondence analysis identifies two key factors, the first focused on a range of environmental problems of a more general nature and the second with greater interest focused on concern for biodiversity conservation. This study has implications for both the agri-food sector and European agricultural policy.
{"title":"Environmental Concerns of Agri-Food Product Consumers: Key Factors","authors":"Sergio Valdelomar-Muñoz, E. Murgado-Armenteros","doi":"10.3390/agriculture14071197","DOIUrl":"https://doi.org/10.3390/agriculture14071197","url":null,"abstract":"Growing consumer interest in caring for the environment has motivated the development of multiple studies focused on discovering this variable’s impact on purchasing behaviour. However, a major gap still exists between attitude and pro-environmental behaviour caused by the need for greater environmental awareness, among other things. Therefore, knowing the environmental issues that worry consumers of agrifood products is important. This work digs deeper into this issue by analysing these environmental concerns and examining differences between countries with different levels of environmental sensitivity. CAWI methodology has been used to conduct an online survey in four countries (Spain, Germany, the United Kingdom, and Denmark), with 3200 valid responses analysed using qualitative and quantitative techniques. The obtained results highlight the existence of 11 major environmental dimensions or issues for consumers of agri-food products. In addition, a correspondence analysis identifies two key factors, the first focused on a range of environmental problems of a more general nature and the second with greater interest focused on concern for biodiversity conservation. This study has implications for both the agri-food sector and European agricultural policy.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"27 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141819737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.3390/agriculture14071196
D. Lemić, M. A. Galešić, Mario Bjeliš, Helena Virić Gašparić
Citrus fruits, particularly mandarins, are highly valued globally for their nutritional benefits and versatile culinary uses. However, the challenge of post-harvest decay, primarily due to blue mold (Penicillium italicum) infections, results in significant food losses and necessitates effective preservation strategies. Traditional methods often rely on fungicides, raising concerns about chemical residues and environmental impact. This study investigates the efficacy of ozone as an alternative approach to controlling blue mold in mandarins. Various gaseous ozone treatments were tested, including single, double, and triple treatments, with durations ranging from 10 to 60 min and concentrations from 3.3 to 20 ppm. Additionally, ozonated water treatments were evaluated with concentrations of 2, 4, and 6 ppm. To simulate a realistic infestation scenario, mandarins were artificially infected with P. italicum spores before undergoing both gaseous ozone and ozonated water treatments. The storage conditions for the mandarins were meticulously controlled, maintaining a humidity level of 50–60% and a temperature range of 10–12 °C. Each fruit was analyzed, and the presence of P. italicum infection was determined two and three weeks after the ozonation. Results indicated that ozone treatments significantly reduced mold growth, with gaseous ozone demonstrating efficacy rates up to 97.5% and ozonated water treatments achieving preservation rates between 95% and 97%. These results underscore ozone’s potential as a safe, efficient, and sustainable alternative to conventional fungicides, offering promising solutions for extending the shelf life of mandarins. Further research is recommended to optimize ozone treatment parameters, assess long-term effects on fruit quality and nutritional content, and refine application techniques to harness ozone’s potential in citrus fruit preservation fully. This approach not only addresses food security challenges but also aligns with global efforts to reduce chemical inputs in agriculture and promote environmentally sustainable practices.
{"title":"Ozone Treatment as a Sustainable Alternative for Suppressing Blue Mold in Mandarins and Extending Shelf Life","authors":"D. Lemić, M. A. Galešić, Mario Bjeliš, Helena Virić Gašparić","doi":"10.3390/agriculture14071196","DOIUrl":"https://doi.org/10.3390/agriculture14071196","url":null,"abstract":"Citrus fruits, particularly mandarins, are highly valued globally for their nutritional benefits and versatile culinary uses. However, the challenge of post-harvest decay, primarily due to blue mold (Penicillium italicum) infections, results in significant food losses and necessitates effective preservation strategies. Traditional methods often rely on fungicides, raising concerns about chemical residues and environmental impact. This study investigates the efficacy of ozone as an alternative approach to controlling blue mold in mandarins. Various gaseous ozone treatments were tested, including single, double, and triple treatments, with durations ranging from 10 to 60 min and concentrations from 3.3 to 20 ppm. Additionally, ozonated water treatments were evaluated with concentrations of 2, 4, and 6 ppm. To simulate a realistic infestation scenario, mandarins were artificially infected with P. italicum spores before undergoing both gaseous ozone and ozonated water treatments. The storage conditions for the mandarins were meticulously controlled, maintaining a humidity level of 50–60% and a temperature range of 10–12 °C. Each fruit was analyzed, and the presence of P. italicum infection was determined two and three weeks after the ozonation. Results indicated that ozone treatments significantly reduced mold growth, with gaseous ozone demonstrating efficacy rates up to 97.5% and ozonated water treatments achieving preservation rates between 95% and 97%. These results underscore ozone’s potential as a safe, efficient, and sustainable alternative to conventional fungicides, offering promising solutions for extending the shelf life of mandarins. Further research is recommended to optimize ozone treatment parameters, assess long-term effects on fruit quality and nutritional content, and refine application techniques to harness ozone’s potential in citrus fruit preservation fully. This approach not only addresses food security challenges but also aligns with global efforts to reduce chemical inputs in agriculture and promote environmentally sustainable practices.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"25 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141819371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.3390/agriculture14071195
Weiwei Cheng, Yu Wang, Changchao Wang, Zhonghua Liu
The solar greenhouse is a significant agricultural facility in China. It enables the cultivation of crops during periods that do not coincide with the natural growing season, thus alleviating the pressure on the supply of fruits and vegetables during the winter months. The primary rationale behind the necessity of greenhouse cultivation lies in the fact that the temperature conditions conducive to optimal crop growth can be precisely replicated within this controlled environment. However, it is important to acknowledge that a distinct low-temperature area persists under the film during the overwintering period, with the precise delineation of its boundaries and distribution patterns remaining uncertain. In order to investigate the characteristics of the temperature distribution within the marginal region under the solar greenhouse film, experimental studies, CFD simulations, and LSTM prediction models were employed. The results of these studies indicate that, during the overwintering period, a low-temperature region was observed with approximately equal temperatures near the film membrane. The maximum horizontal distance from the south-side bottom corner was 6130 mm, while the minimum height from the ground was 600 mm. The lowest temperature in the low-temperature region was 4 °C, and the maximum observed temperature difference within the same period in different months was 1 °C. Additionally, a region of elevated temperatures was observed under the film. The lowest temperature in this region was 36.7 °C, and the highest temperature point was within the optimal range for crop growth. The CFD numerical simulation results were consistent with the actual observations, and the LSTM prediction model demonstrated high reliability. The findings of this study offer a theoretical foundation for the distribution of high and low temperatures in solar greenhouses. Furthermore, the developed prediction model provides the necessary buffer time for control, thus enhancing the efficiency of greenhouse cultivation.
{"title":"The Marginal Effect and LSTM Prediction Model under the Chinese Solar Greenhouse Film","authors":"Weiwei Cheng, Yu Wang, Changchao Wang, Zhonghua Liu","doi":"10.3390/agriculture14071195","DOIUrl":"https://doi.org/10.3390/agriculture14071195","url":null,"abstract":"The solar greenhouse is a significant agricultural facility in China. It enables the cultivation of crops during periods that do not coincide with the natural growing season, thus alleviating the pressure on the supply of fruits and vegetables during the winter months. The primary rationale behind the necessity of greenhouse cultivation lies in the fact that the temperature conditions conducive to optimal crop growth can be precisely replicated within this controlled environment. However, it is important to acknowledge that a distinct low-temperature area persists under the film during the overwintering period, with the precise delineation of its boundaries and distribution patterns remaining uncertain. In order to investigate the characteristics of the temperature distribution within the marginal region under the solar greenhouse film, experimental studies, CFD simulations, and LSTM prediction models were employed. The results of these studies indicate that, during the overwintering period, a low-temperature region was observed with approximately equal temperatures near the film membrane. The maximum horizontal distance from the south-side bottom corner was 6130 mm, while the minimum height from the ground was 600 mm. The lowest temperature in the low-temperature region was 4 °C, and the maximum observed temperature difference within the same period in different months was 1 °C. Additionally, a region of elevated temperatures was observed under the film. The lowest temperature in this region was 36.7 °C, and the highest temperature point was within the optimal range for crop growth. The CFD numerical simulation results were consistent with the actual observations, and the LSTM prediction model demonstrated high reliability. The findings of this study offer a theoretical foundation for the distribution of high and low temperatures in solar greenhouses. Furthermore, the developed prediction model provides the necessary buffer time for control, thus enhancing the efficiency of greenhouse cultivation.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"107 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141820569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Beef production is the primary contributor to greenhouse gas emissions in animal husbandry. But the carbon emission distribution, potential for carbon reduction, and pathways in the main links of the beef cattle industry chain are not yet clear. Balancing efficiency and fairness while taking tailored emission reduction paths according to local conditions is crucial in helping to achieve the low-carbon animal husbandry and “dual carbon” goals. This research adopts an industry chain perspective to assess four leading beef production regions’ carbon emissions, potential, and pathways in China from 2007 to 2021. We use life cycle assessment, the potential index which considers efficiency and fairness; the results are as follows: (1) The potential for carbon emission reduction in China’s beef industry showed an inverted U-shaped change trend from 2007 to 2021, and the average potential for carbon emission reduction in most provinces fluctuated around 0.500. (2) The main producing areas of China’s beef industry were divided into four areas of high efficiency and low fairness (Area A); high efficiency and high fairness (Area B); low efficiency and low fairness (Area C); and low efficiency and high fairness (Area D). (3) Differentiated emission reduction pathways were designed to reduce emissions, highlighting the need to implement region-specific carbon reduction policies.
{"title":"Potential and Pathways of Carbon Emission Reduction in China’s Beef Production from the Supply Chain Perspective","authors":"Lijun Zhang, Gaofei Yin, Zihao Wei, Wenchao Li, Cha Cui, Mingli Wang, Chen Zhao, Huifeng Zhao, Fengrui Xue","doi":"10.3390/agriculture14071190","DOIUrl":"https://doi.org/10.3390/agriculture14071190","url":null,"abstract":"Beef production is the primary contributor to greenhouse gas emissions in animal husbandry. But the carbon emission distribution, potential for carbon reduction, and pathways in the main links of the beef cattle industry chain are not yet clear. Balancing efficiency and fairness while taking tailored emission reduction paths according to local conditions is crucial in helping to achieve the low-carbon animal husbandry and “dual carbon” goals. This research adopts an industry chain perspective to assess four leading beef production regions’ carbon emissions, potential, and pathways in China from 2007 to 2021. We use life cycle assessment, the potential index which considers efficiency and fairness; the results are as follows: (1) The potential for carbon emission reduction in China’s beef industry showed an inverted U-shaped change trend from 2007 to 2021, and the average potential for carbon emission reduction in most provinces fluctuated around 0.500. (2) The main producing areas of China’s beef industry were divided into four areas of high efficiency and low fairness (Area A); high efficiency and high fairness (Area B); low efficiency and low fairness (Area C); and low efficiency and high fairness (Area D). (3) Differentiated emission reduction pathways were designed to reduce emissions, highlighting the need to implement region-specific carbon reduction policies.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"113 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141821295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-19DOI: 10.3390/agriculture14071187
Xiao Chen, Jianling Hu, Yan Gong, Qingxu Yu, Zhenwei Wang, Xiaozhong Deng, Xinguo Pang
China’s small-arched shed-building machinery mostly adopts manual pole casting and mechanical planting, which have low building efficiency and mechanization. Therefore, we designed an automatic feeding device for shed poles to realize automatic single separation, orderly conveyance and timely dropping of poles. Considering shed pole-pitching pass rate as the evaluation index for the regression model, we adopted a three-factor, three-level experimental design and established the speed of the reclaiming ring, height of the falling shed poles and reclaiming ring spacing as the main influencing factors, obtaining 23.94 r/min, 408.799 mm and 1350 mm, respectively in experiments with a trellis qualification rate of 95.36%. Design-Expert 13 was used to perform analysis of variance and determine the optimal parameter combinations. The average measured trellis qualification rate in tests with the bench adjusted and the optimal parameter combination was 94.23%, with 1.13% relative error between test and theoretical optimization values. This confirmed the optimal parameter combination’s dependability. In field verification test results, pick-up card ring speed was 24 r/min; height of trellis pole drop, 410 mm; pick-up card ring spacing, 1350 mm; and pitching rate, 95.37%, obtaining 0.01% error compared with theoretically optimized values. The prototype operational performance was stable and satisfied design requirements.
{"title":"Design and Test of Automatic Feeding Device for Shed Pole of Small-Arched Insertion Machine","authors":"Xiao Chen, Jianling Hu, Yan Gong, Qingxu Yu, Zhenwei Wang, Xiaozhong Deng, Xinguo Pang","doi":"10.3390/agriculture14071187","DOIUrl":"https://doi.org/10.3390/agriculture14071187","url":null,"abstract":"China’s small-arched shed-building machinery mostly adopts manual pole casting and mechanical planting, which have low building efficiency and mechanization. Therefore, we designed an automatic feeding device for shed poles to realize automatic single separation, orderly conveyance and timely dropping of poles. Considering shed pole-pitching pass rate as the evaluation index for the regression model, we adopted a three-factor, three-level experimental design and established the speed of the reclaiming ring, height of the falling shed poles and reclaiming ring spacing as the main influencing factors, obtaining 23.94 r/min, 408.799 mm and 1350 mm, respectively in experiments with a trellis qualification rate of 95.36%. Design-Expert 13 was used to perform analysis of variance and determine the optimal parameter combinations. The average measured trellis qualification rate in tests with the bench adjusted and the optimal parameter combination was 94.23%, with 1.13% relative error between test and theoretical optimization values. This confirmed the optimal parameter combination’s dependability. In field verification test results, pick-up card ring speed was 24 r/min; height of trellis pole drop, 410 mm; pick-up card ring spacing, 1350 mm; and pitching rate, 95.37%, obtaining 0.01% error compared with theoretically optimized values. The prototype operational performance was stable and satisfied design requirements.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"104 38","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141821682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-19DOI: 10.3390/agriculture14071192
José Ángel Cabrera, P. Carbonell, J. Salinas, Adrian Grau, A. Alonso, Francisca Hernández, J. J. Ruiz, S. García-Martínez
Tomato (Solanum lycopersicum L.) is a crop that is affected by more than a hundred viral species. De la pera is a local varietal type of tomato that is very popular in southeastern Spain. However, it is highly susceptible to several viruses, such as Tomato yellow leaf curl virus (TYLCV), which is considered one of the most important diseases of tomato crops and is a limiting factor for production in both outdoor and protected crops, making it difficult to eradicate. This study shows the effect of gene introgression on the performance of traditional lines of De la pera by combining two genes that offer tolerance to TYLCV, Ty-1 and ty-5, on some yield and quality traits and on the antioxidant capacity of tomato fruits. Two pear tomato breeding families, UMH175 and UMH220, were evaluated. Four lines from each of the families with all homozygous combinations of the Ty-1 and ty-5 genes were studied. The results showed that the introgression of the ty-5 allele produced a slight negative effect on yield, mean fruit weight, total soluble solids, and titratable acidity, in contrast to Ty-1, which produced a large negative effect. None of the introgressions showed a negative effect on the antioxidant compounds. ty-5 is a promising gene for use in breeding programs.
番茄（Solanum lycopersicum L.）是一种受百余种病毒影响的作物。De la pera 是一种当地番茄品种，在西班牙东南部非常受欢迎。然而，它极易感染多种病毒，如番茄黄叶卷曲病毒（TYLCV），该病毒被认为是番茄作物最重要的病害之一，是室外和保护地作物生产的限制因素，很难根除。本研究显示了基因导入对 De la pera 传统品系性能的影响，通过结合两个对 TYLCV 有耐受性的基因 Ty-1 和 ty-5，对番茄果实的一些产量和品质性状以及抗氧化能力产生了影响。对两个梨番茄育种家族 UMH175 和 UMH220 进行了评估。研究了每个家系中带有 Ty-1 和 ty-5 基因所有同源组合的四个品系。结果表明，ty-5 等位基因的导入对产量、平均果重、总可溶性固形物和可滴定酸度产生了轻微的负面影响，而 Ty-1 则产生了较大的负面影响。ty-5是一个有望用于育种计划的基因。
{"title":"Effect of Introgression of Ty-1 and ty-5 Genes on Productivity, Quality, and Antioxidant Compounds in De la Pera Tomato Breeding Lines","authors":"José Ángel Cabrera, P. Carbonell, J. Salinas, Adrian Grau, A. Alonso, Francisca Hernández, J. J. Ruiz, S. García-Martínez","doi":"10.3390/agriculture14071192","DOIUrl":"https://doi.org/10.3390/agriculture14071192","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is a crop that is affected by more than a hundred viral species. De la pera is a local varietal type of tomato that is very popular in southeastern Spain. However, it is highly susceptible to several viruses, such as Tomato yellow leaf curl virus (TYLCV), which is considered one of the most important diseases of tomato crops and is a limiting factor for production in both outdoor and protected crops, making it difficult to eradicate. This study shows the effect of gene introgression on the performance of traditional lines of De la pera by combining two genes that offer tolerance to TYLCV, Ty-1 and ty-5, on some yield and quality traits and on the antioxidant capacity of tomato fruits. Two pear tomato breeding families, UMH175 and UMH220, were evaluated. Four lines from each of the families with all homozygous combinations of the Ty-1 and ty-5 genes were studied. The results showed that the introgression of the ty-5 allele produced a slight negative effect on yield, mean fruit weight, total soluble solids, and titratable acidity, in contrast to Ty-1, which produced a large negative effect. None of the introgressions showed a negative effect on the antioxidant compounds. ty-5 is a promising gene for use in breeding programs.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":" 531","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141823672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-19DOI: 10.3390/agriculture14071193
Huafei Zhou, Min Chen, Baoyan Li, Haining Chen, Hongtao Wang, Shaoli Wang, Binghui Luan, Baoyou Liu
The purpose of this study is to discover and excavate more key factors and signaling pathways that regulate the formation intensity of biofilms and to fully reveal the possible models affecting biofilm formation. By using gene homologous recombination and bioinformatics technology, a MutL protein-directed deletion mutant strain was successfully constructed. The growth status of the mutant strain was observed, and it was confirmed that, except for the change in cell morphology, there were no significant differences in growth and reproduction between the mutant strain and the wild-type strain. By using the induced biofilm formation technique, the significant decrease in biofilm formation in the MutL mutant strain was successfully verified. The plate confrontation test confirmed that the inhibitory ability of the mutant strain against rice blast fungus was not significantly different from that of the WT strain. The colonization ability of the mutant strain on rice stems was tested, and it was confirmed that the colonization ability of the mutant strain was significantly lower than that of the WT strain. In terms of the prevention and control effect of rice blast disease, the mutant strain showed a significant decrease. By using transcriptomic big data, the gene and pathway expression differences between the mutant strain and the WT strain during biofilm formation were analyzed. The analysis revealed no significant correlation with the previously reported spo0A and tapA-sipW-tasA pathways. The key factor capB of the polyglutamic acid signaling pathway, which affects the formation of the biological model, was found to have a significant decrease in expression. A mechanical hypothesis was proposed: MutL may participate in regulating the formation intensity of Bacillus biofilms by regulating the formation of glutamic acid to polyglutamic acid.
{"title":"MutL Significantly Regulates the Formation of Biofilms in Bacillus amyloliquefaciens YT1","authors":"Huafei Zhou, Min Chen, Baoyan Li, Haining Chen, Hongtao Wang, Shaoli Wang, Binghui Luan, Baoyou Liu","doi":"10.3390/agriculture14071193","DOIUrl":"https://doi.org/10.3390/agriculture14071193","url":null,"abstract":"The purpose of this study is to discover and excavate more key factors and signaling pathways that regulate the formation intensity of biofilms and to fully reveal the possible models affecting biofilm formation. By using gene homologous recombination and bioinformatics technology, a MutL protein-directed deletion mutant strain was successfully constructed. The growth status of the mutant strain was observed, and it was confirmed that, except for the change in cell morphology, there were no significant differences in growth and reproduction between the mutant strain and the wild-type strain. By using the induced biofilm formation technique, the significant decrease in biofilm formation in the MutL mutant strain was successfully verified. The plate confrontation test confirmed that the inhibitory ability of the mutant strain against rice blast fungus was not significantly different from that of the WT strain. The colonization ability of the mutant strain on rice stems was tested, and it was confirmed that the colonization ability of the mutant strain was significantly lower than that of the WT strain. In terms of the prevention and control effect of rice blast disease, the mutant strain showed a significant decrease. By using transcriptomic big data, the gene and pathway expression differences between the mutant strain and the WT strain during biofilm formation were analyzed. The analysis revealed no significant correlation with the previously reported spo0A and tapA-sipW-tasA pathways. The key factor capB of the polyglutamic acid signaling pathway, which affects the formation of the biological model, was found to have a significant decrease in expression. A mechanical hypothesis was proposed: MutL may participate in regulating the formation intensity of Bacillus biofilms by regulating the formation of glutamic acid to polyglutamic acid.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"106 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141820615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aims to evaluate the biocontrol potential of Meyerozyma guilliermondii (CECT13190), an endophytic yeast, and its role in mitigating the adverse effects of abiotic and biotic stress in cucumber plants. The relevance of this study lies in addressing the threat of Fusarium wilt, a major fungal disease that impacts cucumber crop productivity, as well as the exacerbation of food scarcity caused by climate change-induced abiotic stress factors such as high temperatures and drought. The study was conducted in a greenhouse environment where Cucumis sativus seedlings were exposed to biotic (F. oxysporum inoculation) and abiotic stress conditions (heat and water deficit). The impact of M. guilliermondii on treated plants’ physiology, growth, development, and flowering was assessed. The study confirmed the biocontrol activity of M. guilliermondii against F. oxysporum and highlighted its positive effects as a plant growth promoter. It enhanced overall plant health, activated natural defense mechanisms against F. oxysporum, and alleviated the detrimental impacts of abiotic stress. Notably, M. guilliermondii also induced early flowering in cucumber plants. This research underscores the potential of M. guilliermondii as a biocontrol agent for managing Fusarium wilt, enhancing stress tolerance, promoting early flowering, and offering promising prospects for sustainable crop production amidst fungal diseases and climate change-induced stressors. The findings emphasize the importance of utilizing M. guilliermondii to improve cucumber crop productivity and address food scarcity challenges.
本研究旨在评估内生酵母Meyerozyma guilliermondii（CECT13190）的生物防治潜力及其在减轻黄瓜植物非生物和生物胁迫的不利影响方面的作用。这项研究的意义在于应对影响黄瓜作物产量的主要真菌病害镰刀菌枯萎病的威胁，以及气候变化引起的非生物胁迫因素（如高温和干旱）造成的粮食匮乏的加剧。研究在温室环境中进行，将黄瓜幼苗置于生物胁迫（接种 F. oxysporum）和非生物胁迫（高温和缺水）条件下。评估了 M. guilliermondii 对被处理植物的生理、生长、发育和开花的影响。研究证实了 M. guilliermondii 对 F. oxysporum 的生物控制活性，并强调了它作为植物生长促进剂的积极作用。它增强了植物的整体健康，激活了针对 F. oxysporum 的自然防御机制，并减轻了非生物胁迫的不利影响。值得注意的是，M. guilliermondii 还能诱导黄瓜植株提前开花。这项研究强调了M. guilliermondii作为一种生物控制剂在管理镰刀菌枯萎病、增强抗逆性、促进提早开花方面的潜力，并为真菌病害和气候变化引起的胁迫下的可持续作物生产提供了广阔的前景。这些发现强调了利用 M. guilliermondii 提高黄瓜作物产量和应对粮食短缺挑战的重要性。
{"title":"Biocontrol Potential and Mitigation of Abiotic Stress Effects of Meyerozyma guilliermondii on Cucumber (Cucumis sativus L.)","authors":"Arancha Gómez-Garay, Darío Bonaventura Roca-Campos, Sofía Irles Sánchez, Beatriz Pintos López","doi":"10.3390/agriculture14071189","DOIUrl":"https://doi.org/10.3390/agriculture14071189","url":null,"abstract":"This study aims to evaluate the biocontrol potential of Meyerozyma guilliermondii (CECT13190), an endophytic yeast, and its role in mitigating the adverse effects of abiotic and biotic stress in cucumber plants. The relevance of this study lies in addressing the threat of Fusarium wilt, a major fungal disease that impacts cucumber crop productivity, as well as the exacerbation of food scarcity caused by climate change-induced abiotic stress factors such as high temperatures and drought. The study was conducted in a greenhouse environment where Cucumis sativus seedlings were exposed to biotic (F. oxysporum inoculation) and abiotic stress conditions (heat and water deficit). The impact of M. guilliermondii on treated plants’ physiology, growth, development, and flowering was assessed. The study confirmed the biocontrol activity of M. guilliermondii against F. oxysporum and highlighted its positive effects as a plant growth promoter. It enhanced overall plant health, activated natural defense mechanisms against F. oxysporum, and alleviated the detrimental impacts of abiotic stress. Notably, M. guilliermondii also induced early flowering in cucumber plants. This research underscores the potential of M. guilliermondii as a biocontrol agent for managing Fusarium wilt, enhancing stress tolerance, promoting early flowering, and offering promising prospects for sustainable crop production amidst fungal diseases and climate change-induced stressors. The findings emphasize the importance of utilizing M. guilliermondii to improve cucumber crop productivity and address food scarcity challenges.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"124 34","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141820773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-19DOI: 10.3390/agriculture14071191
Salvatore Privitera, Emanuele Cerruto, G. Manetto, Sebastian Lupica, David Nuyttens, D. Dekeyser, I. Zwertvaegher, M. R. Furtado Júnior, Beatriz Costalonga Vargas
Spray droplet diameters play a key role in the field of liquid plant protection product (PPP) application technology. However, the availability of various measurement techniques, each with its unique operating principles for evaluating droplet size spectra, can lead to different interpretations of spray characteristics. Therefore, in this study, four measurement techniques—Liquid Immersion (LI), Laser Diffraction (LD), Phase Doppler Particle Analysis (PDPA), and Shadowgraphy (SG)—were utilized to evaluate the droplet size distribution of agricultural spray nozzles. Additionally, PDPA and SG were used to assess the average velocity of spray droplets. Experiments were conducted in three different laboratories with the main aim of comparing results obtained with various types of equipment utilized under ordinary practical conditions. Spraying tests were carried out using three flat fan nozzles and an air-induction flat fan nozzle. As a general trend, the lowest values for droplet diameters were measured using the Laser Diffraction technique, followed by Shadowgraphy. The PDPA technique provided the highest values for mean diameters (D10, D20, and D30) and the numeric median diameter (Dn0.5), whereas the Liquid Immersion method yielded the highest values for the Sauter mean diameter (D32) and volumetric diameters (Dv0.1, Dv0.5, and Dv0.9). Importantly, all measurement techniques were able to discriminate the four nozzles based on their Dv0.5 diameter. Average droplet velocities showed a similar pattern across the four nozzles with the PDPA and the SG measurement techniques. The differences in diameter values observed with the four measurement techniques underline the necessity of always including reference nozzles in spray quality assessments to base classifications on relative rather than absolute values.
{"title":"Comparison between Liquid Immersion, Laser Diffraction, PDPA, and Shadowgraphy in Assessing Droplet Size from Agricultural Nozzles","authors":"Salvatore Privitera, Emanuele Cerruto, G. Manetto, Sebastian Lupica, David Nuyttens, D. Dekeyser, I. Zwertvaegher, M. R. Furtado Júnior, Beatriz Costalonga Vargas","doi":"10.3390/agriculture14071191","DOIUrl":"https://doi.org/10.3390/agriculture14071191","url":null,"abstract":"Spray droplet diameters play a key role in the field of liquid plant protection product (PPP) application technology. However, the availability of various measurement techniques, each with its unique operating principles for evaluating droplet size spectra, can lead to different interpretations of spray characteristics. Therefore, in this study, four measurement techniques—Liquid Immersion (LI), Laser Diffraction (LD), Phase Doppler Particle Analysis (PDPA), and Shadowgraphy (SG)—were utilized to evaluate the droplet size distribution of agricultural spray nozzles. Additionally, PDPA and SG were used to assess the average velocity of spray droplets. Experiments were conducted in three different laboratories with the main aim of comparing results obtained with various types of equipment utilized under ordinary practical conditions. Spraying tests were carried out using three flat fan nozzles and an air-induction flat fan nozzle. As a general trend, the lowest values for droplet diameters were measured using the Laser Diffraction technique, followed by Shadowgraphy. The PDPA technique provided the highest values for mean diameters (D10, D20, and D30) and the numeric median diameter (Dn0.5), whereas the Liquid Immersion method yielded the highest values for the Sauter mean diameter (D32) and volumetric diameters (Dv0.1, Dv0.5, and Dv0.9). Importantly, all measurement techniques were able to discriminate the four nozzles based on their Dv0.5 diameter. Average droplet velocities showed a similar pattern across the four nozzles with the PDPA and the SG measurement techniques. The differences in diameter values observed with the four measurement techniques underline the necessity of always including reference nozzles in spray quality assessments to base classifications on relative rather than absolute values.","PeriodicalId":7447,"journal":{"name":"Agriculture","volume":"104 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141821571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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