Anaerobe最新文献_第5页

Assessing 30-day recurrence for patients testing indeterminate for Clostridioides difficile based on treatment status 基于治疗状态评估难辨梭状芽孢杆菌检测不确定患者的30天复发。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-01 Epub Date: 2025-11-04 DOI: 10.1016/j.anaerobe.2025.103012

Noah S. Ball , Benjamin Bredhold , Andrew Ward , Ryan Demkowicz , John L. Martello

Clostridioides difficile infection (CDI) multistep testing may generate indeterminate results. This single-center retrospective cohort study aims to assess recurrence of CDI in treated versus untreated adults producing indeterminate tests. We assessed 121 treated versus 49 untreated patients, finding no difference in recurrence.

艰难梭菌感染（CDI）的多步骤检测可能产生不确定的结果。本单中心回顾性队列研究旨在评估接受治疗和未接受治疗的成人CDI的复发率。我们评估了121名接受治疗的患者和49名未接受治疗的患者，发现复发无差异。

引用次数: 0

Current practices to reduce the risk of transmission of Clostridioides difficile in France: results of a national survey 在法国减少艰难梭菌传播风险的现行做法：一项全国调查的结果。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-01 Epub Date: 2025-10-09 DOI: 10.1016/j.anaerobe.2025.103004

Nagham Khanafer , Anne-Gaëlle Venier , Raymond Nasso , Romane Baroux , Philippe Vanhems

Introduction

Clostridioides difficile is a major cause of healthcare-associated infections. In healthcare settings, interventions like contact precautions (CP), hand hygiene with soap and water, sporicidal environmental cleaning, and antimicrobial stewardship, are essential to reducing the risk C. difficile transmission. This study aimed to evaluate infection prevention and control (IPC) measures targeting C. difficile infection (CDI) in French hospitals.

Materials and methods

An anonymous online survey was conducted from August to December 2022. The assessed CDI-related IPC practices. Descriptive analysis was performed using IBM SPSS.

Results

A total of 350 professionals from all French regions completed the survey; 70.3 % were members of an infection control team members. CP for symptomatic patients were implemented by 70 %, and continued ≥48 h after diarrhea resolution of by 49 %; 4.9 % continued CP until discharge. Gloves, gowns, and aprons were worn before room entry by 62 %, 55 % and 21 % respectively. Hand hygiene with soap and water followed by alcohol-based hand rubs was reported by 85 %. Daily surface cleaning of rooms, toilets and floors was reported by 98 %, 96 %, and 87 % respectively. Cleaning was performed using sporicidal products (71 %), chlorine-containing agents (23 %), or non-sporicidal agents (5 %).

Conclusion

While key IPC measures are widely acknowledged, their implementation varies. Broader studies are needed to evaluate their effectiveness and address inconsistencies.

简介：艰难梭菌是卫生保健相关感染的主要原因。在卫生保健环境中，接触预防措施、用肥皂和水洗手、杀孢环境清洁和抗菌管理等干预措施对于降低艰难梭菌传播风险至关重要。本研究旨在评价法国医院针对艰难梭菌感染（CDI）的感染预防和控制（IPC）措施。材料与方法：于2022年8月至12月进行匿名在线调查。评估的cdi相关IPC实践。采用IBM SPSS进行描述性分析。结果：共有350名来自法国各地区的专业人士完成了调查；70.3%为感染控制小组成员；对有症状患者实施CP的比例为70%，腹泻消退后持续≥48 h的比例为49%；4.9%继续CP直到出院。进入房间前戴手套、穿长袍和围裙的比例分别为62%、55%和21%。85%的人用肥皂和水洗手，然后用含酒精的洗手液洗手。房间、厕所和地板的每日表面清洁率分别为98%、96%和87%。使用杀孢剂产品（71%）、含氯剂（23%）或非杀孢剂（5%）进行清洁。结论：虽然关键的IPC措施得到广泛认可，但其实施情况各不相同。需要更广泛的研究来评估其有效性并解决不一致之处。

{"title":"Current practices to reduce the risk of transmission of Clostridioides difficile in France: results of a national survey","authors":"Nagham Khanafer , Anne-Gaëlle Venier , Raymond Nasso , Romane Baroux , Philippe Vanhems","doi":"10.1016/j.anaerobe.2025.103004","DOIUrl":"10.1016/j.anaerobe.2025.103004","url":null,"abstract":"<div><h3>Introduction</h3><div><em>Clostridioides difficile</em> is a major cause of healthcare-associated infections. In healthcare settings, interventions like contact precautions (CP), hand hygiene with soap and water, sporicidal environmental cleaning, and antimicrobial stewardship, are essential to reducing the risk <em>C. difficile</em> transmission. This study aimed to evaluate infection prevention and control (IPC) measures targeting <em>C. difficile</em> infection (CDI) in French hospitals.</div></div><div><h3>Materials and methods</h3><div>An anonymous online survey was conducted from August to December 2022. The assessed CDI-related IPC practices. Descriptive analysis was performed using IBM SPSS.</div></div><div><h3>Results</h3><div>A total of 350 professionals from all French regions completed the survey; 70.3 % were members of an infection control team members. CP for symptomatic patients were implemented by 70 %, and continued ≥48 h after diarrhea resolution of by 49 %; 4.9 % continued CP until discharge. Gloves, gowns, and aprons were worn before room entry by 62 %, 55 % and 21 % respectively. Hand hygiene with soap and water followed by alcohol-based hand rubs was reported by 85 %. Daily surface cleaning of rooms, toilets and floors was reported by 98 %, 96 %, and 87 % respectively. Cleaning was performed using sporicidal products (71 %), chlorine-containing agents (23 %), or non-sporicidal agents (5 %).</div></div><div><h3>Conclusion</h3><div>While key IPC measures are widely acknowledged, their implementation varies. Broader studies are needed to evaluate their effectiveness and address inconsistencies.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"96 ","pages":"Article 103004"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in understanding Cutibacterium acnes hemolysis: virulence factor or genomic trait? 了解痤疮表皮杆菌溶血的进展：毒力因子还是基因组性状？

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-12-01 Epub Date: 2025-09-30 DOI: 10.1016/j.anaerobe.2025.103003

L. Ruffier d’Epenoux , E. Fayoux , K. Rwayane , C. Hervochon , S. Corvec

Background

Cutibacterium acnes is now recognized a major opportunistic pathogen causing a wide range of infections. Henceforth, this bacterium is largely involved in device-related infections especially prosthetic joint infections, spine infections but also cardiovascular infections, neurosurgical implant infections and breast infections. It has also been associated with acne and probably sarcoidosis and prostate tumors. We conducted a narrative review to better understand the phylotypes involved in these clinical entities and if hemolysis can be a diagnosis maker of pathogenicity.

Methods

We searched the PubMed database for English language articles related to C. acnes phylotype and clinical entities and hemolysin characteristic.

Results

As a successful commensal microorganism, C. acnes produces many virulence factors including cytolysins that most likely facilitate tissue damages due to its β-hemolytic trait. In this review, we summarize its involvement in different clinical settings and the role of this virulence factor depending on the phylotype implicated. Our analysis of the literature on C. acnes β-hemolysis clinical strains sheds new light on its potential impact in distinguishing C. acnes infection strains from contaminant strains.

Conclusion

The litterature and our results demonstrate the genetic nature of the β-hemolysis in C. acnes strains rather than being a mean to differentiate virulent clinical strains from commensal or other strains.

背景：痤疮表皮杆菌是目前公认的引起广泛感染的主要条件致病菌。此后，这种细菌在很大程度上涉及与器械相关的感染，特别是假体关节感染、脊柱感染，但也涉及心血管感染、神经外科植入物感染和乳房感染。它还与痤疮、结节病和前列腺肿瘤有关。我们进行了一项叙述性回顾，以更好地了解这种临床实体所涉及的种型，以及溶血是否可以作为致病性的诊断因素。方法：我们检索PubMed数据库中与痤疮C.系统类型、临床实体和溶血素特征相关的英文文章。结果：作为一种成功的共生微生物，痤疮C.产生包括溶细胞素在内的许多毒力因子，由于其β-溶血特性，这些毒力因子很可能导致组织损伤。在这篇综述中，我们总结了它在不同临床环境中的作用，以及根据所涉及的种型，这种毒力因子的作用。我们对痤疮C. β-溶血临床菌株的文献分析揭示了其在区分痤疮C.感染菌株和污染菌株方面的潜在影响。结论：文献和我们的结果表明，痤疮C.菌株中β-溶血的遗传性质，而不是区分毒性临床菌株与共生菌株或其他菌株的一种手段。

{"title":"Advances in understanding Cutibacterium acnes hemolysis: virulence factor or genomic trait?","authors":"L. Ruffier d’Epenoux , E. Fayoux , K. Rwayane , C. Hervochon , S. Corvec","doi":"10.1016/j.anaerobe.2025.103003","DOIUrl":"10.1016/j.anaerobe.2025.103003","url":null,"abstract":"<div><h3>Background</h3><div><em>Cutibacterium acnes</em> is now recognized a major opportunistic pathogen causing a wide range of infections. Henceforth, this bacterium is largely involved in device-related infections especially prosthetic joint infections, spine infections but also cardiovascular infections, neurosurgical implant infections and breast infections. It has also been associated with acne and probably sarcoidosis and prostate tumors. We conducted a narrative review to better understand the phylotypes involved in these clinical entities and if hemolysis can be a diagnosis maker of pathogenicity.</div></div><div><h3>Methods</h3><div>We searched the PubMed database for English language articles related to <em>C. acnes</em> phylotype and clinical entities and hemolysin characteristic.</div></div><div><h3>Results</h3><div>As a successful commensal microorganism, <em>C. acnes</em> produces many virulence factors including cytolysins that most likely facilitate tissue damages due to its β-hemolytic trait. In this review, we summarize its involvement in different clinical settings and the role of this virulence factor depending on the phylotype implicated. Our analysis of the literature on <em>C. acnes</em> β-hemolysis clinical strains sheds new light on its potential impact in distinguishing <em>C. acnes</em> infection strains from contaminant strains.</div></div><div><h3>Conclusion</h3><div>The litterature and our results demonstrate the genetic nature of the β-hemolysis in <em>C. acnes</em> strains rather than being a mean to differentiate virulent clinical strains from commensal or other strains.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"96 ","pages":"Article 103003"},"PeriodicalIF":2.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145211394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of Clostridioides difficile in retail garden products from two Australian states. 艰难梭菌在澳大利亚两个州零售园艺产品中的高流行率。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-07-25 DOI: 10.1016/j.anaerobe.2025.102987

Jessica M Chisholm, Charlotte Bell, Molly Lattin, Su Chen Lim, Simon A Reid, Linda A Selvey, Thomas V Riley

Background: Despite historically being considered a healthcare-associated pathogen, rates of Clostridioides difficile infection in the community have risen greatly in recent decades. As a pathogen of One Health importance, sources of C. difficile in the community have been sought to understand and control community-associated C. difficile infection transmission.

Objectives: This study aimed to investigate the prevalence and molecular characteristics of C. difficile isolated from retail garden products in two Australian states.

Methods: Soil conditioners, soil mixes and turf samples were collected from commercial garden product suppliers in Western Australia and Queensland from May 2023 to January 2024. Following enrichment culture, PCR ribotyping and toxin gene profiling were performed on C. difficile isolates.

Results: C. difficile was recovered from 257 of 479 (53.7 %) retail garden products in this study. Prevalence was highest in turf (67.1 %, 53/79), followed by soil mixes (57.1 %, 148/259) and soil conditioners (39.7 %, 56/141). Of the 113 distinct ribotypes identified, the most common were 014/020 and QX 686. Toxigenic ribotypes associated with both human infection and production animals in Australia, including 014/020, 056 and 087, were recovered.

Conclusion: A high prevalence of C. difficile was found in garden products sold to consumers, including toxigenic strains associated with C. difficile infection in humans and animals. More discriminatory characterisation of isolates is required to determine if retail garden products contribute to the burden of community-associated C. difficile infection in Australia.

背景：尽管历史上被认为是一种与卫生保健相关的病原体，但近几十年来，艰难梭菌在社区中的感染率大幅上升。作为一种具有“同一个健康”重要性的病原体，人们一直在寻求社区中艰难梭菌的来源，以了解和控制社区相关的艰难梭菌感染传播。目的：本研究旨在调查澳大利亚两个州零售园艺产品中分离的艰难梭菌的流行率和分子特征。方法：于2023年5月至2024年1月在西澳大利亚州和昆士兰州的商业园艺产品供应商处采集土壤调理剂、土壤混合料和草坪样品。富集培养后，对难辨梭菌分离株进行PCR核糖分型和毒素基因谱分析。结果：从479种零售园艺产品中检出艰难梭菌257种（53.7%）。草皮患病率最高（67.1%,53/79），其次是混合土（57.1%,148/259）和土壤调理剂（39.7%,56/141）。在鉴定出的113种不同的核型中，最常见的是014/020和QX 686。在澳大利亚发现了与人类感染和生产动物相关的产毒核型，包括014/020、056和087。结论：在销售给消费者的园艺产品中发现艰难梭菌的高发率，包括与人类和动物中艰难梭菌感染相关的产毒菌株。需要对分离株进行更具歧视性的特征描述，以确定零售园艺产品是否会导致澳大利亚社区相关艰难梭菌感染的负担。

{"title":"High prevalence of Clostridioides difficile in retail garden products from two Australian states.","authors":"Jessica M Chisholm, Charlotte Bell, Molly Lattin, Su Chen Lim, Simon A Reid, Linda A Selvey, Thomas V Riley","doi":"10.1016/j.anaerobe.2025.102987","DOIUrl":"10.1016/j.anaerobe.2025.102987","url":null,"abstract":"<p><strong>Background: </strong>Despite historically being considered a healthcare-associated pathogen, rates of Clostridioides difficile infection in the community have risen greatly in recent decades. As a pathogen of One Health importance, sources of C. difficile in the community have been sought to understand and control community-associated C. difficile infection transmission.</p><p><strong>Objectives: </strong>This study aimed to investigate the prevalence and molecular characteristics of C. difficile isolated from retail garden products in two Australian states.</p><p><strong>Methods: </strong>Soil conditioners, soil mixes and turf samples were collected from commercial garden product suppliers in Western Australia and Queensland from May 2023 to January 2024. Following enrichment culture, PCR ribotyping and toxin gene profiling were performed on C. difficile isolates.</p><p><strong>Results: </strong>C. difficile was recovered from 257 of 479 (53.7 %) retail garden products in this study. Prevalence was highest in turf (67.1 %, 53/79), followed by soil mixes (57.1 %, 148/259) and soil conditioners (39.7 %, 56/141). Of the 113 distinct ribotypes identified, the most common were 014/020 and QX 686. Toxigenic ribotypes associated with both human infection and production animals in Australia, including 014/020, 056 and 087, were recovered.</p><p><strong>Conclusion: </strong>A high prevalence of C. difficile was found in garden products sold to consumers, including toxigenic strains associated with C. difficile infection in humans and animals. More discriminatory characterisation of isolates is required to determine if retail garden products contribute to the burden of community-associated C. difficile infection in Australia.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102987"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deletion of rgaS reduces virulence in a mouse model of Clostridioides difficile infection 在艰难梭菌感染小鼠模型中，缺失rgaS可降低毒力。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-08-09 DOI: 10.1016/j.anaerobe.2025.102988

Terry W. Bilverstone , Morgan E. Simpson , Nicholas Hagspiel , William A. Petri , Sarah A. Kuehne , Nigel P. Minton

Two-component system signalling is involved in several processes relevant to Clostridioides difficile virulence. We demonstrate that deletion of the orphan histidine kinase rgaS (R20291_0503), reduces detectable toxin in the supernatant of R20291 and impairs virulence in a mouse model of infection. These data suggest that RgaS regulates virulence capabilities in hypervirulent C. difficile.

双组分系统信号参与了艰难梭菌毒力相关的几个过程。我们证明，缺失孤儿组氨酸激酶rgaS (R20291_0503)，减少了R20291上清中可检测到的毒素，并削弱了小鼠感染模型的毒力。这些数据表明，RgaS调节了高毒力艰难梭菌的毒力能力。

引用次数: 0

High prevalence and clonality of Clostridioides difficile isolates from cryptic clades in household composters and small mammals 难辨梭状芽孢杆菌在家庭堆肥和小型哺乳动物中的高流行率和克隆性。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-08-18 DOI: 10.1016/j.anaerobe.2025.102990

Klemen Trsinar , Sabina Mlakar , Franc Janzekovic , Maja Rupnik , Sandra Janezic

Objective

We aimed to investigate the prevalence, genotypic diversity, and clonality of Clostridioides difficile strains from household composters and faeces of small mammals captured nearby.

Methods

Compost piles from three locations were sampled, with three compost samples collected from each bin. Additionally, 5–6 small mammals were trapped around each compost pile. C. difficile was isolated from faecal and compost samples and characterized with PCR ribotyping, toxinotyping, and genome sequencing.

Results

C. difficile was detected in all nine compost samples, while only two (12.5 %) of 16 small mammal faecal samples tested positive. Ten PCR ribotypes (RTs) were identified, with only one, RT014/020, being toxigenic. The remaining nine RTs belonged to two cryptic clades, C-II and C-III. Some of the isolates from cryptic clades i.e. RT SLO 308 carried a divergent tcdA gene and yielded weakly positive results with C. diff Quik Chek Complete immunoassay. Clonal C. difficile isolates of RT SLO 308 were found at the same site in both a compost sample and a small mammal, suggesting potential transmission. Clonality was also observed among isolates from different compost samples within a single pile.

Conclusions

While our study could not establish the exact direction of C. difficile transmission between compost and mice, it highlights the role of small mammals within the One Health framework of C. difficile transmission pathways.

目的：调查家庭堆肥和附近捕获的小兽粪便中艰难梭菌的流行率、基因型多样性和克隆性。方法：对3个地点的堆肥堆进行取样，每箱取3个堆肥样。此外，在每个堆肥堆周围捕获了5-6只小型哺乳动物。从粪便和堆肥样本中分离出艰难梭菌，并通过PCR核糖分型、毒素分型和基因组测序对其进行了鉴定。结果：9份堆肥样品均检出艰难梭菌，16份小型哺乳动物粪便样品中仅有2份（12.5%）检出阳性。共鉴定出10个PCR核糖型（rt），其中只有1个（RT014/020）为产毒核糖型。其余9个RTs属于两个隐枝，C-II和C-III。部分隐匿分支（如RT SLO 308）的分离株携带不同的tcdA基因，其快速免疫检测结果呈弱阳性。在堆肥样品和小型哺乳动物中均发现难辨梭菌SLO 308克隆分离株，提示可能存在传播。从不同堆肥样品中分离的菌株在同一堆中也观察到克隆性。结论：虽然我们的研究不能确定艰难梭菌在堆肥和小鼠之间传播的确切方向，但它突出了小型哺乳动物在One Health框架下艰难梭菌传播途径的作用。

{"title":"High prevalence and clonality of Clostridioides difficile isolates from cryptic clades in household composters and small mammals","authors":"Klemen Trsinar , Sabina Mlakar , Franc Janzekovic , Maja Rupnik , Sandra Janezic","doi":"10.1016/j.anaerobe.2025.102990","DOIUrl":"10.1016/j.anaerobe.2025.102990","url":null,"abstract":"<div><h3>Objective</h3><div>We aimed to investigate the prevalence, genotypic diversity, and clonality of <em>Clostridioides difficile</em> strains from household composters and faeces of small mammals captured nearby.</div></div><div><h3>Methods</h3><div>Compost piles from three locations were sampled, with three compost samples collected from each bin. Additionally, 5–6 small mammals were trapped around each compost pile. <em>C. difficile</em> was isolated from faecal and compost samples and characterized with PCR ribotyping, toxinotyping, and genome sequencing.</div></div><div><h3>Results</h3><div><em>C. difficile</em> was detected in all nine compost samples, while only two (12.5 %) of 16 small mammal faecal samples tested positive. Ten PCR ribotypes (RTs) were identified, with only one, RT014/020, being toxigenic. The remaining nine RTs belonged to two cryptic clades, C-II and C-III. Some of the isolates from cryptic clades i.e. RT SLO 308 carried a divergent <em>tcd</em>A gene and yielded weakly positive results with <em>C. diff</em> Quik Chek Complete immunoassay. Clonal <em>C. difficile</em> isolates of RT SLO 308 were found at the same site in both a compost sample and a small mammal, suggesting potential transmission. Clonality was also observed among isolates from different compost samples within a single pile.</div></div><div><h3>Conclusions</h3><div>While our study could not establish the exact direction of <em>C. difficile</em> transmission between compost and mice, it highlights the role of small mammals within the One Health framework of <em>C. difficile</em> transmission pathways.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102990"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolism of Lactobacillus and Gardnerella vaginalis in vaginal defined media 乳杆菌和阴道加德纳菌在阴道介质中的代谢

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-08-22 DOI: 10.1016/j.anaerobe.2025.102991

Victoria Horrocks , Charlotte K. Hind , J. Mark Sutton , Rachel M. Tribe , A. James Mason

Objectives

This study evaluates how well a vaginal defined medium (VDM) replicates the in vivo metabolic behaviour of key vaginal microbiota members - Lactobacillus crispatus, L. jensenii, and diverse Gardnerella vaginalis isolates - compared to brain heart infusion (BHI) medium.

Methods

We used ¹H NMR spectroscopy to characterise metabolic profiles during in vitro growth of Lactobacillus and Gardnerella species in VDM and BHI. Differences in metabolite production, growth, acidification, and carbohydrate utilisation were assessed.

Results

Both L. crispatus and L. jensenii grow well in VDM, produce substantially more lactate than in BHI, and acidify the culture more strongly - better reflecting the low pH environment of Lactobacillus-dominant vaginal microbiota. In contrast, G. vaginalis grows less robustly in VDM than in BHI, though key metabolic traits such as the Bifidobacterium shunt and mixed acid fermentation (evidenced by formate production) are preserved. Notably, neither genus consume available glucose, yet still ferment carbohydrates, suggesting a metabolic preference for glycogen over glucose. Evidence of glucose release further indicates glycogen breakdown in culture.

Conclusions

VDM more accurately models the metabolic activity and environmental effects of vaginal Lactobacillus species than BHI, particularly in terms of acidification and lactate production. Although G. vaginalis growth is limited in VDM, its characteristic metabolic pathways remain evident. These findings underscore the value of VDM in modelling key metabolic features of the vaginal microbiota, especially under conditions where Lactobacillus dominate or Gardnerella is prevalent.

目的：本研究评估阴道定义培养基（VDM）与脑心灌注（BHI）培养基相比，如何很好地复制关键阴道微生物群成员的体内代谢行为- crispatus乳杆菌，L. jensenii和多种阴道加德纳菌分离株。方法采用核磁共振（1H NMR）技术对乳酸菌和加德纳菌在体外生长过程中的代谢谱进行表征。评估了代谢物产生、生长、酸化和碳水化合物利用的差异。结果crispatus和L. jensenii在VDM中生长良好，产生的乳酸含量明显高于BHI，并且对培养物的酸化作用更强，更好地反映了乳酸菌优势阴道微生物群的低pH环境。相比之下，阴道弧菌在VDM中的生长不如在BHI中的健壮，尽管双歧杆菌分流和混合酸发酵等关键代谢特征（由甲酸产生证明）得以保留。值得注意的是，这两个属都不消耗可利用的葡萄糖，但仍然发酵碳水化合物，这表明代谢倾向于糖原而不是葡萄糖。葡萄糖释放的证据进一步表明糖原在培养中分解。结论svdm比BHI更准确地模拟阴道乳杆菌的代谢活性和环境效应，特别是在酸化和乳酸生成方面。尽管阴道丝虫病在VDM中生长受限，但其特有的代谢途径仍然很明显。这些发现强调了VDM在模拟阴道微生物群关键代谢特征方面的价值，特别是在乳酸菌占主导地位或加德纳菌普遍存在的情况下。

{"title":"Metabolism of Lactobacillus and Gardnerella vaginalis in vaginal defined media","authors":"Victoria Horrocks , Charlotte K. Hind , J. Mark Sutton , Rachel M. Tribe , A. James Mason","doi":"10.1016/j.anaerobe.2025.102991","DOIUrl":"10.1016/j.anaerobe.2025.102991","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluates how well a vaginal defined medium (VDM) replicates the i<em>n vivo</em> metabolic behaviour of key vaginal microbiota members - <em>Lactobacillus crispatus</em>, <em>L. jensenii</em>, and diverse <em>Gardnerella vaginalis</em> isolates - compared to brain heart infusion (BHI) medium.</div></div><div><h3>Methods</h3><div>We used <sup>1</sup>H NMR spectroscopy to characterise metabolic profiles during <em>in vitro</em> growth of <em>Lactobacillus</em> and <em>Gardnerella</em> species in VDM and BHI. Differences in metabolite production, growth, acidification, and carbohydrate utilisation were assessed.</div></div><div><h3>Results</h3><div>Both <em>L.</em> <em>crispatus</em> and <em>L. jensenii</em> grow well in VDM, produce substantially more lactate than in BHI, and acidify the culture more strongly - better reflecting the low pH environment of <em>Lactobacillus</em>-dominant vaginal microbiota. In contrast, <em>G. vaginalis</em> grows less robustly in VDM than in BHI, though key metabolic traits such as the Bifidobacterium shunt and mixed acid fermentation (evidenced by formate production) are preserved. Notably, neither genus consume available glucose, yet still ferment carbohydrates, suggesting a metabolic preference for glycogen over glucose. Evidence of glucose release further indicates glycogen breakdown in culture.</div></div><div><h3>Conclusions</h3><div>VDM more accurately models the metabolic activity and environmental effects of vaginal <em>Lactobacillus</em> species than BHI, particularly in terms of acidification and lactate production. Although <em>G. vaginalis</em> growth is limited in VDM, its characteristic metabolic pathways remain evident. These findings underscore the value of VDM in modelling key metabolic features of the vaginal microbiota, especially under conditions where <em>Lactobacillus</em> dominate or <em>Gardnerella</em> is prevalent.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102991"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Odoribacter splanchnicus bacteremia: a rare condition associated with intestinal disease 内脏臭杆菌菌血症：一种与肠道疾病相关的罕见疾病。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-09-04 DOI: 10.1016/j.anaerobe.2025.102994

Hiroshi Umemura , Hirokazu Kobayashi , Yumiko Tanimichi , Hiroyuki Nishiyama , Natsumi Ikumi , Masaki Nakajima , Sachio Tsuchida , Tomohiro Nakayama

Odoribacter splanchnicus is an anaerobe that normally inhabits the human intestine and rarely causes infections in humans. In recent years, however, three cases of O. splanchnicus bacteremia have been reported. We retrospectively searched our laboratory information system and detected five cases of O. splanchnicus bacteremia in 2014–2024. All cases of O. splanchnicus bacteremia reported to date have been associated with intestinal infection, but our five cases included one associated with decubitus ulcer infection. Antimicrobial susceptibility testing results were available in four of our five cases. O. splanchnicus showed susceptibility to beta-lactams and metronidazole. However, one isolate was resistant to clindamycin, and another showed intermediate susceptibility to levofloxacin. Among our five cases and the three previously reported cases, no patient was using immunosuppressive agents or had immunocompromising diseases except for one case with malignancy. While O. splanchnicus bacteremia is certainly rare, more cases may be found by searching the laboratory information system as in this investigation. Further accumulation of cases will help to elucidate the pathogenesis of infections caused by O. splanchnicus, which is endemic in the gastrointestinal tract, and will reveal more information on antimicrobial susceptibility.

内脏臭杆菌是一种常见于人体肠道的厌氧菌，很少引起人体感染。然而，近年来报道了三例内脏O.菌血症。回顾性检索实验室信息系统，2014-2024年共检出5例内脏弓形虫菌血症。迄今为止报道的所有内脏单胞菌菌血症病例都与肠道感染有关，但我们的5例病例中有一例与褥疮感染有关。我们的5个病例中有4个获得了抗菌药物敏感性试验结果。对-内酰胺类和甲硝唑敏感。然而，一株对克林霉素耐药，另一株对左氧氟沙星有中等敏感性。在我们的5例病例和先前报道的3例病例中，除1例恶性肿瘤外，没有患者使用免疫抑制剂或患有免疫功能低下疾病。虽然O. spplanchnicus菌血症当然是罕见的，但在本调查中，通过搜索实验室信息系统可能会发现更多病例。进一步的病例积累将有助于阐明肠道地方病O. spplanchnicus感染的发病机制，并将揭示更多的抗菌药物敏感性信息。

{"title":"Odoribacter splanchnicus bacteremia: a rare condition associated with intestinal disease","authors":"Hiroshi Umemura , Hirokazu Kobayashi , Yumiko Tanimichi , Hiroyuki Nishiyama , Natsumi Ikumi , Masaki Nakajima , Sachio Tsuchida , Tomohiro Nakayama","doi":"10.1016/j.anaerobe.2025.102994","DOIUrl":"10.1016/j.anaerobe.2025.102994","url":null,"abstract":"<div><div><em>Odoribacter splanchnicus</em> is an anaerobe that normally inhabits the human intestine and rarely causes infections in humans. In recent years, however, three cases of <em>O. splanchnicus</em> bacteremia have been reported. We retrospectively searched our laboratory information system and detected five cases of <em>O. splanchnicus</em> bacteremia in 2014–2024. All cases of <em>O. splanchnicus</em> bacteremia reported to date have been associated with intestinal infection, but our five cases included one associated with decubitus ulcer infection. Antimicrobial susceptibility testing results were available in four of our five cases. <em>O. splanchnicus</em> showed susceptibility to beta-lactams and metronidazole. However, one isolate was resistant to clindamycin, and another showed intermediate susceptibility to levofloxacin. Among our five cases and the three previously reported cases, no patient was using immunosuppressive agents or had immunocompromising diseases except for one case with malignancy. While <em>O. splanchnicus</em> bacteremia is certainly rare, more cases may be found by searching the laboratory information system as in this investigation. Further accumulation of cases will help to elucidate the pathogenesis of infections caused by <em>O. splanchnicus</em>, which is endemic in the gastrointestinal tract, and will reveal more information on antimicrobial susceptibility.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102994"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Clostridioides difficile isolated from older adults during prospective population-based active surveillance at nine hospitals in Tokyo, Japan, 2018-2020. 2018-2020年日本东京9家医院前瞻性人群主动监测中老年人分离的艰难梭菌分子特征

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-07-17 DOI: 10.1016/j.anaerobe.2025.102985

Zhenghui Li, Ashlesh K Murthy, C Hal Jones, Kwok Lee, Urvi Rajyaguru, Deepika Athinarayanan, Lubomira Andrew, Richard V Goering, Shuhei Ito, Elisa Gonzalez, Pingping Zhang, Paul A Liberator, Warren V Kalina, Michael W Pride, Frederick J Angulo, Jennifer C Moïsi, Kazuhiro Tateda

Background: To characterize Clostridioides difficile isolates identified during a prospective multi-hospital population-based study of C. difficile infection (CDI).

Methods: Between December 2018 and March 2020, inpatients ≥50 years of age with new-onset diarrhea in nine Tokyo hospitals were investigated for CDI. Stool specimens were screened by C. DIFF QUIK CHEK COMPLETE® and glutamate dehydrogenase (GDH) positive stools tested by Xpert® C. difficile/Epi PCR assay. PCR positive stools were tested by cytotoxicity neutralization assay to determine presence of functional toxin. GDH positive stools were also anaerobically cultured and whole genome sequences of C. difficile isolates were acquired.

Results: Toxigenic C. difficile were isolated from 64 patients with CDI; 22 were RT018/356, 9 were RT369, 4 each were RT106 and RT002, 15 were other RTs, and 10 had an unknown RT. Four isolates were positive for binary toxin: one isolate each of RT027, RT078/126, RT080, and one unknown. In terms of the pathogenicity locus profile, 55 were tcdA+/tcdB+ and 9 were tcdA-/tcdB+. In terms of ST, 23 were ST17, nine were ST81, 31 were other STs, and one had a novel ST.

Conclusions: Consistent with prior studies, C. difficile isolates from patients with CDI represented diverse ribotypes with approximately one third of isolates RT018/356. Of note, one isolate each of RT027 and RT078/126 were identified, indicating these hypervirulent strains are present in hospitals in Japan. Public health interventions are needed to reduce the CDI burden in Japan.

背景：在一项基于多医院人群的艰难梭菌感染（CDI）前瞻性研究中鉴定出的艰难梭菌分离物。方法：对2018年12月至2020年3月东京9家医院住院≥50岁新发腹泻患者进行CDI调查。采用C. DIFF QUIK CHEK COMPLETE®筛选粪便标本，采用Xpert®艰难梭菌/Epi PCR检测谷氨酸脱氢酶（GDH）阳性粪便标本。采用细胞毒性中和试验检测PCR阳性粪便是否存在功能性毒素。GDH阳性的粪便也进行厌氧培养，并获得艰难梭菌分离株的全基因组序列。结果：64例CDI患者分离到产毒艰难梭菌；RT018/356 22株，RT369 9株，RT106和RT002各4株，其他rt各15株，未知rt 10株。4株双毒阳性，RT027、RT078/126、RT080各1株，未知1株。在致病性位点谱上，tcdA+/tcdB+ 55个，tcdA-/tcdB+ 9个。在ST方面，23例为ST17, 9例为ST81, 31例为其他ST， 1例为新型ST。结论：与先前的研究一致，CDI患者分离的艰难梭菌具有多种核糖型，约三分之一的分离株为RT018/356。值得注意的是，已鉴定出RT027和RT078/126各一株分离株，表明这些高毒力菌株存在于日本的医院中。日本需要采取公共卫生干预措施，以减轻CDI负担。

{"title":"Molecular characterization of Clostridioides difficile isolated from older adults during prospective population-based active surveillance at nine hospitals in Tokyo, Japan, 2018-2020.","authors":"Zhenghui Li, Ashlesh K Murthy, C Hal Jones, Kwok Lee, Urvi Rajyaguru, Deepika Athinarayanan, Lubomira Andrew, Richard V Goering, Shuhei Ito, Elisa Gonzalez, Pingping Zhang, Paul A Liberator, Warren V Kalina, Michael W Pride, Frederick J Angulo, Jennifer C Moïsi, Kazuhiro Tateda","doi":"10.1016/j.anaerobe.2025.102985","DOIUrl":"10.1016/j.anaerobe.2025.102985","url":null,"abstract":"<p><strong>Background: </strong>To characterize Clostridioides difficile isolates identified during a prospective multi-hospital population-based study of C. difficile infection (CDI).</p><p><strong>Methods: </strong>Between December 2018 and March 2020, inpatients ≥50 years of age with new-onset diarrhea in nine Tokyo hospitals were investigated for CDI. Stool specimens were screened by C. DIFF QUIK CHEK COMPLETE® and glutamate dehydrogenase (GDH) positive stools tested by Xpert® C. difficile/Epi PCR assay. PCR positive stools were tested by cytotoxicity neutralization assay to determine presence of functional toxin. GDH positive stools were also anaerobically cultured and whole genome sequences of C. difficile isolates were acquired.</p><p><strong>Results: </strong>Toxigenic C. difficile were isolated from 64 patients with CDI; 22 were RT018/356, 9 were RT369, 4 each were RT106 and RT002, 15 were other RTs, and 10 had an unknown RT. Four isolates were positive for binary toxin: one isolate each of RT027, RT078/126, RT080, and one unknown. In terms of the pathogenicity locus profile, 55 were tcdA+/tcdB+ and 9 were tcdA-/tcdB+. In terms of ST, 23 were ST17, nine were ST81, 31 were other STs, and one had a novel ST.</p><p><strong>Conclusions: </strong>Consistent with prior studies, C. difficile isolates from patients with CDI represented diverse ribotypes with approximately one third of isolates RT018/356. Of note, one isolate each of RT027 and RT078/126 were identified, indicating these hypervirulent strains are present in hospitals in Japan. Public health interventions are needed to reduce the CDI burden in Japan.</p>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":" ","pages":"102985"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Desulfovibrio desulfuricans bacteraemia and associated liver abscess: Case report and literature review 脱硫弧菌菌血症与肝脓肿：1例报告及文献复习。

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Anaerobe

Pub Date : 2025-10-01 Epub Date: 2025-05-30 DOI: 10.1016/j.anaerobe.2025.102973

Katharine Xiwen Huang , Justin Deane Jackson

We present a rare case of Desulfovibrio desulfuricans bacteraemia and associated liver abscess. A 72-year-old Caucasian woman presented with an acute history of fevers, rigors and abdominal pain. She was managed empirically with ceftriaxone and metronidazole and required 24 hours of inotropic support due to hypotension from sepsis. A computed tomography scan of her abdomen demonstrated a 6cm liver abscess in maximal diameter. On day seven of her admission, blood cultures flagged an anaerobic gram-negative bacillus, which was later identified by MALDI-TOF MS as Desulfovibrio desulfuricans. The isolate was susceptible to amoxicillin-clavulanic acid, ertapenem, moxifloxacin, clindamycin and metronidazole but resistant to piperacillin-tazobactam. Despite percutaneous drainage on day two of admission, her fevers persisted for three weeks. On day nine of admission, intravenous ceftriaxone and metronidazole were switched to amoxicillin-clavulanic acid, which was administered for a total of six weeks, resulting in clinical resolution. Our case highlights Desulfovibrio desulfuricans as an uncommon cause of liver abscess and one which is important to consider if culturing an anaerobic, spiral gram-negative bacilli. Empirical antibiotics need to be carefully considered as there may be resistance to beta-lactam antibiotics including piperacillin-tazobactam.

我们报告一例罕见的脱硫弧菌菌血症及肝脓肿。一名72岁白人女性，表现为急性发热、僵硬和腹痛。她经验性地使用头孢曲松和甲硝唑，由于败血症引起的低血压需要24小时的肌力支持。腹部计算机断层扫描显示最大直径为6cm的肝脓肿。入院第7天，血液培养提示一种厌氧革兰氏阴性杆菌，随后通过MALDI-TOF质谱鉴定为脱硫弧菌。该菌株对阿莫西林-克拉维酸、厄他培南、莫西沙星、克林霉素和甲硝唑敏感，对哌拉西林-他唑巴坦耐药。尽管入院第2天经皮引流，她的发烧持续了3周。入院第9天将头孢曲松、甲硝唑静脉注射改为阿莫西林-克拉维酸，共给药6周。脱硫弧菌是一种罕见的肝脓肿的病因，在厌氧的情况下，重要的是要考虑螺旋革兰氏阴性杆菌，它可以对包括哌拉西林-他唑巴坦在内的经验抗生素产生耐药性。

{"title":"Desulfovibrio desulfuricans bacteraemia and associated liver abscess: Case report and literature review","authors":"Katharine Xiwen Huang , Justin Deane Jackson","doi":"10.1016/j.anaerobe.2025.102973","DOIUrl":"10.1016/j.anaerobe.2025.102973","url":null,"abstract":"<div><div>We present a rare case of <em>Desulfovibrio desulfuricans</em> bacteraemia and associated liver abscess. A 72-year-old Caucasian woman presented with an acute history of fevers, rigors and abdominal pain. She was managed empirically with ceftriaxone and metronidazole and required 24 hours of inotropic support due to hypotension from sepsis. A computed tomography scan of her abdomen demonstrated a 6cm liver abscess in maximal diameter. On day seven of her admission, blood cultures flagged an anaerobic gram-negative bacillus, which was later identified by MALDI-TOF MS as <em>Desulfovibrio desulfuricans</em>. The isolate was susceptible to amoxicillin-clavulanic acid, ertapenem, moxifloxacin, clindamycin and metronidazole but resistant to piperacillin-tazobactam. Despite percutaneous drainage on day two of admission, her fevers persisted for three weeks. On day nine of admission, intravenous ceftriaxone and metronidazole were switched to amoxicillin-clavulanic acid, which was administered for a total of six weeks, resulting in clinical resolution. Our case highlights <em>Desulfovibrio desulfuricans</em> as an uncommon cause of liver abscess and one which is important to consider if culturing an anaerobic, spiral gram-negative bacilli. Empirical antibiotics need to be carefully considered as there may be resistance to beta-lactam antibiotics including piperacillin-tazobactam.</div></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"95 ","pages":"Article 102973"},"PeriodicalIF":2.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0