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Effect of Diet and Dietary Patterns on the Progression of Multiple Sclerosis: A Review 饮食和饮食模式对多发性硬化症进展的影响:综述。
Pub Date : 2024-10-21 DOI: 10.2174/012772574X325602240910075218
Rabie Khattab, Arafat Goja, Welayah AlAmmar

The link between diet and the progression of Multiple Sclerosis (MS) is a topic of growing interest and investigation within the medical community. This review explored the mechanisms through which dietary interventions can impact the course of MS and shape the clinical outcomes and quality of life of individuals with the disease. By synthesizing current knowledge from clinical studies and observational research, the review aimed to provide insights into the role of diet in managing MS. A comprehensive literature search was conducted, focusing on the effect of diet and dietary patterns on the progression of MS. Key findings indicated that individuals with higher diet quality exhibit reduced disability levels and lower symptom severity, emphasizing the importance of maintaining a healthy diet and adopting a holistic, healthy lifestyle in managing MS. The review also delved into the potential impact of macronutrients, vitamins, and minerals on the progression of MS, highlighting the importance of adequate nutrient intake for optimal health outcomes. Additionally, the study explored the association between dietary intake variations and the severity of MS, suggesting that further investigation is needed to understand the potential implications of nutrient deficiencies in MS patients. Overall, the review serves as a valuable resource for healthcare professionals and individuals living with MS, providing evidence-based dietary approaches that may help optimize health outcomes and mitigate the burden of the disease. It also calls for future research directions in the critical area of dietary management of MS to enhance our understanding and improve patient care.

饮食与多发性硬化症(MS)的进展之间的联系是医学界日益关注和研究的一个课题。本综述探讨了饮食干预对多发性硬化症的病程、临床结果和患者生活质量的影响机制。通过综合临床研究和观察性研究的现有知识,该综述旨在提供饮食在多发性硬化症管理中的作用的洞察力。我们进行了全面的文献检索,重点关注饮食和饮食模式对多发性硬化症进展的影响。主要研究结果表明,饮食质量越高的人,残疾程度越低,症状严重程度越轻,这强调了保持健康饮食和采取全面健康的生活方式对控制多发性硬化症的重要性。综述还深入探讨了宏量营养素、维生素和矿物质对多发性硬化症进展的潜在影响,强调了充足的营养摄入对获得最佳健康结果的重要性。此外,该研究还探讨了膳食摄入量的变化与多发性硬化症严重程度之间的关联,表明需要进一步调查以了解营养素缺乏对多发性硬化症患者的潜在影响。总之,该综述为医疗保健专业人员和多发性硬化症患者提供了宝贵的资源,提供了以证据为基础的饮食方法,有助于优化健康结果和减轻疾病负担。它还呼吁未来在多发性硬化症饮食管理这一关键领域的研究方向,以增强我们的理解并改善患者护理。
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引用次数: 0
Impact of Ultra-Processed Foods on Food Sustainability: Exposure Assessment and Health Implications. 超加工食品对食品可持续性的影响:暴露评估与健康影响》。
Pub Date : 2024-10-14 DOI: 10.2174/012772574X327683240910063234
Muskan Chadha, Ratnakar Shukla, Rohit Kumar Tiwari, Dharmendra Kumar Dubey, Karuna Singh

Excessive consumption of ultra-processed food (UPF) is a global health concern as-sociated with an increased incidence of non-communicable diseases. UPFs are characterized by their extensive industrial processing and high content of additives, preservatives, chemicals, and artificial ingredients that are used to mask defects or inferior ingredients of end food products, presenting a multifaceted challenge to contemporary society. Exposure assessment showed that almost 50% to 30% of the energy consumption per day comes from UPFs. The more processed a food is, the less likely it is to be healthy and nutritious. UPFs affect human health by increasing the risk of obesity, heart disease, cancer, depression, diabetes, etc., as these foods are typically made up of a large number of preservatives, high content of fats, sodium, and sugars, and low contents of protein, fiber, and potassium. The ingredients required for the production of UPFs are commonly derived from intensive monoculture and livestock of raw foods. This, in turn, exerts imminent effects on agriculture, biodiversity, and the global food system by altering the bioavailability of nutrients within agricultural soil, thereby increasing the dependency on ferti-lizers and insecticides. Recent studies have shown that over-consumption of UPF has an effect on culinary traditions, loss of biodiversity, human well-being, and food system sustainability. Therefore, for better food system sustainability, the consumption of UPFs should be reduced, and we should move toward minimal processing technologies, preferably for seasonal and or-ganic local products. Based on available data, there is an urgent need to implement policies and regulations for the agro-food industry to include nutritional and environmental criteria. This review explains the different dimensions of UPFs in relation to health, nutrition, environment, current challenges, future innovations, and wider sustainability concerns.

过度消费超加工食品(UPF)是一个全球性的健康问题,因为这与非传染性疾病发病率的增加有关。超加工食品的特点是工业化加工程度高,添加剂、防腐剂、化学品和人工配料含量高,用于掩盖最终食品的缺陷或劣质成分,给当代社会带来了多方面的挑战。暴露评估显示,每天近 50%至 30%的能量消耗来自 UPF。食品加工得越多,其健康和营养的可能性就越小。UPFs 影响人类健康,增加肥胖、心脏病、癌症、抑郁症、糖尿病等疾病的风险,因为这些食品通常由大量防腐剂、高含量脂肪、钠和糖,以及低含量蛋白质、纤维和钾组成。生产 UPF 所需的原料通常来自集约化的单一种植和生鲜畜牧业。这反过来又对农业、生物多样性和全球粮食系统产生了迫在眉睫的影响,改变了农业土壤中营养物质的生物利用率,从而增加了对杀肥剂和杀虫剂的依赖。最近的研究表明,过度消费 UPF 会影响烹饪传统、生物多样性的丧失、人类福祉和粮食系统的可持续性。因此,为了提高粮食系统的可持续性,应减少对 UPF 的消费,我们应转向最小加工技术,最好是加工季节性产品和有机本地产品。根据现有数据,迫切需要对农业食品行业实施包括营养和环境标准在内的政策和法规。本综述从健康、营养、环境、当前挑战、未来创新以及更广泛的可持续发展问题等方面阐述了 UPFs 的不同层面。
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引用次数: 0
Growth Promotion and In vitro Seed Germination of Lycium barbarum L. (Red Goji) Using Different Types of Cytokinins. 使用不同类型的细胞分裂素促进枸杞(红枸杞)的生长和离体种子发芽。
Pub Date : 2024-10-08 DOI: 10.2174/012772574X313421240902052600
Ainun Fazdilah Azmi, Nurul Izzati Osman

Introduction: Lycium barbarum L., commonly known as red goji berry, is a widely recognized plant-based medicinal herb with nutritional and therapeutic properties. In this study, the effects of various cytokinins on the germination of L. barbarum seeds and the growth of seedlings were investigated under in vitro conditions.

Methods: The berries were first surface sterilized and dissected, and the seeds were then cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 1.0, and 1.5 mg/L) of 6-benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN) for 10 weeks at 25±2ºC with a photoperiod of 16 hours and a light intensity of 1000 lux. Upon observation after 10 weeks of culture, all cytokinin-treated cultures produced 100% seed germination as early as 7 days. KIN at 0.5 mg/L produced plantlets with the greatest height (8.40 ± 0.97 cm) with extensive rooting and the greatest total chlorophyll production. Besides, KIN at 1.5 mg/L resulted in the highest number of leaves per plantlet (6.90 ± 0.72), while 1.0 mg/L of TDZ led to the greatest biomass, i.e., fresh weight (FW) of 0.328 ± 0.05 gram and dry weight (DW) of 0.023 ± 0.003 gram.

Results: All cytokinins used in this experiment (BAP, TDZ, and KIN) promoted different in vitro growth promotion responses in L. barbarum. The effects of different types and concentrations of cytokinin on the height of plantlets, number of leaves per plantlet, fresh and dry weight, the extent of rooting, and the chlorophyll content were demonstrated to be statistically significant.

Conclusion: This study provides valuable insights into optimizing in vitro cultivation techniques for goji berry propagation, which could contribute to developing superior cultivars and increased production of this superfruit in the future. For future perspectives, extended research in elucidating the underlying mechanism associated with cytokinin supplementation is imperative to understanding the roles of cytokinins and optimizing their effects on plant growth promotion.

简介枸杞(Lycium barbarum L.),俗称红枸杞,是一种被广泛认可的植物药材,具有营养和治疗功效。本研究在离体条件下研究了各种细胞分裂素对枸杞种子萌发和幼苗生长的影响:方法:首先对浆果进行表面消毒并剖开,然后将种子放在添加了不同浓度(0.5、1.0 和 1.5 mg/L)的 6-苄基氨基嘌呤(BAP)、噻虫嗪(TDZ)和激肽原(KIN)的 Murashige and Skoog(MS)培养基上培养 10 周,温度为 25±2ºC,光周期为 16 小时,光照强度为 1000 lux。培养 10 周后观察发现,所有细胞分裂素处理的培养物均能在 7 天内产生 100% 的种子萌发率。0.5 毫克/升的 KIN 产生的小苗高度最高(8.40 ± 0.97 厘米),根系广泛,叶绿素总产量最高。此外,1.5 毫克/升的 KIN 能使每个小植株的叶片数最多(6.90 ± 0.72),而 1.0 毫克/升的 TDZ 能使小植株的生物量最大,即鲜重(FW)为 0.328 ± 0.05 克,干重(DW)为 0.023 ± 0.003 克:结果:本实验中使用的所有细胞分裂素(BAP、TDZ 和 KIN)都对 L. barbarum 的体外生长有不同的促进作用。不同类型和浓度的细胞分裂素对小植株高度、每片小植株叶片数、鲜重和干重、生根程度和叶绿素含量的影响均具有统计学意义:本研究为优化枸杞的体外栽培繁殖技术提供了有价值的见解,有助于今后开发优良品种和提高这种超级水果的产量。展望未来,要了解细胞分裂素的作用并优化其对植物生长的促进作用,就必须扩大研究范围,阐明细胞分裂素补充的内在机制。
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引用次数: 0
Current State of Scientific Knowledge on Curcumin Encapsulation and Applications. 姜黄素封装和应用的科学现状。
Pub Date : 2024-10-07 DOI: 10.2174/012772574X330008240827052241
Wissam Zam, Mohamed Reda Zahi, Mohamed El Hattab

The yellow pigment curcumin has long been used in traditional medicine for its anti-inflammatory, antibacterial and antioxidant activities. Over the past half-century, scien-tific investigations have shown that curcumin is endowed with additional health benefits be-cause it can modify key molecular targets associated with a number of pathologies, such as diabetes, cancer, and arthritis, in addition to cardiovascular, multiple sclerosis, Alzheimer's, and Crohn's diseases. However, this molecule has several disadvantages, such as low bioavail-ability and solubility, severe oxidative destruction, light sensitivity, fast systemic clearance and breakdown at alkaline pH levels. To address these drawbacks, several methods of micro-encapsulation employing a variety of shell materials have been investigated. These techniques contributed toward the increase of curcumin's solubility and stability against heat, light, oxy-gen, and an alkaline pH. The various shell materials and methods used to microencapsulate this chemical are the main topics of this review. The use of microencapsulated curcumin in food, medicine, and cosmetics is also discussed in more detail. Recent relevant research from the last few years has been given in this area, along with future difficulties.

姜黄素是一种黄色色素,因其具有消炎、抗菌和抗氧化作用而长期被用于传统医学。在过去的半个世纪里,科学研究表明姜黄素具有更多的健康益处,因为它可以改变与糖尿病、癌症、关节炎等多种病症相关的关键分子靶点,此外还能改变心血管疾病、多发性硬化症、阿尔茨海默氏症和克罗恩病等疾病的分子靶点。然而,这种分子也有一些缺点,如生物利用度和溶解度低、氧化破坏严重、对光敏感、全身清除快以及在碱性 pH 水平下分解。为了解决这些缺点,人们研究了几种采用各种外壳材料的微囊化方法。这些技术有助于提高姜黄素的溶解度和稳定性,使其不受热、光、氧和碱性 pH 值的影响。用于微囊化这种化学物质的各种外壳材料和方法是本综述的主要内容。此外,还详细讨论了微胶囊姜黄素在食品、医药和化妆品中的应用。此外,还介绍了过去几年在这一领域的最新研究成果以及未来的困难。
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引用次数: 0
Unveiling the Health Promising Benefits of Processed Foods Derived from Underutilized Edible Plants: A Review. 揭示未充分利用的食用植物加工食品对健康的益处:综述。
Pub Date : 2024-08-30 DOI: 10.2174/012772574X307460240722095039
Rafeeya Shams, Vinay Kumar Pandey, Divya Jain, Anjali Tripathi, Shivangi Srivastava, Gyanendra Tripathi

Wild food plants (WFPs) grow naturally and are essential components of the diet and conventional food chain. These plants are underutilized despite being nutritious and highly bio-active compounds. Wild food consumption has declined over the last generation owing to life-style changes and lower availability, and it is still consumed by marginalized communities. WFPs are available in a broad range of species and flavors that can help diversify the diet and make meals more enjoyable. Therefore, enhancing the availability and consumption of pro-cessed foods manufactured from wild plants is necessary. The increased use of processed prod-ucts formulated from edible wild plants can improve nutrition and protect ecological and cultural varieties. They are high in vitamins and micro-and macronutrients, which are essential for com-munities particularly vulnerable to malnutrition and imbalanced health. As plants develop natu-rally without the use of pesticides or other fertilizers, wild-food plants are typically considered more sustainable than commercial crops. Wild plants contain phytochemicals with various phar-macological and biological properties. Consuming WFPs should be done with caution and mod-eration, because some wild plants can be hazardous or harmful if consumed in large quantities or without adequate preparation. This review discusses various emerging technologies for han-dling wild food plants, the health benefits of these wild food plants, the effect of processing on reducing ant nutritional components, pharmaceutical potential, and consumers' overall percep-tions of wild food plants.

野生食用植物(WFP)自然生长,是饮食和传统食物链的重要组成部分。尽管这些植物营养丰富,具有很强的生物活性,但却未得到充分利用。由于生活方式的改变和可获得性的降低,野生食物的消费量在过去一代人的时间里有所下降,但边缘化社区仍然食用野生食物。世界粮食计划署提供的野生粮食种类繁多,口味各异,可以帮助人们实现饮食多样化,使膳食更加美味可口。因此,有必要增加野生植物加工食品的供应和消费。更多地使用以可食用野生植物为原料配制的加工食品可以改善营养状况,保护生态和文化多样性。它们富含维生素和微量及宏量营养素,对于特别容易营养不良和健康失衡的群体来说至关重要。由于植物是自然生长的,无需使用杀虫剂或其他肥料,因此野生植物通常被认为比经济作物更具可持续性。野生植物含有植物化学物质,具有各种植物学和生物学特性。食用野生植物应谨慎、适度,因为某些野生植物如果大量食用或未经充分准备,可能会对人体造成危害或伤害。本综述讨论了处理野生食用植物的各种新兴技术、这些野生食用植物对健康的益处、加工对减少蚂蚁营养成分的影响、制药潜力以及消费者对野生食用植物的总体看法。
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引用次数: 0
Buckwheat: An Underutilized Himalayan Crop with Multifaceted Nutraceutical Benefits. 荞麦:一种未被充分利用的喜马拉雅作物,具有多方面的营养保健功效。
Pub Date : 2024-08-27 DOI: 10.2174/012772574X323710240719073908
Kirti Raina, Ruchika Kumari, Palak Thakur, Rohit Sharma, Ashun Chaudhary

The human population is growing and alternate food options are needed to provide food and nutritional security to mankind. Reduced agricultural output as a result of climate change and increased demand for grains because of continuous population growth have created a gap between demand and supply of food. Buckwheat is a pseudocereal crop plant with high nutritional value that can be included as an alternate food in our diet. It is a traditional crop plant grown in the high mountains of the Himalayas for food as well as fodder. It completes its life cycle in 3-4 months, so is mostly grown as a second crop in between main crops like maize and barley. It also acts as a green manure by improving the phosphorus content of the soil. Buck-wheat has high nutritional value as it is rich in essential amino acids, vitamin B, trace elements, and other nutrients. The main bioactive compounds identified in buckwheat are rutin, quercetin, isoquercetin, d-chiroinositol, resveratol, and vitexin, which are responsible for its pharmacolog-ical properties. Research focused on value addition by exploring its nutritional, pharmaceutical, and other alternative uses of commercial importance, is needed for reviving buckwheat cultiva-tion practices and its conservation. Considering the multifarious applications of buckwheat, this review summarizes the currently available knowledge on the agronomic and nutraceutical sig-nificance of buckwheat to project its value as a future crop in the avenue of agriculture and functional food.

人类人口在不断增长,需要有其他的粮食选择来为人类提供粮食和营养保障。气候变化导致农业产量减少,而人口持续增长又增加了对谷物的需求,这就造成了粮食供需之间的缺口。荞麦是一种假谷类作物,具有很高的营养价值,可以作为我们饮食中的替代食物。荞麦是喜马拉雅山脉高山上种植的一种传统作物,既可作为食物,也可作为饲料。它的生命周期在 3-4 个月内完成,因此大多作为玉米和大麦等主要作物之间的第二茬作物种植。它还能改善土壤中的磷含量,起到绿肥的作用。荞麦富含人体必需的氨基酸、维生素 B、微量元素和其他营养物质,因此具有很高的营养价值。在荞麦中发现的主要生物活性化合物有芦丁、槲皮素、异槲皮素、d-螺肌醇、白藜芦醇和牡荆素,这些化合物是荞麦药理特性的来源。为了恢复荞麦的种植和保护,需要通过探索荞麦的营养、医药和其他具有重要商业价值的替代用途来开展增值研究。考虑到荞麦的多种用途,本综述总结了目前有关荞麦农艺学和营养学意义的知识,以预测其作为农业和功能食品领域未来作物的价值。
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引用次数: 0
Antioxidants for Skin Health. 抗氧化剂促进皮肤健康
Pub Date : 2024-08-06 DOI: 10.2174/012772574X311177240710100118
Virender Kumar, Neha Tanwar, Muskan Goel, Mohit Khan, Davinder Kumar, Gajendra Singh, Jyoti Mundlia, Naveen Khatri, Ashwani Kumar

Antioxidants play a pivotal role in maintaining skin health and integrity, combating the deleterious effects of oxidative stress induced by environmental aggressors such as UV ra-diation, pollution, and lifestyle factors. This paper reviews the contributions of key antioxidants, including Vitamin C, Vitamin E, Vitamin A, green tea extract, Coenzyme Q10, Resveratrol, Selenium, and Polyphenols, in skin health care. Vitamin C, known for its collagen synthesis promotion and photoprotection properties, alongside Vitamin E, a lipid-soluble antioxidant, syn-ergistically works to neutralize free radicals and repair damaged skin cells. Vitamin A, in the form of retinol, plays a critical role in skin cell regeneration and the maintenance of skin integ-rity. Green tea extract, rich in Polyphenols, offers anti-inflammatory and anticarcinogenic prop-erties, making it a potent ingredient for skin protection. Coenzyme Q10, a naturally occurring antioxidant in the body, aids in energy production for cell repair and regeneration, while Resveratrol, found in grapes and berries, provides anti-ageing benefits by enhancing skin's re-sistance to oxidative stress. Selenium, an essential mineral, contributes to the protection of skin cells from oxidative damage. The incorporation of these antioxidants in skincare products and dietary sources is discussed, highlighting the importance of a holistic approach in skincare re-gimes. The paper emphasizes the synergy between topical applications and dietary intake of antioxidants, advocating for a comprehensive strategy for promoting skin health and preventing age-related skin alterations. Method: For the review article, a variety of search engines and databases were used to identify relevant articles. Furthermore, for biomedical literature focusing on antioxidants and their ef-fects on skin health, PubMed was used. Moreover, to access a wide range of scholarly articles, including those related to dermatology and skincare, Google Scholar was used. Scopus provides comprehensive coverage of peer-reviewed literature across various scientific disciplines. Web of Science identifies high-impact articles and research on antioxidants in skincare. In addition, for accessing full-text articles on antioxidants and their applications in dermatology, Science Direct was used. The inclusion criteria for the review paper were as follows: only studies pub-lished in peer-reviewed journals were included to ensure the credibility and reliability of the information. Articles published in English were considered, to avoid language-related biases and ensure comprehension. Studies published within the last 10 years were included to provide the most current insights into antioxidant research in skincare. Articles must specifically focus on the role of antioxidants (Vitamin C, Vitamin E, Vitamin A, green tea extract, Coenzyme Q10, Resveratrol, Selenium, Polyphenols) in skin health care. Both experimental studies (in vivo and in vitro) and clinical

抗氧化剂在维护皮肤健康和完整性、对抗紫外线照射、污染和生活方式等环境因素引起的氧化应激的有害影响方面发挥着关键作用。本文回顾了维生素 C、维生素 E、维生素 A、绿茶提取物、辅酶 Q10、白藜芦醇、硒和多酚等主要抗氧化剂在皮肤保健方面的贡献。维生素 C 因其促进胶原蛋白合成和光保护特性而闻名,与维生素 E(一种脂溶性抗氧化剂)一起协同作用,中和自由基,修复受损的皮肤细胞。视黄醇形式的维生素 A 在皮肤细胞再生和保持皮肤完整性方面发挥着关键作用。绿茶萃取物富含茶多酚,具有抗炎和抗癌作用,是保护皮肤的有效成分。辅酶 Q10 是人体内天然存在的一种抗氧化剂,有助于细胞修复和再生的能量生产,而葡萄和浆果中的白藜芦醇则能增强皮肤对氧化压力的抵抗力,从而起到抗衰老的作用。硒是一种重要的矿物质,有助于保护皮肤细胞免受氧化损伤。本文讨论了将这些抗氧化剂纳入护肤产品和膳食来源的问题,强调了整体方法在护肤疗法中的重要性。本文强调了局部应用和膳食摄入抗氧化剂之间的协同作用,提倡采取综合策略促进皮肤健康和预防与年龄相关的皮肤变化。方法:为了撰写这篇综述文章,我们使用了多种搜索引擎和数据库来查找相关文章。此外,还使用了 PubMed 来检索有关抗氧化剂及其对皮肤健康影响的生物医学文献。此外,为了获取广泛的学术文章,包括与皮肤病学和护肤相关的文章,我们还使用了谷歌学术(Google Scholar)。Scopus 全面覆盖了各科学学科的同行评审文献。Web of Science 可查找有关护肤品中抗氧化剂的高影响力文章和研究。此外,为了获取有关抗氧化剂及其在皮肤病学中的应用的全文文章,还使用了 Science Direct。综述论文的纳入标准如下:只纳入在同行评审期刊上发表的研究,以确保信息的可信度和可靠性。考虑以英文发表的文章,以避免与语言相关的偏见并确保理解性。收录了过去 10 年内发表的研究报告,以便为护肤品中的抗氧化剂研究提供最新的见解。文章必须特别关注抗氧化剂(维生素 C、维生素 E、维生素 A、绿茶提取物、辅酶 Q10、白藜芦醇、硒、多酚)在皮肤保健中的作用。实验研究(体内和体外)和临床试验均包括在内,以提供有关抗氧化效果的全面概述。文章均为全文,以便进行全面的数据提取和分析。综述论文的排除标准如下:不包括未经同行审阅的出版物,如社论、观点文章和非学术性文章。由于潜在的翻译困难和保持一致性,以英语以外的语言发表的文章被排除在外。排除了不关注特定抗氧化剂或其对皮肤健康影响的研究。排除了重复发表的文章,以避免在综述中出现冗余。排除数据不充分或不完整的文章,以确保综述结果的质量和可靠性。
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引用次数: 0
Quantification using a HPLC-FLD-based Detector and Molecular Identifi-cation of Ochratoxin-A Producing Aspergillus Species Isolated from Stored Grain Samples. 利用基于高效液相色谱-荧光定量检测器对从储藏谷物样本中分离出的产赭曲霉毒素-A 的曲霉菌种进行定量和分子鉴定。
Pub Date : 2024-07-31 DOI: 10.2174/012772574X302254240610094258
Kanchan Gopal Choudhary, Choudhary Sharffuddin, Arun Kumar

Aim: Quantification using an HPLC-FLD based detector and Molecular identification of Ochratoxin-A producing Aspergillus Species isolated from stored grain samples.

Background: Fungi are cosmopolitan in origin and are known to grow in any suitable substra-tum. In the present investigation, Aspergillus species isolated from stored grain samples were analyzed for ochratoxin-A production.

Objective: The objective of this study is the quantification of Ochratoxin and identification of ochratoxigenic fungi.

Methods: A total of n=34 black Aspergilli and n=1 Ochre Aspergilli were isolated from grain samples of Bihar, India and it was tested for OTA production. The limit of detection (LOD) is found to be 0.33μg/Kg and the limit of quantification (LOQ) is found to be 1μg/Kg for OTA in HPLC-FLD.

Results: In the present study, out of all the fungal isolates, only TiB fungal isolate was able to produce the ochratoxin-A above the level of LOQ. The positive isolate TiB obtained from stored sesame seed samples was able to produce 25.54 μg/Kg of OTA. ITS sequence analysis of TiB isolate was able to matche 100% with Aspergillus welwitschiae and Aspergillus foetidus.

Conclusion: This is the initial report of ochratoxigenic Aspergillus fungal isolate isolated from stored sesame seed samples of flood-prone areas of Bihar, India.

目的:使用高效液相色谱-荧光定量检测器对从贮藏谷物样品中分离出的产生赭曲霉毒素-A 的曲霉菌进行定量和分子鉴定:背景:真菌起源于世界各地,可在任何适宜的底土中生长。本研究分析了从贮藏谷物样本中分离出来的曲霉菌种的赭曲霉毒素-A 产量:本研究的目的是定量检测赭曲霉毒素,并鉴定赭曲霉毒素致病真菌:方法:从印度比哈尔邦的谷物样本中分离出 n=34 个黑色曲霉菌和 n=1 个赭色曲霉菌,并检测其是否产生赭曲霉毒素。在 HPLC-FLD 中,OTA 的检出限(LOD)为 0.33 微克/千克,定量限(LOQ)为 1 微克/千克:本研究发现,在所有真菌分离物中,只有 TiB 真菌分离物产生的赭曲霉毒素-A 超过了定量限。从储存的芝麻样本中分离出的阳性分离物 TiB 能产生 25.54 微克/千克的 OTA。TiB 分离物的 ITS 序列分析结果与韦氏曲霉(Aspergillus welwitschiae)和福氏曲霉(Aspergillus foetidus)的 ITS 序列分析结果 100%吻合:这是首次报告从印度比哈尔邦易受洪水侵袭地区贮藏的芝麻样品中分离出的赭曲霉菌。
{"title":"Quantification using a HPLC-FLD-based Detector and Molecular Identifi-cation of Ochratoxin-A Producing Aspergillus Species Isolated from Stored Grain Samples.","authors":"Kanchan Gopal Choudhary, Choudhary Sharffuddin, Arun Kumar","doi":"10.2174/012772574X302254240610094258","DOIUrl":"https://doi.org/10.2174/012772574X302254240610094258","url":null,"abstract":"<p><strong>Aim: </strong>Quantification using an HPLC-FLD based detector and Molecular identification of Ochratoxin-A producing Aspergillus Species isolated from stored grain samples.</p><p><strong>Background: </strong>Fungi are cosmopolitan in origin and are known to grow in any suitable substra-tum. In the present investigation, Aspergillus species isolated from stored grain samples were analyzed for ochratoxin-A production.</p><p><strong>Objective: </strong>The objective of this study is the quantification of Ochratoxin and identification of ochratoxigenic fungi.</p><p><strong>Methods: </strong>A total of n=34 black Aspergilli and n=1 Ochre Aspergilli were isolated from grain samples of Bihar, India and it was tested for OTA production. The limit of detection (LOD) is found to be 0.33μg/Kg and the limit of quantification (LOQ) is found to be 1μg/Kg for OTA in HPLC-FLD.</p><p><strong>Results: </strong>In the present study, out of all the fungal isolates, only TiB fungal isolate was able to produce the ochratoxin-A above the level of LOQ. The positive isolate TiB obtained from stored sesame seed samples was able to produce 25.54 μg/Kg of OTA. ITS sequence analysis of TiB isolate was able to matche 100% with Aspergillus welwitschiae and Aspergillus foetidus.</p><p><strong>Conclusion: </strong>This is the initial report of ochratoxigenic Aspergillus fungal isolate isolated from stored sesame seed samples of flood-prone areas of Bihar, India.</p>","PeriodicalId":74644,"journal":{"name":"Recent advances in food, nutrition & agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141876917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cost-effective Phytohormone Extraction of Sargassum swartzii from the Persian Gulf Using Magnetic Ionic Liquid. 利用磁性离子液体从波斯湾马尾藻中提取具有成本效益的植物激素
Pub Date : 2024-07-26 DOI: 10.2174/012772574X315517240626065435
Ali Rajabiyan, Amanollah Zarei-Ahmady, Mohammad Izadi, Fatemeh Kardani

Introduction: Algae extracts are utilized as biofertilizers instead of chemical ferti-lizers in agriculture. Further, algae are known to possess a high content of plant hormones, such as gibberellin, salicylic acid, abscisic acid, and brassinosteroids.

Objective: The main objective of this study was to increase the extraction yield and simulta-neously extract hormones required for plant growth from Sargassum swartzii using Magnetic recoverable ionic liquid (IL).

Methods: In this study, extraction was performed by acidic digestion with acetic acid and then alkaline digestion with potassium hydroxide.

Results: The results showed the ionic liquid effect in extraction yield by 266 percent. The extracted phytohormones were analyzed using high-performance liquid chromatography (HPLC) methods. High levels of gibberellin, salicylic acid, abscisic acid, and brassinosteroids in improved algae extraction showed that seaweed extract could be used as environmentally friendly liquid bio-fertilizers to replace chemical fertilizers and could play a crucial role in organic farming for sustainable agriculture. Additionally, the recoverability of ionic liquid eight times with negligible leaching proved the introduced procedure to be cost-effective.

Conclusion: The reported procedure for algae extraction improved by using an acidic/primary ionic liquid environment. This procedure is economical because of the simple reusability of ionic liquid due to its magnetic features.

简介:在农业生产中,藻类提取物被用作生物肥料,而不是化学肥料。此外,众所周知藻类含有大量植物激素,如赤霉素、水杨酸、脱落酸和黄铜类固醇:本研究的主要目的是利用磁性可回收离子液体(IL)提高提取率,并同时提取马尾藻中植物生长所需的激素:方法:本研究先用醋酸进行酸性消解,然后用氢氧化钾进行碱性消解:结果表明,离子液体对萃取率的影响为 266%。提取的植物激素采用高效液相色谱法(HPLC)进行分析。改良海藻提取物中赤霉素、水杨酸、赤霉酸和铜固醇的含量较高,表明海藻提取物可用作环保型液体生物肥料,替代化肥,在有机农业的可持续发展中发挥重要作用。此外,离子液体的回收率为 8 倍,浸出率可忽略不计,这证明所引入的程序具有成本效益:所报告的藻类提取程序通过使用酸性/原离子液体环境得到了改进。由于离子液体的磁性特点,它可以简单地重复使用,因此该程序非常经济。
{"title":"Cost-effective Phytohormone Extraction of Sargassum swartzii from the Persian Gulf Using Magnetic Ionic Liquid.","authors":"Ali Rajabiyan, Amanollah Zarei-Ahmady, Mohammad Izadi, Fatemeh Kardani","doi":"10.2174/012772574X315517240626065435","DOIUrl":"https://doi.org/10.2174/012772574X315517240626065435","url":null,"abstract":"<p><strong>Introduction: </strong>Algae extracts are utilized as biofertilizers instead of chemical ferti-lizers in agriculture. Further, algae are known to possess a high content of plant hormones, such as gibberellin, salicylic acid, abscisic acid, and brassinosteroids.</p><p><strong>Objective: </strong>The main objective of this study was to increase the extraction yield and simulta-neously extract hormones required for plant growth from Sargassum swartzii using Magnetic recoverable ionic liquid (IL).</p><p><strong>Methods: </strong>In this study, extraction was performed by acidic digestion with acetic acid and then alkaline digestion with potassium hydroxide.</p><p><strong>Results: </strong>The results showed the ionic liquid effect in extraction yield by 266 percent. The extracted phytohormones were analyzed using high-performance liquid chromatography (HPLC) methods. High levels of gibberellin, salicylic acid, abscisic acid, and brassinosteroids in improved algae extraction showed that seaweed extract could be used as environmentally friendly liquid bio-fertilizers to replace chemical fertilizers and could play a crucial role in organic farming for sustainable agriculture. Additionally, the recoverability of ionic liquid eight times with negligible leaching proved the introduced procedure to be cost-effective.</p><p><strong>Conclusion: </strong>The reported procedure for algae extraction improved by using an acidic/primary ionic liquid environment. This procedure is economical because of the simple reusability of ionic liquid due to its magnetic features.</p>","PeriodicalId":74644,"journal":{"name":"Recent advances in food, nutrition & agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing Recent Technologies for Addressing Microplastic Pollution and Pushing the Case of Bioremediation as an Attractive Approach. 评估解决微塑料污染的最新技术,并将生物修复作为一种有吸引力的方法。
Pub Date : 2024-07-15 DOI: 10.2174/012772574X313370240620072831
Ekta Bhatt, Pammi Gauba, Indira P Sarethy

Microplastics are emerging sources of environmental pollutants that are increasingly of concern because of their harmful impacts on aquatic life and thereby humans. Their accumulation in the environment is in direct proportion to global plastic production; their being nondegradable, recalcitrant and of a persistent nature creates an urgent need to address this issue on a global scale. Recent reports have demonstrated the presence of microplastics in marine life, and directly becoming a part of the food chain when seafood is ingested by humans. The repercussions of these studies point to an even larger scale presence of microplastics across varied habitats, which are yet to be sampled. Bioremediation, using various microorganisms such as bacteria, algae and fungi, alone or as consortia or in biofilm form can be used as an effective remediation tool. Genetically modified microorganisms for focused removal of microplastics and metagenomics studies, providing taxonomic details of uncultured organisms, are also expected to provide an additional catalogue of technologies in this field. This review offers a comprehensive overview of microplastic sources, existing technologies for treating microplastics and an in-depth analysis of bioremediation mechanisms, its components, and the results from various studies which provide sufficient clues as to the directions to be chosen to address microplastics pollution and can facilitate and instruct researchers to further investigate the more practical approaches and create new and innovative strategies for advanced remediation of microplastic in the future.

微塑料是新出现的环境污染物来源,由于其对水生生物和人类的有害影响而日益受到关注。微塑料在环境中的累积量与全球塑料产量成正比;微塑料具有不可降解性、难降解性和持久性,因此迫切需要在全球范围内解决这一问题。最近的报告显示,海洋生物中存在微塑料,当人类摄入海产品时,微塑料直接成为食物链的一部分。这些研究的反响表明,微塑料在各种栖息地的存在规模更大,而这些栖息地尚有待采样。利用细菌、藻类和真菌等各种微生物,单独或以联合体或生物膜形式进行生物修复,可作为一种有效的修复工具。用于集中清除微塑料的转基因微生物和元基因组学研究(提供未培养生物的分类详情)也有望为该领域提供更多的技术目录。本综述全面概述了微塑料的来源、处理微塑料的现有技术,并深入分析了生物修复机制、其组成部分以及各种研究的结果,为解决微塑料污染问题提供了充分的线索,有助于并指导研究人员进一步研究更实用的方法,并在未来为先进的微塑料修复技术创造新的创新战略。
{"title":"Assessing Recent Technologies for Addressing Microplastic Pollution and Pushing the Case of Bioremediation as an Attractive Approach.","authors":"Ekta Bhatt, Pammi Gauba, Indira P Sarethy","doi":"10.2174/012772574X313370240620072831","DOIUrl":"https://doi.org/10.2174/012772574X313370240620072831","url":null,"abstract":"<p><p>Microplastics are emerging sources of environmental pollutants that are increasingly of concern because of their harmful impacts on aquatic life and thereby humans. Their accumulation in the environment is in direct proportion to global plastic production; their being nondegradable, recalcitrant and of a persistent nature creates an urgent need to address this issue on a global scale. Recent reports have demonstrated the presence of microplastics in marine life, and directly becoming a part of the food chain when seafood is ingested by humans. The repercussions of these studies point to an even larger scale presence of microplastics across varied habitats, which are yet to be sampled. Bioremediation, using various microorganisms such as bacteria, algae and fungi, alone or as consortia or in biofilm form can be used as an effective remediation tool. Genetically modified microorganisms for focused removal of microplastics and metagenomics studies, providing taxonomic details of uncultured organisms, are also expected to provide an additional catalogue of technologies in this field. This review offers a comprehensive overview of microplastic sources, existing technologies for treating microplastics and an in-depth analysis of bioremediation mechanisms, its components, and the results from various studies which provide sufficient clues as to the directions to be chosen to address microplastics pollution and can facilitate and instruct researchers to further investigate the more practical approaches and create new and innovative strategies for advanced remediation of microplastic in the future.</p>","PeriodicalId":74644,"journal":{"name":"Recent advances in food, nutrition & agriculture","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141636064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Recent advances in food, nutrition & agriculture
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