Bilten za hematologiju i transfuziju最新文献

Hemorrhagic tendencies are frequently seen in the neonatal period. From the practical point of view, recognition of physiologic deficiencies of coagulation factors and hemostasis is essential, for the differential diagnosis of hemorrhagic syndromes and adequate treatment. Values of coagulation factors in the newborn and comparison of our results and the results of other authors are presented. It seems that there are no essential changes in hemostasis of term infant, but many coagulation factors exhibit deficient clotting activity as like vitamin K-dependent factors (II, VII, IX, X). Values of fibrinogen, F. XI, XII, XIII are decreased. Those factors which physiologically low in the term infant reach normal adult levels in a few weeks, with the exception of factors IX and XI which may require several months to reach normal levels. In the same time authors presented the results of coagulation factors in mothers during delivery and term infants, to explain the nature of the disorders. For the investigation of these physiological defects, screening tests can be used.

Circulating immune complexes were demonstrated in about 50% sera of 70 patients with Hodgkin's disease. Circulating immune complexes were detected by using two methods. The inhibition of RF Latex agglutination detects those immune complexes which contain immunoglobulins G as antibodies. Another test applied in this work is based on the selective precipitation of soluble immune complexes by 3g% solution of polyethylene glycol (PEG). This test is suitable for further characterisation of immune complex constituents. Analyses of PEG precipitates obtained after adding PEG to sera of patients with Hodgkin's disease showed that immunoglobulin G were present in all analysed cases. In some analysed PEG precipitates beta-lipoproteins were also identified among precipitated proteins. The obtained results, therefore, suggest the possibility that detected complexes are not obviously immune complexes, but the serum protein complexes of some other type.

The recovery of erythroid committed percursor cells and pluripotent haemopoietic stem cells (CFU-S) was determined in polycythaemic mice treated with cyclophosphamide (Cy). Erythroid repopulating ability (ERA) of bone marrow cells, erythropoietin responsive cells (ERC) and CFU-S were estimated for 4, 8 and 7 days after treatment with 200 mg/kg b. w. Cy, respectively. The proliferation rate of CFU-S and erythroid repopulating cells (ERA cells) was followed at the same time on the basis of cytosine arabinoside suicide data. The results demonstrated the difference in CFU-S and ERA regeneration. ERA recovery preceded CFU-S regeneration at the time when ERC were severely depressed. The proliferation rate of CFU-S and ERA cells differed also during the regeneration after Cy. The results suggest contribution of erythroid committed cells that proceed ERC (preERC) in measurement of ERA in polycythaemic mice treated with Cy.

Agglutinins against rabbit erythrocytes (RaRBC) were detected in 60 investigated normal human ser in titres ranging 1:20 - 1:1280. Titres of saline anti-RaRBC were not dependent to temperature nor to the blood groups of donor sera. Saline agglutinins were identified as antibodies of IgM class based on the result of gelfiltration and absorptions on anion exchanger and SpA-Sepharose immunoadsorbent as well as on mercaptoethanol and sulfitolysis sensitivity. IgG anti-RaRBC antibodies incapable to agglutinate RaRBC in saline were detected using indirect Coombs test in the second protein peak of gelfiltration. Some characteristics of anti-RaRBC saline agglutinins were compared with human natural isohemagglutinins.

The aloimunisation of the pregnant woman has been showed as hard form Morbus Haemolyticus Neonatorum (MHN) Newborn twins. Prematurely born twins - monoegged and their mother have compatible blood group of ABO system (A). CC-fenotype exchange transfusion was given to one child while the other received blood of cc-fenotype later on, the both of them were given additional transfusion of CC-fenotype of corresponding blood group ABO. Mother was given CC-fenotype blood transfusion. Certain difference was seen in the fostness of restituciones of the value of hematocrit in the fostness of value normalizations of bilirubin and in the presence of anti c antibodies in red cells and in the sera of sick twins. The comparisons of the MHN course are limited by the fact that Newborn twins have neither of the same level of imperillness during the term of birth, nor of same fisiological characteristics. Six weeks after being born and after clinical treatment, the children were found health and to domestic treatment.

Haemoglobin F was determined in 19 patients with leukemia: 12 with LAM, 4 with LAL, 2 with LLC and 1 with LMC. Half of the patients with acute leukaemia was in remission and other half in evolution at the time of the study, later ones having anemia. Haemoglobin F was determined by the alcali resistance method. In 10 control cases haemoglobin F was 0.98 +/- 0.48%. Increased level of haemoglobin F was found in 7/12 LAM and 2/4 LAL patients. Eight of those 9 patients with increased haemoglobin F level were in remission and in one not in remission refrectary anaemia preceded LAM. The results presented indicate that during the recovery of erythropoiesis, some erythroblasts synthetize haemoglobin F. Those erythroblasts are probably the progeny of erythroid stem cells which during differentiation and proliferation "skipp" some maturation stages.

In the first phase of treatment of acute lymphatic leukemia in children (ALL) with aggresive cytostatic protocols, the doctor is, in some patients, forced to modify the antitumor therapy over a certain period of time because of bone marrow depression. The authors attempted to pull patients with ALL through this critical phase of the disease - by administering "profilactically" Lithium Carbonate (Li2CO3) ( in order to stimulate granulopoiesis) or, if anaemia, leukopenia and thrombocytopenia had already occurred, by administering concentrates of erythrocytes, leukocytes and platelets - without discontinuing the administration of cytostatics. The results of these attempts are reported.

The cytostatics used in treatment of malignant diseases may, similar to other drugs sensibilize the organism and cause various allergic manifestations. The authors present 6 children with malignant diseases in whom various allergic reactions, from urticaria to a severe form of Stivens-Johnson syndrome, were observed to appear during the treatment with cytostatics. All symptoms of allergy withdrew after the administration of antihistaminics. Difficulties in differential diagnosis of an allergy and tocsic effects of cytostatics are also pointed out.