The performance of the cover system, used in the reclamation of a mine site, is primarily assessed through hydraulic properties, including volumetric water content, suction, and saturated hydraulic conductivity (ksat). However, these properties may be influenced by factors such as soil mineralization (which refers to the process by which organic materials are converted into inorganic substances through natural processes), temperature (by the viscosity of water and the activity of microorganisms in the soil), and organic matter content (OMC), which complicate the accurate assessment of cover system performance. To better understand the impact of OMC on hydraulic properties, this study was initiated with two objectives: (i) evaluating the effect of OMC on the water retention curve (WRC) and ksat of a sandy material amended with peat and (ii) proposing equations to predict the WRC of sand amended with organic matter using the Fredlund and Xing model. This was accomplished through laboratory tests that determined the WRC and ksat of sand and sand amended with varying concentrations of peat (0%, 1%, 3%, 5%, 7.5%, 10%, 12.5%, and 15%). The investigation results indicate the air entry value (the suction at which the material begins to desaturate) evaluated using the sand mixture WRC did not show any notable variation. The ksat of the sand mixtures decreases with increasing peat concentration. In terms of prediction, the results obtained for the six mixtures tested in the laboratory showed an excellent agreement between predicted and experimental values, demonstrating the high accuracy with which the WRC s were predicted.
{"title":"Organic matter content influence on the hydraulic properties of sandy material","authors":"Adrien Cabrel Djomo Bouyem, Abdelkabir Maqsoud, Tikou Belem","doi":"10.1002/agg2.70250","DOIUrl":"https://doi.org/10.1002/agg2.70250","url":null,"abstract":"<p>The performance of the cover system, used in the reclamation of a mine site, is primarily assessed through hydraulic properties, including volumetric water content, suction, and saturated hydraulic conductivity (<i>k</i><sub>sat</sub>). However, these properties may be influenced by factors such as soil mineralization (which refers to the process by which organic materials are converted into inorganic substances through natural processes), temperature (by the viscosity of water and the activity of microorganisms in the soil), and organic matter content (OMC), which complicate the accurate assessment of cover system performance. To better understand the impact of OMC on hydraulic properties, this study was initiated with two objectives: (i) evaluating the effect of OMC on the water retention curve (WRC) and <i>k</i><sub>sat</sub> of a sandy material amended with peat and (ii) proposing equations to predict the WRC of sand amended with organic matter using the Fredlund and Xing model. This was accomplished through laboratory tests that determined the WRC and <i>k</i><sub>sat</sub> of sand and sand amended with varying concentrations of peat (0%, 1%, 3%, 5%, 7.5%, 10%, 12.5%, and 15%). The investigation results indicate the air entry value (the suction at which the material begins to desaturate) evaluated using the sand mixture WRC did not show any notable variation. The <i>k</i><sub>sat</sub> of the sand mixtures decreases with increasing peat concentration. In terms of prediction, the results obtained for the six mixtures tested in the laboratory showed an excellent agreement between predicted and experimental values, demonstrating the high accuracy with which the WRC s were predicted.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70250","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145580716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mahnaz Narouei, Alireza Sirousmehr, Mehdi Dahmardeh, Esmaeel Seyedabadi
Drought stress is a significant environmental factor affecting sesame (Sesamum indicum L.) yield and quality. This study was conducted as a split-plot factorial experiment in a randomized complete block design with three replications at two experimental sites: Zabol and Iranshahr in two arid locations in southeastern Iran, Sistan and Baluchestan province, Iran. The main plot factor was drought stress, with three irrigation levels: a1 (regional irrigation), a2 (irrigation cessation at 50% flowering stage), and a3 (irrigation cessation at 50% podding stage). The subplot factors were sesame cultivars (Halil, Dashtestan, and Darab) and four zeolite application rates (0, 3, 6, and 9 tons/ha mixed with the soil before sowing). The studied traits included plant height, number of capsules per plant, number of seeds per capsule, 1000-seed weight, seed yield, biological yield, harvest index, and antioxidant enzymes. The highest plant height (111.38 cm) was observed for the Dashtestan cultivar in Zabol under non-stress conditions with 9 tons/ha zeolite application. The highest number of capsules (42.1) and biological yield (3.92 tons/ha) were obtained from Zabol under non-stress conditions with 9 tons/ha zeolite application and the Darab cultivar. The highest number of seeds per capsule (69.96), 1000-seed weight (3.63 g), and seed yield (1.53 tons/ha) were obtained from Iranshahr under non-stress conditions with 9 tons/ha zeolite application and the Halil cultivar. Additionally, the highest activities of catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase were obtained under severe stress conditions (irrigation cessation at 50% flowering stage) without zeolite application in Iranshahr with the Halil cultivar. The highest oil percentage was obtained under non-stress conditions with 9 tons/ha zeolite application and the Darab cultivar in Iranshahr. Zeolite application, particularly at 9 tons/ha, significantly improved seed yield, oil content, and antioxidant enzyme activities under moderate drought (p < 0.05). Among cultivars, Halil demonstrated superior performance under stress conditions, showing higher catalase and peroxidase activity. These findings highlight the potential of using zeolite and drought-tolerant cultivars to enhance sesame productivity in water-limited environments.
{"title":"Effects of drought stress and zeolite application rates on yield, oil content, and antioxidant enzymes of sesame cultivars in arid regions of Iran","authors":"Mahnaz Narouei, Alireza Sirousmehr, Mehdi Dahmardeh, Esmaeel Seyedabadi","doi":"10.1002/agg2.70256","DOIUrl":"https://doi.org/10.1002/agg2.70256","url":null,"abstract":"<p>Drought stress is a significant environmental factor affecting sesame (<i>Sesamum indicum</i> L.) yield and quality. This study was conducted as a split-plot factorial experiment in a randomized complete block design with three replications at two experimental sites: Zabol and Iranshahr in two arid locations in southeastern Iran, Sistan and Baluchestan province, Iran. The main plot factor was drought stress, with three irrigation levels: a1 (regional irrigation), a2 (irrigation cessation at 50% flowering stage), and a3 (irrigation cessation at 50% podding stage). The subplot factors were sesame cultivars (Halil, Dashtestan, and Darab) and four zeolite application rates (0, 3, 6, and 9 tons/ha mixed with the soil before sowing). The studied traits included plant height, number of capsules per plant, number of seeds per capsule, 1000-seed weight, seed yield, biological yield, harvest index, and antioxidant enzymes. The highest plant height (111.38 cm) was observed for the Dashtestan cultivar in Zabol under non-stress conditions with 9 tons/ha zeolite application. The highest number of capsules (42.1) and biological yield (3.92 tons/ha) were obtained from Zabol under non-stress conditions with 9 tons/ha zeolite application and the Darab cultivar. The highest number of seeds per capsule (69.96), 1000-seed weight (3.63 g), and seed yield (1.53 tons/ha) were obtained from Iranshahr under non-stress conditions with 9 tons/ha zeolite application and the Halil cultivar. Additionally, the highest activities of catalase, ascorbate peroxidase, guaiacol peroxidase, and polyphenol oxidase were obtained under severe stress conditions (irrigation cessation at 50% flowering stage) without zeolite application in Iranshahr with the Halil cultivar. The highest oil percentage was obtained under non-stress conditions with 9 tons/ha zeolite application and the Darab cultivar in Iranshahr. Zeolite application, particularly at 9 tons/ha, significantly improved seed yield, oil content, and antioxidant enzyme activities under moderate drought (<i>p</i> < 0.05). Among cultivars, Halil demonstrated superior performance under stress conditions, showing higher catalase and peroxidase activity. These findings highlight the potential of using zeolite and drought-tolerant cultivars to enhance sesame productivity in water-limited environments.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145522134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joshua Nasielski, Michael Gebretsadik Gebre, Sonal Gupta
Ammonia (NH3) volatilization reduces fertilizer use efficiency and increases environmental nitrogen (N) loss when urea ammonium-nitrate (UAN) is surface-applied. Volatilization is driven by localized pH increases resulting from the rapid hydrolysis of large quantities of urea. We hypothesized that nozzles that concentrate UAN in narrow bands would produce greater volatilization than nozzles that broadcast UAN widely. The effect of three nozzle types (dribble band, three-hole streamer, and flat fan) was evaluated in field trials conducted in 2012 and 2013 in Elora, ON, Canada. UAN was surface applied at 168 kg N ha−1 (2012) or 112 kg N ha−1 (2013) onto bare soil multiple times each season. In 2013, UAN was also applied with or without a urease inhibitor (UI) as an additional treatment. Volatilization was measured for 20–34 days after UAN application using the Dosi-Tube method. In 2012, volatilization was overall low (mean = 12.9 kg N ha−1), and nozzle type did not affect volatilization. In 2013, a year with greater overall volatilization even with UI use (mean = 36.3 kg N ha−1), flat fan nozzles reduced NH3 volatilization relative to dribble bands by 22% (p = 0.048). Volatilization from the three-hole streamer nozzle was not different from either nozzle type. The magnitude of the nozzle type effect on volatilization varied across application dates in 2013, likely due to weather variation during the measurement period. The findings suggest that UAN application methods that reduce urea concentration in the soil solution can lead to modest but inconsistent reductions in volatilization.
尿素-硝酸铵(UAN)表面施用时,氨(NH3)挥发降低了肥料利用效率,增加了环境氮(N)损失。挥发是由大量尿素快速水解引起的局部pH值增加所驱动的。我们假设将UAN集中在窄波段的喷嘴会比广泛传播UAN的喷嘴产生更大的挥发。2012年和2013年在加拿大Elora进行了现场试验,评估了三种喷嘴类型(运球带、三孔拖缆和扁平风扇)的效果。每个季节在裸露土壤上以168 kg N ha - 1(2012年)或112 kg N ha - 1(2013年)施用UAN多次。2013年,UAN也作为一种额外的治疗方法,与脲酶抑制剂(UI)联合或不联合使用。使用剂量管法测量UAN应用后20-34天的挥发性。2012年挥发总体较低(平均为12.9 kg N ha−1),喷嘴类型对挥发没有影响。在2013年,即使使用UI,总挥发量也更大(平均= 36.3 kg N ha - 1),扁平风扇喷嘴相对于滴流带减少了22%的NH3挥发(p = 0.048)。三孔飘带喷嘴的挥发性与两种喷嘴的挥发性没有区别。喷嘴类型对挥发的影响程度在2013年不同的应用日期有所不同,可能是由于测量期间的天气变化。研究结果表明,降低土壤溶液中尿素浓度的UAN应用方法可以导致适度但不一致的挥发减少。
{"title":"Spray nozzle type has a small and inconsistent effect on ammonia volatilization from surface-applied urea ammonium-nitrate","authors":"Joshua Nasielski, Michael Gebretsadik Gebre, Sonal Gupta","doi":"10.1002/agg2.70257","DOIUrl":"https://doi.org/10.1002/agg2.70257","url":null,"abstract":"<p>Ammonia (NH<sub>3</sub>) volatilization reduces fertilizer use efficiency and increases environmental nitrogen (N) loss when urea ammonium-nitrate (UAN) is surface-applied. Volatilization is driven by localized pH increases resulting from the rapid hydrolysis of large quantities of urea. We hypothesized that nozzles that concentrate UAN in narrow bands would produce greater volatilization than nozzles that broadcast UAN widely. The effect of three nozzle types (dribble band, three-hole streamer, and flat fan) was evaluated in field trials conducted in 2012 and 2013 in Elora, ON, Canada. UAN was surface applied at 168 kg N ha<sup>−1</sup> (2012) or 112 kg N ha<sup>−1</sup> (2013) onto bare soil multiple times each season. In 2013, UAN was also applied with or without a urease inhibitor (UI) as an additional treatment. Volatilization was measured for 20–34 days after UAN application using the Dosi-Tube method. In 2012, volatilization was overall low (mean = 12.9 kg N ha<sup>−1</sup>), and nozzle type did not affect volatilization. In 2013, a year with greater overall volatilization even with UI use (mean = 36.3 kg N ha<sup>−1</sup>), flat fan nozzles reduced NH<sub>3</sub> volatilization relative to dribble bands by 22% (<i>p </i>= 0.048). Volatilization from the three-hole streamer nozzle was not different from either nozzle type. The magnitude of the nozzle type effect on volatilization varied across application dates in 2013, likely due to weather variation during the measurement period. The findings suggest that UAN application methods that reduce urea concentration in the soil solution can lead to modest but inconsistent reductions in volatilization.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70257","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145521726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mozhgan Shoghi Jamil, Ali Mehrafarin, Vahid Abdossi, Kambiz Larijani, Raheleh Ebrahimi
Water mint (Mentha aquatica L.) is a medicinal plant with significant therapeutic potential for treating a wide range of diseases. Due to its ability to grow in various geological environments, this study aimed to determine the morphological and phytochemical variability of water mint populations in Northern Iran. Identical and equally sized rhizomes of ten water mint populations were collected from their natural habitats and then cultivated at an experimental farm site in Mazandaran province. The root and stem diameters of most populations were not significantly different. The leaf size varied between populations, with three (Marzonabad, Lafoor, and Hassanabad) exhibiting smaller leaves than the others. The populations with smaller leaf sizes demonstrated a higher leaf number. The highest antioxidant potential was observed in Hassanabad (40 m) and Kia-kola (10 m), with the lowest IC50, while the maximum essentical oil (EO) percentage was found in Balamarznak (250 m). The gas chromatography and gas chromatography/mass spectrometry identified 30 components of EOs, and the main compounds were 1,8-cineol (10.7–27.5%), menthofuran (1.4–17.7%), trans-caryophyllene (1.7–15%), germacrene D, and viridifloro (0–8.08%). Four chemotypes of water mint were distinguished based on biochemical features. Principal component analysis and heat map analysis revealed that leaf size and 1,8-cineol exhibit the minimum variability. However, smart and sensitive morphological traits with the maximum variability were root weight, shoot weight, and the number of leaves and branches. In general, water mint populations with the highest antioxidant capacity and EO content can be obtained within the altitudinal gradient range of 250–300 m.
{"title":"Study of morphological diversity, antioxidant capacity, and essential oil compositions of Mentha aquatica L.","authors":"Mozhgan Shoghi Jamil, Ali Mehrafarin, Vahid Abdossi, Kambiz Larijani, Raheleh Ebrahimi","doi":"10.1002/agg2.70244","DOIUrl":"https://doi.org/10.1002/agg2.70244","url":null,"abstract":"<p>Water mint (<i>Mentha aquatica</i> L.) is a medicinal plant with significant therapeutic potential for treating a wide range of diseases. Due to its ability to grow in various geological environments, this study aimed to determine the morphological and phytochemical variability of water mint populations in Northern Iran. Identical and equally sized rhizomes of ten water mint populations were collected from their natural habitats and then cultivated at an experimental farm site in Mazandaran province. The root and stem diameters of most populations were not significantly different. The leaf size varied between populations, with three (Marzonabad, Lafoor, and Hassanabad) exhibiting smaller leaves than the others. The populations with smaller leaf sizes demonstrated a higher leaf number. The highest antioxidant potential was observed in Hassanabad (40 m) and Kia-kola (10 m), with the lowest IC50, while the maximum essentical oil (EO) percentage was found in Balamarznak (250 m). The gas chromatography and gas chromatography/mass spectrometry identified 30 components of EOs, and the main compounds were 1,8-cineol (10.7–27.5%), menthofuran (1.4–17.7%), <i>trans</i>-caryophyllene (1.7–15%), germacrene D, and viridifloro (0–8.08%). Four chemotypes of water mint were distinguished based on biochemical features. Principal component analysis and heat map analysis revealed that leaf size and 1,8-cineol exhibit the minimum variability. However, smart and sensitive morphological traits with the maximum variability were root weight, shoot weight, and the number of leaves and branches. In general, water mint populations with the highest antioxidant capacity and EO content can be obtained within the altitudinal gradient range of 250–300 m.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145469559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenneth A. Albrecht, Francisco E. Contreras-Govea, Eduardo D. Munaiz, Edward J. Bures, Francisco J. Arriaga
Drought-tolerant (DT) maize (Zea mays L.) hybrids have demonstrated better performance than non-DT hybrids when drought events are present. The objective of this study was to assess the performance of three DT maize hybrids for whole-plant dry matter (DM) and grain yield and forage nutritive value under conventional or Kura clover (Trifolium ambiguum M. Bieb.) perennial groundcover (PGC) cropping systems. This study was conducted for 3 years at two locations, Arlington and Lancaster, WI. Three DT and one non-DT maize hybrid were no-till sown into killed Kura clover (conventional) and living Kura clover that was suppressed with herbicides (PGC). Whole-plant maize, as for silage, was harvested at 50% kernel milk line, and grain was harvested at physiological maturity. Whole-plant biomass was analyzed for forage nutritive value. There was no cropping system × maize hybrid interaction for any of the measured yield or nutritive value parameters. Maize hybrids in the Kura clover PGC system yielded 3.0 Mg ha−1 less whole-plant DM and 1.5 Mg ha−1 less grain than in the conventional system, but whole-plant forage digestibility was slightly greater with the PGC system. DT hybrids yielded slightly less than the non-DT hybrid over both production systems. The DT hybrids demonstrated no advantage relative to a non-DT maize hybrid in a Kura clover PCG system.
在干旱条件下,抗旱玉米(Zea mays L.)杂交品种的表现优于非抗旱玉米杂交品种。本研究旨在评价3个DT玉米杂交种在常规或库拉三叶草(Trifolium ambiguum M. Bieb.)多年生地被(PGC)种植制度下全株干物质(DM)、籽粒产量和饲料营养价值的表现。这项研究在两个地点进行了3年,阿灵顿和兰开斯特,WI。将3个DT玉米杂交种和1个非DT玉米杂交种免耕播种于常规灭活苜蓿和经除草剂抑制的活苜蓿中。整株玉米青贮在籽粒乳线达到50%时收获,籽粒在生理成熟时收获。对全株生物量进行饲料营养价值分析。所有产量和营养价值指标均不存在种植制度与玉米杂交种的交互作用。与常规体系相比,苜蓿PGC体系下玉米杂交种的全株DM和粒数分别减少了3.0 Mg ha−1和1.5 Mg ha−1,但全株牧草消化率略高于常规体系。在两种生产系统中,DT混合动力车的产量略低于非DT混合动力车。在库拉三叶草PCG系统中,DT杂交玉米没有表现出相对于非DT杂交玉米的优势。
{"title":"Performance of drought-tolerant maize for silage or grain in conventional and Kura clover perennial groundcover cropping systems","authors":"Kenneth A. Albrecht, Francisco E. Contreras-Govea, Eduardo D. Munaiz, Edward J. Bures, Francisco J. Arriaga","doi":"10.1002/agg2.70255","DOIUrl":"https://doi.org/10.1002/agg2.70255","url":null,"abstract":"<p>Drought-tolerant (DT) maize (<i>Zea mays</i> L.) hybrids have demonstrated better performance than non-DT hybrids when drought events are present. The objective of this study was to assess the performance of three DT maize hybrids for whole-plant dry matter (DM) and grain yield and forage nutritive value under conventional or Kura clover (<i>Trifolium ambiguum</i> M. Bieb.) perennial groundcover (PGC) cropping systems. This study was conducted for 3 years at two locations, Arlington and Lancaster, WI. Three DT and one non-DT maize hybrid were no-till sown into killed Kura clover (conventional) and living Kura clover that was suppressed with herbicides (PGC). Whole-plant maize, as for silage, was harvested at 50% kernel milk line, and grain was harvested at physiological maturity. Whole-plant biomass was analyzed for forage nutritive value. There was no cropping system × maize hybrid interaction for any of the measured yield or nutritive value parameters. Maize hybrids in the Kura clover PGC system yielded 3.0 Mg ha<sup>−1</sup> less whole-plant DM and 1.5 Mg ha<sup>−1</sup> less grain than in the conventional system, but whole-plant forage digestibility was slightly greater with the PGC system. DT hybrids yielded slightly less than the non-DT hybrid over both production systems. The DT hybrids demonstrated no advantage relative to a non-DT maize hybrid in a Kura clover PCG system.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70255","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Drought stress severely limits corn production, particularly in arid and semi-arid regions, where erratic climate continues to increase the frequency of water scarcity. Super sweet corn (Zea mays L. var. saccharata) is especially vulnerable due to its reduced starch reserves caused by mutations in the shrunken-2 (sh2) gene, which encodes the large subunit of ADP-glucose pyrophosphorylase (AGPase), a key enzyme in starch biosynthesis. This study aimed to evaluate the expression of the sh2 gene under water-limited conditions in two super sweet corn inbred lines recently incorporated into breeding programs: a drought-tolerant line (MCH87002/19-1) and a drought-sensitive line (MCH87004/23-1). The experiment was conducted in 2020 at the Khorasan Razavi Agricultural and Natural Resources Research and Education Center under controlled greenhouse conditions in Mashhad, Iran. Real-time polymerase chain reaction revealed that water-limited stress significantly downregulated sh2 expression in the drought-tolerant line (0.63-fold; p = 0.023), indicating a potential energy-saving strategy under stress. This downregulation was associated with reduced AGPase activity and limited starch biosynthesis. Conversely, sh2 expression in the drought-sensitive line remained unchanged (0.97-fold; p = 0.892), suggesting a lack of transcriptional response to water-limited conditions. These findings highlight the potential of sh2 as a molecular marker for breeding drought-resilient super sweet corn. Integrating sh2 expression profiles into breeding programs could facilitate the development of genotypes that balance sugar content and stress adaptation, contributing to sweet corn productivity in semi-arid environments.
干旱压力严重限制了玉米生产,特别是在干旱和半干旱地区,那里不稳定的气候继续增加缺水的频率。超甜玉米(Zea mays L. var. saccharata)尤其脆弱,因为其淀粉储备减少是由缩水-2 (sh2)基因突变引起的,该基因编码adp -葡萄糖焦磷酸化酶(AGPase)的大亚基,这是淀粉生物合成的关键酶。本研究旨在评估最近纳入育种计划的两个超级甜玉米自交系:耐旱系(MCH87002/19-1)和干旱敏感系(MCH87004/23-1)在限水条件下sh2基因的表达。该实验于2020年在伊朗马什哈德的呼罗珊·拉扎维农业和自然资源研究与教育中心在受控温室条件下进行。实时聚合酶链反应显示,限水胁迫显著下调耐旱系sh2的表达(0.63倍,p = 0.023),表明在胁迫下存在潜在的节能策略。这种下调与AGPase活性降低和淀粉生物合成受限有关。相反,干旱敏感系中sh2的表达保持不变(0.97倍;p = 0.892),表明缺乏对水分限制条件的转录响应。这些发现突出了sh2作为耐旱超甜玉米分子标记的潜力。将sh2表达谱整合到育种计划中可以促进平衡糖含量和逆境适应的基因型的发展,从而有助于半干旱环境下甜玉米的产量。
{"title":"Differential expression of the shrunken-2 (sh2) gene in super sweet corn inbred lines under water-limited conditions","authors":"Mohsen Shahrokhi, Saeed Khavari Khorasani, Osler Ortez","doi":"10.1002/agg2.70251","DOIUrl":"https://doi.org/10.1002/agg2.70251","url":null,"abstract":"<p>Drought stress severely limits corn production, particularly in arid and semi-arid regions, where erratic climate continues to increase the frequency of water scarcity. Super sweet corn (<i>Zea mays</i> L. var. <i>saccharata</i>) is especially vulnerable due to its reduced starch reserves caused by mutations in the <i>shrunken-2</i> (<i>sh2</i>) gene, which encodes the large subunit of ADP-glucose pyrophosphorylase (AGPase), a key enzyme in starch biosynthesis. This study aimed to evaluate the expression of the <i>sh2</i> gene under water-limited conditions in two super sweet corn inbred lines recently incorporated into breeding programs: a drought-tolerant line (MCH87002/19-1) and a drought-sensitive line (MCH87004/23-1). The experiment was conducted in 2020 at the Khorasan Razavi Agricultural and Natural Resources Research and Education Center under controlled greenhouse conditions in Mashhad, Iran. Real-time polymerase chain reaction revealed that water-limited stress significantly downregulated <i>sh2</i> expression in the drought-tolerant line (0.63-fold; <i>p</i> = 0.023), indicating a potential energy-saving strategy under stress. This downregulation was associated with reduced AGPase activity and limited starch biosynthesis. Conversely, <i>sh2</i> expression in the drought-sensitive line remained unchanged (0.97-fold; <i>p</i> = 0.892), suggesting a lack of transcriptional response to water-limited conditions. These findings highlight the potential of <i>sh2</i> as a molecular marker for breeding drought-resilient super sweet corn. Integrating <i>sh2</i> expression profiles into breeding programs could facilitate the development of genotypes that balance sugar content and stress adaptation, contributing to sweet corn productivity in semi-arid environments.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kimberly S. Ponce, Stanley Omar P. B. Samonte, Darlene L. Sanchez
The demand for medium-grain rice (Oryza sativa L.) in the United States is increasing; therefore, developing high-yielding, high-grain quality, and abiotic stress-tolerant medium-grain rice is crucial to meet the rising demand. In this study, medium-grain rice mutants were developed by irradiating long-grain Presidio rice cultivar. The long-grain Presidio and its 76 sixth-generation (M6) medium-grain mutants were genetically characterized using single-nucleotide polymorphisms (SNPs) associated with 10 traits related to grain quality, grain filling, and abiotic stress tolerance-. Out of the 76 M6 Presidio mutants genotyped, 57 mutants homozygous for the medium-grain allele (GG) of GS3 were selected for further analysis. All mutants and the wild-type Presidio had the OsISA1 allele (Ex17GG) for low chalkiness. The In1T/G, Ex6A/C, and Ex10C/T SNPs of the Wx locus subdivided the mutants into low- and intermediate-amylose classes. Ex10C/T SNP was used to categorize into either soft or hard gel with weak or strong pasting viscosity. The Ex8C/T SNP of SSIIa classified the mutants into two groups (low and intermediate) based on their gelatinization temperatures. Five promising medium-grain Presidio mutants (TIL21271-7-2, TIL21271-7-4, TIL21271-7-5, TIL21271-7-6, and TIL21271-7-8) with high grain filling rate allele of GFR1 and favorable alleles for qSCT1 and OsLPTL159 (cold tolerance), TT1 (heat tolerance), qAG1 and qAG3 (anaerobic germination tolerance), and qDTY1.1, qDTY3.2, and qDTY12.1 (drought tolerance) were identified. This study demonstrates the high potential of mutation breeding in developing mutants with favorable alleles on genes for grain yield, quality, and stress tolerance.
{"title":"Genetic profiling of medium-grain Presidio rice mutants for grain quality, grain-filling rate, and abiotic stress tolerance","authors":"Kimberly S. Ponce, Stanley Omar P. B. Samonte, Darlene L. Sanchez","doi":"10.1002/agg2.70245","DOIUrl":"https://doi.org/10.1002/agg2.70245","url":null,"abstract":"<p>The demand for medium-grain rice (<i>Oryza sativa</i> L.) in the United States is increasing; therefore, developing high-yielding, high-grain quality, and abiotic stress-tolerant medium-grain rice is crucial to meet the rising demand. In this study, medium-grain rice mutants were developed by irradiating long-grain Presidio rice cultivar. The long-grain Presidio and its 76 sixth-generation (M<sub>6</sub>) medium-grain mutants were genetically characterized using single-nucleotide polymorphisms (SNPs) associated with 10 traits related to grain quality, grain filling, and abiotic stress tolerance-. Out of the 76 M<sub>6</sub> Presidio mutants genotyped, 57 mutants homozygous for the medium-grain allele (GG) of GS3 were selected for further analysis. All mutants and the wild-type Presidio had the <i>OsISA1</i> allele (Ex17GG) for low chalkiness. The In1T/G, Ex6A/C, and Ex10C/T SNPs of the <i>Wx</i> locus subdivided the mutants into low- and intermediate-amylose classes. Ex10C/T SNP was used to categorize into either soft or hard gel with weak or strong pasting viscosity. The Ex8C/T SNP of <i>SSIIa</i> classified the mutants into two groups (low and intermediate) based on their gelatinization temperatures. Five promising medium-grain Presidio mutants (TIL21271-7-2, TIL21271-7-4, TIL21271-7-5, TIL21271-7-6, and TIL21271-7-8) with high grain filling rate allele of <i>GFR1</i> and favorable alleles for <i>qSCT1</i> and <i>OsLPTL159</i> (cold tolerance), <i>TT1</i> (heat tolerance), <i>qAG1</i> and <i>qAG3</i> (anaerobic germination tolerance), and <i>qDTY1.1</i>, <i>qDTY3.2</i>, and <i>qDTY12.1</i> (drought tolerance) were identified. This study demonstrates the high potential of mutation breeding in developing mutants with favorable alleles on genes for grain yield, quality, and stress tolerance.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70245","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Harsanjam Singh, Josh Lofton, Daryl Brian Arnall, Alex Rocateli, Sumit Sharma
With declining of the Ogallala Aquifer in the Central High Plains, cotton (Gossypium hirsutum L.) has emerged as a low-water-demanding alternative to corn (Zea mays). However, cotton harvest leaves minimal cover on the soil surface following harvest due to application of harvest aids. Planting cover crops following cotton harvest is not always viable as it often leaves insufficient time for establishing covers due to cold and dry conditions in the region. Seeding cover crops in standing cotton may be a viable option; however, the effect of harvest aids on these covers remains unknown. This study evaluates the response of cover crops winter pea (Pisum sativum), triticale (Triticosecale rimpaui Wittm), hairy vetch (Vicia villosa), black oats (Avena sativa), crimson clover (Trifolium incarnatum), and rapeseed (Brassica napus L.) to different cotton harvest aids (DFT-6EC and AIM). The crops were grown in pots in climate-controlled chambers and green cover was measured weekly using Canopeo application. The crops were sprayed with harvest aids 42 days after planting. The green cover was reduced in all crops by at least 45% within 2 weeks after spraying for both harvest aids when compared to check pots, with winter pea losing 100% of green cover. The average harvest biomass was reduced by 45% and 52% in pots sprayed with AIM and DFT as compared to check pots, respectively. These results show that cover crops are susceptible to cotton harvest aids and will need adequate time to produce biomass before harvest aid application if interseeded in cotton crops.
{"title":"Winter cover crops’ response to different cotton harvest aids","authors":"Harsanjam Singh, Josh Lofton, Daryl Brian Arnall, Alex Rocateli, Sumit Sharma","doi":"10.1002/agg2.70246","DOIUrl":"https://doi.org/10.1002/agg2.70246","url":null,"abstract":"<p>With declining of the Ogallala Aquifer in the Central High Plains, cotton (<i>Gossypium hirsutum</i> L.) has emerged as a low-water-demanding alternative to corn (<i>Zea mays</i>). However, cotton harvest leaves minimal cover on the soil surface following harvest due to application of harvest aids. Planting cover crops following cotton harvest is not always viable as it often leaves insufficient time for establishing covers due to cold and dry conditions in the region. Seeding cover crops in standing cotton may be a viable option; however, the effect of harvest aids on these covers remains unknown. This study evaluates the response of cover crops winter pea (<i>Pisum sativum</i>), triticale (<i>Triticosecale rimpaui Wittm</i>), hairy vetch (<i>Vicia villosa</i>), black oats (<i>Avena sativa</i>), crimson clover (<i>Trifolium incarnatum</i>), and rapeseed (<i>Brassica napus</i> L.) to different cotton harvest aids (DFT-6EC and AIM). The crops were grown in pots in climate-controlled chambers and green cover was measured weekly using Canopeo application. The crops were sprayed with harvest aids 42 days after planting. The green cover was reduced in all crops by at least 45% within 2 weeks after spraying for both harvest aids when compared to check pots, with winter pea losing 100% of green cover. The average harvest biomass was reduced by 45% and 52% in pots sprayed with AIM and DFT as compared to check pots, respectively. These results show that cover crops are susceptible to cotton harvest aids and will need adequate time to produce biomass before harvest aid application if interseeded in cotton crops.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70246","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. V Pessotto, T. L. Roberts, C. dos Santos, K. A. Hoegenauer, M. Bertucci, W. J. Ross, M. Savin
Cover cropping is an important management practice that can benefit the cash crop and the environment. Plant species, weather, and cover crop termination influence biomass production and N fixation potential. This study was conducted to determine optimal termination dates for production systems in Arkansas based on growing degree days (GDD) for eight different cover crop species: Austrian winter pea (AWP) (Pisum sativum), balansa clover (Trifolium michelianum), barley (Hordeum vulgare), black-seeded oats (Avena sativa), common vetch (Vicia sativa var. Cahaba), cereal rye (Secale cereale), crimson clover (Trifolium incarnatum), and hairy vetch (Vicia villosa). Field studies were conducted at three research stations in Arkansas to provide differences in climate and rate of GDD accumulation. An area of 0.17 m2 was harvested for aboveground biomass and total N uptake randomly within each experimental unit every 2 weeks, starting on February 15. Aboveground biomass accumulation and N content were regressed as a function of GDD for each cover crop treatment. At the Rohwer site, AWP, for example, accumulated an average of 3643 kg ha−1 of biomass and 107 kg N ha−1 at termination, whereas 30 days before termination, the average was 1868 kg ha−1 of biomass and 69 kg N ha−1. The growth rate increased dramatically closer to termination due to warmer temperatures, which allowed for rapid GDD and aboveground biomass accumulation. These results suggest that termination dates can be identified using previous and forecasted weather data.
覆盖种植是一项重要的经济作物和环境效益管理措施。植物种类、天气和覆盖作物终止影响生物量生产和固氮潜力。本研究基于生长度天数(GDD)确定阿肯色州8种不同覆盖作物的生产系统的最佳终止日期:奥地利冬豌豆(AWP)、三叶草(Trifolium michelianum)、大麦(Hordeum vulgare)、黑籽燕麦(Avena sativa)、普通豇豆(Vicia sativa var. Cahaba)、谷物黑麦(Secale cereale)、深红色三叶草(Trifolium incarnatum)和毛豌豆(Vicia villosa)。在阿肯色州的三个研究站进行了实地研究,以提供气候和GDD积累速度的差异。从2月15日开始,每个试验单元每2周随机收获地上生物量和总氮吸收量0.17 m2。对不同覆盖作物处理的地上生物量积累和氮素含量进行了GDD函数回归。例如,在Rohwer站点,AWP在终止时平均积累了3643 kg ha - 1生物量和107 kg N ha - 1,而在终止前30天,平均积累了1868 kg ha - 1生物量和69 kg N ha - 1。由于温度升高,生长速度在接近终止时急剧增加,这允许快速的GDD和地上生物量积累。这些结果表明,可以使用以前和预测的天气数据来确定终止日期。
{"title":"Use of growing degree days to model the dynamics of aboveground biomass and nitrogen accumulation of winter cover crops","authors":"M. V Pessotto, T. L. Roberts, C. dos Santos, K. A. Hoegenauer, M. Bertucci, W. J. Ross, M. Savin","doi":"10.1002/agg2.70249","DOIUrl":"https://doi.org/10.1002/agg2.70249","url":null,"abstract":"<p>Cover cropping is an important management practice that can benefit the cash crop and the environment. Plant species, weather, and cover crop termination influence biomass production and N fixation potential. This study was conducted to determine optimal termination dates for production systems in Arkansas based on growing degree days (GDD) for eight different cover crop species: Austrian winter pea (AWP) (<i>Pisum sativum</i>), balansa clover (<i>Trifolium michelianum</i>), barley (<i>Hordeum vulgare</i>), black-seeded oats (<i>Avena sativa</i>), common vetch (<i>Vicia sativa</i> var. Cahaba), cereal rye (<i>Secale cereale</i>), crimson clover (<i>Trifolium incarnatum</i>), and hairy vetch (<i>Vicia villosa</i>). Field studies were conducted at three research stations in Arkansas to provide differences in climate and rate of GDD accumulation. An area of 0.17 m<sup>2</sup> was harvested for aboveground biomass and total N uptake randomly within each experimental unit every 2 weeks, starting on February 15. Aboveground biomass accumulation and N content were regressed as a function of GDD for each cover crop treatment. At the Rohwer site, AWP, for example, accumulated an average of 3643 kg ha<sup>−1</sup> of biomass and 107 kg N ha<sup>−1</sup> at termination, whereas 30 days before termination, the average was 1868 kg ha<sup>−1</sup> of biomass and 69 kg N ha<sup>−1</sup>. The growth rate increased dramatically closer to termination due to warmer temperatures, which allowed for rapid GDD and aboveground biomass accumulation. These results suggest that termination dates can be identified using previous and forecasted weather data.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soil fertility depletion, particularly nitrogen and phosphorus, is a major constraint to wheat (Triticum aestivum L.) productivity in Ethiopia, influencing crop yields and food security. A field experiment was conducted to determine the optimum nitrogen and phosphorus rates for wheat yield, nutrient uptake, nutrient use efficiency, and economic returns in Wadla District, North Wollo, Ethiopia. The experiment was arranged in a factorial randomized complete block design with three replications. The treatment consisted of 46, 92, 138, and 184 kg ha−1 N levels and 23, 46, 69, and 92 kg ha−1 P2O5 levels. Yield and yield component parameters, as well as a plant sample, were collected. The collected data were analyzed using Statistical Application Software in a mixed model. The results showed that the nutrient levels significantly influenced yield, yield components, nutrient uptake, nutrient use efficiency, and economic returns. The maximum biological grain (3840.0 kg ha−1) and biomass (7910 kg ha−1) yields were recorded at 138 kg N ha−1 with 92 kg P2O5 ha−1. The highest performance, in terms of economically yield-related parameters, was recorded with the combined application of 138 kg N ha−1 and 69 kg P2O5 ha−1, resulting in biomass and grain yields of 7800 and 3770 kg ha−1, respectively. Total nitrogen uptake was high for 138 kg N ha−1 (82.52 kg N ha−1), while phosphorus uptake was high for 69 kg P2O5 ha−1 (18.42 kg P ha−1), with uptake efficiency decreasing at higher rates. The nutrient use efficiency parameter also reached its highest values at the lowest to moderate N and P levels. Partial budget analysis indicates that the applications of 138 kg N ha−1 with 69 kg P2O5 ha−1 gave the highest net benefit of 258,954.2 ETB ha−1, with a marginal return of 5825.3%. Therefore, the application of 138 kg N ha−1 and 69 kg P2O5 ha−1 is recommended for maximizing wheat yield, economic returns, and enhancing nutrient uptake and nutrient efficiency in Wadla and similar agro-ecological areas. Further research should be done on the long-term residual effect of P and nutrient efficiency through regular soil testing and monitoring to enhance nutrient uptake while minimizing environmental footprints.
土壤肥力枯竭,特别是氮和磷的枯竭,是埃塞俄比亚小麦(Triticum aestivum L.)生产力的主要制约因素,影响作物产量和粮食安全。在埃塞俄比亚北沃罗的Wadla地区进行了田间试验,以确定氮磷素对小麦产量、养分吸收、养分利用效率和经济效益的最佳影响。试验采用因子随机完全区组设计,设3个重复。处理包括46、92、138和184 kg ha−1 N水平和23、46、69和92 kg ha−1 P2O5水平。收集了产量和产量组成参数以及植株样品。采用统计应用软件对收集的数据进行混合模型分析。结果表明,不同营养水平对水稻产量、产量构成、养分吸收、养分利用效率和经济效益均有显著影响。最大生物粒(3840.0 kg ha - 1)和生物量(7910 kg ha - 1)产量记录为138 kg N ha - 1和92 kg P2O5 ha - 1。就经济产量相关参数而言,138 kg N + 69 kg P2O5组合施用的产量最高,生物量和粮食产量分别达到7800 kg ha - 1和3770 kg ha - 1。总氮吸收量在138 kg N ha−1 (82.52 kg N ha−1)时较高,而磷吸收量在69 kg P2O5 ha−1 (18.42 kg P ha−1)时较高,且吸收量随速率的增加而降低。在低、中氮磷水平下,养分利用效率参数达到最大值。部分预算分析表明,施用138 kg N ha - 1和69 kg P2O5 ha - 1的净效益最高,为258,954.2 ETB ha - 1,边际回报率为5825.3%。因此,在Wadla和类似的农业生态区,建议施用138 kg N和69 kg P2O5 ha - 1,以最大限度地提高小麦产量和经济效益,提高养分吸收和养分效率。通过定期的土壤测试和监测,进一步研究磷的长期残留效应和养分效率,以提高养分吸收,同时最大限度地减少环境足迹。
{"title":"Optimizing bread wheat (Triticum aestivum L.) yield and nutrient use efficiency through nitrogen and phosphorus management in Nitisols of Eastern Amhara Highlands, Ethiopia","authors":"Kassa Sisay, Yihenew G. Selassie, Birru Yitaferu","doi":"10.1002/agg2.70248","DOIUrl":"https://doi.org/10.1002/agg2.70248","url":null,"abstract":"<p>Soil fertility depletion, particularly nitrogen and phosphorus, is a major constraint to wheat (<i>Triticum aestivum</i> L.) productivity in Ethiopia, influencing crop yields and food security. A field experiment was conducted to determine the optimum nitrogen and phosphorus rates for wheat yield, nutrient uptake, nutrient use efficiency, and economic returns in Wadla District, North Wollo, Ethiopia. The experiment was arranged in a factorial randomized complete block design with three replications. The treatment consisted of 46, 92, 138, and 184 kg ha<sup>−1</sup> N levels and 23, 46, 69, and 92 kg ha<sup>−1</sup> P<sub>2</sub>O<sub>5</sub> levels. Yield and yield component parameters, as well as a plant sample, were collected. The collected data were analyzed using Statistical Application Software in a mixed model. The results showed that the nutrient levels significantly influenced yield, yield components, nutrient uptake, nutrient use efficiency, and economic returns. The maximum biological grain (3840.0 kg ha<sup>−1</sup>) and biomass (7910 kg ha<sup>−1</sup>) yields were recorded at 138 kg N ha<sup>−1</sup> with 92 kg P<sub>2</sub>O<sub>5</sub> ha<sup>−1</sup>. The highest performance, in terms of economically yield-related parameters, was recorded with the combined application of 138 kg N ha<sup>−1</sup> and 69 kg P<sub>2</sub>O<sub>5</sub> ha<sup>−1</sup>, resulting in biomass and grain yields of 7800 and 3770 kg ha<sup>−1</sup>, respectively. Total nitrogen uptake was high for 138 kg N ha<sup>−1</sup> (82.52 kg N ha<sup>−1</sup>), while phosphorus uptake was high for 69 kg P<sub>2</sub>O<sub>5</sub> ha<sup>−1</sup> (18.42 kg P ha<sup>−1</sup>), with uptake efficiency decreasing at higher rates. The nutrient use efficiency parameter also reached its highest values at the lowest to moderate N and P levels. Partial budget analysis indicates that the applications of 138 kg N ha<sup>−1</sup> with 69 kg P<sub>2</sub>O<sub>5</sub> ha<sup>−1</sup> gave the highest net benefit of 258,954.2 ETB ha<sup>−1</sup>, with a marginal return of 5825.3%. Therefore, the application of 138 kg N ha<sup>−1</sup> and 69 kg P<sub>2</sub>O<sub>5</sub> ha<sup>−1</sup> is recommended for maximizing wheat yield, economic returns, and enhancing nutrient uptake and nutrient efficiency in Wadla and similar agro-ecological areas. Further research should be done on the long-term residual effect of P and nutrient efficiency through regular soil testing and monitoring to enhance nutrient uptake while minimizing environmental footprints.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 4","pages":""},"PeriodicalIF":1.5,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://acsess.onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70248","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145407079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}