Animals最新文献

Ornithobacterium rhinotrachealis (ORT) and Pasteurella multocida (PM) are two major bacterial pathogens affecting the United States (US) commercial turkey industry. This retrospective observational case-case study aimed to investigate the association between land cover and confirmed disease occurrences attributed to PM or ORT in commercial turkey sites located in the Midwestern US A total of 65 farms from one poultry production company were included, where 28 had PM disease occurrences and 37 had ORT disease occurrences between 2014 and 2021. Risk factors of interest included land cover types (wetlands, forest, urban, pasture, herbaceous, barren, shrub), poultry-farm density in the area, and season and year of confirmed outbreak(s). A multivariable logistic regression model revealed that for every 1 m increase in distance from a farm to the nearest wetland, the odds of a confirmed disease occurrence related to PM decreased by approximately 0.24% compared to an ORT-related disease occurrence (p = 0.004). Meanwhile, PM occurrence during 2014-2017 was 98.5% higher than 2018-2019 and 93.2% higher than in 2020-2021. Broadly, the findings contribute to the dearth of research on land cover and turkey respiratory diseases and demonstrate that land cover is an important consideration for farm management and future study.

As an important class of non-coding RNAs, miRNAs participate in a variety of biological processes, including cell proliferation, differentiation, apoptosis, and hormone secretion. However, little is known about the role of pituitary miRNAs in follicular development in FecB mutant (Fecundity Booroola) sheep. Given the critical role of the pituitary gland in follicular development, this study employed miRNA-seq technology to analyze pituitary transcriptome expression patterns during the follicular phase (F) and the luteal phase (L) of FecB mutant homozygous (BB) and wild-type (WW) of Small Tail Han sheep. This study identified differentially expressed miRNAs (DEMs) related to re-production. Of these, 10, 4, 10, and 4 were differentially expressed (DE) between BB_F and BB_L, WW_F and WW_L, BB_F and WW_F, and BB_L and WW_L, respectively. Bioinformatics analysis revealed the enrichment of the target genes of these DEMs in multiple GO terms linked to animal re-productive processes and KEGG signaling pathways. The follicular phase and luteal phase show significant enrichment in multiple pathways closely related to cell communication and signal transduction, including the MAPK signaling pathway, neuroactive ligand-receptor interaction, and the cAMP signaling pathway. In comparisons of genotypes, the calcium signaling pathway, the cAMP signaling pathway, and the MAPK signaling pathway are enriched. The miRNA-mRNA co-expression network indicates that novel121 and oar-miR-10b may regulate CUL4B and ZFAND5, respectively, playing crucial roles in sheep reproduction. Dual luciferase reporter gene assays hinted at a potential targeting relationship between novel-121 and DNMT3A. These findings elucidate the impact of pituitary miRNAs on follicular development influenced by FecB gene mutation, providing valuable insights into sheep breeding.

High concentrations of free fatty acids (FFAs) caused by negative energy balance render the cow more prone to inflammatory diseases in part due to an imbalance in the types of immune cells and their specific functions. We previously demonstrated that ORAI calcium release-activated calcium modulator 1 (ORAI1) was associated with increased CD4⁺ Th17 content, but the precise mechanisms remain unclear. The purpose of this study was to evaluate the efficacy of FFAs on CD4⁺ T cell inflammatory response. High FFAs in dairy cows caused the transcript level of the pro-inflammatory factor IL-17A, plasma concentration of IL-17A, and amount of intracellular IL-17A to increase while the transcript levels and intracellular amount of the anti-inflammatory factor FOXP3 were downregulated. These changes indicated Th17/Treg imbalance and inflammation in dairy cows with high FFA. Moreover, ORAI1 and SDS abundance was elevated in dairy cows with high FFAs by transcriptomics, QPCR, and Western blot. Knockdown of SDS (siSDS) did not alter ORAI1 expression in CD4⁺ T cells from high-FFA cows, while it decreased the expression of inflammatory factors. Transfection of CD4⁺ T cells using siRNA knockdown for ORAI1 (siORAI1) revealed that SDS and inflammatory factor abundance decreased. Serine can be catabolized to pyruvate by the action of serine dehydratase (SDS). Data from this study suggested that high FFAs caused by negative energy balance after calving regulates the Th17/Treg balance via SDS, but SDS does not regulate ORAI1 abundance. The above data suggested a pro-inflammatory mechanism in CD4⁺ T cells regulated by the ORAI1-sensitive SDS pathway in early postpartum cows experiencing high-FFA conditions. Thus, targeting this pathway may represent a new therapeutic and interventional approach for preventing immune-related disorders around parturition.

Cancer is a leading cause of death among companion animals, with many cases diagnosed at advanced stages when clinical signs have appeared, and prognosis is poor. Emerging diagnostic technologies, including Artificial Intelligence (AI)-enhanced imaging, liquid biopsies, molecular diagnostics, and nematode-based screening, can improve early detection capabilities in veterinary medicine. These tools offer non-invasive or minimally invasive methods to facilitate earlier detection and treatment planning, addressing the limitations of traditional diagnostics, such as radiography and tissue biopsies. Recent advancements in comparative oncology, which leverage the biological similarities between human and companion animal cancers, underscore their translational value in improving outcomes across species. Technological advances in genomics, bioinformatics, and machine learning are driving a shift toward precision medicine, enabling earlier detection, personalized treatments, and monitoring of disease progression. Liquid biopsy testing detects circulating tumor DNA and tumor cells, providing actionable insights into tumor genetics without invasive procedures. Imaging systems enhance diagnostic precision, offering consistent and accurate tumor identification across veterinary practices, while portable innovations like Caenorhabditis elegans-based screening provide accessible options for underserved regions. As these technologies migrate from human medicine to veterinary applications, they are poised to redefine cancer care for companion animals. This review highlights key advancements in diagnostic technologies and their application in veterinary oncology, with a focus on enhancing early detection, accessibility, and precision in cancer care. By fostering the adoption of these innovations, veterinary oncology can achieve a new standard of care, improving outcomes for both animals and humans through the lens of comparative oncology.

Foot and Mouth disease (FMD) transmission is strongly related to the distribution and density of FMD-susceptible animals. In this study, we applied a model to simulate the spread of FMD in three Italian regions with different livestock demographics (densely, medium, and sparsely populated) in order to assess the cost effectiveness of the disease control options within the framework of the Italian contingency plan. The results of the simulations showed that stamping-out was sufficient to control the outbreak in the sparsely and medium populated areas whereas, in densely populated areas, only vaccinations could effectively control an outbreak. We also estimated the economic resources needed for the adoption of different control strategies, considering livestock indemnity, cleaning, disinfection and disposal, surveillance, vaccination and personnel costs. The results obtained showed that indemnity due to culling had the highest impact on the costs in all scenarios and vaccination resulted in the most cost-effective option to apply in densely populated livestock areas. The results of this study can be a useful aid to Competent Authorities in the preparation of contingency plans against FMD.

This study investigates the genetic architecture of the Kazakh Whiteheaded (KWH) cattle, applying population genetics approaches to resequenced genomes. F_ST analysis of 66 cattle breeds identified breeds for admixture analysis. At K = 19, the composite KWH breed showed contributions from Hereford, Altai, and Kalmyk cattle. Principal component analysis and ancestry inference confirmed these patterns, with KWH genomes comprising 45% Hereford, 30% Altai, and 25% Kalmyk ancestries. Haplotype analysis revealed 73 regions under putative selection in KWH, some shared with Hereford (e.g., with the gene DCUN1D4) and some KWH-specific (e.g., with the gene SCMH1). F_ST analysis identified 105 putative intervals under selection, with key genes (KITLG, SLC9C1, and SCMH1) involved in coat colour and physiological adaptations. Functional enrichment using The Database for Annotation, Visualization, and Integrated Discovery (DAVID) in selected regions highlighted clusters associated with developmental processes, ubiquitination, and fatty acid metabolism. Point F_ST identified 42 missense variants in genes enriched in functions related to economically important traits. Local ancestry inference revealed genomic intervals with predominantly non-Hereford ancestry, including high Altai (e.g., SCAPER) and Kalmyk (e.g., SRD5A2) contributions, while Hereford-dominated regions included genes ENO1 and RERE. This work elucidates the genomic contributions and adaptive signatures of selection shaping the KWH breed, providing candidate genetic variants for breeding program improvement and enhanced genome predictions.

The routine culling of male chicks in the laying hen industry raises significant ethical, animal welfare, and sustainability concerns. Current methods to determine chick embryo sex before hatching are costly, time-consuming, and invasive. This study aimed to develop a low-cost, non-invasive solution to predict chick embryo sex before hatching using the morphological features of eggs. A custom imaging apparatus was created using a smartphone and light box, enabling consistent image capture of chicken eggs. Egg length, width, area, eccentricity, and extent were measured, and machine learning models were trained to predict chick embryo sex. The wide neural network model achieved the highest accuracy of 88.9% with a mean accuracy of 81.5%. Comparison of the imaging apparatus to a high-cost industrial 3D scanner demonstrated comparable accuracy in capturing egg morphology. The findings suggest that this method can contribute to the prevention of up to 6.2 billion male chicks from being culled annually by destroying male embryos before they develop the capacity to feel pain. This approach offers a feasible, ethical, and scalable alternative to current practices, with potential for further improvements in accuracy and adaptability to different industry settings.

Condensed tannins (CTs) in certain leguminous forages can mitigate toxic alkaloid absorption linked to fescue toxicosis due to their high affinity towards various steroidal and protein-like alkaloids. However, their use as feed supplements remains underexplored. This study evaluated the impact of CT-rich sericea lespedeza (Lespedeza cuneata) pellets on the post-ingestive effects of fescue toxicosis. Twelve steers on wild-type endophyte-infected tall fescue pastures received either sericea lespedeza pellets (LES) or LES with polyethylene glycol (LPEG; negative control) for 12 weeks over three consecutive summers. Body weight, hair coat scores, temperatures (rectal and extremity), cortisol levels, and caudal artery lumen area were measured every four weeks. Steers fed LES showed trends toward higher ADG (p = 0.0999) and reduced hair retention (p = 0.0547) compared to those fed LPEG. Steers on LES also showed hotter tail skin temperatures (p = 0.0053) and cooler rectal temperatures (p < 0.0001) compared to those fed LPEG. LES-fed steers had a 21% larger caudal artery lumen area (p < 0.01), suggesting reduced vasoconstriction. Additionally, LES-fed steers tended to have lower hair cortisol (p = 0.0746), indicating reduced chronic stress. These results suggest that supplementation with CTs may alleviate the post-ingestive effects of fescue toxicosis, potentially by improving blood flow and reducing stress. However, further research is needed to determine whether CTs directly reduce alkaloid absorption, as well as to validate the long-term efficacy of CT supplementation.

This study strove to investigate the effect of boar age on conventional and non-conventional quality traits of fresh and liquid-stored semen. Sixty boars were allocated into 3 groups: 8-12 months (young); 24-36 months (adult); and 48-60 months (senior). Ejaculates were divided into two parts; the first one was assessed in native state while the second one was extended in the Androstar Plus extender containing gentamycin, stored at 5 °C and evaluated following 72 h. Young animals presented with a significantly lower sperm motility (p < 0.01), membrane and acrosome integrity (p < 0.0001), and mitochondrial activity (p < 0.0001) against adult boars. Significantly higher levels of free radicals and tumor necrosis factor alpha (p < 0.001), interleukin 1 and 6 (p < 0.0001) were found in young boars in comparison to adult boars. The assessment of liquid-stored semen revealed a significantly lower sperm motility, membrane, and acrosome integrity (p < 0.0001) in young boars when compared to adult boars. Moreover, Clostridium difficile, Escherichia coli, Pseudomonas aeruginosa, and Rothia nasimurium remained in liquid-stored semen obtained from young boars, while Corynebacterium sp. and Escherichia coli continued to be identified in samples collected from adult boars. In conclusion, age contributes to the overall quality of fresh as well as liquid-stored boar semen.