Pub Date : 2021-03-31DOI: 10.20884/1.JAP.2021.23.1.88
R. S. Harahap, R. R. Noor, A. Gunawan
HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fattailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.
{"title":"Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep","authors":"R. S. Harahap, R. R. Noor, A. Gunawan","doi":"10.20884/1.JAP.2021.23.1.88","DOIUrl":"https://doi.org/10.20884/1.JAP.2021.23.1.88","url":null,"abstract":"HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fattailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"1 1","pages":"44-53"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47000738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-20DOI: 10.20884/1.JAP.2020.22.3.77
R. Naufalin, E. Wuryatmo, R. Wicaksono, Laila Sausan El Islami
This study aims to determine the effect of the type and concentration of preservative powder with foam-mat drying method to produce optimal beef meatballs products seen from the chemical, sensory, and antioxidant evaluation. This study used an experimental method with a randomized block design with the factors being studied were the type of powder of kecombrang plant parts, namely flowers and stems with the concentrations used, namely 0%, 1%, 2%, and 3%. Physicochemical analysis was performed on moisture content, pH, and total protein hydrolyzed (Formol test). The sensory evaluation was carried out by 25 trained people using the descriptive and hedonic testing methods on a scale of 1-5 (very dislike to very much like). Meanwhile, the best treatment from the results of physicochemical and sensory evaluation was analyzed of bioactive compounds (antioxidants) was carried out on total flavonoids and total phenols. The results showed that the best treatment combination was the type of flower preservative with a concentration of 2% which had characteristics with an average moisture content of 57.19 ± 5.71%, pH 7.1 ± 0.21, hydrolyzed protein content (Formol) 1.40 ± 0.35%, whitish-gray color (2.72 ± 1.40), scents of meat and kecombrang (3.64 ± 0.49), slightly chewy texture (3.52 ± 0.65), flavored with meat and kecombrang (3.68 ± 0.75), is rather preferenced by panelists (3.12 ± 0.97), and results of antioxidants evaluation were total flavonoids 2.42 mg QE / 100 mg, total phenolic 179.53 mg QE / 100 mg. The concentration of kecombrang flower and stems powder suitable for adding to beef meatballss is seen from the physicochemical and sensory evaluation up to 2%.
{"title":"Sensory, Physicochemical and Antioxidants Evaluation of Kecombrang (Etlingera elatior) Preservative Powder with Foam-mat Drying Method for Beef Meatballs Products","authors":"R. Naufalin, E. Wuryatmo, R. Wicaksono, Laila Sausan El Islami","doi":"10.20884/1.JAP.2020.22.3.77","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.77","url":null,"abstract":"This study aims to determine the effect of the type and concentration of preservative powder with foam-mat drying method to produce optimal beef meatballs products seen from the chemical, sensory, and antioxidant evaluation. This study used an experimental method with a randomized block design with the factors being studied were the type of powder of kecombrang plant parts, namely flowers and stems with the concentrations used, namely 0%, 1%, 2%, and 3%. Physicochemical analysis was performed on moisture content, pH, and total protein hydrolyzed (Formol test). The sensory evaluation was carried out by 25 trained people using the descriptive and hedonic testing methods on a scale of 1-5 (very dislike to very much like). Meanwhile, the best treatment from the results of physicochemical and sensory evaluation was analyzed of bioactive compounds (antioxidants) was carried out on total flavonoids and total phenols. The results showed that the best treatment combination was the type of flower preservative with a concentration of 2% which had characteristics with an average moisture content of 57.19 ± 5.71%, pH 7.1 ± 0.21, hydrolyzed protein content (Formol) 1.40 ± 0.35%, whitish-gray color (2.72 ± 1.40), scents of meat and kecombrang (3.64 ± 0.49), slightly chewy texture (3.52 ± 0.65), flavored with meat and kecombrang (3.68 ± 0.75), is rather preferenced by panelists (3.12 ± 0.97), and results of antioxidants evaluation were total flavonoids 2.42 mg QE / 100 mg, total phenolic 179.53 mg QE / 100 mg. The concentration of kecombrang flower and stems powder suitable for adding to beef meatballss is seen from the physicochemical and sensory evaluation up to 2%.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"163-172"},"PeriodicalIF":0.0,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49277121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-20DOI: 10.20884/1.JAP.2020.22.3.21
Aziz Nur Rahmat, W. Suryapratama, F. Suhartati
The research aimed to examine the effect of red dragon fruit skin and guava leaves supplemented into beef cattle feed on the concentration of partial VFA and methane gas production. The in vitro study was conducted in a completely randomized design (CRD) with 3 treatments, namely P0: 50 % concentrate + 50 % ammoniated straw (Control), P1: 50 % concentrate + 50 % ammoniated straw + 5 % red dragon fruit skin flour, and P2: 50 % concentrate + 50 % ammonia straw + 5 % red dragon fruit skin flour + 2.5 % guava leaf flour. The concentrate consisted of 2 parts of rice bran and 1 part of coconut meal. The ratio of concentrat and ammoniated rice straw was 50:50. Each treatment was repeated 6 times. The results showed that the concentration of acetic acid, butyrate and methane gas decreased while the concentration of propionate increased with the addition of red dragon fruit and guava leaves. The research concluded that the addition of 5 % red dragon fruit skin flour combined with 2.5 % guava leaves flour in beef cattle feed could further reduce the concentration of acetic acid, butyric acid and the production of methane gas, but increase the concentration of propionic acid. It is suggested that defaunated agents of red dragon fruit skin and guava leaves could reduce the production of methane gas in the rumen of beef cattle.
{"title":"Concentration of Partial VFA and Methane Production of Beef Cattle Rument Fluid which Red Dragon Fruit Skin (Hylocereus costaricensis) and Guava Leaf (Psidium guajava L.) in Ammoniated Rice Straw Based Ration","authors":"Aziz Nur Rahmat, W. Suryapratama, F. Suhartati","doi":"10.20884/1.JAP.2020.22.3.21","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.21","url":null,"abstract":"The research aimed to examine the effect of red dragon fruit skin and guava leaves supplemented into beef cattle feed on the concentration of partial VFA and methane gas production. The in vitro study was conducted in a completely randomized design (CRD) with 3 treatments, namely P0: 50 % concentrate + 50 % ammoniated straw (Control), P1: 50 % concentrate + 50 % ammoniated straw + 5 % red dragon fruit skin flour, and P2: 50 % concentrate + 50 % ammonia straw + 5 % red dragon fruit skin flour + 2.5 % guava leaf flour. The concentrate consisted of 2 parts of rice bran and 1 part of coconut meal. The ratio of concentrat and ammoniated rice straw was 50:50. Each treatment was repeated 6 times. The results showed that the concentration of acetic acid, butyrate and methane gas decreased while the concentration of propionate increased with the addition of red dragon fruit and guava leaves. The research concluded that the addition of 5 % red dragon fruit skin flour combined with 2.5 % guava leaves flour in beef cattle feed could further reduce the concentration of acetic acid, butyric acid and the production of methane gas, but increase the concentration of propionic acid. It is suggested that defaunated agents of red dragon fruit skin and guava leaves could reduce the production of methane gas in the rumen of beef cattle.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"173-180"},"PeriodicalIF":0.0,"publicationDate":"2021-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42484476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-12DOI: 10.20884/1.JAP.2020.22.3.69
Restuti Fitria, Siti Rahmawati Zulaikhah, N. Hindratiningrum
This research aimed to evaluate the addition of M21 Decomposer (MD) and urea (U) on the Dry matter digestibility (DMD) and Organic matter digestibility (OMD) in vitro. There were five treatments and five replicates. The treatments in this study were the addition of MD and U at different levels. namely R0 = Corn cob without amofer/control; R1 = Amofer Corn cob plus 0.04% MD+3% U; R2 = Amofer Corn cob plus 0.06% MD+3% U; R3 = Amofer Corn cob plus 0.04% MD+5% U; and R4 = Amofer Corn cob plus 0.06% MD+5% U. The obtained data were subjected to Analysis of Variance and continued by an Orthogonal Contrast. The result showed that the treatments significantly affected (P<0.05) both DMD and OMD digestibility. The digestibility of amofer corncob was higher than the non-amofer that exhibited 17.982±2.4409% DMD and 26.024±3.009% OMD. The highest DMD and OMD digestibility was observed in R4. i.e.. 24.655±4.858% and 34.276±5.176%. respectively. In conclusion. the best level in the incorporating MD and U is at MD 0.06% and U 5% could improve DMD by 6.673% and OMD by 8.252%.
{"title":"Evaluating the Digestibility of Ammonia Fermented (Amofer) Corn Cob Using Different Levels of M21 Decomposer and Urea (In Vitro Study)","authors":"Restuti Fitria, Siti Rahmawati Zulaikhah, N. Hindratiningrum","doi":"10.20884/1.JAP.2020.22.3.69","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.69","url":null,"abstract":"This research aimed to evaluate the addition of M21 Decomposer (MD) and urea (U) on the Dry matter digestibility (DMD) and Organic matter digestibility (OMD) in vitro. There were five treatments and five replicates. The treatments in this study were the addition of MD and U at different levels. namely R0 = Corn cob without amofer/control; R1 = Amofer Corn cob plus 0.04% MD+3% U; R2 = Amofer Corn cob plus 0.06% MD+3% U; R3 = Amofer Corn cob plus 0.04% MD+5% U; and R4 = Amofer Corn cob plus 0.06% MD+5% U. The obtained data were subjected to Analysis of Variance and continued by an Orthogonal Contrast. The result showed that the treatments significantly affected (P<0.05) both DMD and OMD digestibility. The digestibility of amofer corncob was higher than the non-amofer that exhibited 17.982±2.4409% DMD and 26.024±3.009% OMD. The highest DMD and OMD digestibility was observed in R4. i.e.. 24.655±4.858% and 34.276±5.176%. respectively. In conclusion. the best level in the incorporating MD and U is at MD 0.06% and U 5% could improve DMD by 6.673% and OMD by 8.252%.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"154-157"},"PeriodicalIF":0.0,"publicationDate":"2021-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41859051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-12DOI: 10.20884/1.JAP.2020.22.3.67
P. Wulansari, N. Rahayu, N. Frasiska
This study aimed to analyze the antioxidant activities and microbiological and physicochemical characteristics of cow milk kefir fortified with ginger extract (Zingiber officinale). The ginger extract was incorporated together with grain before the fermentation. The levels of ginger extract concentrate were 0, 0.5, 1.0, 1.5, and 2.0 % (w/w). The ginger used in this study is a thick ginger extract that is added before the kefir fermentation process. The result showed that a higher level of ginger extract added to the fermentation could improve the antioxidant activities of the cow milk kefir. The kefir sample fortified with 2.0% ginger extract produced the highest antioxidant activities, particularly DPPH IC50 (0.32%) and a total polyphenol of 0.72%. This study found that the higher the ginger extract level, the lower the total count, total LAB, and total yeast. The average value of physicochemical characteristics for free fatty acid, lactic acids, alcohol, and pH in the present study was 0.5503 mg/KOH, 1.0005%, 1.628 g/dL, and 4.42, respectively. In conclusion, the ginger extract was effective for producing kefir with high antioxidant activities (DPPH IC50) and total polyphenol.
{"title":"The Effect of Incorporating Ginger Extract (Zingiber officinale) to Cow Milk Kefir: An Analysis of Antioxidant and Microbiological and Physicochemical Characteristics","authors":"P. Wulansari, N. Rahayu, N. Frasiska","doi":"10.20884/1.JAP.2020.22.3.67","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.67","url":null,"abstract":"This study aimed to analyze the antioxidant activities and microbiological and physicochemical characteristics of cow milk kefir fortified with ginger extract (Zingiber officinale). The ginger extract was incorporated together with grain before the fermentation. The levels of ginger extract concentrate were 0, 0.5, 1.0, 1.5, and 2.0 % (w/w). The ginger used in this study is a thick ginger extract that is added before the kefir fermentation process. The result showed that a higher level of ginger extract added to the fermentation could improve the antioxidant activities of the cow milk kefir. The kefir sample fortified with 2.0% ginger extract produced the highest antioxidant activities, particularly DPPH IC50 (0.32%) and a total polyphenol of 0.72%. This study found that the higher the ginger extract level, the lower the total count, total LAB, and total yeast. The average value of physicochemical characteristics for free fatty acid, lactic acids, alcohol, and pH in the present study was 0.5503 mg/KOH, 1.0005%, 1.628 g/dL, and 4.42, respectively. In conclusion, the ginger extract was effective for producing kefir with high antioxidant activities (DPPH IC50) and total polyphenol.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"148-153"},"PeriodicalIF":0.0,"publicationDate":"2021-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42469707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-12DOI: 10.20884/1.JAP.2020.22.3.22
Risda Amelia Putri Nasution, S. Rahayu, M. Bata
The interaction of Hibiscus Leaf Meal (HLM) and Direct-Fed Microbials (DFM) supplementation on nitrogen metabolism and rumen microbial synthesis by local sheep was investigated. Thirty six male local sheep aged ±2 years, weight 28.01±2.61 kg were fed concentrate (3% of body weight) supplemented with HLM twice a day, amoniated rice-straw (ARS) supplemented with DFM were given ad-libitum were assigned randomly to nine treatment in an experiment of 3×3 factorial design. The first factor (P) was DFM were supplemented in ARS (P0=without DFM, P1=DFMAMS, and P2=DFMRK). Second factor (W) was the level of HLM supplementation in concentrate (W0=0%, W1=0.24% and W2=0.48% of DM consentrate. The study measured variables including nitrogen digestibility (ND), nitrogen retention (NR), microbial protein synthesis (MPS), and Efficiency of microbial protein synthesis (EMPS). There were significant interaction between DFM and HLM suplementation on MPS and EMPS. However, the interaction of ND and NR was non-existent. Based on polynomial graph, the most efficient MPS was achieved on combination between DFMRK and 0.23% HLM about 8.70 gN/day. Despite the absence of interaction (P>0.05) between DFM supplementation and HLM on KN and RN, DFM supplementation significantly affected (P<0.01) Nitrogen metabolism (ND and NR). The treatment without DFM supplementation resulted the highest nitrogen metabolism (ND and NR). This study concludes that HLM without DFM suplementation positively impacted N Metabolism. Combination between 0.23% HLM and DFMRK resulted the most efficient Microbial Protein Synthesis.
{"title":"Nitrogen Metabolism and Microbial Protein Synthesis by Local Sheep Fed Diet Containing Hibiscus Leave Meal (HLM) with Different Direct-Fed Microbials (DFM) Supplementation","authors":"Risda Amelia Putri Nasution, S. Rahayu, M. Bata","doi":"10.20884/1.JAP.2020.22.3.22","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.22","url":null,"abstract":"The interaction of Hibiscus Leaf Meal (HLM) and Direct-Fed Microbials (DFM) supplementation on nitrogen metabolism and rumen microbial synthesis by local sheep was investigated. Thirty six male local sheep aged ±2 years, weight 28.01±2.61 kg were fed concentrate (3% of body weight) supplemented with HLM twice a day, amoniated rice-straw (ARS) supplemented with DFM were given ad-libitum were assigned randomly to nine treatment in an experiment of 3×3 factorial design. The first factor (P) was DFM were supplemented in ARS (P0=without DFM, P1=DFMAMS, and P2=DFMRK). Second factor (W) was the level of HLM supplementation in concentrate (W0=0%, W1=0.24% and W2=0.48% of DM consentrate. The study measured variables including nitrogen digestibility (ND), nitrogen retention (NR), microbial protein synthesis (MPS), and Efficiency of microbial protein synthesis (EMPS). There were significant interaction between DFM and HLM suplementation on MPS and EMPS. However, the interaction of ND and NR was non-existent. Based on polynomial graph, the most efficient MPS was achieved on combination between DFMRK and 0.23% HLM about 8.70 gN/day. Despite the absence of interaction (P>0.05) between DFM supplementation and HLM on KN and RN, DFM supplementation significantly affected (P<0.01) Nitrogen metabolism (ND and NR). The treatment without DFM supplementation resulted the highest nitrogen metabolism (ND and NR). This study concludes that HLM without DFM suplementation positively impacted N Metabolism. Combination between 0.23% HLM and DFMRK resulted the most efficient Microbial Protein Synthesis.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"137-147"},"PeriodicalIF":0.0,"publicationDate":"2021-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43788737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-12DOI: 10.20884/1.JAP.2020.22.3.64
Nu'man - Hidayat, Chomsiatun Nurul Hidayah, A. P. Nugroho
The research compared the changes motility and viability of sperm from various local chickens during storage at 5℃ for 72 hours. Semen was collected every three days using the dorsal-abdominal massage from twelve chickens consisting of Kedu, Sentul and Pelung chicken. semen was diluted in extender contains 90% lactate ringer and 10% egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E (LREYSE). Sperm motility and viability was observed every 12 hours and the measurements were made up to 72 hours of storage. Complete random design repeated measurement with 4 replications was used in this study. One-way analysis of variance was used to analyze the data and followed by Duncan’s Multiple Range Test. The results showed sperm motility declined and sperm viability reduced during storage for all breeds. The significant declined of motility between breeds were only observed at 60 and 72 hours and the reduced viability between breeds was observed at 72 hours of storage. The declined sperm motility for Kedu chicken (31.59±3.26% and 75.36±1.25%) and Pelung chicken (36.11±4.05% and 75.83±5.34%) were significantly lower (p<0.05) than Sentul Chicken (50.39±2.60% and 95.00±5.00%) at 60 and 72 hours of storage respectively, while the decline sperm viability of Kedu chicken (57.59±3.64%) and Pelung chicken (54.39±5.73%) was significantly lower (p<0.05) than Sentul Chicken (90.30±9.70%) after 72 hours storage. It can be concluded that the reduction sperm motility and viability of Kedu and Pelung chicken is lower than Sentul chicken which are stored at 5℃ for 72 hours.
{"title":"Reduction Spermatozoa Motility and Viability on Various Local Chickens During Storage at 5℃","authors":"Nu'man - Hidayat, Chomsiatun Nurul Hidayah, A. P. Nugroho","doi":"10.20884/1.JAP.2020.22.3.64","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.64","url":null,"abstract":"The research compared the changes motility and viability of sperm from various local chickens during storage at 5℃ for 72 hours. Semen was collected every three days using the dorsal-abdominal massage from twelve chickens consisting of Kedu, Sentul and Pelung chicken. semen was diluted in extender contains 90% lactate ringer and 10% egg yolk extender with 0.025% sodium dodecyl sulfate and 2% vitamin E (LREYSE). Sperm motility and viability was observed every 12 hours and the measurements were made up to 72 hours of storage. Complete random design repeated measurement with 4 replications was used in this study. One-way analysis of variance was used to analyze the data and followed by Duncan’s Multiple Range Test. The results showed sperm motility declined and sperm viability reduced during storage for all breeds. The significant declined of motility between breeds were only observed at 60 and 72 hours and the reduced viability between breeds was observed at 72 hours of storage. The declined sperm motility for Kedu chicken (31.59±3.26% and 75.36±1.25%) and Pelung chicken (36.11±4.05% and 75.83±5.34%) were significantly lower (p<0.05) than Sentul Chicken (50.39±2.60% and 95.00±5.00%) at 60 and 72 hours of storage respectively, while the decline sperm viability of Kedu chicken (57.59±3.64%) and Pelung chicken (54.39±5.73%) was significantly lower (p<0.05) than Sentul Chicken (90.30±9.70%) after 72 hours storage. It can be concluded that the reduction sperm motility and viability of Kedu and Pelung chicken is lower than Sentul chicken which are stored at 5℃ for 72 hours.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"158-162"},"PeriodicalIF":0.0,"publicationDate":"2021-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46206218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-12DOI: 10.20884/1.JAP.2020.22.3.60
C. Ogbu, Nwabueze Ibeneme
The study evaluated the growth performance, haematology, serum biochemistry, intestinal microbial count, and antimicrobial resistance profile of Escherichia coli (E. coli) from broiler chicks fed diets supplemented with antibiotics (neomycin, and oxytetracycline), and probiotic (Saccharomyces cerevisiae). One hundred and twenty Abor acre broiler chicks randomly alloted to four treatment groups (30 birds/group; 10 birds/replicate) were used in the 49 days study. Group one (G 1, control) were fed basal diet while G 2, 3, and 4 received basal diet containing S. cerevisiae (0.80g/kg; 108cfu/g), neomycin (0.50g/kg) and oxytetracycline (0.30g/kg), respectively. Results showed significant treatment effects on body weight, feed intake, linear body values, some haematological indices, intestinal, caecal and combined caecal and intestinal bacteria counts, diameter of E. coli inhibition zone, and mortality. Body weight and feed intake were significantly higher in the supplemented groups. Intestinal bacterial count was highest in neomycin and control groups (5.29 ± 0.01 and 5.22 ± 0.02 Log10 cfu/ml, respectively) while S. cerevisiae and neomycin groups yielded the highest caecal, and combined caecal and intestinal bacterial counts. Eimeria Oocyst count did not differ significantly between groups. Escherichia coli from antibiotic fed groups had reduced sensitivity or were resistant to the antibiotics. It was concluded that subtherapeutic use of antibiotics as growth promoters in broiler chickens caused the development of antibiotic resistance, and therefore, should be avoided.
{"title":"Comparative Antibiotic and Probiotic Effects on Antimicrobial Sensitivity of Escherichia coli Isolates and Performance of Broiler Chickens","authors":"C. Ogbu, Nwabueze Ibeneme","doi":"10.20884/1.JAP.2020.22.3.60","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.3.60","url":null,"abstract":"The study evaluated the growth performance, haematology, serum biochemistry, intestinal microbial count, and antimicrobial resistance profile of Escherichia coli (E. coli) from broiler chicks fed diets supplemented with antibiotics (neomycin, and oxytetracycline), and probiotic (Saccharomyces cerevisiae). One hundred and twenty Abor acre broiler chicks randomly alloted to four treatment groups (30 birds/group; 10 birds/replicate) were used in the 49 days study. Group one (G 1, control) were fed basal diet while G 2, 3, and 4 received basal diet containing S. cerevisiae (0.80g/kg; 108cfu/g), neomycin (0.50g/kg) and oxytetracycline (0.30g/kg), respectively. Results showed significant treatment effects on body weight, feed intake, linear body values, some haematological indices, intestinal, caecal and combined caecal and intestinal bacteria counts, diameter of E. coli inhibition zone, and mortality. Body weight and feed intake were significantly higher in the supplemented groups. Intestinal bacterial count was highest in neomycin and control groups (5.29 ± 0.01 and 5.22 ± 0.02 Log10 cfu/ml, respectively) while S. cerevisiae and neomycin groups yielded the highest caecal, and combined caecal and intestinal bacterial counts. Eimeria Oocyst count did not differ significantly between groups. Escherichia coli from antibiotic fed groups had reduced sensitivity or were resistant to the antibiotics. It was concluded that subtherapeutic use of antibiotics as growth promoters in broiler chickens caused the development of antibiotic resistance, and therefore, should be avoided.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"126-136"},"PeriodicalIF":0.0,"publicationDate":"2021-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47768538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-16DOI: 10.20884/1.JAP.2020.22.2.53
B. Suwignyo, F. X. D. Kurniawan, N. Suseno, R. Utomo, B. Suhartanto
The study was conducted to identify the effect of lighting duration and dolomite addition on the vegetative growth of alfalfa plants in second regrowth phase. The experiment was conducted in the greenhouse of the Laboratory of Forage and Pasture Science, Faculty of Animal Science, Universitas Gadjah Mada. It used a completely randomized design with 3x3-factorial using two factors and three replications. The first factor was lighting duration (C) with 3 levels (C0 = 12 hours, C1 = 14 hours, and C2 = 16 hours). The second factor was dolomite addition (D) with 3 levels (D0 = 0 ton/ha, D1 = 6 ton /ha, and D2 = 12 ton/ha). The results showed a significant effect so Duncan Multiple Range Test (DMRT) followed at 5% and 1% levels. The observed variables were plant height, the number of plant branches, the number of plant leaves, fresh forage production, and the chemical content of the plants in the form of dry matter (DM) and organic matter (OM). The addition of dolomite significantly affected the number of the plant branches, the number of plant leaves and the dry matter of the plants. A significant correlation was found between the two treatments and the number of leaves (C2D2) and a correlation was between the treatment and dry matter (C1D2).
{"title":"Productivity and Nutrient Content of the Second Regrowth Alfalfa (Medicago Sativa L.) with Different Photoperiod and Dolomite","authors":"B. Suwignyo, F. X. D. Kurniawan, N. Suseno, R. Utomo, B. Suhartanto","doi":"10.20884/1.JAP.2020.22.2.53","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.2.53","url":null,"abstract":"The study was conducted to identify the effect of lighting duration and dolomite addition on the vegetative growth of alfalfa plants in second regrowth phase. The experiment was conducted in the greenhouse of the Laboratory of Forage and Pasture Science, Faculty of Animal Science, Universitas Gadjah Mada. It used a completely randomized design with 3x3-factorial using two factors and three replications. The first factor was lighting duration (C) with 3 levels (C0 = 12 hours, C1 = 14 hours, and C2 = 16 hours). The second factor was dolomite addition (D) with 3 levels (D0 = 0 ton/ha, D1 = 6 ton /ha, and D2 = 12 ton/ha). The results showed a significant effect so Duncan Multiple Range Test (DMRT) followed at 5% and 1% levels. The observed variables were plant height, the number of plant branches, the number of plant leaves, fresh forage production, and the chemical content of the plants in the form of dry matter (DM) and organic matter (OM). The addition of dolomite significantly affected the number of the plant branches, the number of plant leaves and the dry matter of the plants. A significant correlation was found between the two treatments and the number of leaves (C2D2) and a correlation was between the treatment and dry matter (C1D2).","PeriodicalId":7887,"journal":{"name":"Animal production","volume":"22 1","pages":"74-81"},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42957275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-16DOI: 10.20884/1.JAP.2020.22.2.51
I. Ismoyowati, I. Suswoyo, R. Rosidi, S. Mugiyono, Nu'man - Hidayat
Duck is one of the commodities that contribute to the national livestock production and Central Java is home to the fourth biggest duck population after West Java, South Sulawesi and East Java. The 2019 egg production in Central Java was 36.174 tons or 11.3% of the total egg production nationwide. Accordingly, it is important to investigate the effect of environmental factor, total number and age of ducks on egg production in Central Java. Specifically, this study aimed to observe the effect of region on egg production and feed conversion ratio (FCR) and the total livestock (chicken), age (month), stocking density (ducks/m2), internal housing temperature (oC), and humidity (%) on Hen day production/HDP. A survey engaging purposive random sampling was conducted on the population of duck farming in Central Java especially Tegal, Pemalang and Brebes districts. The data were subjected to General Linear Model (GLM) and a regression-correlation analysis using an SPSS program. The result showed that region significantly affected hen day production (HDP) but did not affect FCR. Housing temperature affected HDP by 14.9% and the higher the temperature, the lower the HDP. Duck age affected HDP by 11.7%, and the older the ducks the lower the egg production. Duck population, stocking density and humidity did not significantly affect egg production. Conclusively, duck egg production (HDP) is significantly affected by temperature inside the housing and the duck age.
{"title":"The Effect of Environmental Factor, Population and Age of Duck on Egg Production","authors":"I. Ismoyowati, I. Suswoyo, R. Rosidi, S. Mugiyono, Nu'man - Hidayat","doi":"10.20884/1.JAP.2020.22.2.51","DOIUrl":"https://doi.org/10.20884/1.JAP.2020.22.2.51","url":null,"abstract":"Duck is one of the commodities that contribute to the national livestock production and Central Java is home to the fourth biggest duck population after West Java, South Sulawesi and East Java. The 2019 egg production in Central Java was 36.174 tons or 11.3% of the total egg production nationwide. Accordingly, it is important to investigate the effect of environmental factor, total number and age of ducks on egg production in Central Java. Specifically, this study aimed to observe the effect of region on egg production and feed conversion ratio (FCR) and the total livestock (chicken), age (month), stocking density (ducks/m2), internal housing temperature (oC), and humidity (%) on Hen day production/HDP. A survey engaging purposive random sampling was conducted on the population of duck farming in Central Java especially Tegal, Pemalang and Brebes districts. The data were subjected to General Linear Model (GLM) and a regression-correlation analysis using an SPSS program. The result showed that region significantly affected hen day production (HDP) but did not affect FCR. Housing temperature affected HDP by 14.9% and the higher the temperature, the lower the HDP. Duck age affected HDP by 11.7%, and the older the ducks the lower the egg production. Duck population, stocking density and humidity did not significantly affect egg production. Conclusively, duck egg production (HDP) is significantly affected by temperature inside the housing and the duck age.","PeriodicalId":7887,"journal":{"name":"Animal production","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44543752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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