American journal of translational research最新文献

MUS81 has been recognized as a significant tumor suppressor - essential for DNA damage repair and maintaining chromosomal stability. However, its biological function and expression profile in breast cancer (BC) remain unclear. In this investigation, we examined the relationship between MUS81 expression and the proliferative and migratory capacities of BC cells. MUS81 mRNA and protein levels were markedly lower in breast cancer tissues and cell lines than in adjacent normal tissues and non-tumorigenic MCF-10A cells. Functional assays revealed that MUS81 overexpression suppressed, while MUS81 silencing enhanced, BC cell proliferation and motility, as demonstrated by CCK-8, colony formation, wound-healing, and Transwell experiments. In vivo, MUS81 overexpression markedly reduced Ki-67 expression in xenograft tumors. Although MUS81 did not alter CDKN2A mRNA expression, immunohistochemistry and Western blot analyses showed that p16^INK4a protein levels increased following MUS81 overexpression. Furthermore, the modulation of p16^INK4a expression by MUS81 was abolished by pretreatment with cycloheximide or MG132, suggesting that MUS81 stabilizes p16^INK4a by preventing proteasome-mediated degradation. Collectively, these findings indicate that MUS81 works as a tumor suppressor in BC by inhibiting proliferation and migration through post-translational stabilization of p16^INK4a.

[This corrects the article on p. 162 in vol. 7, PMID: 25755838.].

Objective: This study aimed to investigate the effect of comprehensive nursing intervention on postoperative gastrointestinal function and complications in patients undergoing endoscopic polypectomy.

Methods: This study was a prospective randomized controlled trial (The name of the registry of clinical trial: the impact of comprehensive nursing intervention on the postoperative gastrointestinal function and complications in patients undergoing endoscopic polypectomy; clinical triathe trial number: ChiCTR2000034984; the trial URL: https://www.chictr.org.cn/index.html). 77 patients were randomly divided into a control group (n=38, traditional conventional nursing) and an experimental group (n=39, comprehensive nursing intervention). The following outcome measures were compared: general baseline data, total compliance rate, laboratory biomarkers (inflammatory factors, nutritional indicators, gastrointestinal hormone levels), gastrointestinal symptom scores, postoperative recovery indicators, clinical risks (venous thromboembolism [VTE] and falls), adverse event rates (total adverse reactions, polyp recurrence, postoperative complications), and patient-centered outcomes (life comfort, quality of life, and nursing quality, and nursing satisfaction).

Results: After nursing interventions, the experimental group showed significant improvements across multiple metrics compared to the control group (all P<0.05). Key findings included reductions in inflammatory markers (procalcitonin, C-reactive protein, interleukin-17), faster postoperative recovery (shorter times to bowel sound recovery, first exhaust, and first bowel movement, and hospitalization), and lower clinical risks (VTE and fall scores, total incidence rate of adverse reactions and postoperative complications, and recurrence rate of polyps). Concurrently, the experimental group exhibited enhanced nutritional status (higher albumin and hemoglobin levels), improved gastric physiology (elevated pepsinogen I [PGI], PGI/PGII ratio, and lower PGII and gastrin-17), and superior patient-reported outcomes (general comfort questionnaire and gastrointestinal quality of life index scores), and nursing evaluations (quality and satisfaction).

Conclusions: Comprehensive nursing intervention could promote the recovery of gastrointestinal function in patients undergoing endoscopic polypectomy, and reduce the occurrence rate of postoperative complications.

Objective: The purpose of this study was to assess how a multidisciplinary specialized nurse intervention affected the prognosis of pediatric patients undergoing continuous renal replacement therapy (CRRT) for acute kidney injury (AKI).

Methods: A total of 140 children having CRRT (January 2022-December 2024) were divided into two groups in this single-center retrospective cohort study: a routine care group (n = 68) or a specialized nursing group (n = 72). Unplanned CRRT interruptions, complications, treatment duration, recovery of renal function, length of stay, and family satisfaction were among the outcomes that were compared.

Results: Unplanned CRRT interruptions and overall complications were significantly reduced in the specialized nursing group (25.00% vs. 85.29%, P < 0.001). Additionally, this group showed shorter total CRRT duration (46.09 ± 1.46 vs. 52.69 ± 1.40 hours), faster renal recovery (creatinine reduction: 5.26 ± 0.17 vs. 6.87 ± 0.29 hours; urine output normalization: 3.86 ± 0.14 vs. 5.07 ± 0.18 hours), shorter hospital stay (10.98 ± 0.76 vs. 14.11 ± 0.92 days), and higher family satisfaction scores (86.29 ± 3.57 vs. 67.13 ± 4.78), all P < 0.05.

Conclusion: By lowering complications, speeding recovery, minimizing hospital stays, and improving family satisfaction, the multidisciplinary specialized nursing model dramatically improves clinical outcomes in pediatric AKI patients undergoing CRRT.

Objective: A retrospective analysis of the efficacy of Shenqi Eleven-Flavor Granules combined with electrothermal warm acupuncture for spleen and kidney deficiency type related "firm but not lasting" erections.

Methods: This study utilized a retrospective cohort design to analyze the clinical data of 105 patients presenting with erection that is firm but not lasting, attributed to deficiency of both spleen and kidney. These patients were categorized into three groups, each consisting of 35 cases: the electroacupuncture group, the Yougui electroacupuncture group, and the Shenqi electroacupuncture group. The overall efficacy, International Index of Erectile Function (IIEF), Erectile Hardness Score (EHS), Erectile Quality Score, serum testosterone levels (T), and Traditional Chinese Medicine (TCM) Syndrome Score were analyzed among the three groups of patients.

Results: Compared to the electroacupuncture and Yougui-electroacupuncture groups, the total effective rate, IIEF-5 score, EHS, EQS, T level, and satisfaction rate with erection duration during sexual activity were all higher in the Shenqi-electroacupuncture group (all P<0.05). Conversely, the TCM syndrome score was significantly lower in the Shenqi-electroacupuncture group (P<0.05).

Conclusion: Shenqi Eleven-Flavor Granules combined with electrothermal warm acupuncture demonstrates definite clinical efficacy in the treatment of "firm but not lasting" erectile dysfunction due to spleen and kidney deficiency. This combined therapy effectively improves erectile function, prolongs erection duration, reduces TCM syndrome scores, and shows no serious adverse reactions.

Objective: To compare the clinical outcomes of endoscopic sinus surgery (ESS) combined with vidian neurectomy versus ESS alone in patients with allergic rhinitis and chronic rhinosinusitis, and to identify factors associated with postoperative recurrence.

Methods: A total of 375 patients with allergic rhinitis and chronic rhinosinusitis treated between 2019 and 2024 were included in this study. Patients were grouped into either the ESS alone (conventional treatment group; n=194) or the ESS with vidian neurectomy (combined treatment group; n=181) groups based on their treatment protocols. Propensity score matching (PSM; 1:1) was applied to balance baseline characteristics. Perioperative metrics, including nasal function, inflammatory and immune markers, as well as symptom and quality-of-life scores were compared between the two groups. Recurrence within 12 months postoperatively was recorded. Multivariate logistic regression analysis was performed to identify independent risk factors for disease recurrence.

Results: After PSM, baseline characteristics were comparable between the two groups. Compared with the conventional treatment group, the combined treatment group showed a shorter operative time (median 34 vs 45 min, P<0.001) and lower postoperative nasal resistance (0.16 vs 0.21 Pa/(cm³·s), P<0.001). Additionally, the combined treatment group demonstrated significantly lower IL-6 levels (11.16±3.68 vs 12.61±3.62 ng/L, P<0.001), accompanied by a greater reduction in C-reactive protein (CRP) and a more pronounced increase in IFN-γ. Endoscopic and patient-reported scores improved more, including Lund-Kennedy and SNOT-22. The overall recurrence rate was 19.2% (72/375), with the combined treatment group reporting significantly lower recurrence rates. Multivariate analysis identified ESS alone (OR=6.017, P=0.003), smoking (OR=3.82, P=0.022), higher preoperative IL-4 (OR=1.46, P<0.001), higher eosinophil percentage (OR=5.932, P<0.001), and higher SNOT-22 scores (OR=1.442, P<0.001) as independent predictors of recurrence.

Conclusion: The addition of vidian neurectomy to ESS was associated with superior improvements in symptoms and nasal function, modulation of inflammatory responses, and a reduced 12-month recurrence rate. Preoperative IL-4, eosinophils, SNOT-22 score, and smoking status are closely associated with disease recurrence.

Objective: To explore the effects of a semiconductor laser combined with compound chlorhexidine and dexamethasone membrane in the treatment of chronic periodontal disease, and its effects on periodontal health and gingival crevicular fluid inflammatory factors.

Methods: The clinical data of 100 patients with chronic periodontal disease were retrospectively analyzed. According to different treatment methods, they were divided into a conventional treatment group (n=46, receiving conventional periodontal basic treatment) and a combined treatment group (n=54, receiving conventional treatment plus semiconductor laser and compound chlorhexidine-dexamethasone membrane). Periodontal health-related indicators, gingival crevicular fluid inflammatory factor levels, and Oral Health Impact Profile-14 (OHIP-14) scores were compared before and after treatment. The clinical efficacy and occurrence of adverse reactions were evaluated.

Results: After treatment, the plaque index, probing depth, sulcus bleeding index, attachment loss, interleukin-6, high-sensitivity C-reactive protein and tumor necrosis factor-αlevels were lower than before treatment, and the OHIP-14 scores were improved (all P<0.05), with significantly more improvement in the combined treatment group (all P<0.05). The conventional treatment group had higher overall effective rate, shorter symptom improvement time (gingival swelling and pain, bad breath, loose teeth) and lower incidence of adverse reactions (all P<0.05).

Conclusion: Semiconductor laser combined with compound chlorhexidine and dexamethasone membrane has definite clinical effects for chronic periodontal disease. It can effectively improve periodontal health, reduce the levels of inflammation in gingival crevicular fluid, improve patients' quality of life, and is highly safe.

Aims: To evaluate the long-term survival outcomes and therapeutic efficacy of vemurafenib combined with iodine-131 (¹³¹I) by dual pathways in patients with lymph node metastatic BRAF-mutant thyroid cancer.

Methods: This retrospective study included 80 patients with lymph node metastatic BRAF V600E-mutant thyroid cancer, treated between January 2022 and January 2025. Patients were assigned to a control group (vemurafenib monotherapy, n = 42) or an observation group (vemurafenib combined with ¹³¹I, n = 38). Progression-free survival (PFS), overall survival (OS), and metastasis-free survival (MFS) were compared. Survival analyses were performed using Kaplan-Meier curves.

Results: Kaplan-Meier survival analysis showed a significantly lower PFS, OS, and MFS in the control group, compared to the observation group (all P<0.001). Treatment with combination therapy led to significantly superior thyroid function recovery, with lower thyroid-stimulating hormone levels as well as higher free T4, free T3, and total T3 levels in the observation group (all P<0.001). Immune analysis showed significantly enhanced activation of CD8+ T cells and a reduction in regulatory T cells (both P<0.001).

Conclusion: The vemurafenib combined with ¹³¹I revealed superior efficacy in improving survival and thyroid function recovery in lymph node metastatic BRAF-mutant thyroid cancer.

Psychiatric nursing can involve patient agitation, emotional labor, and difficult ethical situations, which are prone to nurse burnout and job-related stresses. Mindfulness-based interventions (MBIs), such as mindfulness-based stress reduction (MBSR) and acceptance and commitment therapy (ACT), could help alleviate such challenges. This narrative review brings together qualitative and quantitative research to examine the description of MBIs concerning aspects of stress, burnout and resilience in psychiatric nurses. Across clinical and cultural contexts, longer and more structured mindfulness programs - especially those shaped around local expectations - show clearer reductions in burnout dimensions. Shorter programs, single-session workshops, or online-only formats offer more limited benefits, and improvement often doesn't last long. Also, the MBSR is linked to better attentional control and greater emotional awareness, while ACT is associated with increased psychological flexibility and coping that reflects personal values - effects that seem especially meaningful in collectivist or spiritually influenced settings. Even so, findings on personal accomplishment and longer-term resilience are mixed because many studies rely on small samples, short follow-up, or weaker study designs. Even with these limitations, the literature suggests that integrating MBIs into organizational practices - through strategies such as protected time, managerial support, and culturally adapted materials - may help strengthen psychiatric nurses' well-being and foster more sustainable mental-healthcare environments. Research using stronger comparative designs is needed to explore how demographic and cultural factors influence the reach and relevance of MBIs for psychiatric nurses.

Objective: To investigate the protective effects and molecular mechanisms of electro-acupuncture (EA) in a cyclophosphamide (CTX)-induced rat model of premature ovarian insufficiency (POI), focusing on ovarian microRNA (miRNA) regulation and TGFβ/Smad pathway modulation.

Methods: Twenty-four female Sprague-Dawley rats were classified into four groups: Control, CTX, CTX + EA, and CTX + GnRHa (positive control). POI was induced by CTX injection. EA was applied at six acupoints for 15 min daily (days 10-25). Ovarian function was evaluated through estrous cycle monitoring, histology, follicle counting, and serum hormone assays (FSH, E2, AMH). Serum ALT, AST, BUN, and UA were measured to assess organ toxicity. Ovarian ultrastructure was analyzed by TEM. Differential miRNA expression was profiled by small RNA sequencing and validated by qRT-PCR. Pathway analysis (KEGG, GO) and TGFβ/Smad signaling evaluation (qRT-PCR, western blot) were performed.

Results: EA significantly improved body weight and ovarian index (P < 0.01 vs. CTX) and reduced CTX-induced hepatic and renal injury markers. Regular estrous cycles were restored in 83% of EA-treated rats. EA normalized FSH, E2, and AMH levels (P < 0.01 vs. CTX), restored follicle counts and ovarian morphology, and reversed subcellular damage observed under TEM. EA upregulated protective miRNAs (miR-145-3p, miR-25-3p) and inhibited aberrant TGFβ/Smad activation, revealing a miRNA-mediated mechanism underlying ovarian protection.

Conclusion: EA confers significant protection against CTX-induced POI by preserving ovarian structure, normalizing endocrine function, and regulating miRNA-TGFβ/Smad signaling, supporting its potential as a non-pharmacological therapy for ovarian preservation and fertility maintenance.