On the hand of a small number of patients with coronary bypasses the influence of heart-lung-machine-operations on the neopterin level in blood, generally used for the detection of rejected heart transplants, is investigated. The results suggest, that the Neopterin level is not affected by the surgical attempt.
Human hypercholesterolemic serum increases proliferation of human umbilical endothelial cells in vitro. This might be due to an increased content of low molecular weight growth factors, which are released by blood cells, mainly thrombocytes. Hypercholesterolemia could increase endothelial cell turnover and expression of dysfunctional cells which may support atherogenic processes.
The laboratory contribution in the care of a seriously ill child is essential to plan and organize the therapy after the first-step emergency care and to know the aethiology of the illness. The most acute syndromes in pediatric emergency care are: coma, convulsions, dehydration, metabolic disequilibrium, hypovolemic or anaphylactic shock, serious infectious diseases and chemical or drug poisoning. The laboratory tests which have to be available within few minutes are blood cell count, hemogasanalysis, sodium, potassium and calcium, glucose. Total proteins, serum creatinine and urea, bleeding tests, blood smear, sedimentation rate, ALT, AST, osmolality, urinary electrolytes and creatinine and cerebrospinal fluid examination should be available within sixty minutes. New accurate and rapid techniques and instrumentations make easier the diagnostic and therapeutical approach to pediatric emergency.
After isoelectric focusing of proteins of human pure pancreatic juice following digestive enzymes and zymogens were identified by substrate specific detection methods: in the anodic range trypsinogen 1, and acidic form of proelastase and procarboxypeptidase A; in the pH--range of 5.0-6.0 a trypsinogen--like protein and the secretory trypsin inhibitor; in the pH--range of 6.0-7.0 one form of each of procarboxypeptidase B, trypsinogen 2, lipase, chymotrypsinogen and two forms of alpha-amylase and in the cathodic range an activated form of procarboxypeptidase B, one form of prophospholipase A2 and an additional form of proelastase. In order to characterize glycoproteins the IEF separated pancreatic proteins were transferred onto nitrocellulose membranes. Using the periodic acid/Schiff's reagent (PAS) procedure five PAS-positive proteins (pl s 4.5; 6.0; 6.6; 6.9 and 7.3) were detected. Three Con A-binding proteins (pl s 6.0; 6.6 and 6.9) were identified by incubating the blot in Con A followed by peroxidase and o-phenylendiamin as a chromogenic peroxidase substrat.
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