Anaerobe最新文献_第2页

Effect of pretreatment with microbial consortium on anaerobic digestion of rice drying residue 微生物联合体预处理对水稻干燥渣厌氧消化的影响。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-22 DOI: 10.1016/j.anaerobe.2025.103020

Ana María Espinosa Negrín , Lisbet Mailín López González , Alexander Calero Hurtado , Deborah Henderson , Janet Jiménez Hernández

Objective

Residues from rice drying are an important source of lignocellulosic biomass, with the potential to be used in anaerobic digestion processes and contribute to fossil fuels substitution. However, due to its recalcitrant structure, bioconversion remains a challenge. This research aimed to assess the effect of pretreatment with a microbial consortium on hydrolysis, methane yield, and anaerobic digestion kinetics of this residue.

Methods

For the study, a new consortium (MC) was obtained and characterized. This consortium was then used (diluted 1:100) to pretreat the substrate for five days, using two ratios (12 and 18 mL of MC g volatile solids (VS)⁻¹ of substrate). The degradation capacity was evaluated through the reducing sugar content released daily, in order to select the optimal time and ratio. The kinetic behavior in anaerobic digestion was described by four models: Roediger, Hill, Modified Gompertz and Transference Function.

Results

Characterization revealed the presence of bacteria with cellulolytic activity in the consortium. The highest methane yield was obtained for pretreated residues (190.8 L_N kgVS⁻¹) after 24 h of pretreatment at the ratio of 12 mL gVS⁻¹, which was 13.9 % higher than the untreated substrate. All models fit well with the experimental cumulative methane yield data, but Roediger model showed the best fit in all cases.

Conclusion

This research demonstrated that pretreatment with a microbial consortium significantly enhanced hydrolysis, methane yield, and anaerobic digestion kinetics of the rice drying residue. The findings highlight its potential to optimize biogas production, offering a sustainable strategy for improving waste-to-energy conversion efficiency.

目的：大米干燥残留物是木质纤维素生物质的重要来源，具有用于厌氧消化过程和有助于化石燃料替代的潜力。然而，由于其顽固性结构，生物转化仍然是一个挑战。本研究旨在评估微生物联合体预处理对该残渣水解、甲烷产率和厌氧消化动力学的影响。方法：制备了一种新的共聚物（MC），并对其进行了表征。然后使用该联合体（稀释1:100）预处理底物5天，使用两种比例（12和18 mL MC g挥发性固体(VS)-1的底物）。通过日还原糖释放量评价其降解能力，选择最佳时间和配比。厌氧消化的动力学行为用Roediger、Hill、修正Gompertz和传递函数四种模型来描述。结果：鉴定显示该菌群中存在具有纤维素分解活性的细菌。以12 mL gVS-1的比例预处理24 h后，预处理残渣的甲烷产率最高（190.8 LN kgVS-1），比未处理底物提高13.9%。所有模型都与实验累积甲烷产量数据拟合良好，但Roediger模型在所有情况下都表现出最佳拟合。结论：该研究表明，微生物群落预处理显著提高了大米干燥残渣的水解、甲烷产量和厌氧消化动力学。研究结果强调了其优化沼气生产的潜力，为提高废物转化为能源的效率提供了一种可持续的战略。

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引用次数: 0

Intratumoral lung abscess from Eggerthia catenaformis in metastatic pulmonary adenocarcinoma: A case report 转移性肺腺癌伴链状卵状腺瘤内肺脓肿1例。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-03 DOI: 10.1016/j.anaerobe.2025.103018

Justin Ong , Sreekari Kedarisetti , Meenakshi Kurup , Pooja Belligund , Mohammad Al-Ajam , Aahana Gaur , Aleksey Fiksman

We present a rare case of Eggerthia catenaformis causing an intratumoral lung abscess in metastatic adenocarcinoma. Despite catheter drainage and broad-spectrum antibiotics, the infection persisted, prompting salvage intracavitary antibiotic instillation with poor response. This case highlights the diagnostic advances, therapeutic attempts, and importance of oral evaluation in anaerobic pulmonary infections.

我们报告一例罕见的链状芽孢杆菌引起转移性腺癌的瘤内肺脓肿。尽管导管引流和广谱抗生素治疗，感染仍持续存在，需要补救性腔内抗生素滴注，但反应不佳。这个病例强调了诊断的进展，治疗的尝试，以及口腔评估在厌氧肺部感染中的重要性。

引用次数: 0

Milestones in Clostridium perfringens research since 1995 1995年以来产气荚膜梭菌研究的里程碑。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-01 DOI: 10.1016/j.anaerobe.2025.103011

Bruce A. McClane , Jihong Li , Francisco A. Uzal , Julian I. Rood

Clostridium perfringens is an important human and veterinary pathogen, as well as a common member of the normal intestinal microbiota. By applying molecular approaches, substantial progress has been achieved since 1995 in understanding the pathogenicity and biology of this gram-positive, anaerobic, spore-forming bacterium. For example, there is now a much improved understanding of the structure and action of its “legacy” toxins (i.e., CPA, PFO, ETX, CPB, ITX and CPE) and the pathogenic importance of many of those toxins has now been clearly demonstrated. In addition, several new toxins have been discovered since 1995 and at least one of those new toxins (NetB) has been clearly linked to disease. The importance and diversity of mobile genetic elements, particularly conjugative plasmids, for pathogenicity and antibiotic resistance is now established. Several regulators controlling virulence gene expression have been identified and, in some cases, their regulatory mechanisms have been clarified. For some regulators, their importance for virulence has also been demonstrated. Lastly, there is also improved knowledge of sporulation and germination mechanisms for C. perfringens, as well as how sporulation contributes to disease transmission and pathogenesis for this bacterium. Despite these advances, some important questions about C. perfringens remain to be explored.

产气荚膜梭菌是一种重要的人畜致病菌，也是正常肠道菌群中常见的一员。自1995年以来，通过应用分子方法，在了解这种革兰氏阳性、厌氧、孢子形成细菌的致病性和生物学方面取得了实质性进展。例如，现在对其“遗留”毒素（即CPA， PFO， ETX， CPB， ITX和CPE）的结构和作用的了解有了很大的提高，并且许多这些毒素的致病重要性现已得到明确证明。此外，自1995年以来发现了几种新的毒素，其中至少有一种毒素（NetB）已明确与疾病有关。现已确定了可移动遗传元件，特别是共轭质粒对致病性和抗生素耐药性的重要性和多样性。已经确定了几个控制毒力基因表达的调节因子，在某些情况下，它们的调节机制已经得到澄清。对于一些监管机构来说，它们对毒性的重要性也得到了证明。最后，对产气荚膜荚膜杆菌的产孢和萌发机制，以及产孢如何促进这种细菌的疾病传播和发病机制的认识也有所提高。尽管取得了这些进展，关于产气荚膜杆菌的一些重要问题仍有待探索。

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引用次数: 0

Strain-specific genetic signatures of enterotoxigenic Bacteroides fragilis (ETBF) reveal bft-2 as a candidate biomarker for colorectal cancer 产肠毒素脆弱拟杆菌（ETBF）的菌株特异性遗传特征揭示bft-2是结直肠癌的候选生物标志物

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-01 DOI: 10.1016/j.anaerobe.2025.103008

Atefeh Rezaei , Mohammad Taheri , Mohammad Yousef Alikhani , Rasoul YousefiMashouf , Maryam Hasanzarrini , Babak Asghari

Background

The role of specific gut microbial strains in colorectal cancer (CRC) remains incompletely understood. Enterotoxigenic Bacteroides fragilis (ETBF), particularly its toxin gene (bft) isotypes, has been linked to CRC, but strain-specific contributions need further elucidation.

Materials and methods

In this case-control study, stool samples from 60 CRC patients and 60 matched healthy controls were collected at Shahid Beheshti Hospital, Hamadan, Iran (2023–2024). B. fragilis isolates were cultured and confirmed via 16S rRNA gene amplicon sequencing. The presence of bft, fpn, nanH, and leuB genes was assessed using multiplex and conventional PCR. Gene expression was quantified by qRT-PCR, and genetic diversity analyzed with REP-PCR.

Results

ETBF carriage (any bft) was significantly higher in CRC patients compared to controls (85.0 % vs. 66.7 %; p = 0.036, OR = 2.78). The bft-2 isotype was notably enriched in CRC cases (55.8 % vs. 16.7 %; p < 0.001, OR = 6.27), whereas bft-1 predominated in controls. Multiple bft alleles including rare bft-3 (always co-occurring with bft-2 in metastatic cases) were mainly found in advanced tumors. Accessory virulence genes fpn (31.4 % vs. 17.5 %; p = 0.048) and nanH (56.7 % vs. 26.7 %; p = 0.002) were also more prevalent in CRC isolates. Transcription levels of bft, fpn, and nanH increased 3.2- to 5.1-fold in CRC strains, correlating with tumor stage. No significant differences were seen for leuB. Tumor anatomical location did not affect gene prevalence or expression.

Conclusions

bft-2-positive B. fragilis strains are significantly associated with CRC, highlighting bft-2 as a promising biomarker candidate. Longitudinal and mechanistic studies are warranted to confirm its diagnostic and prognostic utility.

背景：特定肠道微生物菌株在结直肠癌（CRC）中的作用尚不完全清楚。产肠毒素的脆弱拟杆菌（ETBF），特别是其毒素基因（bft）同型型，与结直肠癌有关，但菌株特异性的贡献需要进一步阐明。材料和方法：在本病例对照研究中，收集了伊朗哈马丹Shahid Beheshti医院（2023-2024）60例结直肠癌患者和60例匹配的健康对照者的粪便样本。采用16S rRNA PCR对脆弱芽孢杆菌进行分离培养和鉴定。使用多重PCR和常规PCR评估bft、fpn、nanH和leuB基因的存在。采用qRT-PCR分析基因表达，REP-PCR分析基因多样性。结果：CRC患者的ETBF携带量（任何bft）明显高于对照组（85.0% vs. 66.7%; p=0.036， OR=2.78）。结论：bft-2阳性脆弱芽孢杆菌菌株与CRC显著相关，表明bft-2是一种有前景的生物标志物候选者。有必要进行纵向和机制研究，以证实其诊断和预后的效用。

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引用次数: 0

MALDI-TOF mass spectrometry coupled with machine learning: an accurate tool to detect toxigenic Clostridioides difficile strains MALDI-TOF质谱与机器学习相结合：一种检测产毒艰难梭菌菌株的准确工具。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-11-29 DOI: 10.1016/j.anaerobe.2025.103017

Slim Hmidi , Sophie Edouard , Jérémy Delerce , Aurélia Caputo , Carine Couderc , Simon Robinne , Pierre-Edouard Fournier , Anthony Levasseur , Hervé Chaudet , Nadim Cassir

Purpose

Clostridioides difficile infection (CDI) is one of the most common healthcare-associated infections, causing significant morbidity and mortality. A CDI diagnosis involves a clinical assessment and laboratory testing. This study aimed to evaluate the effectiveness of MALDI-TOF mass spectrometry in identifying toxigenic C. difficile strains.

Methods

From May 2019 to March 2024, all clinical samples received for C. difficile and toxins testing by PCR were also tested by culture under anaerobic conditions. At least one MALDI-TOF spectrum was used to identify all the C. difficile strains that were isolated. Whole genome sequencing was then performed to characterise the C. difficile isolates by searching for toxin genes. Machine learning and deep learning algorithms were used to distinguish between toxigenic and non-toxigenic C. difficile strains.

Results

A total of 389 C. difficile strains, isolated from the stools of 315 patients, were analyzed. Of these, 249 were toxigenic (225 ToxA + B + CDT−, 22 ToxA + B + CDT+, and 2 ToxA−B + CDT+), and 140 were non-toxigenic (ToxA−B−CDT−). The support vector machine algorithm produced the most accurate results (91.5 % accuracy and a κ value of 0.831) with high sensitivity (93.1 %) and specificity (90 %), as well as high positive and negative predictive values (PPV of 90.3 % and NPV of 92.3 %, respectively). The random forest algorithm demonstrated accuracies as high as 87.7 %.

Conclusions

Supervised machine learning paves the way for inexpensive and easy-to-use alternative methods that enable the use of MALDI-TOF MS to recognise toxigenic and non-toxigenic C. difficile strains.

目的：艰难梭菌感染（CDI）是最常见的卫生保健相关感染之一，引起显著的发病率和死亡率。CDI诊断包括临床评估和实验室检测。本研究旨在评价MALDI-TOF质谱法鉴定产毒艰难梭菌菌株的有效性。方法：2019年5月至2024年3月，对所有临床标本进行艰难梭菌和PCR毒素检测，并在厌氧条件下进行培养。使用至少一个MALDI-TOF谱来鉴定分离的所有艰难梭菌菌株。然后进行全基因组测序，通过寻找毒素基因来表征艰难梭菌分离物。使用机器学习和深度学习算法来区分产毒和非产毒艰难梭菌菌株。结果：从315例患者的粪便中分离到艰难梭菌389株。其中249例为产毒株（225例为ToxA+B+CDT-， 22例为ToxA+B+CDT+， 2例为ToxA-B+CDT+）， 140例为非产毒株（ToxA-B-CDT-）。支持向量机算法的准确率为91.5%，κ值为0.831，灵敏度为93.1%，特异度为90%，阳性预测值和阴性预测值均较高（PPV为90.3%，NPV为92.3%）。随机森林算法的准确率高达87.7%。结论：有监督的机器学习为廉价和易于使用的替代方法铺平了道路，使MALDI-TOF质谱能够识别出产毒和非产毒的艰难梭菌菌株。

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引用次数: 0

Heat-inactivated Akkermansia muciniphila AKK-PROBIO ameliorates cyclophosphamide-induced immunosuppression via the NF-κB/MAPK pathway and gut microbiota modulation 热灭活的嗜粘Akkermansia muciniphila AKK-PROBIO通过NF-κB/MAPK途径和肠道菌群调节改善环磷酰胺诱导的免疫抑制

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-11-24 DOI: 10.1016/j.anaerobe.2025.103016

Linglan Suo , Rao Li , Menghe Chen , Xin Ma , Dayong Ren , Ji Wang

Background

Immune dysfunction underlies numerous diseases. While Akkermansia muciniphila exhibits immunomodulatory potential, the efficacy of its heat-inactivated form remains incompletely characterized.

Methods

This study investigated whether oral heat-inactivated A. muciniphila AKK PROBIO alleviates cyclophosphamide (CTX)-induced immunosuppression in mice and elucidated mechanisms. Mice received CTX injections followed by oral heat-inactivated A. muciniphila for 14 days. Analyses included body/organ indices, hematology (WBC, RBC, HGB, lymphocytes), serum immunoglobulins (IgA, IgG, IgM), liver antioxidants (CAT, GSH-PX, SOD), splenic cytokines (IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ, TNF-α), immune organ histopathology, immune function (ear swelling, carbon clearance), splenic NF-κB/MAPK gene expression, gut microbiota, and short-chain fatty acids (SCFAs).

Results

Heat-inactivated A. muciniphila significantly reversed CTX-induced weight loss, hematological abnormalities, reduced immunoglobulins, and immune organ damage. It enhanced liver antioxidant activity and restored immune function (increased ear swelling, improved carbon clearance). Cytokine imbalances were corrected: suppressed cytokines (IL-1β, IL-2, IL-6, TNF-α) increased, while elevated ones (IL-4, IL-8, IFN-γ) decreased. The treatment downregulated overactivated splenic NF-κB/MAPK signaling. Gut dysbiosis was ameliorated via reduced Bacteroidota/Bacillota ratio and enrichment of beneficial taxa (e.g., o_Clostridia_UCG-014). Reduced SCFAs, particularly propanoic and isovaleric acid, were replenished.

Conclusions

In conclusion, heat-inactivated A. muciniphila AKK PROBIO effectively counteracts CTX-induced immunosuppression through multi-faceted mechanisms involving immune restoration, antioxidant enhancement, NF-κB/MAPK pathway regulation, and gut microbiota correction, highlighting its promise as a safe postbiotic therapeutic agent.

背景：免疫功能障碍是许多疾病的基础。虽然嗜粘阿克曼氏菌具有免疫调节潜能，但其热灭活形式的功效仍未完全表征。方法：研究口服热灭活A. muciniphila AKK PROBIO是否能减轻环磷酰胺（cyclophosphamide， CTX）诱导的小鼠免疫抑制，并阐明其机制。小鼠接受CTX注射，然后口服热灭活嗜粘杆菌14天。分析包括身体/器官指数、血液学（白细胞、红细胞、血红蛋白、淋巴细胞）、血清免疫球蛋白（IgA、IgG、IgM）、肝脏抗氧化剂（CAT、GSH-PX、SOD）、脾脏细胞因子（IL-1β、IL-2、IL-4、IL-6、IL-8、IL-10、IFN-γ、TNF-α）、免疫器官组织病理学、免疫功能（耳部肿胀、碳清除）、脾脏NF-κB/MAPK基因表达、肠道微生物群和短链脂肪酸（SCFAs）。结果：热灭活嗜粘杆菌显著逆转ctx诱导的体重减轻、血液学异常、免疫球蛋白减少和免疫器官损伤。它增强肝脏抗氧化活性，恢复免疫功能（增加耳肿胀，改善碳清除）。细胞因子失衡得到纠正：抑制的细胞因子（IL-1β、IL-2、IL-6、TNF-α）增加，而升高的细胞因子（IL-4、IL-8、IFN-γ）减少。治疗可下调过度激活的脾NF-κB/MAPK信号。通过降低拟杆菌/芽孢杆菌比例和丰富有益分类群（如o_Clostridia_UCG-014），肠道生态失调得到改善。减少的scfa，特别是丙酸和异戊酸，被补充。结论：热灭活的a . muciniphila AKK PROBIO通过免疫恢复、抗氧化增强、NF-κB/MAPK通路调节和肠道菌群校正等多种机制有效对抗ctx诱导的免疫抑制，是一种安全的后生物治疗药物。

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引用次数: 0

Identifying PCR-ribotype 017-lineage isolates of Clostridioides difficile using MALDI-TOF MS 利用MALDI-TOF质谱技术鉴定艰难梭菌pcr -核糖型017谱系分离物。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-11-13 DOI: 10.1016/j.anaerobe.2025.103015

Yumiko Matsuyama , Mitsutoshi Senoh , Hiroshi Miyamoto , Haru Kato

Objective

The PCR-ribotype (RT) 017-lineage of Clostridioides difficile, which is toxin A-negative, toxin B-positive, and binary toxin-negative (A⁻B⁺CDT⁻), has been endemic/epidemics particularly in Asia. In Japan, since the early 2000s, among RT017-lineage C. difficile, RT017 has been replaced by RT369, which is multidrug-resistant and has been responsible for outbreaks. We report a rapid method for identifying A⁻B⁺ C. difficile using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).

Methods

We analyzed 79 toxigenic isolates, including 37 A⁻B⁺CDT⁻ isolates and 42 isolates with other toxin profiles (A⁺B⁺CDT⁻, A⁺B⁺CDT⁺, and A⁻B⁺CDT⁺).

Results

Peaks at m/z 3289 and 3245 were detected in all 37 A⁻B⁺CDT⁻ C. difficile, including the 22 RT369 isolates, but not in 42 non-A^-B⁺CDT⁻ isolates. The method showed 100 % sensitivity and specificity in detecting A⁻B⁺CDT⁻.

Conclusions

The Identification of RT017-lineage C. difficile including RT369, which is an A⁻B⁺CDT⁻ strain causing outbreaks in Japan, by MALDI-TOF MS can be used as an additional tool to be implemented in the routine identification processes. The difference of media often used for C. difficile culture in clinical laboratories, did not affect the MALDI-TOF MS results.

目的：艰难梭菌（clostridiides difficile）的pcr -核糖型（RT） 017谱系是毒素a阴性、毒素b阳性和双毒素阴性（A-B+CDT-），特别是在亚洲流行。在日本，自21世纪初以来，在RT017谱系艰难梭菌中，RT017已被RT369所取代，RT369具有多重耐药性，是导致疫情爆发的原因。我们报道了一种快速鉴别a - b +C的方法。艰难梭菌采用基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS）。方法：对79株产毒株进行分析，包括37株A-B+CDT-毒株和42株其他毒株（A+B+CDT-、A+B+CDT+和A-B+CDT+）。结果：37例A-B+CDT-C均检测到m/z 3289和3245峰。艰难梭菌，包括22株RT369分离株，但不包括42株非a - b +CDT分离株。该方法检测A-B+CDT-的灵敏度和特异性均为100%。结论：采用MALDI-TOF质谱法鉴定包括RT369在内的rt017系艰难梭菌，可作为常规鉴定过程的补充工具。临床实验室培养艰难梭菌所用培养基的不同，对MALDI-TOF MS结果没有影响。

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引用次数: 0

Insight into the recent situation of metronidazole resistance and nim gene positivity in Bacteroides fragilis group isolates from Germany, Hungary, Italy and Slovenia 德国、匈牙利、意大利和斯洛文尼亚脆弱拟杆菌群耐甲硝唑和nim基因阳性的最新情况

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-11-13 DOI: 10.1016/j.anaerobe.2025.103014

Alida C.M. Veloo , Ádám Visnyovszki , Miriam Cordovana , Simone Ambretti , Kathleen Boiten , Arthur B. Pranada , Samo Jeverica , Károly P. Sárvári , Elisabeth Nagy , József Sóki , the ESCMID Study Group on Anaerobic Infections

We investigated metronidazole resistance mechanism in Bacteroides fragilis group strains isolated from four European countries. Among 581 isolates we identified 10 nim-positive (nimACDEH) isolates that had the genes on various plasmids or on the chromosome. The nim-positive strains harbored more cfiA genes than it could usually be detected among B. fragilis strains.

研究了来自欧洲4个国家的脆弱拟杆菌群对甲硝唑的耐药机制。在581株分离株中，我们鉴定出10株nimACDEH阳性（nimACDEH）分离株在不同的质粒或染色体上都有基因。阳性菌株携带的cfiA基因比一般脆弱芽孢杆菌中检测到的要多。

引用次数: 0

Assessing 30-day recurrence for patients testing indeterminate for Clostridioides difficile based on treatment status 基于治疗状态评估难辨梭状芽孢杆菌检测不确定患者的30天复发。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-11-04 DOI: 10.1016/j.anaerobe.2025.103012

Noah S. Ball , Benjamin Bredhold , Andrew Ward , Ryan Demkowicz , John L. Martello

Clostridioides difficile infection (CDI) multistep testing may generate indeterminate results. This single-center retrospective cohort study aims to assess recurrence of CDI in treated versus untreated adults producing indeterminate tests. We assessed 121 treated versus 49 untreated patients, finding no difference in recurrence.

艰难梭菌感染（CDI）的多步骤检测可能产生不确定的结果。本单中心回顾性队列研究旨在评估接受治疗和未接受治疗的成人CDI的复发率。我们评估了121名接受治疗的患者和49名未接受治疗的患者，发现复发无差异。

引用次数: 0

Pleural empyema due to polymicrobial Clostridioides difficile, Clostridium tertium and Robinsoniella peoriensis following C. difficile peritonitis 艰难梭菌性腹膜炎后由艰难梭菌、tertium梭菌和红棉Robinsoniella peoriensis引起的胸膜脓胸。一份病例报告。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-11-02 DOI: 10.1016/j.anaerobe.2025.103013

Edward Lovering , Obaid Haque , Marcos Wolff

We report a case of a male in his late-60s who presented with acute onset chest pain of one day duration and worsening dyspnea on exertion for more than one week, with chest radiograph notable for right-sided pleural effusion. Pleural fluid analysis revealed an exudative effusion and cultures from pleural fluid grew Clostridioides difficile, Clostridium tertium, and Robinsoniella peoriensis. His past medical history was significant for polymicrobial C. difficile and Escherichia coli peritonitis with a negative stool glutamate dehydrogenase assay. The patient was treated with six-week course of metronidazole. This is the first reported case of Robinsoniella peoriensis empyema and highlights the rarity of polymicrobial extra-intestinal anaerobic infection and the serious challenges in treatment due to the lack of established guidelines.

我们报告一个60多岁的男性病例，他表现为急性发作的胸痛，持续一天，呼吸困难加重，用力超过一周，胸片显示右侧胸腔积液。胸腔液分析显示渗出液，培养物中有艰难梭菌、tertium梭菌和peoriensis Robinsoniella。他的既往病史有多微生物艰难梭菌和大肠埃希菌腹膜炎，大便谷氨酸脱氢酶试验阴性。患者给予甲硝唑6周疗程治疗。这是第一例报道的peoriensis脓胸病例，突出了多微生物肠外厌氧感染的罕见性，以及由于缺乏既定指南而在治疗方面面临的严峻挑战。

引用次数: 0