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Winter Barley Production in Relation to Crop Rotations, Fertilisation and Weather Conditions 冬大麦生产与轮作、施肥和天气条件的关系
Pub Date : 2018-04-01 DOI: 10.2478/agri-2018-0004
M. Babulicová, B. Dyulgerova
Abstract The aim of the study was to evaluate the influence of crop rotations, fertilisation and weather conditions on the grain yield and yield components of winter barley. Long-term field experiment was situated in the area of continental climate. There were the crop rotations with 40%, 60% and 80% share of the cereals. Two treatments of fertilisation were applied: H1 and H2. In the first treatment (H1), nitrogen, phosphorus and potassium in mineral fertilisers + organic manure Veget® were applied. In the second treatment (H2), nitrogen, phosphorus and potassium were applied in fertilisers only. Winter barley cultivar Barcelona was grown in years 2010-2015. The number of spikes per 1 m2, proportion of grains over sieve 2.5 + 2.8 mm and grain yield of winter barley were statistically significantly influenced by fertilisation. In the variant H1 (mineral fertilisation with addition of organic manure Veget®), the statistically higher (P ≤ 0.05) grain yield was obtained (5.71 t/ha) than in the treatment with mineral fertilisation only (5.30 t/ha). In the year 2011, which was very unfavourable for winter barley development, the grain yield in the treatment with mineral fertilisation with the addition of organic manure Veget® was by 1.23 t/ha (32.1%) higher than that in treatment with mineral fertilisa tion only.
摘要本研究旨在评价轮作、施肥和天气条件对冬大麦籽粒产量和产量组成的影响。长期野外试验设在大陆性气候区。有40%,60%和80%的谷物轮作。施H1和H2两种施肥处理。在第一处理(H1)中,施用矿物肥+有机肥中的氮、磷、钾。在第二处理(H2)中,只施用氮、磷和钾。冬大麦品种巴塞罗那种植于2010-2015年。施肥对冬大麦每m2穗数、过筛粒数2.5 + 2.8 mm和籽粒产量有显著的影响。在变异H1(添加有机肥Veget®的矿肥处理)中,籽粒产量(5.71 t/ha)显著高于单施矿肥处理(5.30 t/ha) (P≤0.05)。在对冬大麦生长非常不利的2011年,施用有机肥Veget®处理的籽粒产量比只施用有机肥处理的籽粒产量高出1.23吨/公顷(32.1%)。
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引用次数: 4
The In Silico Predicton of the Chloroplast Maturase K Gene Polymorphism in Several Barley Varieties 几种大麦品种叶绿体成熟酶K基因多态性的计算机预测
Pub Date : 2018-04-01 DOI: 10.2478/agri-2018-0001
K. Mustafa, Mufeed J. Ewadh, M. Al-Shuhaib, H. Hasan
Abstract This study was conducted to describe the role of the chloroplast maturase K (matK) genetic polymorphism in the reciprocal crossing between five barley varieties using several in vitro / in silico tools. Besides, the final consequences of the matK gene polymorphism on its protein structure, function, and interactions were predicted computationally. Five parental varieties were crossed to each other by full reciprocal crossing design, DNA was extracted from seeds and two different primers’ pairs were designed to scan matK gene. Then, polymerase chain reaction - single-stranded conformation polymorphism (PCR-SSCP) were performed. Two distinct haplotypes in both parents and artificial F1 hybrids in the matK gene were observed in both amplified fragments. This finding indicated that the studied gene had no participation in the reciprocal crossing performed. Three SNPs were identified; two of them are non-synonymous (nsSNPs), namely G387V and L459M. The effect of these missense mutations on the matK protein was analyzed by several in silico tools. It was shown that the coding SNP, L459M was predicted to have much more effective consequences on matK protein structure and function. While the I-Mutant 2.0 prediction tool showed a decrease in stability for these two nsSNPs, which may destabilize the protein interactions to some extent. In conclusion, though the observed missense mutations in the matK gene have no suggestive role in the reciprocally crossed barley varieties, they caused dramatic alterations in several matK protein moieties, which may lead to potential subsequent changes in the matK protein-mediated RNA splicing mechanisms.
摘要本研究利用体外/室内工具研究了叶绿体成熟酶K (matK)遗传多态性在5个大麦品种正交中的作用。此外,通过计算预测了matK基因多态性对其蛋白质结构、功能和相互作用的最终影响。采用全反交设计对5个亲本品种进行杂交,从种子中提取DNA,设计2对不同的引物扫描matK基因。然后进行聚合酶链反应-单链构象多态性(PCR-SSCP)分析。在两个扩增片段中均观察到双亲和人工F1杂交体中matK基因的两种不同的单倍型。这一发现表明所研究的基因没有参与反向杂交。鉴定出3个snp;其中两个非同义(nssnp),即G387V和L459M。这些错义突变对matK蛋白的影响通过几种硅工具进行了分析。结果表明,编码SNP L459M预计对matK蛋白的结构和功能有更有效的影响。而I-Mutant 2.0预测工具显示这两个nssnp的稳定性下降,这可能在一定程度上破坏了蛋白相互作用的稳定性。综上所述,虽然观察到的matK基因错义突变在相互杂交的大麦品种中没有提示作用,但它们引起了几个matK蛋白片段的剧烈变化,这可能导致matK蛋白介导的RNA剪接机制的潜在后续变化。
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引用次数: 9
The Effect of Different Tillage Methods on Erosion 不同耕作方式对土壤侵蚀的影响
Pub Date : 2018-04-01 DOI: 10.2478/agri-2018-0003
M. Vach, L. Hlisnikovský, M. Javůrek
Abstract During the years 2012‒2016 at the site threatened by erosion, the effect of different intensity and depth of soil tillage on the progression of erosion were evaluated on the plots with silage maize. Three different tillage methods were compared and evaluated - conventional tillage, including ploughing (CT), no-tillage using mulch and direct drilling (NT), and minimum tillage treatment with a lower depth of soil cultivation and organic matter incorporation (MT). Water and soil runoff on all of the experimental plots were measured during erosion events. Besides an analysis of naturally occurring rainfall causing erosions, we also conducted the test of soil infiltration abilities with a rain simulator after silage maize harvest. The effect of the tillage on aboveground biomass yield and the input costs was also analyzed. The results showed that NT and MT can significantly reduce water and soil runoff comparing CT. The highest yields were recorded in MT, while the lowest were in CT. Total input costs were higher in the case of NT and MT, but the share of mechanized work was lower for these technologies. Our results showed that NT and MT technologies, as a part of silage maize with a higher plant density stand establishment, should be a useable erosion control measure in areas vulnerable to erosion.
2012-2016年,以青贮玉米为研究对象,研究了不同耕作强度和深度对土壤侵蚀过程的影响。对三种不同的耕作方法进行了比较和评价:常规耕作,包括翻耕(CT),免耕覆盖和直钻(NT),以及低深度土壤耕作和有机质施肥(MT)的少耕处理。在侵蚀过程中测量了所有试验田的水和土壤径流量。除了分析自然降雨对土壤侵蚀的影响外,还对青贮玉米收获后的土壤入渗能力进行了模拟降雨试验。分析了耕作方式对地上生物量产量和投入成本的影响。结果表明:与CT相比,NT和MT能显著减少水土径流。旱作产量最高,旱作产量最低。在NT和MT的情况下,总投入成本较高,但这些技术的机械化工作份额较低。结果表明,NT和MT技术作为青贮玉米高密度林分的组成部分,在水土流失易发区应是一种有效的水土流失防治措施。
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引用次数: 3
Phenotyping Root System Architecture of Cotton (Gossypium barbadense L.) Grown Under Salinity 棉花根系结构的表型分析盐度下生长
Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0014
S. A. Mottaleb, E. Darwish, M. Mostafa, G. Safwat
Abstract Soil salinity causes an annual deep negative impact to the global agricultural economy. In this study, the effects of salinity on early seedling physiology of two Egyptian cotton (Gossypium barbadense L.) cultivars differing in their salinity tolerance were examined. Also the potential use of a low cost mini-rhizotron system to measure variation in root system architecture (RSA) traits existing in both cultivars was assessed. Salt tolerant cotton cultivar ‘Giza 90’ produced significantly higher root and shoot biomass, accumulated lower Na+/K+ ratio through a higher Na+ exclusion from both roots and leaves as well as synthesized higher proline contents compared to salt sensitive ‘Giza 45’ cultivar. Measuring RSA in mini-rhizotrons containing solid MS nutrient medium as substrate proved to be more precise and efficient than peat moss/sand mixture. We report superior values of main root growth rate, total root system size, main root length, higher number of lateral roots and average lateral root length in ‘Giza 90’ under salinity. Higher lateral root density and length together with higher root tissue tolerance of Na+ ions in ‘Giza 90’ give it an advantage to be used as donor genotype for desirable root traits to other elite cultivars.
土壤盐渍化对全球农业经济产生了深刻的负面影响。以耐盐性不同的2个埃及棉品种为材料,研究了盐胁迫对其早期幼苗生理的影响。此外,还评估了利用低成本的微型根管系统测量两个品种根系结构(RSA)性状变异的潜力。耐盐棉花品种吉萨90根系和地上部生物量显著高于盐敏品种吉萨45,根系和叶片对Na+的排除使其积累的Na+/K+比显著降低,脯氨酸含量显著高于盐敏品种吉萨45。在以固体MS营养培养基为底物的微型根管中测量RSA比泥炭苔藓/沙子混合物更精确和有效。在盐度条件下,吉萨90的主根生长率、总根系大小、主根长、侧根数和平均侧根长均高于其他品种。吉萨90较高的侧根密度和根长以及对Na+离子较强的根组织耐受性使其具有作为其他优良品种所需根系性状的供体基因型的优势。
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引用次数: 4
The Effects of Foliar Feeding of Compatible Organic Solutes on Agronomic Traits of Safflower 叶面补施有机溶质对红花农艺性状的影响
Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0013
M. Janmohammadi, Farzaneh Asadi, N. Sabaghnia, A. Abbasi, M. Nouraein, F. Shekari
Abstract Safflower is originated from Iran and is tolerant against water deficit stress. However, in semi-arid Mediterranean climate terminal drought and heat stress adversely affect the safflower production. In order to investigate the influence of foliar application of proline (Pr) (10 and 20 mM) and glycinebetaine (GB) (2 and 4 mM) under well and deficit irrigation (37.23° N,46.16° E). Foliar spray of compatible organic solutes started from middle vegetative growth and continued till seed filling stage. Comparison of well irrigated and stress conditions revealed that severity of water deficit stress (SI) was 0.25. Evaluation of growth-related morphological characteristics such as plant height, leaf area, canopy spread and percent ground cover showed that they considerably reduced by water deficit stress. However, foliar application of compatible solutes could somewhat increase growth related parameters. Results showed that water deficit stress noticeably reduced the chlorophyll content, while foliar spray could alleviate the water deficit stress effects when compared with intact plant (non-sprayed plants). The beneficial effect of GB was more prominent than Pr, especially under deficit irrigation condition. Principal component analysis (PCA) indicated that the best performance under well irrigated condition was obtained by application of 4 mM GB while under deficit irrigation condition the best performance was recorded for plants treated with 2 and 4 mM GB and 20 mM Pr. Overall, results of current experiments showed that foliar spray with high concentration of GB may can significantly alleviate the adverse effects of water deficit stress.
红花原产于伊朗,耐水分亏缺胁迫。然而,在半干旱的地中海气候中,极端干旱和热胁迫对红花的生产产生不利影响。为了研究在37.23°N,46.16°E的井灌和亏缺灌溉条件下叶面喷施脯氨酸(Pr)(10和20 mM)和甜菜碱(GB)(2和4 mM)的影响,从营养生长中期开始叶面喷施相容有机溶质,直至灌种期。井灌条件与胁迫条件比较,水分亏缺胁迫(SI)严重程度为0.25。植物生长相关的形态特征,如株高、叶面积、冠层铺展和土地覆被百分比,在水分亏缺胁迫下显著降低。然而,叶面施用相容溶质可以在一定程度上提高生长相关参数。结果表明,水分亏缺胁迫显著降低了叶绿素含量,而叶面喷淋与未喷淋植株相比,可以缓解水分亏缺胁迫的影响。在亏缺灌溉条件下,GB的有益作用比Pr更显著。主成分分析(PCA)表明,在井灌条件下,施用4 mM GB的植株表现最佳,而在亏缺灌溉条件下,施用2、4 mM GB和20 mM Pr的植株表现最佳。综上所述,目前的试验结果表明,高浓度GB叶面喷施可以显著缓解水分亏缺胁迫的不利影响。
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引用次数: 1
Uptake and Translocation of Some Heavy Metals by Rice Crop (Oryza sativa) in Paddy Soils 水稻土壤中某些重金属的吸收和转运
Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0016
G. Rahimi, Z. Kolahchi, A. Charkhabi
Abstract Accumulation of heavy metals in edible crops is amongst major international concerns today. While consuming Lenjan variety of rice is very popular in Iran, limited evidence exists on its safety. Amid increasing public concern about the safety of locally grown and imported rice in the market, a field study was carried out to investigate uptake and translocation of Cd, Pb, Ni, and Zn by a local variety of rice crop (Oryza sativa) exposed to contaminated water. At harvest time and in paddy fields, 41 soil and plant samples were collected from four locations of Lenjan, central Iran; irrigated from Zayandeh Rood River. In the laboratory, different parts of the plant were milled, digested via acid digestion method, and then analysed for Cd, Pb, Ni and Zn using atomic absorption spectrophotometry. The results showed that average concentrations of Cd, Pb, Ni and Zn were 1.07, 17.22, 1.73 and 13.75 mg/kg in the plant’s stem; and 1.27, 12.32, 1.099 and 19.39 mg/kg in its grain, respectively. In general, both in the plant’s stem and grain, the Cd and Pb concentrations were much higher than the FAO/WHO standard and labelled as harmful for consumers. Moreover, among the studied heavy metals, Ni transported very weakly, while Cd and Zn conveyed most easily into the plant’s stem and grain. Of course, Pb was the least mobile metal. However, it had highly accumulated in the plant’s stem and grain.
重金属在食用作物中的积累是当今国际关注的主要问题之一。虽然食用Lenjan品种的大米在伊朗非常受欢迎,但关于其安全性的证据有限。在公众对市场上本地种植和进口大米的安全性日益关注的背景下,开展了一项实地研究,调查当地品种水稻(Oryza sativa)暴露于受污染的水中对Cd、Pb、Ni和Zn的吸收和转运。在收获季节和水田中,从伊朗中部Lenjan的四个地点收集了41个土壤和植物样本;从Zayandeh路河灌溉。在实验室中,将植物的不同部位磨碎,用酸消化法消化,然后用原子吸收分光光度法分析Cd, Pb, Ni和Zn。结果表明:茎中Cd、Pb、Ni和Zn的平均浓度分别为1.07、17.22、1.73和13.75 mg/kg;籽粒含量分别为1.27、12.32、1.099和19.39 mg/kg。总的来说,在植物茎和籽粒中,镉和铅的浓度都远远高于粮农组织/世界卫生组织的标准,并被标记为对消费者有害。此外,在所研究的重金属中,Ni的运输非常弱,而Cd和Zn最容易进入植物的茎和籽粒。当然,铅是最不容易移动的金属。然而,它在植物的茎和籽粒中高度积累。
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引用次数: 26
Soil Organic-Matter in Water-Stable Aggregates Under Different Soil-Management Practices 不同土壤管理方式下土壤水稳性团聚体有机质
Pub Date : 2017-12-01 DOI: 10.1515/agri-2017-0015
V. Šimanský, J. Horák, B. Clothier, N. Buchkina, D. Igaz
Abstract An experiment of different management practices in a commercial vineyard, which was established in 2006 in the locality of Nitra-Dražovce, Slovakia on Rendzic Leptosol, was used to evaluate the dynamics of soil organic-matter parameters during the years 2008–2015. The following treatments were established: 1. G (grass without fertilisation as control), 2. T (tillage), 3. T+FYM (tillage + farmyard manure), 4. G+NPK3 (grass + 3rd intensity of fertilisation for vineyards: it means 125 kg/ha N, 50 kg/ha P, 185 kg/ha K), and 5. G+NPK1 (grass + 1st intensity of fertilisation for vineyards: it means 100 kg/ha N, 30 kg/ha P, 120 kg/ha K). The results showed that the soil-management practices in the vineyard significantly influenced the soil organic carbon in water-stable aggregates (SOC in WSA). The content of SOC in WSAma increased on average in the following order: T < G < G+NPK1 < G+NPK3 < T+FYM. Intensive soil cultivation in the T treatment resulted in a statistically significant build-up of SOC in WSAma at an average rate of 1.33, 1.18, 0.97, 1.22 and 0.76 g/kg/y across the size fractions > 5 mm, 5‒3 mm, 2–1 mm, 1–0.5 mm and 0.5–0.25 mm, respectively. The content of non-labile carbon reflected the contents of SOC in WSA. The highest labile carbon (CL) in WSAma, as compared to others, was found in T+FYM. Overall, application of higher NPK doses resulted in higher content of CL in WSAma compared with the lower applications of NPK. On the other hand, lower applications of NPK to soil increased the content of CL in WSAmi, as compared to G+NPK3.
摘要/ Abstract摘要:2006年,在斯洛伐克Nitra-Dražovce地区的一个商业葡萄园进行了不同管理措施的试验,以评估2008-2015年土壤有机质参数的动态变化。建立了以下处理:1。G(不施肥的草作为对照);3. T(耕作);3 . T+FYM(耕作+农家肥);G+NPK3(草+葡萄园的第三次施肥强度:这意味着125公斤/公顷氮,50公斤/公顷磷,185公斤/公顷钾)和5。G+NPK1(草地+葡萄园第一次施肥强度:N = 100 kg/ hm2, P = 30 kg/ hm2, K = 120 kg/ hm2)。结果表明,葡萄园土壤管理措施显著影响了水稳性团聚体土壤有机碳(SOC)。WSAma中SOC含量的平均增加顺序为:T < G < G+NPK1 < G+NPK3 < T+FYM。在T处理下,土壤集约耕作导致WSAma土壤有机碳积累的平均速率分别为1.33、1.18、0.97、1.22和0.76 g/kg/y,在> ~ 5 mm、5 ~ 3 mm、2 ~ 1 mm、1 ~ 0.5 mm和0.5 ~ 0.25 mm。非挥发性碳的含量反映了土壤有机碳的含量。与其他品种相比,WSAma中挥发性碳(CL)最高的是T+FYM。总体而言,施用高剂量氮磷钾比施用低剂量氮磷钾能提高水稻植株CL含量。另一方面,与G+NPK3相比,低氮磷钾用量使WSAmi土壤CL含量增加。
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引用次数: 7
Variability of quantitative and qualitative traits of coloured winter wheat 彩色冬小麦数量性状和质量性状的变异
Pub Date : 2017-10-01 DOI: 10.1515/agri-2017-0010
A. Žofajová, M. Havrlentová, M. Ondrejovič, Maroš Juraška, B. Michalíková, Ľubomíra Deáková
Abstract The aim of research was to analyse winter wheat of different grain colour and to compare newly bred coloured genotypes from our breeding in grain yield and technological and nutritional quality. The set of seven purple, five blue and four yellow wheats of different origin, including seven newly bred genotypes from Vígľaš-Pstruša, was evaluated in the field experiments established by randomised complete block design in two replications in Piešťany, in the vegetations 2012/13 and 2013/14. In seven wheat varieties differing in grain colours (selected after two of each colour plus control red variety Ilona) anthocyanin composition was evaluated by HPLC analysis. Significant differences were between growing years and among colour groups in most analysed traits. Blue grain newly bred K 3575 699/3 showed the highest anthocyanin content (by 33.5% higher compared to blue grain registered variety Scorpion). However, blue grain genotypes showed negative agronomic traits combined with low number and grain weight per spike and high plant height. In new purple variety PS Karkulka, declared grain yield and its quality were confirmed and the highest mineral content (Fe, Zn, Cu, Mn) was found in selected set. Purple grain newly bred PS 5711 had lower anthocyanin content (by 17.7%), but in quality it was comparable to PS Karkulka. Varieties with yellow endosperm showed the highest number and weight of grains per spike, however it was significantly lower to Ilona. The breeding goal of coloured winter wheat is still to improve the grain yield as well as additional agronomics traits.
摘要本研究的目的是分析不同籽色的冬小麦,并比较我们选育的新籽色基因型在籽粒产量、技术品质和营养品质上的差异。选取7个不同产地的紫色小麦、5个蓝色小麦和4个黄色小麦,包括7个来自Vígľaš-Pstruša的新基因型,在Piešťany的2012/13和2013/14植被中,采用随机完全区组设计建立了2个重复的田间试验。采用高效液相色谱法测定了7个籽粒颜色不同的小麦品种(每种颜色各选2个,外加对照品种伊洛娜)的花色苷成分。在大多数被分析的性状中,不同生长年份和不同颜色组之间存在显著差异。蓝粒新品种K 3575 699/3花青素含量最高(比蓝粒注册品种蝎子高33.5%)。蓝粒基因型表现出穗数和粒重低、株高高的负性农艺性状。紫色新品种PS卡库尔卡(PS Karkulka)的籽粒产量和品质得到了确认,其中选育组的铁、锌、铜、锰矿物含量最高。紫色籽粒新品种PS 5711花青素含量较低(17.7%),但品质与PS Karkulka相当。胚乳黄色的品种每穗粒数和粒重最高,但显著低于伊洛娜。彩色冬小麦的育种目标仍然是提高籽粒产量和附加农艺性状。
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引用次数: 6
Impact of operating temperature of gas transit pipeline on soil quality and production potential of crops 输气管道运行温度对土壤质量和作物生产潜力的影响
Pub Date : 2017-10-01 DOI: 10.1515/agri-2017-0012
D. Halmová, Z. Poláková, L. Končeková, A. Fehér
Abstract The aim of this study is to investigate the effects of gas transit pipeline temperature on soil moisture, soil temperature and yield of harvest crops. The study area was located in the village Ivanka pri Nitre (Nitra District, Southwestern Slovakia). Soil type in the site is Orthic Brown Chernozem. Temperature of the transported gas increased the soil temperature in the range of 2.07°C to 3.4°C measured in a depth ranging from 250 mm to 350 mm above the gas lines. The temperature also reduced soil moisture by 1.27‒3.18 percentiles of weight. Yield of the winter wheat grown above the gas lines was higher by 9.40% in 2004 and by 13.06% in 2006. Yield of the sunflower grown above the gas lines was higher by 8.05% in 2005. In treatment 1, organic fertilisation in a dose of 50 t/ha affected the yield of the winter wheat above the gas pipeline and the yield increased by 13.95% in 2004.
摘要本研究旨在探讨输气管道温度对土壤湿度、土壤温度及收获作物产量的影响。研究区域位于斯洛伐克西南部Nitra区Ivanka pri Nitre村。场地土壤类型为北褐黑钙土。在输气管道上方250 mm ~ 350 mm深度范围内,输送气体的温度使土壤温度升高2.07℃~ 3.4℃。温度还使土壤湿度降低了1.27-3.18个百分位数。输气管线以上冬小麦产量2004年提高9.40%,2006年提高13.06%。2005年瓦斯管线以上的向日葵产量高出8.05%。在处理1中,施用50吨/公顷的有机肥影响了输气管道上方冬小麦的产量,2004年产量增加了13.95%。
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引用次数: 0
Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq “澳洲植物候选菌”和“Ca.”的分子检测。伊拉克的犬齿假体
Pub Date : 2017-10-01 DOI: 10.1515/agri-2017-0011
N. Alkuwaiti, T. A. Kareem, L. J. Sabier
Abstract The association of phytoplasma was investigated in symptomatic tomato (Solanum lycopersicum L.), eggplant (Solanum melongen L.), mallow (Malva spp.) and Bermuda grass (Cynodon dactylon L.) plants exhibiting witches’ broom and white leaf diseases, respectively. Total DNA was extracted from tomato (n=3), eggplant (n=2), mallow (n=2) and Bermuda grass (n=8) samples. Direct polymerase chain reaction (PCR) was performed using P1/P7 primer set, then PCR products were sequenced. Sequences obtained from tomato, eggplant and mallow shared 99% maximum nucleotide identity with phytoplasma belonging to subgroup 16SrII-D, and resulted therefore ‘Candidatus Phytoplasma australasia’-related. Sequences obtained from Bermuda grass showed 100% maximum nucleotide identity to 16SrXIV-A subgroup and were ‘Ca. P. cynodontis’-related. The study presents the first molecular confirmation and sequence data of presence of ‘Ca. P. australasia’ and ‘Ca. P. cynodontis’ in Iraq.
摘要以番茄(Solanum lycopersicum L.)、茄子(Solanum melongen L.)、锦葵(Malva spp.)和百达草(Cynodon dactylon L.)为研究对象,研究了植物原体在表现为“巫秆病”和“白叶病”的有症状植物中的关联。从番茄(n=3)、茄子(n=2)、锦葵(n=2)和百达草(n=8)样品中提取总DNA。采用P1/P7引物进行直接聚合酶链反应(PCR),并对PCR产物进行测序。从番茄、茄子和麦芽糖中获得的序列与属16SrII-D亚群的植物原体有99%的最大核苷酸同源性,因此与候选植物原体(Candidatus phytoplasma australasia)相关。从百慕达草中获得的序列与16SrXIV-A亚群的最大核苷酸同源性为100%,为Ca。p . cynodontis》有关。本研究首次证实了‘Ca’存在的分子和序列数据。P. australasia '和' Ca。伊拉克的犬齿假体。
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引用次数: 6
期刊
Agriculture (Pol'nohospodárstvo)
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