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CsNWD Encoding VPS62 Emerges as a Candidate Gene Conferring the Glabrous Phenotype in Cucumber 编码 VPS62 的 CsNWD 成为黄瓜无毛表型的候选基因
Pub Date : 2024-09-04 DOI: 10.3390/agronomy14092019
Yuelong Zhou, Mingyuan Xu, Yong Zhou, Zhaoyang Hu, Shiqiang Liu
The commercial value of cucumbers is influenced by the presence of spines or trichomes on their fruit, while the trichomes found on other parts of the plant are essential for protecting the cucumber against a range of biotic and abiotic stresses. In our research, we identified a glabrous nwd mutant from EMS-induced cucumber (Cucumis sativus L.) mutant lines. This mutant displays a consistent glabrous phenotype across all plant organs. Upon analysis, it was revealed that the nwd mutation is governed by a lone recessive nuclear gene. The nwd plants show a significant reduction in both their photosynthetic capacity and chlorophyll a content when compared to the wild-type. Furthermore, there are notable changes in the antioxidant enzyme activities, soluble protein levels, and malondialdehyde content of the nwd mutant. Genotyping and MutMap approaches identified the CsNWD gene, encoding a VPS protein, as a candidate responsible for the glabrous trait. Transcriptome analysis indicated that 15 trichome-related genes exhibit significant expression changes in the nwd mutant, implying that CsNWD may regulate trichome development through interactions with these genes. This study contributes to the understanding of VPS proteins in trichome initiation and the underlying mechanisms of trichome development.
黄瓜果实上的刺或毛状体影响了黄瓜的商业价值,而植株其他部位的毛状体对于保护黄瓜免受一系列生物和非生物胁迫至关重要。在我们的研究中,我们从 EMS 诱导的黄瓜(Cucumis sativus L.)突变体系中发现了一个无毛 nwd 突变体。该突变体的所有植物器官都表现出一致的无毛表型。经分析发现,nwd 突变由一个单独的隐性核基因控制。与野生型相比,nwd 植物的光合能力和叶绿素 a 含量都显著降低。此外,nwd 突变体的抗氧化酶活性、可溶性蛋白水平和丙二醛含量也发生了显著变化。基因分型和 MutMap 方法确定了编码 VPS 蛋白的 CsNWD 基因是导致无毛性状的候选基因。转录组分析表明,在nwd突变体中,15个毛状体相关基因的表达发生了显著变化,这意味着CsNWD可能通过与这些基因的相互作用来调控毛状体的发育。这项研究有助于人们了解 VPS 蛋白在毛状体启动过程中的作用以及毛状体发育的内在机制。
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引用次数: 0
Nutrient Dynamics in Integrated Crop–Livestock Systems: Effects of Stocking Rates and Nitrogen System Fertilization on Litter Decomposition and Release 作物-牲畜综合系统中的养分动态:放养率和氮系统施肥对粪便分解和释放的影响
Pub Date : 2024-09-03 DOI: 10.3390/agronomy14092009
Marcos Antonio de Bortolli, Tangriani Simioni Assmann, Betania Brum de Bortolli, Marcieli Maccari, Angela Bernardon, Jorge Jamhour, Alan J. Franzluebbers, Andre Brugnara Soares, Igor Kieling Severo
Current fertilizer recommendations often neglect nutrient cycling across crop rotations. This study aimed to assess the decay rate and nutrient (N, P, K) release patterns of sorghum, black oat, and corn residues (omitido) in an integrated crop–livestock system. The experiment used factorial treatments based on two sward heights (high and low) and two nitrogen fertilization levels (N-pasture at 200 kg N ha−1 and N-corn at 0 kg N ha−1). Litter bags were collected at various intervals from each crop to measure nutrient release patterns and decomposition rates. The results showed that pasture height and nitrogen fertilization significantly influenced decomposition and nutrient release, affecting the subsequent grain crop phase. Potassium was released rapidly and in high amounts. Nitrogen fertilization during the pasture phase prevented nitrogen and phosphorus immobilization in black oat residue and reduced immobilization in corn residue. These findings highlight the importance of accounting for nutrient cycling and decomposition rates in fertilization strategies to enhance the sustainability of integrated crop–livestock systems.
目前的肥料建议往往忽视了作物轮作中的养分循环。本研究旨在评估作物-牲畜综合系统中高粱、黑燕麦和玉米残留物(omitido)的腐烂率和养分(氮、磷、钾)释放模式。实验采用了基于两种草皮高度(高和低)和两种氮肥水平(氮肥-牧草 200 千克/公顷和氮肥-玉米 0 千克/公顷)的因子处理。每种作物在不同时间间隔收集粪便袋,以测量养分释放模式和分解率。结果表明,牧草高度和氮肥施用对分解和养分释放有显著影响,并影响随后的粮食作物阶段。钾的释放速度快,释放量大。在牧草阶段施氮肥可防止氮和磷固定在黑燕麦残留物中,并减少固定在玉米残留物中。这些发现强调了在施肥策略中考虑养分循环和分解率对提高作物-牲畜综合系统可持续性的重要性。
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引用次数: 0
Effect of Water Tank Size and Supply on Greenhouse-Grown Kidney Beans Irrigated by Rainwater in Cold and Arid Regions of North China 水箱大小和供水量对华北寒冷干旱地区雨水灌溉温室芸豆的影响
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081767
Mengmeng Sun, Jizong Zhang, Zhihui Wang, Jingxin Ran, Yunjie Han, Jianheng Zhang, Huibin Li, Lifeng Zhang
In response to water scarcity in the Bashang area of northwest Hebei Province, a cold and arid region in north China, and to address the diminishing groundwater levels caused by pumping irrigation, this study investigated the impact of rainwater tank size and water supply on kidney beans production in greenhouses under various precipitation scenarios to determine the production potential and development strategies for regional precipitation resources. Under the background of average annual precipitation, kidney bean yield increased with increasing reservoir volume and shorter irrigation cycles. Under a 4-day irrigation cycle, the water demand satisfaction rate of kidney beans reached 100% water demand when the rainwater tank size was 15.7 m3. Against the wide variation in multi-year regional precipitation from 1992 to 2023, the annual effect of rainwater harvest was simulated using precipitation data collected 20 years with an 80% precipitation guarantee rate. The average minimum yield reduction rate obtained was 9.4%, and the corresponding minimum rainwater tank size was 29.5 m3. By superimposing the rainwater harvested in the shed and nonshed areas, the volume of the reservoir without yield reduction could be reduced to 20.0 m3. The sum of discharged and inventory water was much greater than the water scarcity in each water supply situation. Simulating and analyzing the effect of the relationship between rainwater tank size and water supply on rainwater harvesting in regional farmland by year provides important data affecting the construction of regional rainwater storage facilities and water supply efficiency. To achieve a high, stable yield of kidney beans grown in a greenhouse with shed film and shed area rainwater harvesting in north China, 2.6 m3 supplementary groundwater irrigation is still needed during the annual growing season.
河北省西北部是中国北方寒冷干旱地区,为应对该地区缺水问题,并解决抽水灌溉导致地下水位不断下降的问题,本研究调查了不同降水情景下雨水槽大小和供水量对温室芸豆产量的影响,以确定区域降水资源的生产潜力和开发策略。在年平均降水量背景下,芸豆产量随着蓄水池容积的增加和灌溉周期的缩短而增加。在 4 天灌溉周期内,当雨水蓄水池容积为 15.7 立方米时,芸豆的需水满足率达到 100%。针对 1992 年至 2023 年区域多年降水量的巨大差异,利用 20 年来的降水数据模拟了雨水收集的年效应,降水保证率为 80%。得出的平均最小减产率为 9.4%,相应的最小雨水箱容积为 29.5 立方米。通过叠加棚区和非棚区收集的雨水,不减产的蓄水池容积可减少到 20.0 立方米。排放水量和库存水量之和远大于各种供水情况下的缺水量。模拟和分析雨水蓄水池大小与供水量之间的关系对区域农田雨水收集的影响,为区域雨水蓄水设施的建设和供水效率提供了重要的影响数据。华北地区棚膜和棚区集雨温室种植的芸豆要实现高产稳产,每年生长季节仍需补充地下水灌溉 2.6 立方米。
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引用次数: 0
Prediction and Classification of Phenol Contents in Cnidium officinale Makino Using a Stacking Ensemble Model in Climate Change Scenarios 利用堆叠集合模型预测气候变化情景下 Makino 蛇尾草中的酚含量并对其进行分类
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081766
Hyunjo Lee, Hyun Jung Koo, Kyeong Cheol Lee, Yoojin Song, Won-Kyun Joo, Cheol-Joo Chae
Recent studies have focused on using big-data-based machine learning to address the effects of climate change scenarios on the production and quality of medicinal plants. Challenges relating to data collection can hinder the analysis of key feature variables that affect the quality of medicinal plants. In the study presented herein, we analyzed feature variables that affect the phenolic content of Korean Cnidium officinale Makino (C. officinale Makino) under different climate change scenarios. We applied different climate change scenarios based on environmental information obtained from Yeongju city, Gyeongsangbuk-do, Republic of Korea, and cultivated C. officinale Makino to collect data. The collected data included 3237, 75, and 45 records, and data augmentation was performed to address this data imbalance. We designed a function based on the DPPH value to set the phenolic content grade in C. officinale Makino and proposed a stacking ensemble model for predicting the total phenol contents and classifying the phenolic content grades. The regression model in the performance evaluation presented an improvement of 6.23–7.72% in terms of the MAPE; in comparison, the classification model demonstrated a 2.48–3.34% better performance in terms of accuracy. The classification accuracy was >0.825 when classifying phenol content grades using the predicted total phenol content values from the regression model, and the area under the curve values of the model indicated high model fitness (0.987–0.981). We plan to identify the key feature variables for the optimal cultivation of C. officinale Makino and explore the relationships among these feature variables.
最近的研究侧重于利用基于大数据的机器学习来解决气候变化情景对药用植物生产和质量的影响。与数据收集有关的挑战可能会阻碍对影响药用植物质量的关键特征变量的分析。在本文介绍的研究中,我们分析了在不同气候变化情景下影响韩国蛇床子(C. officinale Makino)酚含量的特征变量。我们根据从大韩民国庆尚北道荣州市获得的环境信息,应用了不同的气候变化情景,并对栽培的 C. officinale Makino 进行了数据收集。收集到的数据包括 3237 条记录、75 条记录和 45 条记录,为解决数据不平衡问题,我们进行了数据扩充。我们设计了一个基于 DPPH 值的函数来设定牧野甘蓝的酚含量等级,并提出了一个用于预测总酚含量和划分酚含量等级的堆叠集合模型。在性能评估中,回归模型的 MAPE 提高了 6.23-7.72%;相比之下,分类模型的准确度提高了 2.48-3.34%。利用回归模型预测的总酚含量值进行酚含量等级分类时,分类准确度大于 0.825,模型的曲线下面积值表明模型适配性很高(0.987-0.981)。我们计划确定牧野甘蓝最佳栽培的关键特征变量,并探索这些特征变量之间的关系。
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引用次数: 0
Tomato Recognition Method Based on the YOLOv8-Tomato Model in Complex Greenhouse Environments 复杂温室环境中基于 YOLOv8 番茄模型的番茄识别方法
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081764
Shuhe Zheng, Xuexin Jia, Minglei He, Zebin Zheng, Tianliang Lin, Wuxiong Weng
Tomatoes are a critical economic crop. The realization of tomato harvesting automation is of great significance in solving the labor shortage and improving the efficiency of the current harvesting operation. Accurate recognition of fruits is the key to realizing automated harvesting. Harvesting fruit at optimum ripeness ensures the highest nutrient content, flavor and market value levels, thus maximizing economic benefits. Owing to foliage and non-target fruits obstructing target fruits, as well as the alteration in color due to light, there is currently a low recognition rate and missed detection. We take the greenhouse tomato as the object of research. This paper proposes a tomato recognition model based on the improved YOLOv8 architecture to adapt to detecting tomato fruits in complex situations. First, to improve the model’s sensitivity to local features, we introduced an LSKA (Large Separable Kernel Attention) attention mechanism to aggregate feature information from different locations for better feature extraction. Secondly, to provide a higher quality upsampling effect, the ultra-lightweight and efficient dynamic upsampler Dysample (an ultra-lightweight and efficient dynamic upsampler) replaced the traditional nearest neighbor interpolation methods, which improves the overall performance of YOLOv8. Subsequently, the Inner-IoU function replaced the original CIoU loss function to hasten bounding box regression and raise model detection performance. Finally, the model test comparison was conducted on the self-built dataset, and the test results show that the mAP0.5 of the YOLOv8-Tomato model reached 99.4% and the recall rate reached 99.0%, which exceeds the original YOLOv8 model detection effect. Compared with faster R-CNN, SSD, YOLOv3-tiny, YOLOv5, and YOLOv8 models, the average accuracy is 7.5%, 11.6%, 8.6%, 3.3%, and 0.6% higher, respectively. This study demonstrates the model’s capacity to efficiently and accurately recognize tomatoes in unstructured growing environments, providing a technical reference for automated tomato harvesting.
番茄是一种重要的经济作物。实现番茄采收自动化对于解决劳动力短缺问题、提高现有采收作业效率具有重要意义。准确识别果实是实现自动化采收的关键。在最佳成熟期收获果实可确保最高的营养含量、风味和市场价值水平,从而实现经济效益最大化。由于叶片和非目标水果会遮挡目标水果,再加上光照会改变水果的颜色,目前识别率较低,存在漏检现象。我们以温室番茄为研究对象。本文提出了一种基于改进的 YOLOv8 架构的番茄识别模型,以适应复杂情况下番茄果实的检测。首先,为了提高模型对局部特征的敏感度,我们引入了 LSKA(Large Separable Kernel Attention)注意力机制,以聚合不同位置的特征信息,从而实现更好的特征提取。其次,为了提供更高质量的上采样效果,超轻量级高效动态上采样器 Dysample(一种超轻量级高效动态上采样器)取代了传统的近邻插值方法,从而提高了 YOLOv8 的整体性能。随后,Inner-IoU 函数取代了原来的 CIoU 损失函数,加速了边界框回归,提高了模型检测性能。最后,在自建数据集上进行了模型测试比较,测试结果表明,YOLOv8-Tomato 模型的 mAP0.5 达到 99.4%,召回率达到 99.0%,超过了原有 YOLOv8 模型的检测效果。与速度更快的 R-CNN、SSD、YOLOv3-tiny、YOLOv5 和 YOLOv8 模型相比,平均准确率分别提高了 7.5%、11.6%、8.6%、3.3% 和 0.6%。这项研究表明,该模型能够在非结构化种植环境中高效、准确地识别番茄,为番茄自动收获提供了技术参考。
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引用次数: 0
Laboratory Evaluation of the Combine Usage Possibilities of Entomopathogenic Nematodes with Insecticides against Mediterranean Corn Borer Sesamia nonagrioides (Lefebvre) 昆虫病原线虫与杀虫剂联合防治地中海玉米螟(Sesamia nonagrioides (Lefebvre))的可能性实验室评估
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081763
Esengül Erdem
The Mediterranean Corn Borer (MCB), Sesamia nonagrioides, poses a significant threat to maize crops, necessitating effective pest management strategies. This study investigates the compatibility of two entomopathogenic nematode (EPN) isolates, Steinernema feltiae KV6 and Heterorhabditis bacteriophora EO7, with four registered insecticides for MCB control: deltamethrin, flubendiamide, spinetoram, and betacyfluthrin. The impact of these insecticides on EPN mortality, infectivity, and reproduction was assessed. Results indicate that deltamethrin exhibits the lowest toxicity to EPNs, with mortality rates of 1.3% for S. feltiae and 0.63% for H. bacteriophora at field dose (FD) after 24 h and 4.63% and 1.96%, respectively, after 48 h. In contrast, betacyfluthrin showed higher toxicity, with mortality rates of 38.04% and 14.17% for S. feltiae at 2FD and FD after 48 h. The infectivity assays demonstrated that deltamethrin-treated EPNs caused up to 100% mortality in MCB larvae, while the reproduction capacity varied significantly between the EPN species and insecticides. H. bacteriophora exhibited higher progeny production, especially in the presence of deltamethrin (87,900 IJs/larva). The findings suggest that integrating EPNs with selective insecticides like deltamethrin can enhance pest control efficacy and support sustainable agricultural practices. This study provides valuable insights for developing integrated pest management (IPM) strategies aimed at mitigating MCB infestations in maize while minimizing environmental impacts.
地中海玉米螟(MCB)(Sesamia nonagrioides)对玉米作物构成严重威胁,因此必须采取有效的虫害防治策略。本研究调查了两种昆虫病原线虫(EPN)分离株(Steinernema feltiae KV6 和 Heterorhabditis bacteriophora EO7)与四种用于控制 MCB 的登记杀虫剂(溴氰菊酯、氟苯虫酰胺、辛硫磷和倍硫磷菊酯)的相容性。评估了这些杀虫剂对 EPN 死亡率、传染性和繁殖的影响。结果表明,溴氰菊酯对 EPN 的毒性最低,在田间剂量(FD)下,24 小时后毛毡蝇的死亡率为 1.3%,细菌弧菌的死亡率为 0.63%,48 小时后死亡率分别为 4.63%和 1.96%。感染性试验表明,溴氰菊酯处理的 EPN 对 MCB 幼虫的致死率高达 100%,而 EPN 种类和杀虫剂之间的繁殖能力存在显著差异。细菌嗜血杆菌的后代产量较高,尤其是在使用溴氰菊酯的情况下(87 900 IJs/幼虫)。研究结果表明,将 EPNs 与溴氰菊酯等选择性杀虫剂结合使用,可以提高害虫控制效果,支持可持续农业实践。这项研究为制定虫害综合防治(IPM)策略提供了宝贵的见解,旨在减轻玉米中 MCB 的虫害,同时最大限度地减少对环境的影响。
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引用次数: 0
Evaluating Impacts of Biochar and Inorganic Fertilizer Applications on Soil Quality and Maize Yield Using Principal Component Analysis 利用主成分分析法评估施用生物炭和无机肥料对土壤质量和玉米产量的影响
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081761
Oluwaseun Temitope Faloye, Ayodele Ebenezer Ajayi, Viroon Kamchoom, Olayiwola Akin Akintola, Philip Gbenro Oguntunde
A 2-year field experiment was conducted to test the effects of individual and co-application of biochar and inorganic fertilizer on soil quality using the principal component analysis (PCA) technique. The dry season field experiments were performed with biochar applied at 0 and 20 t ha−1, and fertilizer at 300 and 0 kg ha−1 (control). The factorial combinations of the above-mentioned treatments were subjected to irrigation at 60, 80, and 100% of irrigation amounts (IAs). Soil hydro-physical and chemical properties and grain yield were determined at harvest. Results from the PCA indicated that the soil total nitrogen (N) and moisture content (MC) were the soil properties mostly affecting the grain yield. The amendments’ effects on the soil physico-chemical properties and maize yield were in the order control < biochar < fertilizer < biochar + fertilizer. The derived comprehensive soil quality index (CSQI) from the PCA showed that the soil quality increased by 76, 100, and 200% in treatments individually applied with biochar, inorganic fertilizer, and the co-applications. This study therefore showed that the PCA revealed the actual dynamics in soil properties, in terms of the SQI upon the soil amendment addition, as well as their relationship with maize yield under different weather conditions.
通过为期两年的田间试验,采用主成分分析(PCA)技术测试了单独施用和联合施用生物炭和无机肥对土壤质量的影响。旱季田间试验的生物炭施用量为 0 吨/公顷和 20 吨/公顷,化肥施用量为 300 千克/公顷和 0 千克/公顷(对照)。上述处理的因子组合分别以 60、80 和 100%的灌溉量(IAs)进行灌溉。收获时测定土壤水文物理和化学特性以及谷物产量。PCA 结果表明,土壤全氮(N)和含水量(MC)是对谷物产量影响最大的土壤特性。改良剂对土壤理化性质和玉米产量的影响顺序为对照 < 生物炭 < 肥料 < 生物炭 + 肥料。根据 PCA 得出的土壤综合质量指数(CSQI)显示,在单独施用生物炭、无机肥和联合施用的处理中,土壤质量分别提高了 76%、100% 和 200%。因此,这项研究表明,PCA 揭示了土壤改良剂添加后土壤质量指数(SQI)的实际动态变化,以及它们在不同气候条件下与玉米产量的关系。
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引用次数: 0
Tillage Practices Effect on Root Distribution and Variation of Soil CO2 Emission under Different Cropping Strategies 不同耕作策略下耕作方法对根系分布和土壤二氧化碳排放量变化的影响
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081768
Agnė Buivydienė, Irena Deveikytė, Agnė Veršulienė, Virginijus Feiza
Conservation soil management strategies, particularly no-tillage and cropping strategies, have become an effective and widely adopted practice that has a direct influence on root parameters and mitigation of greenhouse gasses. However, the effect of different tillage and cropping strategies on root growth in field conditions is rarely studied. The study aimed to quantify and characterise the relationship between root network development and CO2 emission and how these parameters are affected by different cropping and tillage strategies. Five different crop rotations were tested, with or without the inclusion of catch crops (CC), by growing them in the soil where different tillage practices were applied. Selected cropping strategies differed among themselves in terms of the frequency of CC grown per rotation. The data revealed that in NT treatments, the CO2 emission (both autotrophic and heterotrophic simultaneously) was 25% higher than in CT. The cropping strategies were identified as an important factor influencing CO2 emissions. An increase in CO2 emission varied between 30 and 35% depending on the share of legume and CC inclusion. The frequency of CC grown per rotation has had an effect on the rate of CO2 emission. The cropping strategy when CC was grown every year showed the lowest amount of CO2 (by 26%), while in other cropping strategies, when CC was grown once or twice per rotation, significantly higher CO2 emissions were observed. Root growth and their development were significantly affected (p < 0.05) by soil depth and cropping strategies concerning root length and root volume changes. The inclusion of CC into the rotations led towards a decrease in root volume (by 21%). Root length (R2 = 0.45; p < 0.05) and root volume (R2 = 0.82; p < 0.05) had a significant impact on soil CO2 emissions. The results collected from 2021 to 2023 experiments indicated that cropping strategies and CC management areas are important tools not only for the improvement of root parameters but also for understanding how they affect CO2 emissions. The main message for stakeholders is that the cropping strategies diversification with the inclusion of CC every year in a winter oil-seed rape, spring wheat and pea crop rotation (R/W/P + CC) had demonstrated the possibilities to reduce CO2 emission and improve the root network parameters as compared to the monoculture strategy.
土壤保持管理策略,尤其是免耕和种植策略,已成为一种有效且被广泛采用的做法,对根系参数和温室气体减排有直接影响。然而,在田间条件下,不同耕作和种植策略对根系生长的影响却鲜有研究。本研究旨在量化和描述根系网络发展与二氧化碳排放之间的关系,以及这些参数如何受到不同种植和耕作策略的影响。通过在采用不同耕作方式的土壤中种植五种不同的轮作作物,测试了是否加入接茬作物(CC)。选定的耕作策略在每次轮作中种植 CC 的频率方面存在差异。数据显示,NT 处理的二氧化碳排放量(同时包括自养和异养)比 CT 处理高 25%。种植策略被认为是影响二氧化碳排放量的一个重要因素。根据豆科植物和 CC 的种植比例,CO2 排放量的增幅在 30% 到 35% 之间。每轮种植 CC 的频率对二氧化碳排放量有影响。在每年种植 CC 的种植策略中,二氧化碳排放量最低(26%),而在其他种植策略中,当每轮种植 CC 一到两次时,二氧化碳排放量明显较高。根的生长和发育受到土壤深度和种植策略的显著影响(p < 0.05),涉及根的长度和体积变化。在轮作中加入 CC 会导致根系体积减少(21%)。根长(R2 = 0.45;p < 0.05)和根量(R2 = 0.82;p < 0.05)对土壤二氧化碳排放量有显著影响。从 2021 年到 2023 年收集的实验结果表明,种植策略和 CC 管理区不仅是改善根系参数的重要工具,也是了解它们如何影响二氧化碳排放的重要工具。对利益相关者的主要启示是,与单一种植策略相比,在冬油菜、春小麦和豌豆轮作(R/W/P + CC)中每年加入 CC 的多样化种植策略证明了减少二氧化碳排放和改善根网参数的可能性。
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引用次数: 0
IIIVmrMLM Provides New Insights into the Genetic Basis of the Agronomic Trait Variation in Chickpea IIIVmrMLM 为了解鹰嘴豆农艺性状变异的遗传基础提供了新见解
Pub Date : 2024-08-12 DOI: 10.3390/agronomy14081762
Maria Duk, Alexander Kanapin, Ekaterina Orlova, Maria Samsonova
Chickpea is a staple crop for many nations worldwide. Modeling genotype-by-environment interactions and assessing the genotype’s ability to contribute adaptive alleles are crucial for chickpea breeding. In this study, we evaluated 12 agronomically important traits of 159 accessions from the N.I. Vavilov All Russian Institute for Plant Genetic Resources collection. These included 145 landraces and 13 cultivars grown in different climatic conditions in Kuban (45°18′ N and 40°52′ E) in both 2016 and 2022, as well as in Astrakhan (46°06′ N and 48°04′ E) in 2022. Using the IIIVmrMLM model in multi-environmental mode, we identified 161 quantitative trait nucleotides (QTNs) with stable genetic effects across different environments. Furthermore, we have observed 254 QTN-by-environment interactions with distinct environment-specific effects. Notably, five of these interactions manifested large effects, with R2 values exceeding 10%, while the highest R2 value for stable QTNs was 4.7%. Within the protein-coding genes and their 1 Kb flanking regions, we have discerned 22 QTNs and 45 QTN-by-environment interactions, most likely tagging the candidate causal genes. The landraces obtained from the N.I Vavilov All Russian Institute for Plant Genetic Resources collection exhibit numerous favorable alleles at quantitative trait nucleotide loci, showing stable effects in the Kuban and Astrakhan regions. Additionally, they possessed a significantly higher number of Kuban-specific favorable alleles of the QTN-by-environment interaction loci compared to the Astrakhan-specific ones. The environment-specific alleles found at the QTN-by-environment interaction loci have the potential to enhance chickpea adaptation to specific climatic conditions.
鹰嘴豆是世界上许多国家的主食作物。模拟基因型与环境的相互作用以及评估基因型贡献适应性等位基因的能力对于鹰嘴豆育种至关重要。在这项研究中,我们评估了 N.I. Vavilov 全俄植物遗传资源研究所收集的 159 个加入品系的 12 个重要农艺性状。其中包括 2016 年和 2022 年在库班(北纬 45°18′,东经 40°52′)以及 2022 年在阿斯特拉罕(北纬 46°06′,东经 48°04′)不同气候条件下种植的 145 个陆地品种和 13 个栽培品种。利用多环境模式下的 IIIVmrMLM 模型,我们确定了 161 个在不同环境中具有稳定遗传效应的数量性状核苷酸(QTN)。此外,我们还观察到 254 个 QTN 与环境的相互作用,这些相互作用具有明显的环境特异性效应。值得注意的是,这些相互作用中有五个表现出较大的效应,R2 值超过 10%,而稳定 QTN 的最高 R2 值为 4.7%。在蛋白质编码基因及其 1 Kb 侧翼区域内,我们发现了 22 个 QTN 和 45 个 QTN 与环境的相互作用,很可能标记了候选的因果基因。从全俄罗斯植物遗传资源研究所(N.I Vavilov All Russian Institute for Plant Genetic Resources)收集的陆稻品种在数量性状核苷酸位点上表现出许多有利的等位基因,在库班和阿斯特拉罕地区显示出稳定的效应。此外,与阿斯特拉罕特异性等位基因相比,它们在 QTN 与环境相互作用位点上拥有更多的库班特异性有利等位基因。在 QTN-环境互作位点上发现的环境特异性等位基因有可能增强鹰嘴豆对特定气候条件的适应性。
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引用次数: 0
Optimizing Ridge–Furrow Rainwater-Harvesting Strategies for Potato Cultivation in the Drylands of Northwestern China: A Regional Approach 优化中国西北旱地马铃薯种植的垄沟雨水收集策略:区域方法
Pub Date : 2024-08-11 DOI: 10.3390/agronomy14081759
Lina Zhang, Siqi Ren, Feifei Pan, Jianshuo Zhou, Jingyan Jiang, Xuebiao Pan, Jing Wang, Baoru Sun, Qi Hu
The arid and semi-arid region of Northwest China plays a significant role in potato production, yet yields are often hampered by drought due to limited precipitation and irrigation water. The ridge–furrow rainwater-harvesting technology is an efficient and widely used technique to relieve drought impact and improve crop yield by changing the micro-topography to harvest rainwater to meet the water demand of crops. An analysis of precipitation, water demand, and runoff data spanning 30 years guided the selection of suitable rainwater-harvesting methods tailored to meteorological conditions. The results showed that potato water demand exceeded precipitation in the region. The mulching approach performed best in the western arid region with the most significant increase in yield and water use efficiency (WUE) and was suitable for the western semi-arid region and the agro-pastoral ecotone. In the potato dryland farming areas, the water deficit increased from southeast to northwest. Specifically, northern Gansu, northern Ningxia, and midwestern Inner Mongolia experienced a water deficit of over 200 mm, and rainwater harvesting combined with irrigation was recommended. Conversely, regarding deficits below 200 mm in southern Gansu, Ningxia, and central Inner Mongolia, a 1:1 or 2:1 pattern of ridges could be applied, and mulching was needed only in the necessary areas. For the southern Qinghai, Shaanxi, and eastern Inner Mongolia regions, ridge–furrow rainwater harvesting could be replaced by flat potato cropping. In summary, rainwater harvesting addresses water deficits, aiding climate adaptation in Northwest China’s arid and semi-arid regions. The implementation of mulching and ridge–furrow technology must be location-specific.
中国西北干旱和半干旱地区在马铃薯生产中发挥着重要作用,但由于降水和灌溉用水有限,产量经常受到干旱的影响。垄作雨水收集技术是一种高效且广泛应用的技术,通过改变微地形来收集雨水,满足作物的需水量,从而缓解干旱影响,提高作物产量。通过对 30 年的降水量、需水量和径流量数据进行分析,选择了适合气象条件的雨水收集方法。结果表明,该地区马铃薯的需水量超过了降水量。地膜覆盖方法在西部干旱地区表现最佳,产量和水分利用效率(WUE)的提高最为显著,同时也适用于西部半干旱地区和农牧生态区。在马铃薯旱作区,水分亏缺从东南向西北增加。具体而言,甘肃北部、宁夏北部和内蒙古中西部的缺水量超过 200 毫米,建议采用集雨与灌溉相结合的方法。相反,甘肃南部、宁夏和内蒙古中部的缺水量低于 200 毫米,可采用 1:1 或 2:1 的田埂模式,只需在必要的地区进行地膜覆盖。而在青海南部、陕西和内蒙古东部地区,则可以用平地马铃薯种植代替脊垄雨水收集。总之,雨水收集可解决缺水问题,有助于中国西北干旱和半干旱地区适应气候。地膜覆盖和脊犁技术的实施必须因地制宜。
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Agronomy
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