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Daya Bunuh Ekstrak Air Daun Salam (Syzygium polyanthum) terhadap Larva Aedes sp
Pub Date : 2017-07-03 DOI: 10.31964/mltj.v3i1.151
Ratih Dewi Dwiyanti, Rion Dediq, Anny Thuraidah
edes sp is known as a mosquito that transmits various diseases. In addition to spreading dengue fever and chikungunya, these mosquitoes also spread the virus zika is quite dangerous. One of the effort to controlling these diseases is to eradicate the vector by using materials that can be taken from plants one of them is bay leaf (Syzygium polyanthum). The purpose of this study is to determine the kills power of Syzygium polyanthum water extract against Aedes sp larvae. This research uses true experiment with Posttest Only With Control Group Design design. Data were obtained from the number of larval deaths in the Syzygium polyanthum extract in each control group and treatment group for 24 hours. The results showed that the percentage of bayleaf water extract which has the largest number of larval deaths was at 32% concentration with 55% death percentage. It was concluded that each concentration of aqueous bay leaf water extract given had a killing power against Aedes sp larvae with the lowest percentage of 2.5% and the highest 55%. Based on the result of Kruskal-Wallis test showed that water extract of the bay leaf was able to kill Aedes sp larvae at each treatment with the value of sig 0,001. Suggestion for the next researcher to test the power of killing the water extract of Syzygium polyanthum to Aedes sp larvae by increasing the concentration of salted bay water extract.
众所周知,伊蚊是一种传播多种疾病的蚊子。除了传播登革热和基孔肯雅热,这些蚊子还传播寨卡病毒是相当危险的。控制这些疾病的努力之一是通过使用可从植物中提取的材料来根除媒介,其中之一是月桂叶(Syzygium polyanthum)。本研究的目的是测定芡实水提物对伊蚊幼虫的杀伤作用。本研究采用单纯后测与对照组设计的真实实验设计。测定各对照组和处理组灌药后24 h的幼虫死亡数。结果表明,月桂叶水提物的幼虫死亡率最高,浓度为32%,死亡率为55%;结果表明,各浓度月桂叶水提物对伊蚊幼虫的杀伤率最低为2.5%,最高为55%。Kruskal-Wallis试验结果表明,月桂叶水提物各处理对伊蚊幼虫的杀伤效果均为0.001。建议后续研究人员通过增加盐海湾水提物的浓度,来测试何参水提物对伊蚊幼虫的杀伤作用。
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引用次数: 3
Pengaruh Variasi Suhu Awal Reagen terhadap Kadar Glukosa Darah Metode Enzimatik 初始温度变化抑制剂Reagen对酶促血糖的抑制作用
Pub Date : 2017-07-03 DOI: 10.31964/mltj.v3i1.147
Yayuk Kustiningsih, Nastiti Megawati, Jasmadi Joko Kartiko, Leka Lutpiatina
Pre-analysis phase is the first step that determines the quality of the examination results, in this case, is the preparation of glucose work reagent. Glucose is one of the clinical examination clinical parameters that are often done in the laboratory. This glucose examination uses enzymatic methods. Enzyme activity is influenced by several factors, namely temperature, pH, substrate level, enzyme level, and inhibitor. Glucose examination performed at a temperature of 20-25oC or 37oC, but there are still laboratories in the field that is less attention to the initial temperature conditions of this reagent. This study aims to determine the effect of initial temperature variations of reagents on blood glucose levels of enzymatic methods. The research used Quasi Experimental research method with Posttest-Only Control Group Design design. The sample of this research used serum of a respondent who had no history of Diabetes Mellitus disease with 4 replication at each treatment. The results of the study with the initial temperature of 10oC, 13oC, 16oC, 19oC, 22oC and 25oC (control) respectively of 62.71 mg / dL; 65,74 mg / dL; 72.45 mg / dL; 76.91 mg / dL; 89.12 mg / dL and 97.19 mg / dL, the results of this study showed an increase in glucose levels in each treatment. Based on the results of linear regression analysis obtained a significance value of 0.000 with α = 0.05, which means Ho is rejected, so it can be concluded that there is an influence of initial temperature variation of reagent on blood glucose level. For the next researcher can do research about the effect of the initial temperature of reagent 22-38 oC on blood glucose enzymatic method. And the influence of incubation time on blood glucose level of enzymatic method.
预分析阶段是决定检查结果质量的第一步,在这种情况下,是葡萄糖工作试剂的制备。葡萄糖是临床检查的临床参数之一,通常在实验室进行。这种葡萄糖检查使用酶法。酶活性受几个因素的影响,即温度、pH、底物水平、酶水平和抑制剂。葡萄糖检测在20-25℃或37℃的温度下进行,但该领域仍有实验室不太关注该试剂的初始温度条件。本研究旨在确定酶法试剂初始温度变化对血糖水平的影响。本研究采用准实验研究方法,采用后验对照组设计设计。这项研究的样本使用了一名没有糖尿病病史的受访者的血清,每次治疗有4次复制。初始温度为10℃、13℃、16℃、19℃、22℃和25℃(对照)的研究结果分别为62.71 mg/dL;65,74 mg/dL;72.45mg/dL;76.91 mg/dL;89.12 mg/dL和97.19 mg/dL,这项研究的结果显示,每次治疗的葡萄糖水平都有所增加。根据线性回归分析的结果,获得了0.000的显著性值,α=0.05,这意味着Ho被拒绝,因此可以得出结论,试剂的初始温度变化对血糖水平有影响。下一步研究人员可以研究试剂初始温度22-38℃对血糖酶法的影响。以及培养时间对酶法血糖水平的影响。
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引用次数: 2
Cemaran Bakteri Gram Negatif pada Jajanan Siomay di Kota Kendari Kendari市Siomay路革兰氏阴性菌Cemaran
Pub Date : 2017-07-03 DOI: 10.31964/MLTJ.V3I1.111
Reni Yunus, Ruth Mongan, Rosnani Rosnani
Foodborne disease caused by food infection is one of health concern which cause of consumption of contaminated foods that containing living microorganisms. There are several kinds of microorganisms that can cause food infection, one of them is Salmonella sp, a negative Gram basil shaped-bacteria as the cause typhoid and paratyphoid diseases. The purpose of this study is isolate and identify negative Gram bacteria on snacks in the traditional market Anduonuhu Kendari. This research is a descriptive study with dumplings samples which sale on the traditional Market Anduonohu as population. Samples were taken as a total sampling with pour plate method was used for bacterial isolation. All 10 samples were shown positive result which growth in Brain Heart Infusion Broth media as 7 of them are positively grow in Salmonella Shigella Agar with negative Gram basil-shaped bacteria was found under the microscope after Gram staining, suspected as negative Gram baceria. It can be concluded that 7 (70%) out of of 10 dumplings samples were contaminated negative Gram bacteria. This research can be continued by identifying other pathogenic bacteria which are the bacteria that cause food borne disease.
食物感染引起的食源性疾病是人们食用含有活微生物的受污染食品所引起的健康问题之一。有几种微生物可以引起食物感染,其中一种是沙门氏菌,一种阴性的革兰氏罗勒形细菌,可引起伤寒和副伤寒疾病。本研究的目的是对传统市场安多诺胡肯达里小吃中阴性革兰氏菌进行分离鉴定。本研究以安多诺胡传统市场上销售的饺子为样本进行描述性研究。样品作为总采样,采用淋板法进行细菌分离。10份样品在脑心灌注肉汤培养基中均呈阳性,其中7份在志贺氏沙门氏菌琼脂培养基中呈阳性,革兰氏染色显微镜下检出革兰氏罗勒形菌阴性,怀疑为革兰氏阴性菌。结果表明,10个饺子样品中有7个(70%)被革兰氏阴性菌污染。这项研究可以通过确定引起食源性疾病的其他致病菌来继续进行。
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引用次数: 6
Kadar Vitamin C Jeruk Sunkist Peras dan Infused Water 维生素C黄丝防晒与吸水率
Pub Date : 2017-07-03 DOI: 10.31964/MLTJ.V3I1.149
Haitami Haitami, Annisa Ulfa, Akhmad Muntaha
Orange is one of the fruits that are widely consumed by the society, besides to the sweet taste and attractive appearance orange also has various benefits for the body such as vitamins and minerals. The famous and much-favored orange is a beneficial Sunkist orange can treat fever, treat infections, improve body immunity and decreasing the number of cholesterol. There are several ways of processing oranges such as orange juice and infused the water. The purpose of this study is to know the ratio of vitamin C content in the juice of Sunkist with infused water orange Sunkist. Types of research is analytic survey with comparative study approach. The research sample used was orange Sunkist which made two treatment that is the juice of Sunkist orange and infused water by using Iodimetry titration method. The results of the average vitamin C content in 10 samples of citrus Sunkist for 348,82 ppm and the average vitamin C level in 10 samples infused water of orange Sunkist equal to 67,59 ppm. Based on statistical test result using Independent Sample T-Test test stated that there is the difference of vitamin C content on freshly squeezed orange Sunkist with infused water of orange Sunkist with the value of significance 0.000. Suggested for further researcher can do research of vitamin C Infused water level by combining with other fruit
橙子是被社会广泛消费的水果之一,除了香甜的味道和诱人的外观,橙子还对身体有多种好处,如维生素和矿物质。著名的广受欢迎的橙子是一种有益的新奇士橙子,可以治疗发烧,治疗感染,提高身体免疫力,降低胆固醇的数量。加工橙子有几种方法,如榨汁和浸水。本研究的目的是了解新奇士果汁中维生素C含量与注入水橙新奇士的比例。研究的类型是分析调查与比较研究的方法。本研究使用的样品为新奇士橙,采用碘量滴定法对新奇士橙汁和冲泡水进行了两种处理。结果显示,10个柑橘类新奇士样品的维生素C平均含量为348.82 ppm, 10个橙类新奇士注入水中的维生素C平均含量为67.59 ppm。采用独立样本t检验的统计检验结果表明,鲜榨橙服与橙服注水的维生素C含量存在差异,其显著性值为0.000。建议进一步研究人员可以结合其他水果进行维生素C注入水平的研究
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引用次数: 7
Analisis Kadar Siklamat pada Es Krim di Kota Banjarbaru Banjarnew市冰淇淋宣传率分析
Pub Date : 2017-07-03 DOI: 10.31964/MLTJ.V3I1.148
Nurlailah Nurlailah, Nurhayati Aslami Alma, Neni Oktiyani
Sweeteners are one of the components which are often added to foodstuffs. Synthetic sweeteners are widely circulated in the community is cyclamate. Consumption of cyclamate that exceeds the dose will lead to bladder cancer. It will also cause lung, liver, and lymph tumors. The purpose of this study was to determine the presence of cyclamate in ice cream that exceeded the required threshold. The type of this research is the descriptive survey. The sample of this research is ice cream that produced by household companies from all ice cream traders in North Banjarbaru with 11 ice cream sellers. The variables in this study were cyclamate content found in ice cream. The results showed that 11 samples of ice cream were examined, 9 samples containing cyclamate with the highest level of 7.37 g / kg as cyclamic acid. The conclusion of this study is ice cream containing cyclamate found as much as 82% (9 samples), while 18% (2 samples) others negative, from 9 samples containing cyclamate positive, 89% did not qualify the requirements of PERMENKES Number 208 in 1985 that exceeds 2 gr/Kg as cyclamate acid. More specific checks are required for the analysis of cyclamate levels by other methods such as High Performance Liquid Chromatography.
甜味剂是经常添加到食品中的成分之一。合成甜味剂在社区中广泛流通的是甜蜜素。甜蜜素的消费超过剂量将导致膀胱癌症。它还会引起肺、肝和淋巴肿瘤。这项研究的目的是确定冰淇淋中甜蜜素的含量是否超过了要求的阈值。这项研究的类型是描述性调查。这项研究的样本是由家庭公司从北班珠巴鲁的所有冰淇淋贸易商和11名冰淇淋销售商那里生产的冰淇淋。这项研究的变量是冰淇淋中的甜蜜素含量。结果表明,对11份冰淇淋样品进行了检测,其中9份样品含有甜蜜素,最高含量为7.37克/公斤的甜蜜素酸。这项研究的结论是,含有甜蜜素的冰淇淋中发现的甜蜜素含量高达82%(9个样品),而其他18%(2个样品)为阴性,在含有甜蜜素阳性的9个样品中,89%不符合1985年第208号PERMENKES的要求,即超过2克/公斤的甜蜜素酸。通过高效液相色谱法等其他方法分析甜蜜素水平需要进行更具体的检查。
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引用次数: 4
Tinjauan Kualitas Bakteriologis dan Tingkat Risiko Pencemaran Air Sumur Gali di Kelurahan Sungai Ulin Kota Banjarbaru Ulin-Banjarnew河加利西亚水质调查及水污染源风险
Pub Date : 2017-07-03 DOI: 10.31964/MLTJ.V3I1.150
Erpan Roebiakto, Gunung Setiadi, Yohannes Joko Supriyadi
Humans in meeting the needs of water should pay attention to aspects of quality and quantity. Particularly the aspect of bacteriological quality, because contaminated water can cause waterborne disease. One of the clean water facilities used by the community is the well. The community of Sungai Ulin Kota Banjarbaru uses wells for daily activities. The purpose of this research is to know the bacteriological quality and the level of risk of water pollution wells Sungai Ulin Kelurahan Kota Banjarbaru. The research type is descriptive and cross-sectional research design. The population of this study was all wells in three Rukun Tetangga (RT 19, RT 20 and RT 21) of 40 and samples of bacteriological quality of well good water. The result of the research is the characteristic of respondent from the level of education of most elementary school is 19 (47.5%), knowledge level 21 (52,5%), middle income category (1-3 million / month) 36 (90%) and length of stay Respondents old category (> 10 years) as many as 39 (78%). The conclusion of the study showed that the quality of bacteriologic (MPN Coli) of well water 3 (7.5%) was eligible and 37 (92.5%) were not eligible. The level of risk of contamination of wells to 40 wells, among others, for very high risk of 6 units (15%), high risk of 26 units (65%), medium risk 5 pieces (12.5%) and low risk 3 (7.5%). Need to do research about the relationship of good construction with bacteriological quality of good water.
人类在满足对水的需求时应注意质量和数量方面的问题。特别是在细菌质量方面,因为受污染的水会引起水传播疾病。该社区使用的清洁水设施之一是水井。Sungai Ulin Kota Banjarbaru社区使用水井进行日常活动。本研究的目的是了解Sungai Ulin Kelurahan Kota Banjarbaru水井的细菌质量和水污染风险水平。研究类型为描述性和横断面研究设计。本研究的人群是Rukun Tetangga三口井(RT 19、RT 20和RT 21)的所有井(共40口)和井水的细菌质量样本。研究结果表明,大多数小学受教育程度的受访者为19人(47.5%),知识水平的受访者为21人(52.5%),中等收入类别(1-3百万/月)的受访者为36人(90%),而老年类别(>10年)的受访者则多达39人(78%)。研究结论表明,3号井水(7.5%)的细菌质量合格,37号井水(92.5%)的细菌不合格。40口井的污染风险水平,包括6口井的极高风险(15%)、26口井的高风险(65%)、5口井的中等风险(12.5%)和3口井的低风险(7.5%)。需要研究良好施工与良好水质的细菌质量之间的关系。
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引用次数: 0
Daya Hambat NaCl terhadap Pertumbuhan Staphylococcus aureus NaCl对葡萄球菌生长的抑制能力
Pub Date : 2016-12-31 DOI: 10.31964/MLTJ.V2I2.125
A. Amalia, Ratih Dewi Dwiyanti, Haitami Haitami
Staphylococcus aureus is a pathogenic bacterial that causes infectious disease and human poisoning through enterotoxin produced by the bacteria. Salt or sodium chloride is used by human for food preservation process because it can inhibit the growth of bacteria, especially Staphylococcus aureus. The purposes of the study were to determine the effect of NaCl in media on the growth of Staphylococcus aureus and determine the effective concentration of NaCl. The type of research used in this study was true experiment posttest only control group design. Samples were prepared in 5 NaCl concentrations, they were 10%, 15%, 20%, 25% dan 30%. The study conclusion was there were effects of NaCl addition to the media on the growth of Staphylococcus aureus with a significance value of 0.000 (<0.05). The effective NaCl concentration that inhibited the growth of Staphylococcus aureus is 15%. The advice for next study is to conduct similar studies with NaCl concentration of 10% - 15%.
金黄色葡萄球菌是一种致病细菌,通过细菌产生的肠毒素引起传染病和人类中毒。由于盐或氯化钠能抑制细菌,尤其是金黄色葡萄球菌的生长,因此被人类用于食品保鲜。本研究的目的是确定培养基中NaCl对金黄色葡萄球菌生长的影响,确定NaCl的有效浓度。本研究采用的研究类型为真正的实验后测纯对照组设计。NaCl浓度分别为10%、15%、20%、25%和30%。研究结论:培养基中添加NaCl对金黄色葡萄球菌的生长有影响,显著值为0.000(<0.05)。抑制金黄色葡萄球菌生长的有效NaCl浓度为15%。下一步的研究建议是在NaCl浓度为10% - 15%的情况下进行类似的研究。
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引用次数: 11
Kesesuaian Hasil Pemeriksaan RT PCR, RDT NS1, dan RDT IgM Pasien Penyakit Dengue 经RT PCR、RDT NS1和登革热病患者检测结果一致
Pub Date : 2016-12-31 DOI: 10.31964/mltj.v2i2.97
Paisal Paisal, Mukhlis Zuardi, R. Herman
The incidence of dengue disease in the world is estimated at 390 million cases per year. In Indonesia, during 2013 there were 35-40 cases per 100.000 population, with a mortality rate of 0.73%. This study aimed to determine the suitability and the percentage of RT-PCR, RDT NS1, and RDT IgM detection examination. Samples were obtained from hospitals in Aceh province during 2012. The research samples reached 100 collected samples, it was only 82 samples that fulfill the analysis criteria. Cohen’s Kappa test result showed there was moderate suitability between RT-PCR and RDT NS1 (K=0,404, p = 0,000), and weak suitability between RT-PCR began RDT IgM (K=0,139, p = 0,046). While the percentage of detection for RT-PCR, RDT NS1, dan RDT IgM were 16%, 10%, and 60%. RDT IgM is the best alternative for laboratory examination in the hospital.
全世界登革热的发病率估计为每年3.9亿例。在印度尼西亚,2013年每10万人中有35-40例病例,死亡率为0.73%。本研究旨在确定RT-PCR、RDT NS1和RDT IgM检测检查的适用性和百分比。2012年期间从亚齐省的医院获得了样本。研究样本达到100个采集样本,只有82个样本符合分析标准。Cohen’s Kappa检验结果显示,RT-PCR与RDT NS1的适宜性中等(K=0,404, p = 0,0000),而RT-PCR与RDT IgM的适宜性较弱(K=0,139, p = 0,046)。而RT-PCR、RDT NS1和RDT IgM的检出率分别为16%、10%和60%。RDT IgM是医院实验室检查的最佳选择。
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引用次数: 2
Kandungan Asam Oksalat Sayur Bayam 菠菜甘蓝酸含量
Pub Date : 2016-12-31 DOI: 10.31964/MLTJ.V2I2.95
Herlena Fitriani, Nurlailah Nurlailah, Dinna Rakhmina
Spinach is one of vegetable that is often used as processed food by the people of Indonesia. Besides containing many nutrients, spinach also contains chemical compounds that are negative, that is oxalic acid. Oxalic acid and its salts are water soluble that can be harmful because these compounds are toxic. This study aimed to determine differences in levels of oxalic acid in spinach when the water is allowed to stand at room temperature. The type of research was pre-experiment with one group pretest-posttest design. Samples of spinach water were divided into 4 treatment and the level of oxalic acid was examined by using permanganometry titration method. Results of research on each treatment showed oxalic acid levels on 0 hour standing was 3753.2 mg/L, 2 hours standing was 3980.0 mg/L, 4 hours standing was 4066.5 mg/L, and the 6 hours standing was 4254.5 mg/L. Repeated ANOVA statistical test results stated there were significant differences in the levels of oxalic acid in spinach water between 0 hour standing and room temperature-standing with a significance value of p <0.05. It is concluded that there are significant differences in the levels of oxalic acid in spinach water between 0 hour standing and room temperature-standing. It is advisable to continue the research by comparing the levels of oxalic acid in spinach with different types, such as green spinach, white spinach, and red spinach.
菠菜是一种经常被印度尼西亚人用作加工食品的蔬菜。除了含有许多营养物质外,菠菜还含有有害的化合物,那就是草酸。草酸及其盐是水溶性的,可能是有害的,因为这些化合物是有毒的。这项研究旨在确定菠菜中草酸水平的差异,当水被允许在室温下放置时。研究类型为预实验,采用一组前测后测设计。将菠菜水分为4个处理,采用高锰酸钾滴定法检测草酸的含量。各处理的草酸浓度在静置0 h时为3753.2 mg/L,静置2 h时为3980.0 mg/L,静置4 h时为4066.5 mg/L,静置6 h时为4254.5 mg/L。重复方差分析(ANOVA)统计检验结果显示,菠菜水中草酸含量在静置0小时与室温静置期间差异显著,显著值p <0.05。综上所述,菠菜水中草酸含量在静置0 h和室温静置期间存在显著差异。我们建议继续研究,比较不同种类的菠菜,如绿菠菜、白菠菜和红菠菜中草酸的含量。
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引用次数: 6
Peran Ekstrak Daun Pepaya (Carica papaya Linn) terhadap Kematian Larva Nyamuk Aedes aegypti 番木瓜对抗埃及伊蚊幼虫死亡
Pub Date : 2016-12-31 DOI: 10.31964/MLTJ.V2I2.98
D. Swastika, Lenie Marlinae, Laily Khairiyati
Papaya plants contain many substances called papain, papain protease effect can kill larvae of Aedes aegypti. This study aimed to determine the effect of papaya leaves extracts (Carica papaya Linn) against Aedes aegypti larvae mortality. The research method was a quasi-experimental research design with Non-Equivalent Control Group. The concentration levels were 6.25%, 12.5%, 25%, 50%, 100%, and controls (CMC-Na) with 4 times replication. The Probit Analysis test results showed Lethal concentration (LC50) of papaya leaves extract (Carica papaya Linn) against Aedes aegypti larvae mortality was to 24.46% and the effective time is 22 hours. It is concluded that any different concentrations of papaya (Carica papaya Linn) leaves extract have effect on Aedes aegypti larvae mortality (p <0.05), the time depth of papaya leaves extract have effect on Aedes aegypti larvae mortality (p <0.05), the average number of Aedes aegypti larvae mortality for 24-hours in each concentration are concentration of 6.25% as much as 4.75 larvae, 12.5% concentration as much as 8 larvae, a concentration of 25% as much as 12 larvae, 50% concentration of 14.5 larvae, concentration 100% as much as 19 larvae and control as much as 1.25 larvae. The recommended suggestion is to conduct a phytochemical test to determine the active substances contained in papaya (Carica papaya Linn) leaves extract and to know the concentration of active substances in the extract.
木瓜植物中含有许多叫做木瓜蛋白酶的物质,木瓜蛋白酶能杀死埃及伊蚊的幼虫。本研究旨在研究木瓜叶提取物(Carica papaya Linn)对埃及伊蚊幼虫死亡率的影响。研究方法采用非等效对照组的准实验研究设计。浓度水平分别为6.25%、12.5%、25%、50%、100%和对照(CMC-Na),重复4次。Probit分析结果表明,番木瓜叶提取物对埃及伊蚊幼虫的致死浓度(LC50)为24.46%,有效时间为22 h。结果表明,不同浓度番木瓜叶提取物对埃及伊蚊幼虫的死亡率均有影响(p <0.05),番木瓜叶提取物的时间深度对埃及伊蚊幼虫的死亡率均有影响(p <0.05),各浓度下埃及伊蚊幼虫24小时平均死亡率分别为6.25%浓度最多4.75只、12.5%浓度最多8只、25%浓度最多12只;50%浓度可达14.5只幼虫,100%浓度可达19只幼虫,对照可达1.25只幼虫。建议进行植物化学试验,测定木瓜叶提取物中含有的活性物质,并了解提取物中活性物质的浓度。
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引用次数: 3
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Medical laboratory technology
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