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Changes of testis histology parameters and relative expression of TGF-B4 & StAR genes in roosters fed CoQ10 and omega 3 CoQ10和omega - 3饲喂公鸡睾丸组织学参数及TGF-B4和StAR基因相对表达的变化
Pub Date : 2021-06-03 DOI: 10.22059/JAP.2021.314795.623578
Hamidreza Salari, Y. J. Ahangari, Zarbakht Ansari Pirsaraie
در این پژوهش، اثر تغذیه کوآنزیم Q10و اسیدهای چرب امگا 3 بر بافت شناسی و بیان نسبی ژن‌های TGF-B4 , StAR‌ در بیضه خروس‌های نژاد هوبارد بررسی شد. تعداد 48 خروس در سن 50 هفتگی به صورت تصادفی به چهار گروه مساوی تقسیم شدند و جیره پایه به اضافه سطوحی از کوآنزیم Q10 و امگا 3 شامل صفر،30 گرم در کیلوگرم امگا 3 (سالومگا)، 400 میلی‌گرم در کیلوگرم کوآنزیم Q10 و مخلوط کوآنزیم Q10 و امگا 3 (30+400) در روز به مدت هشت هفته متوالی دریافت کردند. در پایان آزمایش خروس‌ها کشتار و بیضه‌ها به دقت خارج شدند. سپس دو نمونه از یک بیضه از هر تکرار برداشته شد، یک نمونه برای بررسی بافت شناسی به درون محلول فرمالین 10 درصد و یک نمونه جهت بررسی بیان نسبی ژن به درون ازت مایع منتقل شد. نتایج نشان داد که قطر لوله‌های اسپرم ساز در گروه‌های آزمایشی نسبت به گروه شاهد به طور معنی‌داری افزایش یافت ( p<0.05 ) ولی تعداد سلول‌های سرتولی و لایدیگ تفاوت معنی‌داری نداشت. بیان نسبی ژن TGF-B4 در سه گروه آزمایش نسبت به گروه شاهد کاهش یافت ( p<0.05 ). اما بیان نسبی ژن StAR در پرندگانی که بااستفاده از مخلوطی از کوآنزیم Q10 و امگا 3 تغذیه شدند، به طور معنی‌داری افزایش یافت ( p<0.05 ). براساس نتایج این مطالعه اضافه نمودن مخلوطی از کوآنزیم Q10 و اسید های چرب امگا 3 در خوراک خروس‌ها، فراسنجه‌های بافت شناسی و بیان ژن های مرتیط با اسپرم سازی را بهبود بخشید.
在这项研究中,通过Hubbard种族熊顶部TGF-B4基因与StAR的比例,研究了Q10和Amga脂肪酸的影响。50岁时的兔子数量除以四个相等的组,包括0、30克每公斤amma 3、400毫克每公斤Q10和辅酶Q10和amma 3。(30+400)平均每天接受8周的治疗。在兔子试验结束时,兔子被杀死。然后重复两个样本,每个样本的一部分,一个用于将组织测试到10%的探针区域中,另一个用于测试相关基因的样本进入水中。结果显示,试验组的喷雾管道比对照组显著增加(p<0.05),但冰箱和载玻片中的细胞数量没有差异。与对照组相比,三个试验组的TGF-B4比值下降(p<0.05)。但Q10和Amga3中StAR基因与鸟类的比例增加(p<0.05)。因此,本研究改善了Q10和Agga 3的成分
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引用次数: 0
Effect of different level of Tryptophan and methyl group donors on the performance of Japanese quail fed diets contaminated with aflatoxin B1 不同水平的色氨酸和甲基供体对黄曲霉毒素B1污染日本鹌鹑生产性能的影响
Pub Date : 2021-05-29 DOI: 10.22059/JAP.2021.317200.623589
Vahideh Sabaghi-Darmiyan, M. Mehri, F. B. Kasmani, M. Rokouei
The experiment was carried out to investigate the effect of tryptophan, melatonin and dimethylglycine on the growth performance and meat quality of Japanese quails fed diets contaminated with aflatoxin B1 toxin. In this experiment, 680 Japanese quail from 7 to 35 d of age was allotted to 17 treatments and 4 replicates with 10 Japanese quail in each were performed using a central composite design. The performance of birds was calculated on a weekly basis and at the end of the experiment; the amount of Malondialdehyde in meat was examined. the results showed that tryptophan increase weight gain (P<0.05) and melatonin had an increased effect on feed intake (P<0.05). The effect of tryptophan and as well as the interaction of tryptophan and dimethylglycine reduced the amount of malondialdehyde in meat (P<0.05). The results obtained from this research states that dietary supplementation with Tryptophan and methyl group donors have good antioxidant capacity to reduce aflatoxin B1 poisoning on growth performance and Malondialdehyde content of meat, and the use of these supplements in the diet of Japanese quail seems desirable.
本试验旨在研究色氨酸、褪黑素和二甲基甘氨酸对黄曲霉毒素B1污染日粮中日本鹌鹑生长性能和肉品质的影响。试验选用7 ~ 35日龄日本鹌鹑680只,采用中心复合设计,分为17个处理,4个重复,每个重复10只。每周和试验结束时计算雏鸟的生产性能;检测了肉中丙二醛的含量。结果表明,色氨酸提高了增重(P<0.05),褪黑素提高了采食量(P<0.05)。色氨酸的作用以及色氨酸与二甲基甘氨酸的相互作用降低了肉中丙二醛的含量(P<0.05)。本研究结果表明,饲粮中添加色氨酸和甲基供体具有良好的抗氧化能力,可减少黄曲霉毒素B1中毒对肉品生长性能和丙二醛含量的影响,在日本鹌鹑日粮中添加这些补品是可取的。
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引用次数: 0
Comparison of two protocols of estrus synchronization in summer and winter seasons on fertility and blood parameters of Holstein dairy cows 夏季和冬季两种同期发情方案对荷斯坦奶牛生育能力和血液参数的影响
Pub Date : 2021-05-26 DOI: 10.22059/JAP.2021.319102.623594
V. Vahedi, Babak Naderi, Ciyamak Ghazaie, A. Boustan
The aim of this study was the comparison of two protocols of estrus synchronization in two natural climates and heat stress condition on reproductive performance and concentration of plasma estrogen and progesterone hormones in Holstein dairy cows. 110 multiparous Holstein dairy cows (28 kg average daily milk yield and parity one to four calved) were randomly assigned to two protocol groups: 1) Double-Ovsynch (DO; n=60), the cows received GnRH-7d-PGF2α-2d-GnRH and Ovsynch (GnRH-7d- PGF2α-56h-GnRH-16h-AI) was initiated 7 d later; 2) Presynch-Ovsynch (PO; n = 50), the cows received PGF2α-14d-PGF2α and Ovsynch was initiated 12 d later. The both of the protocols were done in two seasons of summer and winter. The calving rate in cows that were synchronized at winter season was greater than cows synchronized in summer (36.4 vs 18.2%; P= 0.04). Mean of the number of insemination to conception was lower in cows that synchronized in winter compared to cows synchronized in summer (1.46 vs 1.74; P= 0.02). Mean of measured estrogen and progesterone hormones concentration was higher in cows that synchronized in winter compared to summer (P < 0.05). Overall, mean of reproductive traits and hormones concentration is higher in cows that synchronized in winter than in summer and the protocol of estrus synchronization had no effect on reproductive performance.
本研究的目的是比较两种自然气候和热应激条件下发情同步的两种方案对荷斯坦奶牛繁殖性能和血浆雌孕激素浓度的影响。110头多胎荷斯坦奶牛(平均日产奶量28kg,产一至四胎)被随机分配到两个方案组:1)双卵同步(DO;n=60),奶牛接受GnRH-7d-PGF2α-2d-GnRH,7天后开始同步(GnRH-7d-PGF2α-56h-GnRH-16h-AI);2) 同步排卵前(PO;n=50),奶牛接受PGF2α-14d-PGF2α,12天后开始同步排卵。这两个方案都是在夏季和冬季两个季节完成的。在冬季同步的奶牛的产仔率高于夏季同步的奶牛(36.4%对18.2%;P=0.04)。与夏季同步的牛相比,冬季同步的牛从受精到受孕的平均次数较低(1.46对1.74;P=0.02)。同步的奶牛测量的雌孕激素浓度的平均值较高总体而言,在冬季同步的奶牛的繁殖性状和激素浓度的平均值高于夏季,发情同步方案对繁殖性能没有影响。
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引用次数: 0
Response of Egg Number to Selection of Different Genotypes of 24-bp Insertion-Deletion Locus in the Promoter of Prolactin Gene of Papua Local Chickens 巴布亚地方鸡催乳素基因启动子24bp插入缺失位点不同基因型选择对蛋数的影响
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.75
Muhammad Mu'in, S. Lumatauw
This study aims to estimate the response of the number of eggs produced up to 240 days of age (EN240) to the selection of Papua local chickens (PLC) with different genotypes from the 24-bp Indel cPRLp locus. A total of 68 PLCs were taken randomly from several breeders in Manokwari as the basic population for selection (G0). Genotype identification of the 24-bp Indel cPRLp locus was performed G0. Based on the distribution of these genotypes, mating pairs were randomly formed G0-II, G0-DD and G0-ID to produce G1-II, G1-DD, and G1-Control (G0-ID offspring). Heritability of EN240 in G1-II and G1-DD populations were estimated in full-sib (single pairs mating). Individual selection based on breeding value EN240 was carried out on G1-II (♀) and G1-DD (♀) to form the selection generation (GS): GS-II (♀) and GS-DD (♀). Selection was also carried out on G1II (♂) and G1-DD (♂) based on body weight at 240 days (BW240) to become selected GS-II (♀) and GS-DD (♀) mating partners. GS-Control was also formed through 25% random sampling from G1-Control (♂ and ♀). GS mating resulted in the second generation (G2): G2-II, G2-DD, and G2-Control. Age at first laying (AFL), EN240, and mean egg weight (EWA) in hens of G1, GS, and G2 were recorded. Response to selection for EN240 was calculated by two methods predicted selection response (Rp) and actual selection response (Rr). Both methods of calculation yield positive and high values. In actual response (Rr), PLC in II genotypes group are more responsive to the selection treatment than DD genotype group. Selection increase EN240 impact accelerate of AFL and lower the EWA, because of their negative genetic correlation.
本研究旨在估计从24bp Indel-cPRLp基因座中选择具有不同基因型的巴布亚土鸡(PLC)时,240日龄以下产蛋数量(EN240)的反应。从马诺夸里的几个育种家中随机抽取了68个PLC作为基本群体进行选择(G0)。对24 bp Indel cPRLp基因座进行G0基因型鉴定。根据这些基因型的分布,随机形成G0-II、G0-DD和G0-ID交配对,产生G1-II、G1-DD和G1对照(G0-ID后代)。EN240在G1-II和G1-DD群体中的遗传力是在全同胞(单对交配)中估计的。在G1-II上进行了基于育种值EN240的个体选择(♀) 和G1-DD(♀) 以形成选择生成(GS):GS-II(♀) 和GS-DD(♀). G1II也进行了选择(♂) 和G1-DD(♂) 基于240天时的体重(BW240)成为选定的GS-II(♀) 和GS-DD(♀) 交配伴侣。GS对照组也是通过G1对照组25%的随机抽样形成的(♂ 和♀). GS交配产生了第二代(G2):G2-II、G2-DD和G2对照。记录了G1、GS和G2母鸡的初产年龄(AFL)、EN240和平均蛋重(EWA)。通过预测选择响应(Rp)和实际选择响应(Rr)两种方法计算EN240的选择响应。这两种计算方法都产生了正值和高值。在实际反应(Rr)中,II基因型组的PLC比DD基因型组对选择处理更有反应。选择增加EN240影响AFL的加速和EWA的降低,因为它们之间存在负遗传相关性。
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引用次数: 0
The Effect of Supplementation of Avocado Seed Flour (Persea americana Mill.) in Feed on Blood Lipids Profile and Egg Yolk Cholesterol of Japanese Quail (Corturnix-corturnix japonica) 饲料中添加鳄梨籽粉对日本鹌鹑(Corturnix Corturnix japonica)血脂和蛋黄胆固醇的影响
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.28
Yosua Sujud Apriyanto, N. Iriyanti, E. Tugiyanti
The purpose of this research is to determine the effect of avocado seed flour (ASF) supplementation in feed on blood lipids and quail egg yolk cholesterol. The materials were 100 female quails aged 4 weeks old with ration ingredients consist of corn, bran, paddy, soybean meal, fish flour, avocado seed flour, palm oil, CaCO3, premix, lysine, and methionine. The research conducted an experiment with 4 treatments of ASF supplementation levels incorporated into basal feed, namely 0% ASF (R0), 3% ASF (R1), 6% ASF (R2), and 9% ASF (R3). The observed variables included blood cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL), triglyceride, and egg yolk cholesterol. The data were subjected to the one-way Analysis of variance (ANOVA) in a completely randomized design, followed by HSD test when differences between treatments were observed. The result showed that ASF supplementation significantly reduced blood cholesterol levels and the egg yolk cholesterol, did not significantly affect LDL and triglyceride levels. The HDL levels and egg yolk cholesterol tend to decrease as the level of ASF increased. It is concluded that avocado seed flour (ASF) supplementation up to 9% is safe for quail feed supplementation.
本研究的目的是确定在饲料中添加鳄梨籽粉(ASF)对血脂和鹌鹑蛋黄胆固醇的影响。材料为100只4周龄的雌性鹌鹑,日粮成分包括玉米、麸皮、稻谷、豆粕、鱼粉、鳄梨籽粉、棕榈油、CaCO3、预混料、赖氨酸和蛋氨酸。本研究在基础饲料中加入了4种补充ASF水平的处理,即0%ASF(R0)、3%ASF(R1)、6%ASF(R2)和9%ASF(R3)。观察到的变量包括血液胆固醇、高密度脂蛋白(HDL)、低密度脂蛋白、甘油三酯和蛋黄胆固醇。在完全随机设计中对数据进行单因素方差分析(ANOVA),然后在观察到治疗之间的差异时进行HSD检验。结果表明,补充ASF可显著降低血液胆固醇和蛋黄胆固醇水平,对LDL和甘油三酯水平没有显著影响。高密度脂蛋白水平和蛋黄胆固醇往往会随着ASF水平的升高而降低。得出的结论是,添加高达9%的鳄梨籽粉(ASF)对鹌鹑饲料是安全的。
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引用次数: 0
Identification of Goats’ and Cows’ Milk Protein Profile in Banyumas Regency by Sodium Dedocyl Sulphate Gel Electrophoresis (Sds-Page) 十二烷基硫酸钠凝胶电泳(Sds-Page)测定山羊和奶牛乳蛋白谱
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.37
H. S. Widodo, T. Y. Astuti, Pramono Soediarto, A. N. Syamsi
Protein is one of the nutrient components in milk that is related to product quality. The components of milk protein are divided into casein alpha-s1, beta, alpha-s2, kappa, and whey fractions such as alpha lactalbumin and beta lactoglobulin. There are no existing data of milk protein fraction in dairy cow and goats in Banyumas Regency. This study aimed to determine the profile in form of protein fractions of cow and goat milk in Banyumas. Milk sample from fifty cows and thirty dairy goats was taken by random sampling in some areas. The milk protein profile was identified by the technique of sodium dodecyl sulphate gel electrophoresis (SDSPAGE) and protein quantity prediction by software. The data obtained were analyzed statistically by MannWhitney between cows and goats. The results were significantly different (p<0.05) between cows and goats in molecular weight of protein alpha-S1 casein (29.66 vs 33.37 kDa), alpha-S2 (27.76 vs 29.49 kDa), beta (24 , 48 vs 25.59 kDa) and beta lactoglobulin (15.75 vs 15.97 kDa). The quantity of casein alpha-S1 (7.88 vs 4.16 g/l), alpha-S2 (1.31 vs. 4.02 g/l), beta (8.74 vs 14.24 g/l), kappa (2.41 vs. 4.28 g/l) and alpha lactalbumin (0.91 vs 0.7 g / l) was significantly different (p <0.05) between cow's and goat's milk, respectively. In conclusion, milk protein profile of cows and goats in Banyumas Regency is different.
蛋白质是牛奶中与产品质量有关的营养成分之一。乳蛋白的成分分为酪蛋白α-s1、β、α-s2、κ和乳清组分,如α-乳清蛋白和β-乳球蛋白。Banyumas Regency的奶牛和山羊的乳蛋白含量没有现有数据。本研究旨在确定香蕉中牛奶和羊奶蛋白质组分的形态特征。在一些地区,对50头奶牛和30只奶山羊进行了随机抽样。采用十二烷基硫酸钠凝胶电泳(SDSPAGE)技术对牛奶蛋白质进行了鉴定,并用软件对蛋白质含量进行了预测。MannWhitney对获得的数据在奶牛和山羊之间进行了统计分析。牛和山羊的蛋白质α-S1酪蛋白(29.66 vs 33.37kDa)、α-S2(27.76 vs 29.49kDa),β(24,48 vs 25.59kDa)和β乳球蛋白(15.75 vs 15.97kDa。酪蛋白α-S1(7.88 vs.4.16 g/l)、α-S2(1.31 vs.4.02 g/l)、β(8.74 vs.14.24 g/l)、κ(2.41 vs.4.28 g/l)和α-乳清蛋白(0.91 vs.0.7 g/l)的含量在牛奶和山羊奶之间分别存在显著差异(p<0.05)。总之,Banyumas Regency的奶牛和山羊的乳蛋白图谱是不同的。
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引用次数: 3
Estrous Performance of Etawah Crossbred Goats Following Different Estrous Synchronization Methods Etawah杂交山羊不同同期发情方法的发情性能
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.74
N. Solihati, S. Rasad, K. Winangun, Toha Toha
The objective of the research was to determine the estrous profile of Etawah Crossbred goats after estrous synchronization with different methods. Eighteen does aged 12-24 month old were divided in three groups to receive estrous synchronization treatments (T1 = 14 days intravaginal implant of 60 mg of progesterone (MPA), T2 = two times injection of 5 mg PGF2α (lutalyse) in 11 days interval, and T3 = 10 days of intravaginal implant of 60 mg of progesterone (MPA) + injection of 5 mg PGF2α 48 hours before removal) with six replications. The parameters consisted of estrous behaviour, changes in size and colour of vulva, and duration of estrus when the number of superficial and keratin cells were dominating in the vaginal mucus cell. Data from estrous behaviour and score of vulvar colour was analyzed using Kurkal Wallis test, while onset of estrus, size of vulva slit and estrous duration was analyzed using ANOVA and Duncan test. The result showed that estrous behaviour and changes in color and size of vulva were not significantly different, but estrous duration was significantly different. Estrous duration in T1 (31.30 hour) and T2 (31.10 hour) was significantly longer than that of T3 (11.36 hour). It is concluded that different methods of estrus synchronization affected estrous quality equally but it affected the estrous duration differently based on vaginal mucus cells. Treatment implant vaginal sponge content progesterone for 14 days and double injection of PGF2α with 11-day interval given longest estrous duration.
本研究的目的是用不同的方法测定Etawah杂交山羊发情同步后的发情情况。将年龄在12-24个月大的18只雌二醇分为三组,接受雌激素同步治疗(T1=14天阴道内植入60 mg孕酮(MPA),T2=11天间隔两次注射5 mg PGF2α(lutalyse),T3=10天阴道内种植60 mg孕酮+在去除前48小时注射5 mg前列腺素F2α),共6次重复。这些参数包括发情行为、外阴大小和颜色的变化,以及阴道粘液细胞中浅表细胞和角蛋白细胞占主导地位时的发情持续时间。使用Kurkal-Wallis检验分析发情行为和外阴颜色评分的数据,同时使用ANOVA和Duncan检验分析发情开始、外阴切口大小和发情持续时间。结果表明,发情行为及外阴颜色和大小的变化没有显著差异,但发情持续时间有显著差异。T1(31.30小时)和T2(31.10小时)的发情期明显长于T3(11.36小时)。结果表明,不同的同期发情方法对发情质量的影响相同,但对发情持续时间的影响因阴道粘液细胞而异。治疗植入阴道海绵内容物孕酮14天,间隔11天双重注射PGF2α,给予最长的发情期。
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引用次数: 2
The Prolific Variation, Body Morphometrics, and Breeding Value of Indonesian Local Etawah Goat Based in East Java 东爪哇印尼当地Etawah山羊的繁殖变异、体型和育种价值
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.85
M. Mudawamah, G. Ciptadi, Irawati Dinasari Retnaningtyas
A crucial trait of a high economic value of goats is calving to more than one kid (prolificacy potency). The high prolificacy potency (> 1 kid) has a higher income compared to single kids. This study described the potential of Indonesian Local Etawah Goat (ILEG) for prolific trait and the morphometric of body and breeding values in various environments as a basis for selection.  It involved smallholder farmers who breed ILEG does from 14 villages in East Java. The research was conducted on a field survey to obtain primary data about the phenotypic superior ILEG goats based on the status of the prolific trait. The study used 520 does with 1347 prolific records obtained. The results showed that the prolificacy values ranged from 2.12-1.42 heads/calving (medium to high category). The variation of prolificacy was 0.53, and the breeding values of the prolificacy trait were 1.48-1.74. The average of body morphometrics was varied with the following details.  Chest circumference was 81.06 + 4.63 cm, body length was 76.64 + 4.33 cm, shoulder height was 75.34 + 5.83 cm and ear length were 27.44 + 3.02 cm.  This study concluded that the prolific rate was medium to high category.  The prolific variation was higher than body morphometry variation, and the prolificacy EBVs of breeding villages divided into four unique pattern boxplots. The prolific trait could be the basis for new considerations in the ILEG breeding program, either through selection or mating.
山羊高经济价值的一个关键特征是产下多个孩子(多产能力)。与独生子女相比,高繁殖力(>1个孩子)的收入更高。本研究描述了印尼当地埃塔瓦山羊(ILEG)在各种环境中的多产性状潜力、身体形态计量学和育种价值,作为选择的基础。它涉及来自东爪哇14个村庄的养殖ILEG的小农户。本研究是在实地调查的基础上,根据高产性状的状况,获得表型优越的ILEG山羊的初步数据。这项研究使用了520个干细胞,获得了1347个高产记录。结果表明,繁殖力值在2.12-1.42头/产仔(中高类)之间。繁殖力变异为0.53,繁殖力性状的育种值为1.48-1.74。身体形态计量学的平均值随以下细节而变化。胸围81.06+4.63cm,体长76.64+4.33cm,肩高75.34+5.83cm,耳长27.44+3.02cm。繁殖村的繁殖力EBV分为四个独特的模式箱区。高产性状可能是ILEG育种计划中新考虑的基础,无论是通过选择还是交配。
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引用次数: 4
Developing Beef Cattle in Banyumas Regency: Potentials and Strategies 在巴尤马斯县发展肉牛:潜力和策略
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.4
N. N. Hidayat, K. Muatip, R. Widiyanti
Optimum development of beef cattle is feasible in the potential area with effective strategies. This research was conducted in Banyumas regency. The purpose of this study was to describe the potential of Banyumas Regency in developing beef cattle farming as the basis for its development strategy. A survey method was conducted to obtain the secondary data supported by primary data subjected to simple descriptive statistical analysis consisting of mean values, distribution frequency, cross-tabulation and trend analysis. Ruminant potentials were investigated using LQ analysis, and developmental strategies were formulated using a SWOT analysis. The result showed a fluctuated but positive improvement trend of beef cattle population in Banyumas. Sub-districts potentially developed for beef cattle centres (LQ>1) included Kembaran, Sokaraja, Kalibagor, Kedungbanteng, Karanglewas, Sumbang and Baturraden. A quantitative SWOT matrix analysis showed that the internal factor was 0.0298 (X-axis), and the external factor was – 0.2941 (Y-axis). Conclusively, The key strategy to develop beef cattle in Banyumas was a differential strategy.
有了有效的对策,潜力区肉牛优化发展是可行的。这项研究是在Banyumas摄政时期进行的。本研究的目的是描述Banyumas Regency在发展肉牛养殖方面的潜力,作为其发展战略的基础。采用问卷调查的方法,通过均值、分布频率、交叉表和趋势分析等简单的描述性统计分析,获得有一手数据支持的二次数据。利用LQ分析法对反刍动物潜力进行调查,并利用SWOT分析法制定发展策略。结果表明,Banyumas肉牛种群呈波动但正向的改善趋势。可能发展为肉牛中心的街道包括Kembaran、Sokaraja、Kalibagor、Kedungbanteng、Karanglewas、Sumbang和Baturraden。定量SWOT矩阵分析表明,内部因素为0.0298 (x轴),外部因素为- 0.2941 (y轴)。综上所述,Banyumas地区发展肉牛的关键策略是差异化策略。
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引用次数: 2
Egg Quality from Avian Species: Electrophoretic Characterization of Egg White Proteins 禽类的蛋品质:蛋清蛋白的电泳特性
Pub Date : 2021-03-31 DOI: 10.20884/1.JAP.2021.23.1.2
Meziani Samira, D. El-Mokhtar, Menadi Noureddine, D. Mustapha, Z. Mohammed, Bouterfes Mohammed, Benali Mohammed
The aim of the present study was to evaluate and to compare the physicochemical composition of egg white, and to characterize and estimate the quantitative variations of egg white proteins of different birds’ species (duck, quail, Chicken, partridge and goose). For each bird species, the whole egg temperature, weight, height, density and shape index were evaluated. After breaking the eggs, a visual examination was performed then vitelline (VI) and albumen (AI) indexes and the haugh unit (HU) were measured. The study of the quality and the characterization of egg white proteins were performed by Agarose Gel Electrophoresis. The total egg weight varied within a range of (8.98±0.82 g to 142.91±10.34 g) for quail and goose, respectively. The thickness of the egg yolk was almost three times higher than that of the egg white in all breeds. The egg freshness, measured by HU, was higher in partridge, followed by quail and chicken breeds with values of 88.81±0.09, 87.45±3.33, 81.53±2.16, respectively. The VI shows values of about 0.40. The egg yolk/ white ratio ranged from 47 and the pH varied from 8.37 to 8.95 for all local species studied. The egg white protein content was higher for all species (up to 14.45g/100 g). Three types of proteins are identified by agarose gel electrophoresis (ovalhibitor, ovotransferrin and ovalbumin) with a quantitative difference between bird species. Densitometric analysis shows a qualitative difference due to the presence of type C lysozyme in chicken egg white.
本研究的目的是评估和比较蛋清的理化组成,并表征和估计不同鸟类(鸭、鹌鹑、鸡、鹧鸪和鹅)蛋清蛋白的定量变化。对每种鸟类的全蛋温度、重量、高度、密度和形状指数进行了评估。打碎鸡蛋后,进行目视检查,然后测量卵黄(VI)和白蛋白(AI)指数以及高单位(HU)。采用琼脂凝胶电泳法对蛋清蛋白的质量和性质进行了研究。鹌鹑和鹅的总蛋重变化范围分别为(8.98±0.82g至142.91±10.34g)。在所有品种中,蛋黄的厚度几乎是蛋清的三倍。用HU测定的鸡蛋新鲜度,鹧鸪较高,其次是鹌鹑和鸡,分别为88.81±0.09、87.45±3.33和81.53±2.16。VI显示的值约为0.40。研究的所有当地物种的蛋黄/蛋清比例在47之间,pH值在8.37至8.95之间。所有物种的蛋清蛋白含量均较高(高达14.45g/100g)。通过琼脂糖凝胶电泳鉴定了三种类型的蛋白质(卵清蛋白、卵转铁蛋白和卵清蛋白),不同鸟类之间存在定量差异。密度分析显示,由于蛋清中存在C型溶菌酶,因此存在定性差异。
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Animal production
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