Acta obstetrica et gynaecologica Japonica最新文献

In 10 years from January 1971 to January 1981, 145 cases with cystic teratoma were treated at Kurume University Hospital, and malignant degeneration was seen in 7 of them (4.8%). Their age distribution was from 43 to 78 years, and the mean age was 59 years; the distribution shifted to the older side. The malignant degeneration was squamous cell carcinoma in all of the cases, and in 1 case carcinoma in situ leads to invasive carcinoma was observed continuously to a normal epithelium; it was interesting histogenetically. In their prognoses 3 out of 7 cases died (1 case dying from decrepitude) but the rest 4 have been surviving for 4 to 10 years.

Occupied and unoccupied estradiol (E3) receptors were investigated by exchange assay in cytosol and nuclear extract of human endometrium. Cytosol E2 and progesterone (P) unoccupied receptors and nuclear occupied E2 receptor were measured throughout the menstrual cycle and in the tissues from patients administered oral contraceptives. Sedimentation profiles of these receptors were also studied; and following results were obtained: 1) No occupied E2 receptor was observed in cytosol and almost all of those were of unoccupied type. In nuclear extract, only 0-25% of receptor was of unoccupied type and the other receptor was of occupied type. 2) Highest binding activities of cytosol E2 and P receptors were found in late proliferative phase. Maximum binding sites (Bm) were 630 and 380 f mol/mg protein and dissociation constant (Kd) was 8.9 +/- 0.7 and 4.7 +/- 0.4 x 10(-10) M, respectively. In nuclear extract, binding peaks were observed in late proliferative and late secretory phase. Bm was 1.5 f mole/microgram DNA and Kd was 12.7 +/- 1.0 x 10(-10) M.

External tocodynamometric transducer loaded by 800 g weight on its back was placed on the abdomen of pregnant estimated by contraction woman and uterine contraction during NST and OCT was index which was defined by 10 minutes' total of amplitude (g/cm2) multiplied by the duration (min) at the half amplitude. Observations of OCTs were similar to intrapartum ones. OCT was more useful than NST in the prediction of fetal distress during labor. The adequate contraction index for OCT was 65 to 70 min . g/cm2.

We tried the central venous catheterization using the ECG monitoring lead by the catheter tip without the X-ray examination. The catheter consists of 5F sized, 70 cm, thin-walled polyethylene tube. The apex of the catheter is 60 degrees curved with heating and the exchangeable metal adaptor is attached in the proximal end. The catheter was introduced percutaneously into the cubital vein and filled with saline. The ECG lead from the catheter tip was recorded through the metal adaptor by the battery electrocardiograph. The introduction into the right atrium of the catheter was easily judged in almost all cases by the intraatrial ECG, namely, deep negative or biphasic P wave. We have tried this method in 72 cases and the success rate of insertion into the right atrium was as high as 87.5%. The right atrial pressure of the obstetrical group (19 term pregnant women) was significantly lower than that of the gynecological group (22 preoperative patients) (P less than 0.01). The pH of the right atrial blood in the obstetrical group was significantly higher than that in the gynecological group (P less than 0.01). PCO2, PO2, HCO3-, and the oxygen saturation of the obstetrical group were significantly lower than those of the gynecological group (P less than 0.01).

The effect of human chorionic gonadotropin (hCG) of different purities from various source (eg. normal pregnancy urine, normal chorionic tissue, hydatidiform mole and vesicular fluid of hydatidiform mole) on the transformation of peripheral human lymphocytes was studied. All kinds of crude preparations of hCG suppressed lymphocyte transformation in a dose-dependent manner, in the phytohemagglutinin-induced stimulation. In addition, purified products of hCG have been shown a more inhibitory capacity in vitro. The inhibitory activity, however, was found not to be correlated with the gonadotropic activity of the various preparations, whereas this experiment, implicating which was compared to the content of sialic acid (N-acetylneuraminic acid) of different preparations.

In the present experiments, human fetal kidneys obtained from two fetuses aborted at mid-term (gestational age 21 weeks) were perfused in vitro with labeled precursor including 14G-dehydroepiandrosterone (DHA) and 14C-dehydroepiandrosterone-sulfate (DHA-S). Radioactive metabolites extracted after the termination of perfusion from the perfusate and the tissue were separated the free fraction by ether and conjugate fraction by n-butanol. Furthermore, recovery of the radioactivities were separated and purified by thin layer chromatography. Radiochemical purity of the metabolites were achieved by recrystallization to constant specific activity. When DHA was used as a precursor, in the perfusate, DHA-S, delta 5-Androstenediol, delta 5-Androstenetriol were identified as the metabolites derived from DHA, and they were successively recrystallized. But, when DHA-S was used as a precursor, the radioactive purity of the metabolite was not obtained. The result of this perfusion studies demonstrate a powerful activity of steroids sulfokinase and a slight activity of steroids 16 alpha-hydroxylase, 17 beta-hydroxysteroid dehydrogenase in fetal kidney.

In order to determine whether or not collagenase activity of the uterine cervix can be influenced by estrone (E1), estradiol (E2), estriol (E3), equilin, dehydroepiandrosterone sulfate (DHA-S) or dehydroepiandrosterone (DHA), the collagenase activity of extract of connective tissue of the uterine cervix treated with the drug was measured. A higher collagenase activity was obtained in pregnant cervix than that in nonpregnant ones. There was no difference in collagenase activity of the uterine cervix extract between control and drug (5 X 10(-5) M of E1, E2, E3, equilin, DHA-S or DHA) groups both in nonpregnant and pregnant conditions. After preincubation of tissue with 1 X 10(-5) M of the said drug at 37 degrees C for 60 min, the enzyme activity of the supernatant was also investigated. In nonpregnant conditions, no increase in the enzyme activity was observed. On the contrary, significant increase of the enzyme activity was obtained in pregnant uterine cervix preincubated with equilin, E3, DHA-S or DHA comparing to that of control group. However, this increase was not observed by E1 or E2. Elastase activity was also investigated on cervical connective tissue both in nonpregnant and pregnant conditions. No increase in elastase activity was observed in the pregnant cervix comparing to that of nonpregnant group.

To investigate the relevancy of birth weight, data on 300 term newborn infants were subjected to a reevaluation of 31 variables related to body measurements of both the mother and child and to the placenta. Factor analysis was introduced to analyze the data obtained. Each group was composed of multiple factor axes, of which factor structures were rather simple in Appropriate for Date (AFD) for approximating the practical images, compared to Heavy for Date (HFD) and Light for Date (LFD). In AFD, the birth weight was thought contributive by the limiting effect of the placenta, independently of the measurements of the mother. We found that the birth weight in itself seems to have a different meaning in each group, and it is difficult to readily categorize the findings on the table of equivalent parameters for labelling of the newborn.