Bifidobacterium breve is an anaerobic, gram-positive rod commonly found in the human intestinal microbiota. While rarely associated with bacteremia, most cases occur in neonates receiving B. breve-containing probiotics. Here, we report a rare case of B. breve bacteremia in an 80-year-old man presenting in cardiopulmonary arrest, despite not taking B. breve-containing probiotics. Blood cultures identified B. breve, and an 18-day course of antibiotics resolved the bacteremia. The rarity of adult cases may reflect age-related microbiota shifts and potential underdiagnosis due to the limited accuracy of rapid identification kits used in clinical practice.
{"title":"An older man with Bifidobacterium breve bacteremia, a rare condition in adults: A case report","authors":"Miku Muramatsu , Dai Akine , Teppei Sasahara , Takayuki Suzuki , Tomohiro Tamura , Takayuki Kaburagi , Shinya Watanabe","doi":"10.1016/j.anaerobe.2025.102968","DOIUrl":"10.1016/j.anaerobe.2025.102968","url":null,"abstract":"<div><div><em>Bifidobacterium breve</em> is an anaerobic, gram-positive rod commonly found in the human intestinal microbiota. While rarely associated with bacteremia, most cases occur in neonates receiving <em>B. breve</em>-containing probiotics. Here, we report a rare case of <em>B. breve</em> bacteremia in an 80-year-old man presenting in cardiopulmonary arrest, despite not taking <em>B. breve</em>-containing probiotics. Blood cultures identified <em>B. breve</em>, and an 18-day course of antibiotics resolved the bacteremia. The rarity of adult cases may reflect age-related microbiota shifts and potential underdiagnosis due to the limited accuracy of rapid identification kits used in clinical practice.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102968"},"PeriodicalIF":2.5,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-25DOI: 10.1016/j.anaerobe.2025.102967
Bruna Kelly de Oliveira Silva , José do Carmo Barbosa Neto , Laisa Gabriele Batista de Jesus , Fabrício Motteran , Marcos Antonio De Morais Jr.
The genus Fervidobacterium has only seven described species to date, namely F. changbaicum, F. gondwanense, F. islandicum, F. nodosum, F. pennivorans, F. riparium and F. thailandense. These species were first isolated from places with high thermal amplitude, such as hot springs and sites that originated from volcanic activity. With temperatures ranging from 40 °C to 90 °C, they are characteristically considered hyperthermophilic. This genus belongs to the phylum Thermotoga, composed of families of extremophilic organisms, that is, adapted to inhospitable environments. The genus is considered strictly anaerobic and heterotrophic and is widely reported in the literature regarding its biochemical machinery, as it produces a wide variety of enzymes capable of metabolising the most diverse carbon sources. It stands out for the degradation of mostly keratinolytic substrates, such as those composed of feathers of native birds. Therefore, this literature review aimed to gather information about the genus and its enzymology. There are few recent studies that outline the genus Fervidobacterium and the biotechnological and commercial potential added to the genus. The enzymes produced by these species are resistant to several detergents and organic solvents and have a high and remarkable thermal stability. Consequently, these microorganisms hold significant biotechnological potential across various industrial sectors and are also promising candidates for environmental remediation efforts.
{"title":"The genus Fervidobacterium, its thermoenzymes and biotechnological potential: an integrative review","authors":"Bruna Kelly de Oliveira Silva , José do Carmo Barbosa Neto , Laisa Gabriele Batista de Jesus , Fabrício Motteran , Marcos Antonio De Morais Jr.","doi":"10.1016/j.anaerobe.2025.102967","DOIUrl":"10.1016/j.anaerobe.2025.102967","url":null,"abstract":"<div><div>The genus <em>Fervidobacterium</em> has only seven described species to date, namely <em>F. changbaicum</em>, <em>F. gondwanense</em>, <em>F. islandicum</em>, <em>F. nodosum</em>, <em>F. pennivorans</em>, <em>F. riparium</em> and <em>F. thailandense</em>. These species were first isolated from places with high thermal amplitude, such as hot springs and sites that originated from volcanic activity. With temperatures ranging from 40 °C to 90 °C, they are characteristically considered hyperthermophilic. This genus belongs to the phylum Thermotoga, composed of families of extremophilic organisms, that is, adapted to inhospitable environments. The genus is considered strictly anaerobic and heterotrophic and is widely reported in the literature regarding its biochemical machinery, as it produces a wide variety of enzymes capable of metabolising the most diverse carbon sources. It stands out for the degradation of mostly keratinolytic substrates, such as those composed of feathers of native birds. Therefore, this literature review aimed to gather information about the genus and its enzymology. There are few recent studies that outline the genus <em>Fervidobacterium</em> and the biotechnological and commercial potential added to the genus. The enzymes produced by these species are resistant to several detergents and organic solvents and have a high and remarkable thermal stability. Consequently, these microorganisms hold significant biotechnological potential across various industrial sectors and are also promising candidates for environmental remediation efforts.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102967"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-25DOI: 10.1016/j.anaerobe.2025.102965
Wiep Klaas Smits , Kevin W. Garey , Thomas V. Riley , Stuart Johnson
{"title":"Clostridioides difficile is a bacterial priority pathogen","authors":"Wiep Klaas Smits , Kevin W. Garey , Thomas V. Riley , Stuart Johnson","doi":"10.1016/j.anaerobe.2025.102965","DOIUrl":"10.1016/j.anaerobe.2025.102965","url":null,"abstract":"","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102965"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-25DOI: 10.1016/j.anaerobe.2025.102964
Xiaoxia Zhang , Min Quan , Xin Lu , Linwan Zhang , Bing Guang , Zhiyong Zong , Xiaohui Wang
CDBN-YGXZ shows a low minimal inhibitory concentration range from 0.015 to 0.125 mg/L against clinical Clostridioides difficile strains with diverse genotypes, hardly induces bacterial resistance, and intensively inhibits 86.2 % of C. difficile adhesion to intestinal epithelial cells Caco-2. CDBN-YGXZ shows highly antibacterial activity and is potentially a candidate as a therapeutic drug.
{"title":"CDBN-YGXZ shows highly antibacterial activity against Clostridioides difficile as a potential candidate drug","authors":"Xiaoxia Zhang , Min Quan , Xin Lu , Linwan Zhang , Bing Guang , Zhiyong Zong , Xiaohui Wang","doi":"10.1016/j.anaerobe.2025.102964","DOIUrl":"10.1016/j.anaerobe.2025.102964","url":null,"abstract":"<div><div>CDBN-YGXZ shows a low minimal inhibitory concentration range from 0.015 to 0.125 mg/L against clinical <em>Clostridioides difficile</em> strains with diverse genotypes, hardly induces bacterial resistance, and intensively inhibits 86.2 % of <em>C. difficile</em> adhesion to intestinal epithelial cells Caco-2. CDBN-YGXZ shows highly antibacterial activity and is potentially a candidate as a therapeutic drug.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102964"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-24DOI: 10.1016/j.anaerobe.2025.102966
Karla Berry , Aleksandra Marek , Mairi MacLeod , Abhijit M. Bal
We investigated the management of Actinomyces breast infection. Eight different Actinomyces species were isolated from 26 patients during the study period (December 2018–November 2021). All tested isolates were susceptible (at standard or high dose) to penicillin and 75 % to clindamycin. Twenty-three patients had a history of smoking. Three had coexisting breast cancer. Prescribers need to be educated about the lack of activity of metronidazole on Actinomyces.
{"title":"An investigation into breast infections caused by Actinomyces species","authors":"Karla Berry , Aleksandra Marek , Mairi MacLeod , Abhijit M. Bal","doi":"10.1016/j.anaerobe.2025.102966","DOIUrl":"10.1016/j.anaerobe.2025.102966","url":null,"abstract":"<div><div>We investigated the management of <em>Actinomyces</em> breast infection. Eight different <em>Actinomyces</em> species were isolated from 26 patients during the study period (December 2018–November 2021). All tested isolates were susceptible (at standard or high dose) to penicillin and 75 % to clindamycin. Twenty-three patients had a history of smoking. Three had coexisting breast cancer. Prescribers need to be educated about the lack of activity of metronidazole on <em>Actinomyces</em>.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102966"},"PeriodicalIF":2.5,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-19DOI: 10.1016/j.anaerobe.2025.102962
Yukitaka Ito , Yuri Tanaka , Naomi Kaneko , Mieko Yoshida , Nobuhiko Joki
Hungatella effluvii infections in humans are rare. We report a case of H. effluvii bacteremia in a Japanese male with chronic kidney disease identified by 16S rRNA gene sequencing after initial misidentification as Clostridium species. Despite treatment, the patient died of sepsis related to a nephrostomy catheter infection. A literature review suggests that Hungatella spp. infections may be associated with severe diseases, such as sepsis and necrotizing fasciitis. This case highlights the difficulty in microbial identification, as biochemical methods were insufficient, requiring molecular biological methods. Antimicrobial susceptibility testing (AST) revealed resistance to benzylpenicillin and moxifloxacin, consistent with prior reports. This is the third documented case of H. effluvii infection, emphasizing the need for accurate pathogen identification and AST to guide optimal treatment in rare infections.
{"title":"Bacteremia due to Hungatella effluvii in a patient with end-stage renal failure requiring hemodialysis: a case report and literature review of human infections caused by Hungatella spp.","authors":"Yukitaka Ito , Yuri Tanaka , Naomi Kaneko , Mieko Yoshida , Nobuhiko Joki","doi":"10.1016/j.anaerobe.2025.102962","DOIUrl":"10.1016/j.anaerobe.2025.102962","url":null,"abstract":"<div><div><em>Hungatella effluvii</em> infections in humans are rare. We report a case of <em>H. effluvii</em> bacteremia in a Japanese male with chronic kidney disease identified by 16S rRNA gene sequencing after initial misidentification as <em>Clostridium</em> species. Despite treatment, the patient died of sepsis related to a nephrostomy catheter infection. A literature review suggests that <em>Hungatella</em> spp. infections may be associated with severe diseases, such as sepsis and necrotizing fasciitis. This case highlights the difficulty in microbial identification, as biochemical methods were insufficient, requiring molecular biological methods. Antimicrobial susceptibility testing (AST) revealed resistance to benzylpenicillin and moxifloxacin, consistent with prior reports. This is the third documented case of <em>H. effluvii</em> infection, emphasizing the need for accurate pathogen identification and AST to guide optimal treatment in rare infections.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102962"},"PeriodicalIF":2.5,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.anaerobe.2025.102960
Jourdan E. Lakes , Alexander W. Altman , Mark A. Berhow , Isabelle A. Kagan , T.G. Nagaraja , David L. Harmon , Michael D. Flythe
Fusobacterium necrophorum commonly causes liver abscesses in grain-finished beef, which is mitigated using antibiotics in feed. However, microbial resistance development necessitates exploration of antibiotic alternatives. Here we report the antimicrobial efficacy of Cannabis sativa L. (hemp) crude oil extract and its constituents on Fusobacterium species.
{"title":"In vitro antimicrobial efficacy of Cannabis sativa L. (hemp) crude oil extract on Fusobacterium necrophorum of bovine origin","authors":"Jourdan E. Lakes , Alexander W. Altman , Mark A. Berhow , Isabelle A. Kagan , T.G. Nagaraja , David L. Harmon , Michael D. Flythe","doi":"10.1016/j.anaerobe.2025.102960","DOIUrl":"10.1016/j.anaerobe.2025.102960","url":null,"abstract":"<div><div><em>Fusobacterium necrophorum</em> commonly causes liver abscesses in grain-finished beef, which is mitigated using antibiotics in feed. However, microbial resistance development necessitates exploration of antibiotic alternatives. Here we report the antimicrobial efficacy of <em>Cannabis sativa</em> L. (hemp) crude oil extract and its constituents on <em>Fusobacterium</em> species.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102960"},"PeriodicalIF":2.5,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-15DOI: 10.1016/j.anaerobe.2025.102959
Lengsea Eng , Kefyalew Addis Alene , Deirdre A. Collins , Su-Chen Lim , Viso Srey , Choeung Chea , Sotera Yohn , Setha Leng , Archie C.A. Clements , Thomas V. Riley
Background
Children may play an important role in disseminating Clostridioides (Clostridium) difficile within hospital and community settings. In many parts of the world, there has been a recent increase in interest in C. difficile infection (CDI) in paediatric populations.
Objectives
This study aimed to investigate the prevalence of, risk factors for, and molecular types of C. difficile in hospitalised children in Cambodia.
Methods
Stool samples were collected from children at the National Paediatric Hospital in Phnom Penh, Cambodia, between June 2022 and March 2023, for C. difficile culture. Toxin gene PCR and PCR ribotyping were performed on all isolates.
Results
Of 122 hospitalised children recruited, C. difficile was identified in 47 (38.5 %). Toxigenic strains accounted for 25.5 % (12/47), with ribotype (RT) 012 the most predominant (7/12), followed by RTs 014/020 (3/12) and 017 (1/12). Non-toxigenic strains were dominated by RTs QX011, QX675 and 009/QX107. Novel strains represented 70 % (33/47) of isolates. Significant risk factors included antimicrobial use in the week before detection (OR = 5.15; 95 %CI: 1.125–21.21) and current use of other medications (OR = 13.02, 95 %CI: 1.32–128.67). Diarrhoea and abdominal pain were negatively associated with the presence of C. difficile.
Conclusions
A high prevalence of C. difficile was found in hospitalised children, with a high proportion of non-toxigenic and novel strains. Larger studies are required with whole genome sequencing necessary for characterising novel strains and advancing molecular epidemiological research in Asia.
{"title":"Clostridioides (Clostridium) difficile in hospitalised children in Cambodia","authors":"Lengsea Eng , Kefyalew Addis Alene , Deirdre A. Collins , Su-Chen Lim , Viso Srey , Choeung Chea , Sotera Yohn , Setha Leng , Archie C.A. Clements , Thomas V. Riley","doi":"10.1016/j.anaerobe.2025.102959","DOIUrl":"10.1016/j.anaerobe.2025.102959","url":null,"abstract":"<div><h3>Background</h3><div>Children may play an important role in disseminating <em>Clostridioides</em> (<em>Clostridium</em>) <em>difficile</em> within hospital and community settings. In many parts of the world, there has been a recent increase in interest in <em>C. difficile</em> infection (CDI) in paediatric populations.</div></div><div><h3>Objectives</h3><div>This study aimed to investigate the prevalence of, risk factors for, and molecular types of <em>C. difficile</em> in hospitalised children in Cambodia.</div></div><div><h3>Methods</h3><div>Stool samples were collected from children at the National Paediatric Hospital in Phnom Penh, Cambodia, between June 2022 and March 2023, for <em>C. difficile</em> culture. Toxin gene PCR and PCR ribotyping were performed on all isolates.</div></div><div><h3>Results</h3><div>Of 122 hospitalised children recruited, <em>C. difficile</em> was identified in 47 (38.5 %). Toxigenic strains accounted for 25.5 % (12/47), with ribotype (RT) 012 the most predominant (7/12), followed by RTs 014/020 (3/12) and 017 (1/12). Non-toxigenic strains were dominated by RTs QX011, QX675 and 009/QX107. Novel strains represented 70 % (33/47) of isolates. Significant risk factors included antimicrobial use in the week before detection (OR = 5.15; 95 %CI: 1.125–21.21) and current use of other medications (OR = 13.02, 95 %CI: 1.32–128.67). Diarrhoea and abdominal pain were negatively associated with the presence of <em>C. difficile.</em></div></div><div><h3>Conclusions</h3><div>A high prevalence of <em>C. difficile</em> was found in hospitalised children, with a high proportion of non-toxigenic and novel strains. Larger studies are required with whole genome sequencing necessary for characterising novel strains and advancing molecular epidemiological research in Asia.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102959"},"PeriodicalIF":2.5,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was to characterize and determine the classification of the novel strain TM49T, isolated from a blood culture of a patient who developed bacteremia while receiving high-dose cytarabine chemotherapy for acute myeloid leukemia.
Methods
The novel strain TM49T was characterized at the species level through a polyphasic taxonomic analysis, incorporating morphological, physiological, biochemical, phylogenetic, and genome sequence analyses.
Results
16S rRNA gene sequence analysis revealed that the strain belonged to the genus Lachnoanaerobaculum. The 16S rRNA gene sequence of strain TM49T was observed to be most similar to that of "Lachnoanaerobaculum gingivalis" strain ChDC B114T (99.2 %). Strain TM49T formed circular, glossy, slightly milky colonies with an undulate margin on BD™ Columbia Agar containing 5 % sheep blood after 48 h at 37 °C, exhibiting less spreading compared to species of the genus Lachnoanaerobaculum. The major fatty acids of strain TM49T were C16:0, C14:0, C18:1 ω9c DMA, and C18:1 ω7c DMA. The draft genome sequence of strain TM49T was 2,821,554 bp in length, with a G + C content of 36.4. Digital DNA-DNA hybridization values between strain TM49T and “L. gingivalis” ChDC B114T, L. umeaense CD3: 22T, L. orale N1T, and L. saburreum CCUG 28089T were 52.2 %, 29.7 %, 26.2 %, and 24.5 %, respectively. The Orthologous Average Nucleotide Identity values between strain TM49T and “L. gingivalis” ChDC B114T, L. umeaense CD3: 22T, L. orale N1T, and L. saburreum CCUG 28089T were 93.1 %, 84.9 %, 83.2 %, and 81.5 %, respectively.
Conclusion
Strain TM49T (JCM 36186T = DSM 116945T) was found to be distinct from previously described species of the genus Lachnoanaerobaculum. Therefore, it is proposed as a new species, Lachnoanaerobaculum sanguinis sp. Nov., with strain TM49T as the type strain (= JCM 36186T = DSM 116945T).
{"title":"Characterization of Lachnoanaerobaculum sanguinis sp. nov., isolated from a blood culture of an acute myeloid leukemia patient with chemotherapy-related bacteremia","authors":"Yumi Suenari , Toshiyuki Matsubara , Yuka Hiroshima , Muhammad Reza Pahlevi , Hiroka Shimada , Yuto Ochi , Naoto Okada , Masami Sato , Hideki Fujii , Tomoko Sumitomo , Hidehiro Kishimoto , Akikazu Murakami","doi":"10.1016/j.anaerobe.2025.102961","DOIUrl":"10.1016/j.anaerobe.2025.102961","url":null,"abstract":"<div><h3>Objective</h3><div>This study was to characterize and determine the classification of the novel strain TM49<sup>T</sup>, isolated from a blood culture of a patient who developed bacteremia while receiving high-dose cytarabine chemotherapy for acute myeloid leukemia.</div></div><div><h3>Methods</h3><div>The novel strain TM49<sup>T</sup> was characterized at the species level through a polyphasic taxonomic analysis, incorporating morphological, physiological, biochemical, phylogenetic, and genome sequence analyses.</div></div><div><h3>Results</h3><div>16S rRNA gene sequence analysis revealed that the strain belonged to the genus <em>Lachnoanaerobaculum</em>. The 16S rRNA gene sequence of strain TM49<sup>T</sup> was observed to be most similar to that of \"<em>Lachnoanaerobaculum gingivalis</em>\" strain ChDC B114<sup>T</sup> (99.2 %). Strain TM49<sup>T</sup> formed circular, glossy, slightly milky colonies with an undulate margin on BD™ Columbia Agar containing 5 % sheep blood after 48 h at 37 °C, exhibiting less spreading compared to species of the genus <em>Lachnoanaerobaculum</em>. The major fatty acids of strain TM49<sup>T</sup> were C<sub>16:0</sub>, C<sub>14:0</sub>, C<sub>18:1</sub> ω9c DMA, and C<sub>18:1</sub> ω7c DMA. The draft genome sequence of strain TM49<sup>T</sup> was 2,821,554 bp in length, with a G + C content of 36.4. Digital DNA-DNA hybridization values between strain TM49<sup>T</sup> and “<em>L. gingivalis</em>” ChDC B114<sup>T</sup>, <em>L. umeaense</em> CD3: 22<sup>T</sup>, <em>L. orale</em> N1<sup>T</sup>, and <em>L. saburreum</em> CCUG 28089<sup>T</sup> were 52.2 %, 29.7 %, 26.2 %, and 24.5 %, respectively. The Orthologous Average Nucleotide Identity values between strain TM49<sup>T</sup> and “<em>L. gingivalis</em>” ChDC B114<sup>T</sup>, <em>L. umeaense</em> CD3: 22<sup>T</sup>, <em>L. orale</em> N1<sup>T</sup>, and <em>L. saburreum</em> CCUG 28089<sup>T</sup> were 93.1 %, 84.9 %, 83.2 %, and 81.5 %, respectively.</div></div><div><h3>Conclusion</h3><div>Strain TM49<sup>T</sup> (JCM 36186<sup>T</sup> = DSM 116945<sup>T</sup>) was found to be distinct from previously described species of the genus <em>Lachnoanaerobaculum</em>. Therefore, it is proposed as a new species, <em>Lachnoanaerobaculum sanguinis</em> sp. Nov., with strain TM49<sup>T</sup> as the type strain (= JCM 36186<sup>T</sup> = DSM 116945<sup>T</sup>).</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102961"},"PeriodicalIF":2.5,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.anaerobe.2025.102958
A. Arun Prince Milton , Torik Basar , K. Srinivas , Aleimo G. Momin , Sabia Khan , G. Bhuvana Priya , Samir Das , S. Ghatak , K. Puro
In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the etx gene, which encodes the epsilon toxin of Clostridium perfringens. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.
{"title":"Novel competitive annealing-mediated isothermal amplification (CAMP)-based detection of epsilon toxin gene in Clostridium perfringens","authors":"A. Arun Prince Milton , Torik Basar , K. Srinivas , Aleimo G. Momin , Sabia Khan , G. Bhuvana Priya , Samir Das , S. Ghatak , K. Puro","doi":"10.1016/j.anaerobe.2025.102958","DOIUrl":"10.1016/j.anaerobe.2025.102958","url":null,"abstract":"<div><div>In this study, we developed a novel competitive annealing-mediated isothermal amplification (CAMP)-based assay for the detection of the <em>etx</em> gene, which encodes the epsilon toxin of <em>Clostridium perfringens</em>. The assay, performed at 60 °C for 60 min, demonstrated 100 % specificity and was 100 times more sensitive than endpoint PCR.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"93 ","pages":"Article 102958"},"PeriodicalIF":2.5,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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