Archives de l'Institut Pasteur d'Algerie. Institut Pasteur d'Algerie最新文献

Serotyping of Listeria has contributed to bring to light on the origin of outbreaks listeriosis; on the other hand, it is also an additional expression for the identification of Listeria genus. In order to get better in this field, we have produced five factors sera: I, I/II, V/VI, VI, VII.

The efficacy of inhibitors (PEA, Ac Nal, Az de Na) in the isolation of strict anaerobes in polymicrobial sample. The efficacy of three inhibitors to select strict anaerobic bacteria in the polymicrobial sample had been studied. First step: The most frequent anaerobes encountered in the infections are isolated in the agar Columbia containing the different inhibitors. This step allowed us to check the inhibition of the germ we have to isolate. Next step: polymicrobial mixtures were made. The composition of which is very similar to the samples we receive in the laboratory. The swarming Proteus is the facultative anaerobic germ which gives us difficulties when isolating strict anaerobic bacteria. Then, the different mixtures were isolated separately in the agar in which the inhibitors were added. The plates containing Azide of Na and PEA gave us the best results.

171 samples have been taken from stools of sick persons in three departments of university and hospital centres of Algiers (C.H.U.A.): the department of maternity (C.H.U Parnet), the department of breast diseases (CPMC), the department of women's surgery (CPMC), 13 samples of C. difficile have been isolated with a frequency of 7.6 per 100 of cases. The rate of conveyance seems more important in adults in hospitals and often concerns sick persons enduring digestive cancer (10.25 per 100) or newborn with a frequency of 8.73 per 100.

The authors selected among 5 diagnosed cases of larva migrans. In this study, the authors reported a review of this disease.

Production of monoclonal antibodies against a wild strain of rabies virus. Cell fusion of SP 2/O, a murine myeloma against a wild strain of rabies virus has originated five monoclonal antibodies (M.A.) specific for virus nucleocapsid , one M.A. specific for virus glycoprotein and one M.A. specific for a viral membrane protein.

The Newcastle disease in Algeria: Study of pathogenic properties of the Newcastle disease viral strains isolated in Algeria. The study of pathogenic characters of 14 strains of Newcastle disease virus isolated from 1972 to 1982 was carried out: The following testS were realized: the mean lethal time for the chicken embryo, the pathogenic power index by intravenous way, the pathogenic power index by intracerebral way, the hemagglutination spectrum and the thermal stability of the hemagglutination. In short we can say that these isolated 14 strains are fast-growing.

Campylobacter jejuni has been researched in raw milk, skin and excrement of chicken. All milk samples are negatives but 66% of chicken skin and 12% of chicken excrements are positives. An enrichment phase using brucella broth added with horse blood and antibiotics is necessary.

We report the isolation of 10 strains of enterobacteriaceae with a new quinolone's resistance. This resistance was detected by a diminution of inhibition's diameter around the disk of pefloxacin , and confirmed by the determination of Minimal Inhibiting Concentrations (M.I.C.). These strains, moreover multiresistant to multiple antibiotics, were isolated from patient's urines with repetitive urinary tract infections (paraplegics, lithiasic patients). None of this patients was treated by new quinolones. The incorrect use of old quinolones is probably responsible for the selection of this new quinolone's resistance.

In this work, we reports results of bacteriologic analysis of 352 cooked meals (vegetables, meats, desserts). Although, 32% of analysed samples are bacteriologically good, 68% are contamined either by S.P.C but potentially dangerous either by pathogenic bacteria involved in food-born infections.

The titration of haemolytic complement in biological liquid cause inconvenient in calculating concentration, it was long and fastidious. We report a fast technic based on measure of hemolysis in microplate++ method, which exploitation of results, it does with an appropriate algorithm.