Ceskoslovenska epidemiologie, mikrobiologie, imunologie最新文献

The authors evaluated the sensitivity of 35 strains of mycotic agents to eight antifungal preparations, using two methods: the diffuse disc test and the method of assessment of the MIC in a liquid medium. On comparison of results obtained by the two methods they reached the conclusion that the diffuse disc method makes possible only orientation as regards sensitivity and does not fully correlate with the results of MIC. Some strains with MIC values at the sensitivity level seemed resistant according to the disc test. From a total of seven agar media used for the disc test only two were suitable: the medium with casitone and Sabouraud's glucose agar. In particular on the former there were inhibition zones round the discs which were easiest to evaluate and which were constant at different incubation temperatures.

The authors report 10 cases of cysticercosis registered by the National Reference Laboratory for tissue helminthiasis. Six Czech citizens and four foreigners contracted the disease. Four of 6 Czech citizens were contaminated abroad. Six patients suffered from nervous manifestations of cysticercosis, 2 from muscular or subcutaneous cysticercosis and 2 patients had an asymptomatic course of the infection. The prerequisite of successful treatment with new anthelmintics is early diagnostics.

The authors reviewed data in the literature about influencing the virulence factors and other properties of important gram-negative and gram-positive bacteria by sub-MICs of antimicrobial substances. Special attention is devoted to influencing the exo-products as well as adherent properties of Pseudomonas aeruginosa.

Based on previous studies which proved the decisive importance of the degree of bacteraemia of experimentally infected white mice for the degree of infection of ticks Ixodes ricinus the authors confirmed the possibility of transstadial transmission of Francisella tularensis from larvae via nymphs to imagos by the detection of germs in the ticks and by experiments attempting their transmission to white mice. This applied to imagos even one year after the infection of larvae. Transmission was recorded even in imagos which did not transmit the infection in the nymphal stage. F. tularensis was repeatedly detected in the faeces of nymphs and imagos, which along with possible consumption of infected ticks by hosts, indicates further possibilities of transmission of the infection in nature. The results of quantitative examinations of the degree of infection of ticks enable us to conclude that the germs reproduce in the ticks after feeding on the host.

The authors describe a case of salmonella pyarthritis with subsequent osteomyelitis caused by Salmonella enteritidis in a 9-month-old infant. The strain was isolated from punctate drawn from the knee joint.

In the series of 212 coagulase-negative staphylococci strains isolated from blood cultures, in which the slime production was tested by means of two methods--a method using agar with Congo red and a Christensen method--S. epidermidis, S. haemolyticus and S. hominis were identified most frequently (in 69.3%, 18.4% and 6.6%, respectively). The slime production was detected by both methods in 28 strains (13%) of four species, most often in S. epidermidis (in 17%). By comparing the Congo red agar method with the Christensen method the sensitivity and specificity of the former was determined (85% and 99%, respectively). Thus the Congo red agar method for the detection of slime production by staphylococci seems to be a reliable tool.