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Phytochemical investigation and in vitro cytotoxicity of Phlogacanthus thyrsiformis (Roxb. Ex Hardw.) Mabb. against Dalton’s Lymphoma Ascites (DLA) cells Phlogacanthus thyrsiformis (Roxb. Ex Hardw.) Mabb.对道尔顿淋巴瘤腹水(DLA)细胞的植物化学研究和体外细胞毒性
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-22 DOI: 10.1007/s13596-023-00731-0
Mary Devi, Akalesh Kumar Verma, Namram Sushindrajit Singh, Jumi Das, Kasturi Dutta, Munmi Gogoi, Diksha Dutta

Phlogacanthus thyrsiformis, a valuable medicinal plant frequently employed by ethnic communities for various health concerns, has shown potential against cancer cell lines in lab tests. However, its effectiveness against Dalton’s Lymphoma (DL) cells remains unknown. In our current study, we aim to investigate the phytochemical constituents, antioxidant properties, and cytotoxic effects of P. thyrsiformis extract against the DL cell line. Additionally, we studied its apoptotic effects through fluorescent staining and Scanning Electron Micrograph (SEM) studies. The plant extract revealed the presence of various important phytochemicals such as carbohydrates, phenols, polyphenols, flavonoids, tannins, terpenoids, triterpenoids, alkaloids, and saponins in various degrees. The analysis revealed that the total phenol content and total flavonoid content were present in substantial quantities, suggesting a strong correlation with its potent antioxidant abilities and also showing close IC50 with the reference, ascorbic acid. Effective growth inhibition of DL cells was observed at different treatment concentrations without affecting normal cells. The occurrence of such cytotoxic effects could be corroborated to its apoptotic-inducing properties. Apoptotic cell death was observed in a dose-dependent manner and further morphological changes were evidenced through SEM studies. Our study thus substantiates that P. thyrsiformis is a potent plant with a reservoir of active phytochemicals and is a natural source of antitumor agents against DL cells.

Phlogacanthus thyrsiformis 是一种珍贵的药用植物,经常被少数民族用来治疗各种健康问题。然而,它对道尔顿淋巴瘤(DL)细胞的有效性仍然未知。在我们目前的研究中,我们旨在调查胸苷提取物的植物化学成分、抗氧化特性以及对 DL 细胞系的细胞毒性作用。此外,我们还通过荧光染色和扫描电子显微镜(SEM)研究了其凋亡效应。植物提取物中含有各种重要的植物化学物质,如不同程度的碳水化合物、酚类、多酚类、黄酮类、单宁、萜类、三萜类、生物碱和皂苷。分析表明,总酚含量和总黄酮含量都很高,这表明它具有很强的抗氧化能力,其 IC50 值也与抗坏血酸接近。在不同的处理浓度下,都能有效抑制 DL 细胞的生长,而不影响正常细胞。这种细胞毒性效应的出现可能与其诱导细胞凋亡的特性有关。凋亡细胞的死亡呈剂量依赖性,扫描电镜研究进一步证实了其形态学变化。因此,我们的研究证实,P. thyrsiformis 是一种有效的植物,含有大量活性植物化学物质,是抗 DL 细胞的天然抗肿瘤剂来源。
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引用次数: 0
Evaluation of a new topical cream to psoriasis treatment: randomized clinical trial 评估治疗牛皮癣的新型外用药膏:随机临床试验
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-17 DOI: 10.1007/s13596-023-00719-w
Elizabeth Benites, Esmeralda Carrillo, Martha Heras, Doménica Bourne

The aim of this study is to develop a new topical cream for psoriasis and to test its results. Experimental clinical trial. Phase I: hypersensitivity test in 100 healthy volunteers. Phase II: 120 patients, prospective, analytical differential study in hospital. In phase I, 99% health persons did not show any reaction. In phase II, the 120 patients selected in two groups, group A-new cream and group B-corticosteroid, the paired samples calculated T-value in group A was: 19.144 for erythema, 6.725 in infiltrate, 12.475 in desquamation and in pruritus 10.863, PASI 14.662 and BSA 19.083 which are higher than 1.96 the result was 0.000 less than P 0.05 highly significant after treatment group B control group, showed the T values, erythema 7.590, infiltrate 10.573, desquamation 7.311, pruritus 3.615, PASI 2.631, BSA 4.411. The T-values which are higher than 1.96 were 0.000 less than P 0.05 highly significant after treatment. In the Pearson correlation for cases and controls, association values were observed for SBA 0.042 before treatment and 0.008, after treatment, in PASI 0.021, before treatment and 0.008 after treatment. The results examined by hypotheses and cross-tabulations are highly significant in groups A and B separately, and when group A is tested against group B by means of cross-tabulations, they show differences after treatment. In the logarithmic regression for the associated risk factors, the odds ratio values of BMI 1.113>OR 1 and urea 1.439>OR 1. The elevation of these two parameters may affect the exposed group. When comparing the group, A and group B, the symptoms of erythema, infiltrate, desquamation, pruritus, PASI index and SBA were found to decrease with the group A/new cream after treatment. Further studies in larger populations are suggested, the new cream has been shown to reduce the clinical symptoms of psoriasis.

这项研究的目的是开发一种新的牛皮癣外用药膏,并测试其效果。实验性临床试验。第一阶段:对 100 名健康志愿者进行过敏试验。第二阶段:120 名患者,在医院进行前瞻性分析性差异研究。在第一阶段,99% 的健康人未出现任何反应。在第二阶段,120 名患者被分为两组,A 组-新型药膏,B 组-皮质类固醇,A 组的配对样本计算 T 值为:红斑 19.144,浸润 6.725,脱屑 12.475,瘙痒 10.863,PASI 14.662,BSA 19.0。B 对照组的 T 值为:红斑 7.590,浸润 10.573,脱屑 7.311,瘙痒 3.615,PASI 2.631,BSA 4.411。治疗后的 T 值大于 1.96,小于 P 0.05,具有高度显著性。在病例和对照组的皮尔逊相关性中,观察到治疗前 SBA 为 0.042,治疗后为 0.008;治疗前 PASI 为 0.021,治疗后为 0.008。通过假设和交叉表检验的结果在 A 组和 B 组中分别具有高度显著性,当通过交叉表检验 A 组与 B 组时,它们在治疗后显示出差异。在相关危险因素的对数回归中,BMI1.113>OR 1、尿素1.439>OR 1的几率值,这两个参数的升高可能会影响暴露组。在对 A 组和 B 组进行比较时,发现治疗后红斑、浸润、脱屑、瘙痒、PASI 指数和 SBA 等症状均随 A 组/新乳膏的使用而减少。建议在更大的人群中开展进一步研究,新药膏已被证明能减轻银屑病的临床症状。
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引用次数: 0
Isolation and evaluation of antiviral plant compounds against respiratory disease-causing viruses: a review 针对呼吸道致病病毒的抗病毒植物化合物的分离与评估:综述
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-16 DOI: 10.1007/s13596-023-00723-0
Thamby Rajah Mahendran, Binsin Cynthia, Ganapaty Manickavasagam, John J. Thambirajah, Maheswaran Solayappan, Thirumalai Komala

Influenza viruses are major concern worldwide due to their fast ability to spread, and causing the respiratory illness of varying severity. The rate of travel, urbanization and socializing among the people are the major factors for the spread. The coronaviruses causing the contagious respiratory illness are similar to influenza viruses. The ongoing COVID-19 pandemic serves as an example on how a single influenza virus can greatly disrupt the economy and social activity worldwide on unprecedented scale. The emergence of the new variants also presents threat for the development of drugs and/or vaccines for the treatment of viral infection. Hence, researchers worldwide have been focusing into plant compounds to identify effective bioactive compound which could be developed into therapeutic antiviral drugs against influenza viruses and coronaviruses. The aim of this paper is to summarize the bioactive compounds isolated from plants that exhibit potential against influenza viruses and coronaviruses. This paper also investigates the techniques used in isolation, characterisation and elucidation of antiviral activity of plant compounds in the evaluation and application of such compounds for therapeutic purpose.

流感病毒具有快速传播能力,可引起严重程度不同的呼吸道疾病,因此是全球关注的主要问题。旅行率、城市化和人们之间的社交活动是传播的主要因素。引起传染性呼吸道疾病的冠状病毒与流感病毒相似。正在发生的 COVID-19 大流行就是一个例子,说明单一流感病毒如何以前所未有的规模极大地破坏全球经济和社会活动。新变种的出现也对治疗病毒感染的药物和/或疫苗的开发构成了威胁。因此,世界各地的研究人员一直在重点研究植物化合物,以找出有效的生物活性化合物,并将其开发成治疗流感病毒和冠状病毒的抗病毒药物。本文旨在总结从植物中分离出的具有抗流感病毒和冠状病毒潜力的生物活性化合物。本文还研究了植物化合物的分离、表征和抗病毒活性阐明技术,以评估和应用此类化合物达到治疗目的。
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引用次数: 0
Protective effect of combined ginger and pumpkin seed extracts on cyclophosphamide-induced hepatotoxicity in rats 生姜和南瓜籽联合提取物对环磷酰胺诱导的大鼠肝毒性的保护作用
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-11 DOI: 10.1007/s13596-023-00721-2
Hamed Haddad Kashani, Maryam Taheri, Zeinab Qotbi, Abolfazl Zahedi, Fatemeh Izadpanah, Hossein Nikzad

Cyclophosphamide is an alkylating agent that is widely used in cancer therapy. An important complication of this drug is hepatotoxicity. The hepatic prophylactic effects of ginger and pumpkin seeds were observed due to antioxidant effect. In the current study, the effects of ginger, pumpkin seed extracts, and their combination on cyclophosphamide-induced hepatotoxicity in rats were investigated. In this intervention study, a total of 70 male rats were randomly divided into 10 groups. The blood was taken after 2 weeks of treatment. Liver enzyme levels including ALT, AST and MDA were measured. Sections of the livers of all groups were provided and then histologically evaluated. The data obtained were analyzed using SPSS software version 16. A significant increase in liver enzymes was observed in rats treated with cyclophosphamide compared to other groups. Liver enzyme levels were decreased in rats treated with ginger, pumpkin seed and combined extracts (P < 0.05). Histological examination of liver samples treated with ginger, pumpkin seeds and combined extracts showed a significant reduction in liver damage compared to the cyclophosphamide-treated group (P < 0.05). Ginger, pumpkin seeds and combined extracts could improve biochemical parameters such as AST and ALT and pathological damage in the liver of cyclophosphamide-treated rats.

环磷酰胺是一种广泛用于癌症治疗的烷化剂。这种药物的一个重要并发症是肝毒性。生姜和南瓜籽的肝脏预防作用是通过抗氧化作用观察到的。本研究调查了生姜、南瓜籽提取物及其组合对环磷酰胺诱导的大鼠肝毒性的影响。在这项干预研究中,共有 70 只雄性大鼠被随机分为 10 组。治疗 2 周后采血。测量肝酶水平,包括谷丙转氨酶、谷草转氨酶和 MDA。提供所有组的肝脏切片,然后进行组织学评估。获得的数据使用 SPSS 软件 16 版进行分析。与其他组相比,接受环磷酰胺治疗的大鼠肝酶水平明显升高。用生姜、南瓜籽和联合提取物处理的大鼠肝酶水平下降(P < 0.05)。用生姜、南瓜籽和混合提取物处理的肝脏样本的组织学检查显示,与环磷酰胺处理组相比,生姜、南瓜籽和混合提取物处理组的肝脏损伤显著减少(P < 0.05)。生姜、南瓜籽和联合提取物可以改善环磷酰胺治疗大鼠肝脏的生化指标,如 AST 和 ALT,以及病理损伤。
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引用次数: 0
Evaluating the bioactivity and toxicity of Siparuna guianensis Aublet (Siparunaceae) leaf extracts in zebrafish 评估 Siparuna guianensis Aublet(Siparunaceae)叶提取物在斑马鱼体内的生物活性和毒性
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-07 DOI: 10.1007/s13596-023-00722-1
Maria de Fátima Santos, William Franco Carneiro, Bárbara do Carmo Rodrigues Virote, Kiara Cândido Duarte da Silva, Tassia Flavia Dias Castro, Aline Pereira Coli, Luis David Solis Murgas, Marcos Ferrante, Manuel Losada Gavilanes, Elisangela Elena Nunes Carvalho

Siparuna guianensis, a traditionally utilized aromatic plant known as negramina, is recognized for its anti-inflammatory, analgesic, and antipyretic properties. The present study explores the toxicity, neutrophil migration, tail fin regeneration, and antioxidant effects of various S. guianensis leaf extracts on zebrafish embryos and larvae. Methanol (ME), 70% ethyl alcohol (HE), and distilled water (AE) were used to prepare extracts from the leaves, and the presence of phenolic compounds was confirmed. Additionally, the antioxidant activity index (AAI) was evaluated. Using a zebrafish model, toxicity and teratogenicity were assessed over a 120-h period with concentrations varying from 0.08 to 10 mg/ml. Non-lethal concentrations were used to study neutrophil migration, tail fin regeneration, cell apoptosis, and antioxidant enzyme activity, which included enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST). The extracts, which exhibited high AAI values, were found to contain phenolic compounds. AE proved toxic at concentrations from 2.5 to 10 mg/ml, whereas HE and ME showed toxicity at concentrations between 0.62 and 10 mg/ml. Interestingly, AE-treated larvae did not show any malformations. Despite a reduction in neutrophil migration observed with all tested extracts, they did not promote fin regeneration. The activity of SOD and CAT enzymes remained unaffected by the extracts. Unlike HE and ME, AE did not induce apoptosis in cells. In summary, the leaf extracts of S. guianensis (AE, HE, ME) are rich in phenolic compounds, demonstrate a reduction in neutrophil migration, and possess high AAI values.

Graphical abstract

Siparuna guianensis是一种传统的芳香植物,被称为 "negramina",具有消炎、镇痛和解热的功效。本研究探讨了各种 S. guianensis 叶提取物对斑马鱼胚胎和幼体的毒性、中性粒细胞迁移、尾鳍再生和抗氧化作用。采用甲醇(ME)、70%乙醇(HE)和蒸馏水(AE)制备叶片提取物,并确认其中含有酚类化合物。此外,还评估了抗氧化活性指数(AAI)。利用斑马鱼模型,在浓度为 0.08 至 10 mg/ml 的 120 小时内评估了毒性和致畸性。非致死浓度用于研究中性粒细胞迁移、尾鳍再生、细胞凋亡和抗氧化酶活性,其中包括超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽-S-转移酶(GST)等酶。提取物的 AAI 值很高,其中含有酚类化合物。AE 的毒性浓度为 2.5 至 10 毫克/毫升,而 HE 和 ME 的毒性浓度为 0.62 至 10 毫克/毫升。有趣的是,经 AE 处理的幼虫没有出现任何畸形。尽管所有测试提取物都能减少中性粒细胞的迁移,但它们并不能促进鳍的再生。SOD 和 CAT 酶的活性不受提取物的影响。与 HE 和 ME 不同,AE 不会诱导细胞凋亡。总之,S. guianensis 的叶提取物(AE、HE、ME)含有丰富的酚类化合物,能减少中性粒细胞的迁移,并具有较高的 AAI 值。
{"title":"Evaluating the bioactivity and toxicity of Siparuna guianensis Aublet (Siparunaceae) leaf extracts in zebrafish","authors":"Maria de Fátima Santos,&nbsp;William Franco Carneiro,&nbsp;Bárbara do Carmo Rodrigues Virote,&nbsp;Kiara Cândido Duarte da Silva,&nbsp;Tassia Flavia Dias Castro,&nbsp;Aline Pereira Coli,&nbsp;Luis David Solis Murgas,&nbsp;Marcos Ferrante,&nbsp;Manuel Losada Gavilanes,&nbsp;Elisangela Elena Nunes Carvalho","doi":"10.1007/s13596-023-00722-1","DOIUrl":"10.1007/s13596-023-00722-1","url":null,"abstract":"<div><p><i>Siparuna guianensis</i>, a traditionally utilized aromatic plant known as negramina, is recognized for its anti-inflammatory, analgesic, and antipyretic properties. The present study explores the toxicity, neutrophil migration, tail fin regeneration, and antioxidant effects of various <i>S</i>. <i>guianensis</i> leaf extracts on zebrafish embryos and larvae. Methanol (ME), 70% ethyl alcohol (HE), and distilled water (AE) were used to prepare extracts from the leaves, and the presence of phenolic compounds was confirmed. Additionally, the antioxidant activity index (AAI) was evaluated. Using a zebrafish model, toxicity and teratogenicity were assessed over a 120-h period with concentrations varying from 0.08 to 10 mg/ml. Non-lethal concentrations were used to study neutrophil migration, tail fin regeneration, cell apoptosis, and antioxidant enzyme activity, which included enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST). The extracts, which exhibited high AAI values, were found to contain phenolic compounds. AE proved toxic at concentrations from 2.5 to 10 mg/ml, whereas HE and ME showed toxicity at concentrations between 0.62 and 10 mg/ml. Interestingly, AE-treated larvae did not show any malformations. Despite a reduction in neutrophil migration observed with all tested extracts, they did not promote fin regeneration. The activity of SOD and CAT enzymes remained unaffected by the extracts. Unlike HE and ME, AE did not induce apoptosis in cells. In summary, the leaf extracts of <i>S</i>. <i>guianensis</i> (AE, HE, ME) are rich in phenolic compounds, demonstrate a reduction in neutrophil migration, and possess high AAI values.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 2","pages":"569 - 582"},"PeriodicalIF":1.8,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135475703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Methotrexate-induced intestine and nephrotoxicity attenuated by Andrographis paniculata via ameliorating oxidative stress, inflammation and apoptosis 穿心莲通过改善氧化应激、炎症和细胞凋亡减轻甲氨蝶呤引起的肠毒性和肾毒性
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-07 DOI: 10.1007/s13596-023-00720-3
Manisha Parthasarathy, Sabina Evan Prince

The therapeutical benefits of methotrexate (MTX) antifolate agents are non-negligible. However, their ability to cause severe intestinal and nephrotoxicity initiates burden on the patient’s life quality. Andrographis paniculata, “kings of bitter” with anti-inflammatory, anti-bacterial, detoxicant and anti-viral properties, is a preferred Asian medicinal plant for treating various disease ailments. Our study aims to determine the potential of aqueous leaf extract of A. paniculata (ALEAP) against MTX-induced intestinal and nephrotoxicity. Rats (n = 6) in 5 groups were allocated. Group 1 (normal control), group 2 (MTX − 20 mg/kg; i.p. single administration, 9th day), group 3 (MTX + ALEAP (500 mg/kg; p.o. for 10 days)), group 4 (MTX + Silymarin (100 mg/kg; p.o. for 10 days)) and group 5 (ALEAP alone for 10 days). The rats were assessed for significant alterations in renal markers, intestine and renal antioxidant levels, histopathology, cytokine levels (TNF-α, IL-6, IL-10), and apoptosis marker expression range (caspase 3, 8, BCL-2). ALEAP attenuates elevated renal markers; intestine and renal MDA, TNF-α, IL-6, caspase 3 & 8, and histopathological modifications. ALEAP restored intestine and renal antioxidant enzymes, IL-10, and BCL-2. The aqueous leaf extract of A. paniculata produced a significant outcome by maintaining intestine and kidney function and preventing cellular damage.

Graphical abstract

甲氨蝶呤(MTX)抗叶酸药在治疗上的益处是不可忽视的。然而,它们会引起严重的肠道和肾毒性,给患者的生活质量带来负担。穿心莲是 "苦味之王",具有消炎、抗菌、解毒和抗病毒的特性,是治疗各种疾病的首选亚洲药用植物。我们的研究旨在确定 A. paniculata 的水性叶提取物(ALEAP)对 MTX 引起的肠道和肾毒性的潜在作用。研究将大鼠(n = 6)分为 5 组。第1组(正常对照组)、第2组(MTX - 20 mg/kg; i.p. 单次给药,第9天)、第3组(MTX + ALEAP (500 mg/kg; p.o. 10天))、第4组(MTX + 水飞蓟素 (100 mg/kg; p.o. 10天))和第5组(单用ALEAP,10天)。对大鼠肾脏标志物、肠道和肾脏抗氧化剂水平、组织病理学、细胞因子水平(TNF-α、IL-6、IL-10)和细胞凋亡标志物表达范围(caspase 3、8、BCL-2)的显著变化进行了评估。ALEAP 可减轻升高的肾脏标记物、肠道和肾脏 MDA、TNF-α、IL-6、caspase 3 & 8 以及组织病理学改变。ALEAP 可恢复肠道和肾脏的抗氧化酶、IL-10 和 BCL-2。A.paniculata的水性叶提取物通过维持肠道和肾脏功能以及防止细胞损伤产生了显著效果。 图摘
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引用次数: 0
Salvia africana-lutea L.: a review of ethnobotany, phytochemistry, pharmacology applications and future prospects Salvia africana-lutea L.:民族植物学、植物化学、药理学应用和未来前景综述
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-06 DOI: 10.1007/s13596-023-00726-x
Chinonso Anthony Ezema, Rita Ngozi Aguchem, Emmanuel Chigozie Aham, Wisdom Favour Chinedu Ezeorba, Innocent Uzochukwu Okagu, Timothy Prince Chidike Ezeorba

Plants are nature’s reserve for vitality and health-boosting agents. Despite ever-rising interest and research on plant medicinal chemistry, many stones are still being left unturned. Moreover, many traditional medicinal plants are yet to be discovered or functionally characterized. This study presented an up-to-date review of a poorly explored member of the Salvia genus indigenous to Africa—Salvia africana-lutea L. (synonymous with Salvia aurea L.) with details on its geographical distribution, ethnobotany, and pharmacological applications. We reviewed all literature published on Salvia africana-lutea up to January 2023, retrieved from PubMed, Scopus, and ScienceDirect as primary databases and google scholar as the secondary source. From our literature search, we found 38 documents published on S. africana-lutea, despite the popularity of the Salvia genus as a medicinal plant (having over 15,000 articles published to date). From the retrieved literature, only a few studies focused on exploiting the ethnobotanical features of the plants, such as the morphology, flowering and existence, and nature of its trichomes. Some studies have reported S. africana-lutea as an excellent source of essential oils trapped within their leaf trichomes with numerous phytochemicals and bioactivities. Other studies have reported some interesting pharmacological activities of plant extracts and isolated phytochemicals, such as their antimicrobial, anti-oxidative, analgesic, antipyretic, anticancer, cytotoxic, and other bioactivities. We identified some limitations of the few published studies, highlighting future research needs that should draw more scientific interest to foster more study on this under-explored and valuable plant species of Salvia, to harness its medicinal and industrial potential fully.

植物是大自然蕴藏的生命力和健康促进剂。尽管人们对植物药用化学的兴趣与日俱增,研究也在不断深入,但仍有许多问题尚未解决。此外,还有许多传统药用植物尚未被发现或在功能上尚未定性。本研究对非洲土生土长的丹参属植物--Salvia africana-lutea L.(与 Salvia aurea L.同义)进行了最新综述,详细介绍了其地理分布、民族植物学和药理应用。我们查阅了截至 2023 年 1 月所有关于非洲鼠尾草的文献,主要数据库为 PubMed、Scopus 和 ScienceDirect,次要来源为 google scholar。在文献检索中,我们发现了 38 篇关于 S. africana-lutea 的文献,尽管丹参属植物作为药用植物很受欢迎(迄今已发表超过 15,000 篇文章)。在检索到的文献中,只有少数研究侧重于利用植物的民族植物学特征,如形态、花期、存在时间及其毛状体的性质。一些研究报告称,S. africana-lutea 是一种极好的精油来源,其叶片毛状体中含有大量植物化学物质和生物活性物质。其他研究报告了植物提取物和分离的植物化学物质的一些有趣的药理活性,如抗菌、抗氧化、镇痛、解热、抗癌、细胞毒性和其他生物活性。我们指出了已发表的少数研究的一些局限性,并强调了未来的研究需求,这些需求应引起更多科学界的兴趣,以促进对丹参这一开发不足的珍贵植物物种的更多研究,从而充分利用其药用和工业潜力。
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引用次数: 0
A new model for estimating basal metabolic rate (BMR) using temperament in traditional Persian medicine (TPM) 利用传统波斯医学(TPM)中的气质估算基础代谢率(BMR)的新模型
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-11-01 DOI: 10.1007/s13596-023-00715-0
Mahdi Kafaee, Elahe Daviran, Sonia Heydari, Shahriar Gharibzadeh

The estimation of basal metabolic rate is an important parameter in treating metabolic diseases and prescribing diet regimes. Some equations and analyses have been presented for a reliable estimate of basal metabolic rate. Although these estimates are plausible, they are all subject to errors due to unknown factors. The error reduction of this estimation is under debate. Based on some recent studies, significant similarities have been reported between factors affecting basal metabolic rate in modern medicine and temperament in traditional Persian medicine. Thus, our objective is to propose a new model to increase the accuracy of basal metabolic rate estimation based on temperament in traditional Persian medicine. Based on the available studies about the relationship between basal metabolic rate and temperament, we hypothesize that adding a new factor (warmness/ coldness) to common regression formulas of the basal metabolic rate may reduce the errors. In this article, we propose suggestions to confirm this model.

基础代谢率的估算是治疗代谢性疾病和制定饮食方案的一个重要参数。为了可靠地估算基础代谢率,已经提出了一些方程和分析方法。尽管这些估算结果可信,但都会因未知因素而产生误差。如何减少这种估算的误差还存在争议。根据最近的一些研究,现代医学中影响基础代谢率的因素与传统波斯医学中的气质之间存在着显著的相似性。因此,我们的目标是提出一个新模型,以提高基于传统波斯医学中气质的基础代谢率估算的准确性。根据有关基础代谢率与性情之间关系的现有研究,我们假设在基础代谢率的常见回归公式中加入一个新因素(冷/暖)可能会减少误差。在本文中,我们将提出一些建议来证实这一模型。
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引用次数: 0
Modulatory effect of Justicia secunda leaf extract on hematological status, lipid profile, liver function and oxidative stress in Wistar rats 大叶女贞叶提取物对 Wistar 大鼠血液状态、血脂、肝功能和氧化应激的调节作用
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-10-30 DOI: 10.1007/s13596-023-00716-z
Mary Remi Bose Oyewale, Oluwatooyin Faramade Osundahunsi, Olugbenga Olufemi Awolu

Justicia secunda is a therapeutic plant with acclaimed beneficial health effects including blood-boosting potential. However, scientific evidence from in vivo analyses to buttress such claims have not been exhaustively provided. Herein, we investigated the effect of J. secunda leaf extract on organs, hematology, lipid profile and endogenous antioxidant activity in male rats grouped into seven groups of five animals each. Tween-dissolved extract, in various concentrations (10 mg/kg body weight (bwt), 100 mg/kg bwt, 1000 mg/kg bwt, 1600 mg/kg bwt, 2900 mg/kg bwt and 5000 mg/kg bwt), was orally administered to group II–VII, respectively, whereas group one (fed 20% tween solution only) served as the control. After 28 days, the rats were sacrificed while blood was taken for hematological and biochemical assays. The liver, kidney and spleen were also isolated for histopathological analyses. In the sub-chronic toxicity analysis, the LD50 was above 5000 mg/kg bwt. Packed cell volume, red blood cell and hemoglobin increased significantly (p < 0.05) in group II and IV compared with the other administered groups. No significant difference was observed in the activity of ALT, AST, ALP and creatinine (p < 0.05) across the groups administered 10, 100, 1000 mg/kg bwt extract relative to the control. The activity of superoxide dismutase and malondialdehyde was higher in the administered groups than the control. Cholesterol, triglyceride and high-density lipoprotein-cholesterol level was increased in a dose dependent manner in the treated rats. However, low density lipoprotein-cholesterol level was reduced.

Graphical abstract

Justicia secunda 是一种治疗植物,其有益健康的功效广受赞誉,其中包括促进血液循环的潜力。然而,目前还没有详尽的体内分析科学证据来证实这种说法。在此,我们研究了鸦胆子叶提取物对雄性大鼠器官、血液学、血脂和内源性抗氧化活性的影响,雄性大鼠分为七组,每组五只。第二至第七组分别口服不同浓度(10 毫克/千克体重、100 毫克/千克体重、1000 毫克/千克体重、1600 毫克/千克体重、2900 毫克/千克体重和 5000 毫克/千克体重)的吐温溶解提取物,而第一组(仅喂食 20% 吐温溶液)作为对照。28 天后,大鼠被处死,取血进行血液和生化检测。肝脏、肾脏和脾脏也被分离出来进行组织病理学分析。在亚慢性毒性分析中,半数致死剂量高于 5000 毫克/千克体重。与其他给药组相比,第 II 组和第 IV 组的包装细胞体积、红细胞和血红蛋白显著增加(p < 0.05)。与对照组相比,10、100、1000 毫克/千克体重提取物组的谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷草转氨酶(ALP)和肌酐的活性没有明显差异(p < 0.05)。给药组的超氧化物歧化酶和丙二醛活性高于对照组。受试大鼠的胆固醇、甘油三酯和高密度脂蛋白胆固醇水平呈剂量依赖性升高。图解摘要
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引用次数: 0
A comparative study of biological and chemical composition of African spinach (Amaranthus cruentus) extract: an approach in drug formulation, food and nutrition 非洲菠菜(Amaranthus cruentus)提取物的生物和化学成分比较研究:一种药物制剂、食品和营养方法
IF 1.8 Q3 PHARMACOLOGY & PHARMACY Pub Date : 2023-10-24 DOI: 10.1007/s13596-023-00718-x
Great Iruoghene Edo, Favour Ogheneoruese Onoharigho

African spinach (Amaranthus cruentus) is an edible-leaved member of the pigweed family grown widely in Africa as a cooked green vegetable. This research was aimed to investigate and evaluate the phytochemical, Proximate, FTIR, GC–MS and antibacterial properties in connection with health, safety, food and nutritional benefits of ethanol and hexane extract of African spinach leaves. African spinach leaves were extracted using ethanol and hexane. The aqueous ethanol and hexane extracts of African spinach leaves were used for the assessment of antibacterial activity by the agar well diffusion method. The leaves extracts showed the existence of bioactive compounds such as alkaloids, phenols, anthraquinones, cardiac glycosides, flavonoids, tannins, steroids, carbohydrates, proteins, terpenoids and fixed oils and fats. The GC–MS assessment of the extract showed the existence of bioactive compounds. Different concentrations (50, 100 and 200 mg/ml) of African spinach leaves extracts against Shigella specie, Salmonella specie, Staphylococcus aureus and Escherichia coli demonstrated high level of inhibitions. The findings in this current research showed that African spinach contain better consumable quality. The bioactive compounds are used for the treatment of different diseases such as bacterial infection, cancer, diabetic and inflammation which confirmed their effectiveness in producing traditional medicine.

非洲菠菜(Amaranthus cruentus)是一种可食用的猪笼草科植物,作为熟食绿色蔬菜在非洲广泛种植。本研究旨在调查和评估非洲菠菜叶乙醇和正己烷提取物的植物化学成分、物化性质、傅立叶变换红外光谱、气相色谱-质谱和抗菌特性,以及其对健康、安全、食品和营养的益处。非洲菠菜叶是用乙醇和正己烷提取的。非洲菠菜叶的乙醇和正己烷水提取物用于琼脂井扩散法评估抗菌活性。叶片提取物显示存在生物活性化合物,如生物碱、酚类、蒽醌类、强心甙类、黄酮类、单宁类、甾体类、碳水化合物、蛋白质、萜类化合物和固定油脂。提取物的气相色谱-质谱(GC-MS)评估表明,其中存在生物活性化合物。不同浓度(50、100 和 200 毫克/毫升)的非洲菠菜叶提取物对志贺氏杆菌、沙门氏菌、金黄色葡萄球菌和大肠杆菌都有很强的抑制作用。目前的研究结果表明,非洲菠菜含有更好的食用品质。这些生物活性化合物可用于治疗不同的疾病,如细菌感染、癌症、糖尿病和炎症,这证实了它们在生产传统药物方面的有效性。
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引用次数: 0
期刊
Advances in Traditional Medicine
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