Pub Date : 2021-12-31DOI: 10.24252/al-kimia.v9i2.24660
Hesty Nuur Hanifah, Ginayanti Hadisoebroto
{"title":"Perbandingan Efektivitas Bioadsorben Berbagai Serbuk Kulit Buah Terhadap Logam Pb Dari Limbah Cair Laboratorium Farmasi","authors":"Hesty Nuur Hanifah, Ginayanti Hadisoebroto","doi":"10.24252/al-kimia.v9i2.24660","DOIUrl":"https://doi.org/10.24252/al-kimia.v9i2.24660","url":null,"abstract":"","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47718691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.24252/al-kimia.v9i2.23633
M. Taupik, Endah Nurrohwinta Djuwarno, Mohammad Adam Mustapa
{"title":"Kajian Fitokimia dan Identifikasi Senyawa Metaboli Sekunder Daun Pare (Momordica Charantia L.)","authors":"M. Taupik, Endah Nurrohwinta Djuwarno, Mohammad Adam Mustapa","doi":"10.24252/al-kimia.v9i2.23633","DOIUrl":"https://doi.org/10.24252/al-kimia.v9i2.23633","url":null,"abstract":"","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44838483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.24252/al-kimia.v9i2.23993
Muhsinun Muhsinun, Fena Prayunisa
{"title":"Kajian Kemampuan Adsorpsi Zeolit Alam Terimpregnasi Asam Lemak Hidroksamat sebagai Agen Pengkelat Ion Logam Tembaga","authors":"Muhsinun Muhsinun, Fena Prayunisa","doi":"10.24252/al-kimia.v9i2.23993","DOIUrl":"https://doi.org/10.24252/al-kimia.v9i2.23993","url":null,"abstract":"","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47276296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.24252/al-kimia.v8i1.11965
Novia Suryani, A. Fasya, R. Retnowati, A. Jannah
l-menthyl acetic is an ester with specific aroma that synthesized by reaction of l-menthol and acetic anhydride and useful in fragrance industry. This paper reports influenced variation of time reaction toward ester product and characteristics l- menthyl acetic based on TLC, FTIR, GC and GC-MS. Reaction using l-menthol, acetic anhydride and catalyst H2SO4 98 % in diethyl eter solvent at temperature 60 °C with variation of time reaction (45, 60, 75, 90, 105 minutes). The result of research is variation of time influence product with highest yield 88,43 % for 90 minutes. Monitoring TLC achived Rf = 0,82; analysis by FTIR achived specific wave number ester 1736,96 cm-1; analysis by GC achived tr = 14,82 minutes and analysis by GC-MS achived tr = 16,13 minutes with base peak m/z = 95.
{"title":"Synthesis Of L-Menthyl Acetic by Esterification L-Menthol and Acetic Anhydride with Variation Of Time","authors":"Novia Suryani, A. Fasya, R. Retnowati, A. Jannah","doi":"10.24252/al-kimia.v8i1.11965","DOIUrl":"https://doi.org/10.24252/al-kimia.v8i1.11965","url":null,"abstract":"l-menthyl acetic is an ester with specific aroma that synthesized by reaction of l-menthol and acetic anhydride and useful in fragrance industry. This paper reports influenced variation of time reaction toward ester product and characteristics l- menthyl acetic based on TLC, FTIR, GC and GC-MS. Reaction using l-menthol, acetic anhydride and catalyst H2SO4 98 % in diethyl eter solvent at temperature 60 °C with variation of time reaction (45, 60, 75, 90, 105 minutes). The result of research is variation of time influence product with highest yield 88,43 % for 90 minutes. Monitoring TLC achived Rf = 0,82; analysis by FTIR achived specific wave number ester 1736,96 cm-1; analysis by GC achived tr = 14,82 minutes and analysis by GC-MS achived tr = 16,13 minutes with base peak m/z = 95.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44653142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-29DOI: 10.24252/al-kimia.v8i1.10152
Haiyul Fadhli, Anita Lukman, R. Adawiyah
Antioxidant was a compound which possessed an activity as scavenger of free radicals. This study was aimed to examine the antioxidant activity of ethyl acetate fraction and isolated compound from stem barks of Bauhinia semibifida Roxb. Samples extracted by gradual maceration with n-hexane solvent, ethyl acetate and methanol respectively. Isolation was carried out using Vacuum Liquid Chromatrography and purification by column chromatography with Sephadex LH-20. Isolated compound (BS) obtained was ivory crystalline with melting point 163-165 0 C. The analysis of FT-IR spectra, UV spectra and the result of qualitative test by Liebermann Burchard reagent were is shown positive as a terpenoid. Antioxidant activity were evaluated by using DPPH method for ethyl acetate fraction and isolation compound in serial concentration of 1000; 500; 250; 125; 62,5; 31,25 ug/mL. The result found that ethyl acetate fraction (f4 and f11) of stem barks of Bauhinia semibifida showed antioxidant activity with IC 50 value of >1000 ug/mL; 179 ug/mL. In conclusion, F11 from ethyl acetate extract from stem barks of Bauhinia semibifida Roxb. exhibited an activity as scavenger of free radicals from DPPH. Keywords: A ntioxidant, Bauhinia semibifida Roxb , IC 50 , VLC
{"title":"Isolasi dan Uji Aktivitas Antioksidan Metabolit Sekunder dari Ekstrak Etil Asetat Kulit Batang Kangkang Katup (Bauhinia semibifida Roxb)","authors":"Haiyul Fadhli, Anita Lukman, R. Adawiyah","doi":"10.24252/al-kimia.v8i1.10152","DOIUrl":"https://doi.org/10.24252/al-kimia.v8i1.10152","url":null,"abstract":"Antioxidant was a compound which possessed an activity as scavenger of free radicals. This study was aimed to examine the antioxidant activity of ethyl acetate fraction and isolated compound from stem barks of Bauhinia semibifida Roxb. Samples extracted by gradual maceration with n-hexane solvent, ethyl acetate and methanol respectively. Isolation was carried out using Vacuum Liquid Chromatrography and purification by column chromatography with Sephadex LH-20. Isolated compound (BS) obtained was ivory crystalline with melting point 163-165 0 C. The analysis of FT-IR spectra, UV spectra and the result of qualitative test by Liebermann Burchard reagent were is shown positive as a terpenoid. Antioxidant activity were evaluated by using DPPH method for ethyl acetate fraction and isolation compound in serial concentration of 1000; 500; 250; 125; 62,5; 31,25 ug/mL. The result found that ethyl acetate fraction (f4 and f11) of stem barks of Bauhinia semibifida showed antioxidant activity with IC 50 value of >1000 ug/mL; 179 ug/mL. In conclusion, F11 from ethyl acetate extract from stem barks of Bauhinia semibifida Roxb. exhibited an activity as scavenger of free radicals from DPPH. Keywords: A ntioxidant, Bauhinia semibifida Roxb , IC 50 , VLC","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44676832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-19DOI: 10.24252/AL-KIMIA.V7I1.7940
Sjamsiah Sjamsiah, A. Arifuddin, Mashuri Masri, Sappewali Sappewali, I. Islamiah, Hardiyanti Hamrullah, Elmika Nesti
This study aims to analyze the physical and chemical properties of soil from paddy fields, garden lands and city area also determine the soil inhibitory test of dog saliva bacteria. The results showed that these three soil types have different physical chemical properties and have different inhibitory zones against dog saliva bacteria. The physical chemistry of soil from paddy fields, garden lands and city area (0 cm) are as follows: for color; dark chocolate; grayish brown; and blackish ash, for texture: clay; clay; and sandy clay, for moisture content: 7.14; 6.53; 2.13. for pH close to neutral around 6.52-7.03; Total N is low (0.08-0.23); organic C compounds are classified as moderate; nutrient content of Ca/Mg is classified as moderate (9.55 /1.70 - 14.89/2.22); cation exchange capacity is classified as high (25.32-29.03) in paddy fields and gardens and compounds. The inhibitory zone of the highest dog saliva bacteria is in garden soil (3.7 cm), then paddy soil (2.86 cm) and land in the city area (1.6 cm).
{"title":"Analisis Sifat Fisika Kimia Tanah dan Uji Daya Hambatnya terhadap Bakteri Air Liur AnjingLiar","authors":"Sjamsiah Sjamsiah, A. Arifuddin, Mashuri Masri, Sappewali Sappewali, I. Islamiah, Hardiyanti Hamrullah, Elmika Nesti","doi":"10.24252/AL-KIMIA.V7I1.7940","DOIUrl":"https://doi.org/10.24252/AL-KIMIA.V7I1.7940","url":null,"abstract":"This study aims to analyze the physical and chemical properties of soil from paddy fields, garden lands and city area also determine the soil inhibitory test of dog saliva bacteria. The results showed that these three soil types have different physical chemical properties and have different inhibitory zones against dog saliva bacteria. The physical chemistry of soil from paddy fields, garden lands and city area (0 cm) are as follows: for color; dark chocolate; grayish brown; and blackish ash, for texture: clay; clay; and sandy clay, for moisture content: 7.14; 6.53; 2.13. for pH close to neutral around 6.52-7.03; Total N is low (0.08-0.23); organic C compounds are classified as moderate; nutrient content of Ca/Mg is classified as moderate (9.55 /1.70 - 14.89/2.22); cation exchange capacity is classified as high (25.32-29.03) in paddy fields and gardens and compounds. The inhibitory zone of the highest dog saliva bacteria is in garden soil (3.7 cm), then paddy soil (2.86 cm) and land in the city area (1.6 cm). ","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44442324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-30DOI: 10.24252/al-kimia.v6i2.6668
Sjamsiah ,
The physicochemical propertis of forest honey (Apis dorsata) become one of the supporting qualities of honey. Good honey can be viewed from the quality of honey which refers to the Indonesian National Standard (SNI). Therefore, this research aims to determine the physicochemical properties of forest honey from the village of Bonto Manurung, Maros district, Tompobulu village, Pangkep district and Paranglompoa village, Gowa district in accordance with the SNI. The results showed that the best physicochemical properties of honey were from the village of Bonto Manurung, Gowa district which had a moisture content of 19.19 %b/b, ash content of 0.045 %b/b, viscosity of 14.82 poise , acidity is 42.43 mL N NaOH/kg, reducing sugar is 69 %b/b and HMF levels are 2.99 mg/kg. As for the analysis of metallic impurities, all samples examined forest honey is not contaminated by metals arsenic (As), cadmium (Cd) and lead (Pb).
{"title":"Penentuan Sifat Fisikokimia Madu Hutan (Apis dorsata) Asal Daerah Maros, Pangkep dan Gowa Propinsi Sulawesi Selatan","authors":"Sjamsiah ,","doi":"10.24252/al-kimia.v6i2.6668","DOIUrl":"https://doi.org/10.24252/al-kimia.v6i2.6668","url":null,"abstract":"The physicochemical propertis of forest honey (Apis dorsata) become one of the supporting qualities of honey. Good honey can be viewed from the quality of honey which refers to the Indonesian National Standard (SNI). Therefore, this research aims to determine the physicochemical properties of forest honey from the village of Bonto Manurung, Maros district, Tompobulu village, Pangkep district and Paranglompoa village, Gowa district in accordance with the SNI. The results showed that the best physicochemical properties of honey were from the village of Bonto Manurung, Gowa district which had a moisture content of 19.19 %b/b, ash content of 0.045 %b/b, viscosity of 14.82 poise , acidity is 42.43 mL N NaOH/kg, reducing sugar is 69 %b/b and HMF levels are 2.99 mg/kg. As for the analysis of metallic impurities, all samples examined forest honey is not contaminated by metals arsenic (As), cadmium (Cd) and lead (Pb).","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45564397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-26DOI: 10.24252/al-kimia.v6i2.5067
St. Chadijah, Hardiyanti Hardiyanti, Sappewali Sappewali
Hydroxyapatite (Hap) with molecular formula Ca5(PO4)3OH is calcium apatite bioceramics which can be found in human teeth and bones. This material is synthesized through a hydrothermal process with calcium precursors derived from fish bones yellow fin tuna (Thunnus albacores) and phosphate precursor NH4H2PO4 0.547 M. On research conducted long stirring time variation and its effect on rendemen and the resulting crystallites products. As for the variation of the long time of stirring used are 1, 2, 3, 4 and 5 hours. The optimum time of formation of hydroxyapatite obtained within 3 hours stirring from which content was 96.4156%. The material was analyzed by FTIR and XRD. Results showed their characterization by FTIR absorption carbonate groups (CO32-) at wave number 1400-2400 cm-1, a phosphate ion (PO43-) at wave number 500-1050 cm1- and hydroxyl ions (OH-) at wave number 3500 cm-1. It is from XRD analysis, that size and the shape of the crystal which was obtain within 2 hours of stirring are 41,9753 nm and hexagonal.
{"title":"ANALISIS HIDROKSIAPATIT DARI TULANG IKAN TUNA (THUNNUS ALBACORES) DENGAN XRF, FTIR, dan XRD","authors":"St. Chadijah, Hardiyanti Hardiyanti, Sappewali Sappewali","doi":"10.24252/al-kimia.v6i2.5067","DOIUrl":"https://doi.org/10.24252/al-kimia.v6i2.5067","url":null,"abstract":"Hydroxyapatite (Hap) with molecular formula Ca5(PO4)3OH is calcium apatite bioceramics which can be found in human teeth and bones. This material is synthesized through a hydrothermal process with calcium precursors derived from fish bones yellow fin tuna (Thunnus albacores) and phosphate precursor NH4H2PO4 0.547 M. On research conducted long stirring time variation and its effect on rendemen and the resulting crystallites products. As for the variation of the long time of stirring used are 1, 2, 3, 4 and 5 hours. The optimum time of formation of hydroxyapatite obtained within 3 hours stirring from which content was 96.4156%. The material was analyzed by FTIR and XRD. Results showed their characterization by FTIR absorption carbonate groups (CO32-) at wave number 1400-2400 cm-1, a phosphate ion (PO43-) at wave number 500-1050 cm1- and hydroxyl ions (OH-) at wave number 3500 cm-1. It is from XRD analysis, that size and the shape of the crystal which was obtain within 2 hours of stirring are 41,9753 nm and hexagonal.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47264683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.24252/al-kimia.v6i2.4996
Siti Jubaidah, Henny Nurhasnawati
The tabar kedayan (Aristolochia foveolata Merr) plant located in the Malinau district of East Kalimantan has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antiviral. One of the secondary metabolites in this plant is the flavonoids that can be used as antioxidants.The aim of this research are to analyze total flavonoid content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total flavonoid content using spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total flavonoid average on ethyl acetate fraction of 1,09%±0,03 then n-hexane fraction of 0,52%±0,05 and the smallest level of ethanol-water fraction of 0,40%±0,03.
{"title":"ANALISIS FLAVONOID TOTAL AKAR TABAR KEDAYAN (Aristolochia foveolata Merr)","authors":"Siti Jubaidah, Henny Nurhasnawati","doi":"10.24252/al-kimia.v6i2.4996","DOIUrl":"https://doi.org/10.24252/al-kimia.v6i2.4996","url":null,"abstract":"The tabar kedayan (Aristolochia foveolata Merr) plant located in the Malinau district of East Kalimantan has considerable biological active prospects as antioxidant, antibacterial, antiamuba, anti-inflammatory, antihepatotoxic and antiviral. One of the secondary metabolites in this plant is the flavonoids that can be used as antioxidants.The aim of this research are to analyze total flavonoid content of root tabar kedayan in fractionation with various nonpolar, semipolar and polar solvents. The analysis used in the determination of total flavonoid content using spectrophotometric method. Data of analysis used standard curve method based on absorbance data and concentration of standard solution. The results of this study obtained the highest total flavonoid average on ethyl acetate fraction of 1,09%±0,03 then n-hexane fraction of 0,52%±0,05 and the smallest level of ethanol-water fraction of 0,40%±0,03.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48213823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-24DOI: 10.24252/AL-KIMIA.V6I2.6263
Idar Idar, Shinta Kusumawardhani, Mia Tria Novianti
Salmonella typhi (S. typhi) infection is a zoonotic infection and known as salmonellosis. In the human body, salmonellosis causes an increase in high body temperature or known as typhoid fever that cause high morbidity and mortality in developing countries, especially Indonesia. It was found that out of 22 million cases of typhoid fever, 200 thousand of them ended in death. S. typhi often contaminates food that was consumed raw or not perfectly cooked, for example meat, eggs, dairy products, fruits and vegetables. The conventional method for detecting these bacteria is culture method which time consuming and need BSL 2 facilities. PCR was one of DNA based detection method that could overcome the culture method weakness. In this study conducted detection of Salmonella bacteria in raw vegetables which are usually consumed as fresh by using nested PCR method. The detection procedures were sample preparation; bacterial DNA isolation; amplification by using two sets of primer, ST1-ST2 in first round PCR and ST3-ST4 in second round PCR; and the characterization by using agarose electrophoresis. The results indicated that two of nine raw vegetables, tomatos and cabbages have been contaminated with Salmonella. We conclude that nested PCR could detect Salmonella contamination in raw vegetables.
{"title":"Deteksi Bakteri Patogen Salmonella typhi pada Sayuran yang dikonsumsi Mentah Menggunakan Metode nested Polymerase Chain Reaction","authors":"Idar Idar, Shinta Kusumawardhani, Mia Tria Novianti","doi":"10.24252/AL-KIMIA.V6I2.6263","DOIUrl":"https://doi.org/10.24252/AL-KIMIA.V6I2.6263","url":null,"abstract":"Salmonella typhi (S. typhi) infection is a zoonotic infection and known as salmonellosis. In the human body, salmonellosis causes an increase in high body temperature or known as typhoid fever that cause high morbidity and mortality in developing countries, especially Indonesia. It was found that out of 22 million cases of typhoid fever, 200 thousand of them ended in death. S. typhi often contaminates food that was consumed raw or not perfectly cooked, for example meat, eggs, dairy products, fruits and vegetables. The conventional method for detecting these bacteria is culture method which time consuming and need BSL 2 facilities. PCR was one of DNA based detection method that could overcome the culture method weakness. In this study conducted detection of Salmonella bacteria in raw vegetables which are usually consumed as fresh by using nested PCR method. The detection procedures were sample preparation; bacterial DNA isolation; amplification by using two sets of primer, ST1-ST2 in first round PCR and ST3-ST4 in second round PCR; and the characterization by using agarose electrophoresis. The results indicated that two of nine raw vegetables, tomatos and cabbages have been contaminated with Salmonella. We conclude that nested PCR could detect Salmonella contamination in raw vegetables.","PeriodicalId":7535,"journal":{"name":"Al-Kimia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48214468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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