Kanagawa shigaku. The Journal of the Kanagawa Odontological Society最新文献

The temporomandibular joint (TMJ) provides articulation between the jaw and cranium, which associate with jaw movement and growth. The articular disc of TMJ separates the surfaces of the temporal bone and mandibular condyle. An understanding of its biochemical composition is very important, because the TMJ exhibits variety of pathological derangements including anterior displacement of disc. Proteoglycan (PG), major component of the disc, is one of the non-collagenous protein, which relates to the tissue viscoelasticity and physiological stress. This paper describe the isolation and characterization of proteoglycans from bovine articular disc. Articular discs obtained from bovine were cutted into small pieces. They were then extracted with 0.05 M Tris-HCl buffer, pH 7.4, containing 4 M guanidium HCl (Gdm HCl) and protease inhibitors for 12h at 4 degrees C. PGs were isolated by chromatography of Gdm HCl extract. The sequential chromatography steps consisted of ion-exchange chromatography on DEAE-Sephacel in 4 M Urea, rechromatography of FPLC Superose 6 in 4 M Urea. The two forms of PGs (on SDS-PAGE, Mr = 120-130 K and 200 K) were isolated by these steps. The core protein of two forms of PGs liberated by chondroitinase ABC were shown by SDS-PAGE as Mr = 58,000. Also the glycosaminoglycan (GAG) chains of PGs liberated by papain digestion were shown by SDS-PAGE as Mr = 70-80 K. Moreover GAG chains of PGs were consisted of chondroitin sulfate A, C and dermatan sulfate. Antisera raised against bovine periodontal ligament PGs cross-react with core protein of disc PGs (obtained after chondroitinase digestion), but not with bone small PG. These data suggested that two forms of PGs have a identical core protein. However 120-130 K PG might have one GAG chain, and 200 K PG might have two GAG chains. These small PGs were different from bone small PG, especially dermatan sulfate contents, which may be important in disc tissue.

From both vital and denture sides, the design of denture in dental clinic needs taking care of various viewpoints; alleviation of the adverse effect on the periodontium is necessary on the vital side, and prevention of possible rupture of the denture at its functioning and taking hygienic measures are necessary on the denture side. Utilization of the metal and adhesive, 4-META containing resin (hereinafter referred to as adhesive resin) in the design of denture has been reported to be not only hygienically advantageous because of difficult adhesiveness of bacterial flora due to solid union between the metal and the resin, but also less in danger of the denture rupture from its finishing line portion. However, a few reports have been made on the analysis of the correlation between the mechanical strength of the whole of denture and the use of adhesive resin and on the deformation of the metallic frame due to curing shrinkage of adhesive resin. In the present study, in terms of these problems, utilizing the modal analysis applying a vibrato-logical technique and the strain gauge device appropriate for the determination of the time-course, samples of chrome-cobalt alloy and of partial denture were examined for the influence of inserted adhesive and non-adhesive resins upon them. The following results were obtained: 1. The results of the determination by the strain gauge method revealed that the insertion of adhesive resin tended to have more displacement volume for more insertion volume and for thinner chrome-cobalt alloy sample and that of non-adhesive resin had practically no adhesive force, suggesting a small deformation of the sample due to curing shrinkage, with little interlocking force. 2. The results of modal analysis revealed that the insertion of the adhesive resin onto the denture sample led to an increase in mechanical strength and a rigid body modality of the activity at the finishing line portion compared with the non-adhesive resin. 3. From the results of the determination by the strain gauge method and of the modal analysis, the application of adhesive resin was suggested to increase the intensity of denture.

The fundamental element of a latent image on a selenium plate of Electroradiography is a electrical charge. Therefore, the spatial resolution which can be expected from Electroradiography is extremely high. Here, the author developed the Pulse Electron Beam Scanner (PEBS) as the technique to obtain digital pictorial information from the latent image and obtained following results; 1. We succeeded in converting the latent image into digital signal with high accuracy by using secondary emitted electron through the apparatus that features pulsed electron beam, summing function for secondary emitted electron and plurally arranged detectors and extraction field applied grid. 2. The spatial resolution which we can enjoy to obtain through this apparatus is greater than 500 Lp/mm and the dynamic range at the time when we obtained 500 Lp/mm in terms of the voltage applied to the electrode was +60V to +110V. 3. We succeed in obtaining the digital X ray picture of the bone trabecular structure of human spongy bone with 50 power magnification without using any enlargement picture taking method. The above results indicate that converting the latent image on the selenium plate into digital signal with very high spatial resolution by using PEBS system was achieved. Therefore, we can conclude that we succeeded in obtaining the high spatial resolution digital image from the electroradiographic latent image which has the spatial resolution as high as electrical charge distribution.

Denture fabrication involves a number of complex operations that have an effect on reproduction accuracy during each step of the process. Traditionally, metal patterns act as the standard for the mechanical properties of the main materials used in denture making. This means that doubts still remain in cases where the mucous membrane, with its complex configurations, is the object of investigation. The non-contact measuring instrument using laser light (known hereafter as "measuring instrument") enabled measuring small configurations. The measuring instrument enabled measurement of physical configurations that up to now were considered impossible to measure. The fabrication process up to creation of a denture base with the mucous membrane as the basic pattern was divided into four steps for measurement: mucous membrane, impression material, plaster and resin. The measuring device simultaneously carried out qualitative and quantitative analysis in three dimensions for all steps. Processing of test materials was unnecessary so that measurements could be accomplished in a very short time. Measurements revealed a variety of features, including those that are not affected by the physical properties of the materials for measurement. It was also possible to systematically investigate the various changes that take place during denture fabrication with the mucous membrane acting as the basic pattern. The following results were obtained: 1. The experiment confirmed possible application in dentistry of a non-contact device that uses laser light to measure extremely small configurations. 2. Results hint at the possibility of quantitative measurement of objects that cannot be directly measured with conventional methods. 3. During fabrication of dentures, it is very easy for changes in the total configuration to occur when taking impressions. Minute changes in the configuration occur on the surface when taking the plaster cast. During the final stage of the resin base, the tiny configurations became flat and resembled the configuration of the impression more than the original configuration of the mucous membrane.

Mandibular lateral displacement (MLD) is a result of temporarily accelerated growth of unilateral condyle. Structure of lower face of typical mandibular lateral displacement malocclusion was analysed by means of modified denture frame analysis. Results of measurement indicated that noteworthy present on difference of maxillary occlusal plane between right and left dentitions. It suggested that the MLD malocclusion result from unilateral elongation of the molars due to posterior discrepancy and functional displacement of mandible associated with the differential condylar growth.

During the postnatal development of the rat submandibular gland, the origin of the membranes of the cytoplasmic vacuoles in terminal tubule cells was studied by light and electron microscopy. The terminal tubule cells before weaning contained the extensive endoplasmic reticulum, the small Golgi apparatus and abundant granules. Slight vacuolization was seen in the perinuclear area just before weaning, and after weaning the vacuoles rapidly increased in number. Acid phosphatase activity was localized in lysosomes and in newly formed vacuoles. The enzyme reaction product was also observed in the vesicles within the vacuoles. Using unbuffered osmium method, very intense staining was observed in the newly formed vacuoles as well as in the outer cisternae, the vesicles of Golgi apparatus and frequently in cavity between the double membranes of the endoplasmic reticulum. The newly formed vacuoles were a double membrane surrounding sacs which contained clearly recognizable cell organelles and/or secretory granules. Within the large vacuoles several organelles were present. Therefore, the cytoplasmic vacuoles in terminal tubule cells corresponded to crinophagy. These results suggest that the autophagic vacuoles in terminal tubule cells are derived from the endoplasmic reticulum and fused with lysosomes and then the inner membrane is gradually digested.

An HB prophylaxis vaccination that included a primary and secondary vaccination was carried out on persons working at the university hospital. In the primary vaccination group, the subjects were inoculated the second time with a vaccine derived from human blood plasma obtained from the Kitazato Therapeutic Research Institute. The third time, they were inoculated with a vaccine from the Chemo-Sero Therapeutic Research Institute derived from a second-generation vaccine organized ferment. The vaccine used for the second inoculation group was obtained from the Chemo-Sero Therapeutic Research Institute. Results were summarized as follows: 1) The primary vaccination group: (1) The number of the 153 subjects inoculated with the primary vaccination at the time of the second inoculation, 49 subjects (32%) tested positive for antibodies 3-4 months following inoculation. Thirty-two subjects (20%) tested positive 15-16 months following inoculation with a high rate among females. (2) Among 104 subjects inoculated the third time with the primary vaccination (those who tested negative the second inoculation of the above vaccine), 61 subjects (58.7%) tested positive for antibodies 8-9 months following inoculation with a generally high rate among females. Twenty-eight subjects (26%) tested 15-16 months after inoculation with a high rate among females between 20 and 30 years and among males between 40 and years. 2) The secondary group: Among the 38 subjects who were inoculated the second time with the secondary vaccination, 8 subjects (21%) tested positive for antibodies 5-6 months following inoculation, with a high rate among females in their 20's. 3) The rate of antibodies formation.(ABSTRACT TRUNCATED AT 250 WORDS)

Although the orthodontist is constantly aware of the developing third molar and its possible effects on the dentition during and after orthodontic treatment, the relationship between the third molar and the development of malocclusion had not been resolved. To attempt to clarify some of the problems associated with the third molar, an analysis of the factor in fluencing on the dentition and denture frame was performed. In this study, twenty-one adult cases which had no prothodontic correction of the tooth shape and orthodontic treatment with at least three wisdom teeth were used for evaluation of the denture frame structure and occlusion. The case which impacted third molar (M3 impacted group) showed less than 25 degree mandibular plane angle (FH-MP) without exception, while the case which the third molar erupted (M3 erupted group) were able to divide into high angle (more than 30 degree of FH-MP) and low angle (less than 29 degree of FH-MP) groups. The M3 impacted with low angle group and M3 erupted with low angle group showed relatively normal occlusion and favorable denture frame structure, but M3 erupted with high angle group indicated that the denture frame composition was affected by posterior discrepancy especially the steepness of occlusal plane which might be the over eruption of posterior teeth. The M3 erupted with high angle group also included two cases of severe anterior open-bite with skeletal deformity. These findings suggest that the posterior discrepancy due to existence of third molars influence on the dento-facial-skeletal structure and development of malocclusions.

Synthetic hydroxyapatite (HA) powders were treated for varying time (1-60 min.) with neutral sodium fluoride solution of varying concentrations (10(0)-10(4) ppm). The alkali-soluble and -insoluble fluorides formation were studied independently of each other following their separation in molar potassium hydroxide. The total fluoride uptake by HA and its alkali-soluble and/or -insoluble fluoride contents were both found to be linearly related to the logarithm of the ionic fluoride level in the treatment solution, except for the outstandingly high figures in 10(4) ppm F solution. Further, the alkali-soluble/-insoluble fluoride rates were found in the same manner above. From the present results, it was suggested that the less treatment period (e.g. 1 min.) could sufficiently be effective in the fluoride painting. Further, the usefulness of frequent application with lower fluoride concentration was also suggested.

An experiment using modal analysis was used to ascertain the activity of complete dentures and artificial teeth. Three types of artificial teeth were used: a porcelain tooth, a resin tooth and a hard resin tooth. Animation and displacement values were used as criteria in determining the influence that differences in tooth materials had on the activity of the complete denture. The experiment was conducted to act as an aid in denture design and the following results were obtained: 1. The resonance frequency for the different test materials ranged from mode 4 to mode 7. 2. Regarding displacement value, a value of 1 micron or more was obtained in the 1 octave bandwidth for the 250 Hz band, 500 Hz band and the 1 kHz band. Among these, maximum displacement for the upper complete denture was as follows: When a porcelain tooth was used and a vibration of 1 for mode 1 (197 Hz) was applied, the value for the 3 cusp tip was 39.48 microns and that for the labial denture border side was 43.35 microns. As for the lower complete denture, when a porcelain tooth was used and 5 oscillation was applied for mode 1 (309 Hz), the value for the 3 cusp tip was 68.38 microns and that for the rear part of the denture border was 139.2 microns. 3. Regarding the activity of the complete denture in the 250 Hz and 1 kHz bands, when a porcelain tooth was used, a curve mode was observed for the upper complete denture in the labiolingual direction with the cervical line as the border. When the resin tooth and hard resin tooth were used, it was difficult to recognize any change with the cervical line as the border. It was also difficult to recognize a change at the cervical section for the lower complete denture. 4. As for the 500 Hz band, when a porcelain tooth and a resin tooth were used, there were frequencies (300 Hz-400 Hz) at which twisting in a labiolingual direction at the cervical section was recognized for the upper complete denture as well as frequencies (400 Hz-600 Hz) where this was not recognized. A rigid body mode was recognized when the hard resin tooth was used. In addition, differences in the location for applying for vibration had an influence on the activity of the lower complete denture.