American journal of veterinary research最新文献

Objective: The objective of this study was to investigate the differential expression of genes associated with coagulation in bovine monocyte-derived macrophages (MoMΦ) exposed to lipopolysaccharide (LPS) in vitro. We hypothesized that MoMΦ stimulated with LPS would have upregulation of procoagulant genes and downregulation of genes protecting against coagulation.

Methods: MoMΦ were isolated from Holstein steers and exposed to Escherichia coli-derived LPS or a control for 3 hours. We used transcriptomics (RNA sequencing) to characterize the differential expression of genes associated with coagulation in the LPS-exposed MoMΦ relative to the control group.

Results: 1,602 genes were upregulated and 1,209 genes downregulated 3 hours after exposure to LPS. Monocyte-derived macrophages exposed to LPS displayed statistically significant upregulation of 4 proinflammatory genes, 2 anti-inflammatory genes, 8 genes involved in promoting coagulation, and 5 genes considered protective against coagulation. There was significant downregulation of 1 gene involved in the promotion of coagulation.

Conclusions: Our results showed increased expression of most genes investigated promoting and protecting against coagulation and increased expression of proinflammatory and anti-inflammatory genes. These findings suggest that MoMΦ exhibit a multifaceted response in the early response to LPS, promoting and protecting against excessive coagulation. This multifaceted response highlights the interplay between different pathways involved in early sepsis.

Clinical relevance: Our data demonstrate the utility of using MoMΦ as a model system to investigate sepsis-associated coagulopathies. These insights into the early transcriptomic changes in response to LPS may help guide future research on the development of treatment modalities or diagnostic tests for patients with sepsis.

Objective: To investigate the level of whole-blood ionized magnesium (iMg) in dogs with long-term use of proton pump inhibitors (PPIs).

Methods: The study included 10 client-owned dogs with esomeprazole administration over 6 months and 62 healthy dogs to determine de novo reference interval (RI) of iMg. Dogs that received esomeprazole for 6 months or longer were retrospectively reviewed to determine the incidence of hypo- or hypermagnesemia based on the de novo RI. Additionally, the iMg levels from the study group were compared with those of 20 age-, sex-, and body weight-matched controls from the 62 dogs.

Results: The median (range) duration of esomeprazole usage was 26 months (6 to 94). The de novo RI for iMg was determined as 0.73 (90% CI, 0.58 to 0.87) to 1.43 mg/dL (90% CI, 1.33 to 1.46). Based on the RI, none of the dogs with long-term esomeprazole developed hypo- or hypermagnesemia. The iMg from the matched control group was 1.17 mg/dL (90% CI, 0.83 to 1.46). The lowest iMg after 6 months of esomeprazole administration (90% CI, 0.96 mg/dL, 0.87 to 1.41) was significantly lower than the control group (P = .031). The iMg measured at the end of long-term esomeprazole treatment was 1.03 mg/dL (90% CI, 0.87 to 1.41) and not significantly different from the control group (P = .179).

Conclusions: Ionized hypomagnesemia was not observed after long-term use of esomeprazole in the small number of dogs included in this study. Robust RI needs to be determined in future studies to investigate the incidence of hypomagnesemia in dogs with long-term use of PPIs.

Clinical relevance: Future studies in a larger number of dogs are warranted to confirm the findings from the present study and to determine whether the long-term use of esomeprazole in dogs is at risk of developing ionized hypomagnesemia.

Objective: To determine the feasibility of catheterizing the portal vein to obtain serial portal vein blood samples in steers. We hypothesized that the portal vein catheterization would be a successful continuous sampling technique with minimal adverse effects in steers.

Methods: 2 groups of steers were used: a pilot group (n = 2) and experimental group (n = 6). In both groups, steers were sedated with xylazine. The right rib spaces were clipped and aseptically prepped. The portal vein was visualized via ultrasound, and a 14-gauge catheter was placed percutaneously and advanced into the portal vein. A guide wire was passed through the catheter, followed by a tissue dilator and then a vascular balloon catheter. In the pilot group, blood chemistries were performed prior to portal vein catheterization and then again once the catheter was placed (with samples from both the jugular vein and portal catheter). The liver was also examined at necropsy for any gross lesions in both groups.

Results: All steers tolerated the portal vein catheters well, with the catheters lasting for the full length of the study period (7 days). The only observed adverse reaction was a superficial abscess at the catheter site (n = 3). On necropsy, 1 liver had gross discoloration, but no other abnormalities were noted. There were no significant changes in biochemistry profiles before or after portal vein catheterization.

Conclusions: Portal vein catheterization is a novel and feasible serial sampling technique of the portal vein.

Clinical relevance: This technique can be used in future pharmacokinetic, nutrition, metabolism, or toxicity studies.

Objective: To assess the pharmacokinetics and sedative effects of a single administration of oral gabapentin in African cheetahs (Acinonyx jubatus) at 2 different dosages.

Methods: Adult cheetahs (n = 16) located at 3 different zoological institutions were prospectively enrolled to receive single doses of gabapentin administered at 2 different dosages (10 mg/kg and 20 mg/kg). Venipuncture was performed under behavioral restraint at predetermined time points over a 24-hour period using a sparse sampling model. Plasma concentrations of gabapentin were determined using high-performance liquid chromatography. A modified domestic felid sedation scoring system was used to assess animals at each time point by 3 masked scorers, and sedation scores were compared between time points.

Results: Mean ± SE maximal plasma concentrations were 24.0 ± 12.8 μg/mL and 31.4 ± 8.57 μg/mL for the 10- and 20-mg/kg dosages, respectively. For both dosages, concentrations remained elevated at the final collection time point of 24 hours (2.39 ± 1.97 and 3.93 ± 3.09 μg/mL for 10 and 20 mg/kg, respectively). Mild sedation was achieved for both doses up to 24 hours postadministration, with no significant differences between dosages.

Conclusions: Gabapentin was well absorbed following oral administration, and concentrations remained elevated 24 hours postadministration. Gabapentin produces mild sedation at 10 or 20 mg/kg for up to 24 hours.

Clinical relevance: Gabapentin given to cheetahs at these dosages is a useful tool for improving patient welfare due to its mild sedative effects over a clinically relevant time period.

Objective: To describe the presentation, clinical findings, diagnosis, treatment, and outcome of cases of trismus (lockjaw) in cold-stunned sea turtles.

Animals: 4 Kemp's ridley (Lepidochelys kempii) and 1 loggerhead (Caretta caretta) sea turtle.

Methods: Cold-stunned sea turtles that presented with difficulty or inability to open their jaw between 2009 and 2023 were included. Information retrieved from medical records included signalment, physical exam findings, diagnostic information, definitive diagnosis via either advanced imaging or histopathology, treatment, and clinical outcome.

Results: Turtles presented between 4 and 48 days into rehabilitation. Three were diagnosed by advanced imaging (CT or MRI), and 2 were diagnosed based on clinical signs and postmortem histopathology. Treatment was multimodal and consisted of antibiotics (5/5), nonsteroidal anti-inflammatories (5/5), vitamin E (3/5), intralesional steroid therapy (3/5), acupuncture (3/5), antifungals (2/5), anti-inflammatory parenteral steroids (2/5), physical therapy (2/5), therapeutic laser (2/5), and supportive feeding via either total parenteral nutrition (1/5), or tube feedings (2/5). Two animals were released, 2 died naturally, and 1 was euthanized.

Clinical relevance: Trismus (lockjaw) is an uncommon finding in stranded cold-stunned sea turtles that can have a significant impact on animal welfare if not diagnosed and treated. This report describes the condition to aid clinical case management and resource allocation in rehabilitation facilities.

Objective: To evaluate a predictive model's ability to determine cattle mortality following first and second treatment for bovine respiratory disease and to understand the differences in net returns comparing predictive models to the status quo.

Methods: 2 boosted decision tree models were constructed, 1 using data known at first treatment and 1 with data known at second treatment. Then, the economic impact of each outcome (true positive, true negative, false positive, and false negative) was estimated using various market values to determine the net return per head of using the predictive model to determine which animals should be culled at treatment. This was compared to the status quo to determine the difference in net return.

Results: The models constructed for the prediction of mortality performed with moderate accuracy (areas under the curve > 0.7). The economic analysis found that the models at a high specificity (> 90%) could generate a positive net return in comparison to status quo.

Conclusions: This study showed that predictive models may be a useful tool to make culling decisions and could result in positive net returns.

Clinical relevance: Bovine respiratory disease is the costliest health condition experienced by cattle on feed. Feedyard record-keeping systems generate vast amounts of data that could be used in predictive models to make management decisions. It is essential to understand the accuracy of predictions made via machine learning. However, the economic impact of implementing predictive models in a feedyard will influence adoption.

Objective: The objective of this study was to analyze the use of rebound tonometry, as a noninvasive diagnostic tool, in arachnids.

Methods: 5 juvenile (yearling) female and 1 juvenile male curlyhair tarantulas (Tliltocatl albopilosus, previously Brachypelma albopilosum) were used to track estimated ventral opisthosoma pressures over a 9-month period of time. Younger, growing animals were selected as they are more likely than adults to go through multiple molts throughout the 9 months of the study length. An iCare TONOVET TV01 rebound tonometer was used to measure the estimated ventral opisthosoma pressures of the spiders. Measurements were obtained from the ventral opisthosoma, 1 of the thinnest areas of exocuticle throughout the body. Readings were obtained once per week from the ventral opisthosoma for the first 2 months, then once every 2 weeks for 1 month, then back to once per week for the remainder of the study. Additional measurements were obtained following each ecdysis, after a 2% body weight sampling of hemolymph, and at the end of the study to evaluate readings in response to induction of general anesthesia with 5% isoflurane gas and oxygen flow at 2 L/min for 10 minutes.

Results: The average of all estimated ventral opisthosoma pressure when spiders were not in molt was 26.19 mm Hg (SD, 3.54), with a statistically significant decrease postmolt to an average of 15.31 mm Hg (SD, 3.81), followed by a gradual increase back to premolt pressures over a 3-week period with an average of 22 days (SD, 1.93). Estimated ventral opisthosoma pressures decreased post hemolymph removal. There is not sufficient evidence that estimated ventral opisthosoma pressures changed over time following the anesthesia.

Clinical relevance: This study demonstrates that tonometry can be used to generally assess the estimated ventral opisthosoma pressure, which could correlate with where a spider is in an ecdysis cycle.