Studying the combining ability of pro-vitamin A enriched yellow maize inbred lines (Zea mays L.) provides valuable information on the breeding potential of these lines for developing high-yielding and biofortified maize hybrids with desirable agronomic traits. Twelve pro-vitamin A-enriched yellow maize inbred lines were crossed using a half diallel mating design with the objective of identifying the best combiners to use as testers and selecting the highest-performing hybrids for grain yield with desirable traits. Sixty-eight F1 hybrids along with checks were evaluated across three environments at mid-altitude agroecology of Ethiopia during the 2022 main cropping season. Combined analysis of variance revealed significant differences (p ≤ 0.01) due to hybrids, environments, and hybrid × environment interactions for grain yield and agronomic traits, indicating that genotypes performed differently across environments. Eight hybrids were selected based on superior mean yield performance (6.92–7.95 t ha−1), providing yield advantages of 15.6%–32.7% over the best check, warranting promotion to the next stage of hybrid evaluation. Analysis of variance for general combining ability (GCA) and specific combining ability showed significant differences (p ≤ 0.001) for all traits studied, indicating that these traits are controlled by both additive and non-additive gene effects. Inbred lines L2, L6, L8, L10, and L12 exhibited high, positive, significant GCA effects on yield and other traits. Inbred lines L10 and L12 showed the best combiners for most traits and exhibited high GCA effect for yield. Their crosses generated the top yield performance and negative contributions to foliar diseases. Thus, these inbred lines can be used as testers for future provitamin A maize breeding program in the mid-altitude agroecology of Ethiopia.
研究富含维生素A原的黄玉米自交系(Zea mays L.)的配合力,为培育具有理想农学性状的高产生物强化玉米杂交种提供了有价值的信息。采用半双列杂交设计,对12个富含维生素a原的黄玉米自交系进行杂交,以确定最佳的配合者作为试验材料,并选择具有理想性状、产量最高的杂交品种。在2022年主要种植季节,对68个F1杂交种进行了评估,并对其进行了检查。综合方差分析显示,杂交种、环境和杂交种与环境互作对籽粒产量和农艺性状的影响显著(p≤0.01),表明基因型在不同环境下表现不同。根据平均产量表现(6.92 ~ 7.95 t ha−1),选择了8个杂交品种,比最佳品种的产量优势为15.6% ~ 32.7%,可以进入下一阶段的杂交评价。一般配合力(GCA)和特异配合力的方差分析显示,各性状间差异显著(p≤0.001),说明这些性状同时受加性和非加性基因效应的控制。自交系L2、L6、L8、L10和L12在产量和其他性状上表现出高、正、显著的GCA效应。自交系L10和L12在大多数性状上表现为最佳组合,在产量上表现出较高的GCA效应。它们的杂交产生了最高的产量表现,对叶面病害的贡献为负。因此,这些自交系可以作为未来埃塞俄比亚中高海拔农业生态中维生素A原玉米育种计划的试验材料。
{"title":"Combining ability among provitamin A-enriched maize inbred lines and heterosis of their hybrids for grain yield and agronomic traits","authors":"Belay Garoma, Kassahun Bante, Girum Azmach, Abebe Menkir","doi":"10.1002/agg2.70303","DOIUrl":"https://doi.org/10.1002/agg2.70303","url":null,"abstract":"<p>Studying the combining ability of pro-vitamin A enriched yellow maize inbred lines (<i>Zea mays</i> L.) provides valuable information on the breeding potential of these lines for developing high-yielding and biofortified maize hybrids with desirable agronomic traits. Twelve pro-vitamin A-enriched yellow maize inbred lines were crossed using a half diallel mating design with the objective of identifying the best combiners to use as testers and selecting the highest-performing hybrids for grain yield with desirable traits. Sixty-eight F<sub>1</sub> hybrids along with checks were evaluated across three environments at mid-altitude agroecology of Ethiopia during the 2022 main cropping season. Combined analysis of variance revealed significant differences (<i>p</i> ≤ 0.01) due to hybrids, environments, and hybrid × environment interactions for grain yield and agronomic traits, indicating that genotypes performed differently across environments. Eight hybrids were selected based on superior mean yield performance (6.92–7.95 t ha<sup>−</sup><sup>1</sup>), providing yield advantages of 15.6%–32.7% over the best check, warranting promotion to the next stage of hybrid evaluation. Analysis of variance for general combining ability (GCA) and specific combining ability showed significant differences (<i>p</i> ≤ 0.001) for all traits studied, indicating that these traits are controlled by both additive and non-additive gene effects. Inbred lines L2, L6, L8, L10, and L12 exhibited high, positive, significant GCA effects on yield and other traits. Inbred lines L10 and L12 showed the best combiners for most traits and exhibited high GCA effect for yield. Their crosses generated the top yield performance and negative contributions to foliar diseases. Thus, these inbred lines can be used as testers for future provitamin A maize breeding program in the mid-altitude agroecology of Ethiopia.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147268977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sesame (Sesamum indicum L.) is an important oil crop in the world. A significant genotype by environmental interaction (GEI) presents challenges in the selection of superior genotypes. The study was initiated to identify stable and high-yielding sesame genotypes. Accordingly, 16 sesame genotypes were evaluated in a randomized complete block design at six distinct environments. All-important data were collected and analyzed using Statistical Analysis Software (SAS). Most of the analyzed traits showed highly significant differences for genotypes, location, and genotype by environment (GE) interaction, which indicated the presence of genetic variability among tested genotypes across environments. Stability analysis was estimated by the additive main effects and multiplicative interaction (AMMI) model and genotype and genotype by environment (GGE) biplots. AMMI analysis of variance revealed highly significant (p < 0.01) variations among environments, genotypes, GE interaction, and four interaction principal component analyses (IPCAs). The first four IPCAs (IPCA1, IPCA2, IPCA3, and IPCA4) showed high significance and contributed 56.3%, 23.9%, 11.3%, and 2.3% of the total GE interaction, respectively. Genotypes G15 and G4 had higher with close to concentric circles indicating the wide adaptability over the tested environments and similar agroecology. In addition, the genotypes showed microbial blight resistance in western parts of Ethiopia and hence were selected as potential candidates for possible release in the study area.
{"title":"Genotype by environment interaction and seed yield stability study of Ethiopian sesame (Sesamum indicum L.) genotypes at Western Oromia","authors":"Feyera Takele, Alemeyehu Dabesa, Chemeda Daba","doi":"10.1002/agg2.70323","DOIUrl":"https://doi.org/10.1002/agg2.70323","url":null,"abstract":"<p>Sesame (<i>Sesamum indicum</i> L.) is an important oil crop in the world. A significant genotype by environmental interaction (GEI) presents challenges in the selection of superior genotypes. The study was initiated to identify stable and high-yielding sesame genotypes. Accordingly, 16 sesame genotypes were evaluated in a randomized complete block design at six distinct environments. All-important data were collected and analyzed using Statistical Analysis Software (SAS). Most of the analyzed traits showed highly significant differences for genotypes, location, and genotype by environment (GE) interaction, which indicated the presence of genetic variability among tested genotypes across environments. Stability analysis was estimated by the additive main effects and multiplicative interaction (AMMI) model and genotype and genotype by environment (GGE) biplots. AMMI analysis of variance revealed highly significant (<i>p</i> < 0.01) variations among environments, genotypes, GE interaction, and four interaction principal component analyses (IPCAs). The first four IPCAs (IPCA1, IPCA2, IPCA3, and IPCA4) showed high significance and contributed 56.3%, 23.9%, 11.3%, and 2.3% of the total GE interaction, respectively. Genotypes G15 and G4 had higher with close to concentric circles indicating the wide adaptability over the tested environments and similar agroecology. In addition, the genotypes showed microbial blight resistance in western parts of Ethiopia and hence were selected as potential candidates for possible release in the study area.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70323","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146216994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morphometric analysis is crucial for watershed management, especially for estimating infiltration rate and erosion characteristics. However, there is limited research on the relationship between morphometric characteristics and soil hydrological properties in the Kiltie watershed. The study is aimed at exploring the relationship between morphometric characteristics and soil hydrological properties in the Kiltie watershed. The study utilized the Shuttle Radar Topography Mission digital elevation model (30 × 30 m spatial resolution) and ArcGIS 10.7.1 software to extract and analyze morphometric parameters. A numerical scheme was used to evaluate soil hydrological properties in relation to morphometric parameters. The morphometric analysis reveals that drainage pattern was dendritic and the maximum stream order of the watershed was five. Total number of stream of all orders was 560, with a total length of 358.277 km. Out of all order, 53.04% were covered by the first order, 23.03% by the second order, 10% by third order, 9.29% by fourth order, and 4.64% by fifth order. The watershed's higher drainage density (19.21 km/m2) and ruggedness number (3.55) indicate higher erosion sensitivity and lower surface permeability and infiltration, indicating a more resilient ecosystem. The values obtained through morphometric analysis and numerical scheme classification showed the watershed had low surface soil permeability, low infiltration rate, and high runoff, with a total weight score below six. This study and scheme could be useful for planners and decision-makers as a baseline in implementing successful soil and water conservation strategies. For future studies, we recommended that the extent of the vulnerability to erosion-prone areas should be identified through multi-criteria decision-making methodologies.
{"title":"Morphometric characteristics and their influence on soil hydrological properties in Kiltie watershed, Upper Blue Nile Basin, Ethiopia","authors":"Getu Abey Denekewu, Derege Tsegaye Meshesha, Enyew Adgo Tsegaye","doi":"10.1002/agg2.70312","DOIUrl":"https://doi.org/10.1002/agg2.70312","url":null,"abstract":"<p>Morphometric analysis is crucial for watershed management, especially for estimating infiltration rate and erosion characteristics. However, there is limited research on the relationship between morphometric characteristics and soil hydrological properties in the Kiltie watershed. The study is aimed at exploring the relationship between morphometric characteristics and soil hydrological properties in the Kiltie watershed. The study utilized the Shuttle Radar Topography Mission digital elevation model (30 × 30 m spatial resolution) and ArcGIS 10.7.1 software to extract and analyze morphometric parameters. A numerical scheme was used to evaluate soil hydrological properties in relation to morphometric parameters. The morphometric analysis reveals that drainage pattern was dendritic and the maximum stream order of the watershed was five. Total number of stream of all orders was 560, with a total length of 358.277 km. Out of all order, 53.04% were covered by the first order, 23.03% by the second order, 10% by third order, 9.29% by fourth order, and 4.64% by fifth order. The watershed's higher drainage density (19.21 km/m2) and ruggedness number (3.55) indicate higher erosion sensitivity and lower surface permeability and infiltration, indicating a more resilient ecosystem. The values obtained through morphometric analysis and numerical scheme classification showed the watershed had low surface soil permeability, low infiltration rate, and high runoff, with a total weight score below six. This study and scheme could be useful for planners and decision-makers as a baseline in implementing successful soil and water conservation strategies. For future studies, we recommended that the extent of the vulnerability to erosion-prone areas should be identified through multi-criteria decision-making methodologies.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146216907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A participatory field experiment was conducted during the 2024 main cropping season in collaboration with local farmers who jointly managed and evaluated the trials. The experiment aims to evaluate the effects of biochar-based fertilizer (BBF) on soil properties and bread wheat (Triticum aestivum) productivity grown on acidic Nitisols. Three fertilizer treatments (sole BBF, BBF plus 50% recommended nitrogen (N) rate (46 kg N ha−1), and 100% recommended N rate (92 kg N ha−1) were arranged in a randomized complete block across 18 farmers’ fields, each representing a replication site. Based on the initial soil pH results, the experimental fields were classified into three acidity levels (strong, moderate, and slight). Postharvest analysis results revealed that BBF combined with 50% recommended N fertilizer improved soil pH, organic carbon, and total nitrogen contents, compared to 100% recommended N fertilizer. Furthermore, the combined application of BBF with 50% recommended N fertilizer consistently yielded the highest wheat grain production across all acidity levels. On average, the combined treatment surpassed 100% recommended N fertilizer by 49%, 25.9%, and 17.5% in strong, moderate, and slightly acidic conditions, respectively. The effects of BBF application, either alone or in combination with 50% recommended N fertilizer, on soil properties, wheat yield, and yield components were more pronounced in strongly acidic soil, followed by moderate and slightly acidic conditions. Overall, integrating BBF with 50% recommended N fertilizer improved soil fertility and wheat productivity, suggesting a sustainable soil management option for acidic soils; however, further multi-season validation is recommended to confirm these findings.
在2024年主要种植季节,与共同管理和评估试验的当地农民合作,进行了一项参与性田间试验。本试验旨在评价生物炭基肥料(BBF)对酸性硝土上生长的面包小麦(Triticum aestivum)土壤性状和产量的影响。在18个农户的农田中随机设置3个施肥处理(单施BBF、BBF加50%推荐施氮量(46 kg N ha−1)和100%推荐施氮量(92 kg N ha−1),每个处理代表一个重复位点。根据初始土壤pH值,将试验田分为三个酸度等级(强、中、弱)。采后分析结果显示,与100%推荐施氮相比,BBF配施50%推荐施氮可改善土壤pH值、有机碳和全氮含量。此外,在所有酸度水平下,BBF配施50%推荐氮肥的小麦籽粒产量始终最高。在强酸性、中酸性和微酸性条件下,联合处理比100%推荐施氮量平均高出49%、25.9%和17.5%。在强酸性土壤中,单施或配施50%推荐氮肥对土壤性质、小麦产量和产量构成的影响最为显著,其次是中酸性和微酸性土壤。总体而言,将BBF与50%推荐氮肥相结合可提高土壤肥力和小麦生产力,为酸性土壤提供了可持续的土壤管理选择;然而,建议进一步的多季节验证来证实这些发现。
{"title":"Participatory evaluation of biochar-based fertilizer application: Effects on soil properties, yield, and yield components of bread wheat in acidic Nitisols","authors":"Ashenafi Nigussie, Kassu Tadess, Dejene Abera, Betelhem Mekonnen, Ribka Mekuria, Temesgen Desalegn, Reshid Abafita, Tolera Abera, Melkamu Hordofa","doi":"10.1002/agg2.70309","DOIUrl":"https://doi.org/10.1002/agg2.70309","url":null,"abstract":"<p>A participatory field experiment was conducted during the 2024 main cropping season in collaboration with local farmers who jointly managed and evaluated the trials. The experiment aims to evaluate the effects of biochar-based fertilizer (BBF) on soil properties and bread wheat (Triticum aestivum) productivity grown on acidic Nitisols. Three fertilizer treatments (sole BBF, BBF plus 50% recommended nitrogen (N) rate (46 kg N ha<sup>−1</sup>), and 100% recommended N rate (92 kg N ha<sup>−1</sup>) were arranged in a randomized complete block across 18 farmers’ fields, each representing a replication site. Based on the initial soil pH results, the experimental fields were classified into three acidity levels (strong, moderate, and slight). Postharvest analysis results revealed that BBF combined with 50% recommended N fertilizer improved soil pH, organic carbon, and total nitrogen contents, compared to 100% recommended N fertilizer. Furthermore, the combined application of BBF with 50% recommended N fertilizer consistently yielded the highest wheat grain production across all acidity levels. On average, the combined treatment surpassed 100% recommended N fertilizer by 49%, 25.9%, and 17.5% in strong, moderate, and slightly acidic conditions, respectively. The effects of BBF application, either alone or in combination with 50% recommended N fertilizer, on soil properties, wheat yield, and yield components were more pronounced in strongly acidic soil, followed by moderate and slightly acidic conditions. Overall, integrating BBF with 50% recommended N fertilizer improved soil fertility and wheat productivity, suggesting a sustainable soil management option for acidic soils; however, further multi-season validation is recommended to confirm these findings.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70309","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146216748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acidic soils in Ethiopia's Hadiya Zone limit crop production through aluminum toxicity and phosphorus (P) fixation, which constrain camelina potential. Though drought tolerant, camelina needs sufficient P. Triple superphosphate (TSP) often becomes ineffective because P is rapidly fixed; coffee husk biochar may reduce fixation and improve P availability. Field experiments examined combinations of amendments to test practical options for smallholder farmers in Hadiya, Ethiopia. This study evaluated camelina growth, yield, and P use efficiency under coffee husk biochar and TSP in Hadiya. A split-split-plot design with three replications tested two cultivars (Zeytee-1 and Syria), four TSP rates (0, 23, 46, and 69 kg ha−1), and four biochar rates (0, 10, 12, and 14 t ha−1). Biochar significantly enhanced growth: 14 t ha−1 increased leaf area by 48% and plant height by 39%, improving vigor. Combined biochar and TSP maximized yield components; Syria doubled siliques per plant with 69 kg ha−1 TSP plus biochar. Peak grain yields reached 2.64 t ha−1 for Syria (46 kg ha−1 TSP + 12 t ha−1 biochar) and 2.60 t ha−1 for Zeytee-1 (69 kg ha−1 TSP + 14 t ha−1 biochar). Biochar raised phosphorus recovery efficiency to 10.8% when combined with 23 kg ha−1 TSP. Cultivar responses differed: Zeytee-1 favored higher inputs; Syria performed best with balanced fertilization. Applying 12–14 t ha−1 coffee husk biochar with TSP (Zeytee-1: 46–69; Syria: 23–46 kg ha−1) enhances phosphorus efficiency. This practice improves marginal soil productivity and supports camelina as a viable oilseed for acidic soils.
埃塞俄比亚Hadiya地区的酸性土壤通过铝毒性和磷(P)固定限制了作物产量,这限制了亚麻荠的潜力。虽然耐干旱,但亚麻荠需要足够的磷,三重过磷酸钾(TSP)往往无效,因为磷很快被固定;咖啡壳生物炭可减少固着,提高磷利用率。在埃塞俄比亚的哈迪亚,实地试验考察了各种修订的组合,以测试小农的实际选择。本研究评价了咖啡壳生物炭和TSP处理下黑茶树的生长、产量和磷利用效率。采用3个重复的分割小区设计,试验了2个品种(zeytei -1和Syria)、4种TSP率(0、23、46和69 kg ha -1)和4种生物炭率(0、10、12和14 t ha -1)。生物炭显著促进生长:14 t ha - 1使叶面积增加48%,株高增加39%,提高了活力。生物炭与TSP组合产量组分最大;叙利亚以69 kg / ha - 1 TSP加生物炭的方式使每株植物的硅油增加了一倍。叙利亚(46公斤公顷−1 TSP + 12吨公顷−1生物炭)和Zeytee-1(69公斤公顷−1 TSP + 14吨公顷−1生物炭)的最高粮食产量分别达到2.64吨公顷−1和2.60吨公顷−1。当生物炭与23 kg ha−1 TSP配合使用时,磷回收率达到10.8%。不同品种的反应不同:Zeytee-1倾向于更高的投入;叙利亚在均衡施肥的情况下表现最好。施用含TSP (zeytee - 1:46 - 69; Syria: 23-46 kg ha -1)的12-14 kg ha -1咖啡壳生物炭可提高磷效率。这种做法提高了边际土壤生产力,并支持亚麻荠作为酸性土壤的可行油籽。
{"title":"Enhancing camelina [Camelina sativa (L.) Crantz] productivity in acidic Ethiopian soils: Synergistic effects of coffee husk biochar and phosphorus","authors":"Daniel Manore, Shimelis Gizachew, Tewodros Ayalew","doi":"10.1002/agg2.70316","DOIUrl":"https://doi.org/10.1002/agg2.70316","url":null,"abstract":"<p>Acidic soils in Ethiopia's Hadiya Zone limit crop production through aluminum toxicity and phosphorus (P) fixation, which constrain camelina potential. Though drought tolerant, camelina needs sufficient P. Triple superphosphate (TSP) often becomes ineffective because P is rapidly fixed; coffee husk biochar may reduce fixation and improve P availability. Field experiments examined combinations of amendments to test practical options for smallholder farmers in Hadiya, Ethiopia. This study evaluated camelina growth, yield, and P use efficiency under coffee husk biochar and TSP in Hadiya. A split-split-plot design with three replications tested two cultivars (Zeytee-1 and Syria), four TSP rates (0, 23, 46, and 69 kg ha<sup>−</sup><sup>1</sup>), and four biochar rates (0, 10, 12, and 14 t ha<sup>−</sup><sup>1</sup>). Biochar significantly enhanced growth: 14 t ha<sup>−</sup><sup>1</sup> increased leaf area by 48% and plant height by 39%, improving vigor. Combined biochar and TSP maximized yield components; Syria doubled siliques per plant with 69 kg ha<sup>−</sup><sup>1</sup> TSP plus biochar. Peak grain yields reached 2.64 t ha<sup>−</sup><sup>1</sup> for Syria (46 kg ha<sup>−</sup><sup>1</sup> TSP + 12 t ha<sup>−</sup><sup>1</sup> biochar) and 2.60 t ha<sup>−</sup><sup>1</sup> for Zeytee-1 (69 kg ha<sup>−</sup><sup>1</sup> TSP + 14 t ha<sup>−</sup><sup>1</sup> biochar). Biochar raised phosphorus recovery efficiency to 10.8% when combined with 23 kg ha<sup>−</sup><sup>1</sup> TSP. Cultivar responses differed: Zeytee-1 favored higher inputs; Syria performed best with balanced fertilization. Applying 12–14 t ha<sup>−</sup><sup>1</sup> coffee husk biochar with TSP (Zeytee-1: 46–69; Syria: 23–46 kg ha<sup>−</sup><sup>1</sup>) enhances phosphorus efficiency. This practice improves marginal soil productivity and supports camelina as a viable oilseed for acidic soils.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70316","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146216749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chandler M. Arel, Kristofor R. Brye, Diego Della Lunga, Trenton L. Roberts
Alternative fertilizer-phosphorus (P) sources created by recycling excess nutrients from waste sources, such as struvite (MgNH4PO4·6H2O), require further research to determine their niche in agriculture and potential environmental implications. The objective of this study was to evaluate rice (Oryza sativa) response and greenhouse gas emissions intensity from a real-wastewater-derived and synthetic electrochemically precipitated struvite (ECSTReal and ECSTSyn, respectively) and a chemically precipitated struvite compared to monoammonium phosphate (MAP) and an unamended control grown under flood- and furrow-irrigated conditions in a P-deficient, silt-loam soil in the greenhouse. A pure-line rice variety was grown under delayed-flood irrigation for ∼155 days in 2022, and a hybrid rice variety was grown for ∼170 days under simulated furrow-irrigation in 2023. In both 2022 and 2023, aboveground dry matter did not differ among P-fertilized rice treatments. Total plant P uptake in 2022 from MAP was 34.2 kg ha−1, which was similar to all struvite fertilizer-P sources. In 2023, aboveground P uptake from ECSTReal was numerically greatest at 19.8 kg ha−1 and similar to ECSTSyn and MAP at 18.6 and 17.9 kg ha−1, respectively. The ECSTReal and ECSTSyn sources were similar among fertilizer-P sources for all measured or calculated rice properties, except for methane emissions intensity in 2022 and aboveground tissue-N concentration and uptake, total aboveground N uptake, and total plant P uptake in 2023. Results emphasized that the use of struvite could provide a viable fertilizer-P source for both flood- and furrow-irrigated rice production systems.
通过回收废物来源(如鸟粪石(MgNH4PO4·6H2O))中的过量营养物质产生的替代肥料-磷(P)源,需要进一步研究以确定其在农业中的生态位和潜在的环境影响。本研究的目的是评价水稻(Oryza sativa)对实际废水和合成电化学沉淀鸟粪石(ECSTReal和ECSTSyn)以及化学沉淀鸟粪石(chem沉淀物)的响应和温室气体排放强度,并将其与磷酸铵(MAP)和未经改良的对照进行比较,这些对照在涝灌和沟灌条件下生长在缺磷的粉质壤土中。2022年,在延迟漫灌下种植了纯系水稻品种~ 155天,2023年在模拟沟灌下种植了杂交水稻品种~ 170天。在2022年和2023年,施磷水稻处理的地上部干物质没有差异。2022年MAP对植物磷的吸收总量为34.2 kg ha - 1,与所有鸟粪石磷肥源相似。2023年,ECSTReal的地上磷吸收量最大,为19.8 kg ha - 1,与ecstasy syn和MAP相似,分别为18.6和17.9 kg ha - 1。除了2022年的甲烷排放强度和2023年的地上组织氮浓度和吸收量、地上总氮吸收量和植株总磷吸收量不同外,ECSTReal和ecstasy syn源在所有测量或计算的水稻性状中都是相似的。结果强调鸟粪石的使用可以为水涝和沟灌水稻生产系统提供一种可行的肥磷来源。
{"title":"Rice response as affected by various fertilizer-P sources under contrasting irrigation methods","authors":"Chandler M. Arel, Kristofor R. Brye, Diego Della Lunga, Trenton L. Roberts","doi":"10.1002/agg2.70307","DOIUrl":"https://doi.org/10.1002/agg2.70307","url":null,"abstract":"<p>Alternative fertilizer-phosphorus (P) sources created by recycling excess nutrients from waste sources, such as struvite (MgNH<sub>4</sub>PO<sub>4</sub>·6H<sub>2</sub>O), require further research to determine their niche in agriculture and potential environmental implications. The objective of this study was to evaluate rice (<i>Oryza sativa</i>) response and greenhouse gas emissions intensity from a real-wastewater-derived and synthetic electrochemically precipitated struvite (ECST<sub>Real</sub> and ECST<sub>Syn</sub>, respectively) and a chemically precipitated struvite compared to monoammonium phosphate (MAP) and an unamended control grown under flood- and furrow-irrigated conditions in a P-deficient, silt-loam soil in the greenhouse. A pure-line rice variety was grown under delayed-flood irrigation for ∼155 days in 2022, and a hybrid rice variety was grown for ∼170 days under simulated furrow-irrigation in 2023. In both 2022 and 2023, aboveground dry matter did not differ among P-fertilized rice treatments. Total plant P uptake in 2022 from MAP was 34.2 kg ha<sup>−1</sup>, which was similar to all struvite fertilizer-P sources. In 2023, aboveground P uptake from ECST<sub>Real</sub> was numerically greatest at 19.8 kg ha<sup>−1</sup> and similar to ECST<sub>Syn</sub> and MAP at 18.6 and 17.9 kg ha<sup>−1</sup>, respectively. The ECST<sub>Real</sub> and ECST<sub>Syn</sub> sources were similar among fertilizer-P sources for all measured or calculated rice properties, except for methane emissions intensity in 2022 and aboveground tissue-N concentration and uptake, total aboveground N uptake, and total plant P uptake in 2023. Results emphasized that the use of struvite could provide a viable fertilizer-P source for both flood- and furrow-irrigated rice production systems.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70307","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146193708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Niloy Majumder, Md. Akhter Hossain Chowdhury, Rakibul Hasan Md. Rabbi, Sanjida Yeasmin Etu, Biplob Kumar Saha
Aloe vera (Aloe vera L.) has been utilized for various therapeutic and cosmetic purposes; however, its agronomic management in Bangladesh remains underexplored. Therefore, a field trial was performed in Mymensingh, Bangladesh, to evaluate the combined effects of organic and inorganic fertilizers (IFs) on growth, nutrient composition, medicinal components, and post-harvest soil fertility of A. vera. Nine treatments consisting of organic matter (OM) (cow dung [CD] and poultry manure [PM]) and IFs, namely, IF0OM0, IF100OM0, IF85OM15, IF70OM30, IF55OM45, IF40OM60, IF25OM75, IF10OM90, and IF0OM100, were laid out in a Randomized Complete Block Design with three replicates. The results of the study confirmed that integrated nutrient management substantially enhanced plant performance with the IF25OM75 treatment in both CD and PM combinations, including the tallest plants, the highest leaf number, the largest leaf area, and the most suckers. Interestingly, CD containing integrated fertilizer produced about 14% more leaf biomass than PM containing integrated fertilizer, while PM-containing treatments increased nutrient (N, P, K, Fe, Zn, and Mn) acquisition more effectively than CD-based fertilizer. Biochemical assessments revealed that protein content peaked under IF100OM0. In contrast, chlorophyll, aloin, flavonoids, and phenolics were significantly higher in OM-dominant treatments than IF, especially IF0OM100 in both CD and PM containing fertilizer, which produced the highest aloin (494 µg g−1) and phenolics (29.35 mg gallic acid equivalents g−1 fresh weight content. The post-harvest soil analysis revealed that higher OM, particularly PM, significantly enhanced soil fertility by increasing organic matter, N, P, S, Zn, and exchangeable K, Ca, and Mg compared with CD containing integrated fertilizer. Overall, the study suggested that integrating OM with moderate IF, especially IF25OM75 for both CD and PM, can improve growth, nutritional value, medicinal properties, and soil fertility of A. vera.
芦荟(Aloe vera L.)已被用于各种治疗和美容目的;然而,其在孟加拉国的农艺管理仍未得到充分探索。因此,在孟加拉国Mymensingh进行了一项田间试验,以评估有机和无机肥料(IFs)对缬草生长、营养成分、药用成分和收获后土壤肥力的联合影响。试验采用随机完全区组设计,采用牛粪(CD)和禽粪(PM) 9个处理,分别为IF0OM0、IF100OM0、IF85OM15、IF70OM30、IF55OM45、IF40OM60、IF25OM75、IF10OM90和IF0OM100,共3个重复。研究结果证实,综合营养管理显著提高了IF25OM75处理在CD和PM组合下的植株性能,包括最高植株、最高叶数、最大叶面积和最多吸盘。有趣的是,含CD的综合肥料比含PM的综合肥料产生的叶片生物量多14%,而含PM的处理比含CD的肥料更有效地增加了养分(N, P, K, Fe, Zn和Mn)的获取。生化分析结果显示,IF100OM0处理下蛋白质含量最高。相比之下,有机肥处理的叶绿素、芦荟素、黄酮类化合物和酚类物质含量显著高于有机肥处理,特别是有机肥和有机肥处理IF0OM100的芦荟素(494µg g−1)和酚类物质(29.35 mg没食子酸相当于g−1鲜重)含量最高。收获后土壤分析表明,与含CD的综合肥料相比,较高的有机质,特别是PM,通过增加有机质、N、P、S、Zn和交换性K、Ca、Mg,显著提高了土壤肥力。综上所述,OM与适量IF结合,特别是IF25OM75对CD和PM均有促进黄芪生长、营养价值、药用特性和土壤肥力的作用。
{"title":"Synergistic effect of organic and inorganic fertilizers on Aloe vera: Enhancing leaf biomass, nutrient uptake, medicinal properties, and soil fertility","authors":"Niloy Majumder, Md. Akhter Hossain Chowdhury, Rakibul Hasan Md. Rabbi, Sanjida Yeasmin Etu, Biplob Kumar Saha","doi":"10.1002/agg2.70306","DOIUrl":"https://doi.org/10.1002/agg2.70306","url":null,"abstract":"<p>Aloe vera (<i>Aloe vera</i> L.) has been utilized for various therapeutic and cosmetic purposes; however, its agronomic management in Bangladesh remains underexplored. Therefore, a field trial was performed in Mymensingh, Bangladesh, to evaluate the combined effects of organic and inorganic fertilizers (IFs) on growth, nutrient composition, medicinal components, and post-harvest soil fertility of <i>A. vera</i>. Nine treatments consisting of organic matter (OM) (cow dung [CD] and poultry manure [PM]) and IFs, namely, IF<sub>0</sub>OM<sub>0</sub>, IF<sub>100</sub>OM<sub>0</sub>, IF<sub>85</sub>OM<sub>15</sub>, IF<sub>70</sub>OM<sub>30</sub>, IF<sub>55</sub>OM<sub>45</sub>, IF<sub>40</sub>OM<sub>60</sub>, IF<sub>25</sub>OM<sub>75</sub>, IF<sub>10</sub>OM<sub>90</sub>, and IF<sub>0</sub>OM<sub>100</sub>, were laid out in a Randomized Complete Block Design with three replicates. The results of the study confirmed that integrated nutrient management substantially enhanced plant performance with the IF<sub>25</sub>OM<sub>75</sub> treatment in both CD and PM combinations, including the tallest plants, the highest leaf number, the largest leaf area, and the most suckers. Interestingly, CD containing integrated fertilizer produced about 14% more leaf biomass than PM containing integrated fertilizer, while PM-containing treatments increased nutrient (N, P, K, Fe, Zn, and Mn) acquisition more effectively than CD-based fertilizer. Biochemical assessments revealed that protein content peaked under IF<sub>100</sub>OM<sub>0</sub>. In contrast, chlorophyll, aloin, flavonoids, and phenolics were significantly higher in OM-dominant treatments than IF, especially IF<sub>0</sub>OM<sub>100</sub> in both CD and PM containing fertilizer, which produced the highest aloin (494 µg g<sup>−</sup><sup>1</sup>) and phenolics (29.35 mg gallic acid equivalents g<sup>−</sup><sup>1</sup> fresh weight content. The post-harvest soil analysis revealed that higher OM, particularly PM, significantly enhanced soil fertility by increasing organic matter, N, P, S, Zn, and exchangeable K, Ca, and Mg compared with CD containing integrated fertilizer. Overall, the study suggested that integrating OM with moderate IF, especially IF<sub>25</sub>OM<sub>75</sub> for both CD and PM<sub>,</sub> can improve growth, nutritional value, medicinal properties, and soil fertility of <i>A. vera</i>.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70306","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146193709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alison J. Duff, Kevin Panke-Buisse, Jonathan S. Bleier, Geoffrey E. Brink
Productive forage with sufficient nutritive value is essential to profitable grazing operations. Application of nitrogen (N) fertilizer directly impacts pasture growth, but N rates must be carefully calibrated to minimize production costs and losses to the environment. We evaluated forage accumulation response, nitrogen use efficiency (NUE), and % 15N recovery of meadow fescue [Schedonorus pratensis (Huds.) P. Beauv.], orchardgrass (Dactylis glomerata L.), and endophyte-free tall fescue [Schedonorus phoenix (Scop.) Holub] in response to N rate in a controlled greenhouse environment using two soil types. Nitrogen was split-applied at cumulative annual rates of 0, 67, 135, 202, 269, and 336 kg N/ha. The first N application was 15N-enriched 5% atom ammonium nitrate (NH4NO3) solution, while the three subsequent N applications were made following harvest events with reagent-grade NH4NO3. Nitrogen recovery from the first application was estimated from the atom % 15N in the dry matter (DM) sample from each harvest event. For all species, DM forage accumulation increased with N rate up to 202 kg N/ha, and orchardgrass had the greatest DM accumulation at all N rates. NUE in all species declined with increased N. % 15N recovery of the first fertilizer application was significantly different across species and N rates, ranging from 35% to 70%. In pasture systems with these species in warm summer continental climates, N fertilizer timing and application strategy should be calibrated with weather, pasture conditions, and livestock demand.
具有足够营养价值的生产性牧草对盈利的放牧作业至关重要。氮肥的施用直接影响牧草的生长,但氮肥用量必须仔细校准,以尽量减少生产成本和对环境的损失。以草甸羊茅(Schedonorus pratensis, Huds)为研究对象,对牧草积累响应、氮素利用效率(NUE)和% 15N恢复进行了评价。测定。],果园草(Dactylis glomerata L.)和无内生菌的高羊茅[scheonorus phoenix (Scop.)]。Holub]在两种土壤类型的受控温室环境下对氮含量的响应。施氮量分别为0、67、135、202、269和336 kg N/ha。第一次施氮是15n富集的5%硝酸铵(NH4NO3)溶液,而随后的三次施氮是在收获事件后用试剂级NH4NO3进行的。从每次收获事件的干物质(DM)样品中的原子% 15N估计第一次施用的氮回收率。各树种的DM积累量均随施氮量的增加而增加,最高可达202 kg N/ hm2,在各施氮量下,果园草的DM积累量最大。各树种氮素利用效率随施氮量的增加而下降,不同树种和施氮量的氮素回收率差异显著,在35% ~ 70%之间。在温暖的夏季大陆性气候条件下,有这些物种的牧场系统,应根据天气、牧场条件和牲畜需求调整氮肥的施用时间和施用策略。
{"title":"Evaluation of forage grass response to nitrogen rate in a controlled greenhouse study","authors":"Alison J. Duff, Kevin Panke-Buisse, Jonathan S. Bleier, Geoffrey E. Brink","doi":"10.1002/agg2.70308","DOIUrl":"https://doi.org/10.1002/agg2.70308","url":null,"abstract":"<p>Productive forage with sufficient nutritive value is essential to profitable grazing operations. Application of nitrogen (N) fertilizer directly impacts pasture growth, but N rates must be carefully calibrated to minimize production costs and losses to the environment. We evaluated forage accumulation response, nitrogen use efficiency (NUE), and % <sup>15</sup>N recovery of meadow fescue [<i>Schedonorus pratensis</i> (Huds.) P. Beauv.], orchardgrass (<i>Dactylis glomerata</i> L.), and endophyte-free tall fescue [<i>Schedonorus phoenix</i> (Scop.) Holub] in response to N rate in a controlled greenhouse environment using two soil types. Nitrogen was split-applied at cumulative annual rates of 0, 67, 135, 202, 269, and 336 kg N/ha. The first N application was <sup>15</sup>N-enriched 5% atom ammonium nitrate (NH<sub>4</sub>NO<sub>3</sub>) solution, while the three subsequent N applications were made following harvest events with reagent-grade NH<sub>4</sub>NO<sub>3</sub>. Nitrogen recovery from the first application was estimated from the atom % <sup>15</sup>N in the dry matter (DM) sample from each harvest event. For all species, DM forage accumulation increased with N rate up to 202 kg N/ha, and orchardgrass had the greatest DM accumulation at all N rates. NUE in all species declined with increased N. % <sup>15</sup>N recovery of the first fertilizer application was significantly different across species and N rates, ranging from 35% to 70%. In pasture systems with these species in warm summer continental climates, N fertilizer timing and application strategy should be calibrated with weather, pasture conditions, and livestock demand.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70308","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146193716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sebastián Bonansea, Oswaldo Ernst, Pablo González Barrios, Sebastián R. Mazzilli
Improving nitrogen (N) use efficiency in Brassica carinata is essential to sustain yield and lower biofuel carbon intensity under variable field conditions. We hypothesized that integrating physiological indicators, such as the nitrogen nutrition index (NNI) and the critical nitrogen uptake curve (CriNUC), into N management frameworks would better discriminate crop N status and improve the diagnostic evaluation of fertilizer performance in B. carinata under temperate field conditions. A 2-year, multi-site field experiment in Uruguay evaluated conventional split urea (CONTROL), slow-release, and nitrification-inhibited fertilizers (ENTEC 150 and Sulfammo-NPRO) at 0, 90, and 150 kg N ha−1. Crop responses were assessed through yield components, nitrogen uptake, nitrogen use efficiency (NUE) metrics (agronomic efficiency of nitrogen [AEN], recovery efficiency of nitrogen [REN], and IEN), and physiological indicators (NNI and CriNUC). ENTEC 150 increased grain yield by 70% relative to the CONTROL and consistently maintained N sufficiency from elongation to flowering. Across treatments, NNI and CriNUC provided complementary, in-season insights that were not captured by static NUE indices, particularly under environmental stress. Neither REN nor AEN correlated with NNI at flowering, highlighting potential physiological or structural constraints (e.g., lodging) that limit predictability under field conditions. Treatments with NNI > 1 showed increased protein and reduced oil concentration, confirming trade-offs between productivity and grain composition. While results are exploratory due to the use of a generic dilution curve, our findings suggest that combining enhanced-efficiency fertilizers with crop-based diagnostics supports more precise and adaptive nitrogen management in B. carinata. Further calibration is needed to refine these tools and validate their utility for decision-making across diverse environments.
在多变的田间条件下,提高油菜氮素利用效率对维持产量和降低生物燃料碳强度至关重要。我们假设将氮营养指数(NNI)和临界氮吸收曲线(CriNUC)等生理指标整合到氮素管理框架中,可以更好地区分作物氮素状况,提高对温带大田条件下牛蒡肥效的诊断评价。乌拉圭进行了一项为期2年的多地点田间试验,评估了0、90和150 kg N ha - 1下常规分裂尿素(CONTROL)、缓释和硝化抑制肥料(ENTEC 150和磺胺- npro)的效果。通过产量组成、氮素吸收、氮素利用效率(NUE)指标(氮素农艺效率[AEN]、氮素恢复效率[REN]和氮素利用率])和生理指标(NNI和CriNUC)来评估作物的响应。与对照相比,ENTEC 150提高了70%的籽粒产量,并从伸长到开花始终保持氮充足。在整个处理过程中,NNI和CriNUC提供了静态NUE指数无法捕获的互补的季节性见解,特别是在环境压力下。在花期,REN和AEN都与NNI无关,这突出了潜在的生理或结构限制(如倒伏),限制了田间条件下的可预测性。NNI >; 1处理显示蛋白质含量增加,油浓度降低,证实了生产力和籽粒组成之间的权衡。虽然由于使用了通用稀释曲线,结果是探索性的,但我们的研究结果表明,将高效肥料与基于作物的诊断相结合,可以支持对海苔进行更精确和适应性的氮管理。需要进一步的校准来完善这些工具,并验证它们在不同环境下决策的实用性。
{"title":"Field assessment of nitrogen sources in Brassica carinata using physiological indicators of nitrogen status under temperate no-till conditions","authors":"Sebastián Bonansea, Oswaldo Ernst, Pablo González Barrios, Sebastián R. Mazzilli","doi":"10.1002/agg2.70301","DOIUrl":"https://doi.org/10.1002/agg2.70301","url":null,"abstract":"<p>Improving nitrogen (N) use efficiency in <i>Brassica carinata</i> is essential to sustain yield and lower biofuel carbon intensity under variable field conditions. We hypothesized that integrating physiological indicators, such as the nitrogen nutrition index (NNI) and the critical nitrogen uptake curve (CriNUC), into N management frameworks would better discriminate crop N status and improve the diagnostic evaluation of fertilizer performance in <i>B. carinata</i> under temperate field conditions. A 2-year, multi-site field experiment in Uruguay evaluated conventional split urea (CONTROL), slow-release, and nitrification-inhibited fertilizers (ENTEC 150 and Sulfammo-NPRO) at 0, 90, and 150 kg N ha<sup>−1</sup>. Crop responses were assessed through yield components, nitrogen uptake, nitrogen use efficiency (NUE) metrics (agronomic efficiency of nitrogen [AE<sub>N</sub>], recovery efficiency of nitrogen [RE<sub>N</sub>], and IE<sub>N</sub>), and physiological indicators (NNI and CriNUC). ENTEC 150 increased grain yield by 70% relative to the CONTROL and consistently maintained N sufficiency from elongation to flowering. Across treatments, NNI and CriNUC provided complementary, in-season insights that were not captured by static NUE indices, particularly under environmental stress. Neither RE<sub>N</sub> nor AE<sub>N</sub> correlated with NNI at flowering, highlighting potential physiological or structural constraints (e.g., lodging) that limit predictability under field conditions. Treatments with NNI > 1 showed increased protein and reduced oil concentration, confirming trade-offs between productivity and grain composition. While results are exploratory due to the use of a generic dilution curve, our findings suggest that combining enhanced-efficiency fertilizers with crop-based diagnostics supports more precise and adaptive nitrogen management in <i>B. carinata</i>. Further calibration is needed to refine these tools and validate their utility for decision-making across diverse environments.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70301","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Garlic (Allium sativum), a vegetable crop used for food, medicine, and condiments, is affected by white rot (Sclerotium cepivorum). Garlic yield reduction is influenced by several factors, with biotic stresses, particularly fungal pathogens, being among the most significant. The main aim of this work was to examine the effect of bacterial antagonists in preventing or controlling the attack of white rot. Bacterial antagonists and the phytopathogens were isolated and characterized using the serial dilution technique and standard microbiological procedures. The biocontrol properties of bacterial isolates, including fungal inhibition, production of hydrolytic enzymes, and bioactive compounds, were determined. Similarly, the in vivo inhibition potential of 10 selected antagonists was evaluated under greenhouse conditions. Eleven isolates, accounting for 47.8%, inhibited the radial growth of white rot at disease incidences of 1%–4% and 33.3%, respectively. Among the bacterial isolates, 10 (90.9%) showed cellulase activity, while 6 (54.6%) and 5 (45.5%) were positive for chitinase and protease production, respectively. Furthermore, 11 (100%) and 5 (45.5%) of the isolates were positive for ammonia and hydrogen cyanide production, respectively. Garlic plants treated with bacteria produced 135.0%–142.2%, and 291.7%–370.8% greater height and weight, respectively. The isolates exhibited 99% sequence homology with Pantoea species, including Pantoea agglomerans, as well as Enterobacter species, Enterococcus species, and E. gallinarum. The budget was one limitation to further evaluating the effect of bacterial antagonists against white rot affecting garlic under field conditions. This finding indicated that the application of potential microbial inoculants can control plant disease. Consequently, isolates WUGRB-14 and -92 (where WUGRB is Wollo University Grasspea Rhizobacteria) can be recommended for field application as an alternative to chemical pesticides.
{"title":"Antagonistic effect of rhizospheric bacteria against white rot (Sclerotium cepivorum) of garlic (Allium sativum L.) under in vitro and in vivo conditions","authors":"Mussa Adal Mohammed, Asmare Dejen Demeke","doi":"10.1002/agg2.70300","DOIUrl":"https://doi.org/10.1002/agg2.70300","url":null,"abstract":"<p>Garlic (<i>Allium sativum)</i>, a vegetable crop used for food, medicine, and condiments, is affected by white rot (<i>Sclerotium cepivorum</i>). Garlic yield reduction is influenced by several factors, with biotic stresses, particularly fungal pathogens, being among the most significant. The main aim of this work was to examine the effect of bacterial antagonists in preventing or controlling the attack of white rot. Bacterial antagonists and the phytopathogens were isolated and characterized using the serial dilution technique and standard microbiological procedures. The biocontrol properties of bacterial isolates, including fungal inhibition, production of hydrolytic enzymes, and bioactive compounds, were determined. Similarly, the in vivo inhibition potential of 10 selected antagonists was evaluated under greenhouse conditions. Eleven isolates, accounting for 47.8%, inhibited the radial growth of white rot at disease incidences of 1%–4% and 33.3%, respectively. Among the bacterial isolates, 10 (90.9%) showed cellulase activity, while 6 (54.6%) and 5 (45.5%) were positive for chitinase and protease production, respectively. Furthermore, 11 (100%) and 5 (45.5%) of the isolates were positive for ammonia and hydrogen cyanide production, respectively. Garlic plants treated with bacteria produced 135.0%–142.2%, and 291.7%–370.8% greater height and weight, respectively. The isolates exhibited 99% sequence homology with <i>Pantoea</i> species, including <i>Pantoea agglomerans</i>, as well as <i>Enterobacter</i> species, <i>Enterococcus</i> species, and <i>E. gallinarum</i>. The budget was one limitation to further evaluating the effect of bacterial antagonists against white rot affecting garlic under field conditions. This finding indicated that the application of potential microbial inoculants can control plant disease. Consequently, isolates WUGRB-14 and -92 (where WUGRB is Wollo University Grasspea Rhizobacteria) can be recommended for field application as an alternative to chemical pesticides.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"9 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70300","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146176664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}