Zeitschrift fur mikroskopisch-anatomische Forschung最新文献

Pineal gland of the immature female pigs treated with progesterone and progesterone and estradiol simultaneously were investigated with light and electron microscopy. Both of the applied hormones influenced the structure of the pineal glands. Inhibitory effect of progesterone on the relative volume of mitochondria, granular vesicles and dense bodies of type one was observed. Administration of progesterone and estradiol simultaneously caused more complicated influence statistically significant increase of pinealocyte nuclei volume and at the same time significant decrease of mitochondria and granular vesicles.

Ultrastructure of the chromatoid body in rat spermatocytes and spermatids was studied by transmission electron microscopy. The following was found: 1. electron dense granules, 72.1 +/- 14.73 (SD) nm, that appeared to be both primary (assembling) and end (disassembling) structural subunits in the biogenesis of the chromatoid body, 2. relationship between chromatoid body and cytoplasmic microtubules, 3. ribbon-like structures and aggregates of 25 nm granules. The discussion focuses on a) a probable sequence of formation and breakdown of the chromatoid body, and b) the chromatoid body as an example of a common cellular design involving an interrelationship of dense material-smooth membranes-microtubules.

The quantitative aspects of the chick embryo colon organogenesis (proximal and distal tracts) have been studied from the 7th to the 15th day of incubation on histological sections by means of a computerized morphometric system. A semiautomatic digital system (Videoplan 2) was used. The area of total section, of the lumen, of the wall and its components (subserous stratum, muscle layer, lamina propria, epithelium) and the thickness of the epithelium and muscle layer were measured; the mean +/- S.E.M. of the obtained values was calculated. The percentage of shrinkage, due to histological procedures, was calculated for each day. The mean values for each day of incubation were modified on the basis of the shrinkage percentage. The differences between the mean values of the areas of the proximal and distal tract were statistically evaluated. Exponential curves and r coefficient were determined to evaluate the general growing pattern of the mean area of colonic wall components as a function of age. The main results demonstrate that in both tracts the component with the greatest growth was the muscle layer, while there are some differences between the proximal and distal tracts of the colonic anlage in the growing pattern of other wall components. The colonic anlage also shows a different developmental behaviour compared with that of the chick embryo ileum and duodenum.

Gossypol administered orally to male rats at a daily dose of 20 mg/kg body weight for 63 days caused hypertrophy of the cauda epididymal epithelium, with more than fourfold increase in height of the cells. The principal cells lost most of their microvilli and formed apical blebs which appeared to produce the dense secretory material which was found in the lumen. Less dramatic but similar changes also occurred after 9 days on the same regimen, with the height of the epithelium doubling. However after 19 days on this regimen, with the height of the epithelium doubling. However after 19 days on this regimen, the epithelium looked fairly normal apart from a maintained hypertrophy. As reported in other studies, the cauda epididymal sperm were severely damaged and immotile; many were decapitated and the oxygen uptake was low. Ultrastructural defects were abnormal or absent mitochondria, absence of plasma membranes and axonemal components and accessory fibres.

The endocrine cells in the stomach gland patch of the koala (Phascolarctos cinereus) were studied ultrastructurally. They were classified into 3 types based on the ultrastructural profiles of their endocrine granules and tentatively categorized as type I, II, and III endocrine cells. Type I cells contained round granules that were for the most part larger than those observed in the other 2 cell types. The granules ranged from moderate to relatively high in electron density. Type II cells were angular in shape and characterized by the presence of granules that were polymorphous in profile. Contents of the endocrine granules in type II cells also showed a range of high to moderate electron density. Type III cells were oval or pyramidal in shape. They contained highly polymorphous granules that were round, oval, dumbbell-like or comma in shape and characterized by the presence of a clear space or halo separating the high to low electron-dense core from the limiting membrane of granules. Type III cells were observed most often whereas type I and II cells were a less frequent observation.

2-(6-methoxy-2-naphthyl) propionic acid is introduced to treatment as non-steroid antiinflammatory drug (NSAID) under the trade-mark of Naprosyn. Inhibition of prostaglandin synthesis is regarded as the most likely mechanism of its action. In some patients, its side-effects include gastritis, reactivation of ulcerous niche, and upper gastrointestinal haemorrhage. The absence of complex studies addressed to the question of morphological and histochemical changes in gastric mucosa after oral administration of Naprosyn prompted our undertaking. In experimental animals, with varying doses (10 mg/kg and 50 mg/kg) and variously long administrations (1 week and 3 weeks), a trial has been reported here upon. In the frozen-sectioned preparations, the histochemical reaction for acid phosphatase activity, according to Gomori, was made. The paraffin sections were subjected to the HE staining, PAS staining according to McManus and with the Masson's method. Our results of the morphological and histochemical studies in rats support the clinical observations of mucosal destruction in stomach in patients after oral administration of Naprosyn.

It is known that epidermal Langerhans cells react with osmium-zinc iodide (ZIO) mixtures; therefore they can be visualized by this histochemical method. In the last few years it has been shown that Langerhans cells are closely related to the class of interdigitating cells (IDC) which are antigen presenting cells located in the T-dependent areas of lymph nodes and spleen. In this study the reactivity of murine IDC to ZIO has been assessed. Results demonstrate that ZIO procedure yields to a brilliant and selective staining of IDC. The reactivity pattern is quite similar to that previously observed in epidermal Langerhans cells. This finding gives further support to the concept that Langerhans cells and IDC are closely interrelated cell types.

The permeability of the meningeal blood vessels and cellular layers to horseradish peroxidase was studied 5, 10 and 15 minutes following intravasal or intraarachnoidal introduction of the marker. When applied intravasally, the horseradish peroxidase-containing solution easily passed through the walls of all meningeal vessels (dural, pial and the ones traversing the arachnoid space). The cells of the inner dural layer and dural neurotheliun delay the penetration of horseradish peroxidase into the cerebrospinal fluid-filled arachnoid space by 10 min--rats and 15 min--cats. The perivascular leptomeningeal cells and their processes restrict the passage of the marker into the arachnoid space in a similar way. These barrier functions of the leptomeningeal cells and the cells that comprise the interface zone between dura mater and the arachnoid are confirmed by experiments where the marker was injected into the arachnoid space.

The nasolacrimal duct of the newborn mouse was lined with the stratified cuboidal epithelium, while in the mouse after 15 days of age the epithelium was stratified squamous. Further, in the adult mouse the epithelium could not be classified definitely as typical stratified squamous epithelium, but was somewhat modified. It is suggested that the stratified cuboidal epithelium corresponds to the "intermediate epithelium (NAKANO 1986)", and transforms into the modified stratified squamous epithelium with passing through the typical stratified squamous epithelium.

The function of pituitary STH- and PRL-cells was studied under treatment of bromocriptine and haloperidol in 6-day old house cats. After histological staining, the intensity of the intracellular reaction was determined by a microphotometric technique. Additionally, the STH and PRL blood levels were measured by radioimmunoassays.