Pub Date : 2000-05-01DOI: 10.1016/S0307-4412(00)00005-4
Norbert Latruffe, Shawn J. Hassell
Peroxisome proliferation in rodent liver is a good biochemical marker of toxicology for several classes of xenobiotics, including fibrates; phthalates and adipates; or chlorophenoxy-acetate, a herbicide. Research in peroxisomes provides a good example of the integration of fields related to basic sciences and biomedical and industrial health. A 25 min video programme illustrates techniques involved in the characterization of purified peroxisomes (Fig. 1
Download : Download high-res image (70KB)
Download : Download full-size image
Fig. 1. Electron microscope view of isolated peroxisomes (from rat liver ×50 000).
) and membranes, immunoblotting, measurement of proliferation markers, mRNA analysis at the post-transcriptional level, DNA techniques, cell cultures as biological models and computer analysis. It is aimed at undergraduates and non-biochemist advanced students in biology.
{"title":"Peroxisomes: biochemistry, molecular biology and genetic diseases— a video programme for teaching students","authors":"Norbert Latruffe, Shawn J. Hassell","doi":"10.1016/S0307-4412(00)00005-4","DOIUrl":"https://doi.org/10.1016/S0307-4412(00)00005-4","url":null,"abstract":"<div><p><span>Peroxisome proliferation in rodent liver is a good biochemical marker of toxicology for several classes of xenobiotics, including fibrates; phthalates and adipates; or chlorophenoxy-acetate, a herbicide. Research in peroxisomes provides a good example of the integration of fields related to basic sciences and biomedical and industrial health. A 25</span> <!-->min video programme illustrates techniques involved in the characterization of purified peroxisomes (<span>Fig. 1</span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (70KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span><span><span><p><span>Fig. 1</span>. <span>Electron microscope view of isolated peroxisomes (from rat liver ×50</span> <!-->000).</p></span></span></figure></span>) and membranes, immunoblotting, measurement of proliferation markers, mRNA analysis at the post-transcriptional level, DNA techniques, cell cultures as biological models and computer analysis. It is aimed at undergraduates and non-biochemist advanced students in biology.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 136-138"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00005-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91977645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-05-01DOI: 10.1016/S0307-4412(00)00027-3
J.M. Wallach
{"title":"Workshop on teaching of biochemistry in Europe","authors":"J.M. Wallach","doi":"10.1016/S0307-4412(00)00027-3","DOIUrl":"10.1016/S0307-4412(00)00027-3","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 139-140"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00027-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56596271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-05-01DOI: 10.1016/S0307-4412(99)00145-4
V.H. Mulimani, G.N. Patil, Ramalingam
A laboratory practical experiment in Biotechnology involving the investigation of α-amylase production by solid state fermentation of Gibberella fujikuroi is described.
介绍了一项研究藤黑赤霉素固态发酵生产α-淀粉酶的生物技术实验室实践实验。
{"title":"α-Amylase production by solid state fermentation: a new practical approach to biotechnology courses","authors":"V.H. Mulimani, G.N. Patil, Ramalingam","doi":"10.1016/S0307-4412(99)00145-4","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00145-4","url":null,"abstract":"<div><p>A laboratory practical experiment in Biotechnology involving the investigation of <em>α</em>-amylase production by solid state fermentation of <em>Gibberella fujikuroi</em> is described.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 161-163"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00145-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92143307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-05-01DOI: 10.1016/S0307-4412(99)00091-6
José Carlos Cameselle, Alicia Cabezas, José Canales, Marı́a Jesús Costas, Ángeles Faraldo, Ascensión Fernández, Rosa Marı́a Pinto, João Meireles Ribeiro
This article describes a `dry-laboratory’ practical in which the multi-step purification of an enzyme is simulated. It has been devised to be implemented with beginner undergraduates taking an introductory course in biochemistry, with the aim of giving them a glimpse of the intensity of effort involved in complex, research-oriented experiments. The purification steps simulated are: preparation of a liver soluble extract, ammonium sulfate fractionation, gel filtration, ion-exchange and dye-ligand affinity chromatography. Before the simulation, our students are familiar with the preparative and analytical techniques involved, through short `cookbook’ laboratory experiments and, in some cases, ad hoc demonstrations. For the simulation, the students are given detailed protocols of the preparative and analytical experiments, and the raw numeric or graphical data obtained. They have to perform the calculations and graphing necessary to produce a purification table. The 27-page Student Booklet needed to implement the practical is offered by the authors to interested teachers, as a printable electronic file.
{"title":"The simulated purification of an enzyme as a `dry’ practical within an introductory course of biochemistry","authors":"José Carlos Cameselle, Alicia Cabezas, José Canales, Marı́a Jesús Costas, Ángeles Faraldo, Ascensión Fernández, Rosa Marı́a Pinto, João Meireles Ribeiro","doi":"10.1016/S0307-4412(99)00091-6","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00091-6","url":null,"abstract":"<div><p>This article describes a `dry-laboratory’ practical in which the multi-step purification of an enzyme is simulated. It has been devised to be implemented with beginner undergraduates taking an introductory course in biochemistry, with the aim of giving them a glimpse of the intensity of effort involved in complex, research-oriented experiments. The purification steps simulated are: preparation of a liver soluble extract, ammonium sulfate fractionation, gel filtration, ion-exchange and dye-ligand affinity chromatography. Before the simulation, our students are familiar with the preparative and analytical techniques involved, through short `cookbook’ laboratory experiments and, in some cases, ad hoc demonstrations. For the simulation, the students are given detailed protocols of the preparative and analytical experiments, and the raw numeric or graphical data obtained. They have to perform the calculations and graphing necessary to produce a purification table. The 27-page Student Booklet needed to implement the practical is offered by the authors to interested teachers, as a printable electronic file.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 148-153"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00091-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136521743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2000-05-01DOI: 10.1016/S0307-4412(00)00029-7
Chris G. Whiteley
A graphical method for analysing enzyme data to obtain kinetic parameters, to identify the types of inhibition and the enzyme mechanisms is described. The method consists of plotting experimental data as v/(V0−v) versus 1/(I) at different substrate concentrations. I is the inhibitor concentration; V0 and v are the initial rates of enzyme reaction attained by the system in the presence of a fixed amount of substrate and in the absence and presence of inhibitor respectively. Complete inhibition gives straight lines that pass through the origin while partial inhibition gives straight lines that converge on the 1/I-axis at a point away from the origin. With uncompetitive inhibition the slopes of the lines decrease with increasing substrate concentration. The kinetic parameters Km, K′i and β (degree of partiality) can best be determined from respective secondary plots of slope and intercept versus reciprocal of substrate concentration.
{"title":"Enzyme kinetics: partial and complete uncompetitive inhibition","authors":"Chris G. Whiteley","doi":"10.1016/S0307-4412(00)00029-7","DOIUrl":"10.1016/S0307-4412(00)00029-7","url":null,"abstract":"<div><p>A graphical method for analysing enzyme data to obtain kinetic parameters, to identify the types of inhibition and the enzyme mechanisms is described. The method consists of plotting experimental data as <em>v</em>/(<em>V</em><sub>0</sub>−<em>v</em>) versus 1/(<em>I</em>) at different substrate concentrations. I is the inhibitor concentration; <em>V</em><sub>0</sub> and v are the initial rates of enzyme reaction attained by the system in the presence of a fixed amount of substrate and in the absence and presence of inhibitor respectively. Complete inhibition gives straight lines that pass through the origin while partial inhibition gives straight lines that converge on the 1/<em>I</em><span>-axis at a point away from the origin. With uncompetitive inhibition the slopes of the lines decrease with increasing substrate concentration. The kinetic parameters </span><em>K</em><sub>m</sub>, <em>K</em>′<sub>i</sub> and <em>β</em> (degree of partiality) can best be determined from respective secondary plots of slope and intercept versus reciprocal of substrate concentration.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"Pages 144-147"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(00)00029-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cameselle, Cabezas, Canales, Jesús Costas M, Faraldo, Fernández, María Pinto R, Meireles Ribeiro J
This article describes a 'dry-laboratory' practical in which the multi-step purification of an enzyme is simulated. It has been devised to be implemented with beginner undergraduates taking an introductory course in biochemistry, with the aim of giving them a glimpse of the intensity of effort involved in complex, research-oriented experiments. The purification steps simulated are: preparation of a liver soluble extract, ammonium sulfate fractionation, gel filtration, ion-exchange and dye-ligand affinity chromatography. Before the simulation, our students are familiar with the preparative and analytical techniques involved, through short 'cookbook' laboratory experiments and, in some cases, ad hoc demonstrations. For the simulation, the students are given detailed protocols of the preparative and analytical experiments, and the raw numeric or graphical data obtained. They have to perform the calculations and graphing necessary to produce a purification table. The 27-page Student Booklet needed to implement the practical is offered by the authors to interested teachers, as a printable electronic file.
{"title":"The simulated purification of an enzyme as a 'dry' practical within an introductory course of biochemistry.","authors":"Cameselle, Cabezas, Canales, Jesús Costas M, Faraldo, Fernández, María Pinto R, Meireles Ribeiro J","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This article describes a 'dry-laboratory' practical in which the multi-step purification of an enzyme is simulated. It has been devised to be implemented with beginner undergraduates taking an introductory course in biochemistry, with the aim of giving them a glimpse of the intensity of effort involved in complex, research-oriented experiments. The purification steps simulated are: preparation of a liver soluble extract, ammonium sulfate fractionation, gel filtration, ion-exchange and dye-ligand affinity chromatography. Before the simulation, our students are familiar with the preparative and analytical techniques involved, through short 'cookbook' laboratory experiments and, in some cases, ad hoc demonstrations. For the simulation, the students are given detailed protocols of the preparative and analytical experiments, and the raw numeric or graphical data obtained. They have to perform the calculations and graphing necessary to produce a purification table. The 27-page Student Booklet needed to implement the practical is offered by the authors to interested teachers, as a printable electronic file.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 3","pages":"148-153"},"PeriodicalIF":0.0,"publicationDate":"2000-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21721012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号