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Obituary: in memory of the scientific career of Professor Roger C Harris
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-03-04 DOI: 10.1007/s00726-025-03446-5
Wim Derave, Paul Greenhaff, Pat Harris, Jay Hoffman, Kent Sahlin, Craig Sale, Bryan Saunders, David Snow
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引用次数: 0
Unveiling the tissue-specific landscape of nuclear-encoded mitochondrial genes involved in amino acid  metabolism in buffalo
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-28 DOI: 10.1007/s00726-025-03447-4
E. M. Sadeesh, Madhuri S. Lahamge

Mitochondria play a pivotal role in energy production, metabolism, and cellular signaling, serving as key regulators of cellular functions, including differentiation and tissue-specific adaptation. The interplay between mitochondria and the nucleus is crucial for coordinating these processes, particularly through the supply of metabolites for epigenetic modifications that facilitate nuclear-mitochondrial interactions. To investigate tissue-specific mitochondrial adaptations at the molecular level, we conducted RNA sequencing data analyses of kidney, heart, brain, and ovary tissues of female buffaloes, focusing on variations in mitochondrial gene expression related to amino acid metabolism. Our analysis identified 82 nuclear-encoded mitochondrial transcripts involved in amino acid metabolism, with significant differential expression patterns across all tissues. Notably, the heart, brain, and kidney—tissues with higher energy demands—exhibited elevated expression levels compared to the ovary. The kidney displayed unique gene expression patterns, characterized by up-regulation of genes involved in glyoxylate metabolism and amino acid catabolism. In contrast, comparative analysis of the heart and kidney versus the brain revealed shared up-regulation of genes associated with fatty acid oxidation. Gene ontology and KEGG pathway analyses confirmed the enrichment of genes in pathways related to amino acid degradation and metabolism. These findings highlight the tissue-specific regulation of mitochondrial gene expression linked to amino acid metabolism, reflecting mitochondrial adaptations to the distinct metabolic and energy requirements of different tissues in buffalo. Importantly, our results underscore the relevance of mitochondrial adaptations not only for livestock health but also for understanding metabolic disorders in humans. By elucidating the molecular mechanisms of mitochondrial function and their tissue-specific variations, this study provides insights that could inform breeding strategies for enhanced livestock productivity and contribute to therapeutic approaches for human metabolic diseases. Thus, our findings illustrate how mitochondria are specialized in a tissue-specific manner to optimize amino acid utilization and maintain cellular homeostasis, with implications for both animal welfare and human health.

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引用次数: 0
Serum amino acid alterations in hyperuricemia: potential targets for renal disease prevention
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-18 DOI: 10.1007/s00726-025-03444-7
Qinglin Sheng, Yuqing Ma, Bingjie Geng, Jiahui Chen, Junfei Cheng, Su Liu, Rui Li, Xiangtong Li, Jing Wang, Hongtao Lu, Fangyuan Gao, Fu Gao

Observational studies have linked uric acid (UA) levels and kidney disease to amino acid homeostasis, but the causal relationship is unclear. This study aims to determine if elevated UA affects amino acid levels and whether amino acids mediate this relationship, focusing on the causal links between UA, circulating amino acids, and kidney disease. Methods: This study utilized Uox-KO mice as a hyperuricemia model, assessed renal injury through blood biochemistry and pathology, analyzed serum amino acid changes via targeted amino acidomics, and employed Mendelian randomization to investigate the causal links between uric acid, amino acids, and renal disease. Results: Hyperuricemia Uox-KO mice have significantly higher serum UA and renal impairment markers, with histopathological analysis showing extensive renal tissue damage. Changes in amino acid balance were found in the mice's serum, with key metabolites like alanine, isoleucine, leucine, aspartic acid, cysteine, glutamate, and glycine potentially influencing UA pathophysiology. Genetically predicted UA was positively correlated with chronic renal failure (CRF) and blood urea nitrogen(BUN) levels and negatively with serum cystatin C (eGFRcys) and serum creatinine (eGFRcrea). Alanine (Ala) mediated the effect of UA on elevated CRF and BUN risk, accounting for 4.5% of the UA-CRF relationship and 14.4% of the UA-BUN association. Conclusion: In hyperuricemia mice, serum amino acids undergo metabolic changes. Genetically predicted UA levels are positively linked to CRF and BUN, but negatively linked to eGFRcys and eGFRcrea. Ala mediates UA's effect on CRF and BUN risk, indicating Ala could be a target for preventing renal diseases caused by hyperuricemia.

Graphical abstract

{"title":"Serum amino acid alterations in hyperuricemia: potential targets for renal disease prevention","authors":"Qinglin Sheng,&nbsp;Yuqing Ma,&nbsp;Bingjie Geng,&nbsp;Jiahui Chen,&nbsp;Junfei Cheng,&nbsp;Su Liu,&nbsp;Rui Li,&nbsp;Xiangtong Li,&nbsp;Jing Wang,&nbsp;Hongtao Lu,&nbsp;Fangyuan Gao,&nbsp;Fu Gao","doi":"10.1007/s00726-025-03444-7","DOIUrl":"10.1007/s00726-025-03444-7","url":null,"abstract":"<div><p>Observational studies have linked uric acid (UA) levels and kidney disease to amino acid homeostasis, but the causal relationship is unclear. This study aims to determine if elevated UA affects amino acid levels and whether amino acids mediate this relationship, focusing on the causal links between UA, circulating amino acids, and kidney disease. Methods: This study utilized Uox-KO mice as a hyperuricemia model, assessed renal injury through blood biochemistry and pathology, analyzed serum amino acid changes via targeted amino acidomics, and employed Mendelian randomization to investigate the causal links between uric acid, amino acids, and renal disease. Results: Hyperuricemia Uox-KO mice have significantly higher serum UA and renal impairment markers, with histopathological analysis showing extensive renal tissue damage. Changes in amino acid balance were found in the mice's serum, with key metabolites like alanine, isoleucine, leucine, aspartic acid, cysteine, glutamate, and glycine potentially influencing UA pathophysiology. Genetically predicted UA was positively correlated with chronic renal failure (CRF) and blood urea nitrogen(BUN) levels and negatively with serum cystatin C (eGFRcys) and serum creatinine (eGFRcrea). Alanine (Ala) mediated the effect of UA on elevated CRF and BUN risk, accounting for 4.5% of the UA-CRF relationship and 14.4% of the UA-BUN association. Conclusion: In hyperuricemia mice, serum amino acids undergo metabolic changes. Genetically predicted UA levels are positively linked to CRF and BUN, but negatively linked to eGFRcys and eGFRcrea. Ala mediates UA's effect on CRF and BUN risk, indicating Ala could be a target for preventing renal diseases caused by hyperuricemia.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00726-025-03444-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
L-tyrosine inhibits the formation of amyloid fibers of human lysozyme at physiological pH and temperature
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-16 DOI: 10.1007/s00726-025-03445-6
Santos López, Arturo Rojo-Domínguez, Roxana López-Simeon, Alejandro Sosa-Peinado, Hugo Nájera

Amyloid fibers are implicated in numerous diseases, making their study crucial for identifying effective therapeutic compounds. This research highlights the ability of L-tyrosine to inhibit the formation of amyloid fibers in human lysozyme. At a 1:1 molar ratio under physiological conditions (pH 7.4, 37 °C), L-tyrosine significantly reduces amyloid fiber formation, as evidenced by a decrease in thioflavin T fluorescence. Differential scanning calorimetry (DSC) shows a major energy requirement for temperature denaturation when the lysozyme is in the presence of L-tyrosine. Additionally, chemical denaturation experiments reveal a shift in the intrinsic fluorescence spectrum of lysozyme in the presence of L-tyrosine, indicating a direct interaction. Computational docking studies with Molecular Operating Environment (MOE) further confirm that L-tyrosine binds effectively, exhibiting similar binding energies to those of the natural substrate. This study underscores L-tyrosine’s potential as a strong inhibitor of amyloid fiber formation, demonstrating its stabilizing effect on lysozyme and its promise in therapeutic applications.

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引用次数: 0
Metabolism of arginine in juvenile largemouth bass (Micropterus salmoides) after oral or intraperitoneal administration of arginine or its substrates
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-02-13 DOI: 10.1007/s00726-024-03436-z
Jiteng Wang, Jingyi Zhang, Xinyu Li, Hanying Y. Xu, Yeshun Yang, Jiankun Zhang, Wenping Feng, Qiang Chen, Fen Dong, Tao Han

The main objective of this experiment was to study the metabolism of arginine in juvenile largemouth bass (Micropterus salmoides). A total of 300 healthy fish (average weight of 25 ± 0.5 g) were randomly assigned to ten groups. Experimental fish were orally administered or intraperitoneally injected with 0.9% sodium chloride, arginine, arginine-aspartate, citrulline, and glutamate solutions, respectively. They were euthanized at 10, 30, 60, 120, and 240 min after oral administration or intraperitoneal injection, and various tissue samples were subsequently collected for analysis. The results revealed that serum ornithine and citrulline concentrations of largemouth bass were significantly increased by oral administration of arginine or arginine-aspartate (P < 0.05). Intraperitoneal injection of arginine or arginine-aspartate solution significantly elevated the concentrations of ornithine and citrulline in the serum, liver, kidney, and muscles (P < 0.05). The concentrations of citrulline, ornithine, and arginine in serum and muscle increased significantly at 4 h after intraperitoneal injection of glutamate (P < 0.05). Intraperitoneal injection of citrulline significantly increased the concentrations of ornithine and arginine in the serum and muscles (P < 0.05). The research findings demonstrate that both free and small peptide forms of arginine were rapidly degraded to ornithine due to the high arginase activity in various tissues of largemouth bass. Additionally, the pathway of synthesizing citrulline from glutamate and then arginine from citrulline may exist in largemouth bass, but the exact location of this synthesis process may differ from that found in mammals.

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引用次数: 0
Amino acid stable carbon isotopes in nail keratin illuminate breastfeeding and weaning practices of mother – infant dyads
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-30 DOI: 10.1007/s00726-024-03425-2
Hana Salahuddin, Andrea L. Waters-Rist, Fred J. Longstaffe

Compound-specific stable carbon isotope analysis of amino acids (CSIA-AA) is widely used in ecological studies to analyze food-webs and is gaining use in archaeology for investigating past diets. However, its use in reconstructing breastfeeding and weaning practices is not fully understood. This study evaluates the efficacy of stable carbon isotope analysis of amino acids in early life diet reconstruction by analyzing keratin from fingernail samples of three mother-infant pairs during late gestation and early postpartum periods. Our results show that stable carbon isotope ratios (δ13C) of glycine, and to a lesser extent glutamate, effectively trace the onset of exclusive breastfeeding and the end of weaning in infants. We propose that glycine’s ‘conditionally essential’ metabolic pathway during infancy allows it to reflect maternal glycine δ13C, indicating breastmilk consumption. Subtle changes in glutamate δ13C likely result from its ‘non-essential’ status. Additionally, δ13C values of glycine and glutamate indicate maternal physiological and pathological stress due to catabolic effects such as gluconeogenesis. These findings have significant implications for ecological and archaeological research using CSIA-AA for dietary reconstructions. They highlight the need to understand how metabolic pathways affecting δ13C of amino acids may change over an individual’s lifespan or be altered due to various forms of stress.

{"title":"Amino acid stable carbon isotopes in nail keratin illuminate breastfeeding and weaning practices of mother – infant dyads","authors":"Hana Salahuddin,&nbsp;Andrea L. Waters-Rist,&nbsp;Fred J. Longstaffe","doi":"10.1007/s00726-024-03425-2","DOIUrl":"10.1007/s00726-024-03425-2","url":null,"abstract":"<div><p>Compound-specific stable carbon isotope analysis of amino acids (CSIA-AA) is widely used in ecological studies to analyze food-webs and is gaining use in archaeology for investigating past diets. However, its use in reconstructing breastfeeding and weaning practices is not fully understood. This study evaluates the efficacy of stable carbon isotope analysis of amino acids in early life diet reconstruction by analyzing keratin from fingernail samples of three mother-infant pairs during late gestation and early postpartum periods. Our results show that stable carbon isotope ratios (<i>δ</i><sup>13</sup>C) of glycine, and to a lesser extent glutamate, effectively trace the onset of exclusive breastfeeding and the end of weaning in infants. We propose that glycine’s ‘conditionally essential’ metabolic pathway during infancy allows it to reflect maternal glycine <i>δ</i><sup>13</sup>C, indicating breastmilk consumption. Subtle changes in glutamate <i>δ</i><sup>13</sup>C likely result from its ‘non-essential’ status. Additionally, <i>δ</i><sup>13</sup>C values of glycine and glutamate indicate maternal physiological and pathological stress due to catabolic effects such as gluconeogenesis. These findings have significant implications for ecological and archaeological research using CSIA-AA for dietary reconstructions. They highlight the need to understand how metabolic pathways affecting <i>δ</i><sup>13</sup>C of amino acids may change over an individual’s lifespan or be altered due to various forms of stress.</p></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782432/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143063273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Targeted delivery of curcumin and CM11 peptide against hepatocellular carcinoma cells based on binding affinity of PreS1-coated chitosan nanoparticles to SB3 protein
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-25 DOI: 10.1007/s00726-024-03438-x
Danial Rahmani, Ramezan Ali Taheri, Mehrdad Moosazadeh Moghaddam

In recent years, the use of cationic peptides as alternative drugs with anticancer activity has received attention. In this study, the targeted release of curcumin (Cur) and CM11 peptide alone and together against hepatocellular carcinoma (HCC) was evaluated using chitosan nanoparticles (CS NPs) coated with Pres1 that target the SB3 antigen of HCC cells (PreS1-Cur-CM11-CS NPs). SB3 protein is the specific antigen of HCC and the PreS1 peptide is a part of the hepatitis B antigen, which can specifically bind to the SB3 protein. Chitosan was used to prepare NPs. To Cur and CM11 loading, drugs were added to the CS solution in appropriate concentrations. Pres1 was coupled to the surface of the NPs using EDC catalyst to target NPs against HepG2 cells. SEM and DLS analysis confirmed that the PreS1-Cur-CM11-CS NPs had a size of about 132 nm, the ideal size for penetrating the cell membrane. The loading of Cur and CM11 was equal to 87% and 65%, respectively, which had a sustained and better release in the acidic environment than in the physiological environment. The MTT assay showed that PreS1-Cur-CM11-CS NPs act in a targeted and specific manner with the highest toxicity on the HepG2 cells compared to the control by a decrease in viability of about 26% after 48 h based on cell apoptosis. The results showed that PreS1-Cur-CM11-CS NPs are capable of targeted and specific drug release against HepG2 cancer cells and have significant potential to fight this cancer.

Graphical abstract

{"title":"Targeted delivery of curcumin and CM11 peptide against hepatocellular carcinoma cells based on binding affinity of PreS1-coated chitosan nanoparticles to SB3 protein","authors":"Danial Rahmani,&nbsp;Ramezan Ali Taheri,&nbsp;Mehrdad Moosazadeh Moghaddam","doi":"10.1007/s00726-024-03438-x","DOIUrl":"10.1007/s00726-024-03438-x","url":null,"abstract":"<div><p>In recent years, the use of cationic peptides as alternative drugs with anticancer activity has received attention. In this study, the targeted release of curcumin (Cur) and CM11 peptide alone and together against hepatocellular carcinoma (HCC) was evaluated using chitosan nanoparticles (CS NPs) coated with Pres1 that target the SB3 antigen of HCC cells (PreS1-Cur-CM11-CS NPs). SB3 protein is the specific antigen of HCC and the PreS1 peptide is a part of the hepatitis B antigen, which can specifically bind to the SB3 protein. Chitosan was used to prepare NPs. To Cur and CM11 loading, drugs were added to the CS solution in appropriate concentrations. Pres1 was coupled to the surface of the NPs using EDC catalyst to target NPs against HepG2 cells. SEM and DLS analysis confirmed that the PreS1-Cur-CM11-CS NPs had a size of about 132 nm, the ideal size for penetrating the cell membrane. The loading of Cur and CM11 was equal to 87% and 65%, respectively, which had a sustained and better release in the acidic environment than in the physiological environment. The MTT assay showed that PreS1-Cur-CM11-CS NPs act in a targeted and specific manner with the highest toxicity on the HepG2 cells compared to the control by a decrease in viability of about 26% after 48 h based on cell apoptosis. The results showed that PreS1-Cur-CM11-CS NPs are capable of targeted and specific drug release against HepG2 cancer cells and have significant potential to fight this cancer.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":7810,"journal":{"name":"Amino Acids","volume":"57 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantum chemical study of molecular properties of small branched-chain amino acids in water 水中小支链氨基酸分子性质的量子化学研究
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-19 DOI: 10.1007/s00726-024-03437-y
Roman Boča, Žofia Rádiková, Juraj Štofko, Beata Vranovičová, Cyril Rajnák

Four aliphatic amino acids—α-aminobutyric acid (AABA), β-aminobutyric acid (BABA), α-aminoisobutyric acid (AAIBA) and β-aminoisobutyric acid (BAIBA) were investigated in water as a solvent by two quantum chemical methods. B3LYP hybrid version of DFT was used for geometry optimization and a full vibrational analysis of neutral molecules, their cations and anions in the canonical and zwitterionic forms (6 forms for each species). Ab initio DLPNO-CCSD(T) method was applied in the geometry pre-optimized by B3LYP. Calculated molecular descriptors involve dipole moment, quadrupole moment, dipole polarizability, energy of zero-point vibration and total entropic term which enter the standard Gibbs energy. In addition, a set of collective electronic and thermodynamic properties associated with redox process were evaluated: ionization energy, electron affinity, chemical hardness, molecular electronegativity, electrophilicity index, absolute oxidation and reduction potentials. A mutual comparison of these structural isomers including γ-aminobutyric acid (GABA) shows high degree of similarity in molecular descriptors. However, cluster analysis of 12 electro neutral, linear and branched amino acids with 2 – 6 carbon atoms discriminates them into five clusters. It is found that the electrophilicity index correlates with the absolute reduction potential along a straight line (24 items). The reduction potential for canonical structure varies between 1.21 V (glycine) and 1.45 V (AABA) whereas for the zwitterionic form it is visibly lower 0.52–1.11 V. The highest absolute reduction potential > 1.43 V is shown by α-amino acids: α-alanine, AABA (homoalanine) and AAIBA having 2-methyl or 2-ethyl functional group. The calculated absolute oxidation potential correlates with the adiabatic ionization energy and can be used as a criterion of the antioxidant capacity. According to thermodynamic data, the SPLET mechanism of the electron-proton coupled transfer is favored over the alternative SET-PT mechanism. This work contributes to the creation of a database of molecular properties of amino acids based on the same method and basis set.

采用两种量子化学方法研究了4种脂肪族氨基酸- α-氨基丁酸(AABA)、β-氨基丁酸(BABA)、α-氨基异丁酸(AAIBA)和β-氨基异丁酸(BAIBA)在水中作为溶剂的性质。使用B3LYP杂化DFT进行几何优化,并对中性分子及其正阴离子和两性离子形式(每种6种形式)进行了完整的振动分析。采用从头算DLPNO-CCSD(T)方法对B3LYP预优化的几何图形进行求解。计算得到的分子描述符包括偶极矩、四极矩、偶极极化率、零点振动能和进入标准吉布斯能的总熵项。此外,还评估了一系列与氧化还原过程相关的电子和热力学性质:电离能、电子亲和性、化学硬度、分子电负性、亲电性指数、绝对氧化和还原电位。包括γ-氨基丁酸(GABA)在内的这些结构异构体的相互比较显示出分子描述符的高度相似性。然而,对12个2 - 6碳原子的电中性、线性和支链氨基酸进行聚类分析,将它们分为5个簇。发现亲电性指数与绝对还原电位呈直线关系(24项)。典型结构的还原电位在1.21 V(甘氨酸)和1.45 V (AABA)之间变化,而两性离子形式的还原电位明显低于0.52-1.11 V。具有2-甲基或2-乙基官能团的α-氨基酸α-丙氨酸、AABA(同丙氨酸)和AAIBA的绝对还原电位最高,为1.43 V。计算得到的绝对氧化电位与绝热电离能相关,可以作为抗氧化能力的判据。根据热力学数据,电子-质子耦合转移的SPLET机制优于其他的SET-PT机制。这项工作有助于建立基于相同方法和基集的氨基酸分子特性数据库。
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引用次数: 0
Alterations of amino acids in older adults with Alzheimer’s Disease and Vascular Dementia 老年阿尔茨海默病和血管性痴呆患者氨基酸的改变
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-18 DOI: 10.1007/s00726-024-03442-1
Xin Ma, Xin-Meng Wang, Guo-Zhang Tang, Yi Wang, Xue‑Chun Liu, Shuai-Deng Wang, Peng Peng, Xiu-Hong Qi, Xin-Ya Qin, Yue‑Ju Wang, Chen-Wei Wang, Jiang-Ning Zhou

Metabolomics provide a promising tool for understanding dementia pathogenesis and identifying novel biomarkers. This study aimed to identify amino acid biomarkers for Alzheimer’s Disease (AD) and Vascular Dementia (VD). By amino acid metabolomics, the concentrations of amino acids were determined in the serum of AD and VD patients as well as age-matched healthy controls. Several differences in the concentration of amino acids were observed in AD patients compared to both healthy controls and VD patients. However, no significant distinction was found between healthy controls and VD patients. Considering comorbidities, cystine levels were higher in AD than in VD among non-diabetic patients, but not in those with diabetes. Notably, creatine, spermidine, cystine, and tyrosine demonstrated favorable results in decision curve analyses and good discriminative performances, suggesting their potential for clinical application. These fundings give novel perspectives of serum amino acids for predicting metabolic pathways in AD and VD pathogenesis.

代谢组学为理解痴呆的发病机制和识别新的生物标志物提供了一个很有前途的工具。本研究旨在鉴定阿尔茨海默病(AD)和血管性痴呆(VD)的氨基酸生物标志物。通过氨基酸代谢组学,测定了AD和VD患者以及年龄匹配的健康对照血清中的氨基酸浓度。与健康对照组和VD患者相比,AD患者的氨基酸浓度存在一些差异。然而,在健康对照组和VD患者之间没有发现显著差异。考虑到合并症,在非糖尿病患者中,AD患者的胱氨酸水平高于VD患者,但在糖尿病患者中则不然。值得注意的是,肌酸、亚精胺、胱氨酸和酪氨酸在决策曲线分析中表现出良好的结果和良好的判别性能,表明它们具有临床应用潜力。这些发现为预测AD和VD发病机制中的代谢途径提供了血清氨基酸的新视角。
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引用次数: 0
Systematic qualitative proteome-wide analysis of lysine malonylation profiling in Platycodon grandiflorus 桔梗赖氨酸丙二酸酰化的系统定性蛋白质组分析。
IF 3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2025-01-15 DOI: 10.1007/s00726-024-03432-3
Qingshan Yang, Shaowei Xu, Weimin Jiang, Fei Meng, Shuting Wang, Zongping Sun, Na Chen, Daiyin Peng, Juan Liu, Shihai Xing

In recent years, it was found that lysine malonylation modification can affect biological metabolism and play an important role in plant life activities. Platycodon grandiflorus, an economic crop and medicinal plant, had no reports on malonylation in the related literature. This study qualitatively introduces lysine malonylation in P. grandiflorus. A total of 888 lysine malonylation-modified proteins in P. grandiflorus were identified, with a total of 1755 modification sites. According to the functional annotation, malonylated proteins were closely related to catalysis, binding, and other reactions. Subcellular localization showed that related proteins were enriched in chloroplasts, cytoplasm, and nuclei, indicating that this modification could regulate various metabolic processes. Motif analysis showed the enrichment of Alanine (A), Cysteine (C), Glycine (G), and Valine (V) amino acids surrounding malonylated lysine residues. Metabolic pathway and protein-protein interaction network analyses suggested these modifications are mainly involved in plant photosynthesis. Moreover, malonylated proteins are also involved in stress and defense responses. This study shows that lysine malonylation can affect a variety of biological processes and metabolic pathways, and the contents are reported for the first time in P. grandiflorus, which can provide important information for further research on P. grandiflorus and lysine malonylation’s role in environment stress, photosynthesis, and secondary metabolites enrichment.

近年来,人们发现赖氨酸丙二醛化修饰可以影响生物代谢,在植物生命活动中发挥重要作用。桔梗是一种经济作物和药用植物,在相关文献中未见丙二醛酰化的报道。本研究定性地介绍了桔梗中赖氨酸丙二酰化作用。共鉴定出桔梗赖氨酸丙二酰化修饰蛋白888个,修饰位点1755个。根据功能注释,丙二酰化蛋白与催化、结合等反应密切相关。亚细胞定位表明,相关蛋白在叶绿体、细胞质和细胞核中富集,表明这种修饰可以调节多种代谢过程。基序分析显示丙二酰化赖氨酸残基周围富集了丙氨酸(A)、半胱氨酸(C)、甘氨酸(G)和缬氨酸(V)氨基酸。代谢途径和蛋白质相互作用网络分析表明,这些修饰主要参与植物光合作用。此外,丙二酰化蛋白还参与应激和防御反应。本研究表明,赖氨酸丙二酰化可以影响多种生物过程和代谢途径,并首次报道了其含量,为进一步研究赖氨酸丙二酰化在桔花中环境胁迫、光合作用和次生代谢产物富集中的作用提供了重要信息。
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引用次数: 0
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Amino Acids
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