The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA)最新文献

Intentional replantation involves a combination of periodontics, endodontics, prosthodontics and oral surgery. Crown-root fracture management is still complicated nowadays. A fracture line extending longitudinally to the subgingival area and intruding bioogical width could affect infection control, gingival health and crown restoration. In the present study, we present two cases. Case 1 involved a 23-year-old man who presented at our hospital with crown-root fracture of the maxillary left central incisor. A radiographic image of the tooth revealed a fracture line under the alveolar crest. The fractured tooth was treated with intentional replantation with 180-degree rotation, root canal treatment and veneer restoration. The patient was followed up for 60 months. The replanted tooth functioned well, and no symptoms of resorption or ankylosis were observed by radiographic examination. Case 2 involved a 20-year-old woman who was referred to our hospital for crown-root fracture of her maxillary teeth. A radiographic examination revealed complicated crown-root fracture of the maxillary right lateral incisor and both maxillary central incisors. The central incisors were treated with intentional replantation with 180-degree rotation. At the 48-month follow-up, the fractured teeth were found to have regained normal function based on clinical and radiographic examination. Limited case reports are available on a long-term follow-up of intentional replantation with 180-degree rotation. These two cases, particularly case 2, presented optimal healing after 4 years with unideal crown-root ratios. This case report suggests that this old method of preserving teeth with crown-root fractures can be used as a last resort to save teeth owing to its timesaving and microinvasive procedure.

Objective: To investigate the efficacy of different obturation techniques on sealer penetration in teeth with internal root resorption using confocal laser microscopy.

Methods: An artificial internal resorption cavity (3 mm deep and 1.2 mm in diameter) was formed in the round-shaped root canals of 45 single-rooted teeth at a distance of 7 mm from the apex, then roots were instrumented (size 40/.06). The samples were divided into three groups (n = 15) according to the obturation technique: lateral compaction (LC), warm vertical compaction (WVC) and carrier-based (CB).

Results: In the resorption regions, the sealer penetration depth in the CB and LC groups was significantly higher than that in the WVC group (P < 0.05).

Conclusion: Within the limitations of this study, the penetration depth of the sealer in the resorption region was higher in the CB and LC groups as compared to that in the WVC group.

Objective: To explore whether hydrogen sulphide (H2S) could protect human periodontal ligament stem cells (PDLSCs) from senescence and the possible underlying mechanisms.

Methods: Cell cycle assay and Ki-67 assay were used to measure proliferation of PDLSCs. Real-time polymerase chain reaction (PCR) was used to measure cellular senescence-related p16 and p21. Calcium influx was detected by measurement of Ca2+ imaging. In addition, we analysed the possible mechanisms underlying H2S acting on PDLSCs by microarray.

Results: The cell proliferation rate of aging PDLSCs decreased significantly. The expression of cellular senescence-related p16 and p21 significantly increased in aging PDLSCs. H2S donor (GYY4137) treatment increased the proliferation rate of senescence PDLSCs. Furthermore, the donor of H2S treatment effectively prevented cell cycle arrest of PDLSCs during the aging process and inhibited the expression of cellular senescence-related markers. Mechanically, H2S donor treatment could activate the calcium influx in PDLSCs. Moreover, pretreatment with TRPV4 inhibitors significantly attenuated the calcium influx induced by H2S donor treatment in PDLSCs. It also alleviated the protective effect of H2S on the senescence of PDLSCs.

Conclusion: H2S alleviated the senescence of human PDLSCs by TRPV4 channel mediated calcium flux. These results provide a potential strategy to deal with cell aging and may facilitate cell therapy for oral diseases.

Objective: To evaluate the extension of canalis sinuosus (CS) into the alveolar crest for surgical reference in the anterior maxilla.

Methods: In this cross-sectional study, 485 CBCT images were evaluated in three orthogonal planes (axial, coronal and sagittal). The type of extension of CS into the alveolar ridge in the anterior maxilla was evaluated. The alveolar ridge was divided into four equal parts in a vertical and horizontal direction. In a vertical direction from apical to incisal and in a horizontal direction from labial to palatal, the four parts were designated as types 0, I, II and III, respectively. The extension of CS into the alveolar ridge was then traced.

Results: CS was present in 380 subjects (78.35%), and the extension type was unilateral in 217 of them (57.11%) and bilateral in 163 of them (42.89%). There was no significant relationship between incidence of CS and sex. Regarding the distribution of vertical and horizontal types, type II (the third quadrant of the ridge from apical to incisal and from labial to palatal, respectively) was significantly more prevalent than other types.

Conclusion: The most common location of CS into the alveolar ridge in both horizontal and vertical directions was type II (which is not close to the cortex). Awareness about the presence and possible locations of CS helps to reduce the risk of unjustifiable postoperative complications.

Objective: To analyse the correlation between self-perceived oral health (SPOH) and selfperceived general health (SPGH) and the influencing factors.

Methods: In this study, we collected sociodemographic information and details about SPOH and SPGH status for Chinese adults (≥ 18 years), and a total of 2233 people were included. The data were analysed using SPSS software (IBM, Armonk, NY, USA) with a chi-square test and binary logistic regression.

Results: In total, 43.4% of adults' self-perceived oral health was at a "good" level and 57.9% of adults' self-perceived general health was at a "good" level. The SPOH was correlated with SPGH (r = 0.593, P < 0.001). Good SPOH was associated with younger age, no dentures, no smoking, brushing teeth twice or more a day, periodontal health, no malocclusion and decayed, missing and filled teeth (DMFT) ≤ 12. Good SPGH was associated with younger age, higher educational level, no dentures, no smoking, brushing teeth twice or more a day, periodontal health, no malocclusion and DMFT ≤ 12.

Conclusion: SPOH and SPGH are correlated with each other, and greater attention should be paid to oral health to promote general health.

This clinical report describes the immediate implant placement and restoration for a 47-yearold woman with a protruded and loose maxillary right central incisor. The treatment included minimally invasive extraction, flapless immediate implant placement using a fully guided surgical template, and immediate implant-supported provisionalisation. The interim anatomical prosthesis was fabricated in advance based on preoperative CBCT scans, and the digital technique made it possible to integrate data precisely from different sources. After 6 months of provisionalisation, satisfactory gingival aesthetic and functional improvements were achieved, followed by a definitive screw-retained zirconia restoration. Thus, application of a complete digital workflow could reduce chairside time and create an optimal emergence profile that matches the residual bone architecture of the extraction sites with minimal interference.

Objective: To analyse the role played by Sappanone A, a bioactive ingredient isolated from the heartwood of Caesalpinia sappan, in the regulation of oral epithelial cell viability under radiation.

Methods: Cell viability of human oral keratinocytes (HOKs) and mouse salivary gland cells under ionising radiation was analysed. Expression of Ki67 was measured by immunohistochemical staining. Fragmentation of deoxyribonucleic acid (DNA) was measured by comet assay. Cell death was analysed using trypan blue exclusion assay. Cell viability was measured using a Cell Counting Kit 8 (CCK8; Abcam, Cambridge, UK) assay.

Results: Sappanone A decreased cell viability of HOK cells and mouse salivary gland cells under ionising radiation. In addition, Sappanone A enhanced radiation-induced genomic DNA fragmentation, accompanied by impaired homologous recombination and non-homologous end joining DNA repair. Mechanistic evaluation revealed that Sappanone A counteracted radiation-induced inosine monophosphate dehydrogenase 2 (IMPDH2) activation, and that this effect could be abolished by reconstituted expression of a Sappanone A-binding defective IMPDH2 mutant.

Conclusion: The present study highlights a novel role played by Sappanone A in the modulation of radiosensitivity of oral epithelial cells.

Objective: To screen miRNAs that could simultaneously regulate osteo/odontogenic differentiation of multiple stem cells, including dental pulp stem cells (DPSCs), stem cells from the apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs).

Methods: Differentially expressed miRNAs analysis on three miRNA microarrays data of dental stem cells undergoing osteo/odontogenic differentiation (GSE138180, GSE154466 and GSE159508) was performed, and miR-146a-5p were identified by bioinformatic prediction, dual-luciferase reporter assay and quantitative real-time polymerase chain reaction (PCR). In addition, differentially expressed genes between miR-146a-5p overexpressed group and control group (GSE79341) were applied for KEGG pathways enrichment analysis.

Results: MiR-146a-5p expression increased in the osteo/odontogenic differentiation of DPSCs, SCAPs and PDLSCs. Tumour necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) was identified as the target gene of miR-146a-5p. Furthermore, miR-146a-5p could influence the NF-Kappa B signalling pathway.

Conclusion: This study suggests that miR-146a-5p could promote differentiation in multiple dental stem cells through the NF-Kappa B signalling pathway by targeting TRAF6.

In this case study, we report the successful treatment of a 35-year-old woman with a hyperdivergent skeletal pattern, open bite and severe transverse deficiency, exhibiting a skeletal Class III malocclusion. The treatment plan included 3D correction of these issues with surgically assisted rapid maxillary expansion (SARME) to solve the transverse deficiency, presurgical orthodontic treatment including aligning and levelling of the teeth in both arches, LeFort I osteotomy and bilateral sagittal split ramus osteotomy, and postsurgical correction of malocclusion. Orthodontic treatment was performed with labial brackets, and the patient achieved satisfactory occlusion and a significantly improved facial profile. Retention at the 1-year follow-up showed stable occlusion and arch forms with a harmonious facial profile.