Angewandte Parasitologie最新文献

The occurrence of several tick species in selected areas of Eastern Germany is described. From May 1987 till December 1989 8,472 ticks from 430 places were examined. Ixodidae of the genus Ixodes, Dermacentor and Haemaphysalis as well as Argasidae of the genus Argas were identified. The most common species was Ixodes ricinus. Furthermore, an endemic area of Dermacentor reticulatus was detected in the Düben-Dahlen and Annaburg health. Two other species of ticks, which were found frequently and sometimes with a high intensity on the host were Ixodes hexagonus and Ixodes canisuga. On the other side the species Haemaphysalis concinna and Argas vespertilionis were present at only one respectively two places and with a low population density.

A total of 2,552 persons living in 9 villages along the Benue river valley, Mutum Biyu district of Gongola State, Nigeria were examined between October and December 1989 for filariasis. It is the first time a filariasis survey will be carried out in this state. 276 (10.8%) had Wuchereria bancrofti, 50 (2.0%) had Loa loa, 281 (11.0%) were positive for Mansonella perstans while 12 (0.5%) were positive for Onchocerca volvulus. Villages located near the Benue river had higher prevalence rates than those further away. Dermatitis and hydrocoele were common and clinical manifestations were associated with parasite types. Clinical symptoms without microfilaremia and microfilaremia without clinical symptoms were also observed.

It was investigated whether there is a linear correlation between the number of applied infective larvae of Ancylostoma caninum and the number of larvae excreted by bitches with the milk. The investigations were carried out with nine bitches that were infected percutaneously each with 5,000, 10,000 or 20,000 third stage larvae of Ancylostoma caninum at the day of conception. A clear linear correlation between the number of applied infective larvae of Ancylostoma caninum and the number of larvae excreted with the milk could be demonstrated only for the first week of lactation. Regarding the total investigated period of 28 days only a tendency towards such a correlation between infective dosage and excretion of larvae with the milk could be found.

The serological diagnosis of extraintestinal infections with E. histolytica by indirect immunofluorescence (IFAT) requires a corpuscular antigen. This is produced in our laboratory via polyxenic cultivation of E. histolytica strain HK 9 in a nutrient medium containing a simple salt mixture (Resembling the Ringer-mixture), calf serum and rice starch. It was the aim of the experiments described in this paper to search for a useful medium in which the amebae grow in high density but without substances disturbing the antigen production like debris or rice starch granules. It was shown that the cell culture media of EAGLE MEM and PARKER were easy to handle (contrary to the worldwide used DIAMOND medium TYI-S-33) and brought good results. After a period of 10 subcultures both media were suitable for the cultivation of E. histolytica for making an antigen reasonable for IFAT. As a side effect it was noticed that there was an adaptation phase during which the amebae grew slowly when the medium was changed to another which was completely different (from a cell culture medium to a Ringer-like solution).

Laboratory-derived females of Culex quinquefasciatus and Aedes aegypti were experimentally infected with Wuchereria bancrofti by allowing the insects to feed on infected volunteers of known microfilariae density between 24.00 and 2.00 hours. Of the 240 mosquitoes used for the experiments only 67 (55.8%) of C. quinquefasciatus and 83 (69.2%) of the Ae.aegypti actually fed on the volunteers' blood. After 16 days post-infection period, 50 (74.62%) of the engorged C. quinquefasciatus harboured infective L3 larval stages of W. bancrofti, while 16 (23.9%) and 1 (1.49%) harboured L2 and L1 pre-infective larval stages, respectively. On the other hand, no development to the L3 infective stage was observed in the engorged Ae.aegypti though 34 (40.96%) harboured L1 pre-infective stages of the parasite. Our results suggest that C.quinquefasciatus could be actively involved in the transmission of urban bancroftian filariasis in Nigeria.

The diagnostic potential of musculo-skeletal pain (MSP) in the rapid assessment of onchocerciasis of morbidity and of the impact of ivermectin treatment in a community is brought to picture. The clinical impression is that MSP is a common, early, chronic important symptom of onchocerciasis. With a significant association with onchocerciasis, MSP has the potential of being an important operational diagnostic clue in the detection of onchocerciasis. It has the advantage of being easily recognizable, and therefore can be applied by primary health care workers. MPS is proved to cause the greatest morbidity, particularly among working farmers in areas where the forest strain of O. volvulus is predominant. Thus, onchocerciasis is contributing more than was perhaps realized to the reduced output of work from farmers. However, MPS, a parasitic rheumatism, is proved to be eminently susceptible to treatment by DEC.

The quantitative uptake of Wuchereria bancrofti microfilariae by mosquito species of different susceptibility to filarial infection was studied. There was no relationship between the degree of susceptibility and the number of ingested microfilariae. However, in all tested mosquito strains the females ingested as a mean 1.2-1.9 times more microfilariae of W. bancrofti than expected.

The order of abundance of different mallophagan species on poultry birds in Dehradun (India) has been found to be Menopon gallinae greater than Menacanthus cornutus greater than Menacanthus stramineus greater than Goniocotes gallinae greater than Goniodes dissimilis greater than Lipeurus caponis greater than Lipeurus lawrensis tropicalis greater than Goniodes gigas. The intensity of these species upon 1249 birds has been recorded by coding system. The correlation between the monthly incidence of different species and environmental temperature as well as R.H. has been recorded. The degree of association between four most commonly occurring combinations has also been analysed.

In pond experiments the efficiency of different chemotherapy variants against swimbladder inflammation (SBI) was proved. Only by feeding of Fumagillin, specifically affecting Myxosporidians, the infection of kidney with Sphaerospora renicola as well as the force of SBI could be reduced. The chemotherapy with Metronidazole remained inefficacious, whereas the permanent feeding with Methylene blue prevented only the infection with Bothriocephalus acheilognathi and probably lowered insignificantly the breeding-losses on this way. Water-conditioning by means of chloride of lime only lowered the losses caused by gill necrosis, on SBI it had no influence. A relationship between gill necrosis and SBI, that seemed to be possible, could not been corroborated.