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Product review 产品评论
Biochemical education
Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00134-X
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引用次数: 0
Biotechnology education 生物技术教育
Biochemical education
Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00131-4
R.O. Jenkins (Editor)
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引用次数: 3
Standards for the Ph.D. Degree in the Molecular Biosciences 分子生物科学博士学位标准
Biochemical education
Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00123-5
F. Vella, Leopoldo de Meis, Alan H. Mehler, Wilfried Rombauts, Harold B. White III, E.J. Wood
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引用次数: 3
Calculating apparent equilibrium constants of enzyme-catalyzed reactions at pH 7. 计算pH值为7时酶催化反应的表观平衡常数。
Biochemical education
Pub Date : 2000-01-01
Alberty

Apparent equilibrium constants K' of biochemical reactions at pH 7 and standard apparent reduction potentials of half reactions at pH 7 can be calculated using a table of standard transformed Gibbs energies of formation Delta(f)G'(0) at pH 7. A table is provided for 136 reactants at 25 degrees C, pH 7, and ionic strengths of 0, 0.10, and 0.25 M. Examples are given to illustrate the use of the table.

pH为7时生化反应的表观平衡常数K′和pH为7时一半反应的标准表观还原势可以用pH为7时的标准转化吉布斯生成能(f)G′(0)表计算。给出了在25℃，pH为7，离子强度为0,0.10和0.25 m时136种反应物的表，并给出了示例来说明该表的使用。
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引用次数: 0
alpha-Amylase production by solid state fermentation: a new practical approach to biotechnology courses. 固体发酵法生产α -淀粉酶:生物技术课程的实用新方法。
Biochemical education
Pub Date : 2000-01-01 DOI: 10.1111/j.1539-3429.2000.tb00056.x
Mulimani, Patil Ramalingam GN
A laboratory practical experiment in Biotechnology involving the investigation of alpha-amylase production by solid state fermentation of Gibberella fujikuroi is described.
介绍了一项生物技术的实验室实践实验，涉及研究藤黑赤霉素固态发酵生产α -淀粉酶。
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引用次数: 26
Product review 产品评论
Biochemical education
Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00085-0
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引用次数: 0
Understanding ion transport by quantification of mitochondrial swelling. 通过量化线粒体肿胀了解离子转运。
Biochemical education
Pub Date : 2000-01-01
Königsberg

Suspensions of mitochondria are turbid and scatter light. An increase in the matrix volume (swelling) due to the influx of permeable solutes results in a decrease in the amount of light scattered. This property can be used to study solute fluxes across the mitochondrial inner membrane. A rapid method for isolating mitochondria is presented along with three swelling experiments using energized and non-energized mitochondria to illustrate ion transport across energy transducing membranes.

线粒体悬浮液浑浊且散射光线。由于渗透性溶质的涌入，基质体积(膨胀)的增加导致光散射量的减少。这一性质可用于研究线粒体内膜的溶质通量。提出了一种快速分离线粒体的方法，以及三个使用带电和非带电线粒体的膨胀实验，以说明离子在能量转导膜上的运输。
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引用次数: 0
beta-Galactosidase immobilization for milk lactose hydrolysis: a simple experimental and modelling study of batch and continuous reactors. 乳糖水解-半乳糖苷酶固定化:间歇式和连续式反应器的简单实验和模型研究。
Biochemical education
Pub Date : 2000-01-01 DOI: 10.1111/j.1539-3429.2000.tb00057.x
Obón, Castellar, Iborra, Manjón
The experiment described in this paper introduces students to the practical use of an enzyme (beta-galactosidase, or lactase) acting on a natural substrate. The enzyme is immobilized onto a cheap support, and the immobilized derivative is used in a packed-bed reactor for continuous milk lactose hydrolysis. The results are compared to those obtained for discontinuous batch reactors with soluble enzyme. A mathematical model of the two types of reactors is run, and its results are compared with the experimental data obtained.
本文中描述的实验向学生介绍了作用于天然底物的酶(β -半乳糖苷酶或乳糖酶)的实际应用。将酶固定在廉价的载体上，固定化衍生物在填充床反应器中用于连续乳糖水解。并与间歇式间歇式可溶酶反应器的实验结果进行了比较。建立了两种反应器的数学模型，并与实验结果进行了比较。
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引用次数: 7
Plant protoplast isolation - a practical approach. 植物原生质体分离——一种实用的方法。
Biochemical education
Pub Date : 2000-01-01
Prasad, Naik

A method for the isolation of plant protoplasts is presented that uses inexpensive sources of enzymes. It is suitable for student use in Biotechnology.

提出了一种利用廉价酶源分离植物原生质体的方法。它适合学生在生物技术方面使用。
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引用次数: 0
Chemical cross-linking analysis of Ca(2+-)ATPase from rabbit skeletal muscle. 兔骨骼肌Ca(2+-) atp酶的化学交联分析。
Biochemical education
Pub Date : 2000-01-01
Murray, Ohlendieck

A student laboratory experiment is described dealing with chemical cross-linking analysis of Ca(2+-)ATPase from rabbit muscle. The experiment introduces students to a wide range of techniques including subcellular fractionation, chemical cross-linking, polyacrylamide gel electrophoresis, blotting and immunochemical detection of proteins.

一个学生的实验室实验描述了处理化学交联分析Ca(2+-) atp酶从兔子肌肉。该实验向学生介绍了广泛的技术，包括亚细胞分离、化学交联、聚丙烯酰胺凝胶电泳、印迹和免疫化学检测蛋白质。
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引用次数: 0
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