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Substantiation and Development of Bakery Products Enriched with Arctic Vegetable Raw Materials 富含北极蔬菜原料的烘焙产品的研制
Q Engineering Pub Date : 2022-06-21 DOI: 10.29141/2500-1922-2022-7-2-5
Ludmila N. Burakova, D. Plotnikov
A man implements the development programs of the Arctic territory including the Yamalo-Nenets Autonomous District (Yamalo-Nenets Autonomous Okrug) actively. One of the development program goals of the Yamalo-Nenets Autonomous District is to ensure the food security of the region and the healthy lifestyle formation of the population aimed at preventing diseases. According to Rospotrebnadzor, in recent years infectious diseases have become widespread in the region due to the population immunodeficiency. A man decided to develop a complex dietary supplement with immunomodulatory properties and the feasibility to introduce it into the product of mass production. A group of bakery products was the basis of the functional product. The article presents the study results of bakery products enriched with plant raw materials – cinnamon rose (Fructus Rosae) and shiksha (black crowberry, Empetrii nigri herba) growing in the Yamalo-Nenets Autonomous District. The research aim is to substantiate the development of bakery products of mass demand enriched with a complex food additive based on Arctic plant raw materials. The preparation technology includes the main stages: sorting raw materials; grinding dry concentrates; heating it in a bioreactor and packaging. The researchers used raw materials in dry form: cinnamon rose hips – TU 10.89.19-036-14721358-2017; shiksha (black crowberry) – TU 10.89.19-086-14721358-2017). The organoleptic and physico-chemical analysis of the resulting product showed its compliance with the current regulations for food products. A positive assessment in terms of safety and compliance with functional properties enables to qualify an additive from plant raw materials as functional food ingredients – GOST R 54059-2010 “Functional Food Products. Functional Food Ingredients. Classification and General Requirements: National Standard of the Russian Federation”. The bakery product itself refers to products enriched with functional food ingredients – GOST R 55577-2013 “Specialized and Functional Food Products. Information on Distinctive Features and Effectiveness: National Standard of the Russian Federation”.
一名男子积极实施包括亚马尔-涅涅茨自治区(亚马尔-涅涅茨自治区)在内的北极地区的发展计划。亚马尔-涅涅茨自治区的发展方案目标之一是确保该地区的粮食安全,并形成旨在预防疾病的健康生活方式。据Rospotrebnadzor说,近年来,由于人口免疫缺陷,传染病在该地区广泛传播。一个人决定开发一种具有免疫调节特性的复杂膳食补充剂,并将其引入批量生产的可行性。一组烘焙产品是功能性产品的基础。本文介绍了在亚马洛-涅涅茨自治区以肉桂玫瑰(Fructus Rosae)和黑莓(黑莓,Empetrii nigri herba)为原料,添加植物原料的烘焙产品的研究结果。本研究的目的是证实以北极植物原料为基础的复合食品添加剂丰富了大众需求的烘焙产品的开发。制备工艺主要包括:原料分选;磨干精矿;在生物反应器中加热并包装。研究人员使用干燥形式的原料:肉桂玫瑰果- TU 10.89.19-036-14721358-2017;shiksha(黑莓)- TU 10.89.19-086-14721358-2017)。经感官和理化分析,产品符合现行食品法规要求。在安全性和功能特性方面的积极评估使植物原料中的添加剂获得功能性食品成分的资格- GOST R 54059-2010“功能性食品产品”。功能性食品配料。分类和一般要求:俄罗斯联邦国家标准”。烘焙产品本身是指富含功能性食品成分的产品——GOST R 55577-2013《专业功能性食品》。关于特色和有效性的资料:俄罗斯联邦国家标准”。
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引用次数: 1
Research of the Chemical Composition and Acute Toxicity of Wild Grasses Decoctions as a Prescription Component of Soft Drinks 野生草煎液作为软饮料配方成分的化学成分及急性毒性研究
Q Engineering Pub Date : 2022-06-21 DOI: 10.29141/2500-1922-2022-7-2-1
R. A. Fedorova, T. Kotova, Anastasia S. Valnyukova, Konstantin A. Khudyntsev, Yulia S. Fedorova, Olga Yu. Tikhonova
Excessive formation of free radicals in the human body leads to the biologically active compounds destruction, jeopardizing well-being, causing decrease in immunity and leading to premature aging. Inter alia, the use of soft drinks containing antioxidants (flavonoids, phenolic compounds and other organic substances) can reduce the harmful oxidative process impact on the body. A man used medicinal sage (Salvia officinalis), peppermint (Mentha piperita L.), sand immortelle (Helichrysum arenarium L.) as sources of biologically active compounds during the study. The research aims at studying the chemical composition and acute toxicity of decoctions from a mixture of medicinal sage, peppermint, sand immortelle, recommended in the non–alcoholic beverages production. The authors prepared three decoctions, differing in the quantitative content of herbs mixture; then determined an antioxidant activity and quantitative content of biologically active compounds in herbal decoctions. They used liquid chromatography-mass spectrometry to identify the phenolic composition; the flavonoids content was determined on a spectrophotometer SF-46, the quantitative active substances content of plant raw materials - on a chromatograph “Cvet Yauza-04”. A man studied acute toxicity on CD-I outbred mice (males and females) by injecting the maximum permissible volume of herbal decoctions into the stomach through a probe – 0.8 ml per 29 g, which was a dose of 5.0 g/kg of animal weight. The study results demonstrated that herbal decoctions are safe / low-toxic for mice. According to the research results, decoctions from herbs mixture of medicinal sage, peppermint and sand immortelle can be recommended as a ready-made drink in the capacity of flavonoids, phenolic compounds and other organic substances.
人体内自由基的过度形成会导致生物活性化合物的破坏,危害健康,导致免疫力下降,导致过早衰老。除其他外,使用含有抗氧化剂(类黄酮、酚类化合物和其他有机物质)的软饮料可以减少有害的氧化过程对身体的影响。一名男子在研究中使用药用鼠尾草(Salvia officinalis),薄荷(Mentha piperita L.),沙不朽草(Helichrysum arenarium L.)作为生物活性化合物的来源。本研究旨在研究在无酒精饮料生产中推荐使用的鼠尾草、薄荷、长白沙混合煎剂的化学成分和急性毒性。作者配制了三种煎剂,在混合药液的定量含量上存在差异;然后测定了中药煎剂的抗氧化活性和生物活性化合物的定量含量。他们用液相色谱-质谱法鉴定了酚类成分;用SF-46分光光度计测定黄酮类化合物含量,用Cvet Yauza-04色谱仪测定植物原料中活性物质含量。一个人对cd - 1型杂交小鼠(雄性和雌性)的急性毒性进行了研究,通过探针将最大允许体积的草药煎剂注射到胃中,每29克0.8毫升,剂量为5.0克/公斤动物体重。研究结果表明,中药煎剂对小鼠安全/低毒。根据研究结果,在黄酮类化合物、酚类化合物和其他有机物的含量方面,可以推荐鼠尾草、薄荷和永沙混合的煎剂作为现制饮料。
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引用次数: 1
Plant Antioxidants in the Oxidative Mayonnaise Spoilage Study 植物抗氧化剂在蛋黄酱氧化变质中的作用研究
Q Engineering Pub Date : 2022-06-21 DOI: 10.29141/2500-1922-2022-7-2-3
M. Shkolnikova, E. Rozhnov, Yulia Golubtsova, Elena E. Banshchikova, Elena A. Kovtun
The article presents the data systematization results on the plant antioxidants use in mayonnaise, that is a popular seasoning at home and in the food industry. The objective disadvantage of fat-containing products is the process of self-oxidation under the influence of a number of factors changing the organoleptic properties of mayonnaise, reducing nutritional value, safety and shelf life. Manufacturers solve the lipid oxidation problem of fat-containing products and the fat phase of mayonnaise by using officially registered antioxidants / oxidation inhibitors mainly of synthetic origin. Due to the increased demand for natural antioxidants caused by the growing market of food products with natural food additives, one of the promising approaches to the mayonnaise oxidation prevention is the use of isolated individual substances and plant extracts with the high antioxidant activity. The paper systematizes the examples of the plant antioxidants use in the mayonnaise composition described in modern scientific literature, reveals the features of their influence on the oxidative spoilage process and organoleptic indicators. A man indicated that plant extracts containing polyphenolic substances, vitamins, carotenoids, α-tocopherol and terpenes inhibit the mayonnaise oxidative spoilage process, acting as primary antioxidants slowing down the lipid oxidation onset, and secondary – reducing the oxidation rate. The research proved the plant antioxidants ability to suppress targets of oxidant activity – hydroxyl radical, superoxide, singlet oxygen lipid radical, lipid peroxides and metals of variable valence.
本文对蛋黄酱中植物抗氧化剂的应用进行了数据系统整理。含脂产品的客观缺点是在许多因素的影响下,自氧化过程改变了蛋黄酱的感官特性,降低了营养价值、安全性和保质期。制造商通过使用官方注册的主要合成来源的抗氧化剂/氧化抑制剂来解决含脂肪产品的脂质氧化问题和蛋黄酱的脂肪相。随着天然食品添加剂市场的不断增长,对天然抗氧化剂的需求不断增加,使用具有高抗氧化活性的分离个体物质和植物提取物是预防蛋黄酱氧化的一种很有前途的方法。本文系统介绍了现代科学文献中植物抗氧化剂在蛋黄酱成分中的应用实例,揭示了它们对氧化变质过程和感官指标的影响特点。有人指出,含有多酚类物质、维生素、类胡萝卜素、α-生育酚和萜烯的植物提取物可以抑制蛋黄酱的氧化变质过程,作为一级抗氧化剂,减缓脂质氧化的发生,并降低二级氧化速率。研究证实了植物抗氧化剂具有抑制氧化活性靶标-羟基自由基、超氧化物、单线态氧脂质自由基、脂质过氧化物和变价金属的能力。
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引用次数: 0
Biotechnological Aspects of the Functional Marmalade Development Using Whey for the Gastrointestinal Diseases Prevention 利用乳清开发功能性果酱预防胃肠道疾病的生物技术研究
Q Engineering Pub Date : 2022-06-21 DOI: 10.29141/2500-1922-2022-7-2-8
Maria A. Poluyanova, V. Popov
The article reveals the development processes and scientific substantiation of the marmalade of functional use, that is a functional product formulation with biologically active bifidogenic properties contributing to the prevention of the gastrointestinal tract diseases. The research relevance is due to the need to prevent the incidence of a new type of coronavirus COVID-19, that affects many systems of the human body, reduces immunity, while using drug therapy often leads to the natural intestinal microflora violation. To prevent the complications development, it is worth using food products capable of stopping destructive processes in the gastrointestinal mucosa during rehabilitation measures. The researchers developed the cranberry marmalade formulations with the probiotic culture introduction of a different mass fraction: sample 1 with probiotics in a free state, sample 2 with introduced immobilized probiotics; sample 3 with pasteurized whey enriched with a bacterial concentrate containing 0.001 % acidophilus bacillus. The control sample were the recipe of fruit and jelly marmalade. A man obtained a juice concentrate by pressing using an enzyme preparations composition of cellulolytic and pectolytic action. The researchers used the vacuum evaporation method to concentrate the juice to a dry matter content of 52-55 %; prepared the marmalade mass under laboratory conditions using traditional technology with a change in prescription components: probiotic culture introduction at the stage of swollen pectin into the cooled syrup at a temperature below 45 °C. The authors used mathematical modeling of formulations in the technological process to establish the ingredients optimal ratios; identified the rational introduction methods of acidophilus bacillus depending on the organoleptic and rheological product properties after a five-fold measurements repeatability.
本文揭示了功能性用途果酱的开发过程和科学依据,这是一种具有生物活性的双歧性特性,有助于预防胃肠道疾病的功能性产品配方。研究的相关性是由于需要预防新型冠状病毒COVID-19的发生,这种新型冠状病毒影响人体的许多系统,降低免疫力,同时使用药物治疗往往导致天然肠道微生物群的破坏。为了防止并发症的发展,在康复措施中使用能够阻止胃肠道粘膜破坏性过程的食品是值得的。研究人员开发了含有不同质量分数的益生菌培养的蔓越莓果酱配方:样品1含有游离状态的益生菌,样品2含有固定化的益生菌;样品3:巴氏灭菌乳清富含含有0.001%嗜酸杆菌的细菌浓缩物。对照样品为水果果冻果酱配方。一个人用一种具有纤维素分解和心肌分解作用的酶制剂压榨得到浓缩果汁。研究人员使用真空蒸发法将果汁浓缩到干物质含量为52- 55%;在实验室条件下,采用传统工艺制备果酱块,改变配方成分:在膨胀果胶阶段将益生菌培养物引入冷却糖浆中,温度低于45℃。在工艺过程中对配方进行数学建模,确定了各成分的最佳配比;通过5倍重复性实验,确定了嗜酸杆菌的合理引入方法。
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引用次数: 0
Technology Improvement of Minced Fish from Pond Fish and Quality Assessment of Culinary Products from It 塘鱼肉糜的工艺改进及食用产品质量评价
Q Engineering Pub Date : 2022-06-21 DOI: 10.29141/2500-1922-2022-7-2-7
N. Yartseva, N. Dolganova, I. Y. Aleksanian, A. Nugmanov
The fishery of the Caspian Sea basin plays an important role in providing the Southern Federal District population with food. Along with the coastal and marine fishery development due to the new area assimilation and underutilized facilities, the aquaculture production is a promising direction for the fishery development of the Southern Federal District. A man suggests to solve the efficient utilization problem of pond fish by developing relatively cheap, but high-quality public catering products, particularly various semi-finished products. When processing pond fish into minced fish, it is possible to obtain products of different quality classes differing in color, consistency, possible shelf life, and direction of use. Improving the minced fish production technology discovers new, more rational and modern ways of processing fish raw materials: processing into minced fish solves the production economic task, production of minced fish culinary products both natural and with additives enables expanding the fish products range on the Russian market. At the same time, a man solves the technological problem: since the pond fish has many small bones, when ground into minced fish, small bones either remain in the separator or are very finely minced; at the same time, the fish edible part yield reaches 40-60%, whereas when filleted is only 28-33%. As for the market, in recent years, consumer interest in food products that contain ingredients beneficial to human health has increased. The processing of pond fish into minced fish and the development of products based on it fall into the modern food market structure in general and the fish market in particular.
里海盆地的渔业在向南部联邦区人口提供食物方面发挥着重要作用。随着新区同化和未充分利用的沿海和海洋渔业的发展,水产养殖生产是南部联邦区渔业发展的一个有希望的方向。有人建议通过开发相对便宜但质量好的公共餐饮产品,特别是各种半成品,来解决塘鱼的高效利用问题。当将池塘鱼加工成碎鱼时,可能会得到不同质量等级的产品,在颜色、稠度、可能的保质期和使用方向上存在差异。改进鱼糜生产技术发现了新的、更合理和现代的鱼原料加工方式:加工成鱼糜解决了生产经济任务,生产天然和添加添加剂的鱼糜烹饪产品扩大了俄罗斯市场上的鱼产品范围。与此同时,一个人解决了技术问题:由于池塘鱼有很多小骨头,当磨成碎鱼时,小骨头要么留在分离器里,要么被剁得很细;同时,鱼的可食用部分产量可达40-60%,而鱼片产量仅为28-33%。就市场而言,近年来,消费者对含有有益人体健康成分的食品的兴趣有所增加。将塘鱼加工成鱼糜,并以此为基础开发产品,属于现代食品市场结构,特别是鱼类市场。
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引用次数: 0
Encapsulation Use for the Property Retention of the Vegetable Oils Blend 包封用于保持植物油混合物的特性
Q Engineering Pub Date : 2022-03-21 DOI: 10.29141/2500-1922-2022-7-1-5
N. Podgornova, A. Grunina
The balance of fatty acids obtained by the human body belonging to different groups (saturated, monounsaturated, polyunsaturated) and families (Omega-3, Omega-6) are the basis of their beneficial effect on metabolic processes. The article purpose is to substantiate the encapsulation use of vegetable oils blend balanced in fatty acid composition. A man developed the optimal composition of the vegetable oils blend by mathematical optimization methods using various software products. Based on the optimization results of the fatty acid composition, a man revealed a blend of three oils: rosehip, pumpkin and sea buckthorn. However, exposure to adverse conditions leads to the fact that vegetable oils undergo oxidative processes causing their spoilage and rancidity. To solve this problem, the researchers used the encapsulation technology to protect the easily oxidized ingredients of the product and control their release. To date, there are many encapsulation methods of biologically active substances. The authors analyzed information on the practical application and technological features of some encapsulation methods. They developed and tested a drip method with averaged for encapsulation of vegetable oils blend. This method enables to obtain microcapsulated products with high-tech properties serviceable for practical use.
人体获得的不同类别(饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸)和家族(Omega-3、Omega-6)脂肪酸的平衡是它们对代谢过程有益作用的基础。本文的目的是证实脂肪酸组成平衡的植物油混合油的胶囊化使用。一个人利用各种软件产品通过数学优化方法开发了植物油混合物的最佳组成。根据脂肪酸组成的优化结果,一位男士揭示了三种油的混合物:玫瑰果、南瓜和沙棘。然而,暴露在不利的条件下会导致植物油经历氧化过程,导致其腐败和酸败。为了解决这个问题,研究人员使用了封装技术来保护产品中容易氧化的成分,并控制其释放。迄今为止,生物活性物质的包封方法有很多。分析了几种封装方法的实际应用和工艺特点。他们开发并测试了一种平均滴注方法来封装植物油混合物。这种方法能够获得具有高科技性能的微胶囊产品,可用于实际应用。
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引用次数: 0
Kinetic Regularities Research of the Convective Concentration Process of a Hexane-Paraffinic Composition for the Food Paraffin Production 食品石蜡生产中正己烷-石蜡组合物对流浓缩过程动力学规律研究
Q Engineering Pub Date : 2022-03-21 DOI: 10.29141/2500-1922-2022-7-1-1
M. Maryisheva, I. Y. Aleksanian, A. Nugmanov, L. Titova
Film structures, used for the production of biodegradable packaging materials, are hydrophilic in nature. Their paraffinization reduced the influence of external factors, primarily moisture, for them, i.e. applying a thin layer of molten food paraffin to the film surface for subsequent protection of packaged food products from moisture and sunlight. While producing the moisture-repellent compositions, a man pays much attention to the quality of the final food paraffin. One of its important safety indicators is the absence of carcinogenic aromatic hydrocarbons in it. The kinetic regularities study of the convective concentration processes of a technical paraffin solution in n-hexane is necessary to analyze their mechanism and speed, depending on the operating parameters, making possible to determine the rational duration of these procedures and the specific yield of the resulting product. To rationalize the convective concentration of a hexane solution, it is necessary to select such operating parameters enabling not only to remove hexane with toxic components dissolved in it from the study object in a relatively simple apparatus, but also to significantly reduce the time for this process. The study aim was to determine the rational modes of hexane-paraffin mixture concentration, as an object for the food paraffin production with convective energy supply. The concentration curve nature, although it is typical for the interacting materials, in particular n-hexane and paraffin, has a certain specificity, determined by the physicochemical parameters of the research object, which is a true solution included various toxic substances. The specific features of the study object are to be reflected not only on the solution concentration mechanism, but also on the temperature fields evolution in it and, as a result, determine the original approaches to modeling the process of removing the solvent together with toxic substances, particularly, benz-α-pyrene.
用于生产可生物降解包装材料的薄膜结构本质上是亲水的。他们的石蜡化减少了外界因素的影响,主要是水分,对他们来说,即在薄膜表面涂上一层薄薄的熔融食品石蜡,以保护包装食品免受水分和阳光的影响。在生产防潮成分时,一个人非常注意最终食品石蜡的质量。其重要的安全指标之一是不含致癌性芳烃。对正己烷中工艺石蜡溶液的对流浓缩过程进行动力学规律研究,分析其机理和速度,根据不同的操作参数,确定这些过程的合理时间和产物的比产率。为了使正己烷溶液的对流浓度合理化,有必要选择这样的操作参数,既可以在相对简单的设备中从研究对象中去除含有有毒成分的正己烷,又可以大大减少该过程的时间。研究的目的是确定正己烷-石蜡混合物的合理浓度模式,为对流供能食品石蜡生产提供研究对象。浓度曲线性质虽然是典型的相互作用物质,特别是正己烷和石蜡,但具有一定的专一性,这是由研究对象的理化参数决定的,是包含各种有毒物质的真实溶液。研究对象的具体特征不仅体现在溶液浓度机理上,而且体现在其中的温度场演变上,从而确定了溶剂与有毒物质(特别是苯并α-芘)一起去除过程的原始建模方法。
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引用次数: 0
Bifidogenic Properties of Barley Seedling Extracts 大麦幼苗提取物的双歧性研究
Q Engineering Pub Date : 2022-03-21 DOI: 10.29141/2500-1922-2022-7-1-7
Y. Zakharova, T. Kotova, O. Soboleva, A. Sukhikh, V. Le
The study subject is the bifidobacteria reproduction kinetics in the presence of barley seed extracts. A man run the research in order to study the influence of the extract nature of barley seedlings microgreens with different preparation variants for sowing on representatives of the genus Bifidobacterium in vitro. There were two strains used: Bifidobacterium bifidum 791 and Bifidobacterium bifidum 20. Dry seeds were exposed to microwave radiation with a power of 0.42 kW and 0.70 kW before sowing, the magnetron frequency was 2.45 GHz, the exposure was 11 sec. Then a man sprouted the grains for seven days. The researchers determined the sugar composition in the extracts by the Shimadzu LC-20 Prominence chromatograph. monitored the bacterial growth kinetics by the spectrophotometer SF-2000 (Experimental Design Bureau “Spectrum”, Russia), recording the optical density of the cuvette contents hourly for 20 hours. There was the reproduction stimulation of B. bifidum 791 and B. bifidum 20 in the exponential phase with an extract from barley seedlings treated with 0.42 kW microwave beams. Moreover, there were stable optical density indicators of B. bifidum 791 in the stationary phase, mainly when adding barley extract with maximum processing power. According to the chromatography data, the trietol content, a powerful antioxidant; and galactose, a bifidogenic prebiotic, increases in seed extracts with such preparation options. A man can use the barley extracts treated with microwave rays in biotechnological processes to reduce the adaptation time of bifidobacteria in periodic culture and to form a high population density in the stationary growth phase.
研究对象是大麦种子提取物对双歧杆菌繁殖动力学的影响。一名男子进行了这项研究,以研究不同制剂变体播种的大麦幼苗微芽提取物性质对双歧杆菌属代表菌的体外影响。采用两歧双歧杆菌791和两歧双歧杆菌20两种菌株。干燥的种子在播种前分别暴露在功率为0.42 kW和0.70 kW的微波辐射下,磁控管频率为2.45 GHz,暴露时间为11秒,然后由一名男子连续7天发芽。研究人员用岛津LC-20显像色谱仪测定了提取物中的糖成分。用SF-2000分光光度计(俄罗斯“光谱”实验设计局)监测细菌生长动力学,每小时记录培养皿内容物的光密度,持续20小时。在0.42 kW微波波束处理下,大麦苗提取物对两歧双歧杆菌791和二歧双歧杆菌20在指数期的繁殖有刺激作用。两歧双歧杆菌791在固定阶段的光密度指标较为稳定,主要表现在添加处理能力最大的大麦提取物时。根据色谱数据,三萜类醇含量高,抗氧化剂强;而半乳糖,一种双歧益生元,在这样的制备选择中会增加种子提取物的含量。利用微波处理的大麦提取物进行生物工艺处理,可以缩短双歧杆菌周期性培养的适应时间,在固定生长阶段形成较高的种群密度。
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引用次数: 1
Efficiency Evaluation of the Carotenoids Extraction from Brown Algae S. Miyabei and Their Stability during Storage 米氏褐藻类胡萝卜素提取效率及贮藏稳定性评价
Q Engineering Pub Date : 2022-03-21 DOI: 10.29141/2500-1922-2022-7-1-6
A. Tabakaev, O. Tabakaeva
The article concerns the results of evaluating the carotenoid extraction efficiency from brown algae S. miyabei and their stability during storage. The anatomical parts of the brown algae S. miyabei – thallom and phylloids – have a high content of carotenoids; hence there is a conclusion about the prospects of their use as a carotenoid source. Among the carotenoids of the brown algae S. miyabei, xanthophylls prevail over carotenes (exceeding – 7.4-10.6 times). The researchers have experimentally proved that the extraction efficiency depends on the type of vegetable oil or oil mixture used individually. So, soybean oil and mixtures based on it are the best carotenoid extractants from brown algae S. miyabei compared to sunflower, olive, corn oils and mixtures based on them. Carotenoids in extracts from algae S. miyabei are mainly xanthophylls, which content exceeds the carotenes one by 4.4-5.7 times in extracts based on individually used vegetable oils and by 3.2–6.1 times in extracts based on their mixtures. A man proved sufficiently high stability of xanthophylls during storage. The storage for 6 months leads to a decrease in their content by 7–14 % when using individual oils as extractants and by 6–20 % when using oil mixtures. During storage, there was a significant decrease in the carotenes content – up to 50–75 % of the original content in the extract. The maximum decrease in carotene content was in extracts based on sunflower, corn oils and a mixture of olive and sunflower oils in a ratio of 1:1.
本文对宫氏褐藻中类胡萝卜素的提取效率及其贮藏稳定性进行了评价。褐藻的解剖部位——叶状体和叶状体——类胡萝卜素含量高;因此,对其作为类胡萝卜素来源的前景有一个结论。在褐藻类胡萝卜素中,叶黄素含量高于胡萝卜素(超过- 7.4-10.6倍)。研究人员通过实验证明,提取效率取决于单独使用的植物油或油混合物的类型。因此,与葵花籽油、橄榄油、玉米油及其混合物相比,大豆油及其混合物是最好的类胡萝卜素萃取剂。紫杉藻提取物中的类胡萝卜素主要为叶黄素,单独使用植物油提取物的类胡萝卜素含量是叶黄素的4.4 ~ 5.7倍,混合使用植物油提取物的类胡萝卜素含量是叶黄素的3.2 ~ 6.1倍。在贮存过程中,证明了叶黄素具有很高的稳定性。当使用单独的油作为萃取剂时,6个月的储存导致其含量减少7 - 14%,当使用混合油时,减少6 - 20%。在储存过程中,胡萝卜素的含量显著下降,达到原始提取物含量的50 - 75%。以葵花籽油、玉米油以及橄榄油和葵花籽油的混合物为原料,按1:1的比例提取的提取物中,胡萝卜素含量下降幅度最大。
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引用次数: 1
Research of the Photostimulation Effect on the Lactobacillus Lactis Culture Productivity and Resulting Nisin Activity 光刺激对乳酸菌培养产量及乳杆菌素活性影响的研究
Q Engineering Pub Date : 2022-03-21 DOI: 10.29141/2500-1922-2022-7-1-8
S. Tikhonov, N. Tikhonova, I. G. Pestova, M. S. Tikhonova
The article concerns the study results of the photostimulation influence of the Lactobacillus lactis inoculum on the cultural effervescence and obtained nisin activity. The researchers used frozen Lactobacillus lactis inoculum to produce nisin. They activated the culture in a 5.0 ml sterile MRS tube kept in an incubator at 37 °C for 48 hours. Before incubation, Lactobacillus lactis culture was treated with blue spectrum light with a wavelength of 430–470 nm and a luminous flux intensity of 35 MW/cm2 for 1 hour (experimental group). Bacterial samples of the control group were not treated with light. A man used the MRS medium for fermentation. To determine the culture titer, the authors run sowing on the MRS medium. The researchers defined an oxidative stress influence on the Lactobacillus lactis stability by cultivation on an agar medium using 5 mmol of hydrogen peroxide. To identify the titer and resistance to oxidative stress before sowing, the cultures were treated with blue light with the specified characteristics and processing time. It enables to increase the nisin activity after 24 hours of bacterial cultivation compared to the control group by 19.3%, or by 558 IU/ml. The researchers proved that the titer of Lactobacillus lactis culture against the background of preliminary irradiation with blue spectrum light before cultivation is 56.3% higher than the samples of the control group. Treatment of Lactobacillus lactis culture with blue spectrum light increases its viability under oxidative stress by 75%.
本文报道了乳杆菌接种物的光刺激对培养泡腾率和所得乳酸链球菌素活性影响的研究结果。研究人员使用冷冻乳酸杆菌接种物来生产乳酸链球菌素。他们在5.0 ml无菌MRS管中激活培养物,在37°C的培养箱中保存48小时。孵育前,用波长430 ~ 470 nm、光通量强度35 MW/cm2的蓝光处理乳酸乳杆菌培养物1小时(实验组)。对照组的细菌样本不进行光照处理。一个人用MRS培养基进行发酵。为了确定培养滴度,作者在MRS培养基上进行了播种。研究人员通过在琼脂培养基上用5mmol过氧化氢培养乳酸乳杆菌,确定了氧化应激对乳酸乳杆菌稳定性的影响。为了在播种前鉴定其滴度和抗氧化能力,对培养物进行了具有特定特性和处理时间的蓝光处理。细菌培养24小时后,与对照组相比,可使nisin活性提高19.3%,或提高558 IU/ml。研究人员证明,在培养前用蓝色光谱光进行初步照射的背景下,乳酸乳杆菌培养物的滴度比对照组样品高56.3%。蓝光处理乳酸乳杆菌培养物可使其在氧化胁迫下的生存能力提高75%。
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引用次数: 0
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Agro Food Industry Hi-tech
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