The principle of organic substances determination in water samples is oxidized by an excess amount of KMnO4, then it is reduced excess amount of oxalic acid, the excess of oxalic acid is titrated back with KMnO4. Based on the procedures in ISO 06-6989.22-2004, a length of boiling time for organic substances determination in water samples is for 10 minutes. This study aimed to determine the effect of boiling time length to the accuracy of the organic substances determination result in water samples. This study was true experiment study with comparative study design. Samples were organic substances, specifically oxalic acid in distilled water in a concentration of 54 mg/L, which was examined for the organic substances level with a boiling time of 5, 10 and 15 minutes. The result on each boiling time length consecutively were 52,8 mg/L; 54,5 mg/L; dan 55,1 mg/L. The conclusion of the study was there was effect of boiling time length to the accuracy of organic substances determination result in water samples with the significance value of 0.002 (<0,05). It is recommended to continue the study using a direct sample from a field with the addition of sample matrix.
{"title":"Ketepatan Hasil dan Variasi Waktu Pendidihan Pemeriksaan Zat Organik","authors":"Haitami Haitami, Dinna Rakhmina, Syahid Fakhridani","doi":"10.31964/mltj.v2i2.117","DOIUrl":"https://doi.org/10.31964/mltj.v2i2.117","url":null,"abstract":"The principle of organic substances determination in water samples is oxidized by an excess amount of KMnO4, then it is reduced excess amount of oxalic acid, the excess of oxalic acid is titrated back with KMnO4. Based on the procedures in ISO 06-6989.22-2004, a length of boiling time for organic substances determination in water samples is for 10 minutes. This study aimed to determine the effect of boiling time length to the accuracy of the organic substances determination result in water samples. This study was true experiment study with comparative study design. Samples were organic substances, specifically oxalic acid in distilled water in a concentration of 54 mg/L, which was examined for the organic substances level with a boiling time of 5, 10 and 15 minutes. The result on each boiling time length consecutively were 52,8 mg/L; 54,5 mg/L; dan 55,1 mg/L. The conclusion of the study was there was effect of boiling time length to the accuracy of organic substances determination result in water samples with the significance value of 0.002 (<0,05). It is recommended to continue the study using a direct sample from a field with the addition of sample matrix.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"69 1","pages":"61-65"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cassava is the third staple food often used by people to make a variety of products. Carbohydrates from cassava also contains toxic compound such as cyanide which can inhibit the action of respiratory enzymes. Cyanide in cassava can be removed by boiling or frying process. The aim of research was to find out the cyanide level differences between boiled cassava and fried cassava. This type of research was experiment, with design of comparative studies approach. Cyanide levels were examined in 10 samples with 2 treatments using Argentometry Volhard titration method. The result showed the average of cyanide levels in boiled cassava was 0.772 ppm and in fried cassava was 1.069 ppm. The percentage of cyanide levels reduction in boiled cassava was 28,78%, while for the fried cassava was 0%. The statistical test obtained the value of p <0.05, that showed there was significant difference of cyanide level between boiled cassava and fried cassava. Based on these results, it is advisable to choose the boiling process for cassava consumption. Next research can be conducted on comparative levels of cyanide in the cassava root and cassava leaves.
{"title":"Kadar Sianida Singkong Rebus dan Singkong Goreng","authors":"Yeni Purwati, Anny Thuraidah, Dinna Rakhmina","doi":"10.31964/mltj.v2i2.93","DOIUrl":"https://doi.org/10.31964/mltj.v2i2.93","url":null,"abstract":"Cassava is the third staple food often used by people to make a variety of products. Carbohydrates from cassava also contains toxic compound such as cyanide which can inhibit the action of respiratory enzymes. Cyanide in cassava can be removed by boiling or frying process. The aim of research was to find out the cyanide level differences between boiled cassava and fried cassava. This type of research was experiment, with design of comparative studies approach. Cyanide levels were examined in 10 samples with 2 treatments using Argentometry Volhard titration method. The result showed the average of cyanide levels in boiled cassava was 0.772 ppm and in fried cassava was 1.069 ppm. The percentage of cyanide levels reduction in boiled cassava was 28,78%, while for the fried cassava was 0%. The statistical test obtained the value of p <0.05, that showed there was significant difference of cyanide level between boiled cassava and fried cassava. Based on these results, it is advisable to choose the boiling process for cassava consumption. Next research can be conducted on comparative levels of cyanide in the cassava root and cassava leaves.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"46-50"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Candida albicans infection is the cause of candidiasis. Candidiasis treatment can be done with a variety of antifungal drugs, one of them is rhizome of kencur (Kaempferia galanga L.). The Rhizome of kencur is selected as a traditional medicine because it contains chemical compounds such as flavonoids, tannins, saponins and essential oil that serves as an antifungal. This study aimed to determine the minimal inhibitory and minimal killing power and also an influence of kencur rhizome extract on the growth of Candida albicans in vitro. This research was true experimental design with posttest only control group design with tube dilution method. Results of Minimal Inhibitory Concentrations (MICs) research showed there was no clarity at concentration of 20 mg/mL, 30 mg/mL, 40 mg/mL, and it shows clarity at concentration of 50 mg/mL and 60 mg/mL. Results of Minimum Bactericidal Concentrations (MBCs) showed the number of colonies at concentration of 20 mg/mL were 84 colonies, concentration of 30 mg/mL were 48 colonies, concentration of 40 mg/mL were 27 colonies, concentration of mg/mL were 12 colonies and concentration of 60 mg/mL were 0 colony. Based on linear regression test, the result showed significance value of 0.000 <ɑ = 0.05 so it can be concluded that there is a kencur rhizome extract influence on the growth of Candida albicans in vitro with minimal inhibitory concentrations is the concentration of 50 mg/mL and the minimal bactericidal concentrations 60 mg/mL. Further studies are required regarding kencur rhizome extract (Kaempferia galanga L.) in inhibiting and bactericidal microorganisms other than Candida albicans.
{"title":"Potensi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Menghambat Pertumbuhan Candida albicans","authors":"Annisa Rahmi, Erpan Roebiakto, Leka Lutpiatina","doi":"10.31964/mltj.v2i2.94","DOIUrl":"https://doi.org/10.31964/mltj.v2i2.94","url":null,"abstract":"Candida albicans infection is the cause of candidiasis. Candidiasis treatment can be done with a variety of antifungal drugs, one of them is rhizome of kencur (Kaempferia galanga L.). The Rhizome of kencur is selected as a traditional medicine because it contains chemical compounds such as flavonoids, tannins, saponins and essential oil that serves as an antifungal. This study aimed to determine the minimal inhibitory and minimal killing power and also an influence of kencur rhizome extract on the growth of Candida albicans in vitro. This research was true experimental design with posttest only control group design with tube dilution method. Results of Minimal Inhibitory Concentrations (MICs) research showed there was no clarity at concentration of 20 mg/mL, 30 mg/mL, 40 mg/mL, and it shows clarity at concentration of 50 mg/mL and 60 mg/mL. Results of Minimum Bactericidal Concentrations (MBCs) showed the number of colonies at concentration of 20 mg/mL were 84 colonies, concentration of 30 mg/mL were 48 colonies, concentration of 40 mg/mL were 27 colonies, concentration of mg/mL were 12 colonies and concentration of 60 mg/mL were 0 colony. Based on linear regression test, the result showed significance value of 0.000 <ɑ = 0.05 so it can be concluded that there is a kencur rhizome extract influence on the growth of Candida albicans in vitro with minimal inhibitory concentrations is the concentration of 50 mg/mL and the minimal bactericidal concentrations 60 mg/mL. Further studies are required regarding kencur rhizome extract (Kaempferia galanga L.) in inhibiting and bactericidal microorganisms other than Candida albicans.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"70-76"},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract : Interleukin-10 as pro-inflammatory cytokines have a role to protect the damage of severe malaria. Kelakai contain bioactive compounds that have anti-inflammatory activity. The purpose of this study was to determine the potential of the extract kelakai on levels of IL-10 in mice BALB / s infected by P. berghei ANKA. This study is true experimental studies with posttest-only with Control Group Design. The treatment group was divided into 8 groups. Two groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW. Four groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW 3 hours after infection and when the parasitaemia reaches 15-20%. Negative controls do not receive the extract kelakai and parasitic infections. Positive controls get a parasitic infection. Treatment was given for 4 days. Blood samples were taken 24 hours after the last treatment. Levels of IL-10 were measured by sandwich ELISA method. Data were analyzed with Games Howell test, with a confidence level of 95%. There were no significant differences between treatment groups. Extract kelakai Giving oral dose of 10 mg/KgBW and 100 mg/KgBW not significantly increase the levels of interleukin-10. Keywords: Stenochlaena palustris (Burm.f) bedd; Interleukin-10 Abstrak : Interleukin-10 sebagai Sitokin pro-inflamasi memiliki peran melindungi kerusakan pada malaria berat. Kelakai mengandung senyawa bioaktif yang mempunyai aktifitas anti-inflamasi. Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak kelakai terhadap kadar IL-10 pada mencit BALB/s yang diinfeksi P. berghei ANKA. Penelitian ini merupakan studi eksperimental murni dengan Posttest-only with Control Group Design. Kelompok perlakuan dibagi menjadi 8 kelompok. Dua kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan. Empat kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan 3 jam setelah infeksi dan pada saat parasitemia mencapai 15-20%. Kontrol negatif tidak mendapat ekstrak kelakai dan infeksi parasit. Kontrol positif mendapat infeksi parasit. Perlakuan diberikan selama 4 hari. Sampel darah diambil 24 jam setelah perlakuan terakhir. Kadar Il-10 diukur dengan ELISA metode sandwich. Data dianalisa dengan tes Games Howell, dengan tingkat kepercayaan 95%. Tidak didapatkan perbedaan bermakna antar kelompok perlakuan. Pemberian Ekstrak kelakai secara oral dosis 10 mg/Kg berat badan dan 100 mg/Kg berat badan tidak signifikan meningkatkan kadar Interleukin-10. Kata-kata Kunci : Stenochlaena palustris (Burm.f) Bedd; Interleukin-10
摘要/ Abstract摘要:白细胞介素-10作为促炎细胞因子在重症疟疾损伤中具有保护作用。克拉凯含有具有抗炎活性的生物活性化合物。本研究的目的是确定克拉凯提取物对伯氏单胞杆菌感染小鼠BALB / s白细胞介素-10水平的影响。本研究为纯后测实验研究，采用对照组设计。治疗组分为8组。两组患者分别口服克拉凯提取物10 mg/kgBW和100 mg/kgBW。4组小鼠在感染后3 h及寄生虫率达到15-20%时，分别口服克拉凯提取物10 mg/kgBW和100 mg/kgBW。阴性对照组不接受克拉凯提取物和寄生虫感染。阳性对照组感染寄生虫。治疗4天。最后一次治疗后24小时采集血样。采用夹心ELISA法检测IL-10水平。数据分析采用Games Howell检验，置信水平为95%。治疗组间无显著差异。口服剂量10 mg/KgBW和100 mg/KgBW均未显著提高白细胞介素-10水平。毕业论文关键词:古窄螯虾;摘要:白细胞介素-10 (Interleukin-10)具有促炎作用。克拉凯孟加东森雅瓦生物制剂阳孟加东抗炎药。Tujuan penelitian ini adalah untuk mengetahui potential, ekstrak kelakai terhadap kadar IL-10 pada menencit BALB/s, yang diinfeksi P. berghei ANKA。采用对照组设计，对实验小鼠进行后测。Kelompok perlakuan dibagi menjadi 8 Kelompok。杜阿克龙柏，每口10毫克/公斤巴丹丹和100毫克/公斤巴丹丹。乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木。控制革拉克氏菌和革拉克氏菌阴性。控制白蛉阳性。Perlakuan diberikan selama 4 hari。Sampel darah diambil 24 jam setelah perlakuan terakhir。卡达尔Il-10酶联免疫吸附测定方法。数据分析显示，在游戏中，游戏的成功率为95%。Tidak didapatkan perbedaan bermakna antar kelompok perlakuan。Pemberian Ekstrak kelakai secara口服剂量10 mg/Kg berat badan和100 mg/Kg berat badan显着性脑膜炎katkan kadar白介素-10。Kata-kata Kunci: Stenochlaena palustris(缅甸)Bedd;白细胞介素- 10”
{"title":"PENGARUH EKSTRAK KELAKAI (Stenochlaena palustris (Burm.f) Bedd) TERHADAP KADAR INTERLEUKIN-10 (IL-10) MENCIT","authors":"Denny P.N.H. Margono, E. Suhartono, H. Arwati","doi":"10.31964/MLTJ.V2I1.43","DOIUrl":"https://doi.org/10.31964/MLTJ.V2I1.43","url":null,"abstract":"Abstract : Interleukin-10 as pro-inflammatory cytokines have a role to protect the damage of severe malaria. Kelakai contain bioactive compounds that have anti-inflammatory activity. The purpose of this study was to determine the potential of the extract kelakai on levels of IL-10 in mice BALB / s infected by P. berghei ANKA. This study is true experimental studies with posttest-only with Control Group Design. The treatment group was divided into 8 groups. Two groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW. Four groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW 3 hours after infection and when the parasitaemia reaches 15-20%. Negative controls do not receive the extract kelakai and parasitic infections. Positive controls get a parasitic infection. Treatment was given for 4 days. Blood samples were taken 24 hours after the last treatment. Levels of IL-10 were measured by sandwich ELISA method. Data were analyzed with Games Howell test, with a confidence level of 95%. There were no significant differences between treatment groups. Extract kelakai Giving oral dose of 10 mg/KgBW and 100 mg/KgBW not significantly increase the levels of interleukin-10. \u0000Keywords: Stenochlaena palustris (Burm.f) bedd; Interleukin-10 \u0000 \u0000 \u0000Abstrak : Interleukin-10 sebagai Sitokin pro-inflamasi memiliki peran melindungi kerusakan pada malaria berat. Kelakai mengandung senyawa bioaktif yang mempunyai aktifitas anti-inflamasi. Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak kelakai terhadap kadar IL-10 pada mencit BALB/s yang diinfeksi P. berghei ANKA. Penelitian ini merupakan studi eksperimental murni dengan Posttest-only with Control Group Design. Kelompok perlakuan dibagi menjadi 8 kelompok. Dua kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan. Empat kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan 3 jam setelah infeksi dan pada saat parasitemia mencapai 15-20%. Kontrol negatif tidak mendapat ekstrak kelakai dan infeksi parasit. Kontrol positif mendapat infeksi parasit. Perlakuan diberikan selama 4 hari. Sampel darah diambil 24 jam setelah perlakuan terakhir. Kadar Il-10 diukur dengan ELISA metode sandwich. Data dianalisa dengan tes Games Howell, dengan tingkat kepercayaan 95%. Tidak didapatkan perbedaan bermakna antar kelompok perlakuan. Pemberian Ekstrak kelakai secara oral dosis 10 mg/Kg berat badan dan 100 mg/Kg berat badan tidak signifikan meningkatkan kadar Interleukin-10. \u0000Kata-kata Kunci : Stenochlaena palustris (Burm.f) Bedd; Interleukin-10","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2016-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: The objective of this research is to analyze the effects of ethanol extract of black cumin seeds (Nigella Sativa) to the expression of ET-1 Aorta given to the preeclampsia model mouse. The research design utilizes experimental and the kind of design used is posttest only control group design. Mice preeclampsia model. This research is divided into 6 groups, namely: Negative control, Positive control, Model + extract of black cumin seeds 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day. After conducting the surgery to the mouse, an examination is done to the expression of ET-1 Aorta by using Immunohistochemistry method. one way anova Were used as statistical analysis. There was an effect of treatment of giving the ethanol extract black cumin seeds of ET-1 Aorta to the preeclampsia model mouse (p<0,05).these doses of 500 mg, 1000mg, 1500mg, and 2000mg, ethanol extract of black cumin seeds has ability to decrease the ET-1 Aorta in preeclampsia model mouse. dose of 1500mg/KgBB/day is the most optimum dosage to reduce when compared to another dosages. Ethanol extract of black cumin seeds (Nigella Sativa) can decrease the expression of ET-1 Aorta in preeclampsia model mouse, particulary at dose 2000mg/Kg/BB/day sequentially Keyword : Preeclampsia; black cumin seeds (Nigella Sativa); ET-1; Aorta . Abstrak: Penelitian ini bertujuan untuk menganalisa pengaruh pemberian ekstrak etanol biji jinten hitam(Nigella Sativa) terhadap ekspresi ET-1 Aorta pada mencit model preeclampsia. Desain Penelitian ini menggunakan eksprimental (experimental) dengan jenis rancangan posttest only control group design. Menggunakan mencit model preeklampsia. Penelitian ini dibagi menjadi 6 kelompok yaitu Kontrol negatif, kontrol positif(Model), Model+ekstrak biji jinten hitam 500 mg/KgBB/hari, 1000 mg/KgBB/hari, 1500 mg/KgBB/hari, dan 2000 mg/KgBB/hari, setelah dilakukan pembedahan pada mencit kemudian dilakukan pemeriksaan ekspresi ET-1 Aorta dengan metode Immunohistokimia. Data hasil pengamatan dianalisis dengan uji one way anova. Ada pengaruh pemberian ekstrak etanol biji jinten hitam terhadap ekspresi ET-1 Aorta pada mencit model preeklampsia (p<0,05). Dosis 500mg, 1000mg, 1500mg, dan 2000mg jinten hitam mempunyai kemampuan terhadap penurunan ekspresi ET-1 Aorta Dosis yang paling optimum menurunkan adalah dosis 2000mg/KgBB/hari dibanding dosis yang lain. Ekstrak etanol biji jinten hitam (Nigella Sativa) dapat menurunkan ekspresi ET-1 Aorta pada mencit model preeklampsia terutama pada dosis 2000mg/KgBB/hari. Kata Kunci: Preeklampsia; Biji jinten hitam (Nigella sativa); ET-1; Aorta
摘要:本研究旨在分析黑孜然种子乙醇提取物对子痫前期模型小鼠ET-1主动脉表达的影响。研究设计采用实验设计，采用后测纯对照组设计。小鼠子痫前期模型。本研究分为6组，分别为:阴性对照组、阳性对照组、模型+黑孜然籽提取物500 mg/KgBB/day、1000 mg/KgBB/day、1500 mg/KgBB/day、2000 mg/KgBB/day。小鼠手术后，采用免疫组化法检测ET-1主动脉表达。采用单因素方差分析进行统计分析。ET-1主动脉黑孜然种子乙醇提取物对子痫前期模型小鼠有显著影响(p< 0.05)。500mg、1000mg、1500mg、2000mg黑孜然乙醇提取物对子痫前期模型小鼠ET-1主动脉有明显的抑制作用。与其他剂量相比，1500mg/KgBB/天的剂量是减少的最佳剂量。黑孜然种子乙醇提取物可降低子痫前期模型小鼠ET-1主动脉的表达，且连续剂量为2000mg/Kg/BB/d时效果更好。关键词:子痫前期;黑孜然种子(Nigella Sativa);ET-1;主动脉。摘要:Penelitian ini bertujuan untuk menganalisa pengarian ekstrak etanol biji jinten hitam(Nigella Sativa) terhadap ekpresi ET-1主动脉缺血模型子痫前期。实验组设计，实验组设计，实验组设计，实验组设计。孟古那坎孟克特模型子痫前期。对照阴性，对照阳性(模型)，模型+ biji jintenhitam 500 mg/KgBB/hari, 1000 mg/KgBB/hari, 1500 mg/KgBB/hari, dan 2000 mg/KgBB/hari, setelah dilakukan ppendahan ppadmenciit kemudian dilakukan pemeriksaan ekspresi ET-1主动脉内皮细胞免疫组化。数据采用单因素方差分析。ET-1对大鼠先兆子痫模型的影响(p< 0.05)。1000毫克剂量500毫克,1500毫克,丹2000毫克jinten hitam mempunyai kemampuan terhadap penurunan ekspresi ET-1主动脉剂量杨木栅最佳menurunkan adalah剂量2000毫克/ KgBB哈里dibanding剂量杨躺。estrak乙醇biji jinten hitam (Nigella Sativa) dapat menurunkan ekspresi ET-1主动脉乳头状瘤模型terutama乳头状瘤前期子痫剂量2000mg/KgBB/hari。Kata Kunci:先兆子痫;黑鬼(Nigella sativa);ET-1;主动脉
{"title":"PENGARUH EKSTRAK ETANOL BIJI JINTEN HITAM (Nigella Sativa) TERHADAP EKSPRESI ET-1 AORTA PADA MENCIT MODEL PREEKLAMPSIA","authors":"Rubiati Hipni","doi":"10.31964/MLTJ.V2I1.37","DOIUrl":"https://doi.org/10.31964/MLTJ.V2I1.37","url":null,"abstract":"Abstract: The objective of this research is to analyze the effects of ethanol extract of black cumin seeds (Nigella Sativa) to the expression of ET-1 Aorta given to the preeclampsia model mouse. The research design utilizes experimental and the kind of design used is posttest only control group design. Mice preeclampsia model. This research is divided into 6 groups, namely: Negative control, Positive control, Model + extract of black cumin seeds 500 mg/KgBB/day, 1000 mg/KgBB/day, 1500 mg/KgBB/day, and 2000 mg/KgBB/day. After conducting the surgery to the mouse, an examination is done to the expression of ET-1 Aorta by using Immunohistochemistry method. one way anova Were used as statistical analysis. There was an effect of treatment of giving the ethanol extract black cumin seeds of ET-1 Aorta to the preeclampsia model mouse (p<0,05).these doses of 500 mg, 1000mg, 1500mg, and 2000mg, ethanol extract of black cumin seeds has ability to decrease the ET-1 Aorta in preeclampsia model mouse. dose of 1500mg/KgBB/day is the most optimum dosage to reduce when compared to another dosages. Ethanol extract of black cumin seeds (Nigella Sativa) can decrease the expression of ET-1 Aorta in preeclampsia model mouse, particulary at dose 2000mg/Kg/BB/day sequentially \u0000Keyword : Preeclampsia; black cumin seeds (Nigella Sativa); ET-1; Aorta . \u0000 \u0000Abstrak: Penelitian ini bertujuan untuk menganalisa pengaruh pemberian ekstrak etanol biji jinten hitam(Nigella Sativa) terhadap ekspresi ET-1 Aorta pada mencit model preeclampsia. Desain Penelitian ini menggunakan eksprimental (experimental) dengan jenis rancangan posttest only control group design. Menggunakan mencit model preeklampsia. Penelitian ini dibagi menjadi 6 kelompok yaitu Kontrol negatif, kontrol positif(Model), Model+ekstrak biji jinten hitam 500 mg/KgBB/hari, 1000 mg/KgBB/hari, 1500 mg/KgBB/hari, dan 2000 mg/KgBB/hari, setelah dilakukan pembedahan pada mencit kemudian dilakukan pemeriksaan ekspresi ET-1 Aorta dengan metode Immunohistokimia. Data hasil pengamatan dianalisis dengan uji one way anova. Ada pengaruh pemberian ekstrak etanol biji jinten hitam terhadap ekspresi ET-1 Aorta pada mencit model preeklampsia (p<0,05). Dosis 500mg, 1000mg, 1500mg, dan 2000mg jinten hitam mempunyai kemampuan terhadap penurunan ekspresi ET-1 Aorta Dosis yang paling optimum menurunkan adalah dosis 2000mg/KgBB/hari dibanding dosis yang lain. Ekstrak etanol biji jinten hitam (Nigella Sativa) dapat menurunkan ekspresi ET-1 Aorta pada mencit model preeklampsia terutama pada dosis 2000mg/KgBB/hari. \u0000Kata Kunci: Preeklampsia; Biji jinten hitam (Nigella sativa); ET-1; Aorta","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"37-41"},"PeriodicalIF":0.0,"publicationDate":"2016-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: The levels of Biological Oxygen Demand (BOD) in the waste can be reduced by utilizing water plants such as velvetleaf (Limnocharis flava) as phytoremediation. This study aims to determine the influence of the many clumps Limnocharis flava to decreased levels of effluent BOD rubber industry with a variety clump Limnocharis flava much as 1, 2, 3, 4, and 5 clumps and clumps Limnocharis flava know the number of the most influential in lowering levels of BOD. This type of research is true experimental design with one group pretest-posttest. The research material was Limnocharis flava. The result showed the levels of initial BOD rubber waste amounted to 299.5 mg / l, decreased levels of BOD of waste rubber after being treated with Limnocharis flava with a variety of clumps. Decreased levels of BOD rubber waste for each successive treatment ranging from 1, 2, 3, 4, and 5 clumps genjer ie 13.65%, 14, 52%, 15.6%, 20.94%, and 29.05%. Based on test results obtained linear regression for each treatment their effect on levels of BOD using genjer rubber waste. Treatment using Limnocharis flava much as 5 clump results BOD levels decrease the total of up to 29.05%. This value has not met the standard the maximum allowable levels of BOD. Need soaking clumps Limnocharis flava more stout and longer so that the rubber waste reduction in BOD levels in accordance with the quality standards required.
{"title":"Pengaruh Genjer (Limnocharis flava) terhadap Penurunan Biological Oxygen Demand (BOD) Limbah Industri Karet","authors":"Anny Thuraidah, Erie Indra Puspita, Neny Oktiyani","doi":"10.31964/MLTJ.V2I1.28","DOIUrl":"https://doi.org/10.31964/MLTJ.V2I1.28","url":null,"abstract":"Abstract: The levels of Biological Oxygen Demand (BOD) in the waste can be reduced by utilizing water plants such as velvetleaf (Limnocharis flava) as phytoremediation. This study aims to determine the influence of the many clumps Limnocharis flava to decreased levels of effluent BOD rubber industry with a variety clump Limnocharis flava much as 1, 2, 3, 4, and 5 clumps and clumps Limnocharis flava know the number of the most influential in lowering levels of BOD. This type of research is true experimental design with one group pretest-posttest. The research material was Limnocharis flava. The result showed the levels of initial BOD rubber waste amounted to 299.5 mg / l, decreased levels of BOD of waste rubber after being treated with Limnocharis flava with a variety of clumps. Decreased levels of BOD rubber waste for each successive treatment ranging from 1, 2, 3, 4, and 5 clumps genjer ie 13.65%, 14, 52%, 15.6%, 20.94%, and 29.05%. Based on test results obtained linear regression for each treatment their effect on levels of BOD using genjer rubber waste. Treatment using Limnocharis flava much as 5 clump results BOD levels decrease the total of up to 29.05%. This value has not met the standard the maximum allowable levels of BOD. Need soaking clumps Limnocharis flava more stout and longer so that the rubber waste reduction in BOD levels in accordance with the quality standards required.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"6-10"},"PeriodicalIF":0.0,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69806699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yayuk Kustiningsih, Jujuk Anton Cahyono, Nur Rahmiati
Abstract: Examination of urine leukocyte count usually use urine samples during and urine examination is performed while still fresh because elements in the form of urine begins to break down within 2 hours. The purpose of this study was to determine the effect of storage time on the number of leukocytes urine checked immediately (0 hours) and were kept 30, 60, 90, 120, 150 and 180 minutes at room temperature. Types of Research true experiment to investigate the result of a treatment, research conducted on four samples of urine of patients with diabetes mellitus who meet several criteria, namely blood glucose levels> 200 mg / dl and urine amount leukositnya> 30 cells / mm3, calculated using the Improved Neubauer . The result showed a decrease in the number of stored urine leukocytes 30 minutes = 10%, 60 min = 25%, 90 min = 32%, 120 minutes = 39%, 150 minutes = 43%, and 180 minutes = 51%. Values obtained by linear regression test p = 0.034 (<α = 0.05). There is a significant relationship between duration of storage of urine with urine leukocyte counts were immediately checked (0 min) and stored 30, 60, 90, 120, 150 and 180 minutes at room temperature. Examination of urine leukocyte counts should be performed immediately or use the urine that is still in a fresh state.
{"title":"Pengaruh Lama Penyimpanan Urine pada Suhu Kamar terhadap Jumlah Leukosit Studi pada Penderita Diabetes Melitus","authors":"Yayuk Kustiningsih, Jujuk Anton Cahyono, Nur Rahmiati","doi":"10.31964/mltj.v2i1.25","DOIUrl":"https://doi.org/10.31964/mltj.v2i1.25","url":null,"abstract":"Abstract: Examination of urine leukocyte count usually use urine samples during and urine examination is performed while still fresh because elements in the form of urine begins to break down within 2 hours. The purpose of this study was to determine the effect of storage time on the number of leukocytes urine checked immediately (0 hours) and were kept 30, 60, 90, 120, 150 and 180 minutes at room temperature. Types of Research true experiment to investigate the result of a treatment, research conducted on four samples of urine of patients with diabetes mellitus who meet several criteria, namely blood glucose levels> 200 mg / dl and urine amount leukositnya> 30 cells / mm3, calculated using the Improved Neubauer . The result showed a decrease in the number of stored urine leukocytes 30 minutes = 10%, 60 min = 25%, 90 min = 32%, 120 minutes = 39%, 150 minutes = 43%, and 180 minutes = 51%. Values obtained by linear regression test p = 0.034 (<α = 0.05). There is a significant relationship between duration of storage of urine with urine leukocyte counts were immediately checked (0 min) and stored 30, 60, 90, 120, 150 and 180 minutes at room temperature. Examination of urine leukocyte counts should be performed immediately or use the urine that is still in a fresh state.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"11-16"},"PeriodicalIF":0.0,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: The Potential danger from mining activities is a worm infection. worm infection is often found in the community and are often considered harmless. This condition if left unchecked will lead to malnutrition, decreased endurance and work productivity. The aim of research to determine the risk factors and the incidence of worm infection on a traditional diamond miners in the district Cempaka Banjarbaru. This research is an analytic study with cross sectional design with a sample size of 60 people. The variables studied were the risk factors and the incidence of worm infection. The results of the study of risk factors normal nutritional status of respondents (46.7%), lack of knowledge level (63.3%); using Rescue Tool in work (63.3%); Good personal hygiene (51.7%), bowel habits are not eligible (60.0%) and worm infection (33.3%). The statistical test result with Che-Square test (X2), there was a significant relationship between risk factors and the incidence of worm disease in miners (p ˂ 0.05). Expected workers use personal protective equipment, health checked regularly and get a clean and healthy living behavior.
{"title":"Risiko Infeksi Kecacingan pada Penambang Intan Tradisional di Kecamatan Cempaka Kota Banjarbaru","authors":"E. Roebiakto, Yohanes Joko Supriyadi","doi":"10.31964/MLTJ.V2I1.29","DOIUrl":"https://doi.org/10.31964/MLTJ.V2I1.29","url":null,"abstract":"Abstract: The Potential danger from mining activities is a worm infection. worm infection is often found in the community and are often considered harmless. This condition if left unchecked will lead to malnutrition, decreased endurance and work productivity. The aim of research to determine the risk factors and the incidence of worm infection on a traditional diamond miners in the district Cempaka Banjarbaru. This research is an analytic study with cross sectional design with a sample size of 60 people. The variables studied were the risk factors and the incidence of worm infection. The results of the study of risk factors normal nutritional status of respondents (46.7%), lack of knowledge level (63.3%); using Rescue Tool in work (63.3%); Good personal hygiene (51.7%), bowel habits are not eligible (60.0%) and worm infection (33.3%). The statistical test result with Che-Square test (X2), there was a significant relationship between risk factors and the incidence of worm disease in miners (p ˂ 0.05). Expected workers use personal protective equipment, health checked regularly and get a clean and healthy living behavior.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.
{"title":"Mutu Bakteriologis Saus Tomat Pentol di Banjarbaru","authors":"Ratih Dewi Dwiyanti, Leka Lutpiatina","doi":"10.31964/MLTJ.V2I1.31","DOIUrl":"https://doi.org/10.31964/MLTJ.V2I1.31","url":null,"abstract":"Abstract: The food was used as a complementary flavor sauces include snacks bulb. Dressing materials according to SNI 01-7388-2009 must comply with the requirements of the parameter Staphylococcus aureus, MPN (Most probable number) Coliform, TPC (total plate count) and molds. This study aims to determine the percentage of tomato sauce snacks bulb in the area around the intersection of four Banjarbaru who do not qualify SNI 01-7388-2009 of parameters MPN Coliform and Staphylococcus aureus. The results showed MPN Coliform tomato sauce ranges from 0-240 and Staphylococcus aureus 5 x 101 to 6.5 x 103. Concluded percentage tomato sauce snacks bulb that does not qualify SNI 01-7388-2009 parameter is 60% MPN Coliform and Staphylococcus aureus 80%. Suggestions to determine the value of TPC (total plate count) and molds and other chemical parameters of tomato sauce.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fasciolopsis buski is a health problem that is difficult to eradicate. Fasciolopsis buski infection is caused by ingested metacercariae that live on aquatic plants. One factor that supports the transmission mechanism of Fasciolopsis buski, of which there are still some people, especially children who have a habit of eating Nymphea sp. The study aims to determine the relationship with lotus fruit consumption habits with Fasciolopsis buski infection. The type of research is analytic survey with cross sectional approach, the study samples are 69 students of Sungai Papuyu primary school consists of 38 men and 31 women, the technique of sampling is random sampling (simple random), research instruments are using questionnaires and microscopic examination of worm eggs, data analysis is using the Chi-square. Students are accustomed to eating Nymphea sp of crude (not good) are 31 people (44.93%), and are not familiar (well) are to 38 people (55.7%). A total of 7 people (10:14%) are infected Fasciolopsis buski and 62 (89.86%) are not. Students who are infected Fasciolopsis buski entirely used to eat fruit lotus. Based on the statistical test Chi-Square, there is a relationship between the habit of eating Nymphea sp with lotus Fasciolopsis buski infection with the value of Fisher's Exact Test 0,002 less than the value ? of 0.05. The habit of eating Nymphea sp are associated with infections to the students.
{"title":"Konsumsi Buah Teratai (Nymphea sp) Sebagai Determinan Terjadinya Fasciolopsis Buski pada Anak","authors":"M. Muslim, ri Rifqoh, R. Irwandi","doi":"10.31964/mltj.v2i1.33","DOIUrl":"https://doi.org/10.31964/mltj.v2i1.33","url":null,"abstract":"Fasciolopsis buski is a health problem that is difficult to eradicate. Fasciolopsis buski infection is caused by ingested metacercariae that live on aquatic plants. One factor that supports the transmission mechanism of Fasciolopsis buski, of which there are still some people, especially children who have a habit of eating Nymphea sp. The study aims to determine the relationship with lotus fruit consumption habits with Fasciolopsis buski infection. The type of research is analytic survey with cross sectional approach, the study samples are 69 students of Sungai Papuyu primary school consists of 38 men and 31 women, the technique of sampling is random sampling (simple random), research instruments are using questionnaires and microscopic examination of worm eggs, data analysis is using the Chi-square. Students are accustomed to eating Nymphea sp of crude (not good) are 31 people (44.93%), and are not familiar (well) are to 38 people (55.7%). A total of 7 people (10:14%) are infected Fasciolopsis buski and 62 (89.86%) are not. Students who are infected Fasciolopsis buski entirely used to eat fruit lotus. Based on the statistical test Chi-Square, there is a relationship between the habit of eating Nymphea sp with lotus Fasciolopsis buski infection with the value of Fisher's Exact Test 0,002 less than the value ? of 0.05. The habit of eating Nymphea sp are associated with infections to the students.","PeriodicalId":76135,"journal":{"name":"Medical laboratory technology","volume":"2 1","pages":"25-30"},"PeriodicalIF":0.0,"publicationDate":"2016-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69807221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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