Urease is an enzyme catalyst in the reaction of oxidation of uric acid into Allantoin. Oxidation of uric acid by the enzyme has become a basic principle of measurement of uric acid levels in the human body. This research to know urikase derived from the bacterium Bacillus sp that contaminate the air Laboratory of Microbiology Department of Health Analyst Banjarmasin. The research is descriptive survey. Airborne bacteria were isolated and identified for Bacillus sp. The identification is done by examination of macroscopic, microscopic and biochemical tests subsequently performed tests using nutrient agar medium containing 0.2% of uric acid. Results of the study showed there were two isolates of Bacillus sp and obtained clear zone on both these isolates. Conclusions of research there are contaminants Bacillus sp. as much as 40% in the laboratory. 100% of the yield urikase Bacillus sp. Urikase advice from Bacillus sp can be used as an alternative reagent uric acid probes spectophotometric method.
{"title":"Produk Urikase dari Bacillus sp. Kontaminan Laboratorium","authors":"Leka Lutpiatina","doi":"10.31964/MLTJ.V1I2.22","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.22","url":null,"abstract":"Urease is an enzyme catalyst in the reaction of oxidation of uric acid into Allantoin. Oxidation of uric acid by the enzyme has become a basic principle of measurement of uric acid levels in the human body. This research to know urikase derived from the bacterium Bacillus sp that contaminate the air Laboratory of Microbiology Department of Health Analyst Banjarmasin. The research is descriptive survey. Airborne bacteria were isolated and identified for Bacillus sp. The identification is done by examination of macroscopic, microscopic and biochemical tests subsequently performed tests using nutrient agar medium containing 0.2% of uric acid. Results of the study showed there were two isolates of Bacillus sp and obtained clear zone on both these isolates. Conclusions of research there are contaminants Bacillus sp. as much as 40% in the laboratory. 100% of the yield urikase Bacillus sp. Urikase advice from Bacillus sp can be used as an alternative reagent uric acid probes spectophotometric method.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"96-101"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vitisvinifera is one of the perishable fruit and not durable when stored at room temperature. To maintain the levels of vitamin C wine then used treatment using calcium chloride (CaCl2). This study aims to determine the effect of treatment Calcium chloride (CaCl2) and duration of storage of the vitamin C content of Vitisvinifera This type of research uses experimental methods actual (true experiment) in the form of draft posttest only control group design. Wine and CaCl2 research materials. Data were obtained by performing assays using titration iodometry vitamin C in grapes. Results of statistical analysis to test multiple linear regression R2 values obtained showed no effect of treatment 0.578 Calcium chloride (CaCl2) and storage time for blood levels of vitamin C Vitisvinifera. From Duncan statistical test obtained significant value for all subsets of the 2 treatment groups of more than 0.05, the most optimal levels of vitamin C in maintaining the Vitisvinifera is a CaCl2 concentration of 0.05 M for 4 days of storage. Therefore, this study suggested as alternative preservation for merchants and producers / grape growers in order to maintain the vitamin C content of wine so as to improve the quality and the quality of the fruit.
{"title":"Pengaruh Kalsium Klorida (CaCl2) dan Lama Penyimpanan terhadap Kadar Vitamin C Anggur (Vitisvinifera)","authors":"Anny Thuraidah, Haitami Haitami, Akhmad Dairobi","doi":"10.31964/MLTJ.V1I2.18","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.18","url":null,"abstract":"Vitisvinifera is one of the perishable fruit and not durable when stored at room temperature. To maintain the levels of vitamin C wine then used treatment using calcium chloride (CaCl2). This study aims to determine the effect of treatment Calcium chloride (CaCl2) and duration of storage of the vitamin C content of Vitisvinifera This type of research uses experimental methods actual (true experiment) in the form of draft posttest only control group design. Wine and CaCl2 research materials. Data were obtained by performing assays using titration iodometry vitamin C in grapes. Results of statistical analysis to test multiple linear regression R2 values obtained showed no effect of treatment 0.578 Calcium chloride (CaCl2) and storage time for blood levels of vitamin C Vitisvinifera. From Duncan statistical test obtained significant value for all subsets of the 2 treatment groups of more than 0.05, the most optimal levels of vitamin C in maintaining the Vitisvinifera is a CaCl2 concentration of 0.05 M for 4 days of storage. Therefore, this study suggested as alternative preservation for merchants and producers / grape growers in order to maintain the vitamin C content of wine so as to improve the quality and the quality of the fruit.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"61-71"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69806904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pre-analytical phases is a very important stage and need to be considered properly. Pre-analytical phases of which is the process of blood sampling, sample delivery, the inclusion of the type of inspection, sample preparation and selection tools. A fact which still often the case that their neglect by nurses or laboratory personnel in taking and processing the blood samples. Blood samples for examination platelet counts as much as possible is done properly and the sample must be examined in less than 1 hour after taking blood. Delays checks can cause a decrease in platelet count. Delays often occur for over an hour due to the shipment of samples from wards that are not immediately performed or work shift lab personnel. The aim of research to determine the difference in the number of platelets in the blood EDTA is immediately checked and a delay of 1 hour by using KX-21 Hematology analyzer. Experimental study design with pre- and post-test study without control. The study was conducted at the Laboratory of RSJ Grhasia Yogyakarta, with the object of research is venous blood from patients RSJ Grhasia between the ages of 20-50 years of both men - men and women. Data were analyzed parametric statistical tests Paired samples t-test. The results of significant research value of the results of parametric statistical tests Paired samples t-test was 0,000 (sig <0.05). There is a difference between the number of blood platelets EDTA is immediately checked and a delay of 1 hour.
{"title":"Perbedaan Jumlah Trombosit Pada Darah EDTA Yang Segera Diperiksa dan Penundaan Selama 1 Jam di Laboratorium RSJ Grhasia Yogyakarta","authors":"Sujud Sujud, ratih hardiasari, Anik Nuryati","doi":"10.31964/MLTJ.V1I2.21","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.21","url":null,"abstract":"Pre-analytical phases is a very important stage and need to be considered properly. Pre-analytical phases of which is the process of blood sampling, sample delivery, the inclusion of the type of inspection, sample preparation and selection tools. A fact which still often the case that their neglect by nurses or laboratory personnel in taking and processing the blood samples. Blood samples for examination platelet counts as much as possible is done properly and the sample must be examined in less than 1 hour after taking blood. Delays checks can cause a decrease in platelet count. Delays often occur for over an hour due to the shipment of samples from wards that are not immediately performed or work shift lab personnel. The aim of research to determine the difference in the number of platelets in the blood EDTA is immediately checked and a delay of 1 hour by using KX-21 Hematology analyzer. Experimental study design with pre- and post-test study without control. The study was conducted at the Laboratory of RSJ Grhasia Yogyakarta, with the object of research is venous blood from patients RSJ Grhasia between the ages of 20-50 years of both men - men and women. Data were analyzed parametric statistical tests Paired samples t-test. The results of significant research value of the results of parametric statistical tests Paired samples t-test was 0,000 (sig <0.05). There is a difference between the number of blood platelets EDTA is immediately checked and a delay of 1 hour.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"91-95"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Efforts to efficiency in the implementation of the medical laboratory's internal quality control is done with the use of control serum pool. Pool control serum is a material made from leftover serum of patients who generally discarded because it is not used anymore .. The purpose of the study to determine the accuracy of the quality of the pool serum as compared with the control materials for the plant control serum glucose in improving internal quality control. Type analytic survey research with comparative approach. Analysis of data using the value of CV (coefficient of variation) and the Wilcoxon test. The results showed a decrease in serum pool of blood glucose levels after 30 days of storage by 4.7%. Based on a statistical test serum taken with the pool serum obtained significance value of 0.000 where this value is less than the value of α = 0.05, which means that there is a difference between serum taken with the pool serum. In the control serum before and after storage decreased blood glucose levels of 4.5% and is based on statistical test obtained significance value of 0.073 where the value is more than the value of α = 0.05, so there is no difference between the control serum before and after storage. Based on the calculation, the value of CV (coefficient of variation) of 5.4% serum pool and a CV (coefficient of variation) control serum by 11.6%. Accuracy pool serum and serum control exceeds the limits CV (coefficient of variation) a maximum of 5%. It is suggested further research on how long endurance pool serum as a control.
{"title":"Pemanfaatan Pool Serum sebagai Bahan Kontrol Ketelitian Pemeriksaan Glukosa Darah","authors":"M. Muslim, Yayuk Kustiningsih, Endah Yanuarti","doi":"10.31964/MLTJ.V1I2.17","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.17","url":null,"abstract":"Efforts to efficiency in the implementation of the medical laboratory's internal quality control is done with the use of control serum pool. Pool control serum is a material made from leftover serum of patients who generally discarded because it is not used anymore .. The purpose of the study to determine the accuracy of the quality of the pool serum as compared with the control materials for the plant control serum glucose in improving internal quality control. Type analytic survey research with comparative approach. Analysis of data using the value of CV (coefficient of variation) and the Wilcoxon test. The results showed a decrease in serum pool of blood glucose levels after 30 days of storage by 4.7%. Based on a statistical test serum taken with the pool serum obtained significance value of 0.000 where this value is less than the value of α = 0.05, which means that there is a difference between serum taken with the pool serum. In the control serum before and after storage decreased blood glucose levels of 4.5% and is based on statistical test obtained significance value of 0.073 where the value is more than the value of α = 0.05, so there is no difference between the control serum before and after storage. Based on the calculation, the value of CV (coefficient of variation) of 5.4% serum pool and a CV (coefficient of variation) control serum by 11.6%. Accuracy pool serum and serum control exceeds the limits CV (coefficient of variation) a maximum of 5%. It is suggested further research on how long endurance pool serum as a control.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69806888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hypercholesterolemia is an excess of cholesterol in the blood, can be factors for heart disease and stroke. The cause of hypertension is heredity, age, sex, obesity (overeating), lack of exercise, stress, excessive salt intake, another effect: smoking, alcohol consumption, taking drugs. The purpose of this study was to determine the relationship of fiber intake, cholesterol, sodium and exercise with cholesterol and hypertension in the elderly group S. Parman Banjarmasin. Type of research is descriptive analytic research using cross sectional design of the study, which was conducted in a group of elderly Regional Health Center S. Parman Banjarmasin. This research was conducted in January through June 2015 with a sample size of 50 people. The data obtained is the intake of cholesterol, sodium, exercise and cholesterol and hypertension. The statistical test used is Chi Square test (p <0.005). The results of this study are most of the respondents were female (68%), most of the respondents aged less than 60 years (56%), and respondents work mostly does not work / housewife (48%). Most fiber intake respondents are not good (96%), cholesterol intake was mostly good (82%), sodium intake are all good (100%), most of the sports activities of respondents are less good (88%), there was no association between dietary fiber intake and kolestero cholesterol levels and hypertension, there is no relationship between exercise activities cholesterol levels and hypertension
{"title":"Hubungan Asupan Serat, Kolesterol, Natrium dan Olahraga Dengan Kadar Kolesterol dan Hipertensi pada Lansia","authors":"Magdalena Magdalena","doi":"10.31964/MLTJ.V1I2.16","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.16","url":null,"abstract":"Hypercholesterolemia is an excess of cholesterol in the blood, can be factors for heart disease and stroke. The cause of hypertension is heredity, age, sex, obesity (overeating), lack of exercise, stress, excessive salt intake, another effect: smoking, alcohol consumption, taking drugs. The purpose of this study was to determine the relationship of fiber intake, cholesterol, sodium and exercise with cholesterol and hypertension in the elderly group S. Parman Banjarmasin. Type of research is descriptive analytic research using cross sectional design of the study, which was conducted in a group of elderly Regional Health Center S. Parman Banjarmasin. This research was conducted in January through June 2015 with a sample size of 50 people. The data obtained is the intake of cholesterol, sodium, exercise and cholesterol and hypertension. The statistical test used is Chi Square test (p <0.005). The results of this study are most of the respondents were female (68%), most of the respondents aged less than 60 years (56%), and respondents work mostly does not work / housewife (48%). Most fiber intake respondents are not good (96%), cholesterol intake was mostly good (82%), sodium intake are all good (100%), most of the sports activities of respondents are less good (88%), there was no association between dietary fiber intake and kolestero cholesterol levels and hypertension, there is no relationship between exercise activities cholesterol levels and hypertension","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"47-53"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69806833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Examination of hemoglobin levels influenced leukocytosis sianmethemoglobin method that causes increased absorbance measurements of hemoglobin levels increased significantly and the false blood sample that has been diluted with a solution Drabkins in centrifugation at 3000 rpm for 10 minutes and then the absorbance of the supernatant was measured with a photometer at λ 546 nm. This study aimed to analyze the differences in hemoglobin level examination siamethemoglobin method with and without centrifugation at sample leukocytosis. This type of research is observational research laboratory. The study design was cross-sectional study. Samples were taken from the remaining blood samples of patients who have been examined leukositnya number more than 20,000 / uL with Hematology Analyzer (CEL-DYN Ruby) February-April 2014, and were divided into 4 groups based on criteria that group 1. leukocyte count of 20,000 / uL-29 999 / mL, group II. 30,000 / uL-39 999 / uL, the group III. 40,000 / uL-49,999 / uL, the group IV. More than 50,000 / uL. The number of samples taken were 20 samples of each group, a total sample of 80 samples. The analysis showed no significant difference in hemoglobin levels siamethemoglobin method with and without centrifugation at sample leukocytosis with a value of p = 0.000 less than 0.05 α. Leukocytosis Turbidity affects the difference in hemoglobin levels with and without centrifugation, the higher the number the greater the difference in leukocyte levels of hemoglobin, hemoglobin level examination results of the study based on the criteria of the number of leukocytes obtained by the difference in hemoglobin levels with and without centrifugation in group I. 0.22 ± 0.07 g / dL, group II 0.40 ± 0.22 g / dL, a group III. 0.44 ± 0.14 g / dL, Group IV. 0.85 ± 0.41 g / dL. The level of hemoglobin in the sample sianmethemoglobin method leukocytosis with more than 20,000 / uL need a centrifuge so that appropriate hemoglobin levels over the patient's clinical condition.
检查血红蛋白水平影响白细胞增多使用高铁血红蛋白方法，导致吸光度增加，测量血红蛋白水平显著增加，假血样品用Drabkins溶液稀释，在3000转/分离心10分钟，然后用λ 546nm光度计测量上清的吸光度。本研究的目的是分析在有和没有离心的情况下，用相同的血红蛋白法检测白细胞水平的差异。这种类型的研究是观察性研究实验室。研究设计为横断面研究。选取2014年2 - 4月用血液学分析仪(CEL-DYN Ruby)检测白细胞计数大于20000 / uL的患者的剩余血液样本，根据标准分为4组，第1组。白细胞计数20000 / uL-29 999 / mL, II组。30000 / uL-39 999 / uL, III组。40000 / uL- 49999 / uL，ⅳ组50000 / uL以上。每组取20个样本，共取80个样本。分析结果显示，在样品白细胞计数中，经离心和不经离心的血红蛋白水平无显著差异，p = 0.000 < 0.05 α。白细胞浊度影响离心前后血红蛋白水平的差异，浊度越大，血红蛋白白细胞水平差异越大，本研究根据白细胞数量标准得到的血红蛋白水平检查结果为:ⅰ组0.22±0.07 g / dL，ⅱ组0.40±0.22 g / dL，ⅲ组0.40±0.22 g / dL。0.44±0.14 g / dL，第四组0.85±0.41 g / dL。样品中血红蛋白水平在高铁血红蛋白法白细胞计数超过20000 / uL时需要离心机使血红蛋白水平适当超过患者的临床情况。
{"title":"PERBEDAAN KADAR HEMOGLOBIN METODE SIANMETHEMOGLOBIN DENGAN DAN TANPA SENTRIFUGASI PADA SAMPEL LEUKOSITOSIS","authors":"Wahdah Norsiah","doi":"10.31964/MLTJ.V1I2.19","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.19","url":null,"abstract":"Examination of hemoglobin levels influenced leukocytosis sianmethemoglobin method that causes increased absorbance measurements of hemoglobin levels increased significantly and the false blood sample that has been diluted with a solution Drabkins in centrifugation at 3000 rpm for 10 minutes and then the absorbance of the supernatant was measured with a photometer at λ 546 nm. This study aimed to analyze the differences in hemoglobin level examination siamethemoglobin method with and without centrifugation at sample leukocytosis. This type of research is observational research laboratory. The study design was cross-sectional study. Samples were taken from the remaining blood samples of patients who have been examined leukositnya number more than 20,000 / uL with Hematology Analyzer (CEL-DYN Ruby) February-April 2014, and were divided into 4 groups based on criteria that group 1. leukocyte count of 20,000 / uL-29 999 / mL, group II. 30,000 / uL-39 999 / uL, the group III. 40,000 / uL-49,999 / uL, the group IV. More than 50,000 / uL. The number of samples taken were 20 samples of each group, a total sample of 80 samples. The analysis showed no significant difference in hemoglobin levels siamethemoglobin method with and without centrifugation at sample leukocytosis with a value of p = 0.000 less than 0.05 α. Leukocytosis Turbidity affects the difference in hemoglobin levels with and without centrifugation, the higher the number the greater the difference in leukocyte levels of hemoglobin, hemoglobin level examination results of the study based on the criteria of the number of leukocytes obtained by the difference in hemoglobin levels with and without centrifugation in group I. 0.22 ± 0.07 g / dL, group II 0.40 ± 0.22 g / dL, a group III. 0.44 ± 0.14 g / dL, Group IV. 0.85 ± 0.41 g / dL. The level of hemoglobin in the sample sianmethemoglobin method leukocytosis with more than 20,000 / uL need a centrifuge so that appropriate hemoglobin levels over the patient's clinical condition.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"72-83"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69806952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tofu is a food with high protein content and the moisture content reaches 85%, so that it knows can not last long. Manufacturers know still use formaldehyde as a preservative. Handling to reduce formaldehyde levels in the know are soaked in hot water and boiled in boiling water. The purpose of this study was to determine the ratio decreased levels of formaldehyde in the know are boiled and soaked in hot water out. This type of research is True Experiment with posttest study design Only Control Group Design. The population in this study is tofu containing formalin. Then examined by spectrophotometry of formaldehyde levels in most groups as a pretest sample, others are given treatment and formalin levels checked by spectrophotometry. Data were analyzed using the Mann-Whitney test. Results of this study the average levels of formaldehyde in the know before the treatment is 68.668 ppm. Decreased levels of formaldehyde in formalin know after boiling for 10 minutes was 64.77%. Decreased levels of formaldehyde in formalin know with the treatment of immersion in hot water for 10 minutes is 33.1%. Based on statistical tests that have been conducted, it was found a significant difference between the reduced levels of formaldehyde in formalin boiled out and the know formalin soaked in hot water with sig. 0,000. Boiling know formalin reduce levels of formaldehyde greater than soaking out in hot water.
{"title":"Perbandingan Penurunan Kadar Formalin pada Tahu yang Direbus dan Direndam Air Panas","authors":"Akhmad Muntaha, Haitami Haitami, Nurul Hayati","doi":"10.31964/MLTJ.V1I2.20","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I2.20","url":null,"abstract":"Tofu is a food with high protein content and the moisture content reaches 85%, so that it knows can not last long. Manufacturers know still use formaldehyde as a preservative. Handling to reduce formaldehyde levels in the know are soaked in hot water and boiled in boiling water. The purpose of this study was to determine the ratio decreased levels of formaldehyde in the know are boiled and soaked in hot water out. This type of research is True Experiment with posttest study design Only Control Group Design. The population in this study is tofu containing formalin. Then examined by spectrophotometry of formaldehyde levels in most groups as a pretest sample, others are given treatment and formalin levels checked by spectrophotometry. Data were analyzed using the Mann-Whitney test. Results of this study the average levels of formaldehyde in the know before the treatment is 68.668 ppm. Decreased levels of formaldehyde in formalin know after boiling for 10 minutes was 64.77%. Decreased levels of formaldehyde in formalin know with the treatment of immersion in hot water for 10 minutes is 33.1%. Based on statistical tests that have been conducted, it was found a significant difference between the reduced levels of formaldehyde in formalin boiled out and the know formalin soaked in hot water with sig. 0,000. Boiling know formalin reduce levels of formaldehyde greater than soaking out in hot water.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"84-90"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: Bacteremia is one public health problem. Bacteremia was ranked as the tenth leading cause of death in the United States. Gram staining of buffy coat can be used for early detection of patients with bacteremia. Rapid diagnostic tests with these Gram staining can be interpreted within an hour and the research that has been conducted Richmond et al. 2002, have a sensitivity of 75% and specificity of 79%. This study aimed to study the sensitivity and specificity of Gram buffy coat with the gold standard blood culture media biphasic fever suspected patients. This study was an observational study with cross sectional study. The study population was suspected fever patients in hospitals Ratu Zalecha Martapura months from March to May 2013. The samples were suspect fever patients in hospitals Queen Zalecha Martapura March-May 2013 were taken by purposive with the inclusion criteria: Patients District General Hospital Ratu Zalecha Martapura, aged ≥10 years, had fever ≥ 5 days, had symptoms of abdominal pain, willing to follow the study. Gram staining microscopic results were analyzed by application epicalc. Microscopic results buffy coat Gram staining Gram-negative rods are found as many as seven (26%) and were not found Gram-negative rods as many as 20 (74%). Culture results of this study are Gram negative bacterial growth as much as 6 (22%) and no growth as many as 21 (78%). Conclusion The results of microscopic Gram stain buffy coat has a value of 67% sensitivity and 86% specificity. Keywords: Gram stain, Buffy Coat, bacteraemia Abstrak: Bakteremia adalah salah satu masalah kesehatan masyarakat. Bakteremia menempati peringkat kesepuluh sebagai penyebab utama kematian di Amerika Serikat (Kung H et.al., 2008). Pewarnaan Gram dari buffy coat dapat digunakan untuk deteksi awal pasien bakteremia. Tes diagnostik yang cepat dengan pewarnaan Gram ini dapat di interpretasikan dalam waktu satu jam dan dalam penelitian yang telah dilakukan Richmond et al. 2002, mempunyai sensitifitas 75% dan spesifisitas 79%. Penelitian ini dimaksudkan untuk mempelajari sensitifitas dan spesifisitas pewarnaan Gram buffy coat dengan gold standar kultur darah media bifasik pada pasien tersangka demam. Penelitian ini merupakan penelitian observasional dengan rancangan cross sectional study. Populasi penelitian adalah pasien tersangka demam di RSUD Ratu Zalecha Martapura bulan Maret-Mei 2013. Sampel penelitian adalah pasien tersangka demam di RSUD Ratu Zalecha Martapura bulan Maret-Mei 2013 yang diambil secara purposive dengan Kriteria inklusi : Pasien Rumah Sakit Umum Daerah Ratu Zalecha Martapura, berumur ≥10 tahun, memiliki gejala demam ≥ 5 hari, memiliki gejala sakit perut, bersedia mengikuti penelitian. Hasil mikroskopis pewarnaan Gram dianalisis dengan aplikasi epicalc. Hasil mikroskopis pewarnaan Gram buffy coat yaitu ditemukan batang Gram negatif sebanyak 7 (26%) dan yang tidak ditemukan batang Gram negatif sebanyak 20 (74%). Hasil kultur penelitian ini terdapat
摘要:菌血症是一个公共卫生问题。在美国，菌血症被列为第十大死因。革兰氏染色法可用于早期发现菌血症患者。这些革兰氏染色的快速诊断测试可以在一小时内进行解释，Richmond等人2002年进行的研究灵敏度为75%，特异性为79%。本研究以双相热疑似患者的金标准血培养培养基为检测对象，探讨革兰氏白皮毛的敏感性和特异性。本研究为横断面观察性研究。研究人群为2013年3月至5月在拉图扎利查马塔普拉医院就诊的疑似发热患者。样本为2013年3月- 5月在Zalecha Martapura皇后医院就诊的疑似发热患者，入选标准为:患者年龄≥10岁，发热≥5天，有腹痛症状，愿意随访研究。革兰氏染色镜检结果应用上标分析。镜下结果革兰氏染色的革兰氏阴性杆状体多见7例(26%)，未见革兰氏阴性杆状体多见20例(74%)。本研究培养结果为革兰氏阴性菌生长6株(22%)，无生长21株(78%)。结论显微镜下革兰氏染色法检测结果敏感性为67%，特异性为86%。关键词:革兰氏染色;褐皮菌;菌血症;[j] [j] .微生物菌病(menempati peringkat kesepuluh sebagai penyebab utama kematian)。, 2008)。Pewarnaan Gram达里米色大衣dapat digunakan untuk deteksi是一种细菌血症。诊断yang cepat dengan pewarnaan Gram ini dapat di interpretasikan dalam waktu satu jam dan dalam penelitian yang telah dilakukan Richmond et al. 2002，孟山都的敏感性为75%，特异性为79%。Penelitian ini dimaksudkan untuk mempelajari sensitifitas dan speifisitas pewarnaan Gram暗色大衣dunan金标文化媒体biasik paada pasien tersangka demam。Penelitian ini merupakan Penelitian观测的横断面研究。2013年12月1日，泰国首都曼谷。2013年3月1日，《中华人民大学学报》发表了《中华人民大学学报》、《中华人民大学学报》、《中华人民大学学报》、《中华人民大学学报》、《中华人民大学学报》等文献。黄芩的显微分析及其在外皮上的应用。Hasil mikroskopis pewarnaan Gram灰褐色外套yitu ditemukan batang Gram阴性sebanyak 7 (26%) dan yang tidak ditemukan batang Gram阴性sebanyak 20(74%)。Hasil culture penelitian ini terdapat pertumbuhan bakteri Gram阴性sebanyak 6(22%)和tidak terdapat pertumbuhan sebanyak 21(78%)。kespenpenan hasil mikroskopis pewarnaan和Gram暗色被毛的敏感性为67%，特异性为86%。Kata kunci:白豆，白毛，面包菌病
{"title":"Pewarnaan Gram Buffy Coat untuk Deteksi Awal Pasien Bakteremia","authors":"Leka Lutpiatina","doi":"10.31964/MLTJ.V1I1.15","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I1.15","url":null,"abstract":"Abstract: Bacteremia is one public health problem. Bacteremia was ranked as the tenth leading cause of death in the United States. Gram staining of buffy coat can be used for early detection of patients with bacteremia. Rapid diagnostic tests with these Gram staining can be interpreted within an hour and the research that has been conducted Richmond et al. 2002, have a sensitivity of 75% and specificity of 79%. This study aimed to study the sensitivity and specificity of Gram buffy coat with the gold standard blood culture media biphasic fever suspected patients. This study was an observational study with cross sectional study. The study population was suspected fever patients in hospitals Ratu Zalecha Martapura months from March to May 2013. The samples were suspect fever patients in hospitals Queen Zalecha Martapura March-May 2013 were taken by purposive with the inclusion criteria: Patients District General Hospital Ratu Zalecha Martapura, aged ≥10 years, had fever ≥ 5 days, had symptoms of abdominal pain, willing to follow the study. Gram staining microscopic results were analyzed by application epicalc. Microscopic results buffy coat Gram staining Gram-negative rods are found as many as seven (26%) and were not found Gram-negative rods as many as 20 (74%). Culture results of this study are Gram negative bacterial growth as much as 6 (22%) and no growth as many as 21 (78%). Conclusion The results of microscopic Gram stain buffy coat has a value of 67% sensitivity and 86% specificity. \u0000Keywords: Gram stain, Buffy Coat, bacteraemia \u0000 \u0000Abstrak: Bakteremia adalah salah satu masalah kesehatan masyarakat. Bakteremia menempati peringkat kesepuluh sebagai penyebab utama kematian di Amerika Serikat (Kung H et.al., 2008). Pewarnaan Gram dari buffy coat dapat digunakan untuk deteksi awal pasien bakteremia. Tes diagnostik yang cepat dengan pewarnaan Gram ini dapat di interpretasikan dalam waktu satu jam dan dalam penelitian yang telah dilakukan Richmond et al. 2002, mempunyai sensitifitas 75% dan spesifisitas 79%. Penelitian ini dimaksudkan untuk mempelajari sensitifitas dan spesifisitas pewarnaan Gram buffy coat dengan gold standar kultur darah media bifasik pada pasien tersangka demam. Penelitian ini merupakan penelitian observasional dengan rancangan cross sectional study. Populasi penelitian adalah pasien tersangka demam di RSUD Ratu Zalecha Martapura bulan Maret-Mei 2013. Sampel penelitian adalah pasien tersangka demam di RSUD Ratu Zalecha Martapura bulan Maret-Mei 2013 yang diambil secara purposive dengan Kriteria inklusi : Pasien Rumah Sakit Umum Daerah Ratu Zalecha Martapura, berumur ≥10 tahun, memiliki gejala demam ≥ 5 hari, memiliki gejala sakit perut, bersedia mengikuti penelitian. Hasil mikroskopis pewarnaan Gram dianalisis dengan aplikasi epicalc. Hasil mikroskopis pewarnaan Gram buffy coat yaitu ditemukan batang Gram negatif sebanyak 7 (26%) dan yang tidak ditemukan batang Gram negatif sebanyak 20 (74%). Hasil kultur penelitian ini terdapat","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"38-46"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ratih Dewi Dwiyanti, Nurlailah Nurlailah, Indah Kurnia Widiningsih
Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi. Keywords: Water decoction of leaves Binahong, Salmonella typhi, antibacterial. Abstrak: Penyakit tifus atau dikenal dengan demam tifoid atau demam enterik adalah salah satu penyakit demam akut yang disebabkan oleh bakteri Salmonella typhi. Pengobatan demam tifoid biasanya menggunakan antibiotik, penggunaan antibiotik dapat menimbulkan efek samping. Masyarakat saat ini banyak menggunakan pengobatan dengan bahan alami, salah satunya adalah Binahong (Anredera cordifolia) yang mengandung senyawa Alkaloid, Polifenol, Flavonoid, Saponin, dan Antrakuinon yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui efektifitas air rebusan daun Binahong terhadap pertumbuhan Salmonella typhi secara in vitro. Jenis penelitian ini adalah true eksperiment dengan rancangan penelitian Posttest Only Control Group Design dan metode yang digunakan adalah difusi (sumuran) dengan 5 perlakuan. Konsentrasi air rebusan daun Binahong yang dgunakan adalah 20%, 40%, 60%, 80% dan 100%. Hasil penelitian didapatkan zona hambat air rebusan daun Binahong terhadap Salmonella typhi pada konsentrasi 20%, 40%, 60%, 80% adalah 0 mm, sedangkan pada konsentrasi 100% didapatkan zona hambat sebesar 11 mm. Kesimpulan penelitian ini adalah air rebusan daun Binahong pada konsentrasi 100% memiliki kemampuan menghambat pertumbuhan Salmonella typhi, namun hasil ini belum efektif karena masih dalam kategori resisten. Disarankan bagi peneliti selanjutnya untuk meningkatkan konsentrasi air rebusan daun binahong atau menggunakan ektrak alkohol daun binahong untuk
斑疹伤寒是由伤寒沙门氏菌引起的一种急性发热性疾病。治疗伤寒通常使用抗生素，使用抗生素会引起副作用。如今，人们使用天然成分进行治疗，其中一种是含有生物碱、多酚、类黄酮、皂素和蒽醌的天然成分。本研究旨在确定滨拿红叶水煎液对伤寒沙门菌体外生长的抑制作用。这种类型的研究是真正的实验后测研究设计，只有对照组设计，使用的方法是扩散(井)5处理。烹煮水的浓度分别为20%、40%、60%、80%和100%。结果表明:滨拿红叶水煎液在浓度为20%、40%、60%、80%时对伤寒沙门菌的抑菌带为0 mm，在浓度为100%时抑菌带为11 mm。由此得出，滨拿红叶水煎液浓度为100%时具有抑制伤寒沙门菌生长的能力，但由于仍处于耐药范畴，这些结果尚未取得效果。建议进一步研究提高红花叶水煎液浓度或用红花叶醇提物抑制伤寒沙门菌的生长。关键词:滨拿红叶水煎液;伤寒沙门氏菌;抗菌;摘要:伤寒沙门菌(Salmonella typhi);伤寒沙门菌(Salmonella typhi)。彭巴丹类抗生素biasanya孟古那坎抗生素，彭古那坎抗生素dapat menbulkan efek采样。黄酮类生物碱、多酚、黄酮类、皂苷类、黄酮类抗细菌活性物质。Penelitian ini bertujuan untuk mengetahui fektifitas air rebusan, Binahong, pertumbuhan伤寒沙门氏菌的体外培养。Jenis penelitian ini adalah真实实验dengan ranancan penelian后验对照组设计dan metod yang digunakan adalah difusi (sumuran) dengan 5 perlakuan。康森特拉斯的空气比纳洪的空气比纳洪的空气比纳洪的空气比纳洪的空气比纳洪的空气比纳洪的空气比纳洪高20%、40%、60%、80%、100%。Hasil penelitian didapatkan zona hambat air rebusan, Binahong terhadap伤寒沙门菌20%，40%，60%，80% adalah 0 mm, sedangkan pada konsentrasi 100% didapatkan zona hambat sebesar 11 mm。沙门菌伤寒，耐药沙门菌。【中文译文】:Disarankan bagi peneliti selanjutnya untuk meningkatkan konsentrasi空气rebusan duan binahong atau mongunakan ektrak酒精duan binahong untuk menghambat pertumbuhan伤寒沙门氏菌。Kata kunci:空气污染，伤寒沙门氏菌，抗细菌。
{"title":"Efektivitas Air Rebusan Daun Binahong (Anredera cordifolia) Terhadap Pertumbuhan Salmonella typhi","authors":"Ratih Dewi Dwiyanti, Nurlailah Nurlailah, Indah Kurnia Widiningsih","doi":"10.31964/MLTJ.V1I1.7","DOIUrl":"https://doi.org/10.31964/MLTJ.V1I1.7","url":null,"abstract":"Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi. \u0000Keywords: Water decoction of leaves Binahong, Salmonella typhi, antibacterial. \u0000 \u0000Abstrak: Penyakit tifus atau dikenal dengan demam tifoid atau demam enterik adalah salah satu penyakit demam akut yang disebabkan oleh bakteri Salmonella typhi. Pengobatan demam tifoid biasanya menggunakan antibiotik, penggunaan antibiotik dapat menimbulkan efek samping. Masyarakat saat ini banyak menggunakan pengobatan dengan bahan alami, salah satunya adalah Binahong (Anredera cordifolia) yang mengandung senyawa Alkaloid, Polifenol, Flavonoid, Saponin, dan Antrakuinon yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui efektifitas air rebusan daun Binahong terhadap pertumbuhan Salmonella typhi secara in vitro. Jenis penelitian ini adalah true eksperiment dengan rancangan penelitian Posttest Only Control Group Design dan metode yang digunakan adalah difusi (sumuran) dengan 5 perlakuan. Konsentrasi air rebusan daun Binahong yang dgunakan adalah 20%, 40%, 60%, 80% dan 100%. Hasil penelitian didapatkan zona hambat air rebusan daun Binahong terhadap Salmonella typhi pada konsentrasi 20%, 40%, 60%, 80% adalah 0 mm, sedangkan pada konsentrasi 100% didapatkan zona hambat sebesar 11 mm. Kesimpulan penelitian ini adalah air rebusan daun Binahong pada konsentrasi 100% memiliki kemampuan menghambat pertumbuhan Salmonella typhi, namun hasil ini belum efektif karena masih dalam kategori resisten. Disarankan bagi peneliti selanjutnya untuk meningkatkan konsentrasi air rebusan daun binahong atau menggunakan ektrak alkohol daun binahong untuk ","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"1 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69806685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: Research on the content of iodine in salt Kitchen in the Market Martapura March 2014 aims to determine the iodine content in the salt that is sold in the market Batuah Martapura and look for compliance with quality standards ISO 01-3556.2-1994 / Rev 2000. The method used in this study is a descriptive survey. The sample used in this study is a salt-branded and not branded, with a total sampling technique sampling. The research found 67% salt containing KIO3, and 33% of salt that does not contain KIO3, KIO3 in salt levels ranged from 8.68 ppm - 79.94 ppm, 53% salt that does not qualify SNI and only 47% were eligible, 100% salt not branded ineligible SNI and 42% SALT branded ineligible SNI. The public to be cautious in buying salt consumption for everyday purposes, and to the relevant agencies to conduct outreach and firm action against violations that exist in society against the marketing of salt that does not comply with permitted. Keywords: salt branded and not branded, Kadar KIO3 Abstrak: Penelitian tentang Kandungan Iodium pada Garam Dapur di Pasar Martapura Maret 2014 bertujuan untuk menentukan kandungan iodium yang ada pada garam dapur yang dijual di Pasar Batuah Martapura serta melihat kesesuaiannya dengan baku mutu SNI 01-3556.2-1994/Rev 2000. Metode yang digunakan dalam penelitian ini adalah Survey Deskriptif. Sampel yang digunakan dalam penelitian adalah garam bermerk dan tidak bermerk, dengan teknik pengambilan sampel secara total sampling. Hasil penelitian ditemukan 67% garam yang mengandung KIO3, dan 33% garam yang tidak mengandung KIO3, Kadar KIO3 dalam garam berkisar antara 8.68 ppm – 79.94 ppm, 53% garam yang tidak memenuhi syarat SNI dan hanya 47% yang memenuhi syarat, 100% garam tidak bermerk tidak memenuhi syarat SNI dan 42% GARAM bermerk tidak memenuhi syarat SNI. Masyarakat agar berhati-hati dalam membeli garam konsumsi untuk keperluan sehari-hari, dan kepada instansi terkait agar melakukan penyuluhan dan tindakan yang tegas terhadap pelanggaran yang ada di masyarakat terhadap pemasaran garam yang tidak sesuai dengan yang diizinkan. Kata kunci: Garam bermerk dan tidak bermerk, Kadar KIO3
摘要:Martapura市场食盐厨房碘含量研究2014年3月旨在检测Batuah Martapura市场销售的食盐中碘含量，并检查是否符合ISO 01-3556.2-1994 / Rev 2000的质量标准。本研究使用的方法是描述性调查。本研究使用的样本为盐烙印和非烙印，采用全采样技术采样。研究发现，67%的盐含有KIO3, 33%的盐不含KIO3，盐的KIO3含量在8.68 ppm - 79.94 ppm之间，53%的盐不符合SNI标准，只有47%的盐符合SNI标准，100%的盐没有被标记为不符合SNI标准，42%的盐被标记为不符合SNI标准。公众在购买日常食用食盐时要谨慎，并要求相关机构开展外展和坚决行动，打击社会上存在的违法行为，反对销售不符合规定的食盐。摘要:Penelitian tentang Kandungan Iodium paada Garam Dapur di Pasar Martapura市场2014 bertujuan untuk menentukan Kandungan Iodium yang ada padada Garam Dapur yang dijual di Pasar Batuah Martapura serta melihat kesessuaiannya dengan baku mutu SNI 01-3556.2-1994/Rev 2000。Metode yang digunakan dalam penelitian ini adalah调查报告。Sampel yang digunakan dalam penelitian adalah garam bermerk dan tidak bermerk, dengan teknik pengambilan Sampel secara total sampling。Hasil penelitian ditemukan 67% garam yang mengandung KIO3, dan 33% garam yang tidak mengandung KIO3, Kadar KIO3 dalam garam berkisar antara 8.68 ppm - 79.94 ppm, 53% garam yang tidak memenhi syarat SNI, 53% garam yang tidak memenhi syarit SNI, 100% garam tidak bermerk memenhi syarit SNI dan 42% garam bermerk memenhi syarit SNI。Masyarakat agar berhati-hati dalam membeli garam konsumsi untuk keperluan sehari-hari, dan kepada instanterkait agar melakukan penyuluhan dan tindakan yang tegas terhadap pelanggaran yang ada di Masyarakat terhadap pemasaran garam yang tidak sesuai dengan yang diizinkan。Kata kunci: Garam bermerk dan tidak bermerk, Kadar KIO3
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