Although fluorescence is a powerful and much used tool in Biology there are very few applications for teaching this topic in Biochemistry courses. Some classical experiments in carboxyfluorescein fluorescence are presented here, including recording a fluorescence spectrum, quenching by oxygen, effect of concentration and pH on fluorescence signal, and a Stern-Volmer representation for a dynamic quenching.
This article defines and describes some of the basics of bioinformatics and projects aimed at sequencing entire genomes. Emphasis is placed on some of the ways in which the primary structures of nucleic acids and proteins may be investigated and analysed to gain meaningful biological information using computers and appropriate software. The importance of the world wide net and access to it is given prominence, particularly in bioinformatics research and teaching.
The main objective of this laboratory practical class was to teach students how a detergent and the best experimental conditions are chosen to solubilize a given membrane protein. Kidney Na,K-ATPase was chosen as the protein of interest and anionic, neutral and zwitterionic detergents were tested. Simple laboratory experiments were designed to study the effect of the detergent on the activity of the enzyme, the effect of detergent concentration on solubilization, the effect of protein concentration on enzyme solubilization, and the effect of time and temperature of incubation during enzyme solubilization. This resulted in the selection of an appropriate detergent for the solubilization of the protein taking into account smaller inactivation factors, more effective solubilization (more effective solubilization with a better detergent-protein relationship), lower inactivation temperature and time of incubation of the membrane protein with the detergent. The results obtained showed that instantaneous incubation of Na,K-ATPase with C(12)E(8) (1:1 w/w) at 4 degrees C resulted in a more efficient solubilization and had a smaller denaturing effect on the solubilized enzyme.
The experiment described in this paper introduces students to the practical use of an enzyme (β-galactosidase, or lactase) acting on a natural substrate. The enzyme is immobilized onto a cheap support, and the immobilized derivative is used in a packed-bed reactor for continuous milk lactose hydrolysis. The results are compared to those obtained for discontinuous batch reactors with soluble enzyme. A mathematical model of the two types of reactors is run, and its results are compared with the experimental data obtained.
The first five years of a new program to organize high-level scientific research training for gifted high school students in Hungary are described. Besides giving unique research opportunities for talented students in their most receptive age, the program already helped the establishment of almost 100 scientific research clubs in Hungarian high schools, provided a focal point for science training of high school teachers and helped regional cooperation in Central-Eastern Europe.
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