Endophytic bacteria from pea (Pisum sativum L.) plants play important roles in regulating plant growth, health, and nutrition. To enhance the understanding of endophytic bacteria in peas, twenty pea cultivars, two chickpeas, and two broad bean cultivars were planted into artificial soils for 4 weeks. Leaves and roots were collected from plants and sterilized. Endophytic bacterial DNAs were isolated from sterilized materials (leaves, roots, and seeds) and used as templates to detect the bacterial diversity by amplifying the 16S V3–V4 region. The Remel Tryptose Soya Agar (TSA) medium, the aluminum sec-butoxide (ASb) medium, and the yeast extract mannitol agar (YMA) medium were used to isolate bacteria from sterilized leaves and roots, respectively. The plant growth-promoting (PGP) properties of these isolated bacteria, such as the solubilization of phosphorus and potassium and the production of Indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, nitrogenase, pectinase, and cellulose, were studied in vitro. Bacterial isolates were processed for 16S rDNA gene sequencing and performed molecular identification by reconstruction of the phylogenetic tree using the neighborhood association approach in the software MEGA X. Results indicated that the majority of the bacterial communities were shared among leaves, roots, and seeds of pea plants. In both the leaves and roots of pea plants, the prominent phyla identified were Pseudomonadota, Bacteroidota, and Bacillota, with dominant genera such as Rhizobium, Bacteroides, Blautia, and Prevotella prevailing at the genus level. The samples from leaves and roots had unique dominant bacterial genera. In total, 48 endophytic bacteria strains were isolated from leaves and roots, of which 16 strains were from roots and 32 strains were from leaves. The majority of the isolates from leaves (78.13%) and roots (75%) had the ability to produce indole-3-acetic acid (IAA). Moreover, isolates from roots also had greater ability to produce 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase (81.25%) than those from leaves (62.5%). This study demonstrated the unique distribution of endophytes in leaves and roots of pea, which can have great potential in pea production.
{"title":"Community Diversity of Endophytic Bacteria in the Leaves and Roots of Pea Seedlings","authors":"Junjie Hao, Quanlan Liu, Fengjing Song, Xiao Cui, Lu Liu, Liping Fu, Shouan Zhang, Xingbo Wu, Xiaoyan Zhang","doi":"10.3390/agronomy14092030","DOIUrl":"https://doi.org/10.3390/agronomy14092030","url":null,"abstract":"Endophytic bacteria from pea (Pisum sativum L.) plants play important roles in regulating plant growth, health, and nutrition. To enhance the understanding of endophytic bacteria in peas, twenty pea cultivars, two chickpeas, and two broad bean cultivars were planted into artificial soils for 4 weeks. Leaves and roots were collected from plants and sterilized. Endophytic bacterial DNAs were isolated from sterilized materials (leaves, roots, and seeds) and used as templates to detect the bacterial diversity by amplifying the 16S V3–V4 region. The Remel Tryptose Soya Agar (TSA) medium, the aluminum sec-butoxide (ASb) medium, and the yeast extract mannitol agar (YMA) medium were used to isolate bacteria from sterilized leaves and roots, respectively. The plant growth-promoting (PGP) properties of these isolated bacteria, such as the solubilization of phosphorus and potassium and the production of Indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, nitrogenase, pectinase, and cellulose, were studied in vitro. Bacterial isolates were processed for 16S rDNA gene sequencing and performed molecular identification by reconstruction of the phylogenetic tree using the neighborhood association approach in the software MEGA X. Results indicated that the majority of the bacterial communities were shared among leaves, roots, and seeds of pea plants. In both the leaves and roots of pea plants, the prominent phyla identified were Pseudomonadota, Bacteroidota, and Bacillota, with dominant genera such as Rhizobium, Bacteroides, Blautia, and Prevotella prevailing at the genus level. The samples from leaves and roots had unique dominant bacterial genera. In total, 48 endophytic bacteria strains were isolated from leaves and roots, of which 16 strains were from roots and 32 strains were from leaves. The majority of the isolates from leaves (78.13%) and roots (75%) had the ability to produce indole-3-acetic acid (IAA). Moreover, isolates from roots also had greater ability to produce 1-amino-cyclopropane-1-carboxylic acid (ACC) deaminase (81.25%) than those from leaves (62.5%). This study demonstrated the unique distribution of endophytes in leaves and roots of pea, which can have great potential in pea production.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"103 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In order to increase nutrient input and alleviate the poor growth of blueberry (Vaccinium corymbosum L.) in neutral soil with strong nitrification, the application of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) as an enhanced efficiency fertilizer is a strategy to reduce nitrogen (N) loss and improve N supply. However, few studies have systematically investigated the effect of DMPP application on blueberry and its soil condition in detail so far. In this study, a pot experiment was conducted to elucidate the effect of DMPP at four gradient levels including 0.5% (w/w applied-N) DMPP (DL), 1% DMPP (DM), 2% DMPP (DH), and no DMPP (CK) on the dynamics of soil mineral N (NH4+-N and NO3−-N), soil chemical properties, as well as the agronomic characteristics and physiological indexes of blueberry plants in the neutral soil–blueberry system. The addition of DMPP significantly increased the retention of soil ammonium nitrogen and the content of total mineral nitrogen. qPCR analysis showed that DMPP inhibited the ammoxidation process mainly by reducing the abundance of the ammonia-oxidizing bacteria (AOB) amoA gene rather than the ammonia-oxidizing archaea (AOA) amoA gene. No significant inhibitory effect of DMPP was observed for the nitrite dehydrogenase gene nxrA and nitrite reductase gene nirS. Soil NH4+-N and available phosphorus content were both enhanced with the DMPP application rates both in bulk and rhizosphere soil. Applying 1% DMPP to the neutral soil for blueberry was sufficient to safely inhibit soil nitrification, not only increasing ammonium nitrogen content by 10.42% and 26.79%, but also enhancing available phosphorus content by 9.19% and 22.41% compared with CK in bulk and rhizosphere soil, respectively. Moreover, 1% DMPP addition increased the nitrogen and phosphorus concentration of blueberry leaves by 12.17% and 26.42%, respectively, compared with CK. The total branch length and the dry weight of blueberry plant were also increased by 16.8% and 33.1%, respectively. These results provide valuable agronomic information for the application of DMPP in blueberry cultivation. Fertilization applied with 1% DMPP has great economic potential to improve both nitrogen and phosphorus absorption of blueberry so as to promote the vegetative growth of blueberry.
{"title":"Effect of the Nitrification Inhibitor DMPP on Blueberry Planted in Neutral Soil","authors":"Yiru Yang, Qilong Zeng, Hong Yu, Jiguang Wei, Jiafeng Jiang, Liangliang Tian","doi":"10.3390/agronomy14092029","DOIUrl":"https://doi.org/10.3390/agronomy14092029","url":null,"abstract":"In order to increase nutrient input and alleviate the poor growth of blueberry (Vaccinium corymbosum L.) in neutral soil with strong nitrification, the application of nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) as an enhanced efficiency fertilizer is a strategy to reduce nitrogen (N) loss and improve N supply. However, few studies have systematically investigated the effect of DMPP application on blueberry and its soil condition in detail so far. In this study, a pot experiment was conducted to elucidate the effect of DMPP at four gradient levels including 0.5% (w/w applied-N) DMPP (DL), 1% DMPP (DM), 2% DMPP (DH), and no DMPP (CK) on the dynamics of soil mineral N (NH4+-N and NO3−-N), soil chemical properties, as well as the agronomic characteristics and physiological indexes of blueberry plants in the neutral soil–blueberry system. The addition of DMPP significantly increased the retention of soil ammonium nitrogen and the content of total mineral nitrogen. qPCR analysis showed that DMPP inhibited the ammoxidation process mainly by reducing the abundance of the ammonia-oxidizing bacteria (AOB) amoA gene rather than the ammonia-oxidizing archaea (AOA) amoA gene. No significant inhibitory effect of DMPP was observed for the nitrite dehydrogenase gene nxrA and nitrite reductase gene nirS. Soil NH4+-N and available phosphorus content were both enhanced with the DMPP application rates both in bulk and rhizosphere soil. Applying 1% DMPP to the neutral soil for blueberry was sufficient to safely inhibit soil nitrification, not only increasing ammonium nitrogen content by 10.42% and 26.79%, but also enhancing available phosphorus content by 9.19% and 22.41% compared with CK in bulk and rhizosphere soil, respectively. Moreover, 1% DMPP addition increased the nitrogen and phosphorus concentration of blueberry leaves by 12.17% and 26.42%, respectively, compared with CK. The total branch length and the dry weight of blueberry plant were also increased by 16.8% and 33.1%, respectively. These results provide valuable agronomic information for the application of DMPP in blueberry cultivation. Fertilization applied with 1% DMPP has great economic potential to improve both nitrogen and phosphorus absorption of blueberry so as to promote the vegetative growth of blueberry.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092026
Antonio M. Cabrera-Ariza, Miguel Aguilera-Peralta, Rómulo Santelices-Moya
To achieve sustainable and profitable production of sorghum for energy purposes, it is crucial to ensure the efficient use of the nutrients necessary for its growth and development. This research investigates the influence of diverse management practices on biomass production, nutrient use efficiency, and nitrogen balance in two sorghum hybrids cultivated for bioenergy applications. A comprehensive field study was conducted over two growing seasons, evaluating the effects of fertilization methods and crop rotation strategies. Results indicate that high nitrogen (HN) fertilization increased dry biomass production (up to 20.7 Mg ha−1) and nutrient removal (up to 343.5 kg K ha−1) in both sorghum hybrids. The H128 hybrid showed higher nutrient use efficiency, especially for phosphorus, while the nitrogen balance was positive under HN but varied under low nitrogen (LN), with the H133 hybrid experiencing a net nitrogen loss at LN. These findings contribute valuable insights into sustainable sorghum cultivation for bioenergy production, highlighting the importance of tailored management practices in achieving optimal crop performance.
{"title":"Comparative Efficiency of Nitrogen Fertilization Levels in Two Sorghum Hybrids for Bioenergy Production","authors":"Antonio M. Cabrera-Ariza, Miguel Aguilera-Peralta, Rómulo Santelices-Moya","doi":"10.3390/agronomy14092026","DOIUrl":"https://doi.org/10.3390/agronomy14092026","url":null,"abstract":"To achieve sustainable and profitable production of sorghum for energy purposes, it is crucial to ensure the efficient use of the nutrients necessary for its growth and development. This research investigates the influence of diverse management practices on biomass production, nutrient use efficiency, and nitrogen balance in two sorghum hybrids cultivated for bioenergy applications. A comprehensive field study was conducted over two growing seasons, evaluating the effects of fertilization methods and crop rotation strategies. Results indicate that high nitrogen (HN) fertilization increased dry biomass production (up to 20.7 Mg ha−1) and nutrient removal (up to 343.5 kg K ha−1) in both sorghum hybrids. The H128 hybrid showed higher nutrient use efficiency, especially for phosphorus, while the nitrogen balance was positive under HN but varied under low nitrogen (LN), with the H133 hybrid experiencing a net nitrogen loss at LN. These findings contribute valuable insights into sustainable sorghum cultivation for bioenergy production, highlighting the importance of tailored management practices in achieving optimal crop performance.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092022
Mohamed Nejib El Melki, Imen Soussi, Jameel Mohammed Al-Khayri, Othman M. Al-Dossary, Bader Alsubaie, Slaheddine Khlifi
This study evaluates the projected impact of climate change on wheat production in Northwest Tunisia, specifically at Medjez El Beb (36.67 m, 9.74°) and Slougia (36.66 m, 9.6°), for the period 2041–2070. Using the CNRM-CM5.1 and GFDL-ESM2M climate models under RCP4.5 and RCP8.5 scenarios, coupled with the AquaCrop and SIMPLE crop growth models, we compared model outputs with observed data from 2016 to 2020 to assess model performance. The objective was to determine how different climate models and scenarios affect wheat yields, biomass, and growth duration. Under RCP4.5, projected average yields are 7.709 q/ha with AquaCrop and 7.703 q/ha with GFDL-ESM2M. Under RCP8.5, yields are 7.765 tons/ha with AquaCrop and 7.198 q/ha with SIMPLE Crop, indicating that reduced emissions could improve wheat growth conditions. Biomass predictions showed significant variation: in Medjez El Beb, average biomass is 17.99 tons/ha with AquaCrop and 18.73 tons/ha with SIMPLE Crop under RCP8.5. In Slougia, average biomass is 18.90 tons/ha with AquaCrop and 19.04 tons/ha with SIMPLE Crop under the same scenario. Growth duration varied, with AquaCrop predicting 175 days in Medjez El Beb and 178 days in Slougia, while SIMPLE Crop predicted 180 days in Medjez El Beb and 182 days in Slougia, with a standard deviation of ±12 days for both models. SIMPLE Crop demonstrated higher accuracy in predicting growth cycle duration and yield, particularly in Slougia, with mean bias errors of −3.6 days and 2.26 q/ha. Conversely, AquaCrop excelled in biomass prediction with an agreement index of 0.97 at Slougia. Statistical analysis revealed significant yield differences based on climate models and emission scenarios, with GFDL-ESM2M under RCP4.5 showing more favorable conditions. These findings emphasize the importance of model selection and calibration for accurately projecting the agricultural impacts of climate change, and they provide insights for enhancing prediction accuracy and informing adaptation strategies for sustainable wheat production in Northwest Tunisia.
{"title":"Future Impact of Climate Change on Durum Wheat Growth and Productivity in Northern Tunisia","authors":"Mohamed Nejib El Melki, Imen Soussi, Jameel Mohammed Al-Khayri, Othman M. Al-Dossary, Bader Alsubaie, Slaheddine Khlifi","doi":"10.3390/agronomy14092022","DOIUrl":"https://doi.org/10.3390/agronomy14092022","url":null,"abstract":"This study evaluates the projected impact of climate change on wheat production in Northwest Tunisia, specifically at Medjez El Beb (36.67 m, 9.74°) and Slougia (36.66 m, 9.6°), for the period 2041–2070. Using the CNRM-CM5.1 and GFDL-ESM2M climate models under RCP4.5 and RCP8.5 scenarios, coupled with the AquaCrop and SIMPLE crop growth models, we compared model outputs with observed data from 2016 to 2020 to assess model performance. The objective was to determine how different climate models and scenarios affect wheat yields, biomass, and growth duration. Under RCP4.5, projected average yields are 7.709 q/ha with AquaCrop and 7.703 q/ha with GFDL-ESM2M. Under RCP8.5, yields are 7.765 tons/ha with AquaCrop and 7.198 q/ha with SIMPLE Crop, indicating that reduced emissions could improve wheat growth conditions. Biomass predictions showed significant variation: in Medjez El Beb, average biomass is 17.99 tons/ha with AquaCrop and 18.73 tons/ha with SIMPLE Crop under RCP8.5. In Slougia, average biomass is 18.90 tons/ha with AquaCrop and 19.04 tons/ha with SIMPLE Crop under the same scenario. Growth duration varied, with AquaCrop predicting 175 days in Medjez El Beb and 178 days in Slougia, while SIMPLE Crop predicted 180 days in Medjez El Beb and 182 days in Slougia, with a standard deviation of ±12 days for both models. SIMPLE Crop demonstrated higher accuracy in predicting growth cycle duration and yield, particularly in Slougia, with mean bias errors of −3.6 days and 2.26 q/ha. Conversely, AquaCrop excelled in biomass prediction with an agreement index of 0.97 at Slougia. Statistical analysis revealed significant yield differences based on climate models and emission scenarios, with GFDL-ESM2M under RCP4.5 showing more favorable conditions. These findings emphasize the importance of model selection and calibration for accurately projecting the agricultural impacts of climate change, and they provide insights for enhancing prediction accuracy and informing adaptation strategies for sustainable wheat production in Northwest Tunisia.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The steady increase in temperature due to global warming can significantly impact weed growth. This study investigates the response of the yellow foxtail (Setaria pumila (Poir.) Roem. & Schult.) grass weed and the Palmer amaranth (Amaranthus palmeri S. Watson) broadleaf weed to increasing temperature conditions, simulating future climate conditions. Temperature treatments included growing these weeds in three temperature-controlled growth chambers: control or ambient conditions (15.5/12.8 °C day/night), moderately elevated temperatures (17.2/14.4 °C), and elevated temperatures (18.8/16.1 °C). Monthly adjustments in the growth chambers simulated the natural temperature rise observed from April to June in the Southern USA, aligning with the respective treatments. Different plant parameters recorded included days to emergence, number of tillers/leaves, chlorophyll content, days to first inflorescence, biomass, and seed set. One-way ANOVA indicated a significant temperature impact on all the parameters assessed (p < 0.05), except for biomass (for both weeds) and days to first inflorescence and yield (for Palmer amaranth (p > 0.05)). The average days to emergence were the lowest under elevated temperatures (8 days for yellow foxtail and 11 days for Palmer amaranth) when compared to that for the control (10 days for yellow foxtail and 19 days for Palmer amaranth). By week 5, yellow foxtail exhibited notably greater tiller numbers under elevated temperatures compared to that of the control; a similar trend was noticed regarding the number of Palmer amaranth leaves. The average chlorophyll content was the highest under elevated temperature conditions up to week 6 and began decreasing after that for both weeds. The average yield of yellow foxtail under elevated, moderately elevated, and control temperatures was 7.55, 2.69, and 0.88 g, respectively. Even though not significant, the yield of Palmer amaranth was higher under elevated temperature conditions as compared to that under the ambient condition. The biomass of both yellow foxtail and Palmer amaranth were not significantly impacted by temperature (p > 0.05). Our research shows that as temperatures rise, weeds exhibit more vigorous growth and show higher photosynthetic efficiency, which has important implications for how we manage weeds in agriculture. These findings suggest that under warmer conditions, weeds could display more vigorous vegetative growth, thus significantly impacting crop yields. As we face ongoing global warming, it is crucial to consider how temperatures influences weed growth when designing strategies to manage weeds effectively.
{"title":"Effect of Elevated Temperature on Growth Parameters and Seed Set of Setaria pumila (Yellow Foxtail) and Amaranthus palmeri (Palmer amaranth) in Southern USA","authors":"Amarnadh Oleti, Amna Dar, Sanju Shrestha, Grishma Ojha, Te-Ming Tseng, Swati Shrestha","doi":"10.3390/agronomy14092025","DOIUrl":"https://doi.org/10.3390/agronomy14092025","url":null,"abstract":"The steady increase in temperature due to global warming can significantly impact weed growth. This study investigates the response of the yellow foxtail (Setaria pumila (Poir.) Roem. & Schult.) grass weed and the Palmer amaranth (Amaranthus palmeri S. Watson) broadleaf weed to increasing temperature conditions, simulating future climate conditions. Temperature treatments included growing these weeds in three temperature-controlled growth chambers: control or ambient conditions (15.5/12.8 °C day/night), moderately elevated temperatures (17.2/14.4 °C), and elevated temperatures (18.8/16.1 °C). Monthly adjustments in the growth chambers simulated the natural temperature rise observed from April to June in the Southern USA, aligning with the respective treatments. Different plant parameters recorded included days to emergence, number of tillers/leaves, chlorophyll content, days to first inflorescence, biomass, and seed set. One-way ANOVA indicated a significant temperature impact on all the parameters assessed (p < 0.05), except for biomass (for both weeds) and days to first inflorescence and yield (for Palmer amaranth (p > 0.05)). The average days to emergence were the lowest under elevated temperatures (8 days for yellow foxtail and 11 days for Palmer amaranth) when compared to that for the control (10 days for yellow foxtail and 19 days for Palmer amaranth). By week 5, yellow foxtail exhibited notably greater tiller numbers under elevated temperatures compared to that of the control; a similar trend was noticed regarding the number of Palmer amaranth leaves. The average chlorophyll content was the highest under elevated temperature conditions up to week 6 and began decreasing after that for both weeds. The average yield of yellow foxtail under elevated, moderately elevated, and control temperatures was 7.55, 2.69, and 0.88 g, respectively. Even though not significant, the yield of Palmer amaranth was higher under elevated temperature conditions as compared to that under the ambient condition. The biomass of both yellow foxtail and Palmer amaranth were not significantly impacted by temperature (p > 0.05). Our research shows that as temperatures rise, weeds exhibit more vigorous growth and show higher photosynthetic efficiency, which has important implications for how we manage weeds in agriculture. These findings suggest that under warmer conditions, weeds could display more vigorous vegetative growth, thus significantly impacting crop yields. As we face ongoing global warming, it is crucial to consider how temperatures influences weed growth when designing strategies to manage weeds effectively.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092021
Víctor Soto-Aquino, Severo Ignacio-Cárdenas, Anghelo Jhosepp Japa-Espinoza, Ulda Campos-Félix, Juanita Ciriaco-Poma, Alex Campos-Félix, Benancio Pantoja-Medina, Juan Z. Dávalos-Prado
In this work, the inter-relationship among 10 morphological characters, 8 climatic parameters and the content of total anthocyanins in the cob core of PMV 581 purple maize, cultivated and produced in three different places in Huanuco–Peru region, has been reported. This study of morphological characters was carried out using standard descriptors, both for the plant and the cob. Data on climatic parameters were obtained from three meteorological stations near the test locations. The total anthocyanin content (Acy), expressed as the glucoside-3-cyanidin concentration, has been determined by the differential pH method. From the statistical treatment of the data obtained, the following descriptors were found to be the most representative, given that they are poorly correlated with each other, but in general, depending on the localities: i/ (morphological) grain weight per cob GWC, plant length PL and cob core weight CCW; ii/ (climatic) minimum temperature Tmin, wind speed v and relative humidity RH. Between both types of descriptors, the best correlations occur for (CCW vs. Tmin) and (GWC vs. v). On the other hand, the total anthocyanin content Acy correlates very well with the CCW and Tmin descriptors. So, the highest concentration of Acy (684.2 mg/100 g) and also the highest CCW (38.6 g/cob) have been obtained in cobs of Winchuspata (W-Q), the coldest (Tmin = 7.89 °C) of the considered localities. On the contrary, the lowest concentration of Acy (603.7 mg/100 g) and also the lowest CCW 25.4 g/cob) have been obtained in cobs of Pistaloli (P-SA), the warmest (Tmin = 19.96 °C) of the three locations. The highest GWC value (139.4 g/cob) has been obtained in cobs of Marabamba (M-Y) where the wind speed v (4.13 m/s) was the highest of the locations considered. On the contrary, the lowest value of GWC (79.6 g/cob) has been obtained for cobs of Pistaloli (P-SA) where v was the lowest (1.19 m/s). In this context, it is important to propose studies on climatic variations’ impact on different crop cycles, investigating how different agronomic management practices and the use of genetic identification/expression tools can optimize the anthocyanin production of purple maize, in order to facilitate the selection of new varieties for specific climatic conditions.
{"title":"Influence of Climatic Parameters and Plant Morphological Characters on the Total Anthocyanin Content of Purple Maize (Zea mays L., PMV-581) Cob Core","authors":"Víctor Soto-Aquino, Severo Ignacio-Cárdenas, Anghelo Jhosepp Japa-Espinoza, Ulda Campos-Félix, Juanita Ciriaco-Poma, Alex Campos-Félix, Benancio Pantoja-Medina, Juan Z. Dávalos-Prado","doi":"10.3390/agronomy14092021","DOIUrl":"https://doi.org/10.3390/agronomy14092021","url":null,"abstract":"In this work, the inter-relationship among 10 morphological characters, 8 climatic parameters and the content of total anthocyanins in the cob core of PMV 581 purple maize, cultivated and produced in three different places in Huanuco–Peru region, has been reported. This study of morphological characters was carried out using standard descriptors, both for the plant and the cob. Data on climatic parameters were obtained from three meteorological stations near the test locations. The total anthocyanin content (Acy), expressed as the glucoside-3-cyanidin concentration, has been determined by the differential pH method. From the statistical treatment of the data obtained, the following descriptors were found to be the most representative, given that they are poorly correlated with each other, but in general, depending on the localities: i/ (morphological) grain weight per cob GWC, plant length PL and cob core weight CCW; ii/ (climatic) minimum temperature Tmin, wind speed v and relative humidity RH. Between both types of descriptors, the best correlations occur for (CCW vs. Tmin) and (GWC vs. v). On the other hand, the total anthocyanin content Acy correlates very well with the CCW and Tmin descriptors. So, the highest concentration of Acy (684.2 mg/100 g) and also the highest CCW (38.6 g/cob) have been obtained in cobs of Winchuspata (W-Q), the coldest (Tmin = 7.89 °C) of the considered localities. On the contrary, the lowest concentration of Acy (603.7 mg/100 g) and also the lowest CCW 25.4 g/cob) have been obtained in cobs of Pistaloli (P-SA), the warmest (Tmin = 19.96 °C) of the three locations. The highest GWC value (139.4 g/cob) has been obtained in cobs of Marabamba (M-Y) where the wind speed v (4.13 m/s) was the highest of the locations considered. On the contrary, the lowest value of GWC (79.6 g/cob) has been obtained for cobs of Pistaloli (P-SA) where v was the lowest (1.19 m/s). In this context, it is important to propose studies on climatic variations’ impact on different crop cycles, investigating how different agronomic management practices and the use of genetic identification/expression tools can optimize the anthocyanin production of purple maize, in order to facilitate the selection of new varieties for specific climatic conditions.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092027
Ning Yang, Xiaoke Wang, Lorenzo Cotrozzi, Cristina Nali, Elisa Pellegrini, Gemma Bianchi, Claudia Pisuttu, Feixiang Zheng
In this work, we assessed the effects of increasing ozone (O3) on four petunia varieties with different floral pigmentation (pink, red, rose-red, and white). Plants were exposed, in open-top chambers located in China, to three O3 concentrations, i.e., (i) ambient air (AA), (ii) AA + 60 ppb O3 (AA + 60), and (iii) AA + 120 ppb O3 (AA + 120), for 85 days (9 h day−1). Flower diameter and duration were assessed, together with leaf chlorophyll and flavonoid contents. White petunia showed a reduced flower diameter and longevity under AA + 60 (−7 and −6%, respectively, in comparison to AA), whereas pink and red petunias only showed this under AA + 120 (−8 and −7%, on average, respectively). Chlorophyll loss occurred in all varieties under AA + 60 (−30%, on average), and at AA + 120 in white and red petunias (−54%, on average). The total flavonoid content in the pink and white varieties increased only under AA + 120 (around +85%), while it grew at both AA + 60 and AA + 120 (+92% and two-fold higher, respectively) in the red variety. Increasing O3 concentrations did not affect particularly the red-rose variety. The white variety showed the strongest correlations among flower and leaf properties, confirming a variety-related O3 response, as well as demonstrating that it had the highest O3 sensitivity.
在这项工作中，我们评估了臭氧（O3）增加对四个不同花色（粉色、红色、玫瑰红和白色）牵牛花品种的影响。在位于中国的敞篷室中，将植物暴露于三种浓度的臭氧中，即(i) 环境空气（AA）、(ii) AA + 60 ppb O3（AA + 60）和(iii) AA + 120 ppb O3（AA + 120），持续 85 天（每天 9 小时）。对花的直径和持续时间以及叶片叶绿素和类黄酮含量进行了评估。在 AA + 60 条件下，白色牵牛花的花径和花期缩短（与 AA 相比，分别缩短了 7% 和 6%），而粉色和红色牵牛花只在 AA + 120 条件下才出现这种情况（平均分别缩短了 8% 和 7%）。在 AA + 60 条件下，所有品种的叶绿素都会减少（平均减少 30%），而在 AA + 120 条件下，白色和红色牵牛花的叶绿素都会减少（平均减少 54%）。粉色和白色品种的总黄酮含量只有在 AA + 120 条件下才有所增加（约 +85%），而红色品种的总黄酮含量在 AA + 60 和 AA + 120 条件下都有所增加（分别增加了 92% 和 2 倍）。O3 浓度的增加对红玫瑰品种的影响并不明显。白色品种的花朵和叶片特性之间的相关性最强，这证实了与品种相关的 O3 反应，同时也表明白色品种对 O3 的敏感性最高。
{"title":"Effects of Increasing Ozone Levels on Leaf Biochemistry and Flower Development in Petunia Varieties with Different Floral Pigmentation","authors":"Ning Yang, Xiaoke Wang, Lorenzo Cotrozzi, Cristina Nali, Elisa Pellegrini, Gemma Bianchi, Claudia Pisuttu, Feixiang Zheng","doi":"10.3390/agronomy14092027","DOIUrl":"https://doi.org/10.3390/agronomy14092027","url":null,"abstract":"In this work, we assessed the effects of increasing ozone (O3) on four petunia varieties with different floral pigmentation (pink, red, rose-red, and white). Plants were exposed, in open-top chambers located in China, to three O3 concentrations, i.e., (i) ambient air (AA), (ii) AA + 60 ppb O3 (AA + 60), and (iii) AA + 120 ppb O3 (AA + 120), for 85 days (9 h day−1). Flower diameter and duration were assessed, together with leaf chlorophyll and flavonoid contents. White petunia showed a reduced flower diameter and longevity under AA + 60 (−7 and −6%, respectively, in comparison to AA), whereas pink and red petunias only showed this under AA + 120 (−8 and −7%, on average, respectively). Chlorophyll loss occurred in all varieties under AA + 60 (−30%, on average), and at AA + 120 in white and red petunias (−54%, on average). The total flavonoid content in the pink and white varieties increased only under AA + 120 (around +85%), while it grew at both AA + 60 and AA + 120 (+92% and two-fold higher, respectively) in the red variety. Increasing O3 concentrations did not affect particularly the red-rose variety. The white variety showed the strongest correlations among flower and leaf properties, confirming a variety-related O3 response, as well as demonstrating that it had the highest O3 sensitivity.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092028
Yonglin Jia, Zhe Xu, Dongliang Zhang, Wei Yang, Yanhong Ding, Changjian Li, Lihua Gao, Kadambot H. M. Siddique, Zhongyi Qu
Biochar is a widely recognized soil amendment used to improve soil physicochemical properties and crop productivity. However, its compositive effects on soil water, fertilizer, and carbon in cropping systems are not extensively understood. A two-year field study was conducted to investigate the influence of integrating maize-residue-derived biochar on evapotranspiration, water and fertilizer utilization efficiency, soil organic carbon (SOC) content, and soil carbon emissions in maize farming, employing drip irrigation in conjunction with film mulching. The treatments included the following three biochar amendments: 15 (B15); 30 (B30); and 45 (B45) t ha−1. Biochar was exclusively incorporated prior to sowing during the initial year of the experiment, and no biochar was used as a control (CK). After two years, the biochar amendments, especially B30, improved crop water productivity and the partial factor productivities of nitrogen, phosphorus, and potassium fertilizer. Relative to CK, the biochar amendments significantly reduced soil CO2 and CH4 emissions during crop growth by 17.6–40.6% and −1.21–268.4%, respectively, averaged across two years. The best treatment effect was achieved by adding 15 t ha−1 of biochar. The biochar needed replenishing in the third year for B30 and in the fourth year for B45 to increase the SOC content by 20 g kg−1. An application rate of 22 metric tons per hectare of biochar is proposed to optimize water and fertilizer utilization efficiency, alongside augmenting the soil organic matter content, within maize farming under drip irrigation and mulching practices on sandy loam soil. However, the biochar must be added at 20 t ha−1 in the third year to counterbalance soil carbon losses and improve SOC.
{"title":"Effects of a Single Biochar Application on Soil Carbon Storage and Water and Fertilizer Productivity of Drip-Irrigated, Film-Mulched Maize Production","authors":"Yonglin Jia, Zhe Xu, Dongliang Zhang, Wei Yang, Yanhong Ding, Changjian Li, Lihua Gao, Kadambot H. M. Siddique, Zhongyi Qu","doi":"10.3390/agronomy14092028","DOIUrl":"https://doi.org/10.3390/agronomy14092028","url":null,"abstract":"Biochar is a widely recognized soil amendment used to improve soil physicochemical properties and crop productivity. However, its compositive effects on soil water, fertilizer, and carbon in cropping systems are not extensively understood. A two-year field study was conducted to investigate the influence of integrating maize-residue-derived biochar on evapotranspiration, water and fertilizer utilization efficiency, soil organic carbon (SOC) content, and soil carbon emissions in maize farming, employing drip irrigation in conjunction with film mulching. The treatments included the following three biochar amendments: 15 (B15); 30 (B30); and 45 (B45) t ha−1. Biochar was exclusively incorporated prior to sowing during the initial year of the experiment, and no biochar was used as a control (CK). After two years, the biochar amendments, especially B30, improved crop water productivity and the partial factor productivities of nitrogen, phosphorus, and potassium fertilizer. Relative to CK, the biochar amendments significantly reduced soil CO2 and CH4 emissions during crop growth by 17.6–40.6% and −1.21–268.4%, respectively, averaged across two years. The best treatment effect was achieved by adding 15 t ha−1 of biochar. The biochar needed replenishing in the third year for B30 and in the fourth year for B45 to increase the SOC content by 20 g kg−1. An application rate of 22 metric tons per hectare of biochar is proposed to optimize water and fertilizer utilization efficiency, alongside augmenting the soil organic matter content, within maize farming under drip irrigation and mulching practices on sandy loam soil. However, the biochar must be added at 20 t ha−1 in the third year to counterbalance soil carbon losses and improve SOC.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092024
Peng Wu, Wei Sun, Hucun Wang, Luhai Zhang, Petru A. Simionescu
To address the challenges of digging deep-rhizome Chinese herbal medicines in northwest China’s hilly terrain, including difficulty, incompleteness, and herb damage, a specialized self-propelled digger with interlocking teeth has been developed. Designed for complex topography, small fields, and resistant soil, this digger provides an efficient and precise alternative to traditional methods. The prototype features in-place reverse differential steering, 360-degree digging capability, and minimized root and soil damage to promote future planting. Key components, including the digging mechanism, vibratory system, crawler chassis, hydraulic transmission system, and worm gear rotary hydraulic reducer, were analyzed and optimized through theoretical, graphical, and simulation studies using RecurDyn. Field tests demonstrated the digger’s effectiveness, achieving depths exceeding 600 mm with minimal herb damage and loss. The digger successfully navigated steep slopes and operated within noise regulations, surpassing industry standards, with less than 1.4% herb damage and a loss rate under 3%. The digger was capable of ascending gradients over 20° with driver noise levels below 92 dB. This innovative solution offers a valuable reference for developing specialized diggers for harvesting traditional Chinese medicinal materials in challenging conditions.
{"title":"Design and Testing of an Inter-Toothed, Self-Propelled Digger for Deep-Rhizome Chinese Herbal Medicines","authors":"Peng Wu, Wei Sun, Hucun Wang, Luhai Zhang, Petru A. Simionescu","doi":"10.3390/agronomy14092024","DOIUrl":"https://doi.org/10.3390/agronomy14092024","url":null,"abstract":"To address the challenges of digging deep-rhizome Chinese herbal medicines in northwest China’s hilly terrain, including difficulty, incompleteness, and herb damage, a specialized self-propelled digger with interlocking teeth has been developed. Designed for complex topography, small fields, and resistant soil, this digger provides an efficient and precise alternative to traditional methods. The prototype features in-place reverse differential steering, 360-degree digging capability, and minimized root and soil damage to promote future planting. Key components, including the digging mechanism, vibratory system, crawler chassis, hydraulic transmission system, and worm gear rotary hydraulic reducer, were analyzed and optimized through theoretical, graphical, and simulation studies using RecurDyn. Field tests demonstrated the digger’s effectiveness, achieving depths exceeding 600 mm with minimal herb damage and loss. The digger successfully navigated steep slopes and operated within noise regulations, surpassing industry standards, with less than 1.4% herb damage and a loss rate under 3%. The digger was capable of ascending gradients over 20° with driver noise levels below 92 dB. This innovative solution offers a valuable reference for developing specialized diggers for harvesting traditional Chinese medicinal materials in challenging conditions.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-05DOI: 10.3390/agronomy14092020
Solange Uwingabire, Shaban Athuman Omar Chamshama, Jean Nduwamungu, Gert Nyberg
Biochar (B) has low nutrient content and is recalcitrant to biodegradation. Supplementing B with a fast-releasing nutrient source may improve soil fertility and physical conditions and increase crop productivity. A three-season field study was conducted on sandy loam and sandy clay loam textured soils to investigate the effect of B mixed with livestock manure (LM) on soil properties (pH, organic carbon (OC), cation exchange capacity (CEC), total Nitrogen (TN), available Phosphorus (Avail P)), and French bean yield (Phaseolus vulgaris L.) in Rwanda. The study used a factorial randomized block design with four replications. Treatments comprised three levels of B (0, 1, and 3 t/ha) and three levels of LM (0, 1, and 3 t/ha). Biochar was used from S. sesban, G. sepium, A. angustissima, Eucalyptus, and Grevillea sp., prepared using a drum kiln, while LM was prepared using the pit method. The Analysis of Variance (ANOVA), Tukey (HSD) function at p < 0.05, and linear mixed-effects model were performed in R software version 4.3.3 (R Core Team, 2024). The analysis showed that the treated plots significantly increased French bean yield compared to the control plots, with the highest value found in plots treated with 3 t/ha. The combined plots showed an increased yield compared to sole Biochar or manure. The seasonal increase has been observed, with percentage increases recorded as follows: 16%, 33.56%, 173.06% in sole B plots; 40.28%, 14.43%, and 11.76% in sole LM plots and 125%, 156%, and 209.8% in B + LM plots for season 1, 2, and 3, respectively. Furthermore, the results indicated that the application of B alone or combined with LM significantly enhanced soil pH, OC, TN, avail P, and CEC with the pH ranging from 6.77 to 5.43 for B alone, 6.7–5.35 for LM alone, 8.53–6.06 for B-LM plots, and 4.34–3.78 for control plots. Applying Biochar, either alone or in combination with LM, at a low rate demonstrated positive effects on French bean yield and soil nutrients in smallholder farmers. This study encourages using natural materials such as B and LM to improve soil fertility and increase vegetable production while reducing chemical fertilizers that can cause pollution and damage the environment.
{"title":"French Bean Production as Influenced by Biochar and Biochar Blended Manure Application in Two Agro-Ecological Zones of Rwanda","authors":"Solange Uwingabire, Shaban Athuman Omar Chamshama, Jean Nduwamungu, Gert Nyberg","doi":"10.3390/agronomy14092020","DOIUrl":"https://doi.org/10.3390/agronomy14092020","url":null,"abstract":"Biochar (B) has low nutrient content and is recalcitrant to biodegradation. Supplementing B with a fast-releasing nutrient source may improve soil fertility and physical conditions and increase crop productivity. A three-season field study was conducted on sandy loam and sandy clay loam textured soils to investigate the effect of B mixed with livestock manure (LM) on soil properties (pH, organic carbon (OC), cation exchange capacity (CEC), total Nitrogen (TN), available Phosphorus (Avail P)), and French bean yield (Phaseolus vulgaris L.) in Rwanda. The study used a factorial randomized block design with four replications. Treatments comprised three levels of B (0, 1, and 3 t/ha) and three levels of LM (0, 1, and 3 t/ha). Biochar was used from S. sesban, G. sepium, A. angustissima, Eucalyptus, and Grevillea sp., prepared using a drum kiln, while LM was prepared using the pit method. The Analysis of Variance (ANOVA), Tukey (HSD) function at p < 0.05, and linear mixed-effects model were performed in R software version 4.3.3 (R Core Team, 2024). The analysis showed that the treated plots significantly increased French bean yield compared to the control plots, with the highest value found in plots treated with 3 t/ha. The combined plots showed an increased yield compared to sole Biochar or manure. The seasonal increase has been observed, with percentage increases recorded as follows: 16%, 33.56%, 173.06% in sole B plots; 40.28%, 14.43%, and 11.76% in sole LM plots and 125%, 156%, and 209.8% in B + LM plots for season 1, 2, and 3, respectively. Furthermore, the results indicated that the application of B alone or combined with LM significantly enhanced soil pH, OC, TN, avail P, and CEC with the pH ranging from 6.77 to 5.43 for B alone, 6.7–5.35 for LM alone, 8.53–6.06 for B-LM plots, and 4.34–3.78 for control plots. Applying Biochar, either alone or in combination with LM, at a low rate demonstrated positive effects on French bean yield and soil nutrients in smallholder farmers. This study encourages using natural materials such as B and LM to improve soil fertility and increase vegetable production while reducing chemical fertilizers that can cause pollution and damage the environment.","PeriodicalId":7601,"journal":{"name":"Agronomy","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142209287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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