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Comparative Study of the in vitro Antibacterial Activity of Extracts of Two Penicillium oxalicum Strains on the Growth of Multi-resistant Bacteria 两株草青霉提取物对多重耐药菌生长的体外抑菌活性比较研究
Pub Date : 2022-09-23 DOI: 10.12691/ajmr-10-2-4
Gneho Doh Arioste Delchinor, Pakora Gilles Alex, Y. Dodehe, K. Adele
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引用次数: 0
Emergence of Monkeypox Raises a Serious Challenge to Public Health 猴痘的出现对公共卫生构成严重挑战
Pub Date : 2022-06-29 DOI: 10.12691/ajmr-10-2-2
M. Pal, Kirubel Paulos Gutama
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引用次数: 3
Stenotrophomonas maltophilia an Emerging Opportunistic Bacterial Infection Isolated from Diverse Clinical Samples 嗜麦芽窄养单胞菌是一种从不同临床样本中分离出来的新出现的机会性细菌感染
Pub Date : 2022-06-07 DOI: 10.12691/ajmr-10-2-1
P. Singh, S. Malik, V. Lal
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引用次数: 1
Factorial Review Design Analysis of Different Types of Lymphadenopathy and Causative Factor 不同类型淋巴结病及病因的析因评价设计分析
Pub Date : 2022-05-10 DOI: 10.12691/ajmr-10-1-6
Sengamalam Radhakrishnan, Ravindran Muthukumarasamy
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引用次数: 0
Improvement of Sanitary Quality of the Digue by Use of Lactic Starters 应用乳酸发酵剂提高豆浆卫生质量
Pub Date : 2022-03-21 DOI: 10.12691/ajmr-10-1-2
Tchikoua Roger, Ezo’o Ezo’o J.D, Essia Ngang J.J
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引用次数: 0
Diversity, Abundance and Distribution of Parasites of Medical Importance in Surface Water: A Case Study of Adada River, Enugu State, Nigeria 地表水中具有医学意义的寄生虫的多样性、丰度和分布:以尼日利亚埃努古州阿达达河为例
Pub Date : 2021-11-04 DOI: 10.12691/ajmr-9-4-2
Amadi E.C., E. E.A., Nwangwu C.C, C. S. T., E. J.
Adada River, an all-the-year-round sparkling-clear river, and a very useful surface water was evaluated for parasites of medical importance, their diversity, abundance and distribution as a qualitative microbial risk-assessment (QMRA) factor. Parasites were enumerated by Stoll’s Counting Method and identified by the morphological characteristics of their potentials. 14 genera of parasites were detected in the dry season, and 13 detected in the rainy season. Ten of these were potential human pathogens (Taenia spp, Entamoeba histolytica, Schistosoma mansoni, S. haematobium, Ascaris lumbricoides, Giardia, lamblia, Hookworm, Trichuris trichiura, Strongyloides stercoralis and Enterobius vermicularis). Lowest average parasite per milliliter in the stations was 7.0x10, and highest was 2.2x10/ml. The most frequently encountered parasites were potential human pathogens (Taenia spp, G. lamblia, E. histolytica, hookworm and S. mansoni).Variation in the parasites’ genera between the two seasons was not statistically significant (p < 0.05). In conclusion, Adada River is not potable and suitable for recreation, grazing and agriculture. All-the-year-round sparkling-clear surface water, such as this, may be a health deceit. In QMRA, parasites of medical importance should be a paradigm; likewise, in environmental microbiology, geographical coordinates of sample sites rather than physical landmarks, should be a paradigm, for better follow-ups.
作为定性微生物风险评估(QMRA)因子,对具有医学重要性的寄生虫、其多样性、丰度和分布进行了评估。采用斯托尔计数法对寄生虫进行计数,并根据寄生虫电位的形态特征进行鉴定。旱季共检出寄生虫14属,雨季共检出寄生虫13属。其中10种是潜在的人类病原体(带绦虫、溶组织内阿米巴、曼氏血吸虫、血单胞虫、类蚓蛔虫、贾第鞭毛虫、兰螺旋体、钩虫、毛线虫、粪线虫和蛭肠虫)。各监测站平均寄生虫数最低为7.0 × 10/ml,最高为2.2 × 10/ml。最常见的寄生虫是潜在的人类病原体(带绦虫、兰氏绦虫、溶组织伊夫氏绦虫、钩虫和曼氏伊夫氏绦虫)。季节间寄生虫属数差异无统计学意义(p < 0.05)。综上所述,阿达达河不适合饮用,适合休闲、放牧和农业。像这样一年四季晶莹剔透的地表水,可能是对健康的欺骗。在QMRA中,具有医学重要性的寄生虫应该是一个范例;同样,在环境微生物学中,样品地点的地理坐标而不是物理地标应该是一个范例,以便更好地进行后续工作。
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引用次数: 0
Efficacy of the Vaccines, Their Safety, and Immune Responses against SARS-CoV-2 Infections 疫苗对SARS-CoV-2感染的有效性、安全性和免疫反应
Pub Date : 2021-10-12 DOI: 10.12691/AJMR-9-4-1
M. Pal, K. Gutama
The search for vaccines has been a high priority since the worldwide COVID-19 pandemic was declared. Currently, mRNA-based vaccines, adenovirus-based vaccines, inactivated virus vaccines, and other vaccine platforms are all employed to combat the SARS-CoV-2 virus. BNT162b2 and mRNA-1273 are mRNA-based vaccines. The vaccination appears to be effective against SARS-CoV-2 strains that have emerged since the first study. They have primarily minor side effects, and there are no major safety concerns. Adenovirus-based vaccines are delivered by genetic cargo that is based on non-replicating adenovirus vectors that can increase immune response without the need of adjuvants. This is the case for Ad26.CoV2.S, ChAdOx1 nCoV-19/AZD1222, Gam-COVID-Vac/Sputnik V andAd5-based COVID-19 vaccine. There have been no known incidences of allergy to adenovirus vaccines, unlike mRNA vaccines. Inactivated virus vaccines are a common form of vaccine that has been used for decades. The goal is to render the virus non-infectious while preserving immunogenicity with high-quality antigens in order to trigger an immune response. The researched formaldehyde-inactivated whole-virus SARS-CoV2 vaccine (CoronaVac), as well as WIV04 and HB02, utilize this sort of vaccine formulation. A recombinant protein nanoparticle vaccine named NVX-CoV2373 is made up of trimeric spike glycoproteins with a potent Matrix-M1 adjuvant. Against the variant B.1.1.7 (Alpha), the vaccine appeared to be very effective. Vaccine efficacy against the B.1.351 (Beta) strain, on the other hand, proved to be lower.
自宣布全球COVID-19大流行以来,寻找疫苗一直是重中之重。目前,针对SARS-CoV-2的疫苗有基于mrna的疫苗、基于腺病毒的疫苗、灭活病毒疫苗等多种疫苗平台。BNT162b2和mRNA-1273是基于mrna的疫苗。疫苗接种似乎对自第一次研究以来出现的SARS-CoV-2菌株有效。它们主要有轻微的副作用,没有重大的安全问题。基于腺病毒的疫苗由基于非复制性腺病毒载体的遗传货物递送,无需佐剂即可增强免疫应答。这就是Ad26.CoV2的情况。S、ChAdOx1 nCoV-19/AZD1222、Gam-COVID-Vac/Sputnik V和基于ad5的COVID-19疫苗。与mRNA疫苗不同,腺病毒疫苗没有已知的过敏发生率。灭活病毒疫苗是一种常见的疫苗形式,已经使用了几十年。目标是使病毒不具有传染性,同时保留高质量抗原的免疫原性,以触发免疫反应。研究的甲醛灭活SARS-CoV2全病毒疫苗(CoronaVac)以及WIV04和HB02都使用了这种疫苗配方。一种名为NVX-CoV2373的重组蛋白纳米颗粒疫苗由三聚体刺突糖蛋白和强效Matrix-M1佐剂组成。针对B.1.1.7 (Alpha)变种,该疫苗似乎非常有效。另一方面,疫苗对B.1.351 (β)菌株的效力较低。
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引用次数: 3
Detection of a Gene Involved in Fumonisin Production in F usarium Strains Isolated from Moringa ( Moringa oliefera ) Sold in the Markets of Abidjan (Côte d'Ivoire) 在阿比让(Côte科特迪瓦)市场销售的辣木(Moringa oliefera)中分离的一株富马菌素基因的检测
Pub Date : 2021-10-06 DOI: 10.12691/AJMR-9-3-5
Y. K. Ouattara, Sylvie Mireille Kouamé-Sina, K. J. Coulibaly, Blé Yatanan Casimir, Comoé Koffi Donatien Beni, V. Sabine, A. Touré, A. Dadie
Fumonisins produced by Fusarium constitute a concern group of mycotoxins contaminating food products. In Cote d'Ivoire, data on the sanitary quality of marketed Moringa powder remains unknown. This study was carried out to characterize molds of the genus Fusarium isolated from Moringa powder by detecting the presence of a gene encoding the biosynthesis of fumonisins. A total of 192 Moringa leaf powder samples of approximately 250g each were taken from various sales sites in Abidjan markets including Abobo, Adjame, Koumassi and Yopougon. The isolation and purification of the Fusarium strains were carried out on Sabouraud’s medium supplemented with 10 μg / mL of chloramphenicol. The Polymerase Chain Reaction (PCR) method was used for molecular identification of Fusarium strains and the detection of the presence of the Fum13 gene involved in the fumonisins biosynthesis. The contamination rate of Moringa powder by Fusarium-type molds was 15.43%. The predominantly isolated species was Fusarium solani (11.43%) followed by Fusarium sp (4%). The presence of the Fum13 gene was detected in 62.96% (17/27) of the Fusarium strains isolated. The presence of molds of the genus Fusarium and the detection of a gene coding for the fumonisins biosynthesis, requires the implementation of health safety measures during the manufacturing process of Moringa powder.
镰刀菌产生的伏马菌素构成了污染食品的真菌毒素关注组。在科特迪瓦,市场上销售的辣木粉的卫生质量数据仍然未知。本研究通过检测一个编码伏马菌素生物合成的基因的存在,对从辣木粉中分离的镰刀菌属霉菌进行了表征。从阿比让市场包括阿波波、阿贾姆、库马西和约波贡的各个销售点共采集了192份辣木叶粉样品,每份约250克。在添加10 μg / mL氯霉素的Sabouraud培养基上对镰刀菌进行分离纯化。采用聚合酶链反应(PCR)方法对镰刀菌进行分子鉴定,并检测镰刀菌素生物合成相关基因Fum13的存在。辣木粉被镰刀菌型霉菌污染的比例为15.43%。主要分离种为枯萎菌(Fusarium solani)(11.43%),其次为镰刀菌(Fusarium sp)(4%)。检出62.96%(17/27)镰刀菌携带Fum13基因。由于存在镰刀菌属霉菌,并检测到一种编码福马菌素生物合成的基因,因此需要在辣木粉的生产过程中实施卫生安全措施。
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引用次数: 0
Emerging Role of Biosensors for Detection of Foodborne Pathogens 生物传感器在食源性病原体检测中的新作用
Pub Date : 2021-09-24 DOI: 10.12691/ajmr-9-3-4
M. Pal, M. Bulcha, Milsan Getu Banu, Adugna Girma Lema
Numerous techniques are employed for the detection of various types of pathogens in different foods. Rapid detection of foodborne pathogens is very important by using biosensors. The objectives of this review are to highlight the application of the biosensors in detecting foodborne pathogens, future trends of biosensors technology in foodborne pathogens detection, and limitations of biosensors in the food industry. Biosensors are analytical devices and one of the rapid detection methods that can combat certain limitations of conventional detection techniques, such as portability, time, cost, sensitivity, and others. Biosensors have many applications in different fields. Biosensors in the food industry are used for the detection and identification of foodborne pathogens. The uses of biosensors in food are increasing from day to day due to different factors. In the future, the latest generations, which are autonomous and nanomaterial-based biosensors will emerge. There is also a need to develop a biosensor that is easy to use and has a short detection time.
许多技术被用于检测不同食品中各种类型的病原体。利用生物传感器快速检测食源性致病菌是非常重要的。本文综述了生物传感器在食源性致病菌检测中的应用,生物传感器技术在食源性致病菌检测中的未来发展趋势,以及生物传感器在食品工业中的局限性。生物传感器是一种分析设备,是一种快速检测方法,可以克服传统检测技术的某些局限性,如便携性、时间、成本、灵敏度等。生物传感器在不同领域有着广泛的应用。食品工业中的生物传感器用于检测和鉴定食源性病原体。由于各种因素,生物传感器在食品中的应用日益增加。在未来,最新一代的自主纳米材料生物传感器将会出现。还需要开发一种易于使用且检测时间短的生物传感器。
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引用次数: 0
Urinary Tract Infections and Antimicrobial Sensitivity Patterns of Uropathogens Isolated from Diabetic and Non-diabetic Patients Attending Some Hospitals in Awka Awka部分医院糖尿病与非糖尿病患者尿路感染及抗菌药物敏感性分析
Pub Date : 2021-08-02 DOI: 10.12691/ajmr-9-3-3
Ekwealor Chito Clare, Alaribe Oluchi Juliet, Ogbukagu Chioma Maureen, Alaribe James Romeo, Kyrian-Ogbonna Evelyn Ada
Background: Diabetic patients have been found to be prone to urinary tract infections, and there is a wide gap of information in developing countries regarding the prevalence and antibiotic sensitivity of the pathogens causing this infection. This study was carried out to determine the prevalence, predisposing factors and antibiotic sensitivity of organisms causing urinary tract infections among diabetic patients and non-diabetics in four hospitals in Awka, Anambra State, Nigeria. Method: A total of four hundred and sixty participants (230 diabetic patients and 230 non-diabetics) were enrolled in a cross- sectional study design with 249 males (54.13%) and 211 (45.87 %) females. Clean catch mid-stream urine samples were collected from all participants in sterile containers and analyzed macroscopically and microscopically. Each urine specimen was streaked onto Nutrient agar, MacConkey agar, Cysteine Lactose Electrolyte Deficient agar and Sabouraud’s Dextrose agar, incubated at 37°C for 24h and identified using standard methods. The sensitivity of the isolates to different antibiotics was tested using Kirby-Bauer disc diffusion method. Data obtained were analyzed statistically. Result: The overall prevalence of urinary tract infections among diabetic patients, 63 (27.39%), was significantly higher than that among non-diabetics, 41 (17.83%) (p= 0.014). Gender and previous history of UTI were found to have significant association with urinary tract infection (0.000). Organisms isolated were Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Citrobacter spp, Coagulase negative Staphylococcus, Staphylococcus aureus, Enterococcus faecalis, and Candida albicans. The isolates were sensitive to tested antibiotics with Gentamicin (10µg) and Ceftriaxone (30µg) as most effective against Gram negative bacteria isolates while Ampicillin (10µg) and Chloramphenicol (30µg) were most effective against Gram positive bacteria isolates. Conclusion: The prevalence of UTI is significantly higher in diabetics than in non-diabetics with E. coli being the most common isolate.The importance of antibiotic sensitivity testing before treatment is highly recommended.
研究背景:糖尿病患者易发生尿路感染,在发展中国家,引起这种感染的病原体的患病率和抗生素敏感性信息存在很大差距。本研究旨在确定尼日利亚阿南布拉州Awka的四家医院中糖尿病患者和非糖尿病患者中引起尿路感染的微生物的患病率、易感因素和抗生素敏感性。方法:采用横断面研究设计,共纳入460名参与者(230名糖尿病患者和230名非糖尿病患者),其中男性249名(54.13%),女性211名(45.87%)。在无菌容器中收集所有参与者的中游尿液样本,并进行宏观和微观分析。将每个尿液标本在nutrition琼脂、MacConkey琼脂、半胱氨酸乳糖电解质缺乏琼脂和Sabouraud’s Dextrose琼脂上划线,在37℃下孵育24小时,使用标准方法进行鉴定。采用Kirby-Bauer圆盘扩散法检测菌株对不同抗生素的敏感性。对所得数据进行统计学分析。结果:糖尿病患者尿路感染总患病率为63例(27.39%),显著高于非糖尿病患者41例(17.83%)(p= 0.014)。性别和尿路感染史与尿路感染有显著相关性(0.000)。分离到的微生物有大肠杆菌、肺炎克雷伯菌、奇异变形杆菌、铜绿假单胞菌、柠檬酸杆菌、凝固酶阴性葡萄球菌、金黄色葡萄球菌、粪肠球菌和白色念珠菌。菌株对抗生素敏感,其中庆大霉素(10µg)和头孢曲松(30µg)对革兰氏阴性菌最有效,氨苄西林(10µg)和氯霉素(30µg)对革兰氏阳性菌最有效。结论:尿路感染在糖尿病患者中的患病率明显高于非糖尿病患者,其中大肠杆菌是最常见的分离物。强烈建议在治疗前进行抗生素敏感性测试。
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引用次数: 1
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American Journal of Microbiological Research
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