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Can conservation tillage practices improve soil characteristics in cornfields during a short-term period? 保护性耕作能在短期内改善玉米地的土壤特性吗?
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-25 DOI: 10.1002/agg2.70262
Abdolhossein Parandi, Gholamreza Mohammadi, Ali Beheshti Alagha, Mahmud Khoramivafa

The use of conventional tillage practices in crop production systems has led to a significant soil loss in many regions of Iran. Conservation tillage practices can help restore the degraded soils. A 2-year experiment was conducted in an irrigated corn (Zea mays L.) cropping system during the 2014–2016 growing seasons on a silty clay soil with a Mediterranean climatic condition. The experiment was carried out as a split plot based on a randomized complete block design with three replications. The main factor was the tillage system in three levels (conventional tillage, reduced tillage, and no tillage), and the sub-factor was the corn hybrid including KSC704, AS71, BC678, and Simon. Results showed that the surface layer of the soil (0- to 20-cm depth) was more affected by tillage systems, so that conservation tillage systems (reduced and no tillage) improved physical (including soil moisture contents at field capacity and permanent wilting points, the amount of water-dispersible clay, and mean weight diameter of the soil aggregates), chemical (including the amount of soil organic carbon, cation exchange capacity, total nitrogen, phosphorus, potassium, and iron), and biological (including microbial biomass carbon, basal and substrate-induced respiration, metabolic quotient, and the activity of soil extracellular enzymes) properties of the soil. For example, soil organic carbon under no and reduced tillage systems was higher by 28% and 22%, respectively, compared to conventional tillage. In general, the results revealed that tillage practices even in a relatively short-term period can notably affect soil characteristics.

在作物生产系统中使用传统耕作方法导致伊朗许多地区严重的土壤流失。保护性耕作有助于恢复退化的土壤。本试验于2014-2016年在地中海气候条件下的粉质粘土上进行灌溉玉米(Zea mays L.)种植系统2年试验。试验采用随机完全区组设计的分割图,设3个重复。主要因子为常规耕作、少耕和免耕3个层次的耕作制度,次因子为KSC704、AS71、BC678和Simon等玉米杂交种。结果表明:土壤表层(0 ~ 20 cm)受耕作制度的影响较大,保护性耕作制度(减耕和免耕)改善了土壤物理(包括田容量和永久萎蔫点土壤水分含量、水分散粘土量和土壤团聚体平均重径)、化学(包括土壤有机碳量、阳离子交换量、全氮、全磷、全钾和全铁)、以及土壤的生物特性(包括微生物生物量碳、基础和底物诱导的呼吸、代谢商和土壤细胞外酶的活性)。例如,免耕和减耕制度下的土壤有机碳分别比常规耕作高28%和22%。总体而言,研究结果表明,即使在较短的时间内耕作也会显著影响土壤特征。
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引用次数: 0
Grain yield stability by different statistical models in small seeded common bean (Phaseolus vulgaris L.) genotypes at diverse acroecologies of Ethiopia 埃塞俄比亚不同植物生态条件下小粒豆(Phaseolus vulgaris L.)基因型籽粒产量稳定性的不同统计模型
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-21 DOI: 10.1002/agg2.70253
Flagot Estifanos, Emana Getu, Dereje Beyene, Mesfin Hailemariam Habtegebriel, Berhanu Amsalu Fenta

Common bean (Phaseolus vulgaris L.) is an essential legume crop in Ethiopia with the potential to contribute to the agricultural system and food security. Since limited productivity is one of the main problems in the study region, stability analysis is essential to enhance productivity by identifying superior genotypes. Thus, this study was conducted to identify common bean genotypes that perform best and are stable at diverse agroecologies. The experiment was carried out on twenty-five small-seeded genotypes grown at four agroecologies for 2021–2022, using a triple lattice design. Evaluation was conducted on nine quantitative traits related to yield. To analyze the performance and stability of the genotypes, analysis of variance (ANOVA), AMMI (additive main effects and multiplicative interaction), AMMI stability value (ASV) rank, WAASB (weighted average of absolute scores biplot), genotype selection index (GSI), and GGE biplot analysis were used. The ANOVA revealed highly significant (p < 0.001) effects of genotypes, environment, and genotype-by-environment interaction for all traits, except the nonsignificant environmental effect for plant height and hundred seed weight. The result of AMMI indicated that Alemtena and Negele Arsi were stable environments and identified G22, G24, and G21 as stable genotypes. However, the GGE identified the mega-environments and best yielding common bean for each environment. The other statistical model, WAASB, identified Mieso as the most representative and discriminating environment, and GSI considered G11, G21, and G24 as desirable genotypes. Both AMMI and ASV identified G18, G21, and G24 as stable genotypes across the tested areas and are recommended for mega-environment production, and Alemtena as an ideal location for the selection of common bean genotypes, since it shows high representativeness and discrimination ability.

普通豆(Phaseolus vulgaris L.)是埃塞俄比亚重要的豆科作物,具有促进农业系统和粮食安全的潜力。由于有限的生产力是研究区域的主要问题之一,稳定性分析是通过鉴定优良基因型来提高生产力的必要条件。因此,本研究旨在鉴定在不同农业生态环境中表现最好且稳定的普通豆类基因型。该试验采用三重晶格设计,对2021-2022年在四个农业生态环境中生长的25个小种子基因型进行了试验。对与产量有关的9个数量性状进行了评价。采用方差分析(ANOVA)、AMMI(可加性主效应和乘法互作)、AMMI稳定性值(ASV)秩、WAASB(绝对得分加权平均双图)、基因型选择指数(GSI)和GGE双图分析各基因型的表现和稳定性。方差分析显示,除株高和百粒重的环境影响不显著外,基因型、环境和基因型-环境相互作用对所有性状的影响均极显著(p < 0.001)。AMMI结果表明Alemtena和Negele Arsi是稳定的环境,并鉴定出G22、G24和G21为稳定的基因型。然而,GGE确定了大型环境和每个环境下普通豆的最佳产量。另一个统计模型WAASB认为Mieso是最具代表性和歧视性的环境,GSI认为G11、G21和G24是理想的基因型。AMMI和ASV均将G18、G21和G24鉴定为稳定的基因型,推荐用于大环境生产,而Alemtena是选择普通豆类基因型的理想地点,具有较高的代表性和鉴别能力。
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引用次数: 0
Evaluation of sorghum [Sorghum bicolor (L.) Monech] genotypes for drought tolerance based on morpho-physiological traits 高粱的评价[高粱双色(L.)]基于形态生理性状的抗旱基因型
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-20 DOI: 10.1002/agg2.70254
Wedajo Gebre, Firew Mekbib, Alemu Tirfessa, Agidew Bekele

Sorghum [Sorghum bicolor (L.) Moench] is an important crop in Ethiopia, especially in lowland areas where drought stress is a major issue. Despite its tolerance, sorghum is susceptible to water-deficit stress during certain growth stages. To identify drought-tolerant genotypes and traits/mechanisms contributing to drought tolerance, a study evaluated 225 sorghum genotypes and assessed the variability, heritability, and genetic advance of these traits using a simple lattice design under stress and non-stress conditions. The analysis revealed significant differences in all traits under both conditions. Drought had a notable impact on various aspects of plant growth, including flowering, maturity, grain yield, and physiological traits such as chlorophyll content and canopy temperature. The study also found moderate to high genetic variation, genetic advance, and heritability for grain yield per panicle, panicle weight, plant height, panicle length, straw yield, and aboveground biomass under both environments. Additionally, grain yield had a positive correlation with 1000-kernel weight, aboveground biomass, harvest index, and grain yield per panicle under both conditions. These traits therefore deserve more attention in future breeding programs aimed at developing drought-tolerant sorghum varieties. The study underlined that morpho-physiological diversity in the studied material is shaped by genotypes, and it could be utilized for variety development and germplasm conservation programs aimed at improving drought tolerance in sorghum.

高粱[双色高粱]Moench是埃塞俄比亚的一种重要作物,特别是在干旱是主要问题的低地地区。尽管高粱具有耐受性,但在某些生长阶段易受缺水胁迫。为了确定抗旱基因型和抗旱性状/机制,本研究对225个高粱基因型进行了分析,并利用简单格设计对这些性状在胁迫和非胁迫条件下的变异性、遗传力和遗传进展进行了评估。分析显示,在两种条件下,所有性状都有显著差异。干旱对植物开花、成熟、产量以及叶绿素含量、冠层温度等生理性状的影响显著。在两种环境下,籽粒单穗产量、穗重、株高、穗长、秸秆产量和地上生物量的遗传变异、遗传进步和遗传力均为中高。籽粒产量与千粒重、地上生物量、收获指数和每穗产量均呈显著正相关。因此,这些性状应在今后培育耐旱高粱品种的育种计划中得到更多的关注。研究结果表明,所研究材料的形态生理多样性是由基因型决定的,可用于提高高粱抗旱性的品种开发和种质资源保护。
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引用次数: 0
Organic matter content influence on the hydraulic properties of sandy material 有机质含量对砂质材料水力性能的影响
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-16 DOI: 10.1002/agg2.70250
Adrien Cabrel Djomo Bouyem, Abdelkabir Maqsoud, Tikou Belem

The performance of the cover system, used in the reclamation of a mine site, is primarily assessed through hydraulic properties, including volumetric water content, suction, and saturated hydraulic conductivity (ksat). However, these properties may be influenced by factors such as soil mineralization (which refers to the process by which organic materials are converted into inorganic substances through natural processes), temperature (by the viscosity of water and the activity of microorganisms in the soil), and organic matter content (OMC), which complicate the accurate assessment of cover system performance. To better understand the impact of OMC on hydraulic properties, this study was initiated with two objectives: (i) evaluating the effect of OMC on the water retention curve (WRC) and ksat of a sandy material amended with peat and (ii) proposing equations to predict the WRC of sand amended with organic matter using the Fredlund and Xing model. This was accomplished through laboratory tests that determined the WRC and ksat of sand and sand amended with varying concentrations of peat (0%, 1%, 3%, 5%, 7.5%, 10%, 12.5%, and 15%). The investigation results indicate the air entry value (the suction at which the material begins to desaturate) evaluated using the sand mixture WRC did not show any notable variation. The ksat of the sand mixtures decreases with increasing peat concentration. In terms of prediction, the results obtained for the six mixtures tested in the laboratory showed an excellent agreement between predicted and experimental values, demonstrating the high accuracy with which the WRC s were predicted.

用于矿区复垦的覆盖系统的性能主要通过水力性能进行评估,包括体积含水量、吸力和饱和水力导电性(ksat)。然而,这些特性可能受到土壤矿化(指有机物质通过自然过程转化为无机物的过程)、温度(由水的粘度和土壤中微生物的活性)和有机质含量(OMC)等因素的影响,这些因素使覆盖系统性能的准确评估复杂化。为了更好地理解OMC对水力特性的影响,本研究有两个目标:(i)评估OMC对泥炭改性砂质材料的保水曲线(WRC)和ksat的影响;(ii)使用Fredlund和Xing模型提出公式来预测有机质改性砂的WRC。通过实验室测试,确定了不同泥炭浓度(0%、1%、3%、5%、7.5%、10%、12.5%和15%)下的砂和砂的WRC和ksat。调查结果表明,使用混合砂WRC评估的空气入口值(材料开始去饱和的吸力)没有显示出任何显着变化。混合砂的ksat随泥炭浓度的增加而降低。在预测方面,实验室测试的6种混合物的预测结果与实验值吻合良好,表明预测WRC具有较高的准确性。
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引用次数: 0
Effects of drought stress and zeolite application rates on yield, oil content, and antioxidant enzymes of sesame cultivars in arid regions of Iran 干旱胁迫和沸石施用量对伊朗干旱区芝麻产量、含油量和抗氧化酶的影响
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-14 DOI: 10.1002/agg2.70256
Mahnaz Narouei, Alireza Sirousmehr, Mehdi Dahmardeh, Esmaeel Seyedabadi

Drought stress is a significant environmental factor affecting sesame (Sesamum indicum L.) yield and quality. This study was conducted as a split-plot factorial experiment in a randomized complete block design with three replications at two experimental sites: Zabol and Iranshahr in two arid locations in southeastern Iran, Sistan and Baluchestan province, Iran. The main plot factor was drought stress, with three irrigation levels: a1 (regional irrigation), a2 (irrigation cessation at 50% flowering stage), and a3 (irrigation cessation at 50% podding stage). The subplot factors were sesame cultivars (Halil, Dashtestan, and Darab) and four zeolite application rates (0, 3, 6, and 9 tons/ha mixed with the soil before sowing). The studied traits included plant height, number of capsules per plant, number of seeds per capsule, 1000-seed weight, seed yield, biological yield, harvest index, and antioxidant enzymes. The highest plant height (111.38 cm) was observed for the Dashtestan cultivar in Zabol under non-stress conditions with 9 tons/ha zeolite application. The highest number of capsules (42.1) and biological yield (3.92 tons/ha) were obtained from Zabol under non-stress conditions with 9 tons/ha zeolite application and the Darab cultivar. The highest number of seeds per capsule (69.96), 1000-seed weight (3.63 g), and seed yield (1.53 tons/ha) were obtained from Iranshahr under non-stress conditions with 9 tons/ha zeolite application and the Halil cultivar. Additionally, the highest activities of catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase were obtained under severe stress conditions (irrigation cessation at 50% flowering stage) without zeolite application in Iranshahr with the Halil cultivar. The highest oil percentage was obtained under non-stress conditions with 9 tons/ha zeolite application and the Darab cultivar in Iranshahr. Zeolite application, particularly at 9 tons/ha, significantly improved seed yield, oil content, and antioxidant enzyme activities under moderate drought (p < 0.05). Among cultivars, Halil demonstrated superior performance under stress conditions, showing higher catalase and peroxidase activity. These findings highlight the potential of using zeolite and drought-tolerant cultivars to enhance sesame productivity in water-limited environments.

干旱胁迫是影响芝麻产量和品质的重要环境因子。本研究采用分裂图析因试验,采用随机完全区组设计,在伊朗东南部锡斯坦和俾路支斯坦省两个干旱地区的Zabol和Iranshahr两个试验点进行3个重复。主要样地因子为干旱胁迫,设置3个灌溉水平:a1(区域灌溉)、a2(50%开花期停止灌溉)和a3(50%结荚期停止灌溉)。分小区因子为芝麻品种(哈利勒、大什斯坦、达拉布)和4种沸石用量(0、3、6、9吨/公顷)。研究的性状包括株高、单株蒴果数、每粒蒴果数、千粒重、种子产量、生物产量、收获指数和抗氧化酶。在无胁迫条件下,投施9吨/公顷沸石时,大什坦的株高最高,达到111.38 cm。Zabol和Darab品种在无胁迫条件下,沸石用量为9吨/公顷,荚果数最高(42.1粒),生物产量最高(3.92吨/公顷)。无胁迫条件下,Iranshahr和Halil品种的每粒种子数最高(69.96粒),千粒重最高(3.63 g),籽粒产量最高(1.53吨/公顷)。此外,在不施用沸石的情况下,在严重胁迫条件下(开花50%时停止灌溉),哈利勒品种的过氧化氢酶、抗坏血酸过氧化物酶、愈创木酚过氧化物酶和多酚氧化酶的活性最高。在无胁迫条件下,使用9吨/公顷沸石和达拉布品种在伊朗沙赫尔获得了最高的含油量。在中度干旱条件下,施用沸石,特别是施用9吨/公顷的沸石,显著提高了种子产量、含油量和抗氧化酶活性(p < 0.05)。在胁迫条件下,哈利尔表现出较强的过氧化氢酶和过氧化物酶活性。这些发现突出了利用沸石和耐旱品种在缺水环境下提高芝麻生产力的潜力。
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引用次数: 0
Spray nozzle type has a small and inconsistent effect on ammonia volatilization from surface-applied urea ammonium-nitrate 喷嘴类型对表面施用尿素-硝酸铵的氨挥发影响较小且不一致
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-11 DOI: 10.1002/agg2.70257
Joshua Nasielski, Michael Gebretsadik Gebre, Sonal Gupta

Ammonia (NH3) volatilization reduces fertilizer use efficiency and increases environmental nitrogen (N) loss when urea ammonium-nitrate (UAN) is surface-applied. Volatilization is driven by localized pH increases resulting from the rapid hydrolysis of large quantities of urea. We hypothesized that nozzles that concentrate UAN in narrow bands would produce greater volatilization than nozzles that broadcast UAN widely. The effect of three nozzle types (dribble band, three-hole streamer, and flat fan) was evaluated in field trials conducted in 2012 and 2013 in Elora, ON, Canada. UAN was surface applied at 168 kg N ha−1 (2012) or 112 kg N ha−1 (2013) onto bare soil multiple times each season. In 2013, UAN was also applied with or without a urease inhibitor (UI) as an additional treatment. Volatilization was measured for 20–34 days after UAN application using the Dosi-Tube method. In 2012, volatilization was overall low (mean = 12.9 kg N ha−1), and nozzle type did not affect volatilization. In 2013, a year with greater overall volatilization even with UI use (mean = 36.3 kg N ha−1), flat fan nozzles reduced NH3 volatilization relative to dribble bands by 22% (p = 0.048). Volatilization from the three-hole streamer nozzle was not different from either nozzle type. The magnitude of the nozzle type effect on volatilization varied across application dates in 2013, likely due to weather variation during the measurement period. The findings suggest that UAN application methods that reduce urea concentration in the soil solution can lead to modest but inconsistent reductions in volatilization.

尿素-硝酸铵(UAN)表面施用时,氨(NH3)挥发降低了肥料利用效率,增加了环境氮(N)损失。挥发是由大量尿素快速水解引起的局部pH值增加所驱动的。我们假设将UAN集中在窄波段的喷嘴会比广泛传播UAN的喷嘴产生更大的挥发。2012年和2013年在加拿大Elora进行了现场试验,评估了三种喷嘴类型(运球带、三孔拖缆和扁平风扇)的效果。每个季节在裸露土壤上以168 kg N ha - 1(2012年)或112 kg N ha - 1(2013年)施用UAN多次。2013年,UAN也作为一种额外的治疗方法,与脲酶抑制剂(UI)联合或不联合使用。使用剂量管法测量UAN应用后20-34天的挥发性。2012年挥发总体较低(平均为12.9 kg N ha−1),喷嘴类型对挥发没有影响。在2013年,即使使用UI,总挥发量也更大(平均= 36.3 kg N ha - 1),扁平风扇喷嘴相对于滴流带减少了22%的NH3挥发(p = 0.048)。三孔飘带喷嘴的挥发性与两种喷嘴的挥发性没有区别。喷嘴类型对挥发的影响程度在2013年不同的应用日期有所不同,可能是由于测量期间的天气变化。研究结果表明,降低土壤溶液中尿素浓度的UAN应用方法可以导致适度但不一致的挥发减少。
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引用次数: 0
Study of morphological diversity, antioxidant capacity, and essential oil compositions of Mentha aquatica L. 水生薄荷的形态多样性、抗氧化能力及精油成分研究。
IF 1.5 Q3 AGRONOMY Pub Date : 2025-11-03 DOI: 10.1002/agg2.70244
Mozhgan Shoghi Jamil, Ali Mehrafarin, Vahid Abdossi, Kambiz Larijani, Raheleh Ebrahimi

Water mint (Mentha aquatica L.) is a medicinal plant with significant therapeutic potential for treating a wide range of diseases. Due to its ability to grow in various geological environments, this study aimed to determine the morphological and phytochemical variability of water mint populations in Northern Iran. Identical and equally sized rhizomes of ten water mint populations were collected from their natural habitats and then cultivated at an experimental farm site in Mazandaran province. The root and stem diameters of most populations were not significantly different. The leaf size varied between populations, with three (Marzonabad, Lafoor, and Hassanabad) exhibiting smaller leaves than the others. The populations with smaller leaf sizes demonstrated a higher leaf number. The highest antioxidant potential was observed in Hassanabad (40 m) and Kia-kola (10 m), with the lowest IC50, while the maximum essentical oil (EO) percentage was found in Balamarznak (250 m). The gas chromatography and gas chromatography/mass spectrometry identified 30 components of EOs, and the main compounds were 1,8-cineol (10.7–27.5%), menthofuran (1.4–17.7%), trans-caryophyllene (1.7–15%), germacrene D, and viridifloro (0–8.08%). Four chemotypes of water mint were distinguished based on biochemical features. Principal component analysis and heat map analysis revealed that leaf size and 1,8-cineol exhibit the minimum variability. However, smart and sensitive morphological traits with the maximum variability were root weight, shoot weight, and the number of leaves and branches. In general, water mint populations with the highest antioxidant capacity and EO content can be obtained within the altitudinal gradient range of 250–300 m.

水薄荷(Mentha aquatica L.)是一种具有治疗多种疾病的显著治疗潜力的药用植物。由于其在各种地质环境中生长的能力,本研究旨在确定伊朗北部水薄荷种群的形态和植物化学变异。从十个水薄荷种群的自然栖息地收集了相同大小的根状茎,然后在马赞达兰省的一个实验农场进行了种植。多数居群的根、茎直径差异不显著。叶片大小因种群而异,其中3个种群(Marzonabad、Lafoor和Hassanabad)的叶片比其他种群小。叶片大小越小的群体叶片数越多。Hassanabad (40 m)和Kia-kola (10 m)的抗氧化潜力最高,IC50最低,Balamarznak (250 m)的精油含量最高。气相色谱和气相色谱/质谱分析鉴定出精油的30个成分,主要化合物为1,8-桉叶油醇(10.7-27.5%)、薄荷呋喃(1.4-17.7%)、反式石竹烯(1.7-15%)、革蕊烯D和绿花素(0-8.08%)。根据水薄荷的生化特征区分出4种化学型。主成分分析和热图分析表明,叶片大小和1,8-桉叶油的变异最小。变异最大的聪明敏感性状是根重、茎重、叶数和分枝数。总体而言,在海拔梯度为250 ~ 300 m范围内,水薄荷种群的抗氧化能力和EO含量最高。
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引用次数: 0
Performance of drought-tolerant maize for silage or grain in conventional and Kura clover perennial groundcover cropping systems 耐旱玉米在传统和库拉三叶草多年生地被作物系统中的青贮或谷物性能
IF 1.5 Q3 AGRONOMY Pub Date : 2025-10-31 DOI: 10.1002/agg2.70255
Kenneth A. Albrecht, Francisco E. Contreras-Govea, Eduardo D. Munaiz, Edward J. Bures, Francisco J. Arriaga

Drought-tolerant (DT) maize (Zea mays L.) hybrids have demonstrated better performance than non-DT hybrids when drought events are present. The objective of this study was to assess the performance of three DT maize hybrids for whole-plant dry matter (DM) and grain yield and forage nutritive value under conventional or Kura clover (Trifolium ambiguum M. Bieb.) perennial groundcover (PGC) cropping systems. This study was conducted for 3 years at two locations, Arlington and Lancaster, WI. Three DT and one non-DT maize hybrid were no-till sown into killed Kura clover (conventional) and living Kura clover that was suppressed with herbicides (PGC). Whole-plant maize, as for silage, was harvested at 50% kernel milk line, and grain was harvested at physiological maturity. Whole-plant biomass was analyzed for forage nutritive value. There was no cropping system × maize hybrid interaction for any of the measured yield or nutritive value parameters. Maize hybrids in the Kura clover PGC system yielded 3.0 Mg ha−1 less whole-plant DM and 1.5 Mg ha−1 less grain than in the conventional system, but whole-plant forage digestibility was slightly greater with the PGC system. DT hybrids yielded slightly less than the non-DT hybrid over both production systems. The DT hybrids demonstrated no advantage relative to a non-DT maize hybrid in a Kura clover PCG system.

在干旱条件下,抗旱玉米(Zea mays L.)杂交品种的表现优于非抗旱玉米杂交品种。本研究旨在评价3个DT玉米杂交种在常规或库拉三叶草(Trifolium ambiguum M. Bieb.)多年生地被(PGC)种植制度下全株干物质(DM)、籽粒产量和饲料营养价值的表现。这项研究在两个地点进行了3年,阿灵顿和兰开斯特,WI。将3个DT玉米杂交种和1个非DT玉米杂交种免耕播种于常规灭活苜蓿和经除草剂抑制的活苜蓿中。整株玉米青贮在籽粒乳线达到50%时收获,籽粒在生理成熟时收获。对全株生物量进行饲料营养价值分析。所有产量和营养价值指标均不存在种植制度与玉米杂交种的交互作用。与常规体系相比,苜蓿PGC体系下玉米杂交种的全株DM和粒数分别减少了3.0 Mg ha−1和1.5 Mg ha−1,但全株牧草消化率略高于常规体系。在两种生产系统中,DT混合动力车的产量略低于非DT混合动力车。在库拉三叶草PCG系统中,DT杂交玉米没有表现出相对于非DT杂交玉米的优势。
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引用次数: 0
Differential expression of the shrunken-2 (sh2) gene in super sweet corn inbred lines under water-limited conditions 水分限制条件下超甜玉米自交系sh2基因的差异表达
IF 1.5 Q3 AGRONOMY Pub Date : 2025-10-31 DOI: 10.1002/agg2.70251
Mohsen Shahrokhi, Saeed Khavari Khorasani, Osler Ortez

Drought stress severely limits corn production, particularly in arid and semi-arid regions, where erratic climate continues to increase the frequency of water scarcity. Super sweet corn (Zea mays L. var. saccharata) is especially vulnerable due to its reduced starch reserves caused by mutations in the shrunken-2 (sh2) gene, which encodes the large subunit of ADP-glucose pyrophosphorylase (AGPase), a key enzyme in starch biosynthesis. This study aimed to evaluate the expression of the sh2 gene under water-limited conditions in two super sweet corn inbred lines recently incorporated into breeding programs: a drought-tolerant line (MCH87002/19-1) and a drought-sensitive line (MCH87004/23-1). The experiment was conducted in 2020 at the Khorasan Razavi Agricultural and Natural Resources Research and Education Center under controlled greenhouse conditions in Mashhad, Iran. Real-time polymerase chain reaction revealed that water-limited stress significantly downregulated sh2 expression in the drought-tolerant line (0.63-fold; p = 0.023), indicating a potential energy-saving strategy under stress. This downregulation was associated with reduced AGPase activity and limited starch biosynthesis. Conversely, sh2 expression in the drought-sensitive line remained unchanged (0.97-fold; p = 0.892), suggesting a lack of transcriptional response to water-limited conditions. These findings highlight the potential of sh2 as a molecular marker for breeding drought-resilient super sweet corn. Integrating sh2 expression profiles into breeding programs could facilitate the development of genotypes that balance sugar content and stress adaptation, contributing to sweet corn productivity in semi-arid environments.

干旱压力严重限制了玉米生产,特别是在干旱和半干旱地区,那里不稳定的气候继续增加缺水的频率。超甜玉米(Zea mays L. var. saccharata)尤其脆弱,因为其淀粉储备减少是由缩水-2 (sh2)基因突变引起的,该基因编码adp -葡萄糖焦磷酸化酶(AGPase)的大亚基,这是淀粉生物合成的关键酶。本研究旨在评估最近纳入育种计划的两个超级甜玉米自交系:耐旱系(MCH87002/19-1)和干旱敏感系(MCH87004/23-1)在限水条件下sh2基因的表达。该实验于2020年在伊朗马什哈德的呼罗珊·拉扎维农业和自然资源研究与教育中心在受控温室条件下进行。实时聚合酶链反应显示,限水胁迫显著下调耐旱系sh2的表达(0.63倍,p = 0.023),表明在胁迫下存在潜在的节能策略。这种下调与AGPase活性降低和淀粉生物合成受限有关。相反,干旱敏感系中sh2的表达保持不变(0.97倍;p = 0.892),表明缺乏对水分限制条件的转录响应。这些发现突出了sh2作为耐旱超甜玉米分子标记的潜力。将sh2表达谱整合到育种计划中可以促进平衡糖含量和逆境适应的基因型的发展,从而有助于半干旱环境下甜玉米的产量。
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引用次数: 0
Genetic profiling of medium-grain Presidio rice mutants for grain quality, grain-filling rate, and abiotic stress tolerance 中粒稻瘟病突变体对籽粒品质、籽粒灌浆速率和非生物抗逆性的遗传分析
IF 1.5 Q3 AGRONOMY Pub Date : 2025-10-30 DOI: 10.1002/agg2.70245
Kimberly S. Ponce, Stanley Omar P. B. Samonte, Darlene L. Sanchez

The demand for medium-grain rice (Oryza sativa L.) in the United States is increasing; therefore, developing high-yielding, high-grain quality, and abiotic stress-tolerant medium-grain rice is crucial to meet the rising demand. In this study, medium-grain rice mutants were developed by irradiating long-grain Presidio rice cultivar. The long-grain Presidio and its 76 sixth-generation (M6) medium-grain mutants were genetically characterized using single-nucleotide polymorphisms (SNPs) associated with 10 traits related to grain quality, grain filling, and abiotic stress tolerance-. Out of the 76 M6 Presidio mutants genotyped, 57 mutants homozygous for the medium-grain allele (GG) of GS3 were selected for further analysis. All mutants and the wild-type Presidio had the OsISA1 allele (Ex17GG) for low chalkiness. The In1T/G, Ex6A/C, and Ex10C/T SNPs of the Wx locus subdivided the mutants into low- and intermediate-amylose classes. Ex10C/T SNP was used to categorize into either soft or hard gel with weak or strong pasting viscosity. The Ex8C/T SNP of SSIIa classified the mutants into two groups (low and intermediate) based on their gelatinization temperatures. Five promising medium-grain Presidio mutants (TIL21271-7-2, TIL21271-7-4, TIL21271-7-5, TIL21271-7-6, and TIL21271-7-8) with high grain filling rate allele of GFR1 and favorable alleles for qSCT1 and OsLPTL159 (cold tolerance), TT1 (heat tolerance), qAG1 and qAG3 (anaerobic germination tolerance), and qDTY1.1, qDTY3.2, and qDTY12.1 (drought tolerance) were identified. This study demonstrates the high potential of mutation breeding in developing mutants with favorable alleles on genes for grain yield, quality, and stress tolerance.

美国对中粒稻(Oryza sativa L.)的需求正在增加;因此,开发高产、优质、非生物抗逆性强的中粒水稻是满足日益增长的需求的关键。本研究利用长粒pressidio水稻品种辐照培育中粒水稻突变体。利用与籽粒品质、籽粒灌浆和非生物抗逆性相关的10个性状相关的单核苷酸多态性(snp)对pressidio及其76个6代(M6)中粒突变体进行了遗传鉴定。从76个M6 Presidio基因分型突变体中,选择了57个GS3中粒等位基因(GG)纯合突变体进行进一步分析。所有突变体和野生型pressidio均含有低垩白的OsISA1等位基因(Ex17GG)。Wx位点的In1T/G、Ex6A/C和Ex10C/T snp将突变体细分为低直链淀粉和中直链淀粉类。用Ex10C/T SNP将凝胶分为软凝胶和硬凝胶,膏体粘度分别为弱和强。siia的Ex8C/T SNP根据其糊化温度将突变体分为两组(低和中)。鉴定出5个具有GFR1高灌浆率等位基因的中粒突变体(TIL21271-7-2、TIL21271-7-4、TIL21271-7-5、TIL21271-7-6和TIL21271-7-8),以及qSCT1和OsLPTL159(耐寒性)、TT1(耐热性)、qAG1和qAG3(厌氧萌发耐受性)和qDTY1.1、qDTY3.2和qDTY12.1(耐旱性)有利等位基因。本研究表明,突变育种在培育具有粮食产量、品质和抗逆性有利等位基因的突变体方面具有很大的潜力。
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Agrosystems, Geosciences & Environment
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