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Enzyme Composition Modeling and Its Application in the Baking Cryotechnology 酶组成建模及其在烘焙低温技术中的应用
Q Engineering Pub Date : 2023-03-22 DOI: 10.29141/2500-1922-2023-8-1-1
S. Kitaevskaya
Cryotechnologies are widely used in the production of bakery products, including dough. The study aim is to optimize the enzyme composition and evaluate its application effectiveness in the technology of rye-wheat bread based on frozen dough. To solve nonlinear problems and the fractional factor experiment method a man applied algorithms of the generalized reduced gradient method. As a result of data analysis using MS Office Excel, the maximum possible concentrations of the enzyme complex components are, wt. %: “Pentopan 500 BG” – 12.0, “Novamil 1500 MG” – 5.0 and “Neutrase 1.5 MG” – 2.0; the optimal ratio is 6:2.5:1. The authors run the recipe optimization of rye-wheat bread based on frozen dough using Statgraphics 18.1 and constructing mathematical models based on multifactorial analysis. The variables were the dosages of the enzyme composition 4–10 % by weight of flour and dry starter culture of lactic acid bacteria 0.05–0.15 % by weight of flour; the required functions were porosity and specific volume of rye-wheat bread. As the mathematical data processing result, a man received regression dependence equations of rye-wheat bread porosity and specific volume on the components concentrations; optimal dry starter concentrations and enzyme composition – 0.12 and 7.5 % by weight of flour, respectively. The thesis provides physicochemical, organoleptic and structural-mechanical characteristics of rye-wheat bread developed on the cryotechnology basis after long-term low-temperature storage of test dough at a temperature of minus 18–20 ° C for 150 days, confirming the enzyme composition effectiveness.
冷冻技术广泛应用于烘焙产品的生产,包括面团。本研究的目的是优化酶的组成,并评价其在冷冻面团黑麦面包工艺中的应用效果。为了解决非线性问题和分数因子实验方法,应用了广义约简梯度法的算法。利用MS Office Excel进行数据分析,酶复合物组分的最大可能浓度为:wt. %:“Pentopan 500 BG”- 12.0,“Novamil 1500 MG”- 5.0和“Neutrase 1.5 MG”- 2.0;最佳比例为6:2.5:1。利用Statgraphics 18.1软件,构建基于多因子分析的数学模型,对冷冻面团黑麦面包的配方进行优化。变量为面粉重量4 - 10%的酵素组合物用量和乳酸菌重量0.05 - 0.15%的干发酵剂用量;所需的功能是黑麦面包的孔隙率和比体积。作为数学数据处理的结果,得到了黑麦面包孔隙率和比容对各组分浓度的回归依赖方程;最佳干发酵剂浓度和酶组成分别为面粉重量的0.12%和7.5%。本文通过对实验面团在零下18-20℃的低温下长期保存150天,在冷冻技术的基础上制作的黑麦面包的理化、感官和结构力学特征,证实了酶组成的有效性。
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引用次数: 0
Possibility Assessment of Using Dihydroquercetin and Pectin in the Oil Pastes Composition to Increase Their Nutritional Value 双氢槲皮素和果胶在油膏成分中提高其营养价值的可能性评价
Q Engineering Pub Date : 2023-03-22 DOI: 10.29141/2500-1922-2023-8-1-8
M. Zyablitseva
The fats proportion in the diet of a modern person should not exceed 30 % of the total energy value of the diet. Butter paste is a product of buttermaking with a fat content of 39 to 49 %. The study aims at evaluating the possibility of dehydroquercetin and pectin use in the butter pastes composition to increase their nutritional value. The researchers devel oped butterpaste composition with dihydroquercetin “Lavitol” and pectin. There are the organoleptic, physico-chemical and microbiological parameters of the butter paste with pectin and dihydroquercetin – the paste has a dense, plastic, homogeneous consistency and a pronounced creamy taste. The maximum value of the thermal stability coefficient (0.93) was in samples with a dosage of 1.5 % pectin. The thermal stability coefficients of the control sample and the sample with dihydroquercetin were 21.1 and 3.88 % lower than in the experimental sample with dihydroquercetin and pectin. The minimum ther mal stability coefficient was in samples without pectin (0.73). The mass fraction of fat in the butter paste was 44 %. The authors revealed an increase in thermal stability during the dihydroquercetin introduction, a decrease in the milk plasma acidity and the number of mesophilic aerobic and facultative anaerobic microorganisms in butter paste samples with dihydroquercetin by 29.5 % on the 1st day and by 38.7 % on the 10th day of storage. A man developed butter paste formulation with pectin and dihydroquercetin. The butter paste production according to this recipe is possible in the conditions of a dairy processing enterprise of any capacity, with organized butter manufacture. The product targets the systematic use by the population of all age groups.
现代人饮食中的脂肪比例不应超过饮食总能量值的30%。黄油糊是黄油制作的产品,脂肪含量为39%至49%。本研究旨在评价脱氢槲皮素和果胶在牛油糊成分中使用以提高其营养价值的可能性。研究人员开发了一种由二氢槲皮素和果胶组成的黄油糊。这是含有果胶和二氢槲皮素的黄油糊的感官、物理化学和微生物参数——这种糊具有致密、塑料、均匀的稠度和明显的奶油味。果胶用量为1.5%时,其热稳定性系数最大,为0.93。对照样品和添加双氢槲皮素的样品的热稳定系数分别比添加双氢槲皮素和果胶的实验样品低21.1%和3.88%。无果胶样品的热稳定性系数最小(0.73)。黄油膏中脂肪的质量分数为44%。结果表明,添加双氢槲皮素后,牛油膏样品的热稳定性提高,血浆酸度下降,中温好氧和兼性厌氧微生物数量在第1天下降29.5%,在第10天下降38.7%。有人用果胶和二氢槲皮素研制了黄油膏配方。在有组织的黄油生产条件下,任何能力的乳制品加工企业都可以根据该配方生产黄油膏。该产品针对所有年龄段人群的系统使用。
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引用次数: 0
Small γ-Radiation Doses Impact in the Conditions of Controlled Micropenological Octores of Spring Barley 小γ辐射剂量对春大麦微生育期调控条件的影响
Q Engineering Pub Date : 2023-03-22 DOI: 10.29141/2500-1922-2023-8-1-2
R. Timakova, R. Iliukhin
Barley, characterized by early ripening, cold and drought resistance, is an important agricultural grain crop. Ionizing radiation use in the pre-sowing seed treatment technology is promising in order to control pathogens and increase the grain crops yield. The study aims at developing a microphenological sprouting phases scale of spring barley variety in Remembrance of Vyacheslav Chepelev treated with small doses of gamma radiation under controlled ontogeny conditions. The authors gave the concepts interpretation of “sprouted grain” and “grain sprouts”. To remove the dormant state of the original seeds (grains) of spring barley variety in Remembrance of Vyacheslav Chepelev harvested in 2021 and zoned in the Sverdlovsk region, a man treated it with small doses of γ-radiation ionizing (5, 10, 15 and 20 Gy) by a radiation source 60Co. The researchers developed a microphenological sprouting phases scale of spring barley grains with the dry grain phase and the micrograin phase introduced; and classification levels differentiated. A man revealed that the change in grain moisture and grain germ in all microphenophases was comparable with the results obtained during barley sprouting. The most intensive water absorption occurs at the swelling phase with an increase in grain moisture by 2.7 times and in the embryo by 9.3 times. Spring barley grains radiostimulation has an impact on the grain sprouting due to increased germination energy. The authors determined that after 5 days there was a maximum increase in the germination capacity to (92 ± 2) % and (94 ± 2) % in barley grains samples treated with doses of 5 and 10 Gy, respectively, with lower values when processing grains with doses of 15 Gy and 20 Gy. A man ascertained inhibitory effect of a radiation dose of 20 Gy. Despite a higher germination energy index of 75 % in barley grains treated with a dose of 20 Gy, and unlike untreated grains – 74 %, the germination ability in radiation-treated grains was 4 % lower. The germination rate of the first seed is 3 days on average.
大麦是一种重要的农业粮食作物,具有早熟、耐寒、抗旱等特点。将电离辐射应用于播前种子处理技术在防治病原菌和提高粮食作物产量方面具有广阔的应用前景。研究了在可控的个体发育条件下,小剂量伽玛辐射处理的“切别列夫纪念”春大麦品种发芽期的微物候尺度。对“发芽粒”和“谷芽”的概念进行了解释。为了消除在斯维尔德洛夫斯克地区于2021年收获的春大麦品种的原始种子(谷物)的休眠状态,一名男子用辐射源60Co对其进行了小剂量的γ辐射电离(5,10,15和20 Gy)处理。引入干粒期和微粒期,建立了春大麦籽粒萌发的微物候期量表;分类水平有差异。一个人透露,在所有微物候期谷物水分和谷物胚芽的变化与大麦发芽期间获得的结果相当。溶胀期吸水最剧烈,籽粒水分增加2.7倍,胚期增加9.3倍。春大麦籽粒辐照后萌发能量增加,对籽粒萌发有影响。实验结果表明,5 Gy和10 Gy处理5天后,大麦种子萌发率最大增幅分别为(92±2)%和(94±2)%,15 Gy和20 Gy处理5天后萌发率较低。一名男子确定了20戈瑞辐射剂量的抑制作用。尽管20 Gy辐照处理的大麦籽粒萌发能指数高达75%,高于未处理的74%,但辐照处理的萌发能力却低4%。第一粒种子的发芽率平均为3天。
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引用次数: 0
Technological Properties Research of Biocomposites Based on the Natural Decomposable Compounds Reinforced with Buckwheat Husk 荞麦壳增强天然可分解化合物生物复合材料工艺性能研究
Q Engineering Pub Date : 2023-03-22 DOI: 10.29141/2500-1922-2023-8-1-10
Aleksandr Semukhin, N. Zavorokhina, E. Pastushkova
The need to process packaging materials based on petrochemical polymers raises the question of the material for packaging production, while the raw materials for packaging should be multi-tonnage, biodegradable, cost-effective. The buckwheat husk containing up to 50 % fiber, 3–4 % crude protein, 4–5 % fat, 0.2–0.3 % sugars, 9–10% ash, tannins, which is able to inhibit metal corrosion is of a particular interest for the research. Buck wheat husk has a high bulk density – about 145 kg/m3. It is hydrophilic and is able to swell increased in volume by up to 25 %. The study aims at developing composite systems based on natural decomposable compounds reinforced with buckwheat husks. The researchers studied gelatin, sodium alginate, xanthan and guar gum, glycerin, potato starch as plasti cizer compounds. They determined that the buckwheat husk surface under the microscope was porous with many cracks, which explains its hydrophilic properties. The thesis presents characteristics of biocomposites and films from them after drying. A man revealed that the glycerin use did not require water introduction, still the biocomposite had a crumbly structure and low water absorption capacity. Biocomposites based on sodium alginate had optimal characteristics: they were plastic, formed a strong film, were well applicable to the surface, had a water absorption of 65.33 and 71.08 %, respectively. The work presented physicomechanical parameters of biocomposite films.
需要加工基于石化聚合物的包装材料,这就提出了包装生产的材料问题,而包装的原材料应该是多吨位的、可生物降解的、高性价比的。荞麦皮含有高达50%的纤维,3 - 4%的粗蛋白质,4 - 5%的脂肪,0.2 - 0.3%的糖,9-10%的灰分,单宁,能够抑制金属腐蚀,是研究的一个特别感兴趣的地方。荞麦皮有很高的堆积密度-约145公斤/立方米。它是亲水的,能够膨胀体积增加25%。该研究旨在开发基于天然可分解化合物与荞麦壳增强的复合系统。研究人员研究了明胶、海藻酸钠、黄原胶和瓜尔胶、甘油、土豆淀粉作为增塑剂化合物。他们在显微镜下确定荞麦壳表面多孔,有许多裂缝,这解释了它的亲水性。本文介绍了生物复合材料的特性及其干燥后的薄膜。一名男子透露,甘油的使用不需要水的引入,但生物复合材料有一个易碎的结构和低吸水能力。海藻酸钠基生物复合材料具有良好的可塑性、膜性强、表面亲和性好、吸水率分别为65.33%和71.08%。研究了生物复合膜的物理力学参数。
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引用次数: 0
Organoleptic Quality Characteristics Research of Sauces from Secondary Aquatic Biological Resources 次生水生生物资源酱料的感官品质特性研究
Q Engineering Pub Date : 2023-03-22 DOI: 10.29141/2500-1922-2023-8-1-5
Olesia Iakubova, A. Bekesheva, Mariya Mukhanova
Currently, the use of concentrate sauces and semi-finished products based on broth from natural raw materials is relevant. When processing fish, it is rational to redirect the fish bones and scales to the sauces manufacture, since they have a high content of flavoring substances. The study aim is to develop descriptive organoleptic quality cha racteristics of sauce from secondary aquatic biological resources produced on the basis of silver carp bones and scales and carapace-containing raw materials of crawfish using modern biotechnological techniques. The recipe composition of the sauce includes fish bones and scales, carapace-containing raw materials of crawfish, the enzyme preparation “Alkalase 2.4 L FG”, carrots, onions, celery, bay leaf, salt, black pepper, guar gum. The re searchers identified the most significant identification sensory signs for the sauce, and built a descriptors panel; developed a descriptive scoring scale for evaluating organolep tic quality indicators of sauce from secondary aquatic biological resources; gave possible inconsistencies and a regulated reduction in the maximum organoleptic evaluation score of the sauce. A man run a qualimetric study of experimental samples considering the coef ficients of quality indicators significance; developed a reference sensory profile of sauce from secondary aquatic biological resources using the descriptor-profile method of tast ing analysis. The authors substantiated the sensory characteristics interrelation of the de veloped sauce with the chemical composition of the feedstock and production technology, with the glutamic and aspartic acids content in the fish scales of silver carp and the natural red pigment content in the shell of crawfish – astaxanthin. The obtained developments enable to run the organoleptic evaluation and quality control process of sauce products from secondary aquatic biological resources reliably and objectively, directly at the food industry enterprises.
目前,使用浓缩酱汁和基于天然原料的肉汤的半成品是相关的。在加工鱼类时,合理的做法是将鱼骨和鱼鳞转移到酱料制造中,因为它们含有高含量的调味物质。本研究的目的是利用现代生物技术,以鲢鱼骨鳞和小龙虾含壳原料为原料,生产二级水生生物资源,开发具有描述性的酱料感官品质特征。该酱料的配方成分包括鱼骨、鱼鳞、小龙虾含壳原料、酶制剂“碱酶2.4 L FG”、胡萝卜、洋葱、芹菜、月桂叶、盐、黑胡椒、瓜尔胶。研究人员确定了酱汁最重要的识别感官标志,并建立了一个描述符面板;开发了用于评价次生水生生物资源酱油有机品质指标的描述性评分量表;给出了可能的不一致和酱油的最大感官评价分数的调节降低。考虑到质量指标的显著性系数,对实验样本进行了定性研究;利用味觉分析的描述-剖面法建立了二级水生生物资源酱油的参考感官剖面。研制的酱料的感官特性与原料的化学成分和生产工艺、鲢鱼鱼鳞中的谷氨酸和天冬氨酸含量、小龙虾壳中的天然红色素虾青素含量有密切的关系。本研究成果可直接面向食品工业企业,可靠、客观地对次生水生生物资源酱油产品进行感官评价和质量控制。
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引用次数: 0
Grain Millgrist Ratio Influence on the Grain-Forming Ability and Triticale-Rye Flour Yield 粒比对成粒能力和黑麦-小黑麦面粉产量的影响
Q Engineering Pub Date : 2022-12-21 DOI: 10.29141/2500-1922-2022-7-4-6
R. Kandrokov, Kerim Bekshokov
The research revealed the study results of the influence of the different millgrist ratios of triticale grain and rye on the triticale-rye intermediate grinding products and triticale-rye flour yield. A man grinds the initial samples of triticale grains on laboratory grinding mills (MLP-4) with threaded (for break systems) and microroughened rolls (for reduction systems). The mechanical and kinematic parameters of the MLP-4 mill threaded rolls are as follows: the flute layout is back to back, the fast-rotating roll speed is 5 m/s, the differential unit is 1.5, the number of flutes per 1 cm is 8, the flutes slope is 7 %. A man revealed that among the presented samples the control triticale grain demonstrated the highest intermediate ground materials yield, which amounted to 87.9 %, including 14.2 % of triticale flour; while the control rye grain – the smallest intermediate ground materials yield, accounted for 81.9 %, including 22.4 % rye flour. The study showed that when 50 % of rye grain was added to the triticale-rye grain millgrist, the intermediate ground materials yield in the cereal dusting products form was 83.1 %, including 19.1% of triticale-rye flour; when 40 % of rye grain was added to the triticale-rye grain millgrist, the intermediate ground materials yield in the сereal dusting products form was 85.0 %, including 17.3 % of triticale-rye flour; when 30 % of rye grain was added to the triticale-rye grain millgrist, the intermediate ground materials yield in the сereal dusting products form amounted to 87.9 %, including 14.2 % of triticale-rye flour. A man found that when grinding the initial control triticale grain, the triticale flour yield was 77.9 %, when grinding the initial control rye grain, the triticale flour yield flour was 70.3 %, which is 6.9 % less than from triticale grain. When introducing 50 % of rye grain to the triticale-rye grain millgrist, the triticale-rye flour yield was 72.9 %; when introducing 40 % of rye grain to the triticale-rye grain millgrist, the triticale-rye flour yield was 72.9 %, when introducing 30 % of rye grain to the triticale-rye grain millgrist, the triticale-rye flour yield was 77.1 %. The authors connected the dots between the triticale-rye flour yield and the rye grains content in the millgrist. At the same time, they found that the rye grain introduction to the triticale-rye millgrist led to a decrease in the triticale-rye flour yield.
本研究揭示了小黑麦与黑麦不同粉磨比对小黑麦-黑麦中间粉磨产品及小黑麦-黑麦面粉产量影响的研究结果。一个人在实验室研磨机(MLP-4)上用螺纹(用于破碎系统)和微粗化辊(用于还原系统)研磨小黑麦颗粒的初始样品。MLP-4型轧机螺纹辊的机械和运动学参数为:横槽布置为背对背,快转辊速度为5m /s,差速单位为1.5,每1厘米纵槽数为8,纵槽坡度为7%。一名男子透露,在提交的样品中,对照小黑麦籽粒显示出最高的中间地面材料产量,达87.9%,其中小黑麦粉占14.2%;而中间磨粒产量最小的对照黑麦占81.9%,其中黑麦粉占22.4%。研究表明,当黑麦-黑麦混合粉中添加50%的黑麦粒时,谷物粉产品形态的中间磨粒产量为83.1%,其中黑麦-黑麦粉产量为19.1%;黑麦-黑麦磨粉机中添加40%黑麦粒时,粗粉产品形态中间磨粒产量为85.0%,其中黑麦-黑麦粉产量为17.3%;黑麦-黑麦磨粉机中添加30%黑麦粒时,粗粉产品中中间磨粒产量达87.9%,其中黑麦-黑麦粉产量为14.2%。一个人发现,研磨初始对照小黑麦粒时,小黑麦粉得率为77.9%,研磨初始对照黑麦粒时,小黑麦粉得率为70.3%,比从小黑麦粒中少6.9%。在黑麦-黑麦粉磨机中引入50%黑麦粒时,黑麦-黑麦粉产量为72.9%;黑麦-黑麦混合磨时,黑麦-黑麦产量为72.9%;黑麦-黑麦混合磨时,黑麦-黑麦混合磨时,黑麦-黑麦混合磨产量为77.1%。作者将黑麦-黑麦面粉产量与黑麦颗粒含量之间的点联系起来。同时,他们发现黑麦谷物引入小黑麦-黑麦磨坊导致小黑麦-黑麦面粉产量下降。
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引用次数: 0
Express Assessment Method for Color Characteristics of Cocoa-Containing Food Systems 含可可食品体系颜色特性的快速评价方法
Q Engineering Pub Date : 2022-12-21 DOI: 10.29141/2500-1922-2022-7-4-5
E. Rozhnov, M. Shkolnikova, E. Kadritskaya, A. Arisov
In the contemporary context, climatic conditions in cocoa bean-producing countries and disrupted logistics chains due to the coronavirus pandemic complicate stable cocoa products supply. The study aimed at developing an express assessment method for color characteristics of cocoa-containing food systems where cocoa powder replaced with brown pigment melanin obtained from buckwheat husks, partially. A man prepared five confectionery glaze prototypes: one based on non-alkalized cocoa powder and four samples containing 3; 5; 7 and 10 % melanin powder as a substitute for cocoa powder. The confectionery glaze based on alkalized cocoa powder served as a comparison sample. To study the glaze color characteristics, the researchers covered white plates with samples, enabled the glaze to harden, and then, took images of the glaze surface using a digital camera in a white box at a color temperature of 6000 K; processed images using image analysis software that provided color coordinates determination of any point of the uploaded image in the RGB model. Then, a man determined the color coordinates in trichromatic XYZ coordinates using a color converter. The results revealed that the confectionery glaze prototype containing 15% non-alkalized cocoa powder and 10% melanin was close to the control sample in terms of color characteristics. The suggested approach advantages are the expensive equipment absence, simplicity and data processing accessibility. The method enables running an objective color assessment of a cocoa-containing product, comparing product samples with each other, and defining color differences. The research results offer the challenges for the color scales development for cocoa-containing foods quality assessment.
在当前背景下,可可豆生产国的气候条件和冠状病毒大流行导致的物流链中断,使可可豆产品的稳定供应复杂化。本研究旨在开发一种用部分从荞麦壳中获得的棕色色素黑色素代替可可粉的含可可食品体系颜色特性的快速评估方法。一名男子准备了五种糖果釉原型:一种是基于非碱化可可粉的,四种是含有3的样品;5;7%和10%的黑色素粉作为可可粉的替代品。以碱化可可粉为基础的糖果釉料作为比较样品。为了研究釉的颜色特征,研究人员在白色的盘子上覆盖样品,使釉变硬,然后在6000 K的色温下,用数码相机在白色的盒子里拍摄釉表面的图像;使用图像分析软件处理图像,该软件提供了在RGB模型中上传图像的任何点的颜色坐标确定。然后,一个人使用颜色转换器确定三色XYZ坐标中的颜色坐标。结果表明,含有15%非碱化可可粉和10%黑色素的糖果釉原型在颜色特征上与对照样品接近。该方法的优点是没有昂贵的设备,操作简单,数据处理方便。该方法能够对含有可可的产品进行客观的颜色评估,相互比较产品样品,并确定颜色差异。研究结果为含可可食品质量评价的色标开发提出了挑战。
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引用次数: 0
Individual Peptides Extraction and Characteristics of Cow Colostrum Trypsin Hydrolysate with Biological Activity Assessment 牛初乳胰蛋白酶水解产物的单肽提取、特性及生物活性评价
Q Engineering Pub Date : 2022-12-21 DOI: 10.29141/2500-1922-2022-7-4-10
S. Tikhonov, N. Tikhonova, Angelika Popovskikh, M. Tikhonova
The research concerned the trypsin peptides analysis of colostrum hydrolyzate. The authors isolated seven individual peptides: TT1, TT2, TT3, T(1), T1.1, T1.2, mpT. They determined the molecular weight of the peptides and the amino acid sequence in the peptide chain by mass spectrometry; peptides – using the international database Protein NCBI. The mpT peptide contains the largest number of amino acids – 49. The T1.2 peptide is similar to the membrane-active peptide BAMA Bos taurus, which opens prospects for its use as a part of antibacterial agents or as a BAS carrier. In colostrum hydrolyzate the T1 peptide has the T highest concentration (2.04 mg/ml). It consists of 17 amino acids and matches the peptide NCI_CGAP_Brn23 cDNA Homo sapiens clone, which is similar to TR: O35085 ARX HOMEOPROTEIN. The homeobox protein containing the development gene of a PRD-like class is related to the T(1) peptide. This gene in humans is located on the X chromosome and is involved in the central nervous system and pancreas development. According to the results, the researchers isolated peptides from colostrum hydrolyzate, and identified some of them. Still a man understudies the biological role of most of the determined peptides, and has not proved its safety. In this regard, the study aimed at assessing the hydrolyzate cytotoxicity using the Neutral Red Assay method on the HEK293 cell line during a 24-hour incubation. Cytotoxicity in the first, second and third measurements at a concentration of 0.1 mg/ml was: −3.3; −1.0 and 0.20; at a concentration of 0.3 mg/ml: −1.3; −3.5 and −8.7; at 0.5 mg/ml: −2.6; −0.6 and 0.4. The results indicate no toxicity. The authors studied the antimicrobial and antifungal hydrolyzate activity. Colostrum hydrolyzate demonstrates antimicrobial activity against E. coli and B. subtilis bacteria and antifungal activity against C. albicans. The results assert that the peptides are of clinical importance and can serve as a template for the anti-infective drug development.
本研究涉及初乳水解产物胰蛋白酶肽的分析。作者分离出7个肽:TT1、TT2、TT3、T(1)、T1.1、T1.2、mpT。他们用质谱法测定了肽的分子量和肽链上的氨基酸序列;肽-使用国际数据库蛋白质NCBI。mpT肽含有最多的氨基酸——49个。T1.2肽与膜活性肽BAMA Bos taurus相似,这为其作为抗菌药物的一部分或作为BAS载体开辟了前景。初乳水解液中T1肽T浓度最高(2.04 mg/ml)。它由17个氨基酸组成,与肽NCI_CGAP_Brn23 cDNA智人克隆相匹配,与TR: O35085 ARX HOMEOPROTEIN相似。含有prd样类发育基因的同源盒蛋白与T(1)肽有关。人类的这种基因位于X染色体上,参与中枢神经系统和胰腺的发育。根据研究结果,研究人员从初乳水解物中分离出多肽,并对其中一些进行了鉴定。然而,大多数已确定的多肽的生物学作用尚未得到充分研究,其安全性尚未得到证实。因此,本研究旨在利用中性红法对HEK293细胞系进行24小时孵育,以评估水解产物的细胞毒性。在浓度为0.1 mg/ml时,第一、第二和第三次测量的细胞毒性为:−3.3;−1.0和0.20;浓度为0.3 mg/ml时:−1.3;−3.5和−8.7;0.5 mg/ml:−2.6;−0.6和0.4。结果表明无毒性。研究了水解产物的抗菌和抗真菌活性。初乳水解产物对大肠杆菌和枯草芽孢杆菌具有抗菌活性,对白色念珠菌具有抗真菌活性。结果表明,这些肽具有重要的临床意义,可作为抗感染药物开发的模板。
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引用次数: 0
Plant-Based Fermented Product Manufacture Using Lactic Acid Bacteria Cultures 利用乳酸菌培养物生产植物发酵产品
Q Engineering Pub Date : 2022-12-21 DOI: 10.29141/2500-1922-2022-7-4-7
Dmitry Khrundin, Dilyara Miassarova
The identification of new sources and food products range expansion of combined type or completely plant-based are due to the need to exclude some components from them. The research aimed at development of a new combined composition product by biotechnology methods and studying its properties. The research objects were the following: cow's milk (1.5 w/w fat %), plant “milk” (1.5 w/w fat %), commercial starter cultures of various compositions. The samples contained from 0 (the control) to 1 % plant “milk”. A man run fermentation for 12 hours at a temperature of 38–40 °С, then refrigerated samples for 24 hours to form a clot. The researchers determined titratable acidity according to the GOST 3624-92; physical and chemical indicators – by the NIR-analyzer “Infraljum FT-12”; rheological indicators – using the viscometer “Rotational Viscometer RM-1”. They revealed that the threshold titratable acidity value (75 °T) was overcome in experimental samples 2–4, in addition to the control. Acidity values varied from 58 to 72 °T depending on the starter. All samples received a satisfactory assessment in terms of physicochemical and organoleptic parameters. There was an increased content of protein and fat in the experimental samples compared to the control one due to an increase of the plant component in the product content. According to the rheological studies results, there was a clot formation with certain viscous and thixotropic properties in the product. To expand the assortment and enrich traditional fermented milk products with plant components, it is possible to use milk and plant “milk” on a 50:50 ratio and 25:75 ratio using starter cultures “ProBioYogurt” or “Yogurt”.
新来源的识别和组合型或完全植物性食品范围的扩大是由于需要排除其中的某些成分。本研究旨在利用生物技术方法开发一种新的复合产品,并对其性能进行研究。研究对象为:牛奶(1.5 w/w脂肪%)、植物“奶”(1.5 w/w脂肪%)和不同成分的商业发酵剂。样品中植物“奶”的含量从0(对照)到1%不等。一个人在38-40°С的温度下发酵12小时,然后将样品冷藏24小时以形成凝块。研究人员根据GOST 3624-92测定了可滴定酸度;理化指标-采用nir分析仪“Infraljum FT-12”;流变指标-使用粘度计“旋转粘度计RM-1”。他们发现,除了对照外,实验样品2-4也克服了可滴定酸度的阈值(75°T)。酸度值根据发酵剂的不同从58°T到72°T不等。所有样品在物理化学和感官参数方面都得到了满意的评估。由于产品中植物成分含量的增加,实验样品中的蛋白质和脂肪含量较对照样品有所增加。根据流变学研究结果,产物中存在具有一定粘性和触变性的凝块。为了扩大品种和丰富植物成分的传统发酵乳制品,可以使用牛奶和植物“牛奶”以50:50和25:75的比例使用发酵剂“ProBioYogurt”或“酸奶”。
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引用次数: 0
Flavor Formation of Stable Double Emulsion Systems with Encapsulated Biologically Active Substances 包封生物活性物质的稳定双乳液体系的风味形成
Q Engineering Pub Date : 2022-12-21 DOI: 10.29141/2500-1922-2022-7-4-9
O. Feofilaktova, N. Zavorokhina
Ensuring the functional food ingredients stability and the flavor formation of foods fortified with them meets the healthy nutrition principles, which contributes to the human and future generations health, as well as reduces the disease risk. The research aim was to study the biologically impact of the active substances encapsulation into double emulsion food systems (DEFS) on the organoleptic profile formation and the biologically active substances resistance to oxidation. The study demonstrated the comparative organoleptic analysis results of the inverse emulsion with fish oil and DEFS o/w/o with fish oil encapsulated in the internal phase of the emulsion matrix; direct emulsion with vitamin B1 (thiamine) and DEFS w/o/w with vitamin B1 encapsulated in the internal phase of the emulsion matrix; as well as the oxidation stability study results of fish oil in the composition of the inverse emulsion and dual emulsion system during storage. A man compiled organoleptic profiles of control samples and DEFS with encapsulated BAS. The results showed that the fish oil and vitamin B1 encapsulation in DEFS contributed to the color and flavor correction – deodorization and elimination of fish oil and vitamin B1 aftertaste and off-flavours. Study confirmed the effectiveness of fish oil encapsulation in DEFS, expressed in increasing its resistance to oxidation. During storage, the fat oxidation intensity in the control sample was higher than in the double emulsion system. The peroxide value in the control sample increased 6 times during storage and amounted to 7.9 mmol of active oxygen per 1 kg of fat, while in the double emulsion food system the increase was only 4 times, from 1.3 on the first day of storage to 5.1 on the 35th day. On average, for every 7 days of storage the peroxide value grew by 1.32 for the control sample and by 0.76 for the double emulsion system.
保证功能性食品成分的稳定性和强化食品风味的形成符合健康营养原则,有利于人类和后代的健康,降低疾病风险。本研究旨在研究双乳食品体系(DEFS)中活性物质包封对食品感官轮廓形成及活性物质抗氧化能力的生物学影响。对鱼油反相乳化液与鱼油包封于乳化液基质内相的DEFS o/w/o进行对比感官分析;维生素B1(硫胺素)直接乳状液和DEFS w/o/w,其中维生素B1包封于乳状液基质内相;以及鱼油在反乳和双乳体系下贮存过程中氧化稳定性的研究结果。一名男子用封装的BAS编制了对照样品和DEFS的感官概况。结果表明,鱼油和维生素B1在DEFS中的包封有助于鱼油和维生素B1的色香味校正-除臭,消除鱼油和维生素B1的余味和异味。研究证实了鱼油胶囊在DEFS中的有效性,表现在增强其抗氧化能力。在贮藏过程中,对照样品的脂肪氧化强度高于双乳体系。对照样品的过氧化值在贮藏期间增加了6倍,达到每1 kg脂肪7.9 mmol的活性氧,而双乳食品体系的过氧化值仅增加了4倍,从贮藏第1天的1.3增加到第35天的5.1。平均每7天,对照样品的过氧化值增加1.32,双乳体系的过氧化值增加0.76。
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引用次数: 0
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Agro Food Industry Hi-tech
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