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A CAI System for Simulation of Enzymes Kinetics 酶动力学模拟CAI系统
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00070-9
G. G. Amorim, F. S. Leão, M. R. Martins, Joana D. R. Santos, M. D. Novaes, J. L. L. Filho
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引用次数: 1
Bioconversion of waste paper materials to sugars: an application illustrating the environmental benefit of enzymes 废纸原料生物转化为糖:说明酶的环境效益的应用
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00053-9
J.P.H. van Wyk, M.A. Mogale, K.S. Moroka

Recycling of waste materials into useful products is very topical not only to protect natural resources, but also to limit environmental pollution. The enzymatically catalyzed bioconversion of organic based waste such as used paper materials to soluble sugars like glucose can be performed in an environmental-friendly way. A basic experiment is proposed to show how different paper materials exhibit varying susceptibilities towards cellulase catalyzed saccharification, and that this approach can be applied by biochemistry or biotechnology students in the bioconversion of different cellulose related waste materials.

将废物回收利用成有用的产品不仅可以保护自然资源，还可以限制环境污染。酶催化有机废物(如用过的纸张材料)转化为可溶性糖(如葡萄糖)的生物转化可以以一种环保的方式进行。提出了一个基本的实验，以显示不同的纸张材料对纤维素酶催化糖化的不同敏感性，并且这种方法可以由生物化学或生物技术学生应用于不同纤维素相关废物的生物转化。
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引用次数: 11
Product review 产品评论
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00112-0
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引用次数: 0
Sex determination using the polymerase chain reaction 用聚合酶链反应测定性别
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00044-8
Anthony S. Weiss, Jill M. Johnston

A practical class experiment on the PCR is described which has been used over several years as part of an undergraduate biochemistry and molecular biology course for science students. A major aim is to provide experience in the use of the polymerase chain reaction (PCR) and its interpretation. Students are given small coded DNA samples and use the PCR reaction to determine whether the sample is from a male or a female.

本文描述了一个关于聚合酶链反应的实践课堂实验，该实验作为本科生生物化学和分子生物学课程的一部分已经使用了几年。主要目的是提供使用聚合酶链反应(PCR)及其解释的经验。学生们得到了小的编码DNA样本，并使用PCR反应来确定样本是来自男性还是女性。
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引用次数: 2
Product review 产品评论
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00070-9
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引用次数: 1
Index 指数
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00118-1
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引用次数: 0
Product review 产品评论
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00063-1
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引用次数: 0
Second IUBMB workshop on biochemical education in Bucharest, Rumania, september 21–23, 1998 1998年9月21日至23日在罗马尼亚布加勒斯特举行的第二届生物化学教育讲习班
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00057-6
C. Sandu
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引用次数: 2
An experiment illustrating DNA-protein interactions using nuclear extracts from chicken erythrocytes 利用鸡红细胞核提取物说明dna -蛋白相互作用的实验
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00066-7
Beatriz Garat

The electrophoretic mobility shift assay is a simple and rapid method for visualizing the existence of specific DNA-protein interactions and is a useful teaching experiment. In the experiment described here the students prepare nuclear extracts, a DNA probe, and can successfully produce specific DNA-protein complexes and estimate physicochemical parameters characterizing the interaction. The system described is easy to perform. The synthesis of the oligonucleotides required and the labeled deoxyribonucleotide used for labeling the probe are the main costs of the experiment, but since the synthesized oligonucleotides can be used in several courses and the amount of labeled deoxyribonucleotide required for labeling the probe is small the experiment is relatively inexpensive to put on with a class.

电泳迁移率转移试验是一种简单、快速的观察dna -蛋白特异性相互作用存在的方法，是一种有用的教学实验。在这里描述的实验中，学生准备核提取物，DNA探针，并可以成功地产生特定的DNA-蛋白质复合物，并估计表征相互作用的物理化学参数。所描述的系统易于执行。合成所需的寡核苷酸和标记的脱氧核糖核苷酸用于标记探针是实验的主要成本，但由于合成的寡核苷酸可以用于几个课程，标记探针所需的标记的脱氧核糖核苷酸的数量很少，因此在一个班级进行实验相对便宜。
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引用次数: 2
A laboratory class experiment to detect and quantify lovastatin in commercial medicaments 商用药物中洛伐他汀的检测与定量实验
Biochemical education
Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00058-8
Walter Giordano, Carlos E. Domenech

This article describes a laboratory class experiment for an undergraduate Biochemistry course for Chemistry students. The experimental work involves the extraction of lovastatin from a medicament utilized in the treatment of hypercholesterolemic patients, and its determination by spectroscopy and by reversed-phase high-performance liquid chromatography. The entire experiment can be performed in one laboratory period, e.g. less than one day.

本文介绍了化学专业本科生物化学课程的实验课堂实验。实验工作包括从治疗高胆固醇血症患者的药物中提取洛伐他汀，并通过光谱和反相高效液相色谱法测定其含量。整个实验可以在一个实验周期内完成，例如不到一天。
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引用次数: 2
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