Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology最新文献

Objective: To study the relationship at molecular level between the resection range of laryngeal squamous cell carcinoma (LSCC) and prognosis.

Method: Immunohistochemistry method was used to detected the expression of EGFR, p27,Bcl-2 and p53 in surgically resected margins of the 23 patients with LSCC. The relationship between the genes and tumour recurrence were observed by the follow-up.

Result: Seven margin tissues of tumors had detectable Bcl-2 expression; eleven margin tissues overexpressed p27; and three expressed p53 and EGFR. Four of the 23 patients have had tumor recurrence and their expression of p27, p53 and EGFR were negative; the expression of Bcl-2 was positive in two patients of the four recurrences patients. The rest were alive unrelated causes without clinical (good outcome). Expression of neither EGFR nor p53 was associated with recurrence. Only p27 was significantly associated with outcome and disease-free survival.

Conclusion: These data indicate that assessing expression of multiple genes with immunohistochemistry in margin tissues is likely to be useful for surgically treated advanced laryngeal carcinoma. The expression of the p27 is more important.

Objective: To study the etiology, clinical manifestation, radiograph manifestation and treatment of osteogenic sarcoma in frontal sinus.

Method: A case of osteogenic sarcoma of the frontal sinus is reported. Recurrent epistaxis and supra-orbital pain were presented as the chief complaint. CT scan revealed a mass in right frontal sinus and invasion to the inferior wall of the sinus. Treatment comprised lateral rhinotomy followed by radiotherapy and chemotherapy.

Result: The patient was followed up 1 year and 9 month, and no recurrence or metastasis was found.

Conclusion: The diagnosis of osteogenic sarcoma in frontal sinus should be made upon the following aspects: CT scan of the sinus, nasal endoscopic detection and biopsy. Radical surgery followed by radiotherapy and chemotherapy may be the main treatment.

Objective: To observe effects of keratinocyte growth factor (KGF) on the proliferation of Hep-2 cell line; the activity and expression of extracellular signal-regulated kinase (ERK).

Method: Cell line proliferation was measured by Flow cytometry and MTT assay; ERK activity was measured by ERKl/2 immuno-precipitation kinase assay kit; and p ERK expression was assessed by Western blot.

Result: KGF can improve the proliferation of Hep-2 cell line. The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF.

Conclusion: KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.

Objective: To discuss the clinical application of Ephedrine Test in acoustic rhinometry, we studied the method of quantitative evaluation for nasal mucosal state and discriminated the objective parameters between simple rhinitis and hypertrophic rhinitis.

Method: Nasal cavity of volunteers from normal, simple and hypertrophic rhinitis patients were tested by acoustic rhinometry, the Nasal Airway Resistance (NAR), Nasal Cavity Volume (NCV), Nasal Minimal Cross-section Area (NMCA) and Distance of the minimal Cross-sectional Area from the Nostril (DCAN), their difference value and variational rate of pre or post Ephedrine Test were analyzed and compared.

Result: The paired-samples data of pre-post Nasal Ephedrine Test showed that NAR, NCV, NMCA and DCAN had extremely significant difference (P < 0.01). NCV and NMCA had increased, NAR had reduced and DCAN had "shifted forward" after Ephedrine Test. All the parameters had extremely difference (P < 0.01) among multi-group at pre or post Ephedrine Test. Only NCV had significant difference before Ephedrine Test (P < 0.05). However, after Ephedrine Test, all of the parameters (include difference value and variational rate) had significant difference (P < 0.05 or P < 0.01) between simple and hypertrophic rhinitis patients. Compared with volunteers of normal nasal cavity: all of the parameters (include difference value and variational rate) had significant difference (P < 0.05 or P < 0.01) in simple rhinitis patients, but some of the parameters of their difference and variational rate had not significant in hypertrophic rhinitis patients.

Conclusion: The acoustic rhinometry test can objectively estimate the state of nasal mucosal and get more information combine with Ephedrine Test. It can expand the clinical application of acoustic rhinometry and is worth while recommending.

Objective: To investigate the nature of juvenile nasopharyngeal angiofibroma (JNA).

Method: The difference of PCNA, VEGF and bFGF expression between hemangioma and vascular malformation in children and infant was applied to this study. 25 cases of JNA,10 cases of hemangioma and 11 cases of vascular malformation were studied respectively by immunohistochemical methods.

Result: The PCNA and VEGF expression in the endothelial cells of JNA were negative, the PCNA and VEGF expression in the endothelial cells of haemangioma were positive, the PCNA and VEGF expression in the endothelial cells of vascular malformation were negative, there were extraordinary significant difference between the group of JNA and haemangioma (P < 0.01). There were no significant difference between the group of JNA and vascular malformation ( P > 0.05). The bFGF expression in the endothelial cells of JNA was positive, the bFGF expression in the endothelial cells of haemangioma was positive, the bFGF expression in the endothelial cells of vascular malformation was negative, there were no significant difference between the group of JNA and haemangioma (P > 0.05). There were extraordinary significant difference between the group of JNA and vascular malformation (P < 0.01).

Conclusion: The nature of JNA could be a congenital proliferative vascular malformation.

Objective: To study the expression of tumor necrosis factor related apoptosis induce ligand (TRAIL) receptors (DR4, DR5, DcR1, DcR2) in human laryngeal squamous cell carcinoma (LSCC).

Method: The expression and distribution of DR4, DR5, DcR1, DcR2 were detected by immunohistochemical method in 68 patients of LSCC and 40 laryngeal normal tissues (LNT).

Result: All of TRAIL receptors was observed in both tissues of LSCC and LNT. It was found that DR4, DR5 were overexpressed in the two tissues, indicating that there were no significant difference between the expressions of DR4 and DR5 in two tissues (P >0.05). DcR1 , DcR2 were over-expressed in LNT,were low expressed in LSCC (P <0. 05). Level of the TRAIL receptors DR5, DcR2 related with the degree of LSCC histological differentiation (P <0. 05). All receptors were not related with different clinical stage (P >0.05).

Conclusion: TRAIL receptors expression is generally expressed in human LSCC and LNT. The expression level of TRAIL receptors is various, which may explain the anti-cancer effect of TRAIL. The TRAIL receptor DcR1, DcR2 may play an important role in the apoptosis regulation of LSCC.

Objective: To study the expression of eukaryotic initiation factor4E (eIF4E)and matrix metalloproteinase-9 (MMP-9) in supraglottic, hypopharyngeal carcinoma, different distance surgical margins and lymphatic metastasis; to study the relationship of two molecular markers.

Method: Expression of elF4E and MMP-9 in carcinoma and different margins were detected by HE-stained, routine pathological section, immunohistochemical SP method and image analysis.

Result: (1) Immunohistochemical staining for MMP-9 was performed with a monoclonal anti-MMP-9 antibody. The positive expression rate was 68. 4 % . There was significant difference between lymph node metastasis and no lymph node metastasis, but there was no significant difference between T3 and T4 or T1 and T2 ( P >0. 05). There was no significant difference between MMP-9 and carcinoma position,pathology grade ( P> 0. 05). Positive expression rate of MMP-9 was decreased in tumour , 5 mm, 10 mm different distant margin of supraglottic and hypopharyngeal carcinoma as tissue progressed adjacent to carcinoma. (2) Staining positive rate of elF4E in supraglottic and hypopharyngeal carcinoma was 89%. There was significant association between eIF4E expression intensity and clinical stage, lymphatic metastasis. The positive expression rate of eIF4E was increased in sequence from 10 mm, 5 mm distant mucosa tissue to carcinoma nest in supraglottic and hypopharyngeal carcinoma. (3) Negative expression of MMP-9 and eIF4E was seen in the normal lymph nodes. MMP-9 positive expression rate was 28% in metastasis lymph nodes,elF4E was 42 %. (4) There was no significant correlation between elF4E and MMP-9 in margins according to spearman rank correlation test.

Conclusion: eIF4E and MMP-9 expression was significantly higher in supraglottic and hypopharyngeal carcinoma than distant margin and normal mucosa tissue. elF4E positive rate was higher than MMP-9,expression sensitivity and reliability of elF4E in margins was higher than MMP-9. elF4E is a sensitive marker in growth, infiltration and metastasis of supraglottic and hypopharyngeal carcinoma.

Objective: To study the expression and clinical significance of Endothelin-1 in laryngeal carcinoma.

Method: Immunohistochemical (SP) method was used to detect the expression of Endothelin-1 in 30 cases of laryngeal carcinoma,20 cases of laryngeal benign lesions and 10 cases of. The relationship between the expression of Endothelin-1 and the clinic stage was analysed by SPSS12. 0 software. RESULT. The positive expression of Endothelin-1 in all three type tissues. There is a significant difference between the laryngeal carcinoma and the laryngeal benign lesions, the laryngeal carcinoma and the normal laryngeal mucous membrane, the laryngeal benign lesions and the normal laryngeal mucous membrane( P <0. 05). The highest expression of Endothelin-1 is in laryngeal carcinoma tissue. In laryngeal carcinoma tissue, the advanced clinical stage, the more higher expression of endothelin-1.

Conclusion: Positive expression of Endothelin-1 in laryngeal carcinoma tissue was highest in three tissue. It may be play an important role in carcinogenesis and progress of laryngeal carcinoma.

Objective: To investigate the relationship between the expression of matrix metalloproteinase-2 (MMP-2) and clinical significance of laryngeal squamous cell carcinomas (LSCC).

Method: Expression of the MMP-2 was detected by immunohistochemical method in 63 cases of LSCC, 20 cases of adjacent safety margin (ASM) and 18 cases of vocal cord polyp (VCP).

Result: The positive percentage of active MMP-2 expression were 66.7%, 33. 3% and 15. 0%, in LSCC,VCP and ASM respectively. Significant differences ( P <0. 05) were observed among the three group; there was a significant differences in the expression of MMP-2 among the different pathologic degree I , II , III; no significant difference in the expression of MMP-2 between lymphoid metastasis and no lymphoid metastasis ( P >0. 05); Age and clinical stage did not change the expressions of the MMP-2 expression ( P <0. 05).

Conclusion: MMP-2 probably plays an important role in the carcinogenesis and progression of LSCC. But it may be an unnecessary factor in the metastases of LSCC.

Objective: Matrix metalloproteinases (MMPs) are key factors which influence the grades and/or metastasis of stereo tumour. There are short of research of MMP-12 now. The experiment is done to investigate the expression of MMP-12 and NM_002426, and relationship between the two genes and the biologic behavior in squamous laryngeal carcinoma.

Method: Thirty pairs of specimens, include supraglottic carcinoma tissue and normal mucosa, were chosen. Three gene arrays, based on different kinds of malignant, were made to study NM_ 002426 at first, then immunohistochemical method were used to observe the difference of MMP-12 between tumour and normal mucosa, metastasis group and no-metastasis group.

Result: NM_002426 express high ratio in all 3 chips, and have the highest mark in both mid-level and high-level malignant tissue. The immunohistochemical results showed that MMP-12 had significant difference between tumour and normal mucosa, metastasis group and no-metastasis group. The MMP-12 expression was unrelated to different malignant degree.

Conclusion: NM_ 002426 has significant hyperplasia in squamous laryngeal carcinoma, which caused high expression of MMP-12,and it makes a main contribution to the carcinogenesis and metastasis of laryngeal carcinoma. MMP-12 and its gene overexpress may be a auxiliary diagnosis method.